Digital Commons Network^™

Population Physiology, Demography, And Genetics Of Side-Blotched Lizards (Uta Stansburiana) Residing In Urban And Natural Environments, Spencer B. Hudson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildlife populations across the globe are poised to lose their natural habitat to urbanization, yet there is limited information on how different species handle living in cities. Animals in urban environments are often susceptible to novel stressors, which can threaten their individual health and population viability. The physiological characteristics of animals, such as those related to metabolic hormones, oxidative stress, and immunity, are expected to be important for survival in this context. If so, animals persisting in urban areas may demonstrate physiological differences from their natural counterparts, perhaps due to evolutionary change. These potential outcomes have been documented in birds …

A Novel Approach To The Nest Site Characterization Of Two Species Of Critically Endangered Vulture In Gorongosa National Park, Mozambique, Rebecca Colleen Bishop

Boise State University Theses and Dissertations

Vulture populations in Africa face extinction as the result of numerous human-generated threats. Existing geographical and ecological knowledge gaps urgently need to be filled to support targeted conservation action across the vast continent, and ideally halt further decline. My study focuses on two Critically Endangered African vulture species - the White-backed Vulture (Gyps africanus) and the especially rare and poorly understood White-headed Vulture (Trigonoceps occipitalis) - in one of the most understudied countries, Mozambique, and from within a protected area, Gorongosa National Park. Formal documentation of breeding densities, nest site characteristics, and timing is limited for …

Remote Robotic Surgery And Virtual Education Platforms: How Advanced Surgical Technologies Can Increase Access To Surgical Care In Resource-Limited Settings, Youmna A Sherif, Mohammed A Adam, Aimee Imana, Sarnai Erdene, Rachel W Davis

Faculty, Staff and Students Publications

Advanced surgical technologies consist of remote and virtual platforms that facilitate surgical care and education. It also includes the infrastructure necessary to utilize these platforms (e.g., internet access, robotic systems, and simulators). Given that 5 billion people lack access to safe and timely surgical care, the appeal of these technologies to the field of global surgery lies primarily in its ability to eliminate geographical barriers and address surgeon shortages. This article discusses the use of virtual and remote technologies in resource-limited settings, the potential applications of these technologies, the possible barriers to their integration, and the impact these technologies may …

Noncanonical Binding Of Transcription Factors: Time To Revisit Specificity?, Md Abul Hassan Samee

Faculty, Staff and Students Publications

Transcription factors (TFs) are one of the most studied classes of DNA-binding proteins that have a direct functional impact on gene transcription and thus, on human physiology and disease. The mechanisms that TFs use for recognizing target DNA binding sites have been studied for nearly five decades, yet they remain poorly understood. It is classically assumed that a TF recognizes a specific sequence pattern, or motif, as its binding sites. However, recent studies are consistently finding examples of noncanonical binding, that is, TFs binding at sites that do not resemble their sequence motifs. Here we review the current literature on …

Kinase Inhibitor Pulldown Assay Identifies A Chemotherapy Response Signature In Triple-Negative Breast Cancer Based On Purine-Binding Proteins, Junkai Wang, Alexander B Saltzman, Eric J Jaehnig, Jonathan T Lei, Anna Malovannaya, Matthew V Holt, Meggie N Young, Mothaffar F Rimawi, Foluso O Ademuyiwa, Meenakshi Anurag, Beom-Jun Kim, Matthew J Ellis

Faculty, Staff and Students Publications

Triple-negative breast cancer (TNBC) constitutes 10%–15% of all breast tumors. The current standard of care is multiagent chemotherapy, which is effective in only a subset of patients. The original objective of this study was to deploy a mass spectrometry (MS)-based kinase inhibitor pulldown assay (KIPA) to identify kinases elevated in non-pCR (pathologic complete response) cases for therapeutic targeting. Frozen optimal cutting temperature compound–embedded core needle biopsies were obtained from 43 patients with TNBC before docetaxel- and carboplatin-based neoadjuvant chemotherapy. KIPA was applied to the native tumor lysates that were extracted from samples with high tumor content. Seven percent of all …

Exploring Biotic And Abiotic Constraints On Competition For Signal Space Within Birdsong Communities, Joanna M. Sblendorio

Dissertations

With the acceleration of climate change and the conversion of natural ecosystems to human-dominated landscapes, there is concern for the persistence of biodiversity amid novel biotic and abiotic factors. An important outcome of shifting species distributions and environmental disturbance is the formation of novel assemblages. For animals that vocalize for mate attraction and resource defense, the introduction of new species into communities may alter availability of acoustic space for signaling and affect detection of signals by conspecifics. Thus, to minimize signal overlap, species should partition signal space and thereby inhabit well-defined acoustic niches. Yet mixed evidence exists regarding signal space …

