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Does Stress Physiology Mediate Disease Resistance In The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum)?, Megan Zerger

2023 Midwest Ecology & Evolution Conference

Amphibian biodiversity has greatly diminished in recent years due to disease. Panzootic pathogenic fungi Batrachochytrium dendrobatidis (Bd) causes the deadly disease chytridiomycosis (chytrid), a primary driver of catastrophic global amphibian declines and extinctions. The pathogenesis of chytrid is still unclear, as certain species and individuals within a species are differentially affected. Susceptibility and mortality of Bd are influenced by prolonged chronic stress. Prolonged glucocorticoid activity deleteriously affects many of the same physiological processes as Bd infections, and corticosterone is the primary glucocorticoid released by amphibians in response to stress. Thus, the objective of our study is to assess …

Changes In Aboveground Biomass Of Ammophila Breviligulata Are Influenced By Disturbance Along A Climatic Gradient., Vincent Cafazo

2023 Midwest Ecology & Evolution Conference

Coastal dunes are important geographic features that protect shorelines from erosion while supporting unique plant and animal biodiversity. The ecosystem engineering plant Ammophila breviligulata is particularly important for coastal dune development because of its ability to trap sediment and build up dunes. Understanding how environment conditions affect functional traits of this plant is an important first step towards protecting coastal dunes and improving their conservation. Our hypothesis in this study was that we would see a decrease in aboveground functional traits of A. breviligulata with increasing climatic and nutrient stress. In July 2022, we identified four different sites along the …

Trait Variation In Bryophytes At Karst Cave Entrances, Chenoa Briggs

2023 Midwest Ecology & Evolution Conference

Cave entrances are critical ecosystems in their role as ecotones between the interior cave environment and the surrounding external environment. Bryophytes are highly sensitive to environmental changes and many species found at caves are specialists to those habitats. This study aims to identify how functional traits of bryophytes are affected by the cave environment. Bryophytes and environmental variables were sampled from six cave entrances. Functional trait data was collected from literature. The data from this study supplies information on the functional traits of bryophytes and can provide insight into the effects of environmental pressures on community assembly.

Seroton-Ing Down The Stress: Serotonergic Activity In Response To Predators In Threespined Stickleback (Gasterosteus Aculeatus), Michaela M. Rogers

2023 Midwest Ecology & Evolution Conference

Animals constantly face stress in the environment due to rapid human-induced changes. A strong interest lies in the evolution of coping mechanisms that can help organisms deal with and respond to these stressors. Serotonin is a neurotransmitter that is highly conserved across taxonomic groups and is upregulated in response to a broad range of stressors, including predator exposure. Serotonin upregulation can help organisms cope with the threat of a predator by decreasing fear and anxiety. Previous studies have shown an increase in serotonergic activity following acute predator exposures in mice, rats, and fish after only one exposure, but less is …

Assessing The Health And Behavioral Effects Of Turtle Racing On Eastern Box Turtles (Terrapene Carolina), Michelle K. Weaver

2023 Midwest Ecology & Evolution Conference

Turtle racing involves the public capturing largely wild-caught box turtles (Terrapene spp.) and racing them at standalone events or at races incorporated into larger family events (e.g., festivals). These turtle races are legal in most states because of a lack of regulations and/or funding to enforce regulations that may exist. Moreover, we lack information on the potential effects (e.g., disease transmission, survival, movements, and general health) of races on the turtles that could help support the implementation of regulations. Our objective was to understand the short-term and long-term health and behavioral effects of turtle races on eastern box turtles (Terrapene …

Late Cretaceous Marine Vertebrates From The Tarrant Formation, Tarrant County, Texas U.S.A, Aaron Johnson, Kenshu Shimada

2023 Midwest Ecology & Evolution Conference

The Tarrant Formation (middle Cenomanian: ca. 96 million years ago) is a stratigraphic member of the Upper Cretaceous Eagle Ford Group in Texas, U.S.A., and consists of calcareous sandstone formed near the East Texas Embayment of the Late Cretaceous Western Interior Seaway, an epicontinental seaway in North America. We examined vertebrate fossils from the Tarrant Formation based on specimens collected through surface collecting and bulk sampling from three localities in Tarrant County, Texas, including the type locality of a pachyrhizodontid fish, Polcynichthys lloydhilli. Our preliminary examination shows that the fossil fauna includes shark species such as Ptychodus anonymous, Archaeolamna sp., …

Divergent Patterns Of Belowground Allocation In Response To Stress In A Key Coastal Grass, Shannon Walker

