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Intermediates In Sars-Cov-2 Spike–Mediated Cell Entry, Tara C. Marcink, Thomas Kicmal, Emily Armbruster, Zhening Zhang, Gillian Zipursky, Kate L. Golub, Mohab Idris, Jonathan Khao, Jennifer Drew-Bear, Gael Mcgill, Tom Gallagher, Matteo Porotto, Amédée Des Georges, Anne Moscona Aug 2022

Intermediates In Sars-Cov-2 Spike–Mediated Cell Entry, Tara C. Marcink, Thomas Kicmal, Emily Armbruster, Zhening Zhang, Gillian Zipursky, Kate L. Golub, Mohab Idris, Jonathan Khao, Jennifer Drew-Bear, Gael Mcgill, Tom Gallagher, Matteo Porotto, Amédée Des Georges, Anne Moscona

Advanced Science Research Center

SARS-CoV-2 cell entry is completed after viral spike (S) protein–mediated membrane fusion between viral and host cell membranes. Stable prefusion and postfusion S structures have been resolved by cryo–electron microscopy and cryo–electron tomography, but the refolding intermediates on the fusion pathway are transient and have not been examined. We used an antiviral lipopeptide entry inhibitor to arrest S protein refolding and thereby capture intermediates as S proteins interact with hACE2 and fusion-activating proteases on cell-derived target membranes. Cryo–electron tomography imaged both extended and partially folded intermediate states of S2, as well as a novel late-stage conformation on the pathway to …

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Computational Study On The Effectiveness Of Flavonoids From Marsilea Crenata C. Presl As Potent Sirt1 Activators And Nfκb Inhibitors, Sri Rahayu, Sasangka Prasetyawan, Sri Widyarti, Mochammad Fitri Atho’Illah, Gatot Ciptadi Aug 2022

Computational Study On The Effectiveness Of Flavonoids From Marsilea Crenata C. Presl As Potent Sirt1 Activators And Nfκb Inhibitors, Sri Rahayu, Sasangka Prasetyawan, Sri Widyarti, Mochammad Fitri Atho’Illah, Gatot Ciptadi

Karbala International Journal of Modern Science

Ovarian aging is a natural process in females, and it occurs due to an elevated ROS-induced inflammation caused by oxidative stress. SIRT-1 is a metabolic sensor that tightly regulates oxidative and inflammatory responses. However, this regulative function is antagonized by NFκB. Therefore, the objective of this study was to explore the pathways involved in aging and identify the flavonoid compounds from Marsilea crenata that might be useful as SIRT1 activators and NFκB inhibitors. The screening began with exploring the protein-protein interaction in the experimental process using BioGrid, and the role of the flavonoid was evaluated using STITCH. The interaction between …

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Molecular Characterization Of Esbls And Ampc Β-Lactamases In Bacteria Isolated From Currency Notes Circulating In Mosul City, Iraq, Mahmood Zeki Al-Hasso, Shakir Ghazi Gergees, Zahraa Khairialdeen Mohialdeen Aug 2022

Molecular Characterization Of Esbls And Ampc Β-Lactamases In Bacteria Isolated From Currency Notes Circulating In Mosul City, Iraq, Mahmood Zeki Al-Hasso, Shakir Ghazi Gergees, Zahraa Khairialdeen Mohialdeen

Karbala International Journal of Modern Science

The Iraqi currency notes circulating in Mosul city were evaluated for the occurrence of ESBLs and AmpC b-lactamaseproducing bacteria. Four hundred and twenty-two Gram-positive and negative bacterial isolates with different antimicrobial resistance profiles were recovered from 250 samples collected during the period from April to July 2021, among which 150 isolates (35.5%) were multi-drug resistant (MDR). The study found that 16.4% and 14.8% of Gram negative isolates were positive for ESBLs and AmpC phenotypic detection tests, respectively. Interestingly, 6.6% of the isolates were simultaneously positive for both tests. Molecular characterization was carried out using PCR technique to determine the prevalent …

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Molecular Docking And Dynamics Simulation Studies To Predict Multiple Medicinal Plants’ Bioactive Compounds Interaction And Its Behavior On The Surface Of Denv-2 E Protein, Arief Hidayatullah, Wira Eka Putra, Muhaimin Rifa’I, Sustiprijatno Sustiprijatno, Diana Widiastuti, Muhammad Fikri Heikal, Hendra Susanto, Wa Ode Salma, Hilal Mulyadi Aug 2022