The Influence Of Climate And Landscape On Allocapnia Mohri Distribution In Arkansas, Brianna Leigh Annaratone

Graduate Theses and Dissertations

Stoneflies are a globally threatened aquatic insect order. In Arkansas, a diverse group of winter stonefly (Capniidae: Allocapnia) have not been surveyed since the 1980s, likely because species-level identification requires the rarely-collected adult form. Allocapnia mohri, a regional endemic, was previously commonly found in mountainous, intermittent streams from the Ouachita Mountains ecoregion north to the Ozark Highlands, but no species distributional models including land use or climate variables exist to our knowledge. We collected adults from 70 stream reaches from the historic Arkansas range from November to April 2020 and 2021. We modeled distributions using random forest (RF) models populated …

Shedding In The Timber Rattlesnake: Natural Patterns, Endocrinological Underpinnings, Temporal And Energetic Effort, And Integration As A Reptilian Life History Trait, Maxwell D. Carnes-Mason

Graduate Theses and Dissertations

The semi-frequent replacement of the epidermis (ecdysis) is a characteristic trait of reptiles. Whereas all reptiles regularly engage in some degree of skin shedding, skin morphology in snakes necessitates the synchronous replacement of the entire epidermis and facilitates the subsequent removal of the old layer as a single sheet. To date, the ubiquitous process has garnered little attention from researchers because snakes shed with unpredictable timing and frequency and are exceedingly cryptic during ecdytic cycles; previously impeding detailed physiological or ecological investigations of the process in the clade. Because of the lack of study, ecdysis is often viewed as a …

Modeling The Egyptian Goose (Alopochen Aegyptiaca) Invasion; And Future Concerns, Percival Matzinger Marshall

Graduate Theses and Dissertations

In an increasingly interconnected world, the ecological and financial cost of invasive species is expected to continue to climb through the movement of exotic biota. Understanding the driving forces behind how a species invades, what environments promote their establishment, and what impacts they are likely to have on the invaded environment are all critical for management. Waterfowl, order Anseriformes, are one such category of invasive species of concern due to their popularity of accidental introduction, ease of movement, and propensity to affect both terrestrial and aquatic ecosystems. The Egyptian Goose (Alopochen aegyptiaca) is a native to the African continent that …

The Importance Of Soil Carbon In Large-Scale Shrub Removal Practices In The Chihuahuan Desert, Kathleen Schaeffer

Open Access Theses & Dissertations

Shrub encroachment is a worldwide phenomenon that affects multiple biomes at different ecological and anthropogenic levels. In dryland ecosystems, shrub encroachment is a noted concern amongst land managers, as it can lead to a loss of soil resources and biodiversity. To mitigate the negative effects of shrub encroachment in Drylands, land managers can implement large-scale shrub removal practices. These land restoration practices can shift landscapes to novel ecosystems, where resulting plant communities can vary following treatments. Grass recovery or increasing herbaceous cover is often a primary goal of these treatments, however predicting which sites may show higher grass responses to …

Exploring Undergraduate Research Experiences And Perceptions Of Responsible And Ethical Conduct Of Research (Recr) Education In The Biological Sciences And (Bio)Chemistry Disciplines, Bernice Justina Caad

Open Access Theses & Dissertations

Increasing calls to reform undergraduate (UG) education within science, technology, engineering, and mathematics (STEM) disciplines have emphasized the importance of early exposure to responsible and ethical conduct of research (RECR) education. Historically, RECR has primarily been presented at the post-baccalaureate level, leaving many students without a foundational understanding of what responsible research ethics entails during the course of their undergraduate experience. Over the last decade, course-based undergraduate research experiences (CUREs) have emerged as a promising platform to establish expectations of responsible and ethical conduct through greater accessibility and inclusivity, starting at the freshman level. Interestingly, few studies have examined how …

Immunoreactivity Of The Serotonin-Like And Fmrf-Amide-Like Nervous Systems Of Four Gnesiotrochan Rotifers, Robert Neil Walsmith