2023 Midwest Ecology & Evolution Conference

Environmental stressors shape life history strategies and biodiversity across the globe. Stress is particularly important in coastal sand dune habitats where nutrients and water are extremely limiting. However human-driven nutrient enrichment and climate change may alter these factors in sand dune habitats, with cascading effects on plant communities and ecosystem functioning. In 2022, we established monitoring plots in four sand dune sites along the western Michigan coast to examine the impacts of climatic and nutrient stress on vegetation. These sites spanned an increasing gradient of climatic and nutrient stress from south to north. We hypothesized that increasing climatic and nutrient …

Habitiat Structure And Ecomorphology Of Fishes In Neotropical Blackwater Creeks., Devya Hemraj-Naraine

2023 Midwest Ecology & Evolution Conference

Freshwater ecosystems provide habitat for a diversity of aquatic flora and fauna. However, anthropogenic activities like mining and pollution alters aquatic habitats and the ecosystem services they provide. In South America, which hosts one quarter of all freshwater fish species, aquatic habitats are under considerable encroachment from human development. We surveyed nine blackwater creeks tributary to the Demarara River along a major Highway in Guyana to determine whether fish morphology and community structure correlate with habitat characteristics among sites with different degrees of human impact. Twenty-three morphological traits were measured on 3-5 adult specimens of each species. The most abundant …

Repeatability And Distribution Of Behavior Types Over Time And Across Populations In Free-Ranging Adult And Juvenile Ornate Box Turtles (Terrapene Ornata), Abigail Trautman

2023 Midwest Ecology & Evolution Conference

As studies continue to investigate inter-individual variation, it is becoming increasingly evident that individual animals have their own unique behavior type (personality; repeatable behaviors over time and context) that persists throughout their life. Behavior is simply different forms of motion, and a behavior type influences which motions (behaviors) are most likely to be on display. Behavioral types can influence resource acquisition, predation risk, and mating opportunities and thus can directly influence the fitness of any given individual. In this study, we examine the behavior types of free-ranging adult ornate box turtles (Terrapene ornata), including their repeatability and variation …

Forest Recruitment Dynamics Within The Southern Boreal-Temperate Ecotone, Kiley M. Chernicky

2023 Midwest Ecology & Evolution Conference

Understanding how global climate change will alter future forest composition is especially challenging in forest communities where tree species have long co-occurred, but their competitive balance may be altered due to changes in climate. Of particular concern with increasing global temperatures are boreal forest communities that lie on the southern boreal-temperate forest ecotone, where the boreal forest is particularly vulnerable. The aim of my research is to investigate shifts in recruitment dynamics due to climate change near the southern range limit of two historically co-occurring boreal forest conifer species, white spruce (Picea glauca) and balsam fir (Abies …

Aquatic Vegetation Seed Bank Status Of Kentucky Lake, Emily A. Freeman

2023 Midwest Ecology & Evolution Conference

Aquatic vegetation is an important biotic component of reservoir ecosystems. Vegetation reduces erosion by acting as a barrier against current and waves from the lake. Aquatic vegetation also helps to oxygenate the water, stabilize nutrients like carbon, nitrogen, and phosphorus, and provides structure for aquatic vertebrates. The absence of vegetation in Kentucky Lake has been influenced by a fluctuating water table (due to winter lake drawdowns), invasive carp, and the use of herbicides for grass management. The objective of this study is to determine the state of the remaining seedbank within nautical mile markers 45 and 52. By using long-term …

Polyploidy’S Impact On Plant-Herbivore Dynamics: Evidence From Experiments With Water-Lily Aphids And Greater Duckweed, Hannah R. Assour

2023 Midwest Ecology & Evolution Conference

Polyploidy, or whole-genome duplication, is a widespread mutational process in plants with a myriad of phenotypic and genotypic consequences. In addition to consequences incurred by the plant, polyploidy can potentially cascade to alter species interactions. Yet, knowledge of whether ploidy-induced variation has effects on plant-herbivore dynamics is scarce. Here, we test whether aphid herbivores exhibit preference for a diploid or polyploid host cytotype and whether ploidy impacts plant resistance and tolerance to aphids, measured as aphid and plant performance respectively. To answer these questions, we evaluated aphid choice and compared the effects of the water-lily aphid (Rhopalosiphum nymphaeae) …

How Do Vernal Pond Temperature Fluctuations Impact Fitness Through Development?, Shayna Rosenbloom, Alycia Lackey

