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Biological Interaction Of Mn-Based Nanomaterials With Capsicum Annuum L. Under Salinity Stress, Yuqing Ye Aug 2022

Biological Interaction Of Mn-Based Nanomaterials With Capsicum Annuum L. Under Salinity Stress, Yuqing Ye

Open Access Theses & Dissertations

The foliar application of nanomaterials as a method to overcome abiotic stress is relatively new compared to their use as fertilizers or pesticides in agricultural practice. It is also vital to understand the interface between leaf and nanomaterials, as nanomaterials might translocate into crop edible tissues, thus, causing potential toxicity in living beings during food consumption. In this dissertation, salt-stressed Capsicum annuum L was exposed to manganese (Mn) -based nanomaterials (Mn2O3 nanoparticles (MnNPs) and graphene quantum dots doped with Mn (GQD-Mn)) at different stages (seed germination and full life cycle). The dissertation focuses on investigating the mechanism of how Mn-based …

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Alkylthiocarbamate Metal Complexes With Antiproliferation Activity., Kritika Bajaj Aug 2022

Alkylthiocarbamate Metal Complexes With Antiproliferation Activity., Kritika Bajaj

Electronic Theses and Dissertations

Bis(thiosemicarbazones) (BTSCs) and their metal complexes have been extensively studied for various applications including catalysis for hydrogen evolution reactions, treatment of neurodegenerative diseases, hypoxia imaging, and a wide range of pharmacological purposes including antitumor, antiviral, antibacterial, and antifungal agents. The paramount property that makes BTSC metal complexes interesting for their use in these applications is the reduction potential of the coordinated metal, which can be selectively tuned by varying the substituents present on the ligand framework. Although the BTSCs have shown a great deal of success as potential cancer therapeutic agents, they lack selectivity towards cancer cells. For this matter, …

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Properties And Behavior Of Tricarbonyl Manganese Scorpionate Complexes, Billy Shone Jul 2022

Properties And Behavior Of Tricarbonyl Manganese Scorpionate Complexes, Billy Shone

Master's Theses (2009 -)

Manganese tricarbonyl complexes have widely been studied for their ability to release carbon monoxide (CO) in the presence of light for potential applications in cancer therapy and in catalysis. Most studies focus on the kinetics and quantity of CO release and the overall impact on cell death. Far fewer studies identify the manganese intermediates and final products after decarbonylation. Identification of such products would be critical to improving reactivity and understanding potential (undesirable) side effects in future applications employing these CO releasing molecules (CORMs). Recently, nitrogen confused C-scorpionate ligands were introduced by the Gardinier group where a pyrazolyl ring is …

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Synthesis And Antibacterial Testing Of Novel Thiosulfonate Compounds, Lindsay I. Blume Jun 2022

Synthesis And Antibacterial Testing Of Novel Thiosulfonate Compounds, Lindsay I. Blume

USF Tampa Graduate Theses and Dissertations

Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Staphylococcus aureus (VRSA) are increasing concerns in the medical community. In addition to the threat of Staphylococcus aureus, the increase in number and severity of infections caused by other antibiotic-resistant bacteria is problematic to human health, particularly among the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter). The antibiotics like the penicillins and cephalosporins used commonly to treat MRSA, VRSA, and ESKAPE pathogens have continued to decline in the effectiveness. Our laboratory has developed a number of new families of anti-MRSA and anti-VRSA antibacterial compounds. In this thesis, …

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Noncanonical Amino Acid Mutagenesis In Response To Recoding Signal-Enhanced Quadruplet Codons, Yan Chen, Xinyuan He, University Of Nebraska-Lincoln, Kun Liu, Tian Gao, Wei Niu, Jiantao Guo Jun 2022

Noncanonical Amino Acid Mutagenesis In Response To Recoding Signal-Enhanced Quadruplet Codons, Yan Chen, Xinyuan He, University Of Nebraska-Lincoln, Kun Liu, Tian Gao, Wei Niu, Jiantao Guo

