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Dynamics And Inhibition Of Abc And Rnd Transporters Associated With Multidrug Resistances In Cancer And Pathogenic Bacteria, Hang Zhao

Biological Sciences Theses and Dissertations

The overexpression of efflux transporters is deemed to be one of the important mechanisms that contribute to Multidrug Resistance (MDR). In this dissertation, three transporters of significance were studied, the Breast Cancer Resistance Protein (BCRP/ABCG2) in a variety of cancer cells, the mtrCDE transporter from Neisseria gonorrhoeae, and the acrAB-tolC transporter from Escherichia coli. It is well known that the overexpression of BCRP in cancer cells results in these cells being resistant to the effects of many chemotherapeutics. This results in multidrug resistance for this cancer because of the fact that BCRP can transport many different types of …

The Coastal Monitor: Vol. 10 No. 2, John Tanacredi Ph.D.

The Coastal Monitor

Stephen J. Gould’s prophetic piece, “The Golden Rule: A Proper Scale for Our Environmental Crisis”, noted that, “Patience enjoys a long pedigree of favor”, which he elaborated, “usually involves a deep understanding of the fundamental principle… rarely grasped in daily life – the effects of scale.” Scientists observe changes incessantly, in dimensions and time, from microscopic conditions of cellular biology to the inconceivable distances of galaxies and their influences on Earth.

Role Of G1p3-Rab5 Interaction In Breast Cancer Gene Expression, Madelyn Terrell

Honors Theses

Breast cancer is one of the most common cancers worldwide with millions of cases diagnosed each year. Despite modern treatments, over 40% of patients can expect to relapse after initial cancer eradication. Consequently, it is crucial to unravel the mechanisms by which breast cells confer their cancerous properties to develop novel treatments that improve patient survival. G1P3 is a mitochondrial localized protein found to be upregulated 50x in estrogen receptorpositive breast cancer and is correlated with overall poor patient survival. G1P3-overexpressing cells display increased amounts of interferon-stimulated genes (ISGs) and G1P3 as an ISG indicates these genes potential to promote …

Patterns Of Cytonuclear Discordance And Divergence Between Subspecies Of The Scarlet Macaw (Ara Macao) In Central America, Matthew L. Aardema, Kari L. Schmidt, George Amato

Department of Biology Faculty Scholarship and Creative Works

The scarlet macaw, Ara macao, is a neotropical parrot that contains two described subspecies with broadly discrete geographical distributions. One subspecies, A. m. macao, is found from South America north into southwestern Costa Rica, while the second subspecies, A. m. cyanoptera, is found from eastern Costa Rica north into central Mexico. Our previous research using mitochondrial data to examine phylogeographical divergence across the collective range of these two subspecies concluded that they represent distinct evolutionary entities, with minimal contemporary hybridization between them. Here we further examine phylogenetic relationships and patterns of genetic variation between these two subspecies using a dataset …

Loggerhead Shrike Occurrence Along Urban Gradients In South Carolina’S Coastal Plain, Michelle A. Krauser, Christopher E. Hill

Biology

The urban landscape is understudied compared to less developed ecosystems, despite providing suitable habitat for wildlife, including some species of grassland birds. Loggerhead Shrikes (Lanius ludovicianus) are predatory songbirds that frequent grasslands and have been observed using grassy areas in developed and more natural landscapes, yet remain largely unstudied in developed landscapes. We investigated the effects of development, measured as impervious surface percentage, on Loggerhead Shrike occurrence in urban areas in South Carolina, U.S.A, and also tracked canopy cover (%), highway density (m/ha), non-highway road density (m/ha), total road density (m/ha), and powerline density (m/ha). The first author searched 300 …

Brilliant Bird Brains: Object Recognition In Herring Gulls (Larus Argentatus Smithsonianus), Kristen M. Covino, F. Dylan Titmuss, Joseph E. Brosseau, Dora C. Donacik, Maddie E. Ellms, Alyssa R. Nowicki, Daphne Okuyama, Jessica M. Robinson, Katherine A. Schickfus, Eliza L. Stanley, Mary Elizabeth Everett

