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Identifying Sources Of Fecal Pollution In The Roanoke River, Roanoke County, Virginia, J. Brooks Crozier, Brian Clark, Holly Weber Oct 2002

Identifying Sources Of Fecal Pollution In The Roanoke River, Roanoke County, Virginia, J. Brooks Crozier, Brian Clark, Holly Weber

Virginia Journal of Science

Antibiotic Resistance Patterns (ARPs) of Enterococcus spp. were used as a phenotypic fingerprint to compare and categorize unknown-source isolates in an impaired segment of the Roanoke River, Roanoke County, Virginia. Antibiotic resistance analysis (ARA) of enterococci has been effectively used to differentiate among sources of fecal contamination in many geographic regions in the United States. Enterococcus spp. were used as a fecal indicator in a library consisting of 1,562 known-source isolates. Two-way analysis indicated that approximately 95% of the unknown-source isolates collected were of animal origin. A 3-way analysis indicated that 61% of the unknowns were of livestock origin while …


Asi1, A Gene Encoding A Novel Leucine Zipper Protein, Is Induced During Development Of The Macronucleus In Tetrahymena, Rupa A. Udani, Kathleen M. Karrer Oct 2002

Asi1, A Gene Encoding A Novel Leucine Zipper Protein, Is Induced During Development Of The Macronucleus In Tetrahymena, Rupa A. Udani, Kathleen M. Karrer

Biological Sciences Faculty Research and Publications

Sexual reproduction in the ciliate Tetrahymena follows a complex developmental program involving the sequential regulation of dozens of genes. Genes that are up-regulated during post-zygotic development in Tetrahymena were isolated by subtractive hybridization. Anlagen stage induced gene 1 (ASI1) encodes a 2.8 kb transcript that contains a single intron and is induced during macronuclear development. ASI1 is a single copy gene in both the micronucleus and the macronucleus. It encodes a 95 kDa conceptual protein with a leucine zipper near the amino terminus


Molecular Mechanisms Of Metabolic Arrest: Life In Limbo (Book Review), Bernard Rees Oct 2002

Molecular Mechanisms Of Metabolic Arrest: Life In Limbo (Book Review), Bernard Rees

Biological Sciences Faculty Publications

No abstract provided.


Histological Evaluation Of Cryopreserved Ovaries In Autologous Abdominal Transplant To NaïVe, Outbred, Cd1, Mice, Wentia Elissa Ford Oct 2002

Histological Evaluation Of Cryopreserved Ovaries In Autologous Abdominal Transplant To NaïVe, Outbred, Cd1, Mice, Wentia Elissa Ford

Biological Sciences Theses & Dissertations

This study showed that fresh and frozen-thawed mouse ovaries would maintain viable primordial or primary follicles post-autologous transplantation. Furthermore, with appropriate hormonal stimulation, these early-stage follicles were shown to develop into Graafian follicles containing a metaphase II antral ovum. Partially sliced ovaries were transplanted into the abdominal cavity of naive, outbred, CD1, white mice. Hormonal stimulation with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) one week after the autologous transplantation stimulated variable degrees of follicle and ovum maturation within the ovarian tissue. There were no major morphological differences observed between the four groups, three treatment and one …


Nearshore Physical Processes And Bio-Optical Properties In The New York Bight, G. C. Chang, T. D. Dickey, Oscar M. Schofield, A. D. Weidemann, E. Boss, W. S. Pegau, Mark A. Moline, Scott M. Glenn Sep 2002

Nearshore Physical Processes And Bio-Optical Properties In The New York Bight, G. C. Chang, T. D. Dickey, Oscar M. Schofield, A. D. Weidemann, E. Boss, W. S. Pegau, Mark A. Moline, Scott M. Glenn

Biological Sciences

Temporal and spatial variability of physical, biological, and optical properties on scales of minutes to months and meters to ~50 km are examined using an extensive data set collected on the New York Bight continental shelf during the Hyperspectral Coastal Ocean Dynamics Experiment. Measurements from a midshelf mooring and bottom tripod (~25 km offshore, 24 m water depth) and two nearshore profiling nodes (~5 km offshore, 15 m water depth) are utilized to quantify and correlate midshelf and nearshore variability. Towed shipboard undulating profilers and a high-frequency radar (CODAR) array provide complementary spatial data. We show that phytoplankton and dissolved …


