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Silica Colloidal Crystals As Emerging Materials For The High-Throughput Sizing Of Proteins By Packed Capillary Electrophoresis, Nadine K. Njoya

Open Access Dissertations

Protein heterogeneity is currently one of the leading problems in formulation of therapeutic proteins. The effectiveness of therapeutic proteins is diminished when they exhibit heterogeneity due to aggregates and degradation by-products. Furthermore, guidelines set forth by the FDA require therapeutic proteins to have high stability as well as high purity. With development time on the order of years and costs incurred in the millions, pharmaceutical companies are investing more time in finding fast and efficient ways to screen for protein heterogeneity. Current methods such as native polyacrylamide gel electrophoresis (PAGE), size-exclusion chromatography (SEC) and capillary electrophoresis (CE) are time consuming, …

Utilizing Electron Microscopy And Spectroscopy Methods To Understand Water Structure And Water Doping, Lior Miller

Open Access Dissertations

Water is the second most common element in the universe and the most studied material on earth. Most of the studies concerning water are from the fields of chemistry and biology. Hence, the structure of water molecules and short range order and interactions are well characterized and understood. However, the collective arrangement of water molecules and the long range order are still missing. Understanding of this long range order in water is needed, as it is the key to many water activities.

To fill this gap, this study utilizes a new direct method for characterization of water in the vapor …

Top-Down Interrogation And Characterization Of Biological Macromolecules, Yang Gao

Open Access Dissertations

Analytical mass spectrometry has been widely applied in the field of biomolecule identification and characterization. It provides fast, accurate and robust information relevant to the molecular mass, composition and structure of the target analytes.

This thesis is focused on the top-down analysis of various macrobiomolecules, nucleic acids and proteins, in the context of ion/ion reaction and gas-phase unimolecular dissociation. The first part of the thesis deals with gas phase fragmentation behavior of chemically modified oligonucleotides. Factors, such as the observation time-scale, the ion type and the energy deposition method, are explored using a quadrupole/time-of-flight mass spectrometer. Novel fragmentation pathway associated …

Structural Analysis Of Carbohydrates By Mass Spectrometry, Chiharu Konda

Open Access Dissertations

Protein glycosylation is a highly frequent post-translational modification. Glycosylation is actively involved in intermolecular and intercellular binding events that are important to a wide range of biological functions such as immunity and fertility. The unique functions of glycans are directly related to their structures. In order to fully characterize the structure of an unknown glycan, 5 levels of structural information is needed: sugar unit identity, anomeric configuration, linkage types, sequence, and branching location. One of the grand challenges limiting the advance of glycosciences is the lack of sensitive tools for detailed glycan structural analysis. Tandem mass spectrometry (MS/MS) is a …

Continued Progress Towards Efficient Syntheses Of Cephalostatin North 1 Analogs, Daniel Preston Jamieson

Open Access Dissertations

Jamieson, Daniel P. Ph.D., Purdue University, December 2013. Continued Progress Towards Efficient Syntheses of Cephalostatin North 1 Analogs. Major Professor: PhilipL. Fuchs.

The cephalostatins and ritterazines represent an intriguing class of marine natural products in both structure and biological activity, and the progress towards the synthesis of high valued analogs with a focus on process orientated methodology is described herein. Included in the pages to follow, is the further advancement of the `Red-Ox' strategy toward the highly efficient synthesis of 25-epi-4,15-dihydro-North 1, Chapter 2. A short review focusing on the earlier efforts toward the main synthetic challenges of North 1 …

Fluorous- Phase Synthesis Of Heparan Sulfate Disaccharides & Low-Molecular Weight Additives For Enhancing The Performance Of Lithium-Ion Batteries., Matthew Daniel Casselman

Open Access Dissertations

Heparan sulfate is a complex cell-surface proteoglycan that serves many important roles in biology, such as growth and development, immune response and pathogenesis. Heparan sulfate is structurally heterogeneous due to the variable post- glycosylation modifications, particularly the generation of diverse sets of sulfate esters (sulfoforms) for any given disaccharide unit. In order to establish useful relationships between heparan sulfate structure and biological activity, a set of well-defined sulfoforms is necessary to support binding affinity screening. In this thesis, we describe the generation of diverse sulfoforms from heparan disaccharides using a fluorous tag to facilitate purification of highly charged intermediates, and …

