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Mir-22 Inhibits The Proliferation, Migration, And Invasion Of Human Cd133-Positive Glioblastoma Stem Cells, Sevi̇l Köse, Özlenen Özkan, Ömer Özkan

Turkish Journal of Biology

Background/aim: Glioblastoma multiforme (GBM) is one of the most aggressive and fatal malignancies of the central nervous system. Despite advancements in treatment strategies, effective therapies for GBM remain insufficient, necessitating further improvements. Notably, miR-22 has been found to be significantly downregulated in both glioblastoma tissues and cell lines. In this study, we aim to evaluate miR-22 expression levels in GBM (U87) and CD133-positive (CD133+) GBM stem cells (GSCs) and to investigate its effects on proliferation, colony formation, migration, invasion, and wound-healing in U87 and CD133+ U87 cells in vitro.Materials and methods: We isolated CD133+ U87 cells using magnetic-activated cell sorting …

Exploring Shootin1’S Oncogenic Role Within Fgfr2 Gene Fusions, Volkan Ergi̇n, Mutlu Erdoğan, Ekrem Yaşar, Sika Zheng

Turkish Journal of Biology

Background/aim: Fibroblast Growth Factor Receptor (FGFR) gene fusions are recognized as pivotal oncogenic drivers, contributing to cancer initiation and progression across diverse malignancies. These fusions often represent significant therapeutic targets, particularly in challenging malignancies like cholangiocarcinoma. This study aimed to characterize the novel FGFR2::SHTN1 fusion, identify it as a de novo chimeric protein, and elucidate its precise oncogenic mechanism. Materials and methods: FGFR2::SHTN1 fusions were identified via cancer genomics databases and modeled using AlphaFold and HADDOCK. SHTN1 variants were expressed in Neuro-2a cells for coimmunoprecipitation, purification, and native polyacrylamide gel electrophoresis to assess oligomerization. Structural modeling included membrane embedding with …

Efflux Pump-Associated Antimicrobial Resistance Genes In Staphylococcus Spp. From Dairy And Meat Samples, M.Burcu Külahci, Sumru Çitak

Turkish Journal of Biology

Background/aim: This study aimed to investigate the phenotypic resistance and distribution of efflux pump-associated antimicrobial resistance genes in Staphylococcus spp. isolated from dairy and meat samples. Antimicrobial resistance in foodborne bacteria increases with antibiotic exposure and biocides, particularly through efflux mechanisms. Thus, monitoring potential genetic reservoirs in the food chain is very important. Materials and methods: A total of 132 dairy and meat samples were collected for the study, and Staphylococcus spp. were isolated using Mannitol salt phenol red agar. Antimicrobial susceptibility was evaluated using the Clinical and Laboratory Standards Institute’s microdilution method. Twenty-six resistant isolates were identified by 16S …

Corrigendum To “Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Diff Egulating The Differentiation Of Cd4+Il-10+T Cells Entiation Of Cd4+Il-10+T Cells Via Tar Via Targeting The Cr Geting The Crosstalk Between Nr Osstalk Between Nrf2 And Nf- F2 And Nf-Κb Signaling Pathways” [Turkish Journal Of Biology 49 (4) 2025 380-391], Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang

Turkish Journal of Biology

This corrigendum is to address an issue regarding the manuscript’s previous publication. The authors noticed that the representative flow cytometry image for the AAA group in Figure 3B, as well as the representative Histone H3 and β-actin Western blot bands in Figure 4A, were incorrectly used in the original published version of this paper and indicated that the relevant corrections do not affect the results or the conclusions of the study.To rectify this oversight and ensure the accuracy of the published work, the correct figures are included for your reference.