Department of Chemistry: Faculty Publications

While amber suppression is the most common approach to introduce noncanonical amino acids into proteins in live cells, quadruplet codon decoding has potential to enable a greatly expanded genetic code with up to 256 new codons for protein biosynthesis. Since triplet codons are the predominant form of genetic code in nature, quadruplet codon decoding often displays limited efficiency. In this work, we exploited a new approach to significantly improve quadruplet UAGN and AGGN (N = A, U, G, C) codon decoding efficiency by using recoding signals imbedded in mRNA. With representative recoding signals, the expression level of mutant proteins containing …

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Signaling Studies Of Protein-Peptide Binding On Microelectrode Arrays, Kendra White May 2022

Signaling Studies Of Protein-Peptide Binding On Microelectrode Arrays, Kendra White

Arts & Sciences Graduate Student Theses and Dissertations

Electrochemical methods provide unique opportunities for non-traditional synthesis and analytical studies. Using electrochemistry, we are able to synthesize individually addressable molecular surfaces on microelectrode arrays and then utilize the surface to probe interactions between receptors and their biological targets. To date, cyclic voltammetry signals can be used to detect biological interactions between proteins and small molecules on the surface of an array, however those signals are often amplified by the presence of a large number of small molecules by any given electrode. This amplification makes it difficult to obtain binding data using the arrays that can be compared with existing …

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Synthesis And Function Of Ion-Selective Single-Molecule Fluorescent Imaging Molecules And Materials, Alena A. Denisenko May 2022

Synthesis And Function Of Ion-Selective Single-Molecule Fluorescent Imaging Molecules And Materials, Alena A. Denisenko

Chemistry & Biochemistry Dissertations - Archive

Neutrinos are fermion subatomic particles that interact only by weak subatomic forces—making them almost impossible to detect. However, they can be produced in specific radioactive decay reactions, such as beta decay. We hypothesize the detection of double beta decay through the tagging of the Ba2+ daughter ion from 136Xe (136Xe —136Ba2+ + 2ν + 2β). The proposal is to develop single-molecule fluorescence imaging (SFMI) technology to investigate gaseous chemical constituents in high-pressure gases (ca. 30-150 psi) at gas-solid interfaces. To achieve proper SMFI, heterogenous self-assembled monolayers (SAMs) containing fluorophores that operate under dry conditions and are turned on when a …

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Synthesis And Characterization Of Organometallic Complexes For Biological Imaging, Emily Stumbo May 2022

Synthesis And Characterization Of Organometallic Complexes For Biological Imaging, Emily Stumbo

Honors Theses

Luminescent transition metal complexes are of interest for biological imaging applications due to their unique photophysical properties including large Stokes shift, long fluorescence lifetimes, and tunable emission. Altering ligand structure is a useful way to manipulate the charge transfer (CT) and mixed character states that give these complexes their photophysical properties. A series of organometallic complexes containing ligands with externally facing nitrogens has been prepared and synthesized. Complexes of the form Pt (II)(tbbpy)(C2-py), Re(CO)3(N^N), Ir(C^N)2(N^N), and Ir(C^N)2(N^O) have been studied for their potential application as biological imaging agents. The goal of this project is to compare the properties of these …

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Computer Simulations And Network-Based Profiling Of Binding And Allosteric Interactions Of Sars-Cov-2 Spike Variant Complexes And The Host Receptor: Dissecting The Mechanistic Effects Of The Delta And Omicron Mutations, Gennady M. Verkhivker, Steve Agajanian, Ryan Kassab, Keerthi Krishnan Apr 2022

Computer Simulations And Network-Based Profiling Of Binding And Allosteric Interactions Of Sars-Cov-2 Spike Variant Complexes And The Host Receptor: Dissecting The Mechanistic Effects Of The Delta And Omicron Mutations, Gennady M. Verkhivker, Steve Agajanian, Ryan Kassab, Keerthi Krishnan

Mathematics, Physics, and Computer Science Faculty Articles and Research

In this study, we combine all-atom MD simulations and comprehensive mutational scanning of S-RBD complexes with the angiotensin-converting enzyme 2 (ACE2) host receptor in the native form as well as the S-RBD Delta and Omicron variants to (a) examine the differences in the dynamic signatures of the S-RBD complexes and (b) identify the critical binding hotspots and sensitivity of the mutational positions. We also examined the differences in allosteric interactions and communications in the S-RBD complexes for the Delta and Omicron variants. Through the perturbation-based scanning of the allosteric propensities of the SARS-CoV-2 S-RBD residues and dynamics-based network centrality and …

