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Cell Surface Changes Of Hemopoietic Cells During Normal And Leukemic Differentiation: An Immuno-Scanning Electron Microscopy Study, D. Soligo, E. P. De Harven, N. Quirici, L. Caneva, G. Lambertenghi Deliliers

Scanning Microscopy

Hemopoietic cells display a wide range of cell surface antigens which are either lineage specific or acquired during differentiation. Monoclonal antibodies can be used, in conjunction with colloidal gold markers, to identify under the scanning electron microscopy (SEM) at the single cell level, specific lineage or maturation stages in the hemopoietic bone marrow. Normal bone marrow cells, either gradient separated or purified by immuno-magnetic methods and leukemic cell samples, which can be considered as "frozen" stages of hemopoietic differentiation, have been studied with this method. Typical cell surface morphologies, which characterize immature progenitor cells and cells committed or differentiated towards …

Etiology Of Calcium Oxalate Nephrolithiasis In Rats. I. Can This Be A Model For Human Stone Formation?, W. C. De Bruijn, E. R. Boevé, P. R. W. A. Van Run, P. P. M. C. Van Miert, R. De Water, J. C. Romijn, C. F. Verkoelen, L. C. Cao, F. H. Schröder

Scanning Microscopy

Crystal retention is studied in a rat-model system as a possible mechanism for the etiology of human nephrolithiasis. A crystal-inducing diet (CID) of ethylene glycol plus NH₄Cl in their drinking-water is offered to healthy rats to generate intratubular crystals. Subsequently, the fate of retained crystals is investigated by allowing the rats a tissue recovery/crystalluria phase for three, five and ten days, respectively, on normal drinking water.

The process of exotubulosis is observed in cortex and medulla of aldehyde-fixed kidneys after three days recovery. After five days, crystals are predominantly seen there in the interstitium. After ten days, cortex …

Etiology Of Calcium Oxalate Nephrolithiasis In Rats. Ii. The Role Of The Papilla In Stone Formation, W. C. De Bruijn, E. R. Boevé, P. R. W. A. Van Run, P. P. M. C. Van Miert, R. De Water, J. C. Romijn, C. F. Verkoelen, L. C. Cao, J. M. Van 'T Noordende, F. H. Schröder

Scanning Microscopy

In kidneys of healthy rats submitted to a crystal-inducing diet (CID) with ethylene glycol (EG) and NH₄Cl, the fate of retained crystals in the papillar region is studied during a recovery period of one, five or ten days, as model system for human nephrolithiasis. Scanning electron microscopy (SEM) shows, at papillary tips bulging into the calycine space, crystal masses covered either by the epithelium or a thin fibrous veil, or by unidentified mobile cuboidal cells. After CID plus one or five days recovery, small sub-epithelial swellings are seen of large sub-epithelial crystals at or around the papillary tip. …

Dynamic Diseases In Neurology And Psychiatry, John Milton, Deborah Black

WM Keck Science Faculty Papers

Thirty-two (32) periodic diseases of the nervous system are identified in which symptoms and/or signs recur. In 10/32, the recurrence of a symptom complex is one of the defining features of the illness, whereas in 22/32 oscillatory signs occur in the setting of an ongoing nervous system disorder. We discuss the possibility that these disorders may be dynamic diseases.

Characterization Of A Saccharomyces Cerevisiae Strain Deleted For The Rad27 Gene; A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Wolfram Siede, Errol C. Friedberg

Biology Faculty Publications

No abstract provided.

Dynamical Disease: Identification, Temporal Aspects And Treatment Strategies For Human Illness, Jacques Bélair, Leon Glass, Uwe An Der Heiden, John Milton

WM Keck Science Faculty Papers

Dynamical diseases are characterized by sudden changes in the qualitative dynamics of physiological processes, leading to abnormal dynamics and disease. Thus, there is a natural matching between the mathematical field of nonlinear dynamics and medicine. This paper summarizes advances in the study of dynamical disease with emphasis on a NATO Advanced Research Workshop held in Mont Tremblant, Quebec, Canada in February 1994. We describe the international effort currently underway to identify dynamical diseases and to study these diseases from a perspective of nonlinear dynamics. Linear and nonlinear time series analysis combined with analysis of bifurcations in dynamics are being used …

Draft Report Of The South Coast Estuarine Fishery Working Group, South Coast Estuarine Fishery Workng Group

Fisheries Management Papers

The South Coast Estuarine Working Group was established in 1991 at the request of commercial fishermen by the Minister of the day. This report outlines a draft management plan for the South Coast Estuarine fishery that was made available for public comment.

