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Effect Of Ethanol On Thermoregulation In The Goldfish Carassius Auratus, Candace Sharon O'Connor

Dissertations and Theses

In an attempt to elucidate the mechanism by which ethanol affects vertebrate thermoregulation, the effect of ethanol on temperature selection was studied in the goldfish, Carassius auratus. Ethanol was administered to 10 to 15 g fish by mixing it in the water of a temperature gradient. The dose response curve was very steep between 0.5% (v/v) ethanol (no response) and 0.7% (significant lowering of selected temperature in treated fish). Fish were exposed to concentrations of ethanol as high as 1.7%, at which concentration most experimental fish lost their ability to swim upright in the water. At concentrations higher than …

Use Of Electron Back Scatter Diffraction Patterns For Determination Of Crystal Symmetry Elements, D. J. Dingley, Karim Baba-Kishi

Scanning Electron Microscopy

The application of electron back scatter diffraction in the scanning electron microscope has been extended to the determination of crystal symmetry elements, point group and space group. The wide angular range of the patterns makes this a relatively simple task compared with equivalent analysis using electron channelling patterns, convergent beam patterns or standard x-ray methods, though the complexity of the analysis does not permit an unthinking approach. To establish the best procedure specimens from the seven crystal systems were investigated and results from the examination of the metal tin (tetragonal), and minerals zircon (ZrSiO₄, tetragonal) and calcite (CaCO …

Preparation Methods For Quantitative Electron Probe X-Ray Microanalysis Of Rat Exocrine Pancreas: A Review, N. Roos, T. Barnard

Scanning Electron Microscopy

Pancreatic acinar cells are thought to secrete a fluid containing digestive enzymes and electrolytes and use e,g, calcium as a second messenger upon stimulation, Together with their pronounced morphological polarity, they provide a model system to study the effect of different preparation methods for quantitative biological electron probe X-ray microanalysis (EPXMA) of ultrathin sections, Several preparation methods i,e,, freeze-drying and plastic-embedding, freeze-substitution (2 days) and freeze-drying of ultrathin cryosections have been applied to examine the retention of sodium, magnesium, phosphorus, sulfur, (chlorine), potassium and calcium in subcellular compartments (basal cytoplasm, apical cytoplasm, mitochondria and zymogen granules), In freeze-substituted samples the …

Corrosion Cast Technique Applied In Lymphatic Pathways, A. Castenholz

Scanning Electron Microscopy

The paper deals with methods and results of the microcorrosion cast technique in lymph angiology. For the representation of the special organization of the lymph vascular system including the initial vascular structures, intranodal pathways, bigger collectors, and lymph trunks, the application of various injection techniques is necessary. The interstitial injection of Mercox proves to be suitable to show the initial lymphatics and prelymphatic spaces. Similarly, the intranodal injection makes visible the system of the lymph sinuses and the spaces of the reticular tissue in this organ. Casts of bigger collecting vessels, lymph trunks, and thoracic duct can be obtained by …

The Study Of Biominerals By High Resolution Transmission Electron Microscopy, Stephen Mann

Scanning Electron Microscopy

This paper presents an overview of the study of the ultrastructure of biogenic inorganic solids (biominerals) using high resolution transmission electron microscopy (HRTEM). A range of biominerals have been studied including iron oxides, calcium phosphates, calcium carbonates and silica. The studies have revealed information concerning the structural complexity of these materials and have identified crystallographic order and disorder at the nanometre level. In addition, the results have aided the elucidation of the mechanisms of nucleation and growth of biogenic minerals.