Molecular Docking And Dynamics Simulation Studies To Predict Multiple Medicinal Plants’ Bioactive Compounds Interaction And Its Behavior On The Surface Of Denv-2 E Protein, Arief Hidayatullah, Wira Eka Putra, Muhaimin Rifa’I, Sustiprijatno Sustiprijatno, Diana Widiastuti, Muhammad Fikri Heikal, Hendra Susanto, Wa Ode Salma, Hilal Mulyadi

Karbala International Journal of Modern Science

The envelope protein (E) is a fusion class II protein that is essential for DENV fusion. We use two active compounds derived from commonly used plants in Indonesia: galangin and kaempferide. We ran a docking and 1000 ps molecular dynamic analysis with normal physiological parameters. During the simulation, galangin and kaempferide binding sites fluctuated. But chloroquine has lesser ligand mobility, hence keeping contact with fusion loops, whereas both drugs lose contact with hydrophobic pockets. However, the two active compounds have a more stable ligand configuration. Less than 2 Å alterations were seen in the RMSF simulation of the protein E …

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Microbial Dimension Of Symbiosis And Dysbiosis In Fungus–Gardening Ants, Blake S. Bringhurst Aug 2022

Microbial Dimension Of Symbiosis And Dysbiosis In Fungus–Gardening Ants, Blake S. Bringhurst

Biology Theses

With symbiotic partners, the means by which a host obtain a symbiont influence the specificity between the host and the symbiont. One such system where symbiont specificity is seen to varying degrees is with higher attine ants. Higher attine ants have symbiotic relationships with the fungal cultivar they grow and the bacterial symbionts in their microbiomes. Among the higher attines, there are two broad groups of fungi. Most ants in the genus Trachymyrmex tend to grow Clade–B fungi, which are a group of undescribed Leucocoprinus species, while leaf–cutting ants in the genera Acromyrmex and Atta tend to grow Clade–A fungi …

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Diversity Of Bees In Trees On An Urban College Campus, Aidan Castricone Aug 2022

Diversity Of Bees In Trees On An Urban College Campus, Aidan Castricone

Biology Student Scholarship

It is well known that insect pollinator populations are declining and a lack of nutrients (i.e flowering plants) may be a contributor to this decline. Insect pollinators forage for pollen and nectar, which provides proteins, fats, and carbohydrates, from flowering plants. Current research on pollinator foraging behavior focuses on pollinators that forage on the ground thus overlooking those that forage in trees. Trees such as Kousa dogwood (Cornus kousa) and crabapple (Malus sp.) bloom in early Spring, even before herbaceous perennials and forbs. Thus trees are likely the only places for early flying pollinators to find food. …

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Assessment Of Population Size, Survival, And Movement Behavior Of The Rare Frosted Elfin Butterfly, Breelyn Gilbert Aug 2022

Assessment Of Population Size, Survival, And Movement Behavior Of The Rare Frosted Elfin Butterfly, Breelyn Gilbert

Biology Student Scholarship

The frosted elfin butterfly (Callophrys irus) is a locally rare Lycaenaid with spotty distribution throughout the eastern United States. As host plant specialists, frosted elfins only lay eggs on two plant species. Yellow wild indigo (Baptisia tinctoria), one of the host plants for this species, is found in abundance at Gavins Pond (Foxboro MA). Since 2000, Massachusetts Butterfly Club members have been informally monitoring the frosted elfin population at this site; however, no systematic population surveys have been done. Using their data, I estimated annual population size from 2000 to 2022. In 2022, I also performed …

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Floral Nutritional Value And Plant-Pollinator Interactions In Providence, Rhode Island, Gracey Sorensen Aug 2022

Floral Nutritional Value And Plant-Pollinator Interactions In Providence, Rhode Island, Gracey Sorensen

Biology Student Scholarship

Bees obtain nutrients from flowers. Pollen provides proteins and fats; nectar provides carbohydrates and some amino acids. With recent decline in pollinator populations, conservationists are interested in providing nutritious plants for pollinators. Counterintuitively, urban areas have the green space for pollinator friendly plantings. On Providence College’s urban campus, I examined how floral abundance and pollinator interactions correlate to the nutritional value of plant species. Quadrat surveys were performed within 53 quadrats through the Spring and Summer for plant-pollinator interactions. Pollen was collected from all flowering species and analyzed for nitrogen and carbon content using an elemental analyzer. Nectar samples were …

