Digital Commons Network^™

Yeast Fkbp-13 Is A Membrane-Associated Fk506-Binding Protein Encoded By The Nonessential Gene Fkb2, J. B. Nielsen, Forrest Foor, John Siekierka, M. J. Hsu, Naasa Ramadan, Nancy Morin, Ali Shafiee, Arlene M. Dahl, Leonardo Brizuela, Gary L. Chrebet, Keith A. Bostian, Stephen A. Parent

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

The immunosuppressants FK506 and rapamycin prevent T-cell activation and also inhibit the growth of certain strains of the yeast Saccharomyces cerevisiae. It has previously been shown that yeast contains a 12-kDa cytosolic FK506-binding protein (yFKBP-12), which also possesses peptidylprolyl cis-trans isomerase activity, and that fkb1 strains lacking yFKBP-12 are resistant to rapamycin and sensitive to FK506. The absence of yFKBP-12 permitted the detection and isolation of a second FK506- and rapamycin-binding protein, which is about 13 kDa in size (yFKBP-13) and membrane-associated. Purified yFKBP-13 binds FK506 with 15-fold lower affinity than yFKBP-12 and has peptidylprolyl cis-trans isomerase activity with a …

Remote Dianions In The Synthesis Of Indolizidine Alkaloids, Diana L. C. Green Green

Dissertations

No abstract provided.

Syntheses Of Azure-B Neuropeptide Conjugates And A Novel Bicyclic Triene, Milind D. Choubal

Dissertations

No abstract provided.

Structure Of (22S)-3Β-Acetoxy-20-(3-Isopropylisoxazolin-5-Yl)-4,4,14 Α-Trimethylpregn-8(9)-Ene, Sergey V. Lindeman, Mikael S. Alexanyan, Yuri T. Struchkov, Rajesh Kumar Thaper, Irina G. Reshetova, Alexey V. Kamernitzky

Chemistry Faculty Research and Publications

C₃₂H₅₁NO₃, M_r = 497·7, orthorhombic, P2₁2₁2₁, a = 7·577 (2), b = 10·510 (2), c = 35·399 (7) Å, V = 2819 (1) ų, Z = 4, D_x = 1·173 g cm^-3, λ (Mo Kα) = 0·71073 Å, μ = 0·69 cm^-1, F(000) = 1096, T = 153 K, R = 0·0497 for 2235 observed reflections. The compound investigated is found to be a (22S)-epimer.

Resonance Raman Spectra Of Chloroperoxidase Reaction Intermediates, Ann Marie G. Sullivan

Theses and Dissertations

Chloroperoxidase is an enzyme that exhibits spectroscopic and structural properties similar to cytochrome P-450. Chloroperoxidase is studied using resonance Raman spectroscopy to characterize the reaction intermediates of the physiological mechanism, known as compounds I and II. Compound I is formed by a two electron oxidation of the resting enzyme and contains an Fe(IV) porphyrin ℼ cation radical. A one electron reduction of compound I produces the compound II intermediate which contains an oxy-ferryl [Fe(IV)=O] iron heme.

Chloroperoxidase is a heme enzyme of substantial interest because of its structural similarity to cytochrome P-450 and because of its diverse reactivity. Chloroperoxidase can …

Melbourne Automobile Emissions Study, Donald H. Stedman, Yi Zhang

Fuel Efficiency Automobile Test Publications

No abstract provided.

On-Road Remote Sensing Of Carbon Monoxide And Hydrocarbon Emissions During Several Vehicle Operating Conditions (Presentation), Lowell L. Ashbaugh, Douglas R. Lawson, Gary A. Bishop, Paul L. Guenther, Donald H. Stedman, Robert D. Stephens, Peter J. Groblicki, Jayendra S. Parikh, Brian J. Johnson, Steve C. Huang

Fuel Efficiency Automobile Test Publications

No abstract provided.

Magnetic Properties Of A Novel Iron Carbide Film, Fe₇C₃X Formed In A Glow Discharge, Oran Allan Pringle, Gary J. Long, Fernande Grandjean, Jun L. Li, George C. Hadjipanayis, William Joseph James

Physics Faculty Research & Creative Works

No abstract provided.

Structural Studies Of Rare Earth-Iron Nitrides By Neutron Powder Diffraction, William B. Yelon, George C. Hadjipanayis

Physics Faculty Research & Creative Works

No abstract provided.

Preparation Of Electron Donor And Acceptor Molecules For Porphyrin Derivatization, Christoph Hoefler

Dissertations and Theses

Porphyrins derivatized with electron donating and electron withdrawing groups can be used for artificial photosynthesis. Four new compounds, two electron donors and two electron acceptors, have been synthesized for prospective porphyrin linkages.