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Exploring The Structure And Activity Of Metallo-Tetracyclines, Shahedul Islam Mar 2022

Exploring The Structure And Activity Of Metallo-Tetracyclines, Shahedul Islam

USF Tampa Graduate Theses and Dissertations

Copper as a key component of electron transport chain of eukaryotes is an essential transition metal ion. Copper homeostasis in mammals complex and tightly regulated. Its strong reactivity together with binding with biologically important chemicals can have important repercussions in human health and overall environment. Tetracycline as one of most abundantly used antibiotic of the world has become abundant in the environment as well. Herein in this dissertation we take the journey to explore the complexation of tetracycline with metals in the environment and the intricate interaction between copper and tetracycline by investigating their oxidative behavior once they are complexed. …

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Natural Phaeosphaeride A Derivatives Overcome Drug Resistance Of Tumor Cells And Modulate Signaling Pathways, Victoria Abzianidze, Natalia Moiseeva, Diana Suponina, Sofya Zakharenkova, Nadezhda Rogovskaya, Lidia Laletina, Alvin A. Holder, Denis Krivorotov, Alexander Bogachenkov, Alexander Garabadzhiu, Anton Ukolov, Vyacheslav Kosorukov Mar 2022

Natural Phaeosphaeride A Derivatives Overcome Drug Resistance Of Tumor Cells And Modulate Signaling Pathways, Victoria Abzianidze, Natalia Moiseeva, Diana Suponina, Sofya Zakharenkova, Nadezhda Rogovskaya, Lidia Laletina, Alvin A. Holder, Denis Krivorotov, Alexander Bogachenkov, Alexander Garabadzhiu, Anton Ukolov, Vyacheslav Kosorukov

Chemistry & Biochemistry Faculty Publications

n the present study, natural phaeosphaeride A (PPA) derivatives are synthesized. Anti-tumor studies are carried out on the PC3, K562, HCT-116, THP-1, MCF-7, A549, NCI-H929, Jurkat, and RPMI8226 tumor cell lines, and on the human embryonic kidney (HEK293) cell line. All the compounds synthesized turned out to have better efficacy than PPA towards the tumor cell lines listed. Among them, three compounds exhibited an ability to overcome the drug resistance of tumor cells associated with the overexpression of the P-glycoprotein by modulating the work of this transporter. Luminex xMAP technology was used to assess the effect of five synthesized compounds …

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Catalytic Enantioselective Birch–Heck Sequence For The Synthesis Of Phenanthridinone Derivatives With An All-Carbon Quaternary Stereocenter, Mary Elizabeth Sexton, William Paul Malachowski, Glenn P. A. Yap, Diana Rachii, Greg Feldman, Andrew T. Krasley, Zhilin Chen, My Anh Tran, Kalyn Wiley, Alexandra Matei, Samantha Petersen, Sabrina Tran Tien Jan 2022

Catalytic Enantioselective Birch–Heck Sequence For The Synthesis Of Phenanthridinone Derivatives With An All-Carbon Quaternary Stereocenter, Mary Elizabeth Sexton, William Paul Malachowski, Glenn P. A. Yap, Diana Rachii, Greg Feldman, Andrew T. Krasley, Zhilin Chen, My Anh Tran, Kalyn Wiley, Alexandra Matei, Samantha Petersen, Sabrina Tran Tien

Chemistry Faculty Research and Scholarship

Novel phenanthridinone analogues with an all-carbon quaternary stereocenter have been enantioselectively synthesized using the Birch–Heck sequence. Flat phenanthridinone structures have extensive bioactivity but consequently also suffer from poor therapeutic selectivity. The addition of a quaternary center to the phenanthridinone skeleton has the potential to generate more complex analogues with improved selectivity. Unfortunately, no general synthetic pathway to such derivatives exists. Herein we report a four-step process that transforms inexpensive benzoic acid into 22 different quaternary carbon-containing phenanthridinone analogues with a variety of substituents on all three rings: alkyl groups at the quaternary center; methyl, methoxymethyl, or para-methoxybenzyl on the …