2023 Midwest Ecology & Evolution Conference

Anthropogenic climate change is driving shifts in natural temperature regimes. In addition to global warming, temperatures are becoming increasingly variable. Many organisms rely on seasonal temperatures across life stages as they grow and reproduce, among other critical aspects of their lives. A lot of work has been done on how increases in mean temperature might impact organisms, but there is a gap in our understanding on the impacts of increases in variation. Amphibians are especially susceptible to environmental change, particularly in earlier life stages. Increases in temperature have been shown to cause shifts in their phenology, decreases in body size, …

Evolution Of A Genus Of Gall Wasp Kleptoparasites, Guerin E. Brown

2023 Midwest Ecology & Evolution Conference

Kleptoparasites do not directly parasitize their hosts but instead steal food and resources, reducing host fitness. Like direct parasites, kleptoparasites can be highly dependent on their hosts such that their evolutionary histories may be linked. Here, we study the evolution of a kleptoparasitic wasp genus, Synergus (Hymenoptera: Cynipidae: Synergini), in relation to their hosts, oak gall wasps (Hymenoptera: Cynipidae: Cynipini). Ovipositing oak gall wasps induce the formation of galls on oaks (Fagaceae) that provide food and shelter to one or more developing gall wasps. Galls induced by different gall wasp species are diverse in size, shape, color, and location …

Where The Wild Mushrooms Are: Species And Secondary Metabolite Diversity In Fungal Species On The Saint Mary’S College Campus, Lex K. Gilman

2023 Midwest Ecology & Evolution Conference

Secondary metabolites in fungi have three main ecological functions: protection against predation, communication with other mushrooms, and assistance in pathogenic interactions. These compounds have also led to the common use of mushrooms as food and in traditional Chinese or other medicinal applications. However, there are currently few good resources for identifying mushroom species in North-Central Indiana. In addition, limited research exists on secondary metabolites in mushrooms. The objective of this research was to create a preliminary database of mushroom species at Saint Mary’s College and conduct preliminary research on the secondary metabolites of these local species. Fungal specimens were identified …

Dairy Bioactives And Functional Ingredients With Skin Health Benefits, Aleksandra Augustyniak, Davide Gottardi, Barbara Giordani, James Gaffey, Helena Mcmahon

Publications

The potential of whey from Italian cheese productions to support skin health and anti-ageing mechanism was studied. The effect of whey on dermal and epidermal cells was evaluated. Whey inhibited the activity of elastase and tyrosinase enzymes by 60% and 32%, respectively. Whey cytotoxicity against tested skin cell lines, human fibroblasts (HDFa) and keratinocytes (HaCaT) was not observed. Moreover, the antioxidant activity of the samples was noted, after treatment with whey intracellular ROS level was decreased by 87% in comparison with the hydrogen peroxide-treated cells. Fibroblasts produced a significant amount of extracellular matrix molecules, collagen I, elastin and glycosaminoglycans as …

Postnatal Developmental Trends In Membrane Excitability And Bk Channel Function In The Rodent Hippocampus, Michael S. Hunsberger

Dissertations (1934 -)

This dissertation outlines the postnatal development of excitability as well as expression and function of BK potassium channels in hippocampal neurons. I used patch clamp electrophysiology to measure how neuronal action potential waveforms and action potential firing frequencies change in early development, and how pharmacological blockade of BK channels affects these properties in hippocampal neurons. I also describe how the protein expression of the BK channel pore-forming α subunit and mRNA expression of different variants of the pore forming α subunit and auxiliary beta-4 subunit changes with development. I demonstrate in both cultured rat hippocampal neurons across the first seven …

Socio-Ecological Interactions Promote Outbreaks Of A Harmful Invasive Plant In An Urban Landscape, T. Trevor Caughlin, Matthew Clark, Louis W. Jochems, Nick Kolarik, Andrii Zaiats, Cody Hall, Jason M. Winiarski, Breanna F. Powers, Martha M. Brabec, Kelly Hopping

Biology Faculty Publications and Presentations

1. Urban landscapes often harbour organisms that harm people and threaten native biodiversity. These landscapes are characterized by differences in socioeconomic context, habitat suitability and patch connectedness. Identifying which spatial differences enable outbreaks of pests, pathogens and invasive species will improve targeted control efforts.

