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Predation Rates On Real And Artificial Nests Of Grassland Birds, William Davison, Eric Bollinger Jan 2000

Predation Rates On Real And Artificial Nests Of Grassland Birds, William Davison, Eric Bollinger

Faculty Research & Creative Activity

We estimated nesting success at real and artificial nests of grassland birds to test the influence of nest type, nest position, and egg size on predation rates. We distributed wicker nests and realistic woven-grass nests baited with a clay egg and either a Northern Bobwhite (Colinus virginianus) egg or a House Sparrow (Passer domesticus) egg in four grasslands that were part of the Conservation Reserve Program in east-central Illinois. Nesting success averaged 86.5% for 12 days of exposure for artificial nests. For real nests, nesting success was markedly lower, averaging 39% over the entire nesting cycle and 59% during approximately …

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Season And Distance From Forest - Old Field Edge Affect Seed Predation By White-Footed Mice, Scott Meiners, James Mccormick Jan 2000

Season And Distance From Forest - Old Field Edge Affect Seed Predation By White-Footed Mice, Scott Meiners, James Mccormick

Faculty Research & Creative Activity

We studied the spatial pattern of seed predation across a forest-old field edge in both fall and winter to assess the potential for seed predators to influence plant spatial patterns. We used a 100 x 100 m grid that began 30 m inside the forest and extended 60 m into the old field. Inside this grid we placed seed stations at regular 10 m intervals and monitored seed removal. Seed predation varied significantly across the edge gradient in both fall and winter with the highest rate of seed removal at the edge in both trials. The spatial pattern of seed …

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Evaluation Of The Performance Enhancement Of Silicone Biofouling-Release Coatings By Oil Incorporation, Kathryn Truby, Christina Wood, Judith Stein, James Cella, John Carpenter, Christopher Kavanagh, Geoff Swain, Deborah Wiebe, David Lapota, Anne Meyer, Eric Holm, Dean Wendt, Celia Smith, Jean Montemarano Jan 2000

Evaluation Of The Performance Enhancement Of Silicone Biofouling-Release Coatings By Oil Incorporation, Kathryn Truby, Christina Wood, Judith Stein, James Cella, John Carpenter, Christopher Kavanagh, Geoff Swain, Deborah Wiebe, David Lapota, Anne Meyer, Eric Holm, Dean Wendt, Celia Smith, Jean Montemarano

Biological Sciences

In response to increased evidence of ecosystem damage by toxic antifouling paints, many researchers have developed nontoxic silicone fouling release coatings. The fouling release capability of these Systems may be improved by adding nonbonding silicone oils to the coating matrix. This idea has been tested by comparing the adhesion strength of hard- and soft-fouling organisms on a cured polydimethylsilicone (PDMS) network to that of the same network containing free polydi-methyldiphenylsilicone (PDMDPS) oil at five exposure sites in North America and Hawaii. Fouling coverage is discussed, together with the bioadhesion data, to emphasize that although these coatings foul the fouling is …

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New Specimens Of Late Quaternary Extinct Mammals From Caves In Sanchez Ramirez Province, Dominican Republic, Donald A. Mcfarlane, Abel Vale, Keith Christenson, Joyce Lundberg, Gabriel Atilles, Stein Erik Lauritzen Jan 2000

New Specimens Of Late Quaternary Extinct Mammals From Caves In Sanchez Ramirez Province, Dominican Republic, Donald A. Mcfarlane, Abel Vale, Keith Christenson, Joyce Lundberg, Gabriel Atilles, Stein Erik Lauritzen

WM Keck Science Faculty Papers

During the late Quaternary, the island of Hispaniola supported one of the most diverse mammalian faunas in the West Indies. Much of this diversity was lost to extinction in the past 100,000 years, but the timing of these events is poorly known. Here we report the paleontological findings of a multidisciplinary investigation of caves in the central Dominican Republic. These findings include new 'last occurrence' dates for the rodents Isolobodon portoricensis and Brotomys cf. voratus that take these genera to the dawn of the historic era; a first record of a last-interglacial sloth, and the first report of the upper …