Biology Faculty Works

The ability to recognize and associate objects with prior experiences has been demonstrated in several avian taxa. Corvids, for example, recognize visual stimuli associated with negative previous experiences and will modify their behavioral responses according to the level of perceived threat. However, the extent to which similar memory-based behaviors exist in seabirds is poorly understood. Our study investigated object recognition by Herring Gulls (Larus argentatus smithsonianus) on their breeding grounds, the site of a long-term monitoring project. When handling gulls and conducting field research, researchers wear standard bicycle helmets as a protective measure against physical aggression by nesting …

Predicting Stringent Quantiferon-Tb Gold Plus Conversions In Contacts Of Tuberculosis Patients, Sheng-Wei Pan, Donald G. Catanzaro, Marva Seifert, Rehan Syed, Naomi Hillery, Mei-Lin Ho, Valeriu Crudu, Elena Tudor, Nelly Ciobanu, Alexandru Codreanu, Antonino Catanzaro, Timonthy C. Rodwell

Biological Sciences Faculty Publications and Presentations

Objectives

Methods

Results

College Of Natural Sciences Newsletter, September - October 2023, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Page 1 Dean's Message
Page 2 New Faculty an Staff for the Fall 2023 Semester
Page 3 Awards
Page 4 Student Ambassadors in CNS
Page 5 Meet our Jacks
Page 6-8 Events
Page 9-11 Media Coverage of CNS
Page 12-13 Spring 2023 Dean's List
Page 14 Open PRAIRIE Data

Farnesol Brain Transcriptomics In Cns Inflammatory Demyelination, William J. Doyle, Dana Walters, Xutong Shi, Kristina Hoffman, Krisztian Magori, Jean-Baptiste Roullet, Javier Ochoa-Repáraz

Biology Faculty Publications and Presentations

Background: Farnesol (FOL) prevents the onset of experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS).

Objective: We examined the transcriptomic profile of the brains of EAE mice treated with daily oral FOL using next-generation sequencing (RNA-seq).

Methods: Transcriptomics from whole brains of treated and untreated EAE mice at the peak of EAE was performed.

Results: EAE-induced mice, compared to naïve, healthy mice, overall showed increased expression in pathways for immune response, as well as an increased cytokine signaling pathway, with downregulation of cellular stress proteins. FOL downregulates pro-inflammatory pathways and attenuates the immune response in EAE. FOL …

Compensating For Effects Of Autotomy On Locomotion In Stink Bugs (Halyomorpha Halys), Lilian C. Jubb

Longwood Senior Thesis Proposal

Autotomy is the voluntary release of an appendage, typically to avoid predation or entrapment. Autonomy of a single leg can have minimal effects on locomotion in arthropods with more than six legs. Hexapods, however, use a double-tripod gait which is completely disrupted with the loss of a single leg, thus locomotor performance should be greatly reduced leading to lower survival. Autotomy is so costly to locomotion that some insects regenerate a lost limb in a subsequent molt leading to reduced investment in other important functions (e.g. reproduction). Autotomy persists in adult hexapods with no opportunity for regeneration, which suggests some …

Moderate Heat Stress-Induced Sterility Is Due To Motility Defects And Reduced Mating Drive In Caenorhabditis Elegans Males, Nicholas B. Sepulveda, Donald Chen, Lisa N. Petrella

Biological Sciences Faculty Research and Publications

Moderate heat stress negatively impacts fertility in sexually reproducing organisms at sublethal temperatures. These moderate heat stress effects are typically more pronounced in males. In some species, sperm production, quality and motility are the primary cause of male infertility during moderate heat stress. However, this is not the case in the model nematode Caenorhabditis elegans, where changes in mating behavior are the primary cause of fertility loss. We report that heat-stressed C. elegans males are more motivated to locate and remain on food and less motivated to leave food to find and mate with hermaphrodites than their unstressed counterparts. …

