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The Ratio Of Heinz Body Formation In Different Hemoglobin Zurich Subjects, Yenya Hu

Masters Theses & Specialist Projects

Hemoglobin Zurich is a hemoglobin anomaly that results when one amino acid (histidine) is substituted by arginine at position 63 in the beta chain of hemoglobin molecules [β 63 His—Arg]. When Hemoglobin Zurich individuals are exposed to sulfonamide medication, their hemoglobins denature and subsequently form Heinz bodies which attach to the surface of the plasma membrane.

Four Hemoglobin Zurich family members were the subjects of the current study. They included a splenectomized female subject, non-splenectomized female and male subjects, and a non-splenectomized female member without Hemoglobin Zurich as the control. The results collaborate that splenectomy increases the number of erythrocytes …

A Modified Scheme For The Isolation And Enumeration Of Bacteria In Municipal Sewage Sludge, Kelly Ball

Masters Theses & Specialist Projects

Because of the potential health hazards associated with the use of sludge for agricultural purposes, Dudley et al (1980) published a scheme for the routine analysis of bacteria in municipal sewage sludge. In this study, the Dudley et al scheme (1980) was modified by updating some of the procedures. Aerobically digested sludge generated by the Bowling Green Wastewater Treatment Plant, Bowling Green, Kentucky, was analyzed using the modified scheme. Sludge samples were collected once every two months over a one-year period from October 1989 to August 1990.

Egg yolk-free tryptose sulfite cycloserine agar in conjunction with the revewrse CAMP test …

Intermediates In The Constitutive And Regulated Secretory Pathways Released In Vitro From Semi-Intact Cells, Mark L. Grimes, Regis B. Kelly

Biological Sciences Faculty Publications

Regulated secretory cells have two pathways that transport secreted proteins from the Golgi complex to the cell surface. To identify carrier vesicles involved in regulated and constitutive secretion, PC12 pheochromocytoma cells were labeled with [35S]sulfate to identify markers for the two secretory pathways, then mechanically permeabilized and incubated in vitro. Small constitutive secretory vesicles, containing mostly sulfated proteoglycans, accumulated during an in vitro incubation with ATP. In the presence of GTP gamma S, the constitutive vesicles became significantly more dense, suggesting that a coated intermediate was stabilized. Larger immature regulated secretory granules, enriched in sulfated secretogranin II, also escaped from …

Evaluating Habitat Suitability Using Relative Abundance And Fledging Success Of Red-Naped Sapsuckers, Bret W. Tobalske

Biological Sciences Faculty Publications

No abstract provided.

Genetic-Divergence Between The Wistar-Kyoto Rat And The Spontaneously Hypertensive Rat, Mark L. Johnson, Daniel Ely, Monte E. Turner

Biology Faculty Research

A method of restriction fragment length polymorphism (RFLP) analysis was used to estimate the amount of genetic divergence between the spontaneously hypertensive rat (SHR) strain and the Wistar-Kyoto (WKY) strain. DNA from each strain was digested with eight restriction endonucleases and hybridized with six single copy gene sequences. The number of hybridization bands in each digestion was used to estimate the total number of bases analyzed and RFLPs were scored as single mutations. Divergence was then estimated by dividing the number of mutations by the number of bases analyzed. In a total of 808 bases analyzed in WKY rats, a …

Augmentation Of Natural Populations Of Entomophagous Insects Through The Use Of Secondary Food Sources, John Swallow

Undergraduate Honors Capstone Projects

Local densities of predators, including ladybeetles, parasitoids, big-eyed bugs, and minute pirate bugs, increased with the application of artificial honeydew, consisted of sugar and wheast dissolved in water. Sugar, rather than wheast, was the active ingredient causing predators to aggregate, although wheast prolonged the effect of the sugar treatment for the ladybeetles. Furthermore, the artificial honeydew treatments had a marked effect for a period of one week. These results suggest a potentially important role of the use of synthetic honeydew as a means of pest control in an Integrated Pest Management system.