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Development Of New Photochemical Tools For Applications In Cancer Research And Enzymatic Signaling, Nicholas Paul Toupin Jan 2022

Development Of New Photochemical Tools For Applications In Cancer Research And Enzymatic Signaling, Nicholas Paul Toupin

Wayne State University Dissertations

Photochemical tools empower researchers to act with precision and control over the cellular environment through production of reactive oxygen species (ROS), targeted drug delivery, and modulation of enzymatic activity. These molecular tools use light as a readily available, biorthogonal reagent for initiating advantageous light-activated reactions. Using light in this way allows researchers to affect the cellular environment in specific ways with high spatio-temporal control. This dissertation focuses on expanding the applications of, mostly Ru(II)-based, photoactivated chemicals. Complexes of this type are able to act as photosensitizers in photodynamic therapy (PDT), as photocages (PCT), or as dual-action compounds capable of both …

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Clerodendrum Serratum Extract Attenuates Production Of Inflammatory Mediators Inovalbumin-Induced Asthma In Rats, Poonam Arora, Shahid Hamid Ansari, Lalit Mohan Nainwal Jan 2022

Clerodendrum Serratum Extract Attenuates Production Of Inflammatory Mediators Inovalbumin-Induced Asthma In Rats, Poonam Arora, Shahid Hamid Ansari, Lalit Mohan Nainwal

Turkish Journal of Chemistry

In the present study, ethanolic extract of Clerodendrum serratum roots was investigated for its potential to reverse some features of bronchial asthma in ovalbumin-induced murine model of asthma. Clerodendrum serratum commonly called bharangi, (family Solanaceae) is a well-known anti-allergic drug in Asian folk system of medicines. In the present work, pharmacological studies are done to provide scientific evidence for therapeutic potential of plant in allergic asthma. Asthma was induced in experimental rats with allergen suspension of ovalbumin and aluminum hydroxide followed by treatment with dexamethasone (2.5 mg/kg, po) or C. serratum root extract (0.53 and 5.3 mg/kg, b. w., po). …

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Fluorometric Determination Of Ornidazole By Using Bsa Coated Copper Nanoclusters As A Novel Turn Off Sensor, Mehmetcan Bi̇lkay, Hayri̇ye Eda Şatana Kara Jan 2022

Fluorometric Determination Of Ornidazole By Using Bsa Coated Copper Nanoclusters As A Novel Turn Off Sensor, Mehmetcan Bi̇lkay, Hayri̇ye Eda Şatana Kara

Turkish Journal of Chemistry

A fluorescent probe based on bovine serum albumin stabilized copper nanoclusters (BSA-CuNCs) was developed for the selective and sensitive determination of ornidazole (ORN). The nanoclusters were synthesized via a one-pot hydrothermal process in basic media. The synthesized and characterized BSA-CuNCs have less than 3 nm particle size and exhibited blue emission at 405 nm when excited at 325 nm. Synthesized and characterized nanoclusters were successfully applied as a turn-off fluorescent probe for the determination of ORN in pharmaceutical dosage forms. The quenching mechanism created an was inner filter effect (IFE). The method was linear in the concentration range of 0.52-13.56 …

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Synthesis, Antioxidant Activity, Molecular Docking And Adme Studies Of Novel Pyrrolebenzimidazolederivatives, Fi̇kri̇ye Zengi̇n Karadayi, Rahman Başaran, Mehmet Murat Kişla, Bi̇nay Eke, Zeynep Alagöz Jan 2022

Synthesis, Antioxidant Activity, Molecular Docking And Adme Studies Of Novel Pyrrolebenzimidazolederivatives, Fi̇kri̇ye Zengi̇n Karadayi, Rahman Başaran, Mehmet Murat Kişla, Bi̇nay Eke, Zeynep Alagöz