Osmium Catalyzed Dihydroxylation And Oxidative Cleavage Of Vinyl Sulfone And Elucidation Of The Vinylsulfone Polypropionate Methodology For The Synthesis Of The C32 Des-Methyl C28-C34 Actin Binding Tail Of Aplyronine A, Thomas Paul Bobinski

Open Access Dissertations

A general methodology for the generation of dipropionate functionalities using cyclic 7-membered vinylsulfones has been devised for the purpose of synthesizing polyketide natural products such as aplyronine A. Final oxidative cleavage via ozonolysis has been shown to be difficult providing variable yields. Furthermore, elegant synthesis of the C28-C34 segment of the aplyronine A actin binding tail has proved elusive. Utilization of OsO4 and catalytic citric acid has led to a methodology whereby harsh ozonolysis procedures can be mostly avoided. A reengineering of the vinylsulfone polypropionate methodology in conjunction with osmylation has been found to provide the actin binding tail under …

Novel Modulation Of Adenylyl Cyclase Type 2, Jason Michael Conley

Open Access Dissertations

Adenylyl cyclase isoforms are distinctly modulated by G protein subunits and are therefore hypothesized to be uniquely regulated by proteins that influence G protein signaling. Activator of G protein signaling 3 (AGS3) is a G protein modulator that binds Gαi subunits in the GDP-bound state, implicating AGS3 as an important regulatorof Gi-coupled receptor signaling. We studied the ability of AGS3 to modulate recombinant adenylyl cyclase(AC) type 1 and 2 signaling in HEK293 cells following both acute and persistent activation of the D _2Ldopamine receptor (D_2L DR). AGS3 expression modestly enhanced the potency of acute quinpirole-induced D _2LDR …

Molecular Characteristics Of The Water Soluable Organic Matter In Size Resolved Aerosols Collected Over The North Atlantic Ocean, Sarah Catherine Gurganus

Chemistry & Biochemistry Theses & Dissertations

Aerosol particulate matter is acknowledged to have effects on health and the environment and further investigation into the molecular characteristics of aerosols is necessary in order to fully understand the potential links between the molecular characteristics and the impacts that these particles have on health and the environment. Aerosols are often discussed with reference to their size because particles of different sizes can often be associated with different primary sources. This study presents a molecular-level chemical characterization of the water soluble organic matter fraction of 17 aerosol impactor samples representing 3 air mass influences (North American, North African, and marine) …

Nmr And Computational Characterization Of Protein Structure And Ligand Binding, Terrence Neumann

Dissertations (1934 -)

Nuclear magnetic resonance (NMR) techniques combined with computational methods such as docking and cheminformatics were used to characterize protein structure and ligand binding. The thioredoxin system of Mycobacterium tuberculosis consists of a thioredoxin reductase and at least three thioredoxins. This system is responsible for maintaining the cellular protein thiol redox state in normal state. This maintenance is important as the bacterium is engulfed by the human macrophage. Here it is bombarded by reactive oxygen and nitrogen species in an attempt to disrupt normal cellular function in part by perturbing the protein thiols. To this end, the solution structures of the …

Synthesis, Characterization And Application Of Two-Dimensional Layered Metal Hydroxides For Environmental Remediation Purposes, Cleopas Machingauta

Dissertations (1934 -)

Two-dimensional layered nano composites, which include layered double hydroxides (LDHs), hydroxy double salts (HDSs) and layered hydroxide salts (LHSs) are able to intercalate different molecular species within their gallery space. These materials have a tunable structural composition which has made them applicable as fire retardants, adsorbents, catalysts, catalyst support materials, and ion exchangers. Thermal treatment of these materials results in destruction of the layers and formation of mixed metal oxides (MMOs) and spinels. MMOs have the ability to adsorb anions from solution and may also regenerate layered structures through a phenomenon known as “memory effect”. Zinc-nickel hydroxy nitrate was used …

Syntheses And Structures Of Iron(Ii) And Cobalt(Ii) Complexes Of Substituted Tetra(Pyrazolyl)Lutidine Derivatives, Heidi Marie Tatlock

Master's Theses (2009 -)

Pentadentate ligands provide stability to metal complexes as well as a binding site for substrates that make them exceptionally useful for studying reactions. They have been used in biomimetic studies, water splitting, and small molecule activation studies. A series of six pentadentate ligands derived from a,a,a',a'-tetra(pyrazolyl)lutidine, pz4lut, with methyl substituents decorating the ligand periphery has been synthesized. These ligands were coordinated to FeIICl, and the electronic and structural properties of the resulting complexes were studied in order to deduce the effects of methyl substitution at the 3-, 4-, and 5-positions of the pyrazolyl groups and at the methine positions. When …

