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Heritable Sperm Chromatin Epigenetics: A Break To Remember, Ralph G. Meyer, Chelsea C. Ketchum, Mirella L. Meyer-Ficca

UAES Publications

Sperm chromatin not only has a unique structure to condense and protect the paternal DNA in transit, but also provides epigenetic information that supports embryonic development. Most of the unique sperm nuclear architecture is formed during the sweeping postmeiotic chromatin remodeling events in spermiogenesis, where the majority of nucleosomes are removed and replaced by protamines. The remaining histones and other chromatin proteins are located in structurally and transcriptionally relevant positions in the genome and carry diverse post-translational modifications relevant to the control of embryonic gene expression. How such postmeiotic chromatin-based programming of sperm epigenetic information proceeds, and how susceptible the …

Morganella Morganii (Enterobacteriales: Enterobacteriaceae) Is A Lethal Pathogen Of Mexican Fruit Fly (Diptera: Tephritidae) Larvae, Bacilio Salas, Hugh E. Conway, Erin Schuenzel, Kristen Hopperstad, Christopher Vitek, Don C. Vacek

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Tephritid pests, such as the Mexican fruit fly, Anastrepha ludens (Loew), represent a major threat to fruit production worldwide. In order to control these pests, sterile insect technique is used to suppress and eradicate wild populations. For this control method to be successful, hundreds of millions of flies must be produced weekly in mass rearing facilities. The large quantity of artificial diet and close proximity of flies at various life stages allows bacteria from family Enterobacteriaceae, Bacillaceae, Pseudomonadaceae, and others to multiply and spread more easily. In this study, bacteria with a possible pathogenic effect were isolated from Mexican fruit …

Small Rna-Dependent Gene Silencing In The Green Alga Chlamydomonas Reinhardtii: Functions And Mechanisms, Eun Jeong Kim

School of Biological Sciences: Dissertations, Theses, and Student Research

Small RNAs (sRNAs), ~20-30 nucleotides in length, are non-coding RNAs that play essential roles in the regulation of gene expression in eukaryotes. They lead to inactivation of cognate sequences at the post-transcriptional level via a variety of mechanisms involved in translation inhibition and/or RNA degradation.

In the Chlorophyta Chlamydomonas reinhardtii, however, the molecular machinery responsible for sRNA-mediated translational repression remains unclear. To address the mechanisms of translation inhibition by sRNA, we have isolated an RNAi defective mutant (Mut26), which contains a deletion of the gene encoding the homolog of CCR4 in Chlamydomonas. We investigated the expression …

Seasonal Distribution Of Sage-Grouse In Hamlin Valley, Utah And The Effect Of Fences On Grouse And Avian Predators, Heather Hedden Mcpherron

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocercus urophasianus; hereafter sage-grouse) numbers have declined throughout the western US and are considered a species of concern in most of the eleven states that are within their range. Sage-grouse habitats have been reduced by approximately 44% since European settlement of the Western United States began (Miller et al. 2011). Loss of habitat has contributed to an average decline of sage-grouse populations by 33% across the range (Connelly and Braun 1997). To expand our knowledge of this species, I monitored 16 radio-collared sage-grouse captured from four leks in Hamlin Valley, Utah, USA in 2011 and 2012 …

The Development Of A Specific Pathogen Free (Spf) Barrier Colony Of Marmosets (Callithrix Jacchus) For Aging Research, Corinna N. Ross, Steven Austad, Kathy Brasky, Celeste J. Brown, Larry J. Forney, Jonathan A. Gelfond, Robert Lanford, Arlan Richardson, Suzette D. Tardif

Biology Faculty Publications (Archived)

A specific pathogen free (SPF) barrier colony of breeding marmosets (Callithrix jacchus) was established at the Barshop Institute for Longevity and Aging Studies. Rodent and other animal models maintained as SPF barrier colonies have demonstrated improved health and lengthened lifespans enhancing the quality and repeatability of aging research. The marmosets were screened for two viruses and several bacterial pathogens prior to establishing the new SPF colony. Twelve founding animals successfully established a breeding colony with increased reproductive success, improved health parameters, and increased median lifespan when compared to a conventionally housed, open colony. The improved health and longevity of marmosets …

Ecological And Oceanographic Influences On Leatherback Turtle Behavior And Scyphozoan Jellyfish Distributions In The Gulf Of Mexico, Katrina Aleksa

Dissertations

Leatherback turtles (Dermochelys coriacea) are a wide-ranging, oceanic species that feed exclusively on gelatinous zooplankton. Leatherback have been spotted in the Gulf of Mexico (GoM) for several decades and consistently had a high level of interactions with longline fisheries. However, no quantitative studies have been performed to address the spatiotemporal distribution of these turtles in the GoM. This research determines 1) leatherback movements and high-use areas in the GoM, 2) their association with oceanographic features, 3) the distribution and density of two abundant medusae in the northern GoM and any association with biophysical parameters, and 4) the body …

Bio Buzz Newsletter, Issue 14, November 2017, Department Of Biology

Biology Buzz Newsletter

Denison University, Department of Biology newsletter for Fall 2017.