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The Effects Of Prescribed Fire On Ant-Mediated Seed Dispersal In Missouri, Eva M. Colberg Aug 2022

The Effects Of Prescribed Fire On Ant-Mediated Seed Dispersal In Missouri, Eva M. Colberg

Dissertations

Many aspects of animal-mediated seed dispersal are vulnerable to disturbance, including partner identity and dispersal quantity and quality. This dissertation explores ant-mediated seed dispersal of the herb Sanguinaria canadensis in Missouri Ozark oak forests, where prescribed fire is a common land management tool. In Chapter 1, I test the definition of a keystone seed disperser using absolute and relative contributions of different ant species to the quantity and distance of seed dispersal, based on field observations of S. canadensis seed dispersal. I demonstrate that the ant Aphaenogaster rudis is better described as a numerically dominant rather than keystone seed disperser, …

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Complete Genome Sequences Of Two Temperate Bacillus Subtilis Phages Isolated At Tumamoc Hill Desert Laboratory, Gregory P. Krukonis, Amanda K. Kemp, Katie F. Storrie, Vivian R. Chavira, Hayden W. Lantrip, Victoria D. Perez, Desiree A. Reyes, Julian A. Truax, Rachel Loney, Veronique A. Delesalle Aug 2022

Complete Genome Sequences Of Two Temperate Bacillus Subtilis Phages Isolated At Tumamoc Hill Desert Laboratory, Gregory P. Krukonis, Amanda K. Kemp, Katie F. Storrie, Vivian R. Chavira, Hayden W. Lantrip, Victoria D. Perez, Desiree A. Reyes, Julian A. Truax, Rachel Loney, Veronique A. Delesalle

Biology Faculty Publications

Bacteriophages are important in structuring bacterial communities, including desert soils dominated by Bacillus species. Here, we describe two genetically similar temperate phages isolated on a Bacillus subtilis strain from soil in Tucson, Arizona. Their double-stranded DNA (dsDNA) genomes contain 98 and 102 genes, with a set of 4 genes being found in only one phage.

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Developmental Basis Of Evolutionary Lung Loss In Plethodontid Salamanders, Zachary R. Lewis, Ryan R. Kerney, James Hanken Aug 2022

Developmental Basis Of Evolutionary Lung Loss In Plethodontid Salamanders, Zachary R. Lewis, Ryan R. Kerney, James Hanken

Biology Faculty Publications

One or more members of four living amphibian clades have independently dispensed with pulmonary respiration and lack lungs, but little is known of the developmental basis of lung loss in any taxon. We use morphological, molecular, and experimental approaches to examine the Plethodontidae, a dominant family of salamanders, all of which are lungless as adults. We confirm an early anecdotal report that plethodontids complete early stages of lung morphogenesis: Transient embryonic lung primordia form but regress by apoptosis before hatching. Initiation of pulmonary development coincides with expression of the lung-specification gene Wnt2b in adjacent mesoderm, and the lung rudiment expresses …

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All Is Perception, Robert Friedman Aug 2022

All Is Perception, Robert Friedman

Faculty Publications

This editorial addresses the universality and importance of the science of perception. In particular, recently published studies in this journal illustrate the natural variations in perception. These articles are a reminder of perception as a natural process with inherent variations and that any two individuals are not guaranteed to form the same representation of an object, regardless of whether it originates from the senses or not. Since perception is a foundation for higher cognition, it also has an immense influence on studies of humanity and interpretations of natural processes.

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Dynamic Energy Budget Models: Fertile Ground For Understanding Resource Allocation In Plants In A Changing World, Sabrina E. Russo, Glenn Ledder, Erik B. Muller, Roger M. Nisbet Aug 2022

Dynamic Energy Budget Models: Fertile Ground For Understanding Resource Allocation In Plants In A Changing World, Sabrina E. Russo, Glenn Ledder, Erik B. Muller, Roger M. Nisbet

School of Biological Sciences: Faculty Publications

Climate change is having dramatic effects on the diversity and distribution of species. Many of these effects are mediated by how an organism’s physiological patterns of resource allocation translate into fitness through effects on growth, survival and reproduction. Empirically, resource allocation is challenging to measure directly and so has often been approached using mathematical models, such as Dynamic Energy Budget (DEB) models. The fact that all plants require a very similar set of exogenous resources, namely light, water and nutrients, integrates well with the DEB framework in which a small number of variables and processes linked through pathways represent an …

