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Regulation Of The Na+/H+ Antiport In Xenopus Laevis Oocytes During Meiotic Maturation, Katayoun Rezai

Master's Theses

No abstract provided.

Isolation And Analysis Of A Cdna From The Gag/Protease Region Of The Soybean Retrotransposon, Sire-1, Yi-An Bi

Master's Theses

No abstract provided.

Regulatory Factors Affecting Nitrate Reductase In Soybean, Glycine Max, Donna Santucci

Master's Theses

No abstract provided.

Comparison Of Stomach Contents Of Smallmouth Bass Collected By Sport Fishermen And Electroshocking, Lyne Marie Ross-Darrus

Master's Theses

No abstract provided.

Pcr Cloning And Sequencing Of A Cdna Coding For A Nitrate Reductase Isoform From Soybean, Glycine Max, James Schavocky

Master's Theses

No abstract provided.

Molecular Phylogenetic Studies On Filarial Parasites Based On 5s Ribosomal Spacer Sequences, Hong Xie, O. Bain, Steven A. Williams

Biological Sciences: Faculty Publications

This paper is the first large-scale molecular phylogenetic study on filarial parasites (family Onchocercidae) which includes 16 spe­ cies of 6 genera : Brugia beaveri Ash et Little, 1964 ; B. buckleyiDissanaike et Paramananthan, 1961 ; B. malayi (Brug, 1927) Buckley, 1960; B. pahangi (Buckley et Edeson, 1956) Buckley, 1960; B. patei (Buckley, Nelson et Heisch, 1958) Buckley, 1960; B. timori Partono etal, 1977; Wuchereria bancrofti(Cobbold, 1877) Seurat, 1921; W. kalimantani Palmieri , Purnomo, Dennis and Marwoto, 1980; Mansonella perstans(Manson, 1891 ) Eberhard et Orihel, 1 9 8 4 ; Loa loa, Stiles, 1905; Onchocerca volvulus (Leuckart, 1983) Railliet et …

Cellular Mechanisms Of Muscle Fatigue, Robert H. Fitts

Biological Sciences Faculty Research and Publications

Fatigue, defined as the failure to maintain the required or expected power output, is a complex problem, since multiple factors are clearly involved, with the relative importance of each dependent on the fiber type composition of the contracting muscles(s), and the intensity, type, and duration of the contractile activity. The primary sites of fatigue appear to be within the muscle cell itself and for the most part do not involve the central nervous system or the neuromuscular junction. The major hypotheses of fatigue center on disturbances in the surface membrane, E-C coupling, or metabolic events. The cell sites most frequently …

Development And Application Of A Thermistor Current Meter, Carl M. Way, Albert J. Burky, Christine Miller-Way

Biology Faculty Publications

This report provides details for the construction of a hot-bead thermistor current meter which is capable of measuring water velocities on a millimeter spatial scale and for the construction of a compact and accurate calibration system. Hot-bead thermistor current meters can be built with response times of 200 ms capable of measuring velocities between 0.1 and 80 cm s-1. The construction of a sturdy probe for application in lotic systems such as high gradient Hawaiian streams was achieved by the use of heavy-duty acrylic tubing, small stainless steel gas-chromatography tubing, and flexible Tygon spaghetti tubing. An acrylic handle anchors the …

Characterization And Genetic Analysis Of A Pigmentation Mutant Of Neurospora Crassa, Craig West Banks

College of Graduate Studies: Theses & Dissertations (1964–2006)

The mutant overaccumulator of carotenoids, ovc. was obtained from the Fungal Genetics Stock Center in Kansas City, Kansas. This mutant had been isolated following UV irradiation of the colonial mutant col-4 and mapped to Linkage Group IV (R. Harding, 1984 Neurospora Newsletter 34: 23-25). Examination of ovc in our laboratory revealed that in addition to having increased pigmentation, the mutant was osmotic sensitive, failing to grow on 6% NaCl. Morphology of ovc closely resembles that of cut, another osmotic sensitive mutant in LG IV. A cross of ovc to the osmotic sensitive mutant cut failed to produce …

The Role Of Sexual Selection In Structuring West Indian Passerine Communities, C. Lynne Starling

College of Graduate Studies: Theses & Dissertations (1964–2006)

Patterns of plumage dimorphism were examined among species of passerines native to 19 West Indian islands. Based on sexual selection theory, the predictions were made that dimorphic species: (1) occur on fewer and larger islands than monomorphic species due to reduced colonization ability and reduced survivorship imposed by sexual selection pressure, and (2) should be added to island communities disproportionately as area increases, an idea which was tested using linear models.

