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Exploring The Role Of The Id2 And Rab11fip3 Pathway In X-Linked Chronic Granulomatous Disease (Xcgd) Through Comparative Gene Expression Analysis, Kaili Coenen

Honors Theses

X-linked chronic granulomatous disease (XCGD) is a debilitating immunodeficiency disorder primarily affecting men. The disease results from mutations in the CYBB gene on the X chromosome, which encodes the GP91^PHOX protein responsible for electron transfer from NADPH to molecular oxygen. Despite the identification of the CYBB mutation, the pathological mechanism underlying the reduction in reactive oxygen species (ROS) generation in XCGD remains unclear. We hypothesized that comparative gene expression analysis, combined with pathway analysis, could identify genes and signaling pathways contributing to reduced reactive oxygen species (ROS) levels in XCGD. In this study, we analyzed gene expression data from four …

Arthroscopy And The Future Of Surgery: Personal And Public Health Benefits, Preston T. Koski

Honors Thesis

Arthroscopy focuses on operating on the body using probes and working through keyhole incisions on the skin. This rapidly evolving field has seen many innovations in its technology, aided by advances in optical and surgical capabilities. Moreover, it presents many advantages over traditional surgery that directly impact the patient. Shorter recovery time and lessened scarring, reduced postoperative pain and opioid dependence, and less risk of infection are key benefits over open surgery. These benefits can also be applied to areas of public health. Minimizing the burden on hospital resources, reducing the environmental impact of surgery, and improving cost-effectiveness in healthcare …

The Effects Of Prenatal Cigarette Smoke Exposure On Developmental Milestones, Megan Jacobs

College of Arts & Sciences Senior Theses

In Kentucky, a reported 12.7% of mothers smoke cigarettes during pregnancy. Nicotine readily crosses the placental and blood-brain barriers, with fetal blood concentrations reaching 15% higher than maternal levels. By stimulating and desensitizing nicotinic acetylcholine receptors in the autonomic nervous system, nicotine disrupts uteroplacental blood flow, potentially altering fetal growth and development.

To investigate the effects of prenatal cigarette smoke exposure (CSE) on developmental milestones, C57Bl6 mice were exposed to either cigarette smoke (CSE, n = 30) or filtered room air (Sham, n = 20) for 3 hours daily from two days before mating until the day before birth. Offspring …

Firefighters' Perceived Effectiveness Of Peer Support For Job-Related Stressors, Camryn M. Travis

Senior Honors Projects

Firefighters experience immense job-related stress. Firefighters can respond to hundreds of traumatic calls or critical incidents throughout their careers. Witnessing a critical incident can be highly stressful and mentally taxing. Therefore, some fire departments offer mental health resources such as Critical Incident Stress Management/Debriefing (CISM/D) and Peer-group support to help firefighters cope with stressful incidents. Additionally, firefighters may seek professional mental health treatment. To investigate firefighters’ perceptions regarding the effectiveness of these resources, an anonymous survey of Rhode Island firefighters was conducted. The sample included 51 firefighters (n = 51), was mostly male (90.2%), and self-identified as predominantly white (94.1%) …

Pathways Of Nitrogen Loss After Fertilization In Dryland Ecosystems, Fidel Manuel Renteria

Open Access Theses & Dissertations

Dryland ecosystems cover approximately 41% of the Earth’s terrestrial surface, and are characterized by limited moisture, nutrient-poor soils, and pulsed precipitation regimes that collectively constrain nutrient cycling. Although nitrogen (N) is often assumed to be a primary limiting nutrient in these environments, fertilization studies frequently reveal minimal biotic response to added N—a pattern known as the "dryland nitrogen paradox." This paradox has been attributed to rapid nitrogen loss via gaseous emissions and leaching, exacerbated by interactions with water and phosphorus (P) availability. To investigate these loss pathways, we conducted a field experiment at the Jornada Experimental Range in the northern …

Effects Of Cadmium And Diet On Metal Accumulation And Kidney Cell Death Pathways., Justin Xu

College of Arts & Sciences Senior Theses

Cadmium (Cd) is a non-biodegradable environmental toxin and accumulates in the body with an estimated half-life of 30-45 years. Humans can be exposed to Cd through diet, drinking water, tobacco smoking, and industrial pollution. Cd can especially accumulate in the kidney due to the release of Cd from protein-metal complexes when filtering blood in the tubules causing oxidative stress and inducing kidney injury. Obesity is another risk factor for kidney disease therefore we examined the dual effects of Cd and obesity on the accumulation of essential metals in mouse kidneys. Starting in utero and continuing until 24 weeks postweaning, male/female …