Turkish Journal of Chemistry

Several 5-(alkylsulfonyl)-1-substituted-2-(1H-pyrrol-2-yl)-1H-benzo[d]imidazole derivatives were synthesized and their antioxidant activities were investigated using lipid peroxidation (LPO) and 7-ethoxyresorufin O-deethylase (EROD) assays. Docking analysis with Human NAD[P]H-Quinone oxidoreductase 1 (NQO1) was also performed to gather thorough information about these compounds that have antioxidant activities. Moreover, their molecular descriptors and ADME properties were calculated using the SwissADME online program. As a result, most of our compounds possessed better affinity and created ample interactions with NQO1. The most potent compound 5j had LP inhibition value of 3.73 nmol/mg/min. Other compounds exhibited moderate activity on LP levels comparing to standard butylated hydroxy toluene (BHT). However, …

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Synthesis, Characterization, And Antimicrobial Evaluation Of Polyvinylalcohol-Osthol Composite Films, Ishrat Gowsia, Feroz Mir, Javid Banday Jan 2022

Synthesis, Characterization, And Antimicrobial Evaluation Of Polyvinylalcohol-Osthol Composite Films, Ishrat Gowsia, Feroz Mir, Javid Banday

Turkish Journal of Chemistry

Although poly-vinyl alcohol (PVA) has certain mechanical drawbacks such as a weak barrier, it has widely been used in food packaging over the last many years. To increase the suitability of PVA (C2H4O)n and render it ideal for food packaging, a diversity of studies have already been carried out. In the below-mentioned script, we, for the first time, report the use of natural product osthol in making a new composite with PVA for enhancing thermal, physicochemical, and antimicrobial properties. The significant aim of the report is the insertion of osthol (C15H16O3 …

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Encapsulation Of Halocadmate Anion Via Hydrogen Bonding: Synthesis And Characterization Of Bis(Diethylenetriamine)Cobalt(Iii) Complex Containing Hexabromocadmate Anion, Vinit Prakash, Ritu Bala, Amrinder Kaur, Daron E. Janzen Jan 2022

Encapsulation Of Halocadmate Anion Via Hydrogen Bonding: Synthesis And Characterization Of Bis(Diethylenetriamine)Cobalt(Iii) Complex Containing Hexabromocadmate Anion, Vinit Prakash, Ritu Bala, Amrinder Kaur, Daron E. Janzen

Turkish Journal of Chemistry

In this work, the encapsulation of [CdBr6]4- by six cations, [Co(dien)2]3+ has been described with the help of single crystal X-ray structural study in the complex, mer-[Co(dien)2]2[CdBr6]Br2. The complex anion, [CdBr6]4- has been obtained through solution method while attempting to synthesize complex dianion, [CdBr4]2-. This newly synthesized complex has been initially characterized by elemental analyses and spectroscopic studies (IR, NMR and UV-Visible). IR and NMR studies have been used for the isomeric identification of [Co(dien)2]3+ …

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Expression And Purification Of A Cleavable Recombinant Fortilin From Escherichia Coli For Structure Activity Studies, Maranda S. Cantrell, Jackson D. Wall, Xinzhu Pu, Matthew Turner, Luke Woodbury, Owen M. Mcdougal, Lisa R. Warner Jan 2022

Expression And Purification Of A Cleavable Recombinant Fortilin From Escherichia Coli For Structure Activity Studies, Maranda S. Cantrell, Jackson D. Wall, Xinzhu Pu, Matthew Turner, Luke Woodbury, Owen M. Mcdougal, Lisa R. Warner

Chemistry and Biochemistry Faculty Publications and Presentations

Complications related to atherosclerosis account for approximately 1 in 4 deaths in the United States and treatment has focused on lowering serum LDL-cholesterol levels with statins. However, approximately 50% of those diagnosed with atherosclerosis have blood cholesterol levels within normal parameters. Human fortilin is an anti-apoptotic protein and a factor in macrophage-mediated atherosclerosis and is hypothesized to protect inflammatory macrophages from apoptosis, leading to subsequent cardiac pathogenesis. Fortilin is unique because it provides a novel drug target for atherosclerosis that goes beyond lowering cholesterol and utilization of a solution nuclear magnetic resonance (NMR) spectroscopy, structure-based drug discovery approach requires milligram …