Structural Evidence: A Single Charged Residue Affects Substrate Binding In Cytochrome P450 Bm-3, Jaclyn Catalano, Kianoush Sadre-Bazzaz, Gabriele A. Amodeo, Liang Tong, Ann Mcdermott

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Cytochrome P450 BM-3 is a bacterial enzyme with sequence similarity to mammalian P450s that catalyzes the hydroxylation of fatty acids with high efficiency. Enzyme-substrate binding and dynamics has been an important topic of study for cytochromes P450 because most of the crystal structures of substrate-bound structures show the complex in an inactive state. We have determined a new crystal structure for cytochrome P450 BM-3 in complex with N-palmitoylglycine (NPG), which unexpectedly showed a direct bidentate ion pair between NPG and arginine 47 (R47). We further explored the role of R47, the only charged residue in the binding pocket in cytochrome …

Pengaruh Pendekatan Genius Learning Berbasis Kerja Ilmiah Terhadap Prestasi Belajar Fisika Ditinjau Dari Pengetahuan Awal Siswa Smpn 13 Balikpapan Tahun 2012, Suliyono Suliyono

Jurnal Pendidikan Sains

Penguasaan konsep-konsep fisika siswa akan lebih optimal apabila pendidik menerapkan strategi pembelajaran yang mampu meningkatkan keaktifan dan motivasi belajar siswa dengan tetap berlandaskan pada pendekatan konstruktivis. Pendekatan Genius Learning Berbasis Kerja Ilmiah (GLBKI) diyakini mampu menjawab tuntutan perkembangan pendidikan serta mempermudah siswa dalam mempelajari konsep-konsep fisika. Penelitian ini bertujuan untuk menguji perbedaan prestasi belajar fisika antara siswa yang belajar menggunakan pendekatan Genius Learning Berbasis Kerja Ilmiah dan siswa yang belajar menggunakan pembelajaran konvensional. Penelitian ini menggunakan desain eksperimen dengan pemberian perlakuan berupa pendekatan GLBKI pada kelas eksperimen yang dipilih secara acak, sedangkan kelas kontrol diberikan pembelajaran konvensional. Data prestasi belajar …

Penggunaan Media Bangun Geometri Untuk Menanamkan Konsep Penjumlahan Pecahan, Nanik Ulfa

Jurnal Pendidikan Sains

Pemahaman konsep merupakan aspek penting dalam pembelajaran matematika. Untuk menanamkan pemahaman konsep pada siswa yang masih berada pada tahap berpikir konkret, diperlukan bantuan media pembelajaran yang akrab dengan siswa. Dalam penelitian ini, media yang digunakan adalah media bangun geometri bidang. Penggunaan media tersebut diharapkan mampu menciptakan suasana pembelajaran yang menyenangkan serta mendorong keterlibatan aktif siswa dalam proses pembelajaran. Pembelajaran dilaksanakan melalui tiga tahap, yaitu: (1) tahap awal untuk mengecek pengetahuan prasyarat siswa, (2) tahap inti, pada tahap ini siswa memanipulasi media bangun geometri sesuai dengan panduan dalam Lembar Kerja Siswa (LKS) yang disusun oleh peneliti, dan (3) tahap penutup, yaitu …

Peer Mediated Instruction And Intervention (Pmii) Tipe Classwide Peer Tutoring (Cwpt) Dan Kemampuan Akademik Pada Pembelajaran Ipa Biologi Smk, Hamsiah Hamsiah

Jurnal Pendidikan Sains

Pembelajaran IPA di SMKN 1 Bontang masih didominasi dengan strategi belajar konvensional yaitu metode belajar dengan ceramah. Hal ini berdampak terhadap hasil belajar kognitif IPA biologi yang cenderung masih rendah karena siswa belum terlatih menjadi pebelajar yang mandiri, sehingga inovasi pembelajaran PMII tipe CWPT dapat digunakan sebagai terobosan untuk mengembangkan hasil belajar kognitif. Penelitian ini dilakukan untuk mengetahui penerapan strategi CWPT dan kemampuan akademik pada pembelajaran IPA Biologi SMK. Penelitian eksperimen semu dengan rancangan pretest-posttest Nonequivalent Control Group. Hasil penelitian, yaitu: (1) ada pengaruh CWPT terhadap hasil belajar kognitif, (2) ada pengaruh kemampuan akademik terhadap hasil belajar kognitif, dan (3) …

