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Overcoming Barriers To Natural Orifice Translumenal Endoscopic Surgery (Notes), Steve J. Schomisch

ETD Archive

Natural Orifice Translumenal Endoscopic Surgery (NOTES) avoids skin incisions by accessing the abdominal cavity through natural orifices. Benefits include less pain, fewer complications and no scars. The aims of this study were to evaluate safety and efficacy of access techniques for natural orifice surgery, to investigate safety and efficacy of closure methodologies and to compare the inflammatory response induced by NOTES with that following conventional surgery. Access techniques were evaluated for safety and efficacy by measuring resultant injury and time required to access the peritoneal cavity in an acute porcine model. Four different gastrotomy closure modalities were evaluated for safety …

Myf5 Does Not Induce Apoptosis In Skeletal Myoblasts But Is Regulated By Oncogenic Ras Expression, Alexander Phillip Talarico

ETD Archive

Skeletal myogenesis is initiated by a family of muscle regulatory factors (MRFs) composed of the transcription factors MyoD, Myf5, MRF4, and myogenin. Of these, MyoD was the first discovered, is the most studied, and plays a well-characterized role in skeletal myoblast determination and differentiation. Our laboratory has also discovered that MyoD plays an important role in the apoptotic process that occurs in a subpopulation of myoblasts induced to differentiate. Expression of oncogenic Ras is known to inhibit myoblast differentiation and to decrease the expression of MyoD mRNA and protein. Our laboratory was the first to report that expression of oncogenic …

Cloning And Expression Of Aquaporin In The Antennal Gland Of Crayfish, Procambarus Clarkii, Yang Gao

Browse all Theses and Dissertations

Aquaporins (AQPs) are responsible for transferring water and other small molecules across the cell membrane. Relatively little research has been conducted on AQPs in crustaceans. To study the AQPs in crayfish, I hypothesis that 1) AQP is expressed in the antennal gland (kidney analogue) of crayfish; 2) crayfish AQP will show greater expression in tissues (gill and antennal gland) associated with volume regulation; 3) AQP mRNA expression in antennal gland and gill will increase in premolt and decrease in postmolt compared with intermolt level. RT-PCR and Real-time PCR assay were applied to test the hypothesis. A partial AQP gene was …

Functional Morphology And Fluid Interactions During Early Development Of The Scyphomedusa Aurelia Aurita, K. E. Feitl, A. F. Millett, Sean Colin, John O. Dabiri, John H. Costello

Arts & Sciences Faculty Publications

Scyphomedusae undergo a predictable ontogenetic transition from a conserved, universal larval form to a diverse array of adult morphologies. This transition entails a change in bell morphology from a highly discontinuous ephyral form, with deep clefts separating eight discrete lappets, to a continuous solid umbrella-like adult form. We used a combination of kinematic, modeling, and flow visualization techniques to examine the function of the medusan bell throughout the developmental changes of the scyphomedusa Aurelia aurita. We found that flow around swimming ephyrae and their lappets was relatively viscous (1 < Re < 10) and, as a result, ephyral lappets were surrounded by thick, overlapping boundary layers that occluded flow through the gaps between lappets. As medusae grew, their fluid environment became increasingly influenced by inertial forces (10 < Re < 10,000) and, simultaneously, clefts between the lappets were replaced by organic tissue. Hence, although the bell undergoes a structural transition from discontinuous (lappets with gaps) to continuous (solid bell) surfaces during development, all developmental stages maintain functionally continuous paddling surfaces. This developmental pattern enables ephyrae to efficiently allocate tissue to bell diameter increase via lappet growth, while minimizing tissue allocation to inter-lappet spaces that …

Caspase Cleavage Of The Amyloid Precursor Protein Is Prevented After Overexpression Of Bcl-2 In A Triple Transgenic Mouse Model Of Alzheimer’S Disease, Debra K. Kumasaka, Veronica Galvan, Elizabeth Head, Troy T. Rohn