Distinct Interactions Of Gtp, Utp, And Ctp With G(S) Proteins, Andreas Gille, Hui-Yu Liu, Stephen R. Sprang, Roland Seifert Sep 2002

Distinct Interactions Of Gtp, Utp, And Ctp With G(S) Proteins, Andreas Gille, Hui-Yu Liu, Stephen R. Sprang, Roland Seifert

Biological Sciences Faculty Publications

Early studies showed that in addition to GTP, the pyrimidine nucleotides UTP and CTP support activation of the adenylyl cyclase (AC)-stimulating G(s) protein. The aim of this study was to elucidate the mechanism by which UTP and CTP support G(s) activation. As models, we used S49 wild-type lymphoma cells, representing a physiologically relevant system in which the beta(2)-adrenoreceptor (beta(2)AR) couples to G(s), and Sf9 insect cell membranes expressing beta(2)AR-Galpha(s) fusion proteins. Fusion proteins provide a higher sensitivity for the analysis of beta(2)AR-G(s) coupling than native systems. Nucleoside 5'-triphosphates (NTPs) supported agonist-stimulated AC activity in the two systems and basal AC …


A Novel High-Cell-Density Protein Expression System Based On Ralstonia Eutropha, Sriram Srinivasan, Gavin C. Barnard, Tillman U. Gerngross Sep 2002

A Novel High-Cell-Density Protein Expression System Based On Ralstonia Eutropha, Sriram Srinivasan, Gavin C. Barnard, Tillman U. Gerngross

Dartmouth Scholarship

We describe the development of a novel protein expression system based on the industrial fermentation organism Ralstonia eutropha (formerly known as Alcaligenes eutrophus) NCIMB 40124. This new system over- comes some of the shortcomings of traditional Escherichia coli-based protein expression systems, particularly the propensity of such systems to form inclusion bodies during high-level expression. Using a proteomics approach, we identified promoters that can be induced by simple process parameters or medium compositions in high-density cell culture or shake flasks, respectively. By combining newly developed molecular biological tools with a high-cell-density fermentation process, we were able to produce high levels ( …


Biology Department Publications: 2002, Sam Lindgren Sep 2002

Biology Department Publications: 2002, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2002. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.


Insertion Of Proteins Into The Inner Membrane Of Mitochondria: The Role Of The Oxa1 Complex, Rosemary A. Stuart Sep 2002

Insertion Of Proteins Into The Inner Membrane Of Mitochondria: The Role Of The Oxa1 Complex, Rosemary A. Stuart

Biological Sciences Faculty Research and Publications

The inner mitochondrial membrane harbors a large number of proteins that display a wide range of topological arrangements. The majority of these proteins are encoded in the cell's nucleus, but a few polytopic proteins, all subunits of respiratory chain complexes are encoded by the mitochondrial genome. A number of distinct sorting mechanisms exist to direct these proteins into the mitochondrial inner membrane. One of these pathways involves the export of proteins from the matrix into the inner membrane and is used by both proteins synthesized within the mitochondria, as well as by a subset of nuclear encoded proteins. Prior to …


Review Of: Riverine Flood Plains: Present State And Future Trends, Klement Tockner, Jack Arthur Stanford Sep 2002

Review Of: Riverine Flood Plains: Present State And Future Trends, Klement Tockner, Jack Arthur Stanford

Biological Sciences Faculty Publications

Natural flood plains are among the most biologically productive and diverse ecosystems on earth. Globally, riverine flood plains cover > 2 x 10(6) km(2), however, they are among the most threatened ecosystems. Floodplain degradation is closely linked to the rapid decline in freshwater biodiversity; the main reasons for the latter being habitat alteration, flow and flood control, species invasion and pollution. In Europe and North America, up to 90% of flood plains are already 'cultivated' and therefore functionally extinct. In the developing world, the remaining natural flood plains are disappearing at an accelerating rate, primarily as a result of changing hydrology. …


Current Issues In Forensic Dna Profiling, Dan E. Krane Sep 2002

Current Issues In Forensic Dna Profiling, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.