Understanding The Properties Of Repair Protein P26 For Dry-State Preservation Of Biological Materials., Hannah Skaggs

College of Arts & Sciences Senior Theses

The preservation of complex biological materials in the frozen state has been well established and plays a critical role in the advancement of clinical applications and the pharmaceutical industry. Cryopreservation of complex biological materials has several clinical applications, including vaccine preservation, bone marrow transplants, cell therapeutics, and increasing the shelf life of red blood cells (RBCs) for transfusion. However, many challenges are present, including the use of toxic protective agents and the financial burden of maintaining extremely low temperatures between 193.15 and – 77.6 K (Parihar, Kumar et al. 2023). Lyophilization, or freeze-drying, presents an opportunity to mitigate such challenges. …

Recombinant Expression And Purification Of Porphyromonas Gingivalis Tyrosine Kinase 1 In Escherichia Coli., Laura K. Hanna

College of Arts & Sciences Senior Theses

The goal of this study was to optimize the expression and purification of Porphyromonas gingivalis Tyrosine Kinase 1 (Ptk1) in recombinant Escherichia coli containing the plasmids Gro_ESL and Ptk1, with a His₆ tag_, to obtain a highly pure protein sample. Various experimental conditions were tested to determine the most effective parameters for protein expression and purification, including adjustments in induction timing, buffer composition, and purification techniques.

Optimal protein expression was achieved when cells were induced at an optical density (OD) of 0.3 to 0.4. β-D-1-thiogalactopyranoside (IPTG), which activates the lac operon, was used to induce Ptk1 expression. A …

Growth Of Methanogens Using Pyrite As An Energy Source: Implications For Life On Mars, Mckenzie K. Ragan

Biological Sciences Undergraduate Honors Theses

The search for life beyond Earth has been the motivating factor for many scientist’s research in the past and currently. Specifically, the efforts have largely been focused on Mars. Mars exhibits qualities that are similar to the life-harboring characteristics of Earth, creating the possibility for life to exist on Mars. Mars’ subsurface conditions may be ideal for specific microorganisms to thrive, providing a haven away from the harsh surface conditions (Mancinelli, 2000). It is known that one of the most important elements to support life, H₂O is found in the subsurface of Mars, along with an atmosphere lacking …

Metabolic Responses Of Adipose-Derived Stem Cells In Collagen I Hydrogels, Caroline R. King

Biological Sciences Undergraduate Honors Theses

Peripheral nerve injury affects approximately 44 in 1 million people in the United States every year and can lead to incomplete nerve recovery and loss of motor and sensory function. A significant amount of energy is required for nerve injury repair. A lack of energy allocation to the injury site hinders repair. Current treatments include hollow nerve guidance conduits, decellularized nerve grafts, and autografts. However, they are limited by factors such as donor site morbidity and sustained immunosuppressive treatments. Using three-dimensional collagen I hydrogels laden with adipose-derived stem cells (ASCs) can assist in neural regeneration. Three-dimensional collagen scaffolds aim to …

The Expression Of Intestinal Tight Junction Proteins Induced By 1,25-Dihydroxyvitamin D3-Glycosides Supplementation To Reduce Bacterial Chondronecrosis With Osteomyelitis Lameness In Broilers, Kennedy M. Hicks

Biological Sciences Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a leading cause of lameness in broiler chickens, impacting animal welfare and commercial poultry production. Supplementation using organic trace minerals, vitamin D, probiotics, and prebiotics has been studied to mitigate BCO lameness incidences in broiler chickens. Our previous study demonstrated that feeding Panbonis®-G-1,25(OH)₂D₃ from Solanum glaucophyllum, containing 1 µg/kg G-1,25(OH)₂D₃, to broilers for the first 28 days of their production cycle reduced BCO lameness by approximately 50%. In this study, we investigated the impact of 1,25-dihydroxy vitamin D₃ supplementation on the expression of tight junction …

Immune Function Modulation By Temperature On Isolated Rainbow Trout Macrophages, Abby Hunter