Biology Faculty Publications and Presentations

A recent study demonstrated the lack of beta-amyloid (Aβ) plaque formation and accumulation of the amyloid precursor protein (APP) in a triple transgenic mouse model of Alzheimer’s disease (3xTg-AD) following overexpression of the anti-apoptotic protein, Bcl-2 (Rohn et al., J. Neurosci. 28: 3051-9, 2008). The supposition from that study was the accumulation of APP resulted from a decrease in caspase-mediated processing of APP. To determine a direct role for the caspase-cleavage of APP in 3xTg-AD mice, we designed a site-directed caspasecleavage antibody to APP and demonstrated it is a specific marker for caspase-cleaved APP. Application of this antibody revealed neuronal …

Afforestation Alters The Composition Of Functional Genes In Soil And Biogeochemical Processes In South American Grasslands, Sean T. Berthrong, Christopher W. Schadt, Gervasio Piñeiro, Robert B. Jackson

Scholarship and Professional Work - LAS

Soil microbes are highly diverse and control most soil biogeochemical reactions. We examined how microbial functional genes and biogeochemical pools responded to the altered chemical inputs accompanying land use change. We examined paired native grasslands and adjacent Eucalyptus plantations (previously grassland) in Uruguay, a region that lacked forests before European settlement. Along with measurements of soil carbon, nitrogen, and bacterial diversity, we analyzed functional genes using the GeoChip 2.0 microarray, which simultaneously quantified several thousand genes involved in soil carbon and nitrogen cycling. Plantations and grassland differed significantly in functional gene profiles, bacterial diversity, and biogeochemical pool sizes. Most grassland …

A Global Meta-Analysis Of Soil Exchangeable Cations, Ph, Carbon, And Nitrogen With Afforestation, Sean T. Berthrong, Esteban G. Jobbagy, Robert B. Jackson

Scholarship and Professional Work - LAS

Afforestation, the conversion of non-forested lands to forest plantations, can sequester atmospheric carbon dioxide, but the rapid growth and harvesting of biomass may deplete nutrients and degrade soils if managed improperly. The goal of this study is to evaluate how afforestation affects mineral soil quality, including pH, sodium, exchangeable cations, organic carbon, and nitrogen, and to examine the magnitude of these changes regionally where afforestation rates are high. We also examine potential mechanisms to reduce the impacts of afforestation on soils and to maintain long-term productivity.

Across diverse plantation types (153 sites) to a depth of 30 cm of mineral …

The Mechanism Of Expansion And The Volatility It Created In Three Pheromone Gene Clusters In The Mouse (Mus Musculus) Genome, Robert C. Karn, Christina M. Laukaitis

Scholarship and Professional Work - LAS

Three families of proteinaceous pheromones have been described in the house mouse: androgen-binding proteins (ABPs), exocrine gland–secreting peptides (ESPs), and major urinary proteins (MUPs), each of which is thought to communicate different information. All three are encoded by large gene clusters in different regions of the mouse genome, clusters that have expanded dramatically during mouse evolutionary history. We report copy number variation among the most recently duplicated Abp genes, which suggests substantial volatility in this gene region. It appears that groups of these genes behave as low copy repeats (LCRs), duplicating as relatively large blocks of genes by nonallelic homologous …

Wolf Spiders Of The Pacific Region: The Genus Zoica (Araneae, Lycosidae), Volker W. Framenau, James W. Berry, Joseph A. Beatty

Scholarship and Professional Work - LAS

The wolf spider genus Zoica Simon 1898 is currently known only from the Indo-Australasian region, including India in the west to northern Western Australia and Papua New Guinea in the east. Here we extend the known distribution of the genus into the Pacific region by describing two new species, Z. carolinensis new species from the Caroline Islands, Federated States of Micronesia, and Z. pacifica new species from the Republic of the Marshall Islands.

Assessment Of River Herring And Striped Bass In The Connecticut River: Abundance, Population Structure, And Predator/Prey Interactions, Justin P. Davis, Eric T. Schultz, Jason Vokoun

EEB Articles

Populations of anadromous alewife Alosa pseudoharengus and blueback herring A. aestivalis, collectively referred to as river herring, have declined in the Connecticut River. An explanatory hypothesis for these declines is that predation pressures have increased as a result of recent increases in abundance of sympatric striped bass Morone saxatilis. We sampled river herring and striped bass from the stretch of the Connecticut River between Wethersfield, CT and Holyoke, MA during the vernal migration seasons of 2005-2008. The objectives of the sampling program were to assess abundance, temporal/spatial distribution, and population structure of both river herring and striped bass, …

