Digital Commons Network^™

Cloning And Sequencing Of Sodium-Hydrogen Exchanger In The Gills Of The Long-Horned Sculpin (Myoxocephalus Octodecimspinosus), Danielle Lee Gunning

College of Graduate Studies: Theses & Dissertations (1964–2006)

No abstract provided.

Improving The Quality Of Foliage Transplants For Supply To The Kerry Foliage Industry, Gwen Lanigan

Theses

The possibility of improving the quality of transplants of ornamental foliage species, Eucalyptus perriniana, E. parvifolia and Pittosporum tenuifolium, for supply to the Kerry Foliage Industry was investigated. At present transplants (seedlings) are propagated from seed under glass and subsequently transplanted into the field. Therefore nursery practices for seed propagation, such as optimum sowing date, pot and medium selection, irrigation and fertilisation regimes, were investigated and optimised. The possibility of eliminating the occurrence of J- roots, a major problem with Eucalyptus species was also investigated. Vegetative propagation methods, such as cutting propagation and in vitro micro-propagation were investigated for comparative …

First Nesting Record Of Blue Jay (Cyanocitta Cristata) In Hidalgo County, Texas, Timothy Brush

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

No abstract provided.

Diet Of Gambelia Wislizenii From Chihuahua, Mexico, J. A. Lemos-Espinal, Geoffrey R. Smith, H. M. Smith, R. E. Ballinger

Faculty Publications

No abstract provided.

Effects Of Age And Group Size On Habitat Selection And Activity Level In Xenopus Laevis Tadpoles, D. R. Golden, Geoffrey R. Smith, Jessica E. Rettig

Faculty Publications

No abstract provided.

Application Of A Polymerase Chain Reaction-Elisa To Detect Wuchereria Bancrofti In Pools Of Wild-Caught Anopheles Punctualatus In A Filariasis Control Area In Papua New Guinea, Moses J. Bockarie, Peter U. Fischer, Steven A. Williams, Peter A. Zimmerman, Lysaght Griffin, Michael P. Alpers, James W. Kazura

Biological Sciences: Faculty Publications

Chemotherapy-based eradication programs are aimed at stopping transmission of Wuchereria bancroftiby its obligatory mosquito vector. This study compares one year post-treatment W. bancrofti infection rates of Anopheles punctulatus, the main vector of lymphatic filariasis in Papua New Guinea, using traditional dissection techniques and a polymerase chain reaction (PCR)-based ELISA of a parasite-specific Ssp I repeat. A total of 633 mosquitoes in 35 batches were dissected. Six batches contained W. bancrofti-infected mosquitoes, giving a minimum infection rate of 0.9%. This value was not different than the actual infection rate, which was 9 (1.4%) of 633 mosquitoes (P� 0.48). The DNA was …

Studying The Expression Of A Novel Microsatellite Mrna In Chironomous Riparius, Mina Ranzurmal

Seton Hall University Dissertations and Theses (ETDs)

The expression of a unique mRNA containing a microsatellite sequence was studied in C. riparius using differential display. First, the rnicrosatellite fragment was sequenced using Sanger's Sequencing method. The microsatellite cDNA contained 11 "ACA" repeats, 12 "AC" repeats, and 5 "CTC" repeats. Second, the affects of cadmium and heat shock on rnicrosatellite mRNA expression were studied. In cadmium experiments, larvae were exposed to low cadmium concentrations (0, 0.04, 0.4, and 4.0 mM) for 24 and 48 h and high cadmium concentrations (0, 1.0, 2.0, 4.0, 8.0, and 16.0 mM) for 48 h. The effects of cadmium on fed larvae at …

Analysis Of Genetic Factors Affecting High Density Lipoprotein Levels And Apoall Levels In C57 B1/6j And Fvb/N Wildtype Mice Using A Qtl Mapping Approach, Judith Barrios

Seton Hall University Dissertations and Theses (ETDs)

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Field Notes On The Biology Of The Long-Wattled Umbrellabird Cephalopterus Penduliger In West Ecuador., Karl S. Berg

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

La biología del Pajaro Paraguas Longipendulo Cephalopterus penduliger es quizas lo menos conocido del genero. A pesar de eso, lo consideraron una de las especies más amenzadas en el continente Sudamericano debido a la deforestación, caza y a un grado menor, la comercialización de las aves como mascotas. Entre febrero y octubre de 1998 yo observe C. penduliger a lo largo del período y 1.600 horas de observación en la Estación Biológica Jatun Sacha Bilsa, Provincia de Esmeraldas, Ecuador. Documente varios aspectos de las observaciones incluyendo un sonogramo de dos vocalizaciones, descripciones de comportamiento, forrageo y las primeras fotos de …

The Effects Of Low Ph On Sunfish Assemblages In Southeast Georgia, William Boyd Tate

College of Graduate Studies: Theses & Dissertations (1964–2006)

No abstract provided.