Transcriptomics Of Temporal- Versus Substrate-Specific Wood Decay In The Brown-Rot Fungus Fibroporia Radiculosa, Byoungnam Min, Steven Ahrendt, Anna Lipzen, Cristina E. Toapanta, Robert A. Blanchette, Dan Cullen, David Hibbett

Biology

Brown-rot fungi lack many enzymes associated with complete wood degradation, such as lignin-attacking peroxidases, and have developed alternative mechanisms for rapid wood breakdown. To identify the effects of culture conditions and wood substrates on gene expression, we grew Fibroporia radiculosa in submerged cultures containing Wiley milled wood (5 days) and solid wood wafers (30 days), using aspen, pine, and spruce as a substrate.

Genome Assembly Of The Foot-Flagging Frog, Staurois Parvus: A Resource For Understanding Mechanisms Of Behavior, Mika A. Holtz, Riccardo Racicot, Doris Preininger, Adam M.M. Stuckert, Lisa A. Mangiamele

Biological Sciences: Faculty Publications

Elaborate and skilled movements of the body have been selected in a variety of species as courtship and rivalry signals. One roadblock in studying these behaviors has been a lack of resources for understanding how they evolved at the genetic level. The Bornean rock frog (Staurois parvus) is an ideal species in which to address this issue. Males wave their hindlimbs in a "foot-flagging"display when competing for mates. The evolution of foot flagging in S. parvus and other species is accompanied by increases in the expression of the androgen receptor gene within its neuromuscular system, but it remains unclear what …

Pollinators Of Gonzaga University, Sophie O'Shei, Gary Chang

Biology Student Scholarship

This data is from a public science project conducted by Sophie O'Shei (Gonzaga '24) investigating pollinator diversity in and around Gonzaga's campus. Photos of plants and pollinators were submitted using iNaturalist, an online platform and app that allows users to upload photos of animals, plants, insects, etc. and identifies their species using AI. It also tracks where photos were taken. Users were then able to go through uploaded images and suggest what species they identify in the photo, which can confirm or correct the AI's identification.

The project started during Earth Week 2023, giving students, staff, and other members …

Cannabinoid Receptor Type 1 Regulates Sequential Stages Of Migration And Morphogenesis Of Neural Crest Cells And Derivatives In Chicken And Frog Embryos, Amira Mahomed, Daljit Girn, Afrin Pattani, Brian K. Wells, Chloe C. King, Sonya Patel, Harsimran Kaur, Christina M. Noravian, Jessica Sieminski, Chi Pham, Halley Dante, Maxellende Ezin, Tamira Elul

Biology Faculty Works

The main cannabinoid receptor CB1R first shows expression during early neurula stage in chicken (Gallus gallus) embryos, and at early tailbud stage in the frog (Xenopus laevis) embryos. This raises the question of whether CB1R regulates similar or distinct processes during the embryonic development of these two species. Here, we examined whether CB1R influences the migration and morphogenesis of neural crest cells and derivatives in both chicken and frog embryos. Early neurula stage chicken embryos were exposed to arachidonyl-2ʹ-chloroethylamide (ACEA; a CB1R agonist), N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251; a CB1R inverse agonist) or Blebbistatin (nonmuscle Myosin II inhibitor) in ovo and examined during …

Application Of Computational Tools To Identify Variations Associated With Peromyscus Dominant Spot, Zhenhua Shang

Theses and Dissertations

Peromyscus are the most prevalent mammalian species in North America, and they're employed extensively in both laboratory and field studies. P. maniculatus and P. polionotus, the deer mouse and the old-field mouse, are closely related and can produce viable and fertile hybrid offspring. However, a comprehensive database of polymorphisms, particularly in those kept as living stocks and supplied to university researchers, is lacking. In chapter 2, we performed comparative genomic study on P. maniculatus and P. polionotus and used the polymorphic data to identify the range of genetic complexity that underlies physiological and behavioral differences between two species. We also …