Tthe Immature Stages And Natural History Of Antirrhea Adoptiva Porphyrosticta (Watkins, 1928) In Eastern Ecuador (Lepidoptera, Nymphalidae, Morphinae), Harold F. Greeney, Philip J. Devries, Carla M. Penz, Rafael B. Granizo-T, Heidi Connahs, John O. Stireman Iii, Thomas R. Walla, Lee A. Dyer

Biological Sciences Faculty Publications

Here we describe the immature stages and ecological associations of Antirrhea adoptiva porphyrosticta Watkins, 1928 (Lepidoptera:Nymphalidae:Morphinae). The cloud forest bamboo, Chusquea scandens Kunth (Bambusoidea: Poaceae), serves as the larval food plant for this butterfly in eastern Ecuador, the first hostplant record for Antirrhea outside the family Arecaceae. The larvae of A. adoptiva porphyrosticta are superficially similar to those of other Antirrhea species. We also provide observations on adult and larval behavior. Caterpillars of this butterfly species are parasitized by tachinid flies, as well as by Ichneumonidae and a newly described braconid wasp.

Viability Of Subitaneous Eggs Of The Copepod, Acartia Tonsa (Dana), Following Exposure To Various Cryoprotectants And Hypersaline Water, Cortney L. Ohsa, Andrew L. Rhyne, Erik Stenn

Arts & Sciences Faculty Publications

Subitaneous eggs were obtained from monocultures of the calanoid copepod Acartia tonsa (Dana), Gulf of Mexico strain. Eggs were exposed to methanol, ethylene glycol, propylene glycol, glycerine, and DMSO at 0.0, 0.1, 0.5, 1.0, 2.0, and 5.0 M and hypersaline water at 50, 75, 100, 150, and 200 g/L. Treatments were evaluated after 10 and 20 min of exposure and at 4 and 26 °C. Viability (percent hatched) was determined after 24 h of incubation in 35 g/L saltwater at 26 °C.

Methanol, ethylene glycol, and glycerine had high viability up to 2M, and all experienced large decreases at 5M …

Seal Bounties In Maine And Massachusetts, 1888 To 1962, Barbarai Lelli, David Harris Ph.D, Aboueissa Abouel-Makarim

Faculty Publications

Maine and Massachusetts paid bounties on seals during the 19th and 20th centuries. To determine the number of seals killed for bounty, we examined historical records of bounty claims, and used geographic information systems and multiple linear regression to find predictors of places where large numbers of bounties were paid. We found records of 24,831 bounties paid in Maine (1891-1945) and 15,690 in Massachusetts (1888-1962), Considering possible fraud, missing data, and seals struck and lost, this suggests that 72,284 to 135,498 seals were killed in the bounty hunt, probably enough to account for regional declines in seal populations. Larger numbers …

Erk Activation Requires Cam Kinases In Mcf-7 Breast Cancer Cells, John M. Schmitt, Ellen Abell

Faculty Publications - Department of Biological & Molecular Science

Abstract A key signaling pathway involved in regulating cell growth and proliferation throughout the body is the ERK signaling pathway. ERK is activated via numerous pathways including intracellular calcium release downstream of G-Protein Coupled Receptors (GPCRs). Carbachol, a GPCR-agonist, both increases intracellular calcium and ERK activation in MCF-7 cells. ERK activation and control of cell growth may act through the transcription factor Elk-1. Our goal was to elucidate the specific proteins and kinases upstream of ERK in MCF-7 cells treated with carbachol. Secondly, we wanted to investigate the potential involvement of Elk-1 downstream of ERK. Carbachol treatment of MCF-7 cells …

Cam Kinase Kinase Control Of Prostate Cancer Cell Survival, John M. Schmitt, Brendon Hart

Faculty Publications - Department of Biological & Molecular Science

Abstract AKT has been implicated in promoting cell survival within certain cells. Current research has shown that hormones that increase the concentration of intracellular calcium can activate AKT that in turn leads to cancer cell survival. Interestingly, LNCaP cells express the M3-subtype of GPCR's that may couple to increases in intracellular calcium and activation of the Ca2+/Calmodulin-dependent Protein Kinases (CaM Ks). Specifically CaM Kinase Kinase (CaM KK) phosphorylates its direct substrates CaM Kinase I, CaM Kinase IV, and AKT. AKT promotes cell survival through phosphorylation of its target BAD that prevents caspase activation. Our goals were to examine the mechanism …