New Reaction Conditions For The Synthesis Of Wieland-Miescher Ketone, Jonathan J. Bates

Presidential Scholars Theses (1990 – 2006)

Natural products chemistry is a very important field of chemistry to chemists, biochemists, and biologists. The ability to synthesize a naturally occurring chemical in the laboratory is necessary for some experiments. Often it is difficult to isolate a large quantity of a compound from its natural source when needed for certain experiments. Established procedures for artificial synthesis of this compound can produce higher yields. Sometimes, the function of a natural product is tested by observing the behavior of analogues in the same biological system. The differences in behavior can be related to differences in structure . Since such analogues are …

Improved Recovery Of A Radlolabeled Peptide With An Albumin-Treated Reversed-Phase Hplc Column, David S. Hage, Robert L. Taylor, Pai C. Kao

David Hage Publications

Reversed-phase high-performance liquid chromatography (RP-HPLC) is an important tool in the purification of radiolabeled peptides and proteins for immunoassay. However, for some proteins and peptides it is difficult to achieve reproducible behavior in RP-HPLC because of the low recovery of these compounds. Factors that can be varied to improve recovery include the strength or pH of the mobile phase, the chain length and spacing of groups on the reversed-phase support, and the flow rate or steepness of the elution gradient (1-5). ... In summary, we obtained better recovery and more reproducible chromatographic behavior for labeled 1-34 PTHrP with an albumin-pretreated …

Intact Parathyroid Hormone: Performance And Clinical Utility Of An Automated Assay Based On High-Performance Immunoaffinity Chromatography And Chemiluminescence Detection, David S. Hage, Bob Taylor, Pai C. Kao

David Hage Publications

The performance and clinical utility of an automated assay of intact parathyroid hormone (parathyrin, PTH) are evaluated. The method is based on the extraction of PTH from plasma by an HPLC column containing immobilized anti-(44-68 PTH) antibodies. The PTH retained is detected with a postcolumn reactor and use of anti-(1--34 PTH) chemiluminescent-labeled antibodies. The total cycle time of the assay is 6.5 mm per injection after a 1-h incubation.The lower limit of detection for PTH in a 66-pL plasma sample was 0.5 pmol/L based on peak heights and 0.2 pmol/L based on peak areas. Mean analytical recovery for PTH added …

Empirical Procedure That Uses Molecular Structure To Predict Enantioselectivity Of Chiral Stationary Phases, Alain Berthod, San Chun Chang, Daniel W. Armstrong

Chemistry Faculty Research & Creative Works

A total of 121 racemic compounds were separated In the normal-phase mode on a (S)-(1-naphthylethyl)carbamoylated β-cyclodextrin (S-NEC-β-CD) bonded phase and 74 on the R equivalent (-NEC) chiral stationary phase (CSP). All compounds are of the type that have four substituents on a stereogenlc center, rather than an "axis of chirality". It is shown that the binary solvent pair used as the mobile phase has a significant Influence on chiral recognition. However, the proportions of the components of a specific pair have little effect. From the results, the Individual contributions to chiral recognition by these CSPs were estimated for 81 different …

Multiple Enantioselective Retention Mechanisms On Derivatized Cyclodextrin Gas Chromatographic Chiral Stationary Phases, Alain Berthed, Weiyong Li, Daniel W. Armstrong

Chemistry Faculty Research & Creative Works

2, 6-Di-O-pentyl-3-O-(trifluoroacetyl) (DP-TFA) derivatized β and γ-cyclodextrlne are able to resolve a wide variety of volatile racemic compounds. The enantiomeric recognition mechanism of these phases was Investigated. The retention behavior of homologous series showed that lengthening the side alkyl chain Increases the retention time but does not affect enantioselectivlty. The thermodynamic parameters, free energy, enthalpy, and the difference In free energy, enthalpy, and entropy between enantiomers were evaluated for 24 enantiomeric pairs. From this data, It appears that the compounds can be arranged In two groups. One group has high values for enthalpy, entropy, free energy, and the corresponding difference …

Fundamental Aspect And Behavior Of Saturated Fluorocarbons In Glow Discharge In Absence Of Potential Source Of Hydrogen, Yu Iriyama, H. Yasuda

Chemistry Faculty Research & Creative Works

The glow discharge of a series of saturated fluorocarbons, C_nF_2n+2 (n = 1, 2, 4, 6, and 8), was studied with glass substrates which do not contain any hydrogen. It was found that the deposition rate was a function of the F/C ratio of the starting fluorocarbons. That is, fluorocarbons with higher F/C ratio, such as CF₄ and C₂F₆, hardly polymerized, while fluorocarbons with lower F/C ratio, such as C₈F₁₈, polymerized as well as C₂F₄. After plasma exposure, the surface of glass substrate was …

Study Of The Hexamethylenetetramine, Ammonia, And Formaldehyde System: Quantitative Determinations, Gary Lee Madsen

Dissertations

No abstract provided.

