Digital Commons Network^™

Cutting-Edge Developments In Zinc Oxide Nanoparticles: Synthesis And Applications For Enhanced Antimicrobial And Uv Protection In Healthcare Solutions, Egwonor Loveth Irede, Raymond Femi Awoyemi, Babatunde Owolabi, Omowunmi Rebecca Aworinde, Rofiat Odunayo Kajola, Ajibola Hazeez, Ayuba Adawale Raji, Latifat Oluwatobi Ganiyu, Chimezie O. Onukwuli, Asishana Paul Onivefu, Ikhazuagbe Hilary Ifijen

Michigan Tech Publications

This paper presents a comprehensive review of recent advancements in utilizing zinc oxide nanoparticles (ZnO NPs) to enhance antimicrobial and UV protective properties in healthcare solutions. It delves into the synthesis techniques of ZnO NPs and elucidates their antimicrobial efficacy, exploring the underlying mechanisms governing their action against a spectrum of pathogens. Factors impacting the antimicrobial performance of ZnO NPs, including size, surface characteristics, and environmental variables, are extensively analyzed. Moreover, recent studies showcasing the effectiveness of ZnO NPs against diverse pathogens are critically examined, underscoring their potential utility in combatting microbial infections. The study further investigates the UV protective …

Tribocorrosion And Metal Release From Austenitic Stainless Steels 304 And 201 In Simulated Cassava Food Contact, Robert Addai, Temitope E. Olowoyo, Thalia E. Standish, Jeffrey Daniel Henderson, Ubong Eduok, Yolanda Hedberg

Chemistry Publications

Cassava is the third most significant calorie source in the tropics. Its processing has changed from traditional methods to stainless steel processing machines. This study investigated the influence of cassava on metal release from two common stainless steels, ASTM 304 and 201, with and without friction, and on tribocorrosion (multianalytically) of 304. Cassava was relatively corrosive and hindered repassivation of the surface oxide of stainless steel, but it also acted as a lubricant against mechanical friction. The combined action of friction and cassava caused a significant increase in iron, chromium, nickel, and manganese release from the stainless steels (30–35- fold …

Removing Methane Via Atmospheric Oxidation Enhancement: The Legal Framework, Romany M. Webb, Martin Lockman, Korey Silverman-Roati

Sabin Center for Climate Change Law

To achieve the Paris Agreement’s goal of limiting the increase in global average temperatures to “well below 2 degrees Celsius,” and ideally 1.5 degrees Celsius, above pre-industrial levels, global greenhouse gas (“GHG”) emissions must reach net zero in the second half of the century. The global community is not currently on track to achieve net zero emissions. In fact, with the exception of a slight dip during the Covid-19 pandemic, emissions have risen steadily in recent years. This, together with the increasingly visible impacts of climate change, has prompted growing interest in the possibility of removing GHGs directly from the …

The Chemistry Of Heavy Elements: Probing Relativistic Chemical Bonding, Barbara Maria Teixeira Costa Peluzo

Chemistry Theses and Dissertations

The bottommost part of the periodic table has a unique chemistry, where the high velocities of the inner-shell electrons prevent their description by the usual Schrödinger equation. The resulting so-called relativistic effects have numerous chemical implications, on both macro and microscale, ranging from physical properties to reactivity. However, experimental probes on these intriguing atoms are limited by their natural availability and, more importantly, the danger associated with their radioactivity. Quantum chemical calculations, on the other hand, face the challenge of far more complex algebra, which demands substantial computational resources. Relativistic Hamiltonians, which act over all electrons in the system, are …

Pressure Dependence Of The Rate Coefficient Of Ozone Using Master Equation Simulations Of Recombination Kinetics, Adil Yermek

Master's Theses (2009 -)

The ozone layer in Earth’s atmosphere is distinctive and crucial for the evolution of life. Investigating the process of ozone formation aids in comprehending the evolution of our planet’s atmosphere. The reaction that forms ozone is O + O2 → O3. One can describe ozone forming reaction approximately using Lindeman mechanism, which is very handy. However, its limitation arises from assuming a steady-state approximation, which neglects transient dynamics and complexities that could impact reaction kinetics under non-equilibrium conditions. For this reason, Master Equation simulations can be seen as an enhancement or extension of the Lindemann mechanism, providing a thorough understanding …