Primary Culture Of Isolated Cardiac Myocytes From The Bullfrog, Rana Catesbeiana, Jeff Duerr, Silvianne Luft

Faculty Publications - Department of Biological & Molecular Science

Comparative vertebrate studies into physiological responses to adverse conditions can yield great insight into the design of robust systems and provide fundamental knowledge of stress adaptations. The classically studied cellular stress response is the mitogen-activated protein kinase (MAPK) pathway. Prior investigations in our laboratory indicated a differential response of MAPK family activation to progressive dehydration in cardiac tissue from two species of anuran amphibians: Rana catesbeiana and Bufo marinus. Western blot analysis indicated that pERK increased by three-fold in Bufo and 2-fold in Rana. pJNK levels remained unchanged in Bufo whereas in Rana increased by 50%. pp38 did not exhibit …

Element Stoichiometry Of Ochromonas Danica As A Function Of Growth Rate And Temperature, Savannah Simonds

Biology Theses - Archive

The microbial loop stresses the importance of flagellated protozoa as predators of bacteria and regenerators of nutrients, the outcome of which affects higher trophic levels. The element stoichiometry of flagellates has only been examined while feeding upon bacteria or phytoplankton. In this study, the element stoichiometry of the protozoan flagellate, Ochromonas danica, was determined while growing osmotrophically thus eliminating the potential errors due to element contributions of prey in food vacuoles. The element and nucleic acid content of the flagellate were examined under a range of environmentally relevant temperatures and growth rates using chemostat cultures. Growth rate was the driving …

Diversity And Community Organization In The Periphyton Of Recirculating Streams Channels Are Defined By Current Velocity And Nutrient Supply, Chad A. Larson

Biology Dissertations - Archive

In a series of experiments taking place in recirculating artificial stream channels, periphyton communities growing on ceramic tiles were subjected to variations in current velocity and nutrient supply. The first experiment used stream water and manipulated current velocity, subjecting periphyton communities to either high or low current velocities (30 and 10 cm * sec-1 respectively), while in experiments 2-4, modified WC media was used and periphyton communities subjected to different nutrient (low and high) and current regimes (low, high, and variable flow). Each experimental run lasted 35 days. In all experiments, greater species richness of periphyton communities was observed in …

Natural Hybridization And Speciation In Toads Of The Anaxyrus Americanus Group., Brian E. Fontenot

Biology Dissertations - Archive

The goal of speciation research is to elucidate the processes that lead to the formation of new species. Examining natural hybridization and the evolution of reproductive isolating barriers provides an opportunity to understand the dynamic processes that occur before, during, and after speciation events. Here, I use true toads of the Anaxyrus americanus group to examine the effects of natural hybridization on (1) the evolution of reproductive isolation using previously published laboratory hybridization data on multiple species within the family Bufonidae, (2) morphological variation using morphometric analysis of known Anaxyrus americanus group hybrids, and (3) molecular variation and genetic population …

Evolutionary Morphology Of The Speckled Rattlesnakes (Viperidae: Crotalus Mitchellii Group), Jesse Malcolm Meik

Biology Dissertations - Archive

I examined various facets of morphological variation and evolution in the speckled rattlesnake (Crotalus mitchellii) species group. I performed three separate, but related, studies pertaining to the following topics: body size of putative ancestral source species and patterns of snake occupancy on islands in the Baja California region, the evolution and prevalence of gigantism and dwarfism among insular populations of speckled rattlesnakes, and the climatic correlates of scalation patterns in speckled rattlesnakes.I examined the influence of body size of mainland source species on insular distribution patterns by using a species index that weights isolation of islands while simultaneously penalizing island …

Morphology And Habitat Use Among Insular Populations Of The Lizard Anolis Lemurinus From The Cayos Cochinos Archipelago Of Honduras, Michael Latter Logan