Bird Use Of Lakes In The Claremont-Upland Area, Valerie Banschbach

Pomona Senior Theses

I studied water bird use of two lakes in the Claremont-Upland area to determine what physical, chemical, and biological aspects of these lakes provide suitable water bird habitat and food resources. I censused the Bernard Field Station Lake in Claremont and a gravel pit freshwater area in Upland from 10/85 to 3/86 for water bird use. I also mapped these sites, noted their water surface area and water depth changes, monitored their water chemistry, and censused their vegetation. The results of this work showed more total water birds per hour of observation time, and more birds per hour of observation …

Viruses In Water, Nasrin Mohammadpour Daryaee

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

The use of water by man, plants and animals is universal. Without it there can be no life. Every living thing requires water. Man can live nearly two months without food, but can live only three or four days without water. In our homes, whether in the city or in the country, water is essential for cleanliness and health. Water can be considered as the principal raw material and the lowest cost raw material from which most of our farm produce is made. It is essential for the growth of crops and animals and is a very important factor in …

Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protective antigen component of B. anthracis toxin was produced and purified to the >99% level. Toxin was purified from culture supernatant utilizing concentration and liquid chromatography techniques. Purity was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The purified protective antigen retained biological and antigenic activity as evidenced respectively by lethality in Fischer 344 rats when injected in combination with lethal factor, and by positive results on the Ouchterlony double diffussion assay.

Radioiodinated protective antigen was used both in the in vivo and the in vitro experiments.

In vivo distribution of labelled protective antigen was determined in Fischer 344 rats. Assay …

Life History And Production Of Three Species Of Isopods In Beargrass Creek Louisville, Ky, James A. Eckstein

Electronic Theses and Dissertations

The life history and annual production of the aquatic isopods L. fontinalis, C. intermedia and C. brevicaudus in a spring stream, Beargrass Creek, Louisville, Kentucky was studied. Reproductive activity was observed with the greatest intensity in the spring months from March to May. Smaller secondary peaks for recruitment of young were seen in the fall months for L. fontinalis and C. intermedia. Brood size for the three isopods was compared by linear regression. The mean annual biomass for the three isopods ranged from 0.67 g/m2 for C. intermedia, 0.83 g/m2 for C. brevicaudus to 2.1 g/m2 for L. fontinalis. Annual …

Detection Of Abnormal Milk With Impedance Microbiology Instrumentation, Fahad Ali Abdulghany Khayat

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mastitic milk was detected by obtaining conductance measurements using an impedance microbiology Bactometer® 120 SC instruments. Conductance readings taken after 30 min at 25'C separated normal and abnormal milks when readings differed by more than 3% from the variance among instrument module wells. Samples blended from four quarters of a cow indicated milk from one quarter was abnormal if the salt level in the abnormal quarter raised the blend conductivity above that of normal samples and variance among the wells. Either solid or liquid substrates that contained bacterial stimulants could be used to accelerate bacterial acid production or to reduce …

Contractile And Biochemical Properties Of Diaphragm: Effects Of Exercise Training And Fatigue, Joseph Mark Metzger, Robert H. Fitts

Biological Sciences Faculty Research and Publications

The effect of high-intensity trained (6 X 4.5 min at 40 m/min, 15% grade, 2.5-min rest between bouts, 5 days/wk, for 6 wk) on contractile, biochemical, and fatigue properties of the rat diaphragm were examined. The exercise program produced significant elevations in the mitochondrial marker enzyme citrate synthase (µmol·X·g^-1·X min^-1) in the soleus (SOL) (27.2 ± 1.5 vs. 46.7 ± 2.4; mean ± SE), deep vastus lateralis (DVL) (40.8 ± 2.6 vs. 58.3 ± 2.8), and superficial vastus lateralis (SVL) (8.5 +/- 0.6 vs. 11.4 +/- 0.7). No significant differences were observed in the crural (CRU) …

Effect Of High-Intensity Exercise Training On Functional Capacity Of Limb Skeletal Muscle, John Patrick Troup, Joseph Mark Metzger, Robert H. Fitts

Biological Sciences Faculty Research and Publications

The purpose of this study was to determine whether a program of regular sprint exercise training alters the functional properties or protects against the development of fatigue in fast- and slow-twitch rat skeletal muscle. The training program consisted of 6 sprints of 4.5-min duration at 40 m/min and 15% slope with 2.5-min rest intervals, performed 5 days/wk for 6 wk. The exercise program significantly increased (P-1·min^-1) in the predominantly type I soleus (SOL) from 28 ± 2 to 44 ± 2; the type IIb superficial region of the vastus lateralis (SVL) from 10 ± 1 to 16 ± …

Hormonal Control Of Phloem Loading In Celery (Apium Graveolens L.), Mark Watts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Previous research has indicated that phloem loading may be regulated by plant growth regulators. This conclusion, however, is based on circumstantial evidence in that previous experiments implemented complex tissue such as leaf discs or petioles to simulate phloem loading. Celery is a favorable system to measure phloem loading because it utilizes pure vascular tissue and/or isolated phloem tissue. Pure phloem tissue has not been used to date in phloem loading research.