Use Of Oak Hammocks By Neotropical Migrant Songbirds During Stopover In Coastal South Carolina, Scott Glen Somershoe

College of Graduate Studies: Theses & Dissertations (1964–2006)

Many species of Neotropical migrant songbirds have been reported to be declining in the last few decades, and the loss and fragmentation of stopover habitat has been implicated as a potential cause of these population declines. To manage properly a fragmented forest landscape for migrating songbirds information is needed on how the size and the habitat of forest fragments influence their use by migrants during stopover. Therefore, I conducted censuses of Neotropical migrant birds in eight oak hammocks of varying size (0.32-3.08 ha) at the Savannah National Wildlife Refuge, Jasper County, South Carolina. Censuses were conducted at 0600-1030 h during …

Identification Of Potential Molecular Biomarkers Of Cadmium Induced Stress In C̲Hironomus Tentans ̲, David Hunter Dyer

Dissertations and Theses @ UNI

The objective of this research was to identify potential molecular biomarkers by looking for differences in the mRNA expression patterns between cadmium exposed and non-exposed freshwater aquatic invertebrate larvae. In the future, genes that indicate sublethal damage to a population at risk could be used to monitor the degradation or regeneration of an ecosystem. For this study, laboratory reared Chironomus tentans were placed in a sublethal concentration of cadmium treated water and the genes turned on in response to the cadmium were isolated from a cDNA subtract library. Six genes were identified and two potential biomarkers of environmental stress, ferritin …

Structure And Function Analysis Of Lin-14, A Temporal Regulator Of Postembryonic Developmental Events In Caenorhabditis Elegans, Yang Hong, Rosalind C. Lee, Victor Ambros

Dartmouth Scholarship

During postembryonic development of Caenorhabditis elegans, the heterochronic gene lin-14 controls the timing of developmental events in diverse cell types. Three alternativelin-14 transcripts are predicted to encode isoforms of a novel nuclear protein that differ in their amino-terminal domains. In this paper, we report that the alternative amino-terminal domains of LIN-14 are dispensable and that a carboxy-terminal region within exons 9 to 13 is necessary and sufficient for in vivo LIN-14 function. A transgene capable of expressing only one of the three alternativelin-14 gene products rescues a lin-14 null mutation and is developmentally regulated by lin-4. …

Photoperiodic And Pineal Modification Of The Effect Of 6-Methoxybenzoxazolinone On Deciduoma Growth In Rats, Everett S. Nixon

Electronic Dissertations and Theses

The compound 6-methoxybenzoxazolinone (6-MBOA) is a nonestrogenic compound that enhances reproduction. Decidualization, marked by the decidual cell reaction, is a process that causes the transformation of the uterine stromal cells into decidual cells. The purpose of the study was to determine the reproductive role of 6-MBOA during decidualization in rats exposed to a short photoperiod (8L: 16D), and a long photoperiod (16L: 8D). Decidualization was induced by surgical uterine scratch trauma unto the anti-mesometrial surface of the uterine epithelium on day 4 of pseudopregnancy (PPG) a reproductive state that precedes and converges into decidualization. Rats were treated with subcutaneous doses …

A Comparison Of Hsp70 Expression And Thermotolerance In Adults And Larvae Of Three Drosophila Species, Robert A. Krebs

Biological, Geological, and Environmental Faculty Publications

Heat shock proteins (Hsps) and other molecular chaperones perform diverse physiological roles. One is to facilitate, in part, organismal thermotolerance, of which the functional consequences depend on Hsp70 concentration and developmental stage in Drosophila melanogaster. To test whether an Hsp70-thermotolerance relationship is a general phenomenon within Drosophila, I assayed Hsp70 concentration at a range of temperatures in intact larvae and adults of three species, D. melanogaster, D. simulans, and D. mojavensis, and compared those results to the increase in survival to heat shock that occurs after an Hsp70 inducing pretreatment. Larvae of D. melanogaster and D. simulans responded similarly to …

Late Quaternary Fossil Mammals And Last Occurrence Dates From Caves At Barahona, Puerto Rico, Donald A. Mcfarlane

WM Keck Science Faculty Papers

Puerto Rico supported at least five genera of endemic terrestrial mammals in the late Quaternary, all of which are extinct. Whether these animals died out in the late Pleistocene, the mid-Holocene, or in post-Columbian time has not been established. This paper is the first attempt at radiometrically dating the 'last occurrences' of these taxa, together with the first unambiguous descriptions of localities reported by previous workers. Last occurrence dates for Nesophontes, Elasmodontomys and Heteropsomys are shown to be mid-Holocene and overlap with Amerindian occupation of the island. Acratocnus is known only from the late Pleistocene. No Puerto Rican taxon has …

Abalone Aquaculture In Western Australia : Policy Guideline., Fisheries Western Australia.