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Structure Of A Rat Α1-Macroglobulin Receptor-Binding Domain Dimer, Tsan Xiao, Dianne L. Decamp, Stephen R. Sprang Jan 2000

Structure Of A Rat Α1-Macroglobulin Receptor-Binding Domain Dimer, Tsan Xiao, Dianne L. Decamp, Stephen R. Sprang

Biological Sciences Faculty Publications

α-Macroglobulin inhibits a broad spectrum of proteinases by forming macromolecular cages inside which proteinases are cross-linked and trapped. Upon formation of a complex with proteinase, α-macroglobulin undergoes a large conformational change that results in the exposure of its receptor-binding domain (RBD). Engagement of this domain by α-macroglobulin receptor permits clearance of the α-macroglobulin: proteinase complex from circulation. The crystal structure of rat α1-macroglobulin RBD has been determined at 2.3 Å resolution. The RBD is composed of a nine-stranded β-sandwich and a single α-helix that has been implicated as part of the receptor binding site and that lies on …

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Evidence For Microbial Fe(Iii) Reduction In Anoxic, Mining-Impacted Lake Sediments (Lake Coeur D’Alene, Idaho), David E. Cummings, Anthony W. March, Benjamin Bostick, Stefan Spring, Frank Caccavo Jr., Scott Fendorf, Frank Rosenzweig Jan 2000

Evidence For Microbial Fe(Iii) Reduction In Anoxic, Mining-Impacted Lake Sediments (Lake Coeur D’Alene, Idaho), David E. Cummings, Anthony W. March, Benjamin Bostick, Stefan Spring, Frank Caccavo Jr., Scott Fendorf, Frank Rosenzweig

Biological Sciences Faculty Publications

Mining-impacted sediments of Lake Coeur d'Alene, Idaho, contain more than 10% metals on a dry weight basis, approximately 80% of which is iron. Since iron (hydr)oxides adsorb toxic, ore-associated elements, such as arsenic, iron (hydr)oxide reduction may in part control the mobility and bioavailability of these elements. Geochemical and microbiological data were collected to examine the ecological role of dissimilatory Fe(III)-reducing bacteria in this habitat. The concentration of mild-acid-extractable Fe(II) increased with sediment depth up to 50 g kg−1, suggesting that iron reduction has occurred recently. The maximum concentrations of dissolved Fe(II) in interstitial water (41 mg liter …

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Biospheric Cooling And The Emergence Of Intelligence, David Schwartzman, George Middendorf Jan 2000

Biospheric Cooling And The Emergence Of Intelligence, David Schwartzman, George Middendorf

Department of Biology Faculty Publications

The long-term cooling history of the Earth's biosphere implies a temperature constraint on the timing of major events in biologic evolution, e.g., emergence of cyanobacteria, eucaryotes and Metazoa apparently occurred at times when temperatures were near their upper growth limits. Could biospheric cooling also have been a necessary condition for the emergence of veterbrates and their encephalization? The upper temperature limit for vertebrate growth is about 10 degrees below the limit for Metazoa (50 degrees C). Heterothermy followed by full homeothermy was likely a necessary condition for greater encephalization because of the energy requirement of larger brains. The temperature differential …

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A Survey Of Organochlorine Pesticide Contamination In Costa Rican Wildlife, Mark Wieland '00 Jan 2000

A Survey Of Organochlorine Pesticide Contamination In Costa Rican Wildlife, Mark Wieland '00

