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Polymorphism In The Prpc Prion Protein Gene In Pigs, Anuja Paudyal

Master's Theses or Doctor of Nursing Practice

Protein coding regions of the PrPC gene in 51 pigs belonging to 3 breeds namely Hampshire, Yorkshire, Blue Butt Cross (blue spotted cross from a Hampshire X Yorkshire), and a wild pig were studied. These breeds are used nationwide for commercial purposes. The PrPC gene of the pigs that code for prion proteins were sequenced and studied. Deletions of the octapeptide (WGQPHGGG) were observed in 13 samples that belongs to the crossbreed Dark Cross sow 71 by Blue Butt Boar (Gummy bear). This hydrophobic repeat has been used in experiments as a model to investigate neurodegeneration in prion diseases. Also, …

Role Of Ime4 And Pho92 Proteins In Pho5 Regulation, Krishna Shah

Senior Honors Theses

In the yeast Saccharomyces cerevisiae, m6A methylation regulates the stability and translation of mRNA. Ime4, which is highly active in meiotic cells, recognizes consensus methyl sites in pre-mRNA and adds methyl groups to a small fraction of adenosine residues, whereas Pho92 binds to such methylated sites and promotes subsequent degradation. PHO5 transcripts expression is upregulated when IME4 and PHO92 are deleted. In the PHO5 transcript, near the stop codon of the open reading frame (ORF), there is a potential consensus methyl site that is predicted to be methylated, based on its location and excellent match to the consensus methylation sequence …

Multifunctionality Of Cover Crops On Organic Vegetable Farms In South Texas, Savannah M. Rugg

Theses and Dissertations

Situated in deep South Texas, the Lower Rio Grande Valley (LRGV) is considered one the most productive agricultural regions in the southern US. With the highest concentration of organic farms in the state (Hidalgo county), finding management practices that comply with organic certification is increasingly pertinent. Cover cropping can serve multiple functions in an agroecosystem such as: suppressing weeds, increasing soil organic matter (SOM), increasing soil nitrates, and enhancing soil biodiversity. The four cover crops (lablab, sunn hemp, sudangrass, and pearl millet) were assessed to see their potential to increase soil nitrogen, to increase soil organic matter, and to suppress …

Transcriptional Regulation Of Dksa P3 Promoter In Escherichia Coli, Chibuokem Ikwuazom

Biological Sciences

DksA, a global gene regulator, binds to RNA po lymerase to regulate transcription initiation in Escherichia coli . It negatively regulates transcription of some genes, such as those for ribosomal RNAs and fis , and positively regulates transcription of other genes, including genes involved in amino acid biosynthesis. Little is known about how DksA itself is regulated. Current work in our lab has shown three major promoters, a σ 70 dependent P 1 promoter and two σ 38 (RpoS) - dependent promoters (P 2 and P 3 ), regulate the transcription of dksA . More is current ly known about …

Range-Wide Prevalence And Impacts Of Pseudocercosporella Inconspicua On Lilium Grayi And An Assessment Of L. Superbum And L. Michauxii As Reservoirs, Cindy L. Barrett

Electronic Theses and Dissertations

Lilium grayi (Gray’s Lily), a southern Appalachian endemic species, is threatened by a Lilium-specific fungal pathogen, Pseudocercosporella inconspicua. The disease is characterized by tan lesions that can cause early senescence, while also lowering seed production and viability. This project tested for P. inconspicua conidia and accessed health at nine locations. The disease was present and ubiquitous across the range of L. grayi. Through identification of P. inconspicua conidia in the field, L. superbum (Turk’s Cap Lily) was identified as an additional host, while L. michauxii (Michaux’s Lily) was disease-free. However, infection was inducible in both species. With …

The Bovine Rumen Microbiome Revealed By Different Fractions Of Rumen Contents, Ashlee L. Breakstone