Open Access Theses & Dissertations

Reorganization of the nervous system during metamorphosis is a common phenomenon across taxa (species through phyla). In phylum Rotifera, sessile species belonging to the superorder Gnesiotrocha metamorphose from free-swimming larvae into sessile adults. Once mature, gnesiotrochan rotifers display an intricate corona or infundibulum associated with lifestyle modalities and feeding behaviors. Here, I examine serotonin-like immunoreactivity (SLIR) and FMRF-amide-like immunoreactivity (FLIR) of the gnesiotrochans Lacinularia flosculosa, Collotheca ferox, and Acyclus inquietus with a focus on life stage and sex. Based on previous research, I hypothesize that 1) innervation of the larval nervous system will vary according to the lifestyle constraints observed …

Best Practices To Promote Field Science Safety, Kristen Yarincik, Anne Kelly, Terry Mcglynn, Robin M. Verble

Biological Sciences Faculty Research & Creative Works

Interventions are necessary to address the ongoing epidemic of sexual harassment and assault in field settings. An evidence-based approach to identifying specific interventions will be most effective at promoting the safety of scientists. We present the results of a workshop conducted by experts in field biology and the study of sexual harassment and assault that identified a comprehensive set of best practices for individuals and organizations. These recommendations are grounded in peer-reviewed scholarship and are separated into four topics: culture change, accountability, policy development, and reporting. The resulting report of the workshop recommends 44 practices, categorized by the resources required …

Suitability And Nutritional Analysis Of Spotted Lanternfly Habitats, Kathryn Hickey

Biology Student Scholarship

The spotted lanternfly is an invasive bug that has recently started taking over the Northeastern US, and has even made it to Rhode Island. Although this species is intriguing with its bright red back and spots, it is hurting native plant species and the agriculture industry by feeding on crops, like grapes and cherries. It uses a tongue-like mouthpart to pierce through the plant and feed directly on the sap inside. This punctures plant stems and causes them to drip sap, wither, and even mold. The lanternfly’s most preferred host plant is the invasive tree-of-heaven, which grows and spreads quickly …

Effect Of Decreased Snowfall During An Atypical Winter On A Rare Butterfly’S Host Plant, Breelyn Gilbert

Biology Student Scholarship

In the Northeastern US, one result of climate change is decreased snowfall and earlier snowmelt. Such changes can alter the relationship between climate and the timing of cyclical biological phenomena— the phenology of organisms. This is especially concerning for species that interact with one another such as butterflies and their host plants. For this study, I focused on the host plant of the rare frosted elfin butterfly. The frosted elfin is a species of concern in RI, MA, and CT, among 8 other states, and they are a host plant specialist only laying eggs on yellow wild indigo (Baptisia …

The Third Domain Of T Cells In The Evolution Of Novel Immune Cell Types, Kimberly Anne Morrissey

Biology ETDs

αβ and γδ T cells play an essential role in the adaptive immune response and are present in the majority of jawed vertebrates. The T cell receptor (TCR) structure and genetic organization have been generally stable throughout 400 million years of gnathostome evolution. However, the TCRδ chain has displayed degrees of plasticity unseen in other TCR isoforms. One such form appears to be a chimera of a TCR chain with an antibody-like binding domain, known as VHδ and has been found a wide array of vertebrates including cartilaginous fish, coelacanths, amphibians, tuataras, birds, crocodilians, and monotreme mammals. Another, more extreme …

An Ecological Survey Of East Texas Salamanders Across The Camp Tyler Outdoor Field School In Smith County, Texas, Justin C. Hunt

Biology Theses

Amphibians are a unique class of organisms with a very long and storied evolutionary history of survival. Many modern amphibian clades occupy several vital ecological roles within their native freshwater environments. One of these roles, typically includes functioning as an ecological indicator species, whereby the presence of stable and diverse populations of many amphibian species, including salamanders, within a freshwater ecosystem have long been considered ecological indicators of good habitat quality and stable ecosystem health. Similarly, salamanders also function as important members of their local food webs and act as valuable mediators of complex trophic hierarchies to facilitate nutrient cycling …

Uvrd1 Helicase Of Mycobacterium Tuberculosis That Can Be Activated By Multiple Unique Mechanisms, Ankita Chadda

Arts & Sciences Graduate Student Theses and Dissertations

Abstract Mycobacterium tuberculosis (Mtb) causes tuberculosis disease in humans and is one of the leading causes of death worldwide due to infectious agents. During infection, Mtb is exposed to reactive oxygen species and reactive nitrogen intermediates from the host immune response that causes DNA damage. UvrD-like helicases are involved in DNA repair and use energy from ATP hydrolysis to translocate on single stranded DNA (ssDNA) or unwind double-stranded DNA (dsDNA) to remove the damaged DNA strand. Previous studies on UvrD-like helicases have shown that they exist in a monomer-dimer equilibrium and unwind only as dimers in the absence of accessory …