Lithium Transport, Mg2+ Competition, And Phospholipid Composition In Human Erythrocytes: A Multinuclear Magnetic Resonance Study, Aida Abraha

Dissertations

No abstract provided.

Spin Label Studies Of Radical Reduction In Normal And Sickle Erythrocyte Membranes Associated With Antioxidants, Yin Zhang

Dissertations

No abstract provided.

The Synthesis And Chemistry Of N-Chlorosulfenylaziridines, Michael Claude Hare

Dissertations and Theses

Thiazyl chloride, the monomer of trichlorotrithiazene, reacts with fluorinated olefins to form N-chlorosulfenylaziridines. Four members of this new class of compounds have been prepared. Fluorine NMR, carbon-13 NMR, mass spectrometry, infrared spectroscopy, and elemental analysis have been used to characterize these compounds.

Hydration Behavior Of Laser Dye Aerosols Of Mixed Composition Having High Critical Supersaturations, Max B. Trueblood, Donald E. Hagen, Darryl J. Alofs

Mechanical and Aerospace Engineering Faculty Research & Creative Works

This Study Concerns Aerosol Particles of Mixed Composition. the Particles Contain a Water Insoluble Core (Polystyrene Latex), Surrounded by a Water Soluble Shell (Various Fluorescent Dyes). a Collison Nebulizer and an Electrostatic Aerosol Classifier Are Used to Prepare the Aerosol; its Critical Supersaturation Spectrum is Measured with a Diffusion Cloud Chamber. Experiments Were Done using Four Different Dyes (Disodium Fluorescein, Sulforhodamine 640, Sulforhodamine B and Rhodamine 560 Chloride) with Five Different Polystyrene Latex Sphere Sizes. the Results Indicate that a Kohler-Type Behavior is Obeyed. This Technique of Putting a Soluble Coating Onto an Insoluble Core is Found to Be a …

Experiments In Aerobic And Anaerobic Biodegradation Of Trichloroethylene, Qiong Liao

Theses

The removal of trichloroethylene (TCE) from industrial waste has been intensively studied in recent years, Biodegradation has been determined to be an effective treatment for TCE in the environment. Many researchers have studied the biodegradation of TOE and obtained successful results based on the use of specific isolated bacteria. At the NJIT Biotechnology Laboratory, a new method is being developed in which immobilized activated sludge is used to degrade several toxic chemicals in various reactor configurations. The objective of this research is to determine if these methods can be extended to treat TCE.

Activated sludge from a wastewater treatment plant …

Paint Stripper Composition Having Reduced Volatility Containing Decanolactone, N-Methylpyrrolidone And Butyrolactone And Method Of Use, Harvest L. Collier, Gary L. Bertrand, Raymond L. Venable

Chemistry Faculty Research & Creative Works

An improved composition of matter and method of use for removing cured coatings of paint or varnish or other similar coatings from substrates. The composition comrpises chlorinated solvent paint strippers (especially methylene chloride (CH₂CI₂)), waxes and an additive selected from the group consisting of decanolactone (C₁₀H₁₈O₂), N-methylpyrrolidone (CH₃NCH₂CH₂CH₂CH₂) and butyrolactone (OCH₂CH₂CH₂CO). The additive retards the evaporation of the solvent resulting in a composition that is environmentally less hazardous to a user and also resulting …

Resolution Of Enantiomeric Hydrocarbon Biomarkers Of Geochemical Importance, Daniel W. Armstrong, Yubing Tang, Janusz Zukowski

Chemistry Faculty Research & Creative Works

Chlral isoprenoid and hydroaromatic compounds occur in all crude oils, coals, shales, and most sediments. Many of these compounds are referred to as biological markers since they are thought to be derived from biological sources and their presence, relative concentrations, or stereochemistry can provide information as to a geological deposit's age, maturity, diagenetic history, and so forth. Because of the previous lack of effective and efficient analytical methodologies for resolving hydrocarbon enantiomers, the stereochemical information encoded in these molecules is largely untouched. A series of derivatized α-, β-, and γ-cyclodextrin chiral stationary phases (CSPs) were used for the gas chromatographic …

Surface Structure Of √3x√3r 30 Cl/Ni(111) Determined Using Low-Temperature Angle-Resolved-Photoemission Extended Fine Structure, Li-Qiong Wang, Z. Hussain, Z. Q. Huang, A. E. Schach Von Wittenau, Dennis W. Lindle, D. A. Shirley

Chemistry and Biochemistry Faculty Research

A surface structural study of the √3 × √3 R30° Cl/Ni(111) adsorbate system was made using low-temperature angle-resolved photoemission extended fine structure. The experiments were performed along two emission directions, [111] and [110], and at two temperatures, 120 and 300 K. The multiple-scattering spherical-wave analysis determined that the Cl atom adsorbs in the fcc threefold hollow site, 1.837(8) Å above the first nickel layer, with a Cl-Ni bond length of 2.332(6) Å, and an approximate 5% contraction between the first and the second nickel layers (the errors in parentheses are statistical standard deviations only).