It was demonstrated that the plant growth regulators gibberellic acid (GA₃) and indole acetic acid (IAA) caused an enhancement of sucrose-loading in vascular bundles and in isolated …

The Determination Of Wet Weight Concentrations Of Elements In Freeze-Dried Cryosections From Biological Cells, Karl Zierold

Scanning Electron Microscopy

Energy dispersive X-ray microanalysis in STEM (scanning transmission electron microscope) of freeze-dried cryosections from biological cells provides information on the subcellular element distribution in terms of dry weight concentration. The local dry weight content in the range of 5-50%, respectively the local water content within 50 to 95%, in different subcellular compartments can be determined by measuring the darkfield intensity by means of an annular detector in STEM. Calibration is done by measuring the darkfield intensity of similarly prepared cryosections from dextran-water-solutions in varying concentration. Thus, by combining the X-ray microanalytical data evaluated by the continuum method with the STEM …

Functional Aspects Of Renal Glomeruli Based On Scanning Electron Microscopy Of Corrosion Casts, With Special Emphasis On Reptiles And Birds, H. Ditrich, H. Splechtna

Scanning Electron Microscopy

The glomerular complexity of several species of birds and reptiles is investigated in this study by scanning electron microscopy of vascular corrosion casts. Comparing these results with those of a freshwater teleost and a mammalian species, a trend towards small, simple glomeruli of the avian type, beginning with large, well vascularized glomeruli resembling the type found in fish, can be observed in reptiles.

A close correlation between glomerular size and habitat can be established comparing related species having a similar physiological mode of renal function.

Entirely different from this sauropsidian evolutionary line of development is the highly complex, large differentiation …

Technical Parameters Of Plastics (Mercox Cl-2b And Various Methylmethacrylates) Used In Scanning Electron Microscopy Of Vascular Corrosion Casts, T. Weiger, A. Lametschwandtner, P. Stockmayer

Scanning Electron Microscopy

The most frequently used resins for vascular corrosion casting Mercox Cl-2B, Mercox Cl-2B diluted with methylmethacrylate (MMA) monomer and various self prepared MMA and hydroxyproyl-methacrylate mixtures were tested with regard to their thermostability, shrinkage, viscosity and replication quality. It was found that tempering of the plastics improves their thermostability with the exception of Mercox Cl-2B and that shrinkage depends on the amount of monomers a resin contains. In detail; Mercox Cl-2B has the lowest shrinkage (8.018%) whereas a hydroxypropyl-methacrylate mixture possessed the highest (20.408%). But, on the other hand, viscosity decreases with the quantity of monomers. All resins tested were …

Computer Simulation And Experimental Performance Data For An Electron Spectrometer For Electron Beam Testing Of Integrated Circuits, D. Deutges, S. Görlich, E. Kubalek

Scanning Electron Microscopy

Electron beam testing using voltage contrast in the scanning electron microscope has been established as a useful tool for nondestructive and nonloading functional testing and failure analysis of integrated circuits (IC). The accuracy of quantitative voltage measurements within the IC with the electron beam probe is determined by the performance of the secondary electron (SE) spectrometer used.

For simulating the performance of SE-spectrometers a program-package has been developed by aid of which the voltage-and field-distributions within the spectrometers can be evaluated using a finite element method. Thus it is possible to trace electron trajectories throughout the spectrometer. By considering a …

Scanning Electron Microscopic Studies On Microvascular Architecture Of Human Coronary Vessels By Corrosion Casts: Normal And Focal Necrosis, T. Ono, Y. Shimohara, K. Okada, S. Irino

Scanning Electron Microscopy

Microvascular architecture of the normal human heart and myocardial focal necrosis were studied by scanning electron microscopy of corrosion casts. Casts macroscopically identical in form to the left ventricular posterior wall were prepared.