Monitoring The Effects Of Poultry Waste On Fishes And Macroinvertebrates In The Sabine River, Karley R. Parker

Biology Theses

Freshwater is a vital resource that provides life and sustainability for almost all organisms on Earth. It is important to maintain its health and protect it from emerging pollutants that pose a threat to the organisms that use it. Pollution continues to threaten the well-being of the environment’s freshwater sources all around the world that could lead to damaging effects in the future. The Sabine River is a major freshwater resource in the east Texas and western Louisiana areas that provides a habitat for thousands of organisms as well as other domestic uses for humans. In 2019, a waste discharge …

The Effect Of Integrated Pest Management On Honey Bee Behavior And Colony Level Health, Kaitlyn Bresnahan

Biology Student Scholarship

Honey bees are the world’s most commercially valuable pollinators, and they face many threats. The greatest challenge facing honey bees is Varroa mites, which are parasitic mites that feed on bees’ fat bodies, killing bees and spreading diseases. While chemical treatments are available to stop the spread of mites, these treatments are insecticides, which are not ideal to put in a hive of insects. A popular organic method of mite management is using drone comb. Drone brood is preferentially chosen by mites for ovipositing and development because of its larger size and longer development time, so removing drone brood can …

Diversity Of Bees In Trees And Their Foraging Preferences On An Urban College Campus, Aidan Castricone

Biology Student Scholarship

Pollinators collect nutrients from blooming flowers; pollen provides proteins and fats, nectar provides carbohydrates. The few plants that bloom during early Spring are trees such as crabapple (Malus sp). Current research however, mainly focuses on pollinators that forage on the ground and overlooks pollinators foraging in the canopy of trees. Past research showed increased generalization of pollen foraging in bees as seasons move from spring to summer. Here I identified which bee species forage in the canopy on Providence College campus and will analyze the pollen collected using microscopy. This data can inform more specific research on diet breadth, …

Novel Collective Prey Evasion Response Of Pseudomonas Aeruginosa Pao1 To Soil Micropredator Myxococcus Xanthus Dk1622, Joshua Lummus

Master of Science in Integrative Biology Theses

The search for alternative therapeutics is on the rise because of the threat of multi-drug resistant bacteria. Looking at the hunting strategy of soil-based bacterial micropredators is one avenue of exploration. Myxobacteria are soil predators with a wide prey range including Gram-negative and Gram-positive bacteria, fungi, and archaea. In our lab we discovered a distinct and novel anti-predation behavior in Pseudomonas aeruginosa strain 01. P. aeruginosa is an opportunistic and nosocomial pathogen responsible for 10% of all hospital acquired infections. P. aeruginosa is also an ESKAPE pathogen, which are leading causes of nosocomial infections throughout the world, and they are …

Microbial Commensals Of Tricolored Bats In Georgia And Their Probiotic Potential, Jordyn Upton

Master of Science in Integrative Biology Theses

Pseudogymnoascus destructans (Pd) is a fungal pathogen that has been spreading in North American bats since its initial discovery in 2006. P. destructans is the causative agent of white-nose syndrome (WNS), a disease leading to as much as a 90% decrease in some eastern United States bat populations, with select hibernacula experiencing complete extirpation of the hibernating population. To date, there is no effective scaled probiotic for WNS, but probiotics inoculums have shown success in the management of other recently emerged fungal pathogens of wildlife, such as chytridiomycosis in amphibians. Host microbiomes have come under investigation in other disease systems …

Loss Of Flockmates Weakens Winter Site Fidelity In Golden-Crowned Sparrows (Zonotrichia Atricapilla), Anastasia E. Madsen, Bruce E. Lyon, Alexis S. Chaine, Theadora A. Block, Daizaburo Shizuka

School of Biological Sciences: Faculty Publications

Animal social interactions have an intrinsic spatial basis as many of these interactions occur in spatial proximity. This presents a dilemma when determining causality: Do individuals interact socially because they happen to share space, or do they share space because they are socially linked? We present a method that uses demographic turnover events as a natural experiment to investigate the links between social associations and space use in the context of interannual winter site fidelity in a migratory bird. We previously found that golden-crowned sparrows (Zonotrichia atricapilla) show consistent flocking relationships across years, and that familiarity between individuals …