The Himalayan Uplift And Evolution Of Aquatic Biodiversity Across Asia: Snowtrout (Cyprininae: Schizothorax) As A Test Case, Binod Regmi, Marlis R. Douglas, Karma Wangchuk, Zachery D. Zbinden, David R. Edds, Singye Tshering, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Global biodiversity hotspots are often remote, tectonically active areas undergoing climatic fluctuations, such as the Himalaya Mountains and neighboring Qinghai-Tibetan Plateau (QTP). They provide biogeographic templates upon which endemic biodiversity can be mapped to infer diversification scenarios. Yet, this process can be somewhat opaque for the Himalaya, given substantial data gaps separating eastern and western regions. To help clarify, we evaluated phylogeographic and phylogenetic hypotheses for a widespread fish (Snowtrout: Cyprininae; Schizothorax) by sequencing 1,140 base pair of mtDNA cytochrome-b (cytb) from Central Himalaya samples (Nepal: N = 53; Bhutan: N = 19), augmented with 68 GenBank …

The Role Of Insulin Signaling In Controlling Oogenesis In Drosophila Melanogaster, Tancia W. Bradshaw

Theses and Dissertations

Adult stem cell activity responds to organismal nutrient status to maintain and support tissue homeostasis. Despite the intricate relationship between dietary input, adipose tissue and peripheral organ function, we are at the tip of the iceberg regarding our understanding of the cellular and molecular mechanisms underlying adipose communication to other tissues. The Drosophila ovary receives nutritional signals from the fat body with multiple nutrient-sensing pathways functioning in adipocytes to control distinct stages of oogenesis. Insulin/insulin-like growth factor signaling (IIS) within adult adipocytes remotely controls oocyte production at distinct stages of oogenesis. The PI3K/Akt1 axis in adipocytes promotes germline stem cell …

Miocene Phytolith And Diatom Dataset From 10.3myo Diatomite Formation, Fernley, Nevada, Usa, Jacopo Niccolò Cerasoni, Megan C. O'Toole, Richa Patel, Yoel E. Stuart

Biology: Faculty Publications and Other Works

Phytoliths are opal silica particles formed within plant tissues. Diatoms are aquatic, single-celled photosynthetic algae with silica skeletons. Phytolith and diatom morphotypes vary depending on local environmental and climatic conditions and because their silicate structures preserve well, the study of phytolith and diatom morphotypes can be used to better understand paleoclimatic and paleoenvironmental dynamics and changes. This article presents original data from an 820cm-deep stratigraphy excavated at the Hazen diatomite deposits, a high-elevation desert paleolake in the Fernley District, Northern Nevada, USA. The site has been studied for an assemblage of fossilized threespine stickleback, Gasterosteus doryssus, that reveal adaptive …

Sex And Stress Modulate Pupal Defense Response In Tobacco Hornworm, Sukhman Singh, Sakshi Watts, Ishveen Kaur, Isabella Rodriguez, Jessica Ayala, Dariela Rodriguez, Elian Martin, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

In insects, larval and adult defenses against predators have been well studied. However, pupal (also known as resting stage) defenses have been overlooked and not examined thoroughly. Although some pupa possess antipredator strategies such as hairs, spines, cryptic coloration, and exudation of chemicals, few studies have tested these responses and the factors affecting them. Here, we investigated the behavioral responses in tobacco hornworm Manduca sexta that pupates in soil by introducing an external stimulus using vibrations from an electric toothbrush to mimic predation. We observed that M. sexta made violent wriggling (twitching), followed by pulsating movements in response to the …

The Effects Of Landscape And Yard Features On Mammal Diversity In Residential Yards Within Northwest Arkansas, Usa, Emily P. Johansson, Brett A. Degregorio

Biological Sciences Faculty Publications and Presentations

The human footprint is rapidly expanding, and wildlife habitat is continuously being converted to human residential properties. Surviving wildlife that reside in developing areas are displaced to nearby undeveloped areas. However, some animals can co-exist with humans and acquire the necessary resources (food, water, shelter) within the human environment. This ability to coexist may be particularly true when development is low intensity, as in residential suburban yards. Yards are individually managed “greenspaces” that can provide a range of food (e.g., bird feeders, compost, gardens), water (bird baths and garden ponds), and shelter (e.g., brush-piles, outbuildings) resources and are surrounded by …

