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Using Scratch Wound Assay To Study The Effect Of Soil Arsenic On Human Dermal Fibroblasts Cell Migration Due To Contact Exposure, Manas Warke, Laura De Marchi, Srinivas Kannan, Madeline English, Rohan Sarkar, Rupali Datta, Smitha Rao

Michigan Tech Research Data

The scratch wound assay was performed on Normal Human Primary Dermal Fibroblasts (HDFa) cells to observe the effect on cell migration due to contact exposure to arsenic-contaminated Immokalee soil. The cell migration was observed through a microscope for 72 h. HDFa cells were seeded in 48-well plate. On day 3, treatment media was added (n=8). The cells were treated with four concentrations of soil As (45, 225, 450, and 900 mg/kg) and two controls - Negative control (NC; Pure media) and control (C; 0 mg/kg soil As) for 72 h. A scratch was made using a pipette tip. The wound …

Olfactory Detection Of Viruses Shapes Brain Immunity And Behavior, Aurora Kraus

Biology ETDs

Olfactory sensory neurons (OSNs) directly contact the environment and are

exposed to pathogens, such as viruses. When OSNs detect a virus, they coordinate antiviral immune responses locally to stop virus progression into the brain, termed the central nervous system (CNS). For example, in COVID-19 patients the SARS-CoV-2 virus replicates in the olfactory epithelium resulting in loss of olfaction, yet viral presence in the CNS is rare. However, neuronal detection of a virus by OSNs may send electrical signals to the CNS via the olfactory bulb (OB) and shape our CNS. Because the OB is the nexus between the pathogen exposed …

Post-Embryonic Phase Transitions Mediated By Polycomb Repressive Complexes In Plants, Valerie Hinsch, Samuel Adkins, Darren Manuela, Mingli Xu

Faculty Publications

Correct timing of developmental phase transitions is critical for the survival and fitness of plants. Developmental phase transitions in plants are partially promoted by controlling relevant genes into active or repressive status. Polycomb Repressive Complex1 (PRC1) and PRC2, originally identified in Drosophila, are essential in initiating and/or maintaining genes in repressive status to mediate developmental phase transitions. Our review summarizes mechanisms in which the embryo-to-seedling transition, the juvenile-to-adult transition, and vegetative-to-reproductive transition in plants are mediated by PRC1 and PRC2, and suggests that PRC1 could act either before or after PRC2, or that they could function independently of each other. …

Population Genetics Of Cownose Rays, Rhinoptera Spp. In The Western Atlantic, Helen Weber

Master's Theses

Using molecular tools (e.g., the NADH subunit II mitochondrial gene), this study was the first to document the Brazilian cownose ray, Rhinoptera brasiliensis, within the northwestern Atlantic (NWA), and was the first study to examine population structure in R. brasiliensis within the NWA or northern Gulf of Mexico (GMX), revealing novel insights into the population biology of the animal and extending its range by nearly 1,500 km. This study also examined the sympatrically occurring American cownose ray, R. bonasus, and found population structure between the NWA and the GMX and the NWA and the southwestern Atlantic (SWA). High …

Assessing The Effects Of Elevation On The Peaks Of Otter Salamander (Plethodon Hubrichti) Using Body Condition Index, Norman Reichenbach, Timothy R. Brophy, Hannah Suber, Elisabeth Russell, Hannah Kinsley, Liam Cusack, Cetia Dawson, Savannah Dunn, Olivia De Araujo

Faculty Publications and Presentations

The Peaks of Otter Salamander (Plethodon hubrichti; POS) is a montane species found at elevations above 442 m within a 117 km2 area of the Blue Ridge Mountains in central Virginia. In allopatric areas (areas without the Eastern Red-backed Salamander, P. cinereus, a known competitor), POS body condition was hypothesized to decline both above and below some optimal elevation. Decreased condition at lower elevations would most likely be due to increased temperatures and lower relative humidities, which may adversely affect the ability of salamanders to forage effectively on vegetation due to desiccation risk. Decreased condition at elevations above …