Biological Sciences Undergraduate Honors Theses

Fever is an evolutionary mechanism that enhances immune function, yet the molecular and metabolic responses to fever temperatures in ectothermic species remain poorly understood. In this study, we investigated how fever temperatures (19° C) influence macrophage function and metabolic activity in rainbow trout (Oncorhynchus mykiss), rather than normal temperatures (16° C). Using a rainbow trout monocytic cell line, RTS11, and primary head kidney-derived macrophages (HKCs), we examined the effects of fever temperatures on nitric oxide (NO) production, gene expression, protein synthesis, and energy metabolism. We found that fever temperatures increased NO production in both cell types, with further …

The Impact Of Dimerization And Condensate Formation On Notch Signaling Outcomes, Tyler Michael William Lawton

Boise State University Theses and Dissertations

Communication between cells is the cornerstone of multicellular life. This communication is facilitated through signal transduction, wherein an environmental que activates a transducer on the surface of the cell or within it. This signal is recognized and transmitted in a way that integrates every other signal that the cell is receiving at the time, culminating in a dynamic dance of molecular components producing finely tuned responses to the cellular environment. Notch signaling facilitates direct communication between neighboring cells, as well as response to various microenvironmental stimuli, to influence cell differentiation and fate. As insinuated above, these communication pathways do not …

Antimicrobial Peptides Improve Antibiotic-Mediating Killing Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Biofilms, Alya R. Theriault

All Theses And Dissertations

Staphylococcus aureus can cause drug-resistant, biofilm-associated infections. These infections place methicillin-resistant S. aureus (MRSA) as one of the sixth leading bacterial pathogens associated with deaths due to antimicrobial resistance. Traditional antibiotics are no longer enough to treat these resistant infections and novel therapeutics are needed. Antimicrobial adjuvants are a potential strategy for improving efficacy of existing antibiotics, and antimicrobial peptides (AMPs) may have adjuvant activity. Recent reports showed that AMPs improve antibiotic activity against planktonic staphylococci, but whether AMPs might alter antibiotic efficacy against biofilm-resident staphylococcus is less clear. We tested the effect of nine AMPs on staphylococcal biofilm susceptibility …

A Genetic And Spatial Analysis Of The Last Megafaunal Species (Tapirus Terrestris) In The Paraguayan Dry Chaco Forest, Emily Bowers

LSU New Orleans Theses and Dissertations

No abstract provided.

The Effects Of Pfos Exposure On Zebrafish Dopaminergic Neurodevelopment, Allison G. Fletcher

Departmental Honors & Graduate Capstone Projects

Per- and polyfluoroalkyl substances (PFAS) are a group of environmental contaminants that cause disruptions to the nervous and endocrine systems. Perfluorooctanesulfonate (PFOS) is considered one of the most disruptive PFAS to the nervous system, and may disrupt neurodevelopment of the dopaminergic system, which has been linked to neurodevelopmental disorders like Attention Deficit/Hyperactivity Disorder (ADHD) in humans. I hypothesized that early life PFOS exposure disrupts the development of the dopaminergic system. To test this hypothesis, I exposed zebrafish to 0.05, 0.5, and 5.0 mg/L PFOS through 5 days of embryonic and larval development. To assess acute effects of PFOS on the …

Investigating The Effects Of Sod1 Knockout On Oxidative Stress And Stress Coping Styles In Zebrafish Using Crispr Technology, Tai Prauner, Ryan Wong

Theses/Capstones/Creative Projects

Reactive oxygen species are byproducts of cellular metabolism that, when not efficiently neutralized, can damage DNA, proteins, and lipids. Superoxide dismutase 1 (SOD1) is an antioxidant enzyme that converts superoxide radicals into less harmful molecules. While its molecular function is well documented, the role of SOD1 in shaping stress-related behavior remains underexplored. This project aimed to establish foundational tools for studying the behavioral impact of SOD1 knockout across proactive and reactive stress-coping styles in zebrafish.