Honors Projects

Amphibians, turtles, rodents, and birds collected from a tropical conservation area in northwestern Costa Rica where pesticides are not directly applied were analyzed for organochlorine (OC) pesticide contamination. Six of thirty-nine amphibians (three of eight species), three of six turtles (two species), one of eight rodents (one species), and nine of twenty-five insectiverous birds (four species) contained OC compounds ranging from 2.77 ng/g to 277.70 ng/g. The most frequently detected compound (found in thirteen organisms) was p,p'-DDE. Heptachlor, delta-BHC, dieldrin, endosulfan II, and p,p' -DDD were each found in four or more organisms, while eight additional OC compounds were detected …

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Mainstreaming Feminist Critique Into The Biology Curriculum, Scott F. Gilbert, Biology And Gender Study Group Jan 2000

Mainstreaming Feminist Critique Into The Biology Curriculum, Scott F. Gilbert, Biology And Gender Study Group

Biology Faculty Works

No abstract provided.

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Aeromycological Spore Loads At A Newly Activated Compost Facility, Steven R. Mcgreevy Jan 2000

Aeromycological Spore Loads At A Newly Activated Compost Facility, Steven R. Mcgreevy

Honors Theses, 1963-2015

Composting facilities, because of the processes that occur there, may be associated with increased fungal spore loads in the air. We monitored the spore loads present at a newly activated composting facility (Mississippi Topsoils, Inc.; Cold Spring, MN) before and after its activation during October and November of 1999. Petri dishes were arrayed on the downwind side of the compost site and exposed for a range of time periods. The exposed petri dishes were incubated for 48 hours and the number of yeast-like, filamentous, and total fungal colonyforming-units (CFUs) was recorded. The spore load data were also compared to weather …

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Evaluation Of Fish Population Estimation By Removal Sampling In King's Creek, Kansas, Hope Phillips Jan 2000

Evaluation Of Fish Population Estimation By Removal Sampling In King's Creek, Kansas, Hope Phillips

Honors Theses, 1963-2015

Population estimation is an integral part of fisheries science. Using electrofishing to conduct a multiple-pass depletion-removal method to estimate population parameters is common. However, this method is time consuming and labor intensive. Recent research suggests that, under certain circumstances, reliable population estimates from a single-pass electrofishing event can be obtained. The objectives of this study were 1) to determine if fish density and catch-per-unit-effort (CPUE) from a single-pass electrofishing event is correlated with population density estimates obtained from the depletion-removal method and 2) to describe relationships among habitat variables and probability of capture from electrofishing. The study was conducted on …

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Ego-1 Is Related To Rna-Directed Rna Polymerase And Functions In Germ-Line Development And Rna Interference In C. Elegans, Anne Smardon, Jill M. Spoerke, Steven C. Stacey, Marcia E. Klein, Nancy Mackin, Eleanor M. Maine Jan 2000

Ego-1 Is Related To Rna-Directed Rna Polymerase And Functions In Germ-Line Development And Rna Interference In C. Elegans, Anne Smardon, Jill M. Spoerke, Steven C. Stacey, Marcia E. Klein, Nancy Mackin, Eleanor M. Maine

Biology - All Scholarship

Background: Cell-fate determination requires that cells choose between alternative developmental pathways. For example, germ cells in the nematode worm Caenorhabditis elegans choose between mitotic and meiotic division, and between oogenesis and spermatogenesis. Germ-line mitosis depends on a somatic signal that is mediated by a Notch-type signaling pathway. The ego-1 gene was originally identified on the basis of genetic interactions with the receptor in this pathway and was also shown to be required for oogenesis. Here, we provide more insight into the role of ego-1 in germ-line development. Results: We have determined the ego-1 gene structure and the molecular basis of …

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Paradigm Shifts In Neural Induction, Scott F. Gilbert Jan 2000

Paradigm Shifts In Neural Induction, Scott F. Gilbert

Biology Faculty Works

The molecularization of developmental biology was originally seen as a challenge to the integrity of that discipline. However, important new insights from the analysis of gene expression soon transformed the field from one of experimental anatomy to one of developmental genetics. One of the main areas to be transformed from an anatomical to a molecular study was « primary embryonic induction ». The molecular analyses showed that some of the fundamental concepts concluded from the experimental embryological approach to primary embryonic induction were false. First, the neural fate of cells was not being induced. Rather, the epidermal fate was induced …