2. We tested hypotheses to explain the distribution and demography of puncturevine Tribulus terrestris, a human-dispersed invasive plant in Boise, a city in the western United States. We hypothesized an increase in puncturevine infestations near low-valued properties with a high proportion of bare ground, the species' preferred microhabitat, that are well connected on the urban road network. …

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Memory Acquisition By Brainless Juvenile Terrestrial Planarians (Bipalium Kewense), Christina Chueiri, Christine Gildea

Transformations: Presentation Slides

Learning is how organisms build and store memories in their brains to aid them in survival. This process occurs as organisms experience new stimuli. Bipalium kewense, a terrestrial planarian, has a very minimal central nervous system. The nervous system of planarians consists of only two nerve cords and two ganglion which serve as a “brain.” This species reproduces by segmenting a small piece of their posterior end as a fragment, which initially lacks most adult structures. The fragments must regenerate the head, brain, mouth, functioning digestive system, and reproductive organs. To determine where memory is stored in these terrestrial planarians, …

Local Translation And Focal Adhesions Are Dysregulated In Down Syndrome, Ashlyn Gotberg, Nikita Kirkise M.S., Kristy Welshhans Ph.D.

Senior Theses

During cellular migration, fibroblasts sense extracellular cues through membrane complexes at their leading edge and can respond to these cues via local translation. Local translation is the process in which mRNAs are transported to subcellular regions and translated in response to specific cues. There is evidence that local translation may be dysregulated in the neurodevelopmental disorder, Down syndrome, which is caused by the triplication of chromosome 21 and results in genome-wide dysregulation of protein expression. To determine if dysregulated local translation contributes to Down syndrome, we used three sets of primary fibroblasts from individuals with Down syndrome and apparently healthy …

Using Podcasts To Bring National Estuarine Research Reserves Into The Classroom For Grades 6-12, Kaitlyn M. Dirr

Senior Theses

In a typical classroom setting, there are significant challenges to exposing students to concepts related to earth sciences and the environment. These challenges are exacerbated when conveying lessons about geographic areas with limited access, such as oceans and coastlines (Louv, 2010). It is now more important than ever for environmental education to improve and adapt to our changing world. Educators may have the opportunity to bring these subjects to life by using media content such as podcasts to introduce students to new places and the scientists, managers and educators that work in those spaces. In the United States, there exists …

Coordination Of Hydraulic And Morphological Traits Across Dominant Grasses In Eastern Australia, Robert J. Griffin-Nolan, Jeff Chieppa, Alan K. Knapp

Biology

1. Leaf hydraulic traits characterize plant drought tolerance and responses to climate change. Yet, plant hydraulics are biased towards northern hemisphere woody species. We collected rhizomes of several perennial grass species along a precipitation gradient in eastern Australia and grew them in an experimental pot study to investigate potential trade-offs between drought tolerance and plant morphology.
2. We measured the following leaf hydraulic traits: the leaf water potential (Ψleaf) at 50% and 88% loss of leaf hydraulic conductance (P50Kleaf and P88Kleaf), the Ψleaf at 50% loss of stomatal conductance (P50gs), leaf turgor loss point (TLP), leaf dry matter content (LDMC), leaf modulus …

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller

Undergraduate Honors Thesis Projects

Lemna minor L., duckweed, is a common aquatic plant used for the phytoremediation of wastewater. Heavy metal contamination poses a significant issue, and numerous studies have been conducted on the efficiency of L. minor’s hyperaccumulation ability of these metals. Calcium oxalate crystals are an important aspect of the growth and development of L. minor, but how they are influenced by the uptake of heavy metal has not been extensively studied. This study aims to determine the effects that lead, cadmium, and copper have on the formation of calcium oxalate crystals, vegetative growth, and chlorophyll a and b content on L. …

Measuring Claim-Evidence-Reasoning Using Scenario-Based Assessments Grounded In Real-World Issues, William L. Romine, Amy Lannin, Ankita Agarwal, Maha Kareem, Emily Burwell

Biological Sciences Faculty Publications

Improving students’ use of argumentation is front and center in the increasing emphasis on scientific practice in K-12 Science and STEM programs. We explore the construct validity of scenario-based assessments of claim-evidence-reasoning (CER) and the structure of the CER construct with respect to a learning progression framework. We also seek to understand how middle school students progress. Establishing the purpose of an argument is a competency that a majority of middle school students meet, whereas quantitative reasoning is the most difficult, and the Rasch model indicates that the competencies form a unidimensional hierarchy of skills. We also find no evidence …

Positive And Relaxed Selective Pressures Have Both Strongly Influenced The Evolution Of Cryonotothenioid Fishes During Their Radiation In The Freezing Southern Ocean, Kevin T. Bilyk, Xuan Zhuang, Chiara Papetti