Link to the original article: https://doi.org/10.55730/1300-0152.2754

A New Dioecious Bush Tomato, Solanum Nectarifolium (Solanaceae), From The Northern Tanami Desert, Northern Territory, Australia, With Reassessment Of S. Ossicruentum And A Change In The Circumscription Of S. Dioicum, Christopher T. Martine, Kym Brennan, Jason T. Cantley, Aiden Webb, Geoffrey Newton

Faculty Journal Articles

A new species of functionally dioecious bush tomato from the “Solanum dioicum + S. echinatum group” of Solanum subgenus Leptostemonum is described. Solanum nectarifolium Martine & Brennan, sp. nov., is a member of the taxonomically challenging Solanum dioicum W.Fitzg. species complex in Australia and differs from other species in the group by the presence of prominent and conspicuous extrafloral nectaries on the abaxial leaf surfaces, a tinge of purple on the new growth and young fruiting calyces, and curved prickles on young stems. Although fairly well represented in herbarium collections, this new taxon has historically been treated as a …

Eucommia Ulmoides Extract Attenuates Oxidative Stress And Promotes Melanogenesis Via Wnt/Β-Catenin Signaling In B16 Cells And Mice, Xiaojin Liu, Yaqian Qiu, Xiaobing Lv, Lei Chang, Tiancheng Ji, Yefan Gu, Shuyue Chen

Turkish Journal of Biology

Background/aim: Oxidative stress is a major contributor to melanocyte dysfunction and hair graying by impairing key signaling pathways. Eucommia ulmoides bark extract (EUE), rich in antioxidant phytochemicals, has shown potential in combating oxidative damage. This study investigated the protective and promelanogenic effects of EUE under hydrogen peroxide (H₂O₂)-induced oxidative stress, with a focus on the Wnt/β-catenin signaling pathway. Materials and methods: An oxidative stress model was established using B16 cells and a C57BL/6 mouse hair follicle model. Results: EUE significantly improved melanocyte survival and reduced intracellular reactive oxygen species (ROS). Mechanistically, EUE activated the Wnt/β-catenin pathway, leading to upregulation of …

Investigation Of Regulated Cell Death (Rcd) Modalities Induced By Copper(Ii), Manganese(Ii) And Silver(I) Complexes Containing Bridging Dicarboxylate And 1,10-Phenanthroline Ligands, Ella O'Sullivan

Doctoral

With the increasing global cancer burden in recent years, there is an urgency to develop novel chemotherapeutic agents. Transition metal complexes represent an attractive class of candidates, owing to their unique electronic and stereochemical properties, as well as their capacity for ligand exchange, redox activity, and catalytic reactivity. These characteristics enable cellular signalling mechanisms that lead to tumour cell cytotoxicity via regulated cell death (RCD) mechanisms. The conventional RCD mechanisms of apoptosis and autophagy are attractive targets for the development of novel complexes because tumour cells evade apoptosis but use autophagy as a survival mechanism when under stress. Therefore, novel …

(R2150) Impact Of Specialist Predator Harvesting On The Stability Of A Three-Species Food Chain Model With A Generalist Predator, S. Ganga, S. Vijaya

Applications and Applied Mathematics: An International Journal (AAM)

In this study, the stability of a three-species food chain model comprising a prey, an intermediate specialist predator, and a top predator exhibiting generalist behavior is examined, with harvesting applied to the intermediate predator. The main objective is to investigate how variations in the harvesting rate influence system stability and the coexistence of all three species. The positivity, boundedness, and equilibrium points of the model are analyzed to ensure biological feasibility. The equilibrium point involving the prey and the top predator satisfies the criteria for both local and global asymptotic stability. The coexistence equilibrium point attains local asymptotic stability based …

New Geographic Distributional Records For Fishes From The Black River At Black Rock, Lawrence County, Arkansas, C. T. Mcallister, H. W. Robison

Journal of the Arkansas Academy of Science

During February and/or March 2020-2025, various fishes were collected with a modified minnow trap and hoop nets from the White River drainage of the Black River at Black Rock, Lawrence County, Arkansas. A total of 35 species within 11 families were collected and the following taxa represent new distributional records: Acipenseridae: Lake Sturgeon (Acipenser fulvescens); Polydontidae: Paddlefish, Polyodon spathula; Catostomidae: Quillback (Carpiodes cyprinus), Highfin Carpsucker (Carpiodes velifer), Blue Sucker (Cycleptus elongatus), Northern Hogsucker (Hypentelium nigricans); Leuciscidae: Creek Chub (Semotilus atromaculatus). The most significant discoveries were the collection …

Enhanced Oviposition Preferences And Larval Performance Of Hermetia Illucens L. In Fruit Wastes Fermented By Effective Microorganisms, Resti Rahayu, Robby Jannatan, Muhamad Ronaldo, Risa Ukhti Muslima