Pengaruh Model Pembelajaran Arias Dan Arias Dipadu Peta Konsep Terhadap Kemampuan Berpikir Kritis, Kognitif, Dan Afektif, Ismail Ismail

Jurnal Pendidikan Sains

Model pembelajaran Assurance, Relevance, Interest, Assessment, dan Satisfaction (ARIAS) dipadu peta konsep dapat meningkatkan kemampuan berpikir kritis, kognitif, dan afektif. Penelitian bertujuan untuk menguji pengaruh model pembelajaran ARIAS, ARIAS dipadu peta konsep, dan peta konsep terhadap kemampuan berpikir kritis, kognitif, dan afektif. Data penelitian dikumpulkan melalui tes uraian dan lembar observasi. Hasil analisis data menunjukkan bahwa nilai rata-rata kemampuan berpikir kritis, kognitif dan afektif untuk kelompok model pembelajaran ARIAS dipadu Peta Konsep tergolong kategori sangat baik, kelas perlakuan pembelajaran ARIAS, dan kelas perlakuan Peta Konsep termasuk kategori baik.

Keefektifan Gambar Statis, Gambar Dinamis Ball-And-Stick, Dan Model Molekul Sederhana Yang Dibuat Dari Jarum Pentul Pada Pembelajaran Bentuk Dan Kepolaran Molekul, Muhammad Ardiansyah

Jurnal Pendidikan Sains

Tujuan penelitian ini adalah untuk membandingkan keefektifan gambar statis, gambar dinamis ball-and-stick, dan model molekul sederhana dibuat dari jarum pentul ditinjau dari hasil belajar kognitif siswa pada pembelajaran bentuk dan kepolaran molekul. Data penelitian adalah skor hasil belajar kognitif siswa yang dikumpulkan dengan menggunakan tes pilihan ganda yang terdiri dari 35 item dengan validitas isi tes sebesar 87,6%, dan koefisien reliabilitas, dihitung dengan rumus KR-21, sebesar 0,90. Data penelitian dianalisis menggunakan statistik uji t. Hasil penelitian menunjukkan bahwa skor hasil belajar kognitif siswa yang diperoleh dari pembelajaran bentuk dan kepolaran molekul dengan menggunakan model molekul sederhana dibuat dari jarum pentul …

Efektivitas Sekolah Menengah Kejuruan Berstandar Iso 9001:2008 Terhadap Pencapaian Standar Isi, Standar Proses, Dan Standar Kompetensi Lulusan, Yeni Ratih Pratiwi

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk mengetahui perbedaan efektivitas pencapaian standar isi, standar proses, dan standar kompetensi lulusan pada Sekolah Menengah Kejuruan (SMK) yang telah menerapkan standar mutu ISO 9001:2008 dan SMK yang belum menerapkannya, baik pada sekolah negeri maupun swasta. Pengumpulan data dilakukan menggunakan kuesioner tertutup dengan skala Likert. Analisis data menggunakan uji ANOVA satu arah dengan bantuan program SPSS. Hasil penelitian menunjukkan bahwa terdapat perbedaan efektivitas antara SMK negeri berstandar ISO dan SMK swasta berstandar ISO (p = 0,001), antara SMK negeri berstandar ISO dan SMK negeri belum berstandar ISO (p = 0,000), serta antara SMK negeri berstandar ISO dan …

Relevansi Soft Skill Yang Dibutuhkan Dunia Usaha/Industri Dengan Yang Dibelajarkan Di Sekolah Menengah Kejuruan, Didik Suryanto

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk mendeskripsikan relevansi soft skill yang dibutuhkan oleh dunia usaha dan industri dengan soft skill yang dibelajarkan di Sekolah Menengah Kejuruan (SMK) di Kabupaten Blitar. Instrumen penelitian yang digunakan berupa angket atau kuesioner. Data dianalisis menggunakan teknik analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa terdapat sejumlah soft skill yang relevan antara kebutuhan dunia usaha/industri dan pembelajaran di SMK menurut persepsi guru dan siswa, yaitu: (a) kejujuran dan sikap perilaku yang baik, (b) rasa tanggung jawab, (c) disiplin waktu, (d) kemampuan bekerja secara aman, (e) ketangguhan atau kegigihan dalam bekerja, (f) kemampuan mengatasi stres, (g) kemandirian atau tidak …