Stephen J. Gould, Scott F. Gilbert Sep 2002

Stephen J. Gould, Scott F. Gilbert

Biology Faculty Works

No abstract provided.


Eye Suppression, A Novel Function Of Teashirt, Requires Wingless Signaling, Amit Singh, Madhuri Kango-Singh, Y. Henry Sun Sep 2002

Eye Suppression, A Novel Function Of Teashirt, Requires Wingless Signaling, Amit Singh, Madhuri Kango-Singh, Y. Henry Sun

Biology Faculty Publications

Teashirt (tsh) encodes a Drosophila zinc-finger protein. Misexpression of tsh has been shown to induce ectopic eye formation in the antenna. We report that tsh can suppress eye development. This novel function of tsh is due to the induction of homothorax (hth), a known repressor of eye development, and requires Wingless (WG) signaling. Interestingly, tsh has different functions in the dorsal and ventral eye, suppressing eye development close to the ventral margin, while promoting eye development near the dorsal margin. It affects both growth of eye disc and retinal cell differentiation.


Reduced Reproductive Effort In Male Field Crickets Infested With Parasitoid Fly Larvae, Gita R. Kolluru, Marlene Zuk, Mark A. Chappell Sep 2002

Reduced Reproductive Effort In Male Field Crickets Infested With Parasitoid Fly Larvae, Gita R. Kolluru, Marlene Zuk, Mark A. Chappell

Biological Sciences

Some populations of the field cricket Teleogryllus oceanicus are parasitized by the phonotactic fly Ormia ochracea. Flies locate crickets by their song and deposit larvae onto them. The larvae develop inside the cricket for 1 week before killing the host upon emergence. The reproductive compensation hypothesis predicts that parasitized crickets should increase their reproductive effort during the initial stages of infestation to offset the loss of fitness resulting from their shortened life span. An alternative hypothesis predicts that parasitized crickets will decrease reproduction, either because they are unable to reproduce or because selection acting on the parasitoid favors decreased …


The Tadpole Of Proceratophrys Avelinoi (Anura: Leptodactylidae), Rafael O. De Sá, José A. Langone Sep 2002

The Tadpole Of Proceratophrys Avelinoi (Anura: Leptodactylidae), Rafael O. De Sá, José A. Langone

Biology Faculty Publications

The genus Proceratoprhrys is poorly known. It consists of 14 currently recognized species (Frost, 2000) of medium-sized frogs distributed from northeastern Argentina and Paraguay to southeast Amazonia (Rondonia State), eastern and southern Brazil. Proceratophrys avelinoi was described from Misiones, Argentina (Mercadal de Barrio and Barrio, 1993). The larval stage of this species is unknown. Herein, we describe the tadpole and the characteristics of the internal oral anatomy of P avelinoi using scanning electron micros- copy (SEM).


Riparian Vegetation Of The Lower Rio Grande, Robert I. Lonard, Frank W. Judd Sep 2002

Riparian Vegetation Of The Lower Rio Grande, Robert I. Lonard, Frank W. Judd

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The riparian vegetation of the lower reach of the Rio Grande was studied at 7 locations using 3 line intercepts at each location. There were no trees at the mouth of the river and the vegetation was similar to that found along the Laguna Madre shore of barrier islands. Mesquite (Prosopis glandulosa) was the dominant tree near the coast and in the western section of the river near Falcon Dam. Sugar hackberry (Celtis laevigata) was the dominant tree species at all other sites except at Santa Ana National Wildlife Refuge, where cedar elm (Ulmus crassifolia) and anacua (Ehretia anacua) were …


Cosolvent-Induced Transformation Of A Death Domain Tertiary Structure, Tsan Xiao, Kevin H. Gardner, Stephen R. Sprang Aug 2002

Cosolvent-Induced Transformation Of A Death Domain Tertiary Structure, Tsan Xiao, Kevin H. Gardner, Stephen R. Sprang

Biological Sciences Faculty Publications

The death domain (DD) of the protein kinase Pelle adopts a six-helix bundle fold in the crystal structure of the complex with its dimerization partner, Tube-DD. However, in crystals obtained from a solution of 45% 2-methyl-2,4-pentanediol (MPD), the C-terminal half of Pelle-DD folds into a single helix, and the N-terminal half of the molecule is disordered. The helical segment forms an antiparallel dimer with the corresponding helix of a symmetry-related molecule, and together they form extensive lattice interactions similar in number, composition, and buried surface to those in the six-helix bundle of the native fold. Secondary structure analysis by heteronuclear …


Ua1b1/7 Groundbreaking For Complex For Engineering & Biological Sciences, Wku Office Of Event Planning Aug 2002

Ua1b1/7 Groundbreaking For Complex For Engineering & Biological Sciences, Wku Office Of Event Planning

WKU Administration Documents

Program for the Complex for Engineering & Biological Science groundbreaking ceremony.