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Defence Mitigation By Predators Of Chemically Defended Prey Integrated Over The Predation Sequence And Across Biological Levels With A Focus On Cardiotonic Steroids, Shabnam Mohammadi, Lu Yang, Matthew Bulbert, Hannah M. Rowland Aug 2022

Defence Mitigation By Predators Of Chemically Defended Prey Integrated Over The Predation Sequence And Across Biological Levels With A Focus On Cardiotonic Steroids, Shabnam Mohammadi, Lu Yang, Matthew Bulbert, Hannah M. Rowland

School of Biological Sciences: Faculty Publications

Predator–prey interactions have long served as models for the investigation of adaptation and fitness in natural environments. Anti-predator defences such as mimicry and camouflage provide some of the best examples of evolution. Predators, in turn, have evolved sensory systems, cognitive abilities and physiological resistance to prey defences. In contrast to prey defences which have been reviewed extensively, the evolution of predator counter-strategies has received less attention. To gain a comprehensive view of how prey defences can influence the evolution of predator counter-strategies, it is essential to investigate how and when selection can operate. In this review we evaluate how predators …

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Cyclase-Associated Protein 1 (Cap1) In Cyclic Adenosine Monophosphate (Camp) And Protein Kinase C (Pkc) Signals To Regulate Cancer Cell Functions, Lokesh Kalki Mani Sai Akana Aug 2022

Cyclase-Associated Protein 1 (Cap1) In Cyclic Adenosine Monophosphate (Camp) And Protein Kinase C (Pkc) Signals To Regulate Cancer Cell Functions, Lokesh Kalki Mani Sai Akana

Student Theses and Dissertations

Cyclase-Associated Protein 1 (CAP1) is an actin-regulating protein that promotes actin dynamics and is also involved in cell adhesion and proliferation as well as implicated in human cancers. We previously found that phosphorylation at the Ser307/Ser309 tandem regulatory site on CAP1 is critical for CAP1 functions in breast and pancreatic cancer cells. We silenced CAP1 through RNAi in HCT116 and SW480 colon cancer cells as confirmed in Western blotting. We have found that depletion of CAP1 led to reduced FAK (Focal Adhesion Kinase) activity and matrix adhesion in SW480 cells, while it stimulated FAK activity and cell adhesion in HCT116 …

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Targeting The Maladaptive Effects Of Binge Drinking On Circadian Gene Expression, Courtney Ledford Aug 2022

Targeting The Maladaptive Effects Of Binge Drinking On Circadian Gene Expression, Courtney Ledford

University Honors Theses

Previous studies 1) support a role of circadian genes in regulating alcohol intake, and 2) reveal that harmful alcohol use alters circadian rhythms. However, there [is] minimal knowledge of the effects of chronic alcohol processes on rhythmic circadian gene expression across brain regions important for circadian biology and alcohol intake. Therefore, the present study sought to test the effects of chronic binge-like drinking on diurnal circadian gene rhythms in the master circadian pacemaker (SCN), the ventral tegmental area (VTA), and the nucleus accumbens (NAc) in High Drinking in the Dark-1 (HDID-1) mice, a unique genetic risk model for drinking to …

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The Timing And Development Of Infections In A Fish-Cestode Host-Parasite System, Anika M. Wohlleben, Natalie C. Steinel, Néva P. Meyer, John A. Baker, Susan A. Foster Aug 2022

The Timing And Development Of Infections In A Fish-Cestode Host-Parasite System, Anika M. Wohlleben, Natalie C. Steinel, Néva P. Meyer, John A. Baker, Susan A. Foster

Biology

The cestode Schistocephalus solidus is a common parasite in freshwater threespine stickleback populations, imposing strong fitness costs on their hosts. Given this, it is surprising how little is known about the timing and development of infections in natural stickleback populations. Previous work showed that young-of-year stickleback can get infected shortly after hatching. We extended this observation by comparing infection prevalence of young-of-year stickleback from 3 Alaskan populations (Walby, Cornelius and Wolf lakes) over 2 successive cohorts (2018/19 and 2019/20). We observed strong variation between sampling years (2018 vs 2019 vs 2020), stickleback age groups (young-of-year vs 1-year-old) and sampling populations.