Circulating Antibodies To Thymic Antigens In Autism And Alzheimer's Disease, Chih-Li Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Abnormal T lymphocyte reactions in both autism and Alzheimer's disease (AD) have been reported. This research investigated the possibility that these abnormalities may involve circulating antithymic antibodies. Plasma samples from autistic patients, AD patients, and normal-matched controls were tested for reactivity against murine thymocytes.

In the first of 3 studies results of the enzyme-linked immunosorbent assay (ELISA) were statistically significant for binding (P < 0.001) between antithymic antibodies in plasmas of AD patients and murine thymocytes. Binding (P < 0.05) in low dilutions (1/2.5 and 1/5} of autistic patient plasmas was also observed. In the second study, plasmas of neither autistic nor AD patients significantly inhibited DNA synthesis of thymic cells in the presence of interleukin-1 (IL-l} and phytohemagglutinin (PHA). In the third study, no significant increases (P > 0.05) in cytotoxic activities were detected using AD patient plasmas and both untreated and heat-treated autistic patient plasmas. After further testing, these heat-treated plasmas diluted 1/64 and 1/128 had increased cytotoxicities (P < 0.05) when rabbit complement was added, an indication that cytotoxicity of antithymic antibodies is complement dependent.

Therefore, circulating antithymic antibodies may …

Biosystematics Of Seven Species Of Stipa From Southwestern United States And Northern Mexico, Penelope S. Hoge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two previously unidentified taxa of Stipa L. were evaluated morphologically, electrophoretically, and ecologically. The two 'new' taxa (tentatively referred to as "puzzlei" and "valdesii") were compared with five species from southwestern United States and northern Mexico (Stipa lobata Swallen, Stipa alta Swallen, Stipa scribneri Vasey, Stipa lettermanii Vasey, and Stipa nelsonii Scribner), all of which are considered to belong in the Achnatherum species-group. Anatomical and micromorphological features show that the 'new' taxa also belong in this species-group. Principal coordinate analyses of the morphological data showed that each of the seven taxa tended to occupy a …

The Influence Of Dietary Factors On The Risk Of Urinary Stone Formation, A. Hesse, R. Siener, H. Heynck, A. Jahnen

Scanning Microscopy

The action of various beverages and foods on the composition of the urine in the circadian rhythm and in the 24-hour urine has been investigated under standardized conditions. Orange juice leads to a significant increase of urinary pH and citric acid excretion. Black tea leads to a raised excretion of oxalic acid by only 7.9%. In the short term, beer increases diuresis, but afterwards leads to a compensatory antidiuresis with increased risk of stone formation. Depending on their composition, mineral waters have very different effects on the urinary constituents. Milk as well as cocoa beverage significantly increase calcium excretion; moreover, …

Particle Induced X-Ray Emission Microanalysis Of Root Samples From Beech (Fagus Sylvatica), M. Hult, B. Bengtsson, N. P. -O. Larsson, C. Yang

Scanning Microscopy

Beech seedlings (Fagus Sylvatica) were grown in a nutrient solution to which AlCl₃ had been added. The experiments were designed to evaluate the effect of aluminum on the localization of elements in plant root tissue. Cross-sections of roots were analyzed with micro-PIXE (particle induced X-ray emission) and Rutherford backscattering (RBS) simultaneously. A proton beam of either 30x30 μm² or 5x5 μm² was scanned over the samples, and quantitative elemental maps were created. Aluminum was found to accumulate in high concentrations (percent levels) in the epidermis and the outer layer of the cortex, when the nutrient …

Relationship Between Villous Shape And Mural Structure In Neutron Irradiated Small Intestine, K. E. Carr, J. S. Mccullough, A. C. Nelson, S. P. Hume, S. Nunn, H. H. M. Kamel

Scanning Microscopy

Previous work on irradiation of mouse small intestine has assessed the changes produced by counting crypts/microcolonies, scoring villous shape or examining morphological changes in specific parts of the wall.

This paper used scanning and transmission electron microscopy to study the effects of whole body irradiation with 5 Gy neutrons on the surface and internal features of the intestinal wall of CFLP mice, 1 day, 3 days and 7 days after treatment.