Electron-Energy And –Angular Distributions In The Double Photoionization Of Helium, R. Wehlitz, Franz Heiser, Oliver Hemmers, B. Langer, A. Menzel, U. Becker

Environmental Studies Faculty Publications

Photoelectron spectra of helium have been measured at different angles and at various energies above the double-ionization threshold up to 120 eV to investigate the behavior of the energy and angular distributions, of shake-off electrons. Both energy and angular distributions clearly show a U-shaped profile which turns to a flat curve near threshold pointing to a uniform intensity distribution over the kinetic energy for all angles in this excitation energy regime. Our results for the angular-distribution asymmetry parameter indicate qualitative agreement with theoretical predictions but fail to provide them quantitatively.

Consumption Rates, Evacuation Rates And Diets Of Pygmy Killifish, Leptolucania Ommata, And Mosquitofish, Gambusia Affinis In The Okefenokee Swamp, J. Douglas Oliver

Faculty Publications and Presentations

I studied feeding dynamics of Leptolucania ommata and Gambusia affinis in the Okefenokee Swamp. Both fishes mainly ate insect larvae (such as Chironomidae) and Cladocera. Evacuation rates ranged from 0.143 (L. ommala in winter) to 0.279/hour (L. ommala in summer). Daily food consumption (dry weight) ranged from 24.2 (L. ommata in winter) to 148.3 mg/g/day (G. affinis in summer). Maximum consumption by both species was estimated at 26.31 mg/t m2 / day, in summer. These values are consistent with other observations supporting a hypothesis that invertebrate prey production is substantial in these blackwater wetlands.

Selected Studies On Certain Iron Sandwich Complexes, Mashitah M. Yusoff

Masters Theses

In these studies, the synthesis of [2.3](1,3)cyclopentadiendoparacyclophane was attempted. Like ferrocene and [2.2]paracyclophane, it is expected to exhibit transannular π-electronic interaction throughout its molecular structure and therefore, one dimensional semiconductivity.

The preparation of dialkylferrocenes had resulted in complex inseparable mixtures. Due to the fluxional nature of its intermediates, their conversion to stable Diels-Alder adducts would facilitate analysis. In this project, certain Diels-Alder adducts of bis-cyclopentadienyliron dicarbonyl intermediates were prepared for analysis.

Very few transition metal complexes of pyrrole are currently known. [2.2](2,5)Pyrroloparacyclophane was perceived to be stable especially as a part of an iron sandwich complex. The final phase …

Reductive Cleavage Of Cyclopentadienylmanganese Tricarbonyl, Mani S. Iyer

Masters Theses

Since the isolation of ferrocene in 1951, it has been shown to behave like benzene and may be acylated, sulfonated, metalated, and arylated. Like benzene, it also resists hydrogenation.

Daniel Trifan and Louis Nicholas (1957) were the first to show that ferrocene can be smoothly degraded by lithium and ethylamine. The isolation of free cyclopentadienyl ion on hydrolysis of the reaction mixture indicates that the cleavage leads initially to cyclopentadienyl lithium salt. A similar procedure applied to cyclopentadienylmanganese tricarbonyl also resulted in the formation of cyclopentadienyl lithium salt. This, when added to ferrous chloride, gave ferrocene. This technique can be …

Change Of Magnetic Properties Of Th₂Fe₁₇ Due To Interstitial Solution Of C And N, T. H. Jacobs, Gary J. Long, Oran Allan Pringle, Fernande Grandjean, K. H. Buschow

Chemistry Faculty Research & Creative Works

The changes in magnetic and crystallographic properties in the series Th₂Fe₁₇C_x and Th₂Fe ₁₇N_x have been studied. The changes in the latter series were also studied by ⁵⁷Fe Mössbauer spectroscopy. The ultimate enhancements of the saturation moments in these series are 13% and 24%, respectively. This is much larger than observed previously in the corresponding rare-earth compounds, although the Curie temperature enhancements are of comparable magnitude. In the Th-Fe-C system a novel compound of the BaCd ₁₁ type was found, having a Curie temperature around 370 K.