Biology Theses - Archive

In Honduras, Anolis lemurinus is distributed along the Atlantic versant of the mainland and on the Caribbean island system consisting of the Bay Islands and Cayos Cochinos archipelagos. In the Cayos Cochinos, A. lemurinus occurs on the two largest islands, Cayo Mayor and Cayo Menor, which are separated by less than 1km. A previous study using genetic (amplified fragment-length polymorphisms) and morphometric (mass and SVL) data suggested that populations of A. lemurinus in the Cayos Cochinos did not differ from each other (Klutsch et al. 2007). Despite the extremely close proximity of Cayo Mayor and Cayo Menor populations, I hypothesized …

How Soil Nutrient Availability Affects Plant Sexual Reproduction And Seedling Recruitment In Alaskan Dry Heath Tundra: Implications For Response To Climate Change, Carol Moulton

Biology Theses - Archive

The harsh growing conditions of the arctic biome, in particular low temperatures and soil nutrient availability, restrict the growth and reproduction of arctic plant species. Recent and ongoing changes in climate are increasing air and soil temperatures in these ecosystems, thus affecting decomposition rates of arctic soils. As decomposition increases, nutrients that are currently immobilized in the soil organic layer become available to nutrient-limited tundra plants. Plant species within the tundra communities then compete for the newly available nutrients, potentially causing a shift in community composition. As an important component of plant life cycles, seeds provide new individuals and genetic …

Crystallization And Crystal-Packing Studies Of Chlorella Virus Deoxyuridine Triphosphatase, Kohei Homma, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

The 141-amino-acid deoxyuridine triphosphatase (dUTPase) from the algal Chlorella virus IL-3A and its Glu81Ser/Thr84Arg-mutant derivative Mu-22 were crystallized using the hanging-drop vapor-diffusion method at 298 K with polyethylene glycol as the precipitant. An apo IL-3A dUTPase with an aminoterminal T7 epitope tag and a carboxy-terminal histidine tag yielded cubic P213 crystals with unit-cell parameter a = 106.65 A . In the presence of dUDP, the enzyme produced thin stacked orthorhombic P222 crystals with unit-cell parameters a = 81.0, b = 96.2, c = 132.8 A. T7-histidine-tagged Mu-22 dUTPase formed thin stacked rectangular crystals. Amino-terminal histidine-tagged dUTPases did not crystallize but …

Role Of Vapbc Toxin–Antitoxin Loci In The Thermal Stress Response Of Sulfolobus Solfataricus, Charlotte R. Cooper, Amanda J. Daugherty, Sabrina Tachdjian, Paul H. Blum, Robert M. Kelly

School of Biological Sciences: Faculty Publications

TA (toxin–antitoxin) loci are ubiquitous in prokaryotic microorganisms, including archaea, yet

their physiological function is largely unknown. For example, preliminary reports have suggested

that TA loci are microbial stress-response elements, although it was recently shown that knocking

out all known chromosomally located TA loci in Escherichia coli did not have an impact on

survival under certain types of stress. The hyperthermophilic crenarchaeon Sulfolobus solfataricus

encodes at least 26 vapBC (where vap is virulence-associated protein) family TA loci in its

genome. VapCs are PIN (PilT N-terminus) domain proteins with putative ribonuclease activity,

while VapBs are proteolytically labile proteins, which purportedly function …

Evolution And Pleiotropy Of Trithorax Function In Arabidopsis, Zoya Avramova

School of Biological Sciences: Faculty Publications

The SET domain-containing genes of the TRITHORAX family encode epigenetic factors that maintain the expression of targeted genes. Trithorax homologs have been found in both animals and plants. Since these are thought to have evolved multicellularity independently, common mechanisms of epigenetic regulation must be evolutionarily ancient and derived from a common ancestor. In addition, each lineage has evolved unique mechanisms to expand the original repertoire of epigenetic functions. Phylogenetic analysis of SET domain proteins has outlined some intriguing evolutionary trends. In plants, epigenetic gene silencing mechanisms have been aggressively pursued. In contrast, studies of epigenetic mechanisms maintaining active gene expression …

Interactive Effects Of Time, Co2, N, And Diversity On Total Belowground Carbon Allocation And Ecosystem Carbon Storage In A Grassland Community, E. Carol Adair, Peter B. Reich, Sarah E. Hobbie, Johannes M.H. Knops