Animal Science Undergraduate Honors Theses

The bovine rumen microbiota is very important in terms of animal functionality and digestion. The fermentative capability of the rumen provides means for the digestion of complex plant material that is indigestible by humans. The rumen is the working ground for millions of microorganisms, primarily bacteria, to digest and ferment feed into volatile fatty acids and bacterial protein for the animal’s energy and protein needs, respectively. Of significant importance is the rumen microbiomes ability to conform to certain factors such as genetics, feed, or geographic location. These changes can have a direct measure on animal growth, health, and performance. The …

Dietary Supplementation With Tart Cherries For Prevention Of Inflammation-Associated Colorectal Cancer In Mice, Ashlie Hunter

Undergraduate Honors Capstone Projects

The cherry fruit is a nutrient-dense food with comparatively low caloric content and significant amounts of key nutrients and bioactive food chemicals. Much of the health benefit of cherries is attributed to their high amounts of anthocyanins, which have anti-oxidant and anticancer properties that contribute to changes in cell signaling pathways involved in inflammation, carcinogenesis and angiogenesis. In this project, we aimed to determine whether dietary supplementation with tart cherries prevents colon tumor development in mice consuming a Western diet compared to a prudent diet. Previously, our research team developed the Total Western Diet (TWD) for mice to emulate typical …

Interactions Between Plant Water-Stress And Neonicotinoid Insecticides On Spider Mite Infestations In Corn, Alice Ruckert

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spider mites (Acari: Tetranychidae) are an important agricultural pest of many crops and landscape plants. They can reproduce rapidly and quickly develop resistance to many pesticides, making them difficult to manage. Plant water-stress and high temperatures promote spider mite infestations, while spider mite outbreaks can also result from neonicotinoid insecticide applications. Drought is predicted to increase in the Intermountain West due to increases in temperature and reduced frequency of precipitation events in the region, and neonicotinoids are currently one of the most widely used classes of insecticides in field crops. I studied the interactive effect of these two simultaneously occurring …

The Role Of The Fatty Acid Signaling Pathway In Dietary-Induced Obesity, Melissa N. Nelson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In recent years, dietary fat has been shown to be capable of activing taste receptor cells in the tongue. Fatty acids (FAs), which act as the chemical cue and are found in dietary fat, activate a cellular signaling pathway that results in a unique signal being sent to the brain that is then interpreted as the taste of fat. One important element in this pathway is the ion channel TrpM5. It is responsible for depolarizing the taste cells that are activated by fatty acids; depolarization is an essential step in cellular response, making TrpM5 essential in the functioning of the …

Spatial Ecology Of The Adult And Neonate Eastern Box Turtle In A Forested Landscape, Patrick B. Laarman

Masters Theses

A thorough understanding of spatial ecology is fundamental when developing and implementing conservation strategies for imperiled turtle species. I investigated spatial ecology of adult and neonate eastern box turtles in the Manistee National Forest (MNF), Michigan. My primary objectives were to evaluate relative habitat preferences of adults and document residency time of neonates in natal openings. I radio-fitted 25 adults, protected 64 nests, and radio-fitted 66 neonates. Mean home range size for adults (n = 25 turtles) was 16.4 ha ± 2.4 SE (100% Minimum Convex Polygon). I detected non-random habitat use by adults (Wilks Ʌ = 0.202, df = …

Student Misconceptions About Plants – A First Step In Building A Teaching Resource, April N. Wynn, Irvin L. Pan, Elizabeth E. Rueschhoff, Maryann Herman, Kathleen Archer

Biology Faculty/Staff Publications

Plants are ubiquitous and found in virtually every ecosystem on Earth, but their biology is often poorly understood, and inaccurate ideas about how plants grow and function abound. Many articles have been published documenting student misconceptions about photosynthesis and respiration, but there are substantially fewer on such topics as plant cell structure and growth; plant genetics, evolution, and classification; plant physiology (beyond energy relations); and plant ecology. The available studies of misconceptions held on those topics show that many are formed at a very young age and persist throughout all educational levels. Our goal is to begin building a central …

Genome Sequence Of Staphylococcus Saprophyticus Dpc5671, A Strain Isolated From Cheddar Cheese, Caitríona M. Guinane, Et. Al.