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Comparing Genotypic And Phenotypic Methods For Differentiating Strains Of Escherichia Coli Isolated From The Environment, Lateef Y. Saffore Jan 2000

Comparing Genotypic And Phenotypic Methods For Differentiating Strains Of Escherichia Coli Isolated From The Environment, Lateef Y. Saffore

Theses, Dissertations and Capstones

Escherichia coli is a Gram-negative, oxidase-negative, lactose fermenting rod belonging to the family Enterohacleriaceae. The enteric bacteria are common inhabitants of animal and human guts, but are not considered to be native to the environment. The presence of E. coli and similar enteric bacteria (coliforms) in surface water or groundwater implies the presence of fecal contamination from human or animal sources. E. coli has been used as an indicator of sewage-contaminated water since the 1930's and some strains are also causative agents of food or waterborne disease (Francy et al., 1993). The severity of disease can vary markedly depending …

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Cytotoxic Mab From Rheumatic Carditis Recognizes Heart Valves And Laminin, Jeffrey E. Galvin, Mark E. Hemric, Kent Ward, Madeleine W. Cunningham Jan 2000

Cytotoxic Mab From Rheumatic Carditis Recognizes Heart Valves And Laminin, Jeffrey E. Galvin, Mark E. Hemric, Kent Ward, Madeleine W. Cunningham

Faculty Publications and Presentations

Anti-streptococcal antibodies cross-reactive with N-acetyl-bD-glucosamine (GlcNAc) and myosin are present in the sera of patients with rheumatic fever (RF). However, their role in tissue injury is not clear. In this study, we show that anti-GlcNAc/anti-myosin mAb 3.B6 from a rheumatic carditis patient was cytotoxic for human endothelial cell lines and reacted with human valvular endothelium and underlying basement membrane. Reactivity of mAb 3.B6 with the valve was inhibited by human cardiac myosin > laminin > GlcNAc. The mAb 3.B6 epitopes were localized in fragments of human cardiac myosin, including heavy meromyosin (HMM), the S1 subfragment, and two light meromyosin (LMM) peptides containing …

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Blue-Throated Hummingbird Song: A Pinnacle Of Nonoscine Vocalizations, Millicent S. Ficken, Kathryn M. Rusch, Sandra J. Taylor, Donald R. Powers Jan 2000

Blue-Throated Hummingbird Song: A Pinnacle Of Nonoscine Vocalizations, Millicent S. Ficken, Kathryn M. Rusch, Sandra J. Taylor, Donald R. Powers

Faculty Publications - Department of Biological & Molecular Science

Little is known about the structure and function of hummingbird vocalizations. We studied the vocalizations of Blue-throated Hummingbirds (Lampornis clemenciae) at two sites in southeastern Arizona. Songs were produced by males and females. Male songs consisted of arrays of notes organized in clusters of ‘‘song units.’’ Within sites, all males shared the same song units. Individual differences occurred in some temporal aspects of song, and slight but consistent differences in note structure occurred between the two sites. The organization of units within songs was marked by rigid syntax, and long songs were produced by agglutination of units. Male …

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The Holliday Junction In An Inverted Repeat Dna Sequence: Sequence Effects On The Structure Of Four-Way Junctions, Brandt F. Eichman, Jeff Vargason, Blaine H.M. Mooers, P. Shing Ho Jan 2000

The Holliday Junction In An Inverted Repeat Dna Sequence: Sequence Effects On The Structure Of Four-Way Junctions, Brandt F. Eichman, Jeff Vargason, Blaine H.M. Mooers, P. Shing Ho