Pengaruh Penggunaan Peta Konsep Dalam Pembelajaran Inkuiri Terbimbing Ditinjau Dari Kemampuan Bernalar Siswa Kelas Xi, Agung Wahyudi

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk: (1) mengetahui perbedaan hasil belajar fisika antara siswa yang belajar menggunakan pembelajaran inkuiri terbimbing melalui peta konsep (PITPK) dan siswa yang belajar menggunakan pembelajaran inkuiri terbimbing (PIT); (2) menguji interaksi antara model pembelajaran dan kemampuan bernalar terhadap hasil belajar fisika; (3) mengetahui efektivitas PITPK dibandingkan PIT pada siswa dengan kemampuan bernalar tinggi; dan (4) mengetahui efektivitas PITPK dibandingkan PIT pada siswa dengan kemampuan bernalar rendah. Penelitian ini menggunakan rancangan faktorial 2 × 2 dengan teknik pengambilan sampel simple random sampling. Instrumen penelitian meliputi instrumen perlakuan berupa silabus, RPP, dan lembar observasi keterlaksanaan pembelajaran, serta instrumen pengukuran …

Pengembangan Media Pembelajaran Interaktif Untuk Membangun Pemahaman Konsep Garis Singgung Persekutuan Dua Lingkaran, Vivin Nur Afidah

Jurnal Pendidikan Sains

Media pembelajaran interaktif yang dikembangkan adalah media pembelajaran interaktif yang dirancang berdasarkan karakteristik pembelajaran dengan bantuan komputer/Computer Assisted Instruction (CAI) tipe tutorial. Dirancang berdasarkan teori beban kognitif yaitu meminimalkan pemrosesan beban kognitif extraneous, mengatur pemrosesan beban kognitif intrinsic, dan mengembangkan pemrosesan beban kognitif germane. Penelitian pengembangan mengikuti model pengembangan 4D yaitu define (pendefinisian), design (perancangan), develop (pengembangan), dan disseminate (penyebarluasan). Simpulannya adalah (1) hasil validasi oleh ahli dan praktisi menilai media pembelajaran interaktif yang dikembangkan valid, (2) hasil pengamatan aktivitas guru dan siswa menunjukkan media pembelajaran interaktif memenuhi kriteria kepraktisan, (3) hasil kuis menunjukkan sebagian besar siswa mencapai tingkat pemahaman …

Pengembangan Buku Kerja Materi Eksponen Bercirikan Rme Untuk Siswa Smk Teknik, Sri Widowati

Jurnal Pendidikan Sains

Tujuan penelitian pengembangan ini adalah menghasilkan buku kerja berbahasa Inggris materi eksponen yang bercirikan Realistic Mathematics Education (RME) untuk siswa Sekolah Menengah Kejuruan (SMK) teknik kelas X serta memenuhi kriteria valid, praktis dan efektif. Proses validasi ahli dilakukan untuk mengetahui tingkat kevalidan buku kerja yang dihasilkan. Proses uji coba dilakukan untuk mengetahui tingkat kepraktisan dan keefektifan dari buku kerja yang dihasilkan. Berdasarkan proses validasi, diperoleh hasil bahwa buku kerja yang dihasilkan memenuhi kategori valid. Sedangkan dari proses uji coba diperoleh bahwa keterlaksanaan pembelajaran dengan menggunakan buku kerja berada pada kriteria praktis. Selain itu, rata-rata siswa memberikan respon positif dan hasil …

Degradasi Anaerob Sampah Organik Dengan Bioaktivator Effective Microorganism-5 (Em-5) Untuk Menghasilkan Biogas, Metri Dian Insani

Jurnal Pendidikan Sains

Tujuan penelitian ini adalah untuk: (1) menganalisis perbedaan penggunaan tongkol jagung, kelaras pisang, dan kulit pisang dengan penambahan kotoran sapi terhadap tekanan biogas, (2) menganalisis perbedaan penggunaan tongkol jagung, kelaras pisang, dan kulit pisang dengan penambahan kotoran sapi terhadap lama waktu nyala api biogas yang dihasilkan, dan (3) menganalisis perbedaan penggunaan tongkol jagung, kelaras pisang, dan kulit pisang dengan penambahan kotoran sapi terhadap rasio C/N akhir. Penelitian eksperimen didesain menggunakan rancangan acak lengkap (RAL), dengan tiga perlakuan masing-masing dalam 3 kali ulangan. Hasil penelitian membuktikan bahwa: (1) terdapat perbedaan tongkol jagung, kelaras pisang, dan kulit pisang dengan penambahan kotoran sapi …