Ua1b1/7 Groundbreaking Complex For Engineering & Biological Sciences, Western Kentucky University Aug 2002

Ua1b1/7 Groundbreaking Complex For Engineering & Biological Sciences, Western Kentucky University

WKU Administration Documents

Invitation to the Complex for Engineering & Biological Sciences groundbreaking.


The N-Acetyltransferase Rimj Responds To Environmental Stimuli To Repress Pap Fimbrial Transcription In Escherichia Coli, Christine A. White-Ziegler, Alia M. Black, Stacie H. Eliades, Sarah Young, Kimberly Porter Aug 2002

The N-Acetyltransferase Rimj Responds To Environmental Stimuli To Repress Pap Fimbrial Transcription In Escherichia Coli, Christine A. White-Ziegler, Alia M. Black, Stacie H. Eliades, Sarah Young, Kimberly Porter

Biological Sciences: Faculty Publications

In uropathogenic Escherichia coli, P pili (Pap) facilitate binding to host epithelial cells and subsequent colonization. Whereas P pili can be produced at 37°C, the expression of these fimbriae is suppressed at 23°C. Previously, insertion mutations in rimJ, a gene encoding the N-terminal acetyltransferase of ribosomal protein S5, were shown to disrupt this thermoregulatory response, allowing papBA transcription at low temperature. In this study, we created an in-frame deletion of rimJ. This deletion relieved the repressive effects not only of low temperature but also of rich (Luria-Bertani [LB]) medium and glucose on papBA transcription, indicating that RimJ modulates papBA transcription …


The Heat-Shock Response: Its Variation, Regulation And Ecological Importance In Intertidal Gastropods (Genus Tegula), Lars Tomanek Aug 2002

The Heat-Shock Response: Its Variation, Regulation And Ecological Importance In Intertidal Gastropods (Genus Tegula), Lars Tomanek

Biological Sciences

The enhanced synthesis of heat-shock proteins (hsps), called the heat-shock (or stress) response, is activated when environmental stress denatures proteins. Hsp synthesis is activated at the upper temperatures of an organism's thermal range and is therefore thought to be critical for enhancing thermal tolerance limits in ectothermic animals. Here I show that the two temperate sister species T. brunnea and T. montereyi that occupy the subtidal and low-intertidal zone differ from the low- to mid-intertidal T. funebralis (and the subtropical mid-intertidal T. rugosa) in (i) heat tolerance, (ii) the onset temperature of their main hsp, hsp70 (70 kDa), (iii) …


Discovering Lactic Acid Bacteria By Genomics, Todd Klaenhammer, Eric Altermann, Fabrizio Arigoni, Alexander Bolotin, Fred Breidt, Jeffrey Broadbent, Raul J. Cano, Stephane Chaillou, Josef Deutscher, Mike Gasson, Maarten Van De Guchte, Jean Guzzo, Axel Hartke, Trevor Hawkins, Pascal Hols, Robert Hutkins, Michiel Kleerebezem, Jan Kok, Oscar Kuipers, Mark Lubbers, Emmanuelle Maguin, Larry Mckay, David Mills, Arjen Nauta, Ross Overbeek, Herman Pel, David Pridmore, Milton Saier, Douwe Van Sinderen, Alexei Sorokin, James Steele, Daniel O'Sullivan, Willem De Vos, Bart Weimer, Monique Zagorec, Roland Siezen Aug 2002