Department of Biological Sciences Publications

The draft genome sequence of Staphylococcus saprophyticus DPC5671, isolated from cheddar cheese, was determined. S. saprophyticus is a common Gram-positive bacterium detected on the surface of smear-ripened cheese and other fermented foods.

GENOME ANNOUNCEMENT

Nutritional Effects On Age-Related Changes In T-Cell Differentiation And Function, Michelle Chen

Whittier Scholars Program

T-cells are a type of white blood cell that in our bodies, scanning for cellular abnormalities and infections, thus making them essential for human immunity. However, due to the effects of aging, our bodies diminish the production of T cells and as a result, elderly individuals do not respond to immune disturbance as robustly as young individuals. In this experiment, I am examining the Jurkat T-cell, a human T lymphocyte cell line, viability at different treatment concentrations of the natural compounds, resveratrol and curcumin, and two cancer treatment drugs, cisplatin and bleomycin. These natural compounds, resveratrol and curcumin, have been …

Innate Food Preference In The Larval Tobacco Hornworm, Manduca Sexta, Michael Morrison

Thinking Matters Symposium Archive

Food preference can drive an organism to seek a specific food source even if another food, which can provide needed nutrition, is easier to obtain. Food preference can develop in different ways, including innate preference, i.e., organisms display food preference at birth, or learned preference, i.e., organisms develop food preference after previous experience with that food. The tobacco hornworm, Manduca sexta, shows learned preference; however, we lack information about innate food preference in Manduca. Manduca sexta larvae eat many different foods until they feed on a solanaceous plant, when they become more specific in their diet. This study focused on …

It’S All About Relationships: Amf-Plant Associations, The Rhizosphere, And Our Changing World, Joel Velasco

McNair Scholars Research Journal

Arbuscular mycorrhizal fungi are obligate biotrophic organisms that facilitate nutrient uptake in exchange for organic carbon as part of a symbiotic association with their host plants. This symbiotic relationship is among the oldest known and has been important for the coevolution of both symbionts. This review explores the relationship between Arbuscular mycorrhizal fungi and their hosts, the role AMF play in the invasive strategies of certain plants, and how severe pressure affects the reciprocal relationship. This review hopes to add to the body of knowledge and to the conversation about these dynamic relationships and the timescale on which they operate, …

Student Misconceptions About Plants – A First Step In Building A Teaching Resource, April N. Wynn, Irvin L. Pan, Elizabeth E. Rueschhoff, Maryann A B Herman, E Kathleen Archer

Biological Sciences Research

Plants are ubiquitous and found in virtually every ecosystem on Earth, but their biology is often poorly understood, and inaccurate ideas about how plants grow and function abound. Many articles have been published documenting student misconceptions about photosynthesis and respiration, but there are substantially fewer on such topics as plant cell structure and growth; plant genetics, evolution, and classification; plant physiology (beyond energy relations); and plant ecology. The available studies of misconceptions held on those topics show that many are formed at a very young age and persist throughout all educational levels. Our goal is to begin building a central …

Abiotic Conditions Of Rare Pitcher's Thistle Attract Selection By An Invasive Weevil, Monica Paniagua Montoya

Lawrence University Honors Projects

Plant associations occur when host selection of a focal plant is reduced or increased by the presence of another plant species. Larinus planus is an invasive weevil whose larvae feed in the flower heads of native and federally threatened Pitcher’s thistle (Cirsium pitcheri). Studies have linked the presence of neighboring plants to increased host selection of Pitcher’s thistle by the weevil, but the factors contributing to host selection remain unknown. In our study, we determined if there were differences among abiotic factors, host selection and weevil behavior at Pitcher’s thistles surrounded by beach grass, sand and at high …