Theoretical And Experimental Evaluation Of The Electronic Relaxation Mechanisms Of 2-Pyrimidinone: The Primary Uva Absorbing Moiety Of The Dna And Rna (6–4) Photolesion, Sean J. Hoehn, Sarah E. Krul, Carlos E. Crespo-Hernández

Faculty Scholarship

The (6–4) photolesion is a key photodamage that occurs when two adjacent pyrimidine bases in a DNA strand bond together. To better understand how the absorption of UVB and UVA radiation by the 2-pyrimidinone moiety in a (6–4) lesion can damage DNA, it is important to study the electronic deactivation mechanism of its 2-pyrimidinone chromophore. This study employs theoretical (MS-CASPT2/cc-pVDZ level) and experimental (steady state and femtosecond broadband spectroscopic) methods to elucidate the photochemical relaxation mechanisms of 2-(1H)-pyrimidinone and 1-methyl-2-(1H)-pyrimidinone in aqueous solution (pH 7.4). In short, excitation at 320 nm leads to the population of the S1¹(ππ*) …

Poly(Thioether) Thermosets With Gated Photodegradation, Surabhi Jha

Dissertations

Photodegradable polymer networks are attractive due to the precise spatiotemporal control and large-area applicability of light. Chemical linkages that enable these polymer networks to be photo-responsive, also hinder their applicability in ambient light. Photostable and photodegradable networks have recently been reported to overcome this challenge by combining other stimuli with light for degradation. We report the design of a network that responds to a simultaneous, dual stimulus for photodegradation. We design poly(thioether acetal) networks with benzylidene acetal moieties that are known to undergo β-scission in the presence of free thiols. By blocking these free thiols with a thermally labile protecting …

Establishment Of Protocols To Measure Near-Ir(Nir) Surface-Enhanced Raman Scattering (Sers) Signals Of Nir Dye, Justin B. Ramsey

Forensic Science Master's Projects

Biomedical imaging techniques are crucial for early disease diagnostics, particularly for life-threatening conditions. However, traditional methods have inherent limitations, such as restricted spatial resolution and potential risks associated with repeated exposure to ionizing radiation. Near-infrared (NIR) surface-enhanced Raman spectroscopy (SERS) presents a promising alternative due to its minimal risks and potential for high-resolution biomedical imaging. A key challenge in NIR-SERS biomedical imaging is the development of biocompatible, nontoxic SERS substrates that can be safely introduced into the human body, targeting specific cells and tissues effectively.

NIR-SERS substrates typically consist of gold nanoparticles (AuNPs) modified with biorecognition elements and reporter molecules, …

The Analysis Of Artificially Flavored Coffees, Hannah Knights

Longwood Senior Thesis Proposal

This project aims to determine the molecular components that give artificially flavored coffees their advertised flavor. Much work has been conducted to determine various characteristics such as antioxidant properties, phenolic compounds, flavors, and aromas found in natural coffees. In addition, there is ample literature on the results of sensory tests for a variety of commercial coffees. However, not much work has been conducted to determine the compounds found in artificially flavored coffees that give rise to their distinctive aroma and flavor. This is, perhaps, due to the proprietary nature of the flavoring process employed by commercial producers. The goal of …

A Comparison Of Structure, Bonding And Non-Covalent Interactions Of Aryl Halide And Diarylhalonium Halogen-Bond Donors, Nicole Javaly, Theresa M. Mccormick, David R. Stuart

Chemistry Faculty Publications and Presentations

Halogen bonding permeates many areas of chemistry. A wide range of halogen-bond donors including neutral, cationic, monovalent, and hypervalent have been developed and studied. In this work we used density functional theory (DFT), natural bond orbital (NBO) theory, and quantum theory of atoms in molecules (QTAIM) to analyze aryl halogen-bond donors that are neutral, cationic, monovalent and hypervalent and in each series we include the halogens Cl, Br, I, and At. Within this diverse set of halogen-bond donors, we have found trends that relate halogen bond length with the van der Waals radii of the halogen and the non-covalent or …

Synthetic And Mechanistic Studies Of Ruthenium Catalyzed C–N And C–H Bond Activation Reactions, Krishna Prasad Gnyawali

Dissertations (1934 -)