No significant difference was observed between number of islands or minimum island area occupied by dimorphics versus monomorphics. The traditional ANCOVA model best fits the data and suggests that …

Susceptibility Of Eumeces Species Skinks To A Southeastern Isolate Of The Lyme Disease Spirochete (Borrelia Burgdorferi), Gregory A. Cummins

College of Graduate Studies: Theses & Dissertations (1964–2006)

The SI-4 strain of Borrelia burgdorferi obtained from field collected Ixodes scapularis on Sapelo Island, Georgia, was infective to seven of eight cotton mice (Peromyscus gossypinus) by needle inoculation. I. scapularis larvae were then fed on them, and nymphs molting from these larvae were then fed on laboratory mice, Mus musculus, in an attempt to transmit the spirochete. One of three mice was successfully infected; confirmation of the presence of B. burgdorferi was made using BSK II culture and the polymerase chain reaction (PGR). A subsequent attempt was made to determine if SI-4 was infective for Eumeces …

Spiroplasma (Entomoplasmatales) Associated With Tabanidae (Diptera) And Lampyridae (Coleoptera), Jimmy Wedincamp Jr.

College of Graduate Studies: Theses & Dissertations (1964–2006)

Abstract 1:

To determine the exit points of Spiroplasma from tabanids, 45 naturally infected Tabanus gladiator and 6 T. sulcifrons were restrained and fed 10% sucrose. The flies were allowed to feed for 24 hours and the resulting oral and anal specks were cultured in MID broth. Spiroplasmas were isolated from 21 of 51 oral specks but none of 23 anal specks deposited on plastic. In contrast, when anal specks were deposited in a sucrose solution, 9 of 28 anal specks in sucrose yielded spiroplasma cultures. Air-dried spiroplasma cultures in 5% sucrose survived for up to 30 days in the …

Reaction Norms For Age And Size At Maturity In Response To Temperature: A Puzzle For Life Historians., D. Berrigan, Eric Charnov

Biology Faculty & Staff Publications

Ectotherms mature later at smaller size when growth rates are lowered by reductions in food quality but they mature later at larger sizes when growth rate is lowered by reductions in temperature. We argue that this general pattern has been neglected by life history theorists and suggest that an explanation for these strikingly different responses to two environmental cues might be found by considering correlations between the growth coefficient of the Bertalanffy equation and asymptotic size in a model for the evolution of age and size at maturity.

Morphology, Fluid Motion And Predation By The Scyphomedusa Aurelia Aurita, John H. Costello, Sean Colin

Arts & Sciences Faculty Publications

Although medusan predators play demonstrably important roles in a variety of marine ecosystems, the mechanics of prey capture and, hence, prey selection, have remained poorly defined. A review of the literature describing the commonly studied medusa Aurelia aurita (Linnaeus 1758) reveals no distinct patterns of prey selectivity and suggests that A. aurita is a generalist and feeds unselectively upon available zooplankton. We examined the mechanics of prey capture by A. aurita using video methods to record body and fluid motions. Medusae were collected between February and June in 1990 and 1991 from Woods Hole, Massachusetts and Narragansett Bay, Rhode Island, …

Purification, Characterization, And Submitochondrial Localization Of The 32-Kilodalton Nadh Dehydrogenase From Maize, Andrew F. Knudten, Jay J. Thelen, Michael Hans Luethy, Thomas Elthon

School of Biological Sciences: Faculty Publications

Plant mitochondria have the unique ability to directly oxidize exogenous NAD(P)H. We recently separated two NAD(P)H dehydrogenase activities from maize (Zea mays 1.) mitochondria using anion-exchange (Mono Q) chromatography. The first peak of activity oxidized only NADH, whereas the second oxidized both NADH and NADPH. In this paper we describe the purification of the first peak of activity to a 32-kD protein. Polyclonal antibodies to the 32-kD protein were used to show that it was present in mitochondria from several plant species. Two-dimensional gel analysis of the 32-kD NADH dehydrogenase indicated that it consisted of two major and one minor …