Abundance, Site-Fidelity, And Association Patterns Of Coastal Bottlenose Dolphins (Tursiops Truncatus) Off Southeast Florida, Graysen D. Boehning

All HCAS Student Capstones, Theses, and Dissertations

The coastal bottlenose dolphin is well studied throughout its natural range, however, most of the study areas comprised wide, well-protected habitats such as bays and estuaries, and not narrow coastal sandbanks. This study identifies a residential group of coastal bottlenose dolphins utilizing the narrow sandbanks within the Northwestern Atlantic waters off the coast of Palm Beach County, Florida, USA. From 2014-2020, 313 boat surveys were conducted, and 585 individual dolphins were identified using photo-ID. Twenty-four animals were determined to be full-time and 66 animals were determined to be part-time residents. Full-time and part-time residents associated in three social tribes, with …

Consequences Of Gulf Of Mexico Estuarine Tropical Cyclone Disturbance Regimes For Fish Assemblage Dynamics Conditionally Depend On Life-History Traits And Niche Characteristics, Stephen Edward Potts

LSU Doctoral Dissertations

Tropical cyclones (TC) are intense, localized disturbances that can potentially cause extensive damage to ecosystems. This dissertation is motivated by a need for interdisciplinary study of TC-ecosystem dynamics with applications to ecology, geography, and analytical methodology. I explore concept linkages between TC dynamics and ecosystems to characterize TC-ecosystem relationships as ecological disturbance regimes. This dissertation tests hypotheses and relationships of TC effects on estuarine fish assemblages in the north-western Gulf of Mexico. I explore concepts in TC-ecosystem relationships to link biotic dynamics with physical disturbance.

In Chapter 2, I compared spatial performance of generalized linear models (GLMs) and generalized additive …

Ci Asteroid Regolith As An In Situ Plant Growth Medium For Space Crop Production, Steven J. Russell, Sherry Fieber-Beyer, Kathryn A. Yurkonis

Biology Faculty Publications

Human expansion into the solar system is currently at the forefront of space research. For our astronauts to survive, they will need to be fed a healthy and nutritious diet on a consistent basis. Right now, our current method of feeding astronauts consists of resupplied prepackaged food from Earth, which is unsustainable for long-term missions. Using planetary resources via in situ resource utilization to grow crops is the next step toward sustainability in space. Asteroids are an abundant space resource and should not be overlooked when considering crewed missions. In particular, the primordial CI carbonaceous asteroids are of interest because …

Relating Plant Community Structure To Carbon Dynamics In Semiarid Grasslands, Theodore D. Roper

Biology ETDs

Understanding how fine-scale changes in soil characteristics and plant community composition affect ecosystem functioning is key to predicting how biome shifts will affect regional and global carbon cycling. This is crucial in the dryland biomes of the US Southwest, projected to be one of the regions most affected by climate change. We examined fine-scale drivers of ecosystem function within two biomes – a Chihuahuan Desert grassland and Plains/Chihuahuan Desert ecotone – via long-term vegetation data, micrometeorological data, eddy covariance carbon flux measurements, and soil water and texture, finding that the ecotone site had over 30% higher soil water content, over …

Insights Into The Speciation Process From Genomic And Phenotypic Analysis Of An Avian Hybrid Zone In Amazonia, Glaucia Christina Del-Rio

LSU Doctoral Dissertations

Understanding the outcome of secondary contact is essential to shed light on the mechanisms governing species formation and maintenance. In Amazonia, closely related bird taxa with limited dispersal abilities are often separated by rivers, which presumably act as dispersal barriers. However, at the headwaters, rivers cease to be dispersal barriers, and this generates opportunities for secondary contact. In my dissertation, I studied genomic mechanisms associated with phenotypic differences, mitochondrial DNA structure, and putative reproductive barriers between two hybridizing Amazonian bird species in the genus Rhegmatorhina, a group of antbirds that find their arthropod prey exclusively by following army-ant swarms. …