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Constraints On The Evolution Of Toxin-Resistant Na,K-Atpases Have Limited Dependence On Sequence Divergence, Shabnam Mohammadi, Santiago Herrera-Álvarez, Lu Yang, María Del Pilar Rodrı´Guez-Ordoñez, Karen Zhang, Jay F. Storz, Susanne Dobler, Andrew J. Crawford, Peter Andolfatto Aug 2022

Constraints On The Evolution Of Toxin-Resistant Na,K-Atpases Have Limited Dependence On Sequence Divergence, Shabnam Mohammadi, Santiago Herrera-Álvarez, Lu Yang, María Del Pilar Rodrı´Guez-Ordoñez, Karen Zhang, Jay F. Storz, Susanne Dobler, Andrew J. Crawford, Peter Andolfatto

School of Biological Sciences: Faculty Publications

A growing body of theoretical and experimental evidence suggests that intramolecular epistasis is a major determinant of rates and patterns of protein evolution and imposes a substantial constraint on the evolution of novel protein functions. Here, we examine the role of intramolecular epistasis in the recurrent evolution of resistance to cardiotonic steroids (CTS) across tetrapods, which occurs via specific amino acid substitutions to the α-subunit family of Na,K-ATPases (ATP1A). After identifying a series of recurrent substitutions at two key sites of ATP1A that are predicted to confer CTS resistance in diverse tetrapods, we then performed protein engineering experiments to test …

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An Ecological And Physiological Assessment Of Feather Mite-Bird Symbioses, Kevin James Krajcir Aug 2022

An Ecological And Physiological Assessment Of Feather Mite-Bird Symbioses, Kevin James Krajcir

Student Theses and Dissertations

Feather mites are ectosymbiotic arthropods that reside primarily on the wing and tail feathers of most birds. Despite our growing understanding of feather mite biology, previous studies of the nature of the feather mite-avian host relationship report mutualism, parasitism, and commensalism. Such inconsistencies are likely due to narrow study designs that fail to capture the possibility of context dependency in this interaction. To provide some clarity surrounding the nature of this symbiotic system, we explored how geographical, climatic, and ecological contexts relate to variation in feather mite abundance and how that abundance, consequently, associates with multiple metrics of host condition …

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Tfa Inference: Using Mathematical Modeling Of Gene Expression Data To Infer The Activity Of Transcription Factors, Cynthia Ma Aug 2022

Tfa Inference: Using Mathematical Modeling Of Gene Expression Data To Infer The Activity Of Transcription Factors, Cynthia Ma

McKelvey School of Engineering Graduate Student Theses & Dissertations

Transcription factors (TFs) are a set of proteins that play a key role in the information processing system that enables a cell to respond to changes in internal and external state. By binding near a gene in a cell’s DNA, a TF can influence that gene’s expression level, triggering the appropriate increase or decrease in production levels of proteins that are needed to handle stressors like a change in nutrient availability or damage to the cell’s internal structures. Transcription factor activity (TFA) is a measure of how much effect a TF has on its target genes in a given sample …

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Machine Learning And Scalable Informatics Methods To Predict Disease Status From Multimodal Biomedical Data, Hossein Mohammadian Foroushani Aug 2022

Machine Learning And Scalable Informatics Methods To Predict Disease Status From Multimodal Biomedical Data, Hossein Mohammadian Foroushani

McKelvey School of Engineering Graduate Student Theses & Dissertations

Biological understanding of complex diseases such as stroke and obesity is critical for the advancement of medicine. Further knowledge discovery can provide effective biomarkers to improve disease diagnosis and prognosis, identify driver mutations, predict individual genetic susceptibility for early prevention and effective disease management, and facilitate development of personalized drugs. Stroke is the second leading cause of death and long-term disability in the world. Thus, stroke management is a time-sensitive emergency. The initial hours after stroke onset map the trajectory of subsequent neurologic complications. Cerebral edema develops hours to days after acute ischemic stroke and may result in midline shift …

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Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler Aug 2022

Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Biological rhythms mediate important within-host processes such as metabolism, immunity, and behavior which are often linked to combating disease exposure. For many hosts, exposure to pathogens occurs while feeding. However, the link between feeding rhythms and infection risk is unclear because feeding behavior is tightly coupled with immune and metabolic processes which may decrease susceptibility to infection. Here, we use the Daphnia dentifera–Metschnikowia bicuspidata host–pathogen system to determine how rhythms in feeding rate and immune function mediate infection risk. The host is known to have a nocturnal circadian rhythm in feeding rate, yet we found that they do not exhibit …