Transition metal catalyzed C–H and C–N functionalization methods have emerged as an innovative tool for designing efficient synthesis of fine chemicals by using readily available starting materials without the formation of wasteful byproducts. One of the paramount challenges in C–H functionalization research field has been to promote regio- and stereoselective C–H bond activation on saturated hydrocarbon substrates. While much progress has been achieved on promoting selective sp2 C–H activation reactions on unsaturated hydrocarbon substrates in the past decades, catalytic sp3 C–H bond and functionalization methods on saturates hydrocarbon substrates remain as the frontier topic of C–H functionalization research. The well-defined …

Precision Deuteration And Hydrofunctionalization Of Aryl Alkynes And Alkenes, Samantha E. Sloane

Dissertations (1934 -)

The similar nature of the hydrogen atom to its isotope, deuterium, allows for the simple exchange of hydrogen atoms for deuterium atoms in drug molecules to alter the absorption, distribution, metabolism, and excretion properties. Installing the deuterium functionality into a specific site in the molecule is essential. Selective hydrofunctionalization reactions of alkynes and alkenes using the highly reactive catalytic Cu–H species have been well developed, and synthetic organic chemistry methods to selectively incorporate one or two deuterium atoms into the benzylic site of organic compounds, a key metabolic position, are elusive. A Cu–H catalytic approach offers selectivity and reactivity to …

Visible-Light-Driven Reactions From A Six-Coordinate Nickel(Ii) Complex, Lakmini Lochana Edirisinghe Arachchilage

Master's Theses (2009 -)

Transition metal complexes capable of absorbing visible light can be excited upon light irradiation and facilitate chemical bond formation and cleavage. In this approach, a transition metal-substrate complex is formed in the ground state, followed by photoinduced substrate activation via an inner-sphere mechanism. Unlike well-established photoredox catalysis that does not involve substrate binding, the ground-state interaction here is important for substrate activation. Among the 3d transition metals, nickel is considered a cost-effective alternative to Ru- and Ir-based photocatalysts due to its ability to accommodate different oxidation states. However, the application of Ni photocatalysis in synthesis had remained largely underexplored. In …

Beta Lactams Anticancer Effect On Human Breast Cancer, Mcf-7 Cell Line, Ailis A. Abalos

Theses and Dissertations

Cancer is a disease where abnormal cells replicate uncontrollably in that same region and/or other parts of the body. One in eight women are diagnosed with breast cancer, thus making it the second leading cause of cancer-related deaths in the U.S. Chemotherapy, is the utilization of medicinal compounds (drugs) to treat cancer. While most are nonspecific and target both cancer and healthy cells, more emphasis is on developing drugs with specificity (only target tumor cells) and low toxicity. Interestingly, Beta Lactams (β-lactams) have emerged as a class of compounds that are effective in the treatment of various cancers. The benefits …

Modulating Covalency In A Ni-H-Al Subunit: Synthesis And Characterization Of A Series Of Electronically Differentiating Ni-Al Heterometallics, Aleida Guadalupe Gonzalez

Theses and Dissertations

Metal hydrides are moieties that are present in a variety of applications as intermediates in catalysis. Despite the vast utilization of metal hydrides, monometallic metal hydride complexes bear limitations in inert-bond activation and functionalization. A strategy to overcome these limitations and access more challenging reactivity is utilizing metal-metal cooperativity on a single platform to further activate hydride moieties. Although incorporating multiple metal centers on a single ligand scaffold has been realized, access to heterometallic hydride-bearing complexes is a greater synthetic challenge. Given the limitations in accessing such systems, the reactivity and electronic structure of heterometallic hydrides are seldom studied. Recently, …

A Deep Dive Into The Conformations Of Fluorine-Substituted Benzoic Acids And Ethanesulfonic Acid And Spectroscopic Characterizations Of Their Hydrogen-Bonded Complexes, Alitza Gianina Gracia

Theses and Dissertations

Microwave spectroscopy is a technique used to study the molecular structures of a wide range of systems. The first part of the thesis focuses on fluorinated benzoic acids and complexes they form through hydrogen bonding to explore the fluorination effect, while the last part shifts to ethanesulfonic acid and its interaction with water. The study of 3,4,5-triflurorbenzoic acid with formic acid offers insight on the proton tunneling process, which is a process that occurs in many chemical and biological processes. Microwave spectroscopy can help provide dynamic information for the better understanding of this fundamental process. Solvation is another topic that …