Biological Flora Of Coastal Wetlands: Sporobolus Cynosuroides (L.) P.M. Peterson & Saarela, Richard Stalter, Robert I. Lonard

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Sporobolus cynosuroides (L.) P.M. Peterson & Saarela¼Spartina cynosuroides (L.) Roth is a temperate zone rhizomatous grass that often is a dominant species in coastal brackish marshes on the Gulf coast and Atlantic coasts of the United States where salinity ranges from 0 to 10 psu. Sporobolus cynosuroides (L.) P.M. Peterson & Saarela ¼ Spartina cynosuroides (L.) Roth is usually absent where salinity values are .12 psu. Sporobolus cynosuroides occurs in coastal habitats characterized by infrequent tidal flooding and moderate nutrient levels. Also known as big cordgrass, it may account for net productivity in high marshes that rivals productivity of Sporobolus …

Khsrp Loss Increases Neuronal Growth And Synaptic Transmission And Alters Memory Consolidation Through Rna Stabilization, Sarah L. Olguin, Priyanka Patel, Courtney N. Buchanan, Michela Dell'orco, Amy S. Gardiner, Robert Cole, Lauren S. Vaughn, Anitha Sundararajan, Joann Mudge, Andrea M. Allen, Pavel Ortinski, Jonathan L. Brigman, Jeffery L. Twiss, Nora L. Perrone-Bizzozero

Faculty Publications

KH-type splicing regulatory protein (KHSRP) regulates levels of several neuronal mRNAs linked to nervous system development and function.

The KH-type splicing regulatory protein (KHSRP) is an RNA-binding protein linked to decay of mRNAs with AU-rich elements. KHSRP was previously shown to destabilize Gap43 mRNA and decrease neurite growth in cultured embryonic neurons. Here, we have tested functions of KHSRP in vivo. We find upregulation of 1460 mRNAs in neocortex of adult Khsrp(-/-) mice, of which 527 bind to KHSRP with high specificity. These KHSRP targets are involved in pathways for neuronal morphology, axon guidance, neurotransmission and long-term memory. Khsrp(-/-) mice …

Extracellular Domain Of Pept1 Interacts With Tm1 To Facilitate Substrate Transport, Jiemin Shen, Miaohui Hu, Xiao Fan, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou

Faculty, Staff and Students Publications

Mammalian peptide transporters, PepT1 and PepT2, mediate uptake of small peptides and are essential for their absorption. PepT also mediates absorption of many drugs and prodrugs to enhance their bioavailability. PepT has twelve transmembrane (TM) helices that fold into an N-terminal domain (NTD, TM1–6) and a C-terminal domain (CTD, TM7–12), and has a large extracellular domain (ECD) between TM9–10. It is well-recognized that peptide transport requires movements of the NTD and CTD, but the role of the ECD in PepT1 remains unclear. Here we report the structure of horse PepT1 encircled in lipid nanodiscs and captured in the inward-open apo …

Leafing Through Literature: Late Embryogenesis Abundant Proteins Coming Of Age – Achievements And Perspectives, Itzell E. Hernandez-Sanchez, Israel Maruri Lopez, Coral Martinez-Martinez, Brett Janis, Juan Francisco Jimenez Bremont, Alejandra A. Covarrubias, Michael A. Menze, Steffen P. Graether, Anja Thalhammer

Faculty and Staff Scholarship

To deal with increasingly severe periods of dehydration related to global climate change, it becomes increasingly important to understand the complex strategies many organisms have developed to cope with dehydration and desiccation. While it is undisputed that late embryogenesis abundant (LEA) proteins play a key role in the tolerance of plants and many anhydrobiotic organisms to water limitation, the molecular mechanisms are not well understood. In this review we recap the current knowledge of the physiological roles of LEA proteins and discuss their potential molecular functions. As these are ultimately linked to conformational changes in the presence of binding partners, …

Cd169 (Siglec-1) As A Robust Human Cell Biomarker Of Toll-Like Receptor 9 Agonist Immunotherapy, Stine Sofie Frank Lende, Marie H. Pahus, Ida Monrad, Rikka Olesen, Anna Mahr, Line K. Vibholm, Lars Østergaard, Ole Schmeltz Søgaard, Anna Halling Folkmar Andersen, Paul Denton, Martin Tolstrup