Ecological Epigenetics Of Avian Range Expansions, Holly J. Kilvitis

USF Tampa Graduate Theses and Dissertations

In light of human-mediated environmental change, a fundamental goal for biologists is to determine which phenotypic characteristics enable some individuals, populations or species to be more adept at coping with such change, while rendering others more vulnerable. Studying ongoing range expansions provide a unique opportunity to address this question by allowing documentation of how novel environments shape phenotypic variation on ecological timescales. At range-edges, individuals are exposed to strong selective pressures and population genetic challenges (e.g. bottlenecks and/or founder effects), which make genetic adaptation difficult. Nevertheless, certain species, such as the house sparrow (Passer domesticus), seem to thrive in their …

Investigation Of Moringa Oleifera Leaf Extract And Its Cancer-Selective Antiproliferative Properties, Reagen H. Welch, Ashlee H. Tietje

Journal of the South Carolina Academy of Science

Moringa oleifera is a tree native to a number of Asian, African, and Central American countries and has been used in traditional medicine for an assortment of medicinal uses for centuries. Due to bioactive compounds within Moringa leaves, it is believed that Moringa leaf extract may possess cancer-selective antiproliferative properties. Previous research has been conducted in regards to this topic, but poor experimental design due to lack of necessary controls limits the legitimacy of anticancer claims. While previous research has shown that Moringa leaf extract has the potential to kill cancer cells, the research fails to demonstrate the effects of …

Brown Marmorated Stink Bug Rearing And Nutrition, James Withers, Loren Linford

Biology Posters

The brown marmorated stink bug (BMSB), Halyomorpha halys, is an invasive insect from Asia. It has caused millions of dollars in damage to fruit, vegetable, ornamental, and field crops since its arrival in North America, and can be a severe nuisance pest when it congregates on human structures to overwinter. The BMSB caused $37 million of apple crop damage in the Mid-Atlantic Area in 2007. To study the insect, effective rearing methods are needed. Previous research (Funayama 2004 & 2006; Medal et al. 2012) showed that carrot, raw peanut and soybean increased rearing efficiency. Recent lab experience has found good …

Tracing Brain Structures Of Alkali Bees To Investigate Endocrine And Social Effects On Neural Plasticity, Justin Tirrell, Mallory A. Hagadorn, Makenna M. Johnson, Karen M. Kapheim

Biology Posters

For highly social bee species, juvenile hormone (JH) and social cues are linked to increased expression of class-based social features. However, little data has been collected on the relationship between these variables and homologous features in non-social bees. To fill this gap in data, we studied the neuroanatomical response of Nomia melanderi, a non-social bee species, to endocrine and social treatments. Since N. melanderi is a non-social bee with pre-adaptations to sociality, such as brood care and aggregative nesting habits, its responses to hormone treatment and social cues can be used to model how neural plasticity in non-social ancestors of …

Recurrent Sublethal Warming Reduces Embryonic Survival, Inhibits Juvenile Growth, And Alters Species Distribution Projections Under Climate Change, Michael A. Carlo, Eric A. Riddell, Ofir Levy, Michael W. Sears

Publications

The capacity to tolerate climate change often varies across ontogeny in organisms with complex life cycles. Recently developed species distribution models incorporate traits across life stages; however, these life-cycle models primarily evaluate effects of lethal change. Here, we examine impacts of recurrent sublethal warming on development and survival in ecological projections of climate change. We reared lizard embryos in the laboratory under temperature cycles that simulated contemporary conditions and warming scenarios. We also artificially warmed natural nests to mimic laboratory treatments. In both cases, recurrent sublethal warming decreased embryonic survival and hatchling sizes. Incorporating survivorship results into a mechanistic species …

Why Does It Take Two To Tango? Lifetime Fitness Consequences Of Parental Care In A Burying Beetle, Ashlee N. Smith, J. Curtis Creighton, Mark C. Belk

Faculty Publications

In species that require parental care, each parent can either care for their offspring or leave them in the care of the other parent. For each parent this creates three possible parental care strategies: biparental care, uniparental (male or female) care, and uniparental desertion by either the male or female. The burying beetle, Nicrophorus orbicollis, typically exhibits biparental care of offspring, and thus provides a unique system that allows us to compare the fitness benefits of these parental care strategies in an unconfounded way. In this study, we assess the lifetime fitness of biparental care, uniparental care, and uniparental …