Transcriptional Regulation Of The Synthesis And Secretion Of Farnesol In The Fungus Candida Albicans: Examination Of The Homann Transcription Regulator Knockout Collection, Daniel Gutzmann, Jaxon J. Kramer, Brigid M. Toomey, Cory Honsinger Thomas Boone, Audrey L. Atkin, Kenneth Nickerson

School of Biological Sciences: Faculty Publications

Candida albicans is an efficient colonizer of human gastrointestinal tracts and skin and is an opportunistic pathogen. C. albicans exhibits morphological plasticity, and the ability to switch between yeast and filamentous morphologies is associated with virulence. One regulator of this switch is the quorum sensing molecule farnesol that is produced by C. albicans throughout growth. However, the synthesis, secretion, regulation, and turnover of farnesol are not fully understood. To address this, we used our improved farnesol assay to screen a transcription regulator knockout library for differences in farnesol accumulation in whole cultures, pellets, and supernatants. All screened mutants …

Transcriptional Regulation Of The Synthesis And Secretion Of Farnesol In The Fungus Candida Albicans: Examination Of The Homann Transcription Regulator Knockout Collection, Daniel Gutzmann, Jaxon J. Kramer, Brigid M. Toomey, Cory Honsinger Thomas Boone, Audrey L. Atkin, Kenneth Nickerson

School of Biological Sciences: Faculty Publications

Candida albicans is an efficient colonizer of human gastrointestinal tracts and skin and is an opportunistic pathogen. C. albicans exhibits morphological plasticity, and the ability to switch between yeast and filamentous morphologies is associated with virulence. One regulator of this switch is the quorum sensing molecule farnesol that is produced by C. albicans throughout growth. However, the synthesis, secretion, regulation, and turnover of farnesol are not fully understood. To address this, we used our improved farnesol assay to screen a transcription regulator knockout library for differences in farnesol accumulation in whole cultures, pellets, and supernatants. All screened mutants produced …

Commercial Fishing Identification Guide 2023, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Occasional Publications

Throughout this guide you will see all or some of the marine bioregion symbols with each fish illustration, indicating where the species is most likely to occur.

This guide has been developed to help you identify the more common species in Western Australia you may encounter. The purpose of this guide is to enhance consistent and accurate species identification.

If you are unsure about a particular species (or if it is not in this guide), please discuss it with a representative of the Department of Primary Industries and Regional Development, Western Australia.

Investigating The Costs And Benefits Of A Nutritional Fungal Endosymbiont, Nolan L. Perryman

Biology ETDs

Research on insect symbiosis is dominated by insect–bacteria models, however, the role of fungal symbionts in insects is poorly understood. Fungi are de novo synthesizers of many essential nutrients, therefore, insect–fungal pairings can permit specialization on nutritionally deprived diets. Here, I address the costs and benefits of symbiosis using a system of beetle–fungal symbiosis. Specifically, I ask 1) when reared on a low-quality diet, does the beetle increase the number of viable symbionts to compensate for a heightened nutritional demand, and 2) Is the conferred fitness benefit of the symbiont only present in low-quality diets? When reared on low-quality diets, …

Assessment Of Dna Concentration And Degradation From Extracted Bumble Bee Thorax Muscle 2023, Joel Lado, Ai Wen, Kenneth Elgersma

Summer Undergraduate Research Program (SURP) Symposium

Deoxyribonucleic acid (DNA) is the foundation of an organism's ability to develop, survive, and reproduce. High genomic DNA is imperative for many scientific studies and applications such as Biotechnology, Genetics, & Pharmaceuticals. The separation of genomic DNA once purified from other cellular components (RNA, protein) can be sequenced to study entire genomes, develop antibodies, and limit genetic mutations.

Although a very stable molecule, DNA is susceptible to degradation under normal cellular conditions to various factors. Hence it is essential to properly extract genetic material from bee specimens, to better understand their population genetics.

DNA often needs to be stored for …

Comparison Of Gut And Leg Pollen Composition In Female Halictus Ligatus With Flower Source, Sriya Kalala, Ai Wen

Summer Undergraduate Research Program (SURP) Symposium

It is commonly believed by bee biologists that wild bees visit one single flower species during each trip to make nest provision. However, the exact foraging behavior is not clear and may vary in different species of bees, and therefore the pollination patterns have continued to be studied. When a bee undergoes the process of pollination, the majority of the pollen consumed by the bee passes through the digestive tract and remains undigested. However, when the pollen is digested and used as an energy source, the pollen grain digestion occurs in the midgut intestine within the bee instead of the …