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The Photo-Transformation Of Free Methionine In The Presence Of Surrogate And Standard Isolate Dissolved Organic Matter Under Sunlit Irradiation, Benjamin J. Mohrhardt Jan 2022

The Photo-Transformation Of Free Methionine In The Presence Of Surrogate And Standard Isolate Dissolved Organic Matter Under Sunlit Irradiation, Benjamin J. Mohrhardt

Dissertations, Master's Theses and Master's Reports

Sulfur (S)-containing amino acids are key sources of carbon, nitrogen, and sulfur involved in protein synthesis, protein function, and providing energy for microbial growth. Dissolved free and combined methionine is one of two S-containing amino acids incorporated into proteins and has been attributed to their stability and function. The oxidation of methionine has received considerable attention given its ubiquitous presence in most biological systems and has been associated with losses in protein function and pathological disorders. In natural waters, methionine is rapidly and selectively taken up by microorganisms to achieve cellular requirements of carbon, nitrogen, and sulfur. The abiotic transformation …

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Gene Vectors With Fluorescence Tracking Capabilities, Sophia Despina Angelopoulos Jan 2022

Gene Vectors With Fluorescence Tracking Capabilities, Sophia Despina Angelopoulos

Browse all Theses and Dissertations

This project focuses on the optimization of benzothiazole-based chromophores to utilize them as fluorescent tags functionalized onto stimuli-responsive, or “smart,” polymers as non-viral gene delivery vectors for gene therapy applications. Blue-fluorescent emissive chromophores will allow tracking capabilities for the transfection pathway of the vector to be monitored as it delivers the DNA payload within intercellular space. The most appropriate chromophore must be covalently bound to a free amine within the vector, which is accomplished through NAS chemistry from fluorophenyl-benzothiazoles (F-BTZ-CBz) derivatives to amidated hyperbranched poly(ethyleneimine)(HPEI-IBAm0.61). HPEI is considered to be a synthetic polycation, which promotes tunable solubility through characteristics of …

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Mass Spectrometric Analysis Of Lignin Model Compounds: Fundamental Investigations Of Ionization And Supramolecular Interactions For Lignocellulosic Biomass Applications, Kimberly Dean Jan 2022

Mass Spectrometric Analysis Of Lignin Model Compounds: Fundamental Investigations Of Ionization And Supramolecular Interactions For Lignocellulosic Biomass Applications, Kimberly Dean

Theses and Dissertations--Chemistry

Lignocellulosic biomass is pivotal for the development of renewable energy sources and materials essential to mitigate the exploitation of fossil fuels causing climate change and environmental pollution issues. The conversion of biomass into fuel requires the hydrolysis of cellulose and a biproduct of this process is the isolation of millions of tons of lignin as biorefinery waste. Lignin is a complex high molecular weight polymer whose structure remains undefined and critically limits potential industrial applications of lignocellulosic biomass. The advancement of analytical methods for structural elucidation of lignin and its ensemble of phenolic compounds is therefore essential to advance this …

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A Core–Shell Au@Tio2 And Multi-Walled Carbon Nanotube-Based Sensor For The Electroanalytical Determination Of H2o2 In Human Blood Serum And Saliva, Ayman Ali Saeed, Mohammed Nooredeen Abbas, Waheed Fathi El-Hawary, Yousry Moustafa Issa, Baljit Singh Jan 2022

A Core–Shell Au@Tio2 And Multi-Walled Carbon Nanotube-Based Sensor For The Electroanalytical Determination Of H2o2 In Human Blood Serum And Saliva, Ayman Ali Saeed, Mohammed Nooredeen Abbas, Waheed Fathi El-Hawary, Yousry Moustafa Issa, Baljit Singh

Articles

A hydrogen peroxide (H2O2) sensor was developed based on core–shell gold@ titanium dioxide nanoparticles and multi-walled carbon nanotubes modified glassy carbon electrode (Au@TiO2/MWCNTs/GCE). Core–shell Au@TiO2 material was prepared and characterized using a scanning electron microscopy and energy dispersive X-ray analysis (SEM/EDX), transmission electron microscopy (TEM), atomic force microscopy (AFM), Raman spectroscopy, X-ray diffraction (XRD) and Zeta-potential analyzer. The proposed sensor (Au@TiO2/MWCNTs/GCE) was investigated electrochemically using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The analytical performance of the sensor was evaluated towards H2O2 using differential pulse voltammetry (DPV). The proposed sensor exhibited excellent stability and sensitivity with a linear concentration …