Fisheries Management Papers

Experimental or commercial abalone aquaculture is being pursued in many countries with major investments in The United States, Taiwan, China and Korea as well as in Australia. The combined total abalone aquaculture production from China and Taiwan was reported to be in the order of 2,000 tonnes and continues to rise. As cultured ‘cocktail’ abalone are below the legal harvest size of wild abalone, there will be limited competition between aquaculture and the wild fisheries.

Molecular Evidence For A New Bacteriophage Of Borrelia Burgdorferi, Christian H. Eggers, D. Scott Samuels

Biological Sciences Faculty Publications

We have recovered a DNase-protected, chloroform-resistant molecule of DNA from the cell-free supernatant of a Borrelia burgdorferi culture. The DNA is a 32-kb double-stranded linear molecule that is derived from the 32-kb circular plasmids (cp32s) of the B. burgdorferi genome. Electron microscopy of samples from which the 32-kb DNA molecule was purified revealed bacteriophage particles. The bacteriophage has a polyhedral head with a diameter of 55 nm and appears to have a simple 100-nm-long tail. The phage is produced constitutively at low levels from growing cultures of some B. burgdorferi strains and is inducible to higher levels with 10 mu …

Monogenic Control Of Iris Coloration In The January Tetra (Hemigrammus Hyanuary Characidae), Jack Frankel

Department of Biology Faculty Publications

The January tetra (Hemigrammus hyanuary Durbin) exhibits two eye color phenotypes. These have a silver iris, which is characteristic of the species, and a green color variant. Segregation patterns observed in the progenies from 12 different crosses support an hypothesis for the monogenic inheritance of iris coloration in this species.

Black-Tailed Deer Habitat Changes In A Portion Of The Mount St. Helens Blast Zone, Ronald W. Davis

All-Inclusive List of Electronic Theses and Dissertations

This study utilized Landsat TM imagery to evaluate black-tailed deer habitat changes in a portion of the Mount Saint Helens blast zone in western Washington. The purpose of this study was to compare the post eruption vegetation changes with population trends for black tailed deer. The 55,000-acre study area comprises a Washington Department of Game Management unit (GMU 524) located to the northnorthwest of Mount St. Helens. Approximately 80% ofthe mostly forested study area was affected by lateral blast from the 1980 eruption. The area is predominantly private land managed for timber production but a small portion falls within the …

Use Of Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction (Rapd-Pcr) To Distinguish North American Populations Of Acroptilon Repens (L.) Dc, Raul A. Ruiz

Theses and Dissertations - UTB/UTPA

Sixty U.S. populations of Acroptilon repens collected in 1998 were analyzed using RAPD-PCR. Each population was represented by five specimens gathered from each location. A total of 301 specimens were examined using two Operon Technologies, RAPD-PCR primers (A 10 and C04). The DNA amplification products produced through the PCR process were electrophoretically separated to produce DNA fragments ranging from 100 to 2000 base-pairs in length. The 60 U.S. populations sampled, produce ten RAPD banding pattern types using A 10 primer and seventeen pattern types with C04. Genetic variation within and among the populations was low, however, one dendogram did show …

Survey Of Amphibians And Reptiles In Powhatan County, Virginia With Selected Natural History Notes, Jason Daniel Gibson

Theses & Honors Papers

This thesis establishes baseline data on the herpetofauna that exist in Powhatan county. No systematic study of the amphibians and reptiles in Powhatan county has ever been conducted. The methods used in this survey include hand capture, egg mass observations, road kill collections, and frog call surveys. Documentation of each species collected included measurements, photographs, range maps, and tape recordings.

This survey produced 8 species of salamanders, 11 anurans, 6 turtles, 4 lizards, and 14 snakes. Based on records in the Atlas of Amphibians and Reptiles of Virginia (Mitchell & Reay, 1999), 11 new county records were discovered. This brings …

Transcriptional Up-Regulation Of The Cyclin D2 Gene And Acquisition Of New Cyclin-Dependent Kinase Partners In Human T-Cell Leukemia Virus Type 1- Infected Cells, Francisco Santiago, Elizabeth Clark, Siewyen Chong, Carlos Molina, Fariba Mozafari, Renaud Mahieux, Masahiro Fujii, Nazli Azimi, Fatah Kashanchi

Department of Biology Faculty Scholarship and Creative Works

Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent for adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis. Tax1 is a 40-kDa phosphoprotein, predominantly localized in the nucleus of the host cell, which functions to transactivate both viral and cellular promoters. It seems likely that HTLV-1, through expression of the viral regulatory protein Tax1, provides some initial alteration in cell metabolism predisposing the development of ATL. Here, we demonstrate that HTLV-1 infection in T-cell lines and patient samples causes overexpression of an early G1 cyclin, cyclin D2. The transcriptional up-regulation of the cyclin D2 gene is due …

The Return Of The Wild, John Jahoda

Bridgewater Review

No abstract provided.