Response To Replication Stress And Maintenance Of Genome Stability By Wrn, The Werner Syndrome Protein, David K. Orren, Amrita Machwe

Markey Cancer Center Faculty Publications

Werner syndrome (WS) is an autosomal recessive disease caused by loss of function of WRN. WS is a segmental progeroid disease and shows early onset or increased frequency of many characteristics of normal aging. WRN possesses helicase, annealing, strand exchange, and exonuclease activities and acts on a variety of DNA substrates, even complex replication and re- combination intermediates. Here, we review the genetics, biochemistry, and probably physiological functions of the WRN protein. Although its precise role is unclear, evidence suggests WRN plays a role in pathways that respond to replication stress and maintain genome stability particularly in telomeric regions.

Preferential Crystallization Of (±)-Pinenyllithium·Tmeda, Alexander N. Erickson, Curtis Haltiwanger, Masoumeh Rahim, Charles M. Garner

Chemistry Faculty Research and Publications

(±)-Pinenyllithium·TMEDA or (tetra­methyl­ethylenedi­amine-κ²N,N′)(η³-6,6-di­methyl-2-methylenebi­cyclo­[3.1.1]hept­yl)lithium, [Li(C₁₀H₁₅)(C₆H₁₆N₂)], is readily pre­pared from β-pinene, butyl­lithium and TMEDA, and the racemic material pre­fer­entially crystallizes even from 96:4 (92% ee) mixtures of (−)- and (+)-β-pinene, respectively. The structure is monomeric, with the geminal-dimethyl bridge of the bicyclic structure shielding one face of the allyl system, restricting the lithium to the opposite face and preventing the Li–all­yl–Li aggregation observed with some other allyl­lithium systems. The symmetry of the allyl system, bond lengths, bond angles and out-of-plane deviations …

Improvement Of Triboelectric Nanogenerator By As-Prepared Lithium Niobate For Energy Harvesting And Sensing Applications, Jahid Inam Chowdhury

Theses and Dissertations

One readily available green energy source that is used to address the small-scale energy requirement is mechanical energy. To harvest mechanical energy, supply energy for upcoming electronic devices, and enable flexible health monitoring, triboelectric nanogenerators composed of perovskite and lightweight polymers have garnered significant interest. A material named lithium niobate (LiNbO3) was produced in two different phases and used to make a flexible triboelectric nanogenerator (TENG). Furthermore, lightweight polydimethylsiloxane (PDMS), Polyvinyl Alcohol (PVA), and LiNbO3 nanoparticles were used to produce a small triboelectric nanogenerator that successfully converts mechanical energy into electricity. LiNbO3 nanoparticles were first created and loaded into polydimethylsiloxane …

Use Of Cobalt(Iii) And Copper(Ii) Complexes With Thiosemicarbazone Ligands And Berberine Chloride As Anti-Cancer Agents Against Triple Negative Breast Cancer, Duaa Rashad Alajroush

Chemistry & Biochemistry Theses & Dissertations

It is a worrying fact that chemotherapeutic options for treating triple negative breast cancer are limited in nature, including the use of inorganic pharmaceuticals such as cisplatin and its analogues. It has been reported that cisplatin has undesirable side effects when used as a chemotherapeutic drug. As such, there is a need to use non-platinum-containing complexes as chemotherapeutic agents. Based on that issue, it has been reported by our research group and others that cobalt- and copper-containing complexes have shown great potential in anti-cancer properties when compared to cisplatin. In this research project, the cytotoxic effects of the following complexes: …

Study Of Atypical Alarmone Synthesis In Clostridioides Difficile, Declan Nathaniel Butler

Chemistry & Biochemistry Theses & Dissertations

Clostridioides difficile is a Gram-positive anaerobic bacterium that causes infections in humans that costs healthcare systems billions per year. C. difficile infection has high rates of recurrence due to multiple antibiotic resistance. When bacteria are in stressful environments, they produce hyperphosphorylated guanosine ribonucleotide signaling molecules called alarmones. The accumulation of alarmones activates the stringent response (SR), in which bacterial cells induce transcription of stress survival genes to delay growth and replication. The C. difficile SR is regulated by enzymatic activity of a bifunctional synthetase/hydrolase, RelA/SpoT homolog (RSH), and a monofunctional small alarmone synthetase (RelQ). Additionally, the SR is potentially regulated …