Empirical scores from the ultrastructural findings were inserted into a Morphological Index display calculated from analytical data based on cell counts and area measurements obtained from resin histology sections. …

An Improved Method For Preparing Microvascular Corrosion Casts Of Rat Embryos, S. Yoshida, J. Chiba

Scanning Microscopy

This paper presents an improved method for preparing microvascular corrosion casts of rat embryos (day 16.5-21.5). Special attention was paid on the viscosity of the casting material, the method of mechanical restraint, and the subsequent drying of the casts. The embryos were perfused with Mercox resin diluted with 25% methyl methacrylate monomer followed by undiluted Mercox resin to avoid outflow of resin from the vasculature after perfusion. The specimens were mounted on a stainless steel plate with self-curing resin to prevent flotation, mechanical damage, and collapse of the cast specimens following preparation. After digestion and washing, the cast specimens were …

Scanning Electron Microscopy And Energy-Dispersive X-Ray Microanalysis Studies Of Early Dental Calculus On Resin Plates Exposed To Human Oral Cavities, T. Kodaka, Y. Ohohara, K. Debari

Scanning Microscopy

Dental calculus formed after 10 days on resin plates, applied to the lingual sides of the mandibular gingival regions in eight human subjects, was investigated by means of scanning electron microscopy (SEM) and energy-dispersive X-ray microanalysis (EDX). The mineral deposits were mainly divided into three types: A, B, and C. The type A deposits showing an average Ca/P molar ratio of 1.42 were densely packed with fine needle-shaped crystals formed by the intra- and extracellular calcification. The type A deposits, probably composed of Ca-deficient apatites and the transitional forms between apatite and octacalcium phosphate (OCP), were observed in all subjects. …

A Review Of Graphite And Gold Surface Studies For Use As Substrates In Biological Scanning Tunneling Microscopy Studies, Carol R. Clemmer, Thomas P. Beebe Jr.

Scanning Microscopy

The current status of biological Scanning Tunneling Microscopy (STM) investigations and the importance of using a well-characterized substrate are discussed. The findings of over two years of experiments and over 1,000 images obtained on gold substrates prepared by a variety of different methods are statistically summarized and compared to a very flat reference substrate, highly oriented pyrolytic graphite (HOPG). In an effort to begin to corroborate STM results with those obtained from other more established techniques, the results of Auger Electron Spectroscopy (AES) and Electron Spectroscopy for Chemical Analysis (ESCA) of biomolecular STM samples are presented.

Can We See Living Structure In A Cell?, Gilbert N. Ling

Scanning Microscopy

Colloid chemistry (κολλα: glue, or gelatin) was introduced in 1861 after the discovery of protoplasm which exhibits gelatin-like properties. Some 80 years later, colloid chemistry (and with it, the concept of protoplasm) was largely abandoned. The membrane (pump) theory, according to which cell water and cell solute like K⁺ are free as in a dilute KCl solution, became dominant. Later studies revealed that rejecting the protoplasmic approach to cell physiology was not justified. Evidence against the membrane (pump) theory, on the other hand, has stood the test of time. In a new theory of the living cell called the …

The Scanning Probe Microscope, J. Jahanmir, B. G. Haggar, J. B. Hayes

Scanning Microscopy

Scanning probe microscopy bas evolved into a powerful tool since its inception in 1982. The scanning probe microscope bas found applications in metrology, spectroscopy, and lithography. We will review the background of the technology, discuss the different types of scanning probe microscopes including the scanning tunneling microscope and the scanning force microscope, and present many of the applications for the instrument.

A Comparison Of Benthic Invertebrate Distributions In Four Ohio River Basin Wetlands, Michael D. Hardin

Morehead State Theses and Dissertations

A thesis presented to the faculty of the Department of Biological and Environmental Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Michael D. Hardin on April 1, 1992.

Canid Elicitation Of Blood-Squirting In A Horned Lizard (Phrynosoma Cornutum), George Middendorf, Wade Sherbrooke

Department of Biology Faculty Publications

No abstract provided.