Department of Biology Faculty Scholarship and Creative Works

Evolution in the chronic cold of the Southern Ocean has had a profound influence on the physiology of cryonotothenioid fishes. However, the suite of genetic changes underlying the physiological gains and losses in these fishes is still poorly surveyed. By identifying the genomic signatures of selection, this study aims to identify the functional classes of genes that have been changed following two major physiological transitions: the onset of freezing temperatures and the loss of hemoproteins. Looking at the changes that followed the onset of freezing temperatures, positive selective pressure was found among a set of broadly acting gene regulatory factors, …

Effects Of Actin-Destabilizing Drugs On Motor Function In Drosophila Melanogaster, Caden Sullivan

Honors Theses

Actin dynamics influence many cellular processes including neural connectivity and function. One way to better understand and study the regulation of these dynamics is to manipulate actin dynamics and observe the effects in motor function as a proxy to changes on the neuromuscular junctions in developing neural tissue. On that line, this investigation hypothesized that changing the actin dynamics during development via drug manipulation results in observable effects in locomotion. One of the common actin regulatory drugs used throughout the literature includes Latrunculin A, which was used in this study. Methods included applying this actin destabilizing drug on developing wildtype …

Could Biological Soil Crusts Act As Natural Fire Fuel Breaks In The Sagebrush Steppe?, Lea A. Condon, Douglas J. Shinneman, Roger Rosentreter, Peter S. Coates

Biology Faculty Publications and Presentations

For decades, large portions of the semi-arid sagebrush ecosystem have been experiencing increased frequency and extent of wildfire, even though small, infrequent fire is a natural disturbance in this ecosystem (Baker, 2006). Increased wildfire is threatening the existence of sagebrush ecosystems and the wildlife species that depend upon them (Baker, 2006; Coates et al., 2016). Increased wildfire in sagebrush ecosystems is often driven by invasive annual grasses, especially cheatgrass, Bromus tectorum (L.). Invasion can initiate a trajectory toward a “grass-fire cycle”, in which cheatgrass increases fine fuel loadings that promote fire, and native plant species do not recover quickly after …

In Vitro Characterization Of Fluorogenic Chemical Tools To Study Human Carboxylesterases (Cess), Taylor Parish Spidle

Undergraduate Honors Theses

Human carboxylesterases (CESs) are enzymes that are responsible for the metabolism of many important pharmaceuticals. Although CESs are key players in the hydrolysis of many ester-containing drugs, they remain understudied. Our group hypothesizes that this is primarily because there are few methods capable of reporting activity in live cells. Here, I report a new series of fluorogenic chemical tools to study the CES activity of one of the two major CESs in humans, CES1, in live cells. MCP-Me, MCP-Et, and MCP-iPr utilize the same carbonate group of a previously developed chemical tool in our group, FCP-1, and work to mimic …

Inference And Reconstruction Of The Heimdallarchaeial Ancestry Of Eukaryotes, Laura Eme, Daniel Tamarit, Eva F. Caceres, Courtney W. Stairs, Valerie De Anda, Max E. Schön, Kiley W. Seitz, Nina Dombrowski, Anna-Louise Reysenbach, Multiple Additional Authors

Biology Faculty Publications and Presentations

In the ongoing debates about eukaryogenesis—the series of evolutionary events leading to the emergence of the eukaryotic cell from prokaryotic ancestors— members of the Asgard archaea play a key part as the closest archaeal relatives of eukaryotes1. However, the nature and phylogenetic identity of the last common ancestor of Asgard archaea and eukaryotes remain unresolved2–4. Here we analyse distinct phylogenetic marker datasets of an expanded genomic sampling of Asgard archaea and evaluate competing evolutionary scenarios using state-of-the-art phylogenomic approaches. We find that eukaryotes are placed, with high confidence, as a well-nested clade within Asgard archaea and as a sister lineage …

Diet Of A Threatened Rattlesnake (Eastern Massasauga) Revealed By Dna Metabarcoding, Alyssa Swinehart, Charlyn Partridge, Amy L. Russell, Arin Thacker, Jennifer Kovach, Jennifer A. Moore

Open Access Publishing Support Funded Articles

Characterizing the diet of imperiled species using minimally invasive methods is crucial to understanding their ecology and conservation requirements. Here, we apply a DNA metabarcoding approach to study the diet of the eastern massasauga rattlesnake (Sistrurus catenatus), a Federally Threatened snake found throughout the Great Lakes region. Eighty-three fecal samples collected across 10 different massasauga populations located in Michigan, USA, were sequenced, with 70 samples containing prey DNA. We used universal metazoan primers and developed a host-specific oligonucleotide blocker to characterize their diet. We identified at least 12 different prey species, with eastern massasaugas exhibiting opportunistic feeding and …