Makara Journal of Science

Organic waste comprises a considerable portion of global waste, requiring effective management. The ability of the black soldier fly (Hermetia illucens, BSF) to convert organic waste into biomass has been well reported. However, bioconversion can be hindered by fiber-rich substrates, a challenge that can be addressed through substrate pretreatment. This study examined the oviposition preferences and larval performance of BSFs in several fruit wastes fermented by effective microorganisms (EMs), which were compared to non-fermented substrates. The oviposition attractants utilized pineapple peel and overripe soursop, with larval performance evaluated among different treatments, i.e., non-fermented pineapple peel (NFP), fermented pineapple …

Some New Ecological Aspects Of The Lesser Sunda Endemic Drepanosticta Berlandi (Odonata: Zygoptera: Platystictidae), Muhammad Zulhariadi, Muhammad Setyo Nugroho, Nurul Hidayani

Makara Journal of Science

Drepanosticta berlandi is an endemic damselfly in the Lesser Sunda region of Indonesia, which was first recorded on Lombok Island in the 19th century. Albeit recognized for a century, information on its ecology is still lacking; hence, this study aims to reveal its distribution and habitat preferences. The research was conducted at the Tibu Ijo Waterfall tourist area in West Lombok Regency using line transects combined with field survey in the Kekait River and on footpaths on both sides of the river. Vegetation density, topography, and land survey temperature (LST) were measured at the sampling area using ArcGIS 10.4.1 software. …

Neuroprotective Effects Of Aqueous Extract Of Bryophyllum Pinnatum Against Lead Acetate Induced Damage In The Cerebellum Of Rats, O. A. Bamgbose, O. D. Akanji, E. O. Olayemi, D. O. Taiwo-Ola, K. O. Odubela, B. A. Akanbi, P. B. Fakunle

AUIQ Complementary Biological System

Lead is a persistent environmental toxin with pronounced neurotoxic effects, particularly on the central nervous system. This study evaluated the impact of lead acetate on the cerebellar cortex of adult Wistar rats and assessed the neuroprotective effects of aqueous Bryophyllum pinnatum extract. Twenty adult Wistar rats (180–220 g) were randomly assigned to four groups (n = 5): Control (distilled water), T1 (0.075 mg/kg lead acetate), T2 (100 mg/kg B. pinnatum), and T3 (lead + B. pinnatum). Treatments were administered orally for 21 days. Cerebellar tissues were processed for biochemical and histological analysis, and data were analyzed using GraphPad Prism v10.1.Lead …

Cytological Changes In Buccal Mucosa Among Yemeni Qat Chewers In Hadhramout Governorate: A Comparative Cross-Sectional Study, Saleh H. Ben Sumaidea, Hassan Pyar, Abdullah O. Bin Nashwan, Abdullah M. Bin Fadhl, Abeer A. Marabi, Ahmed A. Al-Shetri, Gumaan S. Rassam, Halima K. Joher, Mashhoor A. Bin Shehab, Mohammed S. Bin Mosalem

AUIQ Complementary Biological System

Qat (Catha edulis) chewing is a common practice in Yemen, associated with oral mucosal irritation. The objective of the current study is to compare cytological findings of buccal mucosa between Qat chewers and non-chewers. A comparative cross-sectional study was designed. Buccal smears were collected from chewers (n = 100) and non-chewers (n = 100), fixed, and stained with Harris hematoxylin staining. Smears were assessed for inflammatory changes and cytological atypia by two blinded examiners; disagreements were resolved by consensus. The inflammatory cells were observed in 21% of chewers compared to 8% of controls (P = 0.009), while …

Advancing Skin Cancer Diagnostics: A Comparative Analysis Of Convolutional Neural Network Models For Skin Lesion Classification, Jesse Osuji, Tania Malik

SURE Journal: Science Undergraduate Research Experience Journal

For many years, skin cancer diagnosis and cancer research have been major focal points in the healthcare sector. Accurate classification of different types of skin lesions, including cancerous ones, is essential for proper diagnostics. Many machine learning (ML) techniques and models are used to solve various problems across various domains. One of such techniques is called neural networks. Convolutional Neural Networks (CNNs) are primarily used in computer vision-based problems, allowing computer systems to process and understand images. CNNs offer a potential tool for diagnosing skin lesions from images, occasionally demonstrating superior accuracy compared to trained dermatologists. This project explores various …