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Foraging Habitat Selection Of Shrubland Bird Community In Tropical Dry Forest, Anant Deshwal, Steven L. Stephenson, Pooja Panwar, Brett A. Degregorio, Ragupathy Kannan, John D. Willson Aug 2022

Foraging Habitat Selection Of Shrubland Bird Community In Tropical Dry Forest, Anant Deshwal, Steven L. Stephenson, Pooja Panwar, Brett A. Degregorio, Ragupathy Kannan, John D. Willson

Biological Sciences Faculty Publications and Presentations

Habitat loss due to increasing anthropogenic disturbance is the major driver for bird population declines across the globe. Within the Eastern Ghats of India, shrubland bird communities are threatened by shrinking of suitable habitats due to increased anthropogenic disturbance and climate change. The development of an effective habitat management strategy is hampered by the absence of data for this bird community. To address this knowledge gap, we examined foraging sites for 14 shrubland bird species, including three declining species, in three study areas representing the shrubland type of forest community in the Eastern Ghats. We recorded microhabitat features within an …

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Ewing Sarcoma Transcriptome: Identification Of Foxn3 As A Novel Target Of Ews-Fli1, Natasha Kreiling Aug 2022

Ewing Sarcoma Transcriptome: Identification Of Foxn3 As A Novel Target Of Ews-Fli1, Natasha Kreiling

Theses & Dissertations

Ewing Sarcoma is an aggressive cancer characterized by a fusion gene formed from a chromosomal translocation between a RNA binding protein and a transcription factor. The most common fusion gene is EWS-FLI1 a potent oncogenic transcription factor responsible for initiating genome instability. EWS-FLI1 promotes tumor growth by dysregulating several genes and pathways. We have identified FOXN3, an important gene in cell cycle control and development, as being suppressed by EWS-FLI1. In the future, a better understanding of FOXN3 and its ability to act as a tumor suppressor in EWS will unravel novel mechanisms of EWS pathogenesis, potential biomarker and drug …

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Behavioral Responses Of A Parasitoid Fly To Rapidly Evolving Host Signals, E. Dale Broder, James H. Gallagher, Aaron W. Wikle, Cameron P. Venable, David M. Zonana, Spencer J. Ingley, Tanner C. Smith, Robin M. Tinghitella Aug 2022

Behavioral Responses Of A Parasitoid Fly To Rapidly Evolving Host Signals, E. Dale Broder, James H. Gallagher, Aaron W. Wikle, Cameron P. Venable, David M. Zonana, Spencer J. Ingley, Tanner C. Smith, Robin M. Tinghitella

Biological Sciences: Faculty Scholarship

Animals eavesdrop on signals and cues generated by prey, predators, hosts, parasites, competing species, and conspecifics, and the conspicuousness of sexual signals makes them particularly susceptible. Yet, when sexual signals evolve, most attention is paid to impacts on intended receivers (potential mates) rather than fitness consequences for eavesdroppers. Using the rapidly evolving interaction between the Pacific field cricket, Teleogryllus oceanicus, and the parasitoid fly, Ormia ochracea, we asked how parasitoids initially respond to novel changes in host signals. We recently discovered a novel sexual signal, purring song, in Hawaiian populations of T. oceanicus that appears to have evolved because it …

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Purifications Of Iraqi Petroleum Using Ceramic Ball Nano Cobalt Nickel Ferrite Filter, Huda Jabbar, Enas Muhi, Tahseen Hussien Aug 2022

Purifications Of Iraqi Petroleum Using Ceramic Ball Nano Cobalt Nickel Ferrite Filter, Huda Jabbar, Enas Muhi, Tahseen Hussien

Karbala International Journal of Modern Science

Iraqi petroleum, especially from the Al-Ahdab, has a big problem resulting from its high percentage of heavy metals. In this paper, heavy metals were reduced or removed from Iraqi petroleum using a Ceramic Ball Nano Cobalt Nickel Ferrite Filter (BCNF), synthesized by combining kaolin and palm frond in a 30% ratio with Co0.8Ni0.2Fe2O4 nanoparticles in a various ratios (5, 10, 15, and 20%). The sol-gel technique prepared Co0.8Ni0.2Fe2O4 nanoparticles. The structure and magnetic properties of the material are described using X-RD, FT-IR, and VSM techniques. In addition, the water ab-sorption ratio and apparent porosity were assessed. The results show that …