To support future gene-editing experiments, microinjection and breeding protocols were refined across wildtype AB, proactive, and reactive zebrafish lines. No embryos were injected with CRISPR-Cas9 …

Ghosts In Glass: Ghost Crabs As Judges Of Glass Sand For Coastal Restoration, Emily Parrish

Honors Theses

Over 60% of the Gulf of Mexico coastline is actively eroding, which has resulted in thousands of miles of lost coastline over the last 100 years. This drastic loss of coastal land highlights the dire need for coastal restoration across the entirety of the Gulf Coast. A vital aspect of restoring these eroded areas is replenishing eroded substrate, traditionally conducted through offshore dredging of substrates which comes with high economic and ecological costs. Recycled glass sand may be able to fill this role and reduce our reliance on dredging while lowering the amount of glass waste entering landfills each year. …

Impact Of Temperature And Microbial Infection On The Honey Bee Hemocytes, Michael E. Oeth

Master's Theses

Introduction

Honey bees (Apis mellifera) rely on hemocytes for immunity, wound healing, and hemolymph homeostasis. While 32°C reflects optimal hive conditions, suboptimal temperatures (22°C) and stressors like pathogens (Varroa destructor, bacteria) or pesticides impair hemocyte function. Using clodronate liposomes (CLD) to deplete hemocytes, we assessed how immune suppression interacts with these stressors.

Methods

Newly emerged (5-day-old) and older (15-day-old) bees were assigned to: CLD, control liposomes (LP), PBS, injection wound, or no treatment. Cohorts were exposed to 32°C (optimal) or 22°C (stressful for non-immunosuppressed bees). Fifteen-day-old bees were challenged with Escherichia coli or Staphylococcus aureus. Survival, hemolymph volume, and hemocyte …

Synthetic Modification Of Agrobacterium To Inhibit Plant Pathogenesis, Rosemala Agarwala

Honors Theses

Crown Gall disease caused by Agrobacterium is a leading cause of plant damage and current solutions used to prevent the disease are not as effective. This research aims to use a synthetic biology approach to design a plasmid that upregulates Curdlan, a molecule that activates plant defenses, when the bacteria senses a wounded plant. Thus this research uses a synthetic biology approach to develop a biocontrol agent for Crown Gall disease. This research also looks at the transferability of the sugar phenol sensing mechanism in Agrobacterium, which is needed for plant pathogenesis.

Substrate Complexity Is Not Correlated With Skin Pattern Complexity In Callistoctopus Furvus, Emily Lekas

Honors Theses

Callistoctopus furvus is an understudied, nocturnal octopus that inhabits shallow, subtropical waters in the western Atlantic. This species relies on camouflage to blend in to diverse environments to escape predators. To understand the relationship between substrate and skin patterning in this species, we used second-by-second analysis of video data recorded in the field. We compared frequencies of four substrate types (sand, rock, algae, and seagrass) ranked by contrast across ten skin patterns displayed by C. furvus. Chi-squared analyses showed significant results that the four substrates were unevenly represented in every skin pattern. However, the data did not support our hypothesis …

Ghrelin And Decision-Making: Exploring Its Role In Food Reward, Novelty-Seeking, And Cost Valuation, Serina A. Batson

Open Access Theses & Dissertations

When low energy levels are detected, Ghrelin, a peptide hormone, is produced in the fundus of the stomach. Referred to as the "hunger hormone", Ghrelin is traditionally associated with increased food intake and blood sugar levels. A key modulator in the gut-brain interaction, Ghrelin's growth hormone secretagogue receptor can be found widely across the cortico-striosomal circuit. Critical for decision-making, Ghrelin-based manipulation of this circuit can directly affect reward and cost valuation. While Ghrelin is uniformly considered to increase the valuation of food-based rewards, little research has explored its impact on non-food-based rewards or its effects on cost valuation. Therefore, to …

A Cross-Taxa Comparison Of Avian Telomere Dynamics, Age, And Lifespan, Benjamin Konigsberg

Honors Theses

When eukaryotes replicate cells throughout their life, the end regions of chromosomes naturally degrade as a result of the 5' to 3' directionality of DNA polymerase. There is a point when the chromosome is too damaged to provide normal cellular functions, so the cell will die. Telomeres act as a buffer by providing repetitive sequences of non-coding nucleotides. While telomeres do shorten during replication events, the enzyme telomerase can extend telomeres to prevent senescence. Telomere lengths vary between individuals based on different stressor and stress mitigation. Telomeric rate of change is a metric that can capture telomerase activity by measuring …

Quantification Of Blue Carbon Within The Marine Animal Forest On The Rio Grande Valley Artificial Reef, Michelle Kathryn King