The following results were obtained in the normal human heart. (I) Most of the arterioles communicated with capillary plexuses smoothly and straight forwardly in the left ventricular posterior free wall. (2) Arterioles which branched from the arteries ran in various directions and continued into capillaries either at right angles or obliquely in the trabeculae carneae. (3) capillaries running parallel with the cardiac muscle fibers ran in different …

Intensity Of Valence Auger Transitions (L23vv) Of Al And Si In Metal, Oxide And Nitride, M. Gautier, C. Quennisset, J. P. Duraud, J. P. Vigouroux, C. Le Gressus

Scanning Electron Microscopy

L₂₃VV Auger transition has been studied in Si, SiO₂, Al, AlN, Al₂O₃ by electron spectroscopy excited either by electron beam or X Rays. A strong difference is observed in intensity between pure solid and oxide or nitride under electron bombardment. Auger intensity is very sensitive to changes in the backscattering coefficient or inelastic mean free path. However transient local trapping of electrons seems to be responsible for the large change observed.

A Summary Of Ion Beam Etching Of Biological Material With Special Reference To The Saddle Field Source, D. Claugher

Scanning Electron Microscopy

A brief review of etching of biological materials using radio frequency, ion beam and electrostatic focused sources. Results of experiments using fast atom sources to dissect pollen, diatoms, protozoa and small arthropods, indicate that they produce a much smoother result which enables investigations to be carried out at high magnifications with the SEM.

Physical Basis For Spectrometer Calibration, M. Gautier, J. P. Duraud, J. P. Vigouroux, C. Le Gressus

Scanning Electron Microscopy

Progress in quantitative surface analysis is hampered by the lack of experimental procedure including spectrometer calibration, sample preparation, and general experimental setting-up. Two methods for spectrometer alignment are compared: the linearization method and the elastic peak test. Experimental spectra are presented, which can be considered as stringent reference data to check the instrument response and the analyser transmission at low energies.

Principles Of X-Ray Spectroscopy, Edwin R. Levin

Scanning Electron Microscopy

X-ray spectroscopy has become an essential tool for obtaining local chemical constituent data, particularly for identifying features observed in imaging systems. This tutorial paper is intended to introduce the basic ideas of X-ray spectroscopy, especially to the newcomer to scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The primary focus is on energy-dispersive analysis (EDS); however, the close parallels with wavelength-dispersive analysis (WDS) are shown. Topics include electron beam-specimen interactions, X-ray generation and detection, spectral analysis, approaches to quantitation, and limits of detection and resolution.

Theory For Calcium-Phosphate Crystal Formation In Tissue From Scanning Electron Microscope Data, Alan C. Nelson

Scanning Electron Microscopy

Scanning electron microscope (SEM) morphological analysis combined with energy dispersive characteristic x-ray analysis provides insight into the mechanism of biological mineralization. A time series of tissue micrographs and mineralization measurements can permit the determination of the mineralization kinetic behavior and is the basis upon which a computer model has been devised. The computer model is constructed from fundamental principles of crystal nucleation and precipitation theory. Various general forms of the model are tested against the laboratory data for goodness-of-fit using the least squares method, and two models are found to be acceptable. Both of the acceptable models involve inhibition of …

Mineral Recognition And Liberation Degree Measurements In Industrial Ore Processing, G. Bonifazi, P. Massacci

Scanning Electron Microscopy

This study aims at identifying calculation procedures and representation methods for identifying the mineral species present in a rock. The goal of the study is to identify: the characteristic spectrum for each mineral species identified in the ore; the main shape of the individual species; the preferential arrangement of the individual mineral species; the grain-size distribution of the individual mineral species and of the grains resulting from comminution processes; the degree of liberation for each mineral; and the degree of liberation of the comminution products obtained from the ores.