Faculty Publications - Department of Biological & Molecular Science

Holliday junctions are important structural intermediates in recombination, viral integration, and DNA repair. We present here the single-crystal structure of the inverted repeat sequence d(CCGGTACCGG) as a Holliday junction at the nominal resolution of 2.1 Å. Unlike the previous crystal structures, this DNA junction has B-DNA arms with all standard Watson–Crick base pairs; it therefore represents the intermediate proposed by Holliday as being involved in homologous recombination. The junction is in the stacked-X conformation, with two interconnected duplexes formed by coaxially stacked arms, and is crossed at an angle of 41.4° as a right-handed X. A sequence comparison with previous …

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The Extended And Eccentric E-Dna Structure Induced By Cytosine Methylation Or Bromination, Jeff Vargason, Brandt F. Eichman, P. Shing Ho Jan 2000

The Extended And Eccentric E-Dna Structure Induced By Cytosine Methylation Or Bromination, Jeff Vargason, Brandt F. Eichman, P. Shing Ho

Faculty Publications - Department of Biological & Molecular Science

Cytosine methylation or bromination of the DNA sequence d(GGCGCC)2 is shown here to induce a novel extended and eccentric double helix, which we call E-DNA. Like B-DNA, E-DNA has a long helical rise and bases perpendicular to the helix axis. However, the 3′-endo sugar conformation gives the characteristic deep major groove and shallow minor groove of A-DNA. Also, if allowed to crystallize for a period of time longer than that yielding E-DNA, the methylated sequence forms standard A-DNA, suggesting that E-DNA is a kinetically trapped intermediate in the transition to A-DNA. Thus, the structures presented here chart a crystallographic pathway …

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B2-Adrenergic Receptor Activates Extracellular Signal-Regulated Kinases (Erks) Via The Small G Protein Rap1 And The Serine/Threonine Kinase B-Raf, John M. Schmitt, Philip J. S. Stork Jan 2000

B2-Adrenergic Receptor Activates Extracellular Signal-Regulated Kinases (Erks) Via The Small G Protein Rap1 And The Serine/Threonine Kinase B-Raf, John M. Schmitt, Philip J. S. Stork

Faculty Publications - Department of Biological & Molecular Science

G protein-coupled receptors can induce cellular proliferation by stimulating the mitogen-activated protein (MAP) kinase cascade. Heterotrimeric G proteins are composed of both a and By subunits that can signal independently to diverse intracellular signaling pathways including those that activate MAP kinases. In this study, we examined the ability of isoproterenol, an agonist of the B2-adrenergic receptor (b2AR), to stimulate extracellular signal-regulated kinases (ERKs). Using HEK293 cells, which express endogenous b2AR, we show that isoproterenol stimulates ERKs via b2AR. This action of isoproterenol requires cAMP-dependent protein kinase and is insensitive to pertussis toxin, suggesting that Gas activation of cAMP-dependent protein kinase …

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Cd28 And The Tyrosine Kinase Lck Stimulate Mitogen-Activated Protein Kinase Activity In T Cells Via Inhibition Of The Small G Protein Rap1, Kendall D. Carey, Tara J. Dillon, John M. Schmitt, Allison M. Baird, Amy D. Holdorf, David B. Straus, Andrey S. Shaw, Philip J. S. Stork Jan 2000

Cd28 And The Tyrosine Kinase Lck Stimulate Mitogen-Activated Protein Kinase Activity In T Cells Via Inhibition Of The Small G Protein Rap1, Kendall D. Carey, Tara J. Dillon, John M. Schmitt, Allison M. Baird, Amy D. Holdorf, David B. Straus, Andrey S. Shaw, Philip J. S. Stork