Developing A Tool To Measure Resistance To Sustainable Food Choices: A Pilot Study In Ireland And The Netherlands, Kate Flanagan, Bart Sinteur, Patricia J. Bulsing, John M. Kearney

SURE Journal: Science Undergraduate Research Experience Journal

Consumer uptake of a sustainable diet is critical for addressing environmental challenges and promoting public health. Understanding why consumers resist adopting sustainable eating habits can inform targeted interventions and policies. This pilot study examines whether a newly developed scale can effectively detect consumer resistance to a sustainable diet. Currently, there is a lack of research on a tool to measure consumer resistance to a sustainable diet, highlighting the need for a reliable instrument.

The aim of this study is to develop a scale, based on existing literature, to measure consumer resistance to a sustainable diet. Additionally, the study aims to …

Editorial-Sure Journal Volume 7, Issue 2, Geraldine M. Dowling, Eva Campion, Sinead Loughran, Ariane Perez-Gavilan, Josephine Treacy

SURE Journal: Science Undergraduate Research Experience Journal

No abstract provided.

Chaos Engineering In Multi-Gigahertz Solid-State Lasers: A Novel Approach To Optoelectronic Control, Mikhail V. Gorbunkov, Yulia Ya. Maslova, Yulia A. Sinichkina

Karbala International Journal of Modern Science

This paper presents a comprehensive study of a multi-gigahertz chaotic generator of light pulses based on solid-state laser sources, including fiber lasers, governed by carefully designed positive and negative feedback loops. It harnesses the inherent nonlinear dynamics within a solid-state laser controlled by a combination of two inertial feedback loops, enabling the realization of complex chaotic behavior, including the logistic map scenario, under moderate amplification conditions. The laser system dynamics are rigorously investigated through theoretical modeling, employing a nonlinear map approach, and high-resolution picosecond simulations. The results of our numerical simulations highlight the efficacy of fast electro-optical feedback system with …

Unveiling The Anticancer Potential Of Syzygium Cumini: In Silico Insights Into Its Mechanistic Action Against Non-Small Cell Lung Cancer, Nur Sofiatul Aini, Win Darmanto

Karbala International Journal of Modern Science

Non-small cell lung carcinoma (NSCLC) is the most periodic type of lung cancer and the second most diagnosed cancer globally. Syzygium cumini is plant that extensively used in cuisine and traditional medicine. However, its potential for NSCLC treatment has not yet been elucidated. This study determined the potential of S. cumini as anti-NSCLC using in silico approaches. The in silico study was applied to perform active compound analysis, selection of target candidates, network pharmacology, functional annotation, molecular docking, and molecular dynamics simulation, respectively. Based on open source databases, S. cumini contained 115 compounds and 14 of them predicted to have …

Substantial Contribution Of Woody Components To Rainfall Interception In Chinese Forests: Insights From A Refined Analytical Model, Zhi-Ang Chen, Zhi-Yun Jiang, Wei He, John T. Van Stan Ii, Et. Al.

Biological, Geological, and Environmental Faculty Publications

Assessing rainfall interception (I-R) is a critical yet uncertain aspect in hydrological cycle, particularly the quantification of relative contributions from leaves and woody components (e.g., branches, stems, and trunks) to I-R. Nevertheless, the role of woody components in I-R estimation remains largely unexplored and thereby has been constantly overlooked. This study addressed this challenge and refined the widely-used Gash model to distinguish woody interception (I-W) from leaf interception (I-L). We incorporated the spatial variability of vegetation traits alongside satellite data in 2019 into the refined model, and spanned China's major forest types. The refined model showed a strong agreement with …

Immunoglobulin Germline Reference Sequences Enable Investigations And Reveal Insights Into Bat-Specific Immunity, Ashley B. Reers, Shijun Zhan, Taylor Pursell, Clara Reasoner, Natasha Hodges, Tanya M. Lama, Tony Schountz, Hannah K. Frank