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Studying The Physical And Biological Characteristics Of Denture Base Resin Pmma Reinforced With Zro2 And Tio2 Nanoparticles, Fatin A. Asim, Entessar H.A. Al-Mosaweb, Wafaa A. Hussain Aug 2022

Studying The Physical And Biological Characteristics Of Denture Base Resin Pmma Reinforced With Zro2 And Tio2 Nanoparticles, Fatin A. Asim, Entessar H.A. Al-Mosaweb, Wafaa A. Hussain

Karbala International Journal of Modern Science

Polymethyl methacrylate (PMMA) suffers from poor mechanical properties that limit its application in the bio-medical field. In this study, PMMA was reinforced with zirconium dioxide (ZrO2) and titanium dioxide (TiO2) nanopar-ticles; subsequently, the hardness, porosity, biocompatibility, bacterial adhesion, and colonization of the reinforced PMMA with various oxide nanoparticles were characterized. The results of this study indicated that reinforced material inhibits bacterial growth and decreases bacterial adhesion by decreasing porosity and increasing PMMA hardness. Based on the findings, 3 wt% PMMA-ZrO2 and 3 wt% PMMA-ZrO2 -TiO2 composites significantly inhibited bacterial growth and adherence while maintaining hemolysis PT and INR and enhancing …

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Skin Lesion Segmentation Based On U-Shaped Network, Muna Khalaf, Ban N. Dhannoon Aug 2022

Skin Lesion Segmentation Based On U-Shaped Network, Muna Khalaf, Ban N. Dhannoon

Karbala International Journal of Modern Science

Skin lesion segmentation is an essential step toward accurate skin lesion diagnosis. The need to automate Skin lesion segmentation on the one hand, and the challenges it faces, on the other hand, have made it a growing area of research and focus. Automation of skin lesion segmentation helps reduce the effort and time needed for diagnosis and treatment and helps make better utilization of available data and shared experiences. The challenges faced by the automation of skin lesion segmentation can be broadly defined by (but not limited to); variations in texture, shape, and size for skin lesions and the low …

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C60 Hydrofullerene Induced Autophagy And Ameliorated Gfap In H2o2 Treated Human Malignant Glioblastoma U-373 Cell Line, Aryan M. Faraj, Can A. Agca, Victor S. Nedzvetsky, Artem A. Tykhomyrov Aug 2022

C60 Hydrofullerene Induced Autophagy And Ameliorated Gfap In H2o2 Treated Human Malignant Glioblastoma U-373 Cell Line, Aryan M. Faraj, Can A. Agca, Victor S. Nedzvetsky, Artem A. Tykhomyrov

Karbala International Journal of Modern Science

Glioblastoma is one of the most combative astrocytoma that is resistant to chemotherapy and radiotherapy. This resistance makes it very difficult to treat. However, researches have shown that nanoparticles especially C60 hydrofullerene have antioxidant and anticancer activity. The effect of C60 hydrofullerene in cancer has been extensively studied; however, the potential regulation of autophagy and modulation of the Glial Fibrillary Acidic Protein (GFAP) gene has not been addressed in glioblastomas. Glioblastoma U-373 cell was treated with 0.5 µM of C60 hydrofullerene and/or 1 mM of hydrogen peroxide (H2O2) for 24 hours. This study demonstrated that C60 hydrofullerene and H2O2 significantly …

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Improving Prediction Of Arabic Fake News Using Fuzzy Logic And Modified Random Forest Model, Tahseen A. Wotaifi, Ban N. Dhannoon Aug 2022

Improving Prediction Of Arabic Fake News Using Fuzzy Logic And Modified Random Forest Model, Tahseen A. Wotaifi, Ban N. Dhannoon

Karbala International Journal of Modern Science

Throughout the last few years, the world is witnessing the so-called age of social media, as there is a complete dependence on these sites for following up on events and activities. The problem is that the misinformation or fake news is always released at the appropriate time, so this false news spreads quickly and takes a very wide resonance. Although several studies are performed to determine English fake news, the identification of Arabic misinformation remains underdeveloped. This study aims to build an improved learning model for detecting fake news in the Arabic language. Unlike previous studies that depended on analyzing …

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