Differential Toxic Effects Of Ulva Lactuca (Chlorophyta) On The Herbivorous Gastropods, Littorina Littorea And L. Obtusata (Mollusca), Paulette Peckol, Alysha B. Putnam

Biological Sciences: Faculty Publications

Members of the genus Ulva are widespread and abundant in intertidal and shallow subtidal areas but there are conflicting data regarding susceptibility to herbivory. While some studies have documented that Ulva spp. were favored by a diversity of marine herbivores, other work has revealed herbivore deterrence. We investigated grazing and growth rates of the littorinid species, Littorina littorea and L. obtusata, when offered Fucus vesiculosus, Ascophyllum nodosum, Ulva lactuca, and Chondrus crispus, highlighting distinctive vulnerabilities to toxic effects of U. lactuca. Ulva lactuca was the preferred food of L. littorea, while L. obtusata showed no grazing on this ephemeral algal …

Ecological Correlates With Dioecy In The Flora Of A Tropical Premontane Wet Forest In Costa Rica., Breanne Held

Undergraduate Distinction Papers

There is a considerable disagreement about the selective forces that have led to the evolution of dioecy (i.e., the condition of having separate male and female flowers on separate plants). Historically, selection to enforce outcrossing has been the primary explanation despite there being little empirical evidence. Hence, many scientist argue that the selection of dioecy is driven by ecological traits that influence male and female fitness and seed dispersal. The objective of this study was to examine the association between plant sexual system (particularly, dioecy) with various ecological traits for the flora of a tropical premontane wet forest in Costa …

Effects Of Ultrasound On Amyloid Beta 42 (Aβ42) Mediated Neurodegeneration, Sarah Byrne

Honors Theses

Alzheimer’s disease (AD) is an age related progressive neurodegenerative disease. The exact mechanisms that lead to cell death are not entirely understood. It has been shown that accumulation of amyloid-beta-42 (Aβ42) plaques generated by mis-cleavage of amyloid-precursor-protein is the cause of neurodegeneration seen in AD. This is due to the hydrophobic nature of Aβ42 due to extra two amino acids added to the typical and naturally occurring Aβ40 in the body. These Aβ42 plaques trigger neuronal death because of the toxic nature and stress they exert on the neurons. In this study, Drosophila melanogaster transgenic model where human Aβ42 coding …

Insights Into Stat5 Biology, Taylor Millar

Honors Capstones

No abstract provided.

Urban Soils In Response To Flooding, Katherine Henry

Honors Capstones

No abstract provided.

Got Bugs In Your Kefir?, Julienne Henrie

Biology Posters

What is Kefir ? Kefir is a type of fermented milk that is cultured with Kefir grains. Kefir grains make up the probiotic culture used to produce Kefir. These cauliflower-like grains contain mostly lactobacilli bacteria and yeasts held together by Kefiran, an exopolysaccharide structure. (Korsak et al. 2014). Kefir grains are Mesophilic. Mesophilic starters are cultured at room temperature, which allows the probiotics to remain active when consumed. Properties of mesophilic starters also give Kefir the liquid consistency it is known for. (Sarah, 2016). Kefir originated in the Caucasus Mountains thousands of years ago, the name coming from the Turkish …

Social Amoebae Mating Types Do Not Invest Unequally In Sexual Offspring, T E. Douglas, David C. Queller, Joan E. Strassmann

Biology Faculty Research

Unequal investment by different sexes in their progeny is common and includes differential investment in the zygote and differential care of the young. The social amoeba Dictyostelium discoideum has a sexual stage in which isogamous cells of any two of the three mating types fuse to form a zygote which then attracts hundreds of other cells to the macrocyst. The latter cells are cannibalized and so make no genetic contribution to reproduction. Previous literature suggests that this sacrifice may be induced in cells of one mating type by cells of another, resulting in a higher than expected production of macrocysts …