Pengaruh Penerapan Model Pembelajaran Pemerolehan Konsep Dipadu Pembelajaran Kooperatif Stad Terhadap Prestasi Belajar, Keaktifan, Dan Respons Siswa Pada Pembelajaran Ikatan Kimia, Putri Ridha Ilahi

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk mengetahui perbedaan: (1) prestasi belajar siswa menggunakan model pembelajaran pemerolehan konsep dipadu pembelajaran kooperatif STAD dan prestasi belajar siswa menggunakan model pembelajaran pemerolehan konsep dalam pembelajaran konvensional; (2) keaktifan siswa menggunakan model pembelajaran pemerolehan konsep dipadu pembelajaran kooperatif STAD dan keaktifan siswa menggunakan model pembelajaran pemerolehan konsep dalam pembelajaran konvensional; (3) respon siswa terhadap pembelajaran pemerolehan konsep dipadu pembelajaran kooperatif STAD dan respon siswa terhadap pembelajaran pemerolehan konsep dalam pembelajaran konvensional. Penelitian ini menggunakan rancangan penelitian deskriptif dan rancangan eksperimen semu (quasy experiment design). Data prestasi belajar dikumpulkan menggunakan tes objektif dan koefisien reliabilitas dihitung dengan …

Catalytic Reactions For The Production Of Biomarkers From The Biological Warfare Agent Anthrax (Dr.), Jason E. Hawkes, Dr. Milton L. Lee

Journal of Undergraduate Research

Bacillus anthracis, more commonly known as anthrax, poses a serious threat to the national security of the United States of America. In its weaponized form, anthrax spores are easily aerosolized, demonstrate long residence times in the atmosphere (typically variable for several days), and can be fatal if ingested or inhaled into the lungs. Following ingestion or inhalation, rapid growth and reproductive activity is triggered, releasing large amounts of deadly toxin. The lethal dose of anthrax spores is very small, approximately 10 nanograms of spores.1 As a result of its easy dispersal, long residence times, and high toxicity, anthrax is becoming …

Synthesizing Racemic And Optical Active 6-Methylsulfinylhexyl Isothiocyanate, Bo Duan, Dr. Merritt Andrus

Journal of Undergraduate Research

6–methylsulfinylhexyl isothiocyanate (MS-ITC) is the active component of wasabi, Japanese horseradish. Wasabi plant (Wasabia Japonica), a member of the Cruciferae family, is native to Japan and is traditionally found growing in or by cold mountain streams. The root and stem of this plant has a fiery hot flavor that quickly dissipates in the mouth, leaving a lingering sweet taste.

Chemical Isolation From Plant Endophytes, David Dahl, Dr. Steven Wood

Journal of Undergraduate Research

The major research that I have worked with is on the extraction of biologically active compounds from plant endophytes native to the west desert of Utah and high altitude Paramo of Ecuador. For a year I have been working the Department of Chemistry and Biochemistry’s Natural Product Research Group under Dr. Noel Owen and Dr. Steven Wood. I planned and am executing a project that combines several different disciplines; anthropology, botany, and chemistry.

Engineering Small Molecule-Dependent Tools For The Study Of Protein Function In Vivo, Jonathan Dewey, Dr. Allen R. Buskirk

Journal of Undergraduate Research

Analyzing the function of newly discovered genes and the proteins that they encode is crucial to the advancement of genetics, cell and molecular biology, and medicine. Scientists have implemented various methods to study individual proteins within a cell, including genetic and small-molecule dependent approaches. Our goal is to combine the generality of genetic manipulation with the time-sensitivity of small-molecule dependence to create tools to study the function of Hedgehog pathway proteins and validate them as drug targets.

Three-Dimensional Modeling Of Poliovirus Structure And Activity, Roane Noel, Dr. David Belnap

Journal of Undergraduate Research

The Poliovirus image to the left is from a collection of three-dimensionally reconstructed images of virus particles found at the website: http://www.cgl.ucsf.edu/Research/virus/capsids/viruses.html October 10, 2005. This image is identical to the type of Poliovirus image I reconstructed with the help of mentor Dr. David Belnap.