Discovering Lactic Acid Bacteria By Genomics, Todd Klaenhammer, Eric Altermann, Fabrizio Arigoni, Alexander Bolotin, Fred Breidt, Jeffrey Broadbent, Raul J. Cano, Stephane Chaillou, Josef Deutscher, Mike Gasson, Maarten Van De Guchte, Jean Guzzo, Axel Hartke, Trevor Hawkins, Pascal Hols, Robert Hutkins, Michiel Kleerebezem, Jan Kok, Oscar Kuipers, Mark Lubbers, Emmanuelle Maguin, Larry Mckay, David Mills, Arjen Nauta, Ross Overbeek, Herman Pel, David Pridmore, Milton Saier, Douwe Van Sinderen, Alexei Sorokin, James Steele, Daniel O'Sullivan, Willem De Vos, Bart Weimer, Monique Zagorec, Roland Siezen

Biological Sciences

This review summarizes a collection of lactic acid bacteria that are now undergoing genomic sequencing and analysis. Summaries are presented on twenty different species, with each overview discussing the organisms fundamental and practical significance, environmental habitat, and its role in fermentation, bioprocessing, or probiotics. For those projects where genome sequence data were available by March 2002, summaries include a listing of key statistics and interesting genomic features. These efforts will revolutionize our molecular view of Gram-positive bacteria, as up to 15 genomes from the low GC content lactic acid bacteria are expected to be available in the public domain by …


A Mitochondrial Dna Phylogeny Of The Leptodactylus Fuscus Species-Complex (Leptodactylidae: Anura, Arley Camargo Aug 2002

A Mitochondrial Dna Phylogeny Of The Leptodactylus Fuscus Species-Complex (Leptodactylidae: Anura, Arley Camargo

Master's Theses

Leptodactylus fuscus is a broadly distributed frog species in the Neotropical region. Previous allozymic evidence supported the hypothesis of L. fuscus as a composite of several species. However, morphological and advertisement call data supported the hypothesis of a single-species taxon. These competing hypotheses were evaluated using cladistic analyses of mitochondrial DNA sequences. Fragments of 12S rRNA, 16S rRNA, and ND1 protein-coding genes from 17 populations were sequenced. Phylogenetic analyses were performed using: maximum parsimony, maximum likelihood, and Bayesian analysis. Data sets were analyzed separately and combined. Analyses of the combined data set show four exclusive clades of L. fuscus: …


Competition For Pollination Between An Invasive Species (Purple Loosestrife) And A Native Congener, Beverly J. Brown, Randall J. Mitchell, Shirley A. Graham Aug 2002

Competition For Pollination Between An Invasive Species (Purple Loosestrife) And A Native Congener, Beverly J. Brown, Randall J. Mitchell, Shirley A. Graham

Biology Faculty Research

Invasive species are frequently regarded as Superlative competitors that can vegetatively crowd Out natives, but little is known about whether invasives call compete for pollination services with native plants. We hypothesized that, when the showy invasive species Lythrum salicaria (purple loosestrife) was present, pollinator visitation and seed set would be reduced in a native congener, L. alatum (winged loosestrife). To test this hypothesis, we constructed mixed and monospecific plots of the two species. Over two years of study, we found that L. salicaria significantly reduced both pollinator visitation and seed set in L. alatum. Furthermore, pollinators moved frequently between the …


Effects Of Ethanol On The Toxicity And Metabolism Of Acetonitrile And Acrylonitrile, Nancy Ochoa-Garza Aug 2002

Effects Of Ethanol On The Toxicity And Metabolism Of Acetonitrile And Acrylonitrile, Nancy Ochoa-Garza

Theses and Dissertations - UTB/UTPA

This study examined the effects of ethanol on the toxicities and metabolism of two aliphatic nitriles: acetonitrile and acrylonitrile (one unsaturated and one saturated aliphatic nitrile, respectively) in male Sprague-Dawley rats at four different time intervals. Toxicity signs were apparent in all animals given the nitrile dosage, but those treated with ethanol dosages showed signs of toxicity earlier than those treated with glucose dosages. Both of the nitrites were metabolized to cyanide and thiocyanate. The concentration levels of each metabolite was apparent at 2 and 3 g/kg ethanol dosage for acetonitrile and 3 and 4.5 g/kg ethanol dosage for acrylonitrile …


From Development To Evolution: The Re-Establishment Of The "Alexander Kowalevsky Medal", A. T. Mikhailov, Scott F. Gilbert Aug 2002