Enhancer Trap Technique: A Novel Tool For Identification And Developmental Characterization Of Genes Of Drosophila, Amit Singh

Biology Faculty Publications

The classical technique of mutational screen for identification of genes controlling early development has now approached saturation. A new era in genetic identification and developmental characterization of genes in Drosophila has commenced with the advent of the enhancer trap technique. This technique involves mobilization of a P-lacZ vector to diverse chromosomal locations in the fruit fly genome to bring it under the regulation of developmentally expressed genes or their enhancer elements. The technique offers a strikingly elegant method of gaining entry into fruit fly genes.

Reproductive Structure And Organogenesis In A Cottonwood, Populus Deltoides (Salicaceae), Robert B. Kaul

School of Biological Sciences: Faculty Publications

The organogenesis of inflorescences, flowers, and fruits was followed for two years in a male and a female tree of eastern cottonwood, Populus deltoides. Soon after anthesis, an inflorescence for the next year is initiated as a continuation of the apical meristem in most axillary buds of the extension shoot of the current year. Bract and then floral primordia arise helically, and by the end of summer all floral appendages are evident. Individual perianth parts are evident early in ontogeny but not at anthesis; they are vascularized independently by distal traces of discrete vascular strands that also serve the …

Review Of "Correspondence: Karl Ernst Von Baer [1792-1876] And Anton Dohrn [1840-1909]" By C. Groeben And J. Oppenheimer, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Notes On The Microstructure Of The Nautilus Shell, P. R. Mitchell, P. P. Phakey

Scanning Microscopy

The shell of the Nautilus was examined using scanning electron microscopy, transmission electron microscopy and polarized light microscopy. The structure consisted of two major layers: a porcellaneous outer layer and a nacreous inner layer. Most of the porcellaneous layer was composed of granular crystals randomly distributed with a substructure suggestive of bundles of acicular crystallites. A separate prismatic sublayer of the porcellaneous material was composed of a more regular arrangement of crystals. The nacre was composed of alternating crystalline lamellae and films of organic material. The lamellae were formed of many polygonal crystal platelets. The growth surface of the nacre …

Zooplankton Community Structure In Lakes Near Mt. St. Helens, Wa, Larry Duane Scharnberg

Dissertations and Theses

Eighteen lakes around Mt. St. Helens (MSH) were sampled for zooplankton from September '92 until September '94. Samples were enumerated and identified to the species level in most cases. Standard deviation and t-tests were performed to determine the precision of enumeration methods and replication of duplicate tows. Palatability indexes based upon predator preferences were developed and coupled with length-frequency analyses as indicators of predation pressure. The weighted means of the summer samples were then subjected to cluster analysis in an attempt to categorize lakes with respect to zooplankton community structure. Lastly, the community compositions and abundances of MSH lakes were …

Investigation Of Nanoparticles In High Resolution Scanning Electron Microscopy (Sem) And Low Voltage Sem By Digital Image-Analysis, B. Ocker, R. Wurster, H. Seiler

Scanning Microscopy

Small particles (Cu, Ag, In, Sn, Au, also MgO and NaCl) were prepared in the diameter range from 1 nm to 100 nm on different conductive substrates by thermal evaporation in high-vacuum or in an inert gas atmosphere. Imaging of the particles was performed in a high resolution scanning electron microscope (HRSEM) that can also be operated at low beam voltages of a few hundred volts. This mode of operation is called low voltage SEM (LVSEM). Scanning electron micrographs were taken at different beam voltages V_O (0.5-30 kV). The micrographs were digitally recorded and analyzed with an image processing …

High Resolution Electron Microscopy Of Enamel Crystallites Demineralized By Initial Dental Caries, Yoshihiko Hayashi

Scanning Microscopy

Acids produced by various oral bacteria cause mineral loss and crystallite dissolution during the development of enamel caries. In order to demonstrate this phenomenon, the initial disappearance of lattice fringes and the formation of a central perforation in crystallites were examined by high resolution electron microscopy (HREM) in initial enamel caries without macroscopic tissue evidence of destruction. Ultrathin sections were also examined by selected area electron diffraction to reveal the mineral phase of the surface layer in carious enamel.