School of Biological Sciences: Faculty Publications

Predicting if ecosystems will mitigate or exacerbate rising CO2 requires understanding how elevated CO₂ will interact with coincident changes in diversity and nitrogen (N) availability to affect ecosystem carbon (C) storage. Yet achieving such understanding has been hampered by the difficulty of quantifying belowground C pools and fluxes. Thus, we used mass balance calculations to quantify the effects of diversity, CO₂, and N on both the total amount of C allocated belowground by plants (total belowground C allocation, TBCA) and ecosystem C storage in a periodically burned, 8-year Minnesota grassland biodiversity, CO₂, and N experiment …

Caenorhabditis Elegans Genomic Response To Soil Bacteria Predicts Environment-Specific Genetic Effects On Life History Traits, Joseph D. Coolon, Kenneth L. Jones, Timothy C. Todd, Bryanua C. Carr, Michael A. Herman

School of Biological Sciences: Faculty Publications

With the post-genomic era came a dramatic increase in high-throughput technologies, of which transcriptional profiling by microarrays was one of the most popular. One application of this technology is to identify genes that are differentially expressed in response to different environmental conditions. These experiments are constructed under the assumption that the differentially expressed genes are functionally important in the environment where they are induced. However, whether differential expression is predictive of functional importance has yet to be tested. Here we have addressed this expectation by employing Caenorhabditis elegans as a model for the interaction of native soil nematode taxa and …

The Nuclear Receptor Nhr-25 Cooperates With The Wnt/Β-Catenin Asymmetry Pathway To Control Differentiation Of The T Seam Cell In C. Elegans, Martina Hajduskova, Marek Jindra, Michael A. Herman, Masako Asahina

School of Biological Sciences: Faculty Publications

Asymmetric cell divisions produce new cell types during animal development. Studies in Caenorhabditis elegans have identified major signal-transduction pathways that determine the polarity of cell divisions. How these relatively few conserved pathways interact and what modulates them to ensure the diversity of multiple tissue types is an open question. The Wnt/β-catenin asymmetry pathway governs polarity of the epidermal T seam cell in the C. elegans tail. Here, we show that the asymmetry of T-seam-cell division and morphogenesis of the male sensory rays require NHR-25, an evolutionarily conserved nuclear receptor. NHR-25 ensures the neural fate of the T-seam-cell descendants in cooperation …

Ecological Genomics Of Nematode Community Interactions: Model And Non-Model Approaches, Michael A. Herman, Joseph D. Coolon, Kenneth L. Jones, Timothy Todd

School of Biological Sciences: Faculty Publications

The effects of human-induced environmental change are evident at multiple levels of biological organization. To date, most environmental change studies have focused on effects at the ecosystem, community, and organismal levels. However, the ultimate controls of biological responses are located in the genome. Thus, genetic and genomic studies of organismal responses to environmental changes are necessary. Recent advances in genome analysis now make such analyses possible. In this chapter we describe a research approach and program that can begin to span this gap by using genome-enabled approaches to characterize organismal changes and then employing a genetically tractable model organism to …

Something Old, Something New, Something Borrowed; How The Thermoacidophilic Archaeon Sulfolobus Solfataricus Responds To Oxidative Stress, Walid S. Maaty, Blake Wiedenheft, Pavel Tarlykov, Nathan Schaff, Joshua Heinemann, Jim Robinson-Cox, Jacob Valenzuela, Amanda Dougherty, Paul H. Blum, C. Martin Lawrence, Trevor Douglas, Mark J. Young, Brian Bothner

School of Biological Sciences: Faculty Publications

To avoid molecular damage of biomolecules due to oxidation, all cells have evolved constitutive and responsive systems to mitigate and repair chemical modifications. Archaea have adapted to some of the most extreme environments known to support life, including highly oxidizing conditions. However, in comparison to bacteria and eukaryotes, relatively little is known about the biology and biochemistry of archaea in response to changing conditions and repair of oxidative damage. In this study transcriptome, proteome, and chemical reactivity analyses of hydrogen peroxide (H2O2) induced oxidative stress in Sulfolobus solfataricus (P2) were conducted. Microarray analysis of mRNA expression showed that 102 transcripts …