The Effect Of Antibiotics On Immune Cell Infiltration Of Colon In A Mouse Model Of Early Onset Colorectal Cancer, Valerie Hinsch

Theses and Dissertations

Early onset colorectal cancer (EOCRC) is an aggressive form of colorectal cancer diagnosed before the age of 50. While colorectal cancer cases in patients 50 years and older have been declining, EOCRC cases have been steadily increasing globally in the last few decades, and the cause is unknown. Many agents, called exposomes, have been associated with EOCRC based on their emergence and prevalence prior to and in parallel with the rise in EOCRC cases. Among these are antibiotics (Abx). Abx are indispensable in our fight against infectious diseases, however their overuse is becoming a global epidemic. They are prescribed globally, …

Dephosphorylation Of Mpk6 And Mpk3 By Phosphatases Modulates Responses To Herbivory In Arabidopsis, Claire Teresa Hann

Theses and Dissertations

As sessile organisms, plants rely on extracellular signals and chemical responses to defend against stressors. Extracellular stress signals inducing defense responses originate both from biotic or abiotic threats, and from products of tissue damage or hormones. In defense against herbivores, essential responses include rapid generation of jasmonic acid (JA) at the wound site, followed by the global expression of genes that impede digestion and growth. Early defense gene expression amplifies JA production, while later JA-responsive gene expression causes the accumulation of defense products. MAP kinase (MAPK) pathways play a central role in mediating the perception of self, non-self, and hormonal …

Characterizing Allosteric Regulation And Conformational Dynamics In Staphylococcus Aureus Pyruvate Carboxylase, Amanda Joan Laseke

Dissertations (1934 -)

Pyruvate carboxylase (PC) produces oxaloacetate from pyruvate and bicarbonatein an ATP-dependent manner. The catalytic activity of PC places it at a pivotal intersection between catabolism and anabolism. Understanding the essential metabolic role of PC requires a more complete description of how its activity is regulated and how that regulation is manifested through changes in conformational dynamics. PC activity is regulated by the mutually exclusive binding of the allosteric activator, acetyl-CoA, and the allosteric inhibitor, L-aspartate. The binding site for acetyl- CoA has largely been identified, but the binding location for the acetyl moiety is unknown. Given that the acetyl moiety …

Genome-Wide Association Study And Genomic Prediction Of Fusarium Wilt Resistance In Common Bean Core Collection, Kenani Chiwina, Haizheng Xiong, Gehendra Bhattarai, Ryan Dickson, Theresa Makawa Phiri, Yilin Chen, Ibtisam Alatawi, Derek Dean, Neelendra K. Joshi, Yuyan Chen, Awais Riaz, Paul Gepts, Mark Brick, Patrick Byrne, Howard Schwartz, James B. Ogg, Kristen Otto, Amy Fall, Jeremy Gilbert, Ainong Shi

Horticulture Faculty Publications and Presentations

The common bean (Phaseolus vulgaris L.) is a globally cultivated leguminous crop. Fusarium wilt (FW), caused by Fusarium oxysporum f. sp. phaseoli (Fop), is a significant disease leading to substantial yield loss in common beans. Disease-resistant cultivars are recommended to counteract this. The objective of this investigation was to identify single nucleotide polymorphism (SNP) markers associated with FW resistance and to pinpoint potential resistant common bean accessions within a core collection, utilizing a panel of 157 accessions through the Genome-wide association study (GWAS) approach with TASSEL 5 and GAPIT 3. Phenotypes for Fop race 1 and race …

South Coast Offshore Crustacean Resource Of Western Australia Harvest Strategy, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Management Papers