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Dna-Templated Synthesis Of Cationic Styryl Dyes, Kriangsak Faikhruea Jan 2022

Dna-Templated Synthesis Of Cationic Styryl Dyes, Kriangsak Faikhruea

Chulalongkorn University Theses and Dissertations (Chula ETD)

Styryl dye is a class of pi-conjugated dye that shows several desirable optical properties, such as high photostability, solvatochromic characteristics, and high fluorescence quantum yields. Due to the responsiveness to the binding with the target biomolecules such as nucleic acids, some of these dyes have been widely employed for biomolecular detection and cellular imaging. These dyes are typically synthesized via an aldol-type reaction between a methyl heterocycle and an aromatic aldehyde in the presence of acid or base as a catalyst. However, the conventional method of synthesizing these dyes is inefficient, time-consuming, and involves the use of excessive chemicals, as …

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A Nanoscale Shape-Discovery Framework Supporting Systematic Investigations Of Shape-Dependent Biological Effects And Immunomodulation, Wei Zhang, Hender Lopez, Luca Boselli, Paolo Bigini, André Perez-Potti, Zengchun Xie, Valentina Castagnola, Qi Cai, Camila P. Silveira, Joao M. De Araujo, Laura Talamini, Nicolò Panini, Giuseppe Ristagno, Martina B. Violatto, Stéphanie Devineau, Marco P. Monopoli, Mario Salmona, Valeria A. Giannone, Sandra Lara, Kenneth A. Dawson, Yan Yan Dec 2021

A Nanoscale Shape-Discovery Framework Supporting Systematic Investigations Of Shape-Dependent Biological Effects And Immunomodulation, Wei Zhang, Hender Lopez, Luca Boselli, Paolo Bigini, André Perez-Potti, Zengchun Xie, Valentina Castagnola, Qi Cai, Camila P. Silveira, Joao M. De Araujo, Laura Talamini, Nicolò Panini, Giuseppe Ristagno, Martina B. Violatto, Stéphanie Devineau, Marco P. Monopoli, Mario Salmona, Valeria A. Giannone, Sandra Lara, Kenneth A. Dawson, Yan Yan

Articles

Since it is now possible to make, in a controlled fashion, an almost unlimited variety of nanostructure shapes, it is of increasing interest to understand the forms of biological control that nanoscale shape allows. However, a priori rational investigation of such a vast universe of shapes appears to present intractable fundamental and practical challenges. This has limited the useful systematic investigation of their biological interactions and the development of innovative nanoscale shape-dependent therapies. Here, we introduce a concept of biologically relevant inductive nanoscale shape discovery and evaluation that is ideally suited to, and will ultimately become, a vehicle for machine …

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Development Of Visible-Light-Mediated Photoredox Catalysis, Sarbjeet Kaur Dec 2021

Development Of Visible-Light-Mediated Photoredox Catalysis, Sarbjeet Kaur

Legacy Theses & Dissertations (2009 - 2024)

ABSTRACT

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Synthesis Of Phytosulfokine Analogs As Probes For Studying Plant Signaling And Molecular Trafficking, Thomas Ntim Dec 2021

Synthesis Of Phytosulfokine Analogs As Probes For Studying Plant Signaling And Molecular Trafficking, Thomas Ntim

Electronic Theses and Dissertations

Plants are exposed to a wide range of biotic and abiotic stresses that hinder their growth and reduce crop productivity. In their adaptive response, plants use signaling molecules that are trafficked throughout the plant. This research focuses on the chemical synthesis and assessment of analogs of the plant signal phytosulfokine (PSK, a sulfated pentapeptide), its delivery to plants and its observation using a fiber-optic fluorescence microscope. PSK regulates growth, cell expansion, heat tolerance, and tissue longevity. Analogs of PSK were synthesized using solid-phase peptide synthesis. Pure PSK and TAMRA-labeled PSK were delivered into the wild-type Arabidopsis thaliana Col-0 and a …