Biology Faculty Publications

Immunotherapy is a promising therapeutic area in cancer and chronic viral infections. An important component of immunotherapy in these contexts is the activation of innate immunity. Here we investigate the potential for CD169 (Siglec 1) expression on monocytes to serve as a robust biomarker for activation of innate immunity and, particular, as a proxy for IFN-α production. Specifically, we investigated the effects of Toll-like receptor 9 agonism with MGN1703 (lefitolimod) across experimental conditions ex vivo, in humanized mice, and in clinical trial participants. Ex vivo we observed that the percentage of classical monocytes expressing CD169 increased dramatically from …

Coordinating Tiny Limbs And Long Bodies: Geometric Mechanics Of Lizard Terrestrial Swimming, Baxi Chong, Tianyu Wang, Eva Erickson, Philip J. Bergmann, Daniel I. Goldman

Biology

Although typically possessing four limbs and short bodies, lizards have evolved diverse morphologies, including elongate trunks with tiny limbs. Such forms are hypothesized to aid locomotion in cluttered/fossorial environments but propulsion mechanisms (e.g., the use of body and/or limbs to interact with substrates) and potential body/limb coordination remain unstudied. Here, we use biological experiments, a geometric theory of locomotion, and robophysical models to investigate body–limb coordination in diverse lizards. Locomotor field studies in short-limbed, elongate lizards (Brachymeles and Lerista) and laboratory studies of fully limbed lizards (Uma scoparia and Sceloporus olivaceus) and a snake (Chionactis occipitalis) reveal that body-wave dynamics …

Leaf Development In Medicago Truncatula, Liren Du, Samuel Winright Adkins, Mingli Xu

Faculty Publications

Forage yield is largely dependent on leaf development, during which the number of leaves, leaflets, leaf size, and shape are determined. In this mini-review, we briefly summarize recent studies of leaf development in Medicago truncatula, a model plant for legumes, with a focus on factors that could affect biomass of leaves. These include: floral development and related genes, lateral organ boundary genes, auxin biosynthesis, transportation and signaling genes, and WOX related genes.

Structure-Activity Relationship And Antitumor Activity Of 1,4-Pyrazine-Containing Inhibitors Of Histone Acetyltransferases P300/Cbp, Shenyou Nie, Fangrui Wu, Jingyu Wu, Xin Li, Chao Zhou, Yuan Yao, Yongcheng Song

Faculty, Staff and Students Publications

Acetylation of histone lysine residues by histone acetyltransferase (HAT) p300 and its paralog CBP play important roles in gene regulation in health and diseases. The HAT domain of p300/CBP has been found to be a potential drug target for cancer. Compound screening followed by structure-activity relationship studies yielded a novel series of 1,4-pyrazine-containing inhibitors of p300/CBP HAT with their IC50s as low as 1.4 μM. Enzyme kinetics and other studies support the most potent compound 29 is a competitive inhibitor of p300 HAT against the substrate histone. It exhibited a high selectivity for p300 and CBP, with negligible activity on …

Interspecific Differences In The Flow Regimes And Drag Of North Pacific Skate Egg Cases, Kayla C. Hall, Jaida N. Elcock, Gerald R. Hoff, Duane E. Stevenson, Adam P. Summers, Cassandra M. Donatelli

Engineering Faculty Articles and Research

Skates are a diverse group of dorso-ventrally compressed cartilaginous fishes found primarily in high-latitude seas. These slow-growing oviparous fishes deposit their fertilized eggs into cases, which then rest on the seafloor. Developing skates remain in their cases for 1-4 years after they are deposited, meaning the abiotic characteristics of the deposition sites, such as current and substrate type, must interact with the capsule in a way to promote long residency. Egg cases are morphologically variable and can be identified to species. Both the gross morphology and the microstructures of the egg case interact with substrate to determine how well a …