Theses and Dissertations

The protection and enhancement of coastal ecosystems provides a number of benefits such as heightened biodiversity, increased fisheries, and enhanced carbon capture (Yeemin et al., 2024). The study of blue carbon ecosystems has expanded rapidly in the last two decades since the term was coined in 2009 (Lovelock & Duarte, 2019). The most well-known blue carbon ecosystems include vegetation-dominated ecosystems such as seagrass meadows, salt marshes, and mangrove forests (Howard et. al., 2014). Prior studies have overlooked Marine Animal Forests (MAF) ability to capture carbon, until recent studies on soft coral (Coppari et al., 2019) and sponge (Rix et al., …

Pastoral Land Condition: Kimberley Land Condition Standards, Robert Sudmeyer, Rick Fletcher, Kathryn Ryan, Matthew Fletcher, Charles Martin, Philip Thomas, Karen Holmes, Karyn Reeves, Wayne Fletcher

Natural resources published reports

This report outlines the conceptual basis and quantitative land condition standards that define acceptable pastoral land condition for the Kimberley, Western Australia (WA), to meet the regulatory requirements of the Land Administration Act 1997 and the Soil and Land Conservation Act 1945.

The Department of Primary Industries and Regional Development (DPIRD) develops regional-level pastoral land condition standards for WA to implement the Framework for sustainable pastoral management – Revised edition, which is designed to address the Office of the Auditor General’s recommendations to improve the management of pastoral lands in WA. The application of these standards is expected to …

The Effect Of Calorie Restriction On Humoral Responses Towards The Influenza Vaccine, Grayson Kobluk

Celebrating Scholarship and Creativity Day (2018-)

Calorie restriction (CR) - a reduction in caloric intake without malnutrition - has been widely studied for its impact on lifespan and the onset of age-related diseases. Emerging research indicates that CR also influences immune function, though its effects can vary depending on the degree of restriction, duration, and individual characteristics. This study examined how CR effects vaccine-specific antibody responses following influenza vaccination. Male and female mice were maintained on either a control diet or a calorie-restricted diet and vaccinated with an inactivated influenza virus vaccine. Antibody titers were measured 28 days post-vaccination to evaluate the magnitude of the humoral …

Life In Plastic? Not So Fantastic: Marine Plastic Pollution On San Salvador Island, Dayna Wiley

Celebrating Scholarship and Creativity Day (2018-)

Marine plastic pollution, also known as marine macroplastic debris (MMD), impacts many aspects of marine ecosystems. MMD is defined as macroplastic (> 5mm) objects that are deposited in the ocean and are found floating in the water column or washed up on beaches. MMD washup is significantly affected by ocean and wind currents. The Antilles Oceanic Current and the Trade Winds move west across the Atlantic Ocean, and thus were predicted to cause more MMD deposition on the eastern side of San Salvador Island, Bahamas. Beaches on the North (Graham’s Harbor), South (French Bay), East (East Beach), and West (Columbus …

Exercise Physiological Insights Into Exercise-Induced Muscle Cramping: Blood Lactate And Creatine Kinase As Biomarkers In College Students, William Yu

Celebrating Scholarship and Creativity Day (2018-)

Creatine kinase (CK) is an enzyme that's found in the skeletal muscle, heart muscle and brain. When any of these tissues are damaged, they leak creatine kinase into your bloodstream. Elevated CK levels may indicate muscle injuries, cramping, or disease1. There are different types of CK, which are also known as isozymes for the cytoplasm, CK-MM, CK-MB, and CK-BB. CK-MM focuses on musculoskeletal, CK-MB focuses on heart muscles, and CK-BB focuses on the brain tissue. There are also two isozymes in the mitochondria15. Cramping is common problem amongst runners and can be described as “painful, spasmodic contraction of the skeletal …

The Effects Of Climate Change On The Spread Of Mosquito-Borne Diseases, Lauren Provoncha, Sarah Franta, Portia Lawrence

Celebrating Scholarship and Creativity Day (2018-)

This paper examines the impact of climate change on the spread of mosquito-borne diseases, focusing on how rising temperatures, altered precipitation patterns, and extreme weather events influence mosquito habitats and disease transmission. It explores the ecological and public health implications of these shifts and highlights the importance of understanding climate-disease dynamics to inform effective prevention and intervention strategies.

Ecological Consequences Of Variable Glacial Melting On Arctic Biodiversity: Trophic Shifts And Community Dynamics, Aislyn Malone, Audrey Swart

Celebrating Scholarship and Creativity Day (2018-)

Our project investigated the profound impacts of increasing glacial melting on various trophic levels, habitats, and other ecological processes.