Synthesis And Characterization Of Cobalt-Based Complexes And Metal-Organic Frameworks For Hydrogen Evolution Catalysis Under Acidic Conditions, Elizabeth A. Tonsel-White

Chemistry & Biochemistry Theses & Dissertations

The synthesis of two cobalt(II) complexes: the novel complex [Co(phen)₃]₂[Co(pytc)₂]•16.25H₂O 1 and the previously reported [Co(bpy)₃](OH)₂•7H₂O 2, along with a cobalt(II/III)-containing MOF, Co-PPF2, previously reported as well, was achieved. These complexes were characterized by elemental analysis, X-ray crystallography, microscopy, conductivity measurements, TGA, SEM, CV , and UV-visible , and FTIR spectroscopies. From the CV scan rates, diffusion coefficients for complexes 1 and 2 were determined to be 1.0 × 10⁻⁶ cm² s⁻¹ and 4.8 × 10⁻⁷ cm² s⁻¹. Electrocatalytic studies with complexes 1 and 2 revealed insights into the mechanistic aspects of …

Exploring The Effects Of An Alfalfa Leaf-Derived Adsorbent On Microbial Community, Ileal Morphology, Barrier Function, And Immunity In Turkey Poults During Chronic Aflatoxin B1 Exposure, Maria De Jesus Nava-Ramirez, Jing Liu, Juan Omar Hernandez-Ramirez, Xochitl Hernandez-Velasco, Juan D. Latorre, Alma Vazquez-Duran, Guolong Zhang, Roberto Señas-Cuesta, Sergio Gomez-Rosales, Andressa Stein, Billy M. Hargis, Guillermo Tellez-Isaias, Abraham Mendez-Albores, Jesus A. Maguey-Gonzalez

Poultry Science Faculty Publications and Presentations

This article follows-up on our recently published work, which evaluated the impact of the addition of an alfalfa leaf-derived adsorbent in the aflatoxin B₁ (AFB₁)-contaminated diet in regard to the production parameters, blood cell count, serum biochemistry, liver enzymes, and liver histology of turkey poults. This paper presents complementary results on microbial community, ileal morphology, barrier function, and immunity. For this purpose, 350 1-day-old female turkey poults were randomly distributed into five groups: (1) Control, AFB₁-free diet; (2) AF, AFB₁-contaminated diet at 250 ng/g; (3) alfalfa, AFB₁-free diet + 0.5% ( …

Carbon-Based Nanocomposite Membranes For Membrane Distillation: Progress, Problems And Future Prospects, Chhabilal Regmi, Yuwaraj K. Kshetri, S. Ranil Wickramasinghe

Chemical Engineering Faculty Publications and Presentations

The development of an ideal membrane for membrane distillation (MD) is of the utmost importance. Enhancing the efficiency of MD by adding nanoparticles to or onto a membrane’s surface has drawn considerable attention from the scientific community. It is crucial to thoroughly examine state-of-the-art nanomaterials-enabled MD membranes with desirable properties, as they greatly enhance the efficiency and reliability of the MD process. This, in turn, opens up opportunities for achieving a sustainable water–energy–environment nexus. By introducing carbon-based nanomaterials into the membrane’s structure, the membrane gains excellent separation abilities, resistance to various feed waters, and a longer lifespan. Additionally, the use …

Pseudo-Siamese Network Combined With Label-Free Raman Spectroscopy For The Quantification Of Mixed Trace Amounts Of Antibiotics In Human Milk: A Feasibility Study, Jing Yi Mou, Muhammad Usman, Jia Wei Tang, Quan Yuan, Zhang Wen Ma, Xin Ru Wen, Zhao Liu, Liang Wang

Research outputs 2022 to 2026

The utilization of antibiotics is prevalent among lactating mothers. Hence, the rapid determination of trace amounts of antibiotics in human milk is crucial for ensuring the healthy development of infants. In this study, we constructed a human milk system containing residual doxycycline (DXC) and/or tetracycline (TC). Machine learning models and clustering algorithms were applied to classify and predict deficient concentrations of single and mixed antibiotics via label-free SERS spectra. The experimental results demonstrate that the CNN model has a recognition accuracy of 98.85% across optimal hyperparameter combinations. Furthermore, we employed Independent Component Analysis (ICA) and the pseudo-Siamese Convolutional Neural Network …