Interview With Eileen Gregory, Eileen Gregory, Wenxian Zhang

Oral Histories

Eileen Gregory received her BS from Michigan State University, an MS & PhD in microbiology from The University of Washington, where her research focused on freshwater bacteria. As a young professor in Biology, Eileen Gregory came to Rollins College in 1979, one of two women in the department’s faculty of five, when the sciences were dominated by men. She never expected to be at Rollins all her professional career: “I came to Rollins with the idea that I would stay here for three years and move on,” Gregory says. “But I love my colleagues; I love my students; and for …

Consistency Of Structural Color Across Molts: The Effects Of Environmental Conditions And Stress On Feather Ultraviolet Reflectance, Rebecca Lynn Windsor

USF Tampa Graduate Theses and Dissertations

Across avian systems, plumage ornamentation is often considered a signal of individual fitness, condition, sex, or status, and varies due to genetics or environmental sources. In species with structural coloration, plumage variation results from differences in the amount of energy allocated to feather growth during molt, presenting a unique opportunity to study the link between individual quality and ornamentation. In cooperative breeding species, such as the Florida Scrub-Jay (Aphelocoma coerulescens), the role of structural color as a signal is particularly important because helpers may delay breeding for one to several years and competition for space is high. Florida Scrub-Jay juveniles …

Collaborative Research: The Interplay Between Fluctuating Selection On Symbionts And Life Cycle Evolution In Sap-Sucking Insects, Carol Von Dohlen

Funded Research Records

No abstract provided.

High-Fat Feeding Does Not Disrupt Daily Rhythms In Female Mice Because Of Protection By Ovarian Hormones, Brian T. Palmisano, John M. Stafford, Julie S. Pendergast

Biology Faculty Publications

Obesity in women is increased by the loss of circulating estrogen after menopause. Shift work, which disrupts circadian rhythms, also increases the risk for obesity. It is not known whether ovarian hormones interact with the circadian system to protect females from obesity. During high-fat feeding, male C57BL/6J mice develop profound obesity and disruption of daily rhythms. Since C57BL/6J female mice did not develop diet-induced obesity (during 8 weeks of high-fat feeding), we first determined if daily rhythms in female mice were resistant to disruption from high-fat diet. We fed female PERIOD2:LUCIFERASE mice 45% high-fat diet for 1 week and measured …

Competition From Bromus Tectorum Removes Differences Between Perennial Grasses In N Capture And Conservation Strategies, Rebecca E. Drenovsky, Jeffrey T. Walker, J. J. James

Biology

Background and aims

Competition from the annual grass Bromus tectorum threatens aridland perennial bunchgrass communities. Unlike annuals, perennials must allocate part of their first year nitrogen (N) budget to storage rather than growth, potentially placing them at a competitive disadvantage.

Methods

We evaluated N acquisition and conservation for two perennial bunchgrasses, Agropyron desertorum and Pseudoroegneria spicata, at the seedling stage to investigate potential trade-offs between storage and growth when grown with and without B. tectorum under two levels of soil N.

Results

Agropyron desertorum had higher growth rates, N uptake, and N productivity than P. spicata when grown without …

Estimating Seasonal Variation In Condition Of Yellow Perch, Emily Anne Powers

Biology Graduate Theses

Research has shown that Yellow Perch Perca flavescens, found throughout lakes and streams of North America, are good indicators of environmental health. Due to the correlation between fish condition and ecosystem health it is essential to reliably estimate condition to better manage our fisheries. Therefore, the study objectives were to measure the seasonal changes in adult Yellow Perch percent dry weight (PDW), and compare the reliability of bioelectrical impedance analysis (BIA) and morphometric-based estimates of condition. The seasonal fluctuations in average monthly PDW were measured over a two- year period and were sinusoidal in shape with peaks in August 2015 …