Ants And Spices: The Potential Of Spices To Repel Pest Ants (Formicidae), Jenny Vu, Hritam Mitra, Joe Steven Hardy, Nirupama Chauhan

2022 REYES Proceedings

We examined the spices nutmeg, cinnamon, Ngo Hiang Spice Mix (anise, galangale, cinnamon and star anise) and turmeric for repellent activity against foraging ants. Control baits consisting of a cracker substrate topped with granulated sugar were presented next to treatment baits (cracker, granulated sugar and spice spread over the sugar) to ants in natural habitats. After an hour, the numbers of ants recruiting to the control and treatment baits were recorded. Jenny Vu first tested nutmeg and then cinnamon; Hritam Mitra tested turmeric; Joe Steven Hardy tested Ngo Hiang Spice Mix and Nirupama Chauhan tested turmeric. Data were analyzed with …

Water Availability Dictates How Plant Traits Predict Demographic Rates, Alice E. Stears, Peter B. Adler, Dana M. Blumenthal, Julie A. Kray, Kevin E. Mueller

Biological, Geological, and Environmental Faculty Publications

A major goal in ecology is to make generalizable predictions of organism responses to environmental variation based on their traits. However, straightforward relationships between traits and fitness are rare and likely to vary with environmental context. Characterizing how traits mediate demographic responses to the environment may enhance the predictions of organism responses to global change. We synthesized 15 years of demographic data and species-level traits in a shortgrass steppe to determine whether the effects of leaf and root traits on growth and survival depended on seasonal water availability. We predicted that (1) species with drought-tolerant traits, such as lower leaf …

Ants And Spices: Multiple Bioassays Investigating Turmeric (Curcuma Longa) And Chili Powder As Repellents For Ants (Formicidae), Luca Ben Yishay-Sapalio

2022 REYES Proceedings

Spices offer an environmentally friendly method of controlling ants (Formicidae) in homes. In this study, I used three bioassays to examine the effect of Turmeric (Curcuma longa) and one bioassay to examine the effect of Chili Powder as repellents for ants. Tests were conducted near active ants outdoors. All bioassays examined paired control and treatment baits along a transect, and control baits consisted of a small sheet of corrugated cardboard (a sterile yet easy to manipulate substrate) with granulated sugar on top. In the first bioassay, treatment baits were similar to controls but with Turmeric covering the sugar. …

Evaluation Of Silver Nanoparticles Attached To Methylene Blue As An Antimicrobial Agent And Its Cytotoxicity, Somon Hakimov

Masters Theses & Specialist Projects

Antibiotics resistance is considered as one of the greatest public health challenges of our time. In this work, we have synthesized silver nanoparticles (Ag NPs) using pulsed liquid ablation in different medium of growth and later combined with methylene blue (MB) to evaluate its potential as an effective photodynamic therapy agent. Ag NPs were synthesized by pulsed laser ablation technique in Polyvinylpyrrolidone (PVP), citrate, and Polyvinyl alcohol (PVA). The Ag NPs were characterized using transmission electron microscopy (TEM), UV-Visible (UV-Vis), and photoluminescence (PL) spectra. Next, Ag NPs were coupled with MB and used to deactivate the Gram-negative bacteria, Escherichia coli …

Analysis Of Hawk Mountain Sanctuary Observation Data From 1976 Through 2021, Dale E. Parson

Computer Science and Information Technology Faculty

The primary objective is to correlate climate change data to changes in raptor observations at the Hawk Mountain Sanctuary in northern Berks County, Pennsylvania. Additional objectives include uncovering trends in climate observations at Hawk Mountain's North Lookout and the Allentown Airport throughout the observation period, and to examine trends in raptor observation properties independent of climate changes.