Biological Sciences: Faculty Publications

We generated a highly contiguous, annotated genome of the Jamaican fruit bat, Artibeus jamaicensis, including annotated germline immunoglobulin heavy-chain (IGH) and light-chain lambda (IGL) loci to understand bat B cell receptor repertoires. The bat germline shares many structures and features described in human immunoglobulin loci. However, some features are unique to A. jamaicensis, including an expansion of cysteine-rich IGHV genes. To investigate the relationship between the germline IGH locus and expressed B cell receptors (BCRs), we sequenced the BCRs of wild-caught and captive A. jamaicensis, finding an enrichment of IGHV3 and IGHV4 genes. Compared to humans, A. jamaicensis had shorter …

Public Perception About Covid-19 Vaccine Hesitancy In South Central Nebraska, Rita Nnenne Oparaocha

Biology Theses, Dissertations, and Student Creative Activity

The COVID-19 pandemic created a shift in the trajectory of vaccination hesitancy, altering risk perceptions and vaccination attitudes among individuals in rural communities. The 5C framework highlights confidence, complacency, constraints, calculation, and collective responsibility as influencing factors of vaccine hesitancy. This study assessed the proportion of adults who refused COVID-19 vaccination and identified the individual reasons for hesitancy among those who refused the COVID -19 vaccines in South Central Nebraska. Data was analyzed from a local cross-sectional public health survey administered to 2,034 adult participants over a six-month period (March-August 2024), including 91 respondents who were from outside the seven-county …

Comparisons Of Fish Communities Between A Restored And A Control Pool Of The Mississippi River: A Before-And-After Examination, Hannah Lenning

Biology Theses, Dissertations, and Student Creative Activity

The quality and availability of valuable backwater habitats have declined in many rivers, including the Upper Mississippi River (UMR), due to river modifications and land use change. Habitat Rehabilitation and Enhancement Projects (HREPs) are implemented by the Upper Mississippi River Restoration Program and often aim to mitigate accelerated sedimentation and degradation of backwater habitats. One HREP in Pool 12 of the UMR targeted overwintering fish habitat by restoring four backwaters within 14 RKM. Fish community data was collected before (2006-2012) and after (2020-2024) restoration in Pool 12 and a downstream, control reach, Pool 13. The first objective of my study …

A Genetic Interaction Between Ded1 And Hat1 In Saccharomyces Cerevisiae Reveals A Role For Hat1p In Cytoplasmic Rna Granule Accumulation, Audrey J. Panko, Aidan Winters, Nicholas R. Rothbard, Angela K. Hilliker

Biology Faculty Publications

Ded1p is an essential translation initiation factor that interacts with and is modulated by eIF4F. Ded1p also promotes the assembly and disassembly of stress granules, which contain nontranslating mRNAs and translation initiation factors. As Ded1p affects both mRNA storage and translation initiation, regulation of Ded1p's function may affect whether mRNAs are translated or localized to cytoplasmic RNA granules. To identify regulators of Ded1p in Saccharomyces cerevisiae, we screened for overexpression suppressors of the severe growth defect conferred by high levels of Ded1p. We found that overexpression of HAT1, a lysine acetyltransferase, can suppress the growth defect conferred by …

Elucidating The Role Of Hd5 In Colonic Wound Healing, Lavin Radiro

Student Scholar Symposium

Crohn’s colitis (CC), a subtype of inflammatory bowel disease (IBD), causes chronic inflammation in the large intestine, leading to malnutrition, diarrhea, and severe pain. Early and accurate diagnosis of CC is crucial, as misdiagnosis with ulcerative colitis (UC) can result in inappropriate treatment and worsened outcomes. The innate immune peptide human alpha-defensin 5 (HD5) is upregulated in CC but not in UC, suggesting a potential role in disease pathogenesis. Preliminary data from our lab indicates that HD5 impairs wound healing in colonic epithelial cells, but the molecular mechanisms remain unclear.

Hypothesis We hypothesize that HD5 increases RNA and protein levels …

Investigating The Potential Ability Of The Tlr4-Nf-Κb Axis To Reduce Aryl Hydrocarbon Receptor Expression In Intestinal Epithelial Cells, Gabriella Longoria