Thermal Properties Of Some Lipid Components Of Cell Membranes, Lihua Wei

Masters Theses & Specialist Projects

Phospholipids are the major structural components of the lipid portion of biological membranes. The study of the thermal properties of these phospholipid provides a systematic understanding of the relationships between the chemical structures and functional properties of phospholipid molecules in artificial and biological membranes. This study examined the thermal behavior of phospholipids in the solid state using DSC and TG coupled with FITR. The phospholipids were found to have phase transitions below the melting point. Egg-yolk lecithin had four transitions below its melting point; sphingomyelin had two phase transitions below its melting point. Phosphatidylethanolamine and phosphatidylserine had complex phase transitions. …

Correction For Extraneous Background In X-Ray Microanalysis Of Cell Cultures, Anne Von Euler, Romuald Wróblewski, Godfried M. Roomans

Scanning Microscopy

Some practical aspects of the X-ray microanalysis of cell cultures have been investigated. Cells were cultured on titanium grids covered with Formvar films and analyzed at 100 kV either in the scanning transmission (STEM) or transmission mode (TEM) of the electron microscope. Different holders, grids and configurations were compared with respect to the relative contribution of different factors to the extraneous background in the X-ray spectrum. When low atomic number holders are used, the contribution to the spectrum of electrons scattered through high angles, may be negligible. In practice this may result in negative values for the contribution of these …

Scanning Electron Microscopy - Energy Dispersive Spectroscopy And X-Ray Diffraction Analyses Of Human Salivary Stones, H. Mishima, H. Yamamoto, T. Sakae

Scanning Microscopy

Ten salivary stones in the human submandibular gland were investigated by scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and X-ray diffraction analyses. The stones usually showed a lamellar pattern. SEM observations revealed cubical, plate-like, granular, small and large granules, polyhedral or globular structures in these stones. By X-ray powder diffraction analysis, the main constituents of salivary stones were found to be apatite and whitlockite. SEM-EDS analyses showed that Ca and P were the major elements, frequently accompanied by Mg and S, and less frequently by Na, Al, Si, Cl, K, Fe, Cu and Zn. Ca/P molar ratios ranged from 1.00 to …

Scanning Tunneling Microscopy Of Biological Macromolecular Structures Coated With A Conducting Film, M. Amrein, H. Gross

Scanning Microscopy

We have studied the capability of scanning tunneling microscopy (STM) to reveal the three-dimensional structure of biological macromolecular structures that have been rendered conductive by metal-coating. The sample preparation used has been derived from a well established method in transmission electron microscopy (TEM). It includes adsorption, freezing and dehydration by vacuum-sublimation, followed by metal-shadowing of the specimen. As an alternative to adsorption and coating, fluid biomaterials can be replaced by conductive freeze-fracture replica.

We give an introduction into the sample preparation of metal-coated specimens and discuss how each step can affect the structural preservation and thereby the quality of the …

Visualizing Cells In Three Dimensions Using Confocal Microscopy, Image Reconstruction And Isosurface Rendering: Application To Glial Cells In Mouse Central Nervous System, Frank Morgan, Elisa Barbarese, John H. Carson

Scanning Microscopy

This paper describes a general method for visualizing individual cells in intact tissue in three dimensions. The method involves immunostaining intact tissue to label specific cells, "optical sectioning" the stained tissue by laser scanning confocal microscopy, computationally reconstructing a three dimensional image data set from the digitized confocal optical sections, delineating isosurfaces of specific intensity within the reconstructed image by a "marching cubes" algorithm to generate polygon meshes defining boundaries of cells, and displaying individual cells, identified as three dimensional objects enclosed by contiguous polygon meshes, using computer graphics techniques. Each of the components of this method has been described …

Growth Of Yba2cu3o7-Δ Thin-Films–Nucleation, Heteroepitaxy And Interfaces, M. Grant Norton, C. Barry Carter

Scanning Microscopy

A major research effort in high-temperature oxide superconductors is the growth of high-quality thin-films. The physical properties of polycrystalline thin-films are controlled by their microstructure which is influenced by the early stages of film growth and the establishment of epitaxy. In this article, the nucleation and heteroepitactic growth of YBa₂Cu₃O_7-δ thin-films is reviewed. Many of the examples illustrating film growth will be taken from studies using transmission electron microscopy. The importance of the substrate surface in controlling film growth will be discussed, in particular the growth of YBa₂Cu₃O_7-δ films on …

The Interpretation Of X-Ray And Electron Signals Generated In Thin Or Layered Targets, R. H. Packwood, G. Remond