Faculty Publications - Department of Biological & Molecular Science

Proliferation of T cells via activation of the T-cell receptor (TCR) requires concurrent engagement of accessory costimulatory molecules to achieve full activation. The best-studied costimulatory molecule, CD28, achieves these effects, in part, by augmenting signals from the TCR to the mitogen-activated protein (MAP) kinase cascade. We show here that TCR-mediated stimulation of MAP kinase extracellular-signal-regulated kinases (ERKs) is limited by activation of the Ras antagonist Rap1. CD28 increases ERK signaling by blocking Rap1 action. CD28 inhibits Rap1 activation because it selectively stimulates an extrinsic Rap1 GTPase activity. The ability of CD28 to stimulate Rap1 GTPase activity was dependent on the …

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Interface Between Titanium Miniplate/Screw And Human Calvaria, Kun Hwang, John M. Schmitt, Jeffrey O. Hollinger Jan 2000

Interface Between Titanium Miniplate/Screw And Human Calvaria, Kun Hwang, John M. Schmitt, Jeffrey O. Hollinger

Faculty Publications - Department of Biological & Molecular Science

This study demonstrates interfacial changes of titanium miniplate/screw with normal calvaria and nonvascularized calvarial bone graft ensued from craniectomy in a 53-year-old female. In 18 months after operation, a right parietal bone containing an L-shaped miniplate with screws, 5mm long and 2mm in external diameter, was harvested, fixed, and embedded in methylmetacrylate. Fifteen micrometer thick sections were made by an EXAKT cutting-grinding system (Exakt Company, Hamburg, Germany), and reviewed under the bright field light microscope. The mean of the bonecontacting surface ratio (BCSR) in six screwed bone was 64.1%; 69.3% in normal bone, and 60.4% in grafted bone. The trabecular …

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Does The Aquatic Salamander, Siren Intermedia, Respond To Chemical Cues From Prey?, Aaron M. Sullivan, Paul W. Frese, Alicia Mathis Jan 2000

Does The Aquatic Salamander, Siren Intermedia, Respond To Chemical Cues From Prey?, Aaron M. Sullivan, Paul W. Frese, Alicia Mathis

Faculty Publications - Department of Biological & Molecular Science

No abstract provided.

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Rana Areolata Circulosa (Northern Crawfish Frog) Vocalization, Paul W. Frese, Aaron M. Sullivan Jan 2000

Rana Areolata Circulosa (Northern Crawfish Frog) Vocalization, Paul W. Frese, Aaron M. Sullivan

Faculty Publications - Department of Biological & Molecular Science

No abstract provided.

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Binding Of Urate And Caffeine To Hemocyanin Of The Lobster Homarus Vulgaris (E.) As Studied By Isothermal Titration Calorimetry., Michael Menze, Nadja Hellmann, Heinz Decker, Manfred Grieshaber Jan 2000

Binding Of Urate And Caffeine To Hemocyanin Of The Lobster Homarus Vulgaris (E.) As Studied By Isothermal Titration Calorimetry., Michael Menze, Nadja Hellmann, Heinz Decker, Manfred Grieshaber

Faculty and Staff Scholarship

Hemocyanin serves as an oxygen carrier in the hemolymph of the European lobster Homarus Vulgaris. The oxygen binding behavior of the pigment is modulated by metabolic effectors such as lactate and urate. Urate and caffeine binding to 12-meric hemocyanin (H. Vulgaris) was studied using isothermal titration calorimetry (ITC). Binding isotherms were determined for fully oxygenated hemocyanin between pH 7.55 and 8.15. No pH dependence of the binding parameters could be found for either effector. Since the magnitude of the Bohr effect depends on the urate concentration, the absence of any pH dependence of urate and caffeine binding to oxygenated hemocyanin …

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The Mayfly Newsletter, Peter M. Grant Jan 2000

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

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Genes Classical And Genes Developmental: The Different Uses Of The Gene In Evolutionary Synthesis, Scott F. Gilbert Jan 2000

Genes Classical And Genes Developmental: The Different Uses Of The Gene In Evolutionary Synthesis, Scott F. Gilbert