Problem-Based Learning Model Assisted With A Humanistic-Merdeka Belajar Approach Improved Biology Students’ Learning Ownership, Dita Tri Mulyani, Dwi Listyorini

Jurnal Pendidikan Sains

Fostering ownership over learning constitutes a pivotal aspect for students to cultivate a comprehensive understanding, employ cognitive strategies effectively, hone essential skills, and refine learning methodologies. Preliminary studies have underscored a prevalent deficiency in the learning ownership among biology students, categorizing it as notably low. The purpose of this study is to ascertain the effect of the Project Based Learning (PBL) model assisted with a Humanistic-Merdeka Belajar (HMB) approach, in nurturing students’ ownership of their learning endeavors. Employing a quasi-experimental design, the research adopts a pre-test post-test non-equivalent control group configuration, administered both prior to and following the intervention within …

Asistensi Mengajar (Am) Program In Merdeka Belajar Kampus Merdeka (Mbkm) As A Pedagogical Mastery Accelerator For Vocational High School Teacher Candidates, Muhammad Idris Effendi, Yoto Yoto, Widiyanti Widiyanti

Jurnal Pendidikan Sains

This research delves into the issue of inadequate pedagogical proficiency among vocational high school (SMK) teacher candidates, primarily stemming from the constrained timeframe of educational internships, typically spanning a mere two months. As an option, this study suggests the implementation of an Asistensi Mengajar (AM) initiative within the framework of the Merdeka Belajar Kampus Merdeka Program (MBKM), extending over a semester’s duration. Employing a literature review methodology, the research highlights that the brevity of conventional two-month internships poses a significant impediment to the development of pedagogical acumen among SMK teacher candidates. The proposed option entails an AM program under MBKM, …

Identify Resources Activated By Students When Solving Straight Motion Kinematics Problems With Different Representation, Nur Utami Amaliah, Sutopo Sutopo, Eny Latifah, Hutkemri Zulnaidi

Jurnal Pendidikan Sains

This research aims to explore kinematic resources in linear kinematics by utilizing different forms of problem representation. The study was conducted by administering 15 reasoned multiple-choice questions to 146 high school students from three districts in Central Sulawesi, Indonesia. The research instrument covers topics in linear kinematics, including distance, displacement, speed, and velocity, presented through diagrammatic representations, graphs, and mathematical equations. The students’ written answers were analyzed both quantitatively and qualitatively. Quantitative analysis was conducted to determine descriptive statistics from the data, while qualitative analysis identified the resources students activated when solving these questions. The findings reveal that the various …

Analysis Of Genetic Engineering Material Misconceptions In Biology Textbooks For Ninth-Grade In Yogyakarta, Silfi Pratiwi, Purwanti Pratiwi Purbosari, Oktira Roka Aji, Rinaldi Rizal Putra

Jurnal Pendidikan Sains

Textbooks play a pivotal role in the pedagogical landscape, serving as indispensable aids for learning, founts of knowledge, and platforms for practical application. This study endeavors to scrutinize the content of textbooks, specifically focusing on ninth-grade biology textbooks within the educational milieu of Yogyakarta City, and to juxtapose these contents with existing literature to discern potential misconceptions. Employing a descriptive qualitative approach, the investigation centers on printed biology textbooks utilized in ninth-grade curricula across junior high schools in Yogyakarta. The selection of textbooks for analysis was based on their prevalence as instructional materials, as determined through survey data. Genetic engineering …

Students’ Science Literacy On Discussion-Based Contextual Approaches Of Human Reproductive System Learning, Yeni Mulyaningsih, Chaerul Rochman, Adam Malik, Tri Wahyu Agustina, Yulia Sukmawardani, Nur Hamidah, Nurul Annisa

Jurnal Pendidikan Sains

The aim of this study was to enhance students’ science literacy through a discussion-based contextual approach in the learning of the human reproductive system. This quantitative research employed an experimental method with a quasi-experimental posttest-only design. The study involved 60 students, with 30 students each in class XI MIPA 4 serving as the experimental group and class XI MIPA 5 serving as the control group. The control group received instruction through a conceptual approach-based lecture method, whereas the experimental group engaged in learning through a contextual approach-based discussion method. The research instrument comprised 10 open-ended science literacy questions pertaining to …