Characterization Of The Unique Intron - Exon Junctions Of Euglena Gene(S) Encoding The Polyprotein Precursor To The Light-Harvesting Chlorophyll A/B Binding Protein Of Photosystem Ii, Umesh Shyamsunder Muchhal, Steven D. Schwartzbach

School of Biological Sciences: Faculty Publications

The precursor to the Euglena light harvesting chlorophyll a/b binding protein of photosystem 11 (LHCPII) Is a polyprotein containing multiple copies of LHCPII covalently joined by a decapeptide linker. cDNA and genomic clones encoding the 5 and 3' end of a 6.6 kb LHCPII mRNA were sequenced. A 3.1 kb genomic region encoding 1.05 kb of the 5' end of LHCPII mRNA contains 4 introns. A 7.6 kb genomic region encoding 3.3 kb of the 3' end of LHCPII mRNA contains 10 introns. The 5' and 3' ends of the 14 Identified Euglena introns lacked the conserved dinucleotides (5'-GT and …

Enhanced Competitiveness Of Tomato Bushy Stunt Virus Defective Interfering Rnas By Segment Duplication Or Nucleotide Insertion, K. Andrew White, T. Jack Morris

School of Biological Sciences: Faculty Publications

We have analyzed atypical tomato bushy stunt virus defective interfering (DI) RNA species which accumulated during a passage series in protoplasts. We present a rationale for the order of appearance of these molecules and show, using competition assays, that either segment duplication or single nucleotide insertion can enhance DI RNA competitiveness. Possible mechanisms for the introduction of the modifications observed in these DI RNAs are discussed.

The Effect Of High Intensity Exercise Training Verses Low Intensity Exercise Training On Fractionated Plasma High-Density Lipoprotein Cholesterol, Teresa L. Spate

Masters Theses

Exercise training is thought to result in an elevation of high-density lipoprotein (HDL) and the cardioprotective subfraction HDL{dollar}\sb2{dollar}. Twenty-five healthy women (39 {dollar}\pm{dollar} 7.8 yrs; 70 {dollar}\pm{dollar} 14 kg) walked two miles three times per week for 12 weeks to examine the effect of exercise intensity on the HDL profile. The L.I. group (N = 12) walked at 60% of the heart rate reserve (HRR) and the H.I. group (N = 13) walked at 80% HRR both maintaining the prescribed distance and frequency. A 22% increase in total HDL (from 32 {dollar}\pm{dollar} 6 to 39 {dollar}\pm{dollar} 8 mg/dl) and a …

Identification Of Tire Leachate Toxicants And A Risk Assessment Of Water Quality Effects Using Tire Reefs In Canals, S. M. Nelson, G. Mueller, D. C. Hemphill, U.S. Bureau Of Reclamation

Publications (WR)

Cover is an important component of aquatic habitat and fisheries management. Fisheries biologists often try to improve habitats through the addition of natural and artificial material to improve cover diversity and complexity. Habitat-improvement programs range from submerging used Christmas trees to more complex programs using sophisticated artificial habitat modules. Used automobile tires have been employed in the large scale construction of reefs and fish attractors in marine environments and to a lesser extent in freshwater and have been recognized as a durable, inexpensive and long-lasting material which benefits fishery communities.

Recent studies by the U.S. Bureau of Reclamation have quantified …

Terrapene Carolina Carolina, Joseph C. Mitchell, Rafael O. De Sá

Biology Faculty Publications

TERRAPENE CAROLINA CAROLINA (Eastern Box Turtle). REPRODUCTION. Female Terrapene carolina containing oviductal eggs have been recorded in Virginia from 26 May to 25 July (Mitchell, in press. The Reptiles of Virginia. Smithsonian Inst. Press, Washington, D.C.). Other reports of reproduction in Terrapene (see list in Ernst and McBreen 1991. Cat. Amer. Amphib. Rept. 512.1-512.13) indicate that nesting occurs in late spring and early summer throughout its range.