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Synthesis And Complexation Of Monotosylated 4-Aminopyridine With Nickel (Ii) And Iron (Ii) Ions, Kingsley John Orie, Remy Ukachukwu Duru, Raphael I-Oro Ngochindo Sep 2021

Synthesis And Complexation Of Monotosylated 4-Aminopyridine With Nickel (Ii) And Iron (Ii) Ions, Kingsley John Orie, Remy Ukachukwu Duru, Raphael I-Oro Ngochindo

Makara Journal of Science

Tosylated 4-aminopyridine and other sulfonylated compounds of amines comprise a substantial class of pharmaceutical drugs used as antibiotics in the field of medicine. This research aimed at the synthesis of tosylated 4-aminopyridine and the complexation of the tosyated 4-aminopyridine with Ni(II) and Fe(II) ions. The sulfonamide was prepared by the action of tosyl chloride on 4-aminopyridine in an aqueous alkaline medium. The complexes were synthesized by the reaction of Ni(NO3)2.6H2O /Fe(NO3)2.6H2O with sulfonamide derivative. These compounds were characterized through Ultraviolet Visible spectroscopy (UV–Vis), Fourier Transform Infer-Red (FTIR) spectroscopy, …

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Immobilized-Enzyme Reactors Integrated Into Analytical Platforms: Recent Advances And Challenges, Bert Wouters Dr, Sinead Currivan, Noor Abdulhussain, T. Hankemeier, Peter J. Schoenmakers Aug 2021

Immobilized-Enzyme Reactors Integrated Into Analytical Platforms: Recent Advances And Challenges, Bert Wouters Dr, Sinead Currivan, Noor Abdulhussain, T. Hankemeier, Peter J. Schoenmakers

Articles

Immobilized-enzyme reactors (IMERs) are flow-through devices containing enzymes that are physically confined or localized with retention of their catalytic activities. IMERs can be used repeatedly and continuously and have been applied for (bio)polymer degradation, proteomics, biomarker discovery, inhibitor screening, and detection. Online integration of IMERs with analytical instrumentation, such as high-performance liquid chromatography (HPLC) systems, reduces the time needed for multi-step workflows, reduces the need for sample handling, and enables automation. However, online integration can also be challenging, as reaching its full potential requires complex instrumental setups and experienced users. This review aims to provide an assessment of recent advances …

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Heteroacene-Based Amphiphile As A Molecular Scaffold For Bioimaging Probes, Tharindu A. Ranathunge, Mahesh Loku Yaddehige, Jordan H. Varma, Cameron Smith, Jay Nguyen, Iyanuoluwani Owolabi, Wojciech Kolodziejczyk, Nathan I. Hammer, Glake Hill, Alex Flynt, Davita L. Watkins Aug 2021

Heteroacene-Based Amphiphile As A Molecular Scaffold For Bioimaging Probes, Tharindu A. Ranathunge, Mahesh Loku Yaddehige, Jordan H. Varma, Cameron Smith, Jay Nguyen, Iyanuoluwani Owolabi, Wojciech Kolodziejczyk, Nathan I. Hammer, Glake Hill, Alex Flynt, Davita L. Watkins

Faculty Publications

The challenges faced with current fluorescence imaging agents have motivated us to study two nanostructures based on a hydrophobic dye, 6H-pyrrolo[3,2-b:4,5-b’]bis [1,4]benzothiazine (TRPZ). TRPZ is a heteroacene with a rigid, pi-conjugated structure, multiple reactive sites, and unique spectroscopic properties. Here we coupled TRPZ to a tert-butyl carbamate (BOC) protected 2,2-bis(hydroxymethyl)propanoic acid (bisMPA) dendron via azide-alkyne Huisgen cycloaddition. Deprotection of the protected amine groups on the dendron afforded a cationic terminated amphiphile, TRPZ-bisMPA. TRPZ-bisMPA was nanoprecipitated into water to obtain nanoparticles (NPs) with a hydrodynamic radius that was TRPZ-PG was encapsulated in pluronic-F127 (Mw …

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