2022 Spring/Summer Newsletter, Plaster Creek Stewards

Newsletters

Highlights:

Staff Transitions

Spring Event

Summer Students

Green Team

Reporting Suspicious Pollutants

Spring & Summer School Partners

Watershed Support Grant #2 Awarded

Macroinvertebrate Study Volunteers Needed

Nature, Data, And Power: How Hegemonies Shaped This Special Section, A. Kamath, B. Velocci, A. Wesner, N. Chen, Vincent A. Formica, B. Subramaniam, M. Rebolleda-Gómez

Biology Faculty Works

Systems of oppression—racism, colonialism, misogyny, cissexism, ableism, heteronormativity, and more—have long shaped the content and practice of science. But opportunities to reckon with these influences are rarely found within academic science, even though such critiques are well developed in the social sciences and humanities. In this special section, we attempt to bring cross-disciplinary conversations among ecology, evolution, behavior, and genetics on the one hand and critical perspectives from the social sciences and humanities on the other into the pages—and in front of the readers—of a scientific journal. In this introduction to the special section, we recount and reflect on the …

A Haploid Pseudo-Chromosome Genome Assembly For A Keystone Sagebrush Species Of Western North American Rangelands, Anthony E. Melton, Richard S. Beard Jr., Carlos Dave C. Dumaguit, Jennifer S. Forbey, Marie-Anne De Graaff, Peggy Martinez, Stephen J. Novak, Desiree Self, Marcelo Serpe, Sven Buerki

Biology Faculty Publications and Presentations

Increased ecological disturbances, species invasions, and climate change are creating severe conservation problems for several plant species that are widespread and foundational. Understanding the genetic diversity of these species and how it relates to adaptation to these stressors are necessary for guiding conservation and restoration efforts. This need is particularly acute for big sagebrush (Artemisia tridentata; Asteraceae), which was once the dominant shrub over 1,000,000 km² in western North America but has since retracted by half and thus has become the target of one of the largest restoration seeding efforts globally. Here, we present the first reference-quality …

The Effects Of Genotype And Nutrient Conditions On Poplar Growth And Physiology, Bethanie Natalie

Theses - ALL

The United States is currently reliant on other nations for energy and has been unable tomeet goals set for domestic production of 'green' energy such as second generation biofuel. Overcoming these challenges requires examination of transgenic biofuel feedstock plants, specifically in terms of their responses to two nutrient treatments. Here, we investigated the photosynthetic and growth impacts of expressing a hyperthermophilic endoglucanase in planta in a wildtype (WT) Populus alba × grandidentata background under 100% and 15% strength nutrient treatments. This gene was inserted for improvement of the pretreatment process and to limit inputs required during biofuel production. We characterized …

Near-Infrared Spectroscopy Aids Ecological Restoration By Classifying Variation Of Taxonomy And Phenology Of A Native Shrub, Brecken C. Robb, Peter J. Olsoy, T. Trevor Caughlin, Stephanie J. Galla, Marcella R. Fremgen-Tarantino, Jordan D. Nobler, Jennifer S. Forbey

Biology Faculty Publications and Presentations

Plant communities are composed of complex phenotypes that not only differ among taxonomic groups and habitats but also change over time within a species. Restoration projects (e.g. translocations and reseeding) can introduce new functional variation in plants, which further diversifies phenotypes and complicates our ability to identify locally adaptive phenotypes for future restoration. Near-infrared spectroscopy (NIRS) offers one approach to detect the chemical phenotypes that differentiate plant species, populations, and phenological states of individual plants over time. We use sagebrush (Artemisia spp.) as a case study to test the accuracy by which NIRS can classify variation within taxonomy and …

Distribution Of Carrion-Associated Beetles And Their Phoretic Mites Along An Urban-Rural Gradient In Northeast Alabama, Kennedy Norris

Theses

Global insect decline has been linked to urbanization, most notably by habitat fragmentation. These insects perform important ecological functions such as pollination, managing pests, and decomposing carrion to recycle nutrients back into the environment. Despite the importance of nutrient recycling behavior displayed by carrion-associated beetles, little research has been done on them in the southeastern US. Previous studies have found a relationship between urbanization, less favorable environmental conditions, carrion availability, and decreased insect diversity. However, no studies have been conducted in the southeastern United States on the relationship of these beetles to their environment despite having the highest rates of …