What Are Squirrels Hiding, Michael Steele, Peter D. Smallwood

Biology Faculty Publications

The half-eaten acorns of squirrels are clues to their complex relationship with oaks. The special relationship between squirrels and acorns is examined.

Ecology Of The Spotted Turtle, Clemmys Guttata At The Western Range Limit, Thomas P. Wilson

Masters Theses

No abstract provided.

The Origins Of The Neutral Theory Of Molecular Evolution, Michael Dietrich

Dartmouth Scholarship

No abstract provided.

Per-Like Immunoreactive Neurons Within The Brain Of Xenopus Innervate Both The Retina And Pineal Organ, J. M. Garcia-Fernandez, Kathleen King Siwicki, R. G. Foster

Biology Faculty Works

No abstract provided.

Innervation Patterns In Neuromasts Of The Mottled Sculpin, Cottus Bairdi, Jie He

Master's Theses

No abstract provided.

Morphometry Of Growth In Hatchling Striped Plateau Lizards (Iguanidae: Sceloporus Virgatus), Geoffrey R. Smith

Faculty Publications

No abstract provided.

New And Interesting Records Of Birds From A Dry Forest Reserve In South-West Ecuador, Karl S. Berg

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

La reserva forestal Cerro Blanco es una de las pocas áreas protegidas con más de 200 ha de bosque seco sobre el oeste ecuatoriano. Aunque existen listas de registros de aves en la reserva, con frecuencia no se conocen las fechas ni localidades en que fueron tomados. De otro lado, esta información no incluye observaciones de algunas de las partes de más difícil acceso en la reserva, ni muestra con precisión las diferentes altitudes, los tipos de bosques o las estaciones. Una información más amplia será cada vez más importante para la conservación y manejo de la vida silvestre de …

Effects Of Ionizing Radiation On The Size Distribution Of Proteoglycan Aggregates Synthesized By Chondrocytes In Agarose, M. Cornelissen, H. Thierens, L. De Ridder

Scanning Microscopy

Effects of ionizing radiation on the structure and size-distribution of newly synthesized proteoglycan aggregates are studied in-vitro. Chondrocytes, isolated from embryonic chick sterna, are cultured for 7 days in a tri-dimensional agarose system. Single radiation doses of 10, 20 and 50 Gy are given before starting the culture. Digestion of the artificial agarose matrix liberates the newly synthesized proteoglycans. Spreading with cytochrome C allows electron microscopic investigations of the individual, newly synthesized molecules.

The structure of aggregates synthesized by control and irradiated chondrocytes is comparable. However, radiation causes alterations in the size-distributions of the aggregate-populations. For the control cultures, an …

Investigation Of Dislocations In Gaas Using Cathodoluminescence In The Scanning Electron Microscope, K. L. Pey, J. C. H. Phang, D. S. H. Chan

Scanning Microscopy

Electrically active dislocations in Si-doped {100} GaAs substrates were observed using the cathodoluminescence (CL) technique in the scanning electron microscope (SEM). CL contrast profiles were experimentally obtained from the dislocations at different beam energies. Based on the CL model for localized defects in semiconductors developed earlier by Pey, the depths of the dislocations were found by locating the beam energy at which maximum CL contrast occurred. A preferential etching technique for {100} GaAs was employed to reveal the dislocations and to measure their depths. The etched depths obtained were compared to the predicted results from the theoretical model developed. The …

Imaging-Sims (Secondary Ion Mass Spectroscopy) Studies Of Advanced Materials, R. Levi-Setti, J. M. Chabala, J. Li, K. L. Gavrilov, R. Mogilevsky, K. K. Soni

Scanning Microscopy

This article describes the application of scanning ion microprobe (SIM) microanalysis for the characterization of advanced engineered materials. In conjunction with secondary ion mass spectrometry (SIMS), scanning ion microprobes can image elemental distributions over surfaces with high lateral resolution (50-100 nm). With this technique, most elements, including isotopes, can be detected with good sensitivity. The principles and instrumentation associated with the SIM/SIMS technique are briefly described and ongoing developments are outlined. The analytical capabilities of the technique are illustrated by case studies of aluminum-lithium alloys, zinc oxide varistors, aluminum matrix composites, and photographic materials.