Scanning Microscopy

This work outlines the development of a comprehensive theory for the electron probe microanalyser and scanning electron microscope or SEM, that is intended to serve as a framework of understanding for those employing electron beam methods and as a basis for improved correction procedures. There is particular emphasis on applications to layered and non-uniform specimens. Starting from a simple Gaussian depth distribution of electrons and making assumptions about the X-ray production, a series of predictions of X-ray and electron signals are made for various target configurations. When compared with experimental measurements a series of interesting discoveries follow, which, taken altogether, …

Improved Bacterial Baby Machine: Application To Escherichia Coli K-12, Charles E. Helmstetter, Cindy Eenhuis, Patrick W. Theisen, Julia E. Grimwade, Alan C. Leonard

Biomedical Engineering and Sciences Faculty Publications

Exponentially growing derivatives of Escherichia coli K-12 were immobilized onto the surfaces of nitrocellulose membrane filters which had been coated with poly-D-lysine. The cells attached firmly to the surfaces, and when flushed with culture medium, the immobilized cells continued to divide and newborn cells were released into the effluent. Cell cycle parameters were examined with the technique, and it was found that K-12 derivatives possessed differing values for interdivision times, C, D, and average cell sizes when grown in the same culture media. It was also found that the cells released from immobilized populations of one culture consisted of two …

Three Dimensional Mammalian Skull Morphology, Suzanne Louise Krippaehne

Dissertations and Theses

This thesis deals with archiving morphological data utilizing a three dimensional coordinate system. Morphological reference points are archived via rectangular position coordinates, rectangular position vectors, and spherical position vectors. The concepts of translation trajectories, translation vectors, and relative position vectors are developed. Analysis of three dimensional coordinate data utilizing translation trajectories and translation vectors is described. In order to test the methodology developed, the method is applied to an analysis of harbor porpoise, Phocoena phocoena L., skull morphology. (Key words: morphology, ontogenetic trajectories, allometry, position coordinates, position vectors, translation trajectories, translation vectors, relative position vectors, and harbor porpoise).

The Stability Of Adsorbates Imaged With A Scanning Tunneling Microscope Using Hopping Versus Constant Current Scanning, Terje G. Vold

Scanning Microscopy

We have studied the stability of various adsorbates, including gold, a platinum-iridium alloy, and DNA, on monoatomically flat gold imaged with a scanning tunneling microscope. We find that adsorbates are generally more stable, sometimes dramatically so, if imaged with a hopping trajectory of the tip rather than with the conventional constant-current scanning technique. Gold pits and associated debris formed on flat gold surfaces under saline solution by mechanical impact of the tip with the surface are always much more stable when imaged with hopping. Samples of thin, sub-monoatomic layers (0.1 nm and 0.2 nm thick) of a platinum-iridium alloy evaporated …

Image Processing Procedures For Analysis Of Electron Back Scattering Patterns, N. C. Krieger Lassen, D. Juul Jensen, K. Conradsen

Scanning Microscopy

At present, computer-aided Electron Back Scattering Pattern (EBSP) analysis often requires large amounts of operator time if statistically reliable measurements are needed. This paper presents ways to automatically detect and localize bands in EBSPs and thereby enable fully automatic EBSP analysis. The main focus will be on a procedure using a modified Hough transform by which more than 12 bands in a typical EBSP can be detected and localized. This procedure seems as effective and reliable for extracting the bands of EBSPs as any human operator. The performance of this procedure is compared with that of other image processing procedures.

Quantitative Imaging Ion Microscopy: A Short Review, G. A. Valaskovic, G. H. Morrison

Scanning Microscopy

A short review of recent efforts being made in the quantification of images in ion microscopy is given. Special aspects of instrumentation, detection and acquisition, which are unique to direct imaging secondary ion mass spectrometry, are discussed in relation to the successful application of traditional empirical quantification schemes. Application of such quantification schemes requires proper sample preparation, standardization, analysis, and quite often, special techniques in image processing and the correlation of ion microscopy with other microscopies. Quantification within this technique is a difficult goal which can only be realized if the analyst pays strict attention to every step of the …