Harvest strategies for aquatic resources in Western Australia (WA) that are managed by the Department of Primary Industries and Regional Development (DPIRD, the Department) are formal documents that ensures decision-making processes are consistent with the principles of Ecologically Sustainable Development (ESD; Fletcher 2002) and Ecosystem Based Fisheries Management (EBFM; Fletcher et al. 2012). The objectives of ESD are reflected in the objects of the Fish Resources Management Act 1994 (FRMA) and the Aquatic Resources Management Act 2016 (ARMA), which is anticipated replace the FRMA once enacted. At this point, the Fish Resources Management Regulations 1995 (FRMR) is also anticipated to …

Exploring The Correlation Between Metacognitive Skills And Cognitive Learning Outcomes Across Various Biology Learning Models In High Schools, Ninik Kristiani, Herawati Susilo, Aloysius Duran Corebim, Fatchur Rohman

Jurnal Pendidikan Sains

In Malang City, a notable observation within the realm of high school biology education suggests a prevalent lack of metacognitive knowledge among teachers, hindering their efficacy in nurturing students' metacognitive capacities. Such deficiency yields implications for diminished cognitive abilities among students. Recognizing this, there emerges a pressing need for an enhanced pedagogical framework capable of fostering metacognitive skills adeptly. One promising approach involves integrating inquiry-based learning and problem- based mindful pedagogy (PBMP). This amalgamation is anticipated to synergistically harness the potential of both methodologies in bolstering metacognitive skills alongside cognitive learning outcomes. This study adopts a correlational research design, wherein …

Analysis And Unraveling Misconceptions: Examining Tenth-Grade High School Students’ Errors In Hots Questions On Exponential Equations And Effective Scaffolding Strategies, Nunik Indayani, Erry Hidayanto, Sisworo Sisworo

Jurnal Pendidikan Sains

The objective of this study is to elucidate the errors made by tenth-grade students at SMA Hati Bilingual Boarding School Probolinggo, Indonesia, when tackling Higher Order Thinking Skills (HOTS) questions on exponential equations, along with the scaffolding process employed to mitigate these errors. Employing qualitative research methods, four participants were chosen based on their performance in completing HOTS questions on exponential equations, followed by in-depth interviews. The identified student errors encompass basic errors, appropriate errors, missing information, and partial insights. The analysis aligns these errors predominantly with Brodie’s error taxonomy. Furthermore, the study highlights the efficacy of instructional scaffolding, utilizing …

Analysis Of Student Engagement And Perceptions Of Community Of Inquiry (Coi)-Based Blended Learning In The Chemical Separation Subject On Chromatography, Agita Dzulhajh Anggraini, Surjani Wonorahardjo, Yudhi Utomo

Jurnal Pendidikan Sains

Learning in higher education has often been conducted solely through face-to-face, teacher- centered approaches. Implementing Community of Inquiry (CoI)-based blended learning in chromatography is crucial as it offers students a novel learning experience. This pre- experimental study, utilizing a one-group pretest-posttest design, aimed to describe students’ perceptions of CoI-based blended learning in chromatography. Data were collected using a questionnaire comprising 34 statements. The results indicated that students had a neutral perception of CoI-based blended learning. While a community of inquiry has been established, it has not yet reached its full potential, as students have not fully experienced social presence in …

The Identification Of Students’ Conceptual Mastery Of Fluid Statics: An Overview Of Gender, Maria Claudia Sodakain, Sentot Kusairi, Nandang Mufti, Jane Koswojo

Jurnal Pendidikan Sains

This study aimed to assess the conceptual mastery of fluid statics among students, employing a descriptive quantitative approach. The research involved 22 male and 13 female students from eleventh-grade MIPA at a private school in Surabaya, Indonesia. The primary research instrument comprised 11 multiple-choice and reasoning questions, covering concepts related to Pressure and Hydrostatic Pressure, Pascal’s Law, and Archimedes’ Principle. Results indicated a higher level of conceptual mastery among male students compared to their female counterparts. While both genders demonstrated relatively high conceptual mastery in Pressure and Hydrostatic Pressure, significant disparities were observed in their understanding of Pascal’s Law and …