From Development To Evolution: The Re-Establishment Of The "Alexander Kowalevsky Medal", A. T. Mikhailov, Scott F. Gilbert

Biology Faculty Works

The Saint Petersburg Society of Naturalists has reinstated the Alexander O. Kowalevsky Medal. This article announces the winners of the first medals and briefly reviews the achievements of A.O. Kowalevsky,the Russian comparative embryologist whose studies on amphioxus, tunicates and germ layer homologies pioneered evolutionary embryology and confirmed the evolutionary continuity between invertebrates and vertebrates. In re-establishing this international award, the Society is pleased to recognize both the present awardees and the memory of Kowalevsky, whose work pointed to that we now call evolutionary developmental biology.


The Biophysical Limitations In Physiological Transport And Exchange In Plants Grown In Microgravity, D. M. Porterfield Jul 2002

The Biophysical Limitations In Physiological Transport And Exchange In Plants Grown In Microgravity, D. M. Porterfield

Biological Sciences Faculty Research & Creative Works

This paper describes how changes in the physical behavior of fluids and gases in microgravity can limit the physiological transport and exchange in higher plants. These types of effects are termed indirect effects of microgravity because they are not due to gravity interacting with the mass of the plant body itself. The impact of limiting gravity-dependent transport phenomena has been analyzed by the use of mathematical modeling to simulate and compare biophysical transport in the 1g and spaceflight environments. These data clearly show that the microgravity environment induces significant limitations on basic physiological and biochemical processes within the aerial and …


Simple, Compact Source For Low-Temperature Air Plasmas, D. P. Sheehan, J. Lawson, M. Sosa, Richard A. Long Jul 2002

Simple, Compact Source For Low-Temperature Air Plasmas, D. P. Sheehan, J. Lawson, M. Sosa, Richard A. Long

Faculty Publications

A simple, compact source of low-temperature, spatially and temporally uniform air plasma using a Telsa induction coil driver is described. The low-power ionization discharge plasma is localized (2 cm X 0.5 cm X 0.1 cm) and essentially free of arc channels. A Teflon coated rolling cylindrical electrode and dielectric coated ground plate are essential to the source’s operation and allow flat test samples to be readily exposed to the plasma. The plasma is a copious source of ozone and nitrogen oxides. Its effects on various microbes are discussed.


Tolerance, Performance, And Aerobic Metabolism Of Redband Trout (Oncorhynchus Mykiss Ssp.) During Acute Exposure To Elevated Temperature, Karen Reed Lizars Jul 2002

Tolerance, Performance, And Aerobic Metabolism Of Redband Trout (Oncorhynchus Mykiss Ssp.) During Acute Exposure To Elevated Temperature, Karen Reed Lizars

Dissertations and Theses

The objective of this thesis was to determine whether the redband trout (Oncorhynchus mykiss ssp.) of Oregon's inland desert basins are negatively affected by the high peak temperatures (> 20 °C) often observed in their native streams. Small (40 - 140 g) redband trout from three streams (Bridge, Rock, and Twelve-mile creeks) were collected and tested on-site for aspects of thermal physiology, including: critical swimming velocity (Ucrit), maximum metabolic rate (MO2), metabolic power, and total cost of transport (TCT) at 24 °C; routine MO2 at 14 to 2: 28 °C: and critical thermal maximum (CTM). At …


Genetic Differentiation Of Rare And Common Varieties Of Eriogonum Shockleyi (Polygonaceae) In Idaho Using Issr Variability, James F. Smith Jul 2002

Genetic Differentiation Of Rare And Common Varieties Of Eriogonum Shockleyi (Polygonaceae) In Idaho Using Issr Variability, James F. Smith

Biology Faculty Publications and Presentations

Idaho populations of Eriogonum shockleyi are divided taxonomically into 2 varieties: E. shockleyi var. packardae , which is endemic to Idaho, and the typical variety, which is widespread in the western United States. Recent morphological investigations of E. shockleyi in Idaho have identified potentially reliable morphological characters for field identification of the subspecific taxa. This paper investigates the genetic basis for the separation of the 2 varieties of E. shockleyi using inter simple-sequence repeats (ISSR) markers. Although we found some morphological differences between the populations that correlated with the 2 varieties, we identified no molecular markers in this study to …