Student Scholar Symposium

Necrotizing enterocolitis (NEC) primarily affects premature infants and is characterized by intestinal necrosis, inflammation, and high mortality. Toll-like receptor 4 (TLR4)-induced apoptosis of intestinal epithelial cells is thought to drive NEC development. Upon binding bacterial lipopolysaccharide (LPS), TLR4 signaling activates NF-κB, leading to inflammatory gene expression. Indole-3-carbinol (I3C), a pro-ligand of the aryl hydrocarbon receptor (AhR), prevents NEC in a mouse model by downregulating TLR4, despite reduced AhR expression in NEC-affected intestines. However, the molecular mechanism behind the reduced expression of AhR in NEC is unclear. To model NEC in vitro, we treated IEC-6 enterocytes with LPS, which reduces AhR …

Investigating The Role And Mechanism Of Faecalibacterium Prausnitzii In Fatty Acid Metabolism And Obesity Using The Drosophila Melanogaster Model, Jackson Head, Whitley Whitehead

Student Scholar Symposium

The obesity epidemic has increased to enormous numbers with 890 million adults being classified as obese (BMI≥30). Obesity is of extreme concern as it is a comorbidity to coronary heart disease, type-2 diabetes, and cancer. Although obesity has classically been attributed to genetics and lifestyle, there is a growing interest in other factors, including the gut microbiome. Germ-free mice are resistant to weight gain even when placed on a high-calorie diet, establishing that metabolites produced by gut microbiota can dramatically inhibit metabolism. Here, we use Faecalibacterium prausnitzii, which has been previously shown to be reduced in obese individuals; however, …

Indole Improves Outcomes Of Cronobacter Sakazakii Infection In C. Elegans Through Ahr-1, Cayden Durrough

Student Scholar Symposium

Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease primarily affecting premature infants. Toll-like receptor 4 (TLR4)-mediated epithelial damage allows microbial translocation, triggering inflammation that can lead to sepsis and death. The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor, and AhR ligands have been shown to prevent NEC in a mouse model. Indole, an AhR ligand, has been reported to extend the healthspan of C. elegans, and we have shown previously it mitigates poor outcomes in wild-type worms infected with Cronobacter sakazakii (Cs), a bacterial pathogen linked to NEC. We hypothesized that C. elegans must express ahr-1, the ortholog …

Indole Treatment Of C. Elegans Rescues Cronobacter Sakazakii Induced Illness, Joshua Asselin

Student Scholar Symposium

Necrotizing enterocolitis (NEC) is a severe and often life-threatening inflammatory condition of the intestine primarily affecting premature infants. The opportunistic pathogen Cronobacter sakazakii (Cs) has been implicated in NEC. While activation of the ligand-dependent transcription factor aryl hydrocarbon receptor (AhR) has been shown to prevent NEC in a mouse model, its potential as a therapeutic target for established NEC remains unclear. We hypothesize here that the AhR ligand indole can reverse Cs-mediated illness in the model organism Caenorhabditis elegans. Our results demonstrate that exposure to live Cs, but not heat-killed Cs, significantly impaired worm motility, demonstrating Cs is an effective …

Function Of Drosophila Melanogaster Domesticated Transposable Element Protein Dplg3 In Oogenesis And Total Fitness, Aron Herrera

2025 Fall Honors Capstones Projects - Archive

Transposable elements (TEs) are selfish DNA segments capable of self-replication and reinsertion within genomes, often harming hosts. Rarely, host genomes can adopt TEs and use their proteins for new cellular functions through molecular domestication. One example primarily expressed in Drosophila melanogaster ovaries is DPLG3. Previous Betrán Lab students generated DPLG3 loss-of-function flies and found that their ovaries misregulated one primordial and 14 testis-specific genes, leading to variable ovarian malformations. However, it is unclear when during oogenesis these misexpressions occur. We will extract RNA from non-rudimentary ovaries at early and late stages to generate cDNA for qRT-PCR analysis of three …

Impact Of Gas Supersaturation On The Development Of Gas Bubble Trauma In Paedomorphic Eurycea Salamanders, Trent Furr

Biology Theses

There are eleven species of paedomorphic lungless salamanders endemic to Texas. Of these, eight are considered threatened by the International Union for Conservation of Nature (IUCN). One of these, the Barton Springs Salamander (Eurycea sosorum), is found only in springs and aquifers in and around Austin, Texas. A large population of this species is found in Barton Springs, located in Austin, TX–where dissolved gas saturation has been measured as high as 134%. Morbidity and mortality events in Barton Springs Salamanders have been documented coinciding with periods of dissolved gas supersaturation. This study seeks to understand what environmental conditions lead to …