Biology Faculty Works

Dobzhansky (1964) stated that “Nothing in biology makes sense except in the light of evolution,” and the function of the gene is no exception. The use of genes in population genetics and developmental genetics differs significantly. This is reflected in the roles that genes are postulated to play in evolution. In the Modern Synthesis of population genetics and evolution, genes become manifest by differences in alleles that are active in conferring differential reproductive success in adult individuals. The gene is thought to act as a particulate, atomic unit. In current syntheses of evolution and developmental genetics, important genes are manifest …

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Studies On The Role Creb As A Mediator Of Neurotrophin-3 Actions In Oligodendrocytes, Jamie Richard Johnson Jan 2000

Studies On The Role Creb As A Mediator Of Neurotrophin-3 Actions In Oligodendrocytes, Jamie Richard Johnson

Theses and Dissertations

In the central nervous system (CNS), oligodendrocytes (OLGs) are the cells responsible for producing the myelin membrane which allows for the saltatory conduction of neuronal impulses. We have previously shown that CREB (cAMP response element binding protein), a transcription factor that belongs to a large family bZip (basic leucine zipper) proteins, could be a mediator of neuronal signals that, coupled to different signal transduction pathways, may play different regulatory roles at specific stages of oligodendrocyte development. We have found before that, in committed OLGs, CREB activation by phosphorylation can be triggered by β-adrenergic stimulation and appears to play a role …

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Of Moths And Men: Theo Lang And The Persistence Of Richard Goldschmidt's Theory Of Homosexuality, 1916-1960, Michael Dietrich Jan 2000

Of Moths And Men: Theo Lang And The Persistence Of Richard Goldschmidt's Theory Of Homosexuality, 1916-1960, Michael Dietrich

Dartmouth Scholarship

Using an analogy between moths and men, in 1916, Richard Goldschmidt proposed that homosexuality was a case of genetic intersexuality. As he strove to create a unified theory of sex determination that would encompass animals ranging from moths to men, Goldschmidt's doubts grew concerning the association of homosexuality with intersexuality until, in 1931, he dropped homosexuality from his theory of intersexuality. Despite Goldschmidt's explicit rejection of his theory of homosexuality, Thee Lang, a researcher in the Genealogical-Demographic Department of the Institute for Psychiatric Research in Munich, revived it, maintained Goldschmidt's association with it, and argued on its behalf in publications …

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Detection Of Dna Of Nocturnally Periodic Brugia Malayi In Night And Day Blood Samples By A Polymerase Chain Reaction-Elisa-Based Method Using An Internal Control Dna, Peter U. Fischer, Tanianwati Supali, Heri Wibowo, Insa Bonow, Steven A. Williams Jan 2000

Detection Of Dna Of Nocturnally Periodic Brugia Malayi In Night And Day Blood Samples By A Polymerase Chain Reaction-Elisa-Based Method Using An Internal Control Dna, Peter U. Fischer, Tanianwati Supali, Heri Wibowo, Insa Bonow, Steven A. Williams

Biological Sciences: Faculty Publications

An internal control was used in a polymerase chain reaction (PCR)–ELISA–based technique to detect the Hha I repeat of the filarial parasite Brugia malayi. A single microfilaria added to 200 �l of blood was reliably detected. The assay was evaluated on field samples from persons living in an area endemic for Anopheles-transmitted, nocturnally periodic B. malayi in central Sulawesi, Indonesia. Examination of night blood of 138 individuals for the presence of microfilariae by filtration revealed 44 microfilaria carriers. All microfilaria carriers were also positive in the PCR-ELISA and, in addition, 14 more samples were proven to contain parasite DNA. The …

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Egg-Derived Proteases As A Block To Polyspermy In The Eggs Of The Freshwater Mussel, Dreissena Polymorpha, Alicia Kay Smith Wysong Jan 2000

Egg-Derived Proteases As A Block To Polyspermy In The Eggs Of The Freshwater Mussel, Dreissena Polymorpha, Alicia Kay Smith Wysong

Honors Capstones

No abstract provided.

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