Phylogeography Of The Night Lizard, Xantusia Henshawi, From Southern California, Robert Edward Lovich

Loma Linda University Electronic Theses, Dissertations & Projects

The granite night lizard, Xantusia henshawi, is a highly specialized crevice dwelling lizard found in the Peninsular Ranges of southern California, United States and northern Baja California, Mexico. Generally, little morphological variation is seen in this species over a variety of habitats throughout its range, from elevations near sea level to approximately 2000 meters, and from Mediterranean habitats near the Pacific coast to arid desert habitat in the Colorado Desert of California. One exception is the variation seen in the subspecies X h. gracilis, which differs from X h. henshawi in morphology, allozymes, ecology, and natural history.

In …

Three-Dimensional Structure Of A Complex Between The Death Domains Of Pelle And Tube, Tsan Xiao, Par Towb, Steven A. Wasserman, Stephen R. Sprang

Biological Sciences Faculty Publications

The interaction of the serine/threonine kinase Pelle and adaptor protein Tube through their N-terminal death domains leads to the nuclear translocation of the transcription factor Dorsal and activation of zygotic patterning genes during Drosophila embryogenesis. Crystal structure of the Pelle and Tube death domain heterodimer reveals that the two death domains adopt a six-helix bundle fold and are arranged in an open-ended linear array with plastic interfaces mediating their interactions. The Tube death domain has an insertion between helices 2 and 3, and a C-terminal tail making significant and indispensable contacts in the heterodimer. In vivo assays of Pelle and …

Nutrient Cycling In The Water Column Of A Subtropical Seagrass Meadow, Susan Ziegler, Ronald Benner

Faculty Publications

The cycling of nutrients was studied over a 16 mo period to determine how processes occurring between the water column and benthos influenced nutrient dynamics in a Thalassia testudinum dominated seagrass meadow. Nutrient concentrations were low and ranged from below detection to 0.59 µM ammonium (NH₄⁺), 0.04 to 0.29 µM nitrate plus nitrite (NO₃^- + NO₂^-), and below detection to 0.22 µM soluble reactive phosphate (SRP). Water column and benthic fluxes of NO₃^- + NO₂^- and SRP were usually below detection. The benthic fluxes of NH₄^{+ …}

Evolutionary And Acclimation-Induced Variation In The Heat-Shock Responses Of Congeneric Marine Snails (Genus Tegula) From Different Thermal Habitats: Implications For Limits Of Thermotolerance And Biogeography, Lars Tomanek, George N. Somero

Biological Sciences

Heat stress sufficient to cause cellular damage triggers the heat-shock response, the enhanced expression of a group of molecular chaperones called heat-shock proteins (hsps). We compared the heat-shock responses of four species of marine snails of the genus Tegula that occupy thermal niches differing in absolute temperature and range of temperature. We examined the effects of short-term heat stress and thermal acclimation on the synthesis of hsps of size classes 90, 77, 70 and 38 kDa by measuring incorporation of (35)S-labeled methionine and cysteine into newly synthesized proteins in gill tissue. Temperatures at which enhanced synthesis of hsps first occurred …

The Edaphic Factor And Patterns Of Variation In Lasthenia Californica (Asteraceae), Nishanta Rajakaruna, Bruce A. Bohm

Biological Sciences

Transectional studies of Lasthenia californica in the Jasper Ridge Biological Preserve (Stanford University) have documented the existence of two races (A and C) based upon flavonoid chemistry, achene morphology, allozymes, and flowering time differences. The two races coexist on a serpentine outcrop and have maintained a sharply defined pattern of distribution for a period of at least 15 yr. The present study has revealed significant differences in the physical and chemical features of the soils harboring the two races. Soils at the lower ends of the transects, where race A plants grow, have higher pH, cation exchange capacity, relative water …

Allometric Scaling Of Production And Life-History Variation In Vascular Plants, Brian Enquist, Geoffrey West, Eric Charnov, James Brown

Biology Faculty & Staff Publications

A prominent feature of comparative life histories is the well documented negative correlation between growth rate and life span. Patterns of resource allocation during growth and reproduction reflect life-history differences between species. This is particularly striking in tropical forests, where tree species can differ greatly in their rates of growth and ages of maturity but still attain similar canopy sizes. Here we provide a theoretical framework for relating life-history variables to rates of production, dM/ dt, where M is above-ground mass and t is time. As metabolic rate limits production as an individual grows, dM=dt ~M3=4. Incorporating interspecific variation in …