A marked variation in the dissolution pattern was disclosed in the initial carious lesions. HREM revealed that disappearance of the lattice …

Controlling Influence Of Phosphocitrate In Vitro And In Vivo On Calcium Oxalate Crystal Formation And Growth, John D. Sallis, Nathan F. G. Parry, John D. Meehan, Harold Kamperman, Maree E. Anderson

Scanning Microscopy

Calcium oxalate (CaOx) crystallization in the presence of phosphocitrate (PC) was studied by both in vitro and in vivo techniques. Crystals of the monohydrate (COM) and the dihydrate (COD) forms were generated under controlled conditions in a silica gel matrix. Our data indicated only COD crystals formed when PC was present, inferring that the COD to COM transformation was being impeded. COD crystals were smaller in size than controls and there was evidence of interpenetral twinning. An in vivo study using a rat bladder implant model noted similar findings. Scanning electron microscopy (SEM) revealed that implants recovered from PC treated …

The Biogeochemistry Of Lakes In The Mount St. Helens Blast Zone, Victor Lee Menting

Dissertations and Theses

Dilution and ash weathering are the most important processes controlling the ion chemistry of lakes in the Mount St. Helens blast zone. Gibbs' models indicated total dissolved solids were decreasing as a result of dilution from high precipitation and runoff and the lakes plot in the rock weathering dominated region. Plots of theoretical dilution curves indicated a decline in ion concentrations as a result of dilution. Ion concentrations followed the exponential decline predicted by the dilution curve, although concentrations were higher than predicted by the curve. Increased concentrations were a result of the rapid weathering of ash in basins and …

Community Metabolism And Nutrient Cycling In The Mississippi River Plume: Evidence For Intense Nitrification At Intermediate Salinities, Joseph D. Pakulski, Ronald Benner, Rainer Amon, Brian Eadie, Terry Whitledge

Faculty Publications

Community respiration, net nutrient fluxes and heterotrophic bacterial production were investigated in the Mississippi River (USA) plume during May 1992 using dark bottle incubations of unfiltered water. Highest rates of community O₂ consumption and dissolved inorganic carbon regeneration were observed at intermediate (10 to 27%₀) plume salinities. Plume surface 0₂ consumption rates were 2- to 4-fold greater than rates reported previously during the summer and winter. Heterotrophic bacterial production ([³H]-leucine incorporation) was also highest at intermediate salinities and 2- to 4-fold greater than rates reported from other seasons. Net regeneration of NH₄^{+ …}

Reply To Nylin, Wickman & Wiklund Regarding Sex Ratios Of California Overwintering Monarch Butterflies, Dennis F. Frey, Kingston L. H. Leong

Biological Sciences

No abstract provided.

Phylogenetic Reanalysis Of Strauch's Osteological Data Set For The Charadriiformes, Philip C. Chu

Biology Faculty Publications

Strauch's (1978) compatibility analysis of relationships among the shorebirds (Charadriiformes) was the first study to examine the full range of charadriiform taxa in a reproducible way. Subsequently Mickevich and Parenti (1980) leveled serious charges against Strauch's characters, method of phylogenetic inference, and results. To account for these charges, Strauch's characters were re-examined and recoded, and parsimony analyses were performed on the revised matrix. A parsimony analysis on 74 taxa from the revised matrix yielded 855 shortest trees, each length = 286 and consistency index = 0.385. In each shortest tree there were two major lineages, a lineage of sandpiper-like birds …

Implications Of Native Title Legislation For Fisheries Management And The Fishing Industry In Western Australia, Patricia Summerfield

Fisheries Management Papers

This paper seeks to examine the key issues of both State and Commonwealth native title legislation for the fishing industry and fisheries management in Western Australia. Considerations for Aboriginal involvement in the industry and long-standing as well as recent concessionary arrangements for traditional usage of fish resources are examined. Industry reactions and future plans to accommodate native title in a positive framework are explored.

An Improved Method For Chemical Devitellinization Of X-Gal Stained Drosophila Embryos, Amit Singh, Madhuri Kango-Singh, P. Sinha

Biology Faculty Publications

In Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yieldirrg technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives …

Richard Goldschmidt's "Heresies" And The Evolutionary Synthesis, Michael Dietrich

Dartmouth Scholarship

No abstract provided.

Abundance And Diversity Of Aquatic Birds On Two South Texas Oxbow Lakes., Diane Teter, David L. Mcneely

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Avian use of two oxbow lakes located in urban surroundings of south Texas was studied for one year. Both abundance and diversity of aquatic birds were notably reduced in the more intensely developed area. Possible factors resulting in these differences are reviewed.

Revascularization Of An Excisional Wound In Gingiva And Oral Mucosa. A Scanning Electron Microscopic Study Using Corrosion Casts In Rats, Nils J. Selliseth, Knut A. Selvig

Scanning Microscopy

The purpose of this study was to examine microvascular regeneration associated with gingival wound healing. A full-thickness piece of gingiva and oral mucosa was excised along the palatal aspect of the right maxillary first and second molars in 20 young Wistar rats. The contralateral side served as unoperated control. After 2, 4, 7, 10 or 20 days of healing, microvascular corrosion casts were produced and examined by scanning electron microscopy. At 2 days, vessels surrounding the wound were dilated and impressions representing sites of leukocyte margination were prominent in the walls of venules. Capillary buds were emerging from venules and …

Scanning Electron Microscopy And Transmission Electron Microscopy Aspects Of Synergistic Antitumor Activity Of Vitamin C - Vitamin K3 Combinations Against Human Prostatic Carcinoma Cells, Jacques Gilloteaux, James M. Jamison, Meenaxi Venugopal, David Giammar, Jack L. Summers

Scanning Microscopy

A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K₃ (Vit K₃), or vitamin C:vitamin K₃ combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K₃ alone exhibited antitumor activity, but only at elevated doses. When Vit C and Vit K3 were combined at a C:K₃ ratio of 100:1 and administered to the carcinoma cells, the 50% cytotoxic concentrations (CD50) of the vitamins decreased 10-to 60-fold. Subsequently, the DU145 cells were examined with transmission and scanning electron microscopy (TEM and SEM) following …

Experimental Calcium Oxalate Nephrolithiasis And The Formation Of Human Urinary Stones, Saeed R. Khan

Scanning Microscopy

Calcium oxalate nephrolithiasis in rats requires induction of hyperoxaluria which results in increased urinary calcium oxalate supersaturation. As a result of low to mild chronic hyperoxaluria, calcium oxalate crystals deposit first in the papillary collecting ducts. Crystal deposition in the kidneys is preceded by calcium oxalate crystalluria and starts with the retention of aggregated calcium oxalate crystals in the renal tubules. Retained crystals move from the tubules to the interstitium, and in the process, become anchored to the tubular basement membrane. Crystal aggregates present in the superficial peripheral collecting ducts of the renal papillae ulcerate through to the papillary surface …

Cathodoluminescence Of Rare Earth Doped Zircons. I. Their Possible Use As Reference Materials, F. Cesbron, P. Blanc, D. Ohnenstetter, G. Rémond

Scanning Microscopy

Synthetic zircon crystals (ZrSiO₄), undoped and doped with Y³⁺, La³⁺, Ce³⁺, Pr³⁺, Nd³⁺, Sm³⁺, Eu³⁺, Gd³⁺, Tb³⁺, Dy³⁺, Ho³⁺, Er³⁺, Tm³⁺, Yb³⁺, Lu³⁺, were grown from a flux consisting of a mixture of Li₂MoO₄ and MoO₃ heated to 1125°C and then cooled to 750°C. The cathodoluminescence (CL) spectra of these zircons were analyzed at room-temperature and near liquid nitrogen temperature with a CL spectrometer …

Luminescence From Porous Silicon, Yasunori Mochizuki

Scanning Microscopy

Structural and optical properties of porous Si are reviewed with the main emphasis on the radiative recombination mechanisms. Behaviors of the visible photoluminescence and another intense luminescence process (the infrared luminescence) are discussed based on the available data provided by photoluminescence and related techniques. It is demonstrated that a further insight into the interrelation of these luminescence processes can be obtained by the optically-detected magnetic resonance method, in which non-radiative point defects (surface dangling bonds) are used as a local structural probe for the radiative states.

As for the model for the visible light emission, the implication of the quantum …

Diamond Luminescence, R. Heiderhoff, L. J. Balk

Scanning Microscopy

Luminescence spectroscopy is an established tool to investigate natural, high pressure synthesized, and chemical vapour deposited (CVD) diamond. The spectral range extends from 5.3 eV in the ultraviolet to approximately 1.2 eV in the near-infrared. More than 100 optical centres have been observed.

Since the early 1930's, semiconducting diamond for electronic devices has been of interest to science. The large bandgap (5.5 eV), low dielectric constant (5.7), and high thermal conductivity (about 5 times larger then that of Ag), as well as the superior charge-carrier transport properties, such as electron and hole mobility (µ^-: 2200 cm²/Vs, …

Temperature-Dependent Cathodoluminescence Spectroscopy And Microscopy As A Tool For Defect Identification In Semiconducting Ceramics: Application To Batio3 Ceramics, T. Hübner, U. Marx, J. Schreiber

Scanning Microscopy

Cathodoluminescence (CL) spectroscopy and microscopy were applied to investigate the characteristic grain-boundary contrast in semiconducting ferroelectric BaTiO₃ ceramics. It was shown, that "chemically clean" grain boundaries do not reveal any specific CL components neither in the visible nor in the infrared part of the spectrum. Instead, the contrast arises from at least two different non-radiative recombination centers present in the grain and the grain-boundary zones, respectively. Activation thresholds for these centers were determined from the temperature dependence of the integral CL signal down to 30K. The different values found explain the contrast reversal observed in BaTiO₃ ceramics upon …