Giant Salvinia, Salvinia Molesta (Salviniaceae): Evaluation Of Sub-Optimum Temperatures On Survival Of The Giant Salvinia Weevil, Cyrtobagous Salviniae (Coleoptera: Curculionidae) And Integration Of Management Practices With Aquatic Herbicides, Lauren W. Cozad

LSU Master's Theses

Salvinia molesta is one of the world’s worst aquatic weeds and has cost Louisiana nearly $7 million worth of damage and economic impact each year. While aquatic herbicides and the biological control agent, Cyrtobagous salviniae, are the most efficacious control methods, there are limitations with each technology. Therefore, studies were conducted to evaluate insect mortality at upper and lower lethal temperatures and investigate integrated pest management (IPM) with insects and herbicides. Three Louisiana populations of C. salviniae were tested to evaluate cold tolerance and found that at 0°C, the Bayou Nicholas population was 1.3- and 1.4-times more cold tolerant …

Phylogenetic Patterns Of Foliar Mineral Nutrient Accumulation Among Gypsophiles And Their Relatives In The Chihuahuan Desert, Clare Muller, Michael J. Moore, Zoe Feder, Helene Tiley, Rebecca E. Drenovsky

2017 Faculty Bibliography

PREMISE OF THE STUDY: Gypsum endemism in plants (gypsophily) is common on gypsum outcrops worldwide, but little is known about the functional ecology of Chihuahuan Desert gypsophiles. We investigated whether leaf chemistry of gypsophile lineages from the northern Chihuahuan Desert are similar to leaves of related nonendemic (gypsovag) species relative to their soil chemistry. We expected widely distributed gypsophiles (hypothesized to be older lineages on gypsum) would have distinct leaf chemistry from narrowly distributed, relatively younger lineages endemic to gypsum and gypsovags, reflecting adaptation to gypsum.

METHODS: We collected leaves from 23 gypsophiles and related nonendemic taxa growing on nongypsum …

Major Complications And Management Options Of Obesity, Shreja Patel, Mir Saleem

Biology Faculty Articles

Obesity is a disorder that consists of many factors, which has become a global epidemic since it is associated with various crucial diseases, such as diabetes, cardiovascular diseases, hypertension, stroke, sleep apnea, as well as other conditions. In order to manage obesity, a variety of strategies are taken into account in order to direct attention on those with current weight problems as well as those that are at a high risk of developing obesity. Therefore, obesity prevention should be focused during the early ages as there is a high risk of developing it in the later years. This article focuses …

Phosphate Starvation Induces Replacement Of Phospholipids With The Betaine Lipid Diacylglycerol-N,N,N-Trimethylhomoserine In The Human Fungal Pathogen Candida Albicans, Surabhi Naik, Rebecca E. Cahoon, Bridget Tripp, Christian Elowsky, Sophie Alvarez, Kan Liu, Chi Zhang, Wayne R. Riekhof

School of Biological Sciences: Faculty Publications

We have previously demonstrated that phosphate starvation induces replacement of phosphatidylcholine with the betaine lipid diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) in fungi. In Neurospora crassa, the BTA1 gene encodes the betaine lipid synthase, which is necessary and sufficient for DGTS synthesis. BTA1 expression and DGTS accumulation are part of the fungal phosphorus (P_i) deprivation (PHO) regulon, mediated by the NUC-1/Pho4p transcription factor. We now demonstrate that the human pathogen Candida albicans encodes a BTA1 ortholog (CaBTA1), which is activated during P_i scarcity. The CaBTA1 gene is also induced under certain biofilm-promoting conditions independent of P …

Clinical And Genome-Wide Analysis Of Cisplatin-Induced Peripheral Neuropathy In Survivors Of Adult-Onset Cancer, M. Eileen Dolan, Omar El Charif, Heather E. Wheeler, Eric R. Gamazon, Shirin Ardeshir-Rouhani-Fard, Patrick Monahan, Darren R. Feldman, Roebrt J. Hamilton, David J. Vaughn, Clair J. Beard, Chunkit Fung, Jeri Kim, Sophie D. Fossa, Daniel L. Hertz, Taisei Mushiroda, Michiaki Kubo, Lawrence H. Einhorn, Nancy J. Cox, Louis B. Travis

Biology: Faculty Publications and Other Works

No abstract provided.

Use Of Gene Editing In Usda Research, Mark R. Mclellan, Patsy Brannon, Adriana Campa, Carrie Castille, Steven Daley-Laursen, Roch Gaussoin, Govind Kannan, Mark Lawrence, Dawn Thilmany

Biology Faculty Publications and Presentations

The Science Advisory Council was established in FY2016 as a subcommittee of the National Agricultural Research, Extension, Education, and Economics (NAREEE) Advisory Board by the Chief Scientist as a result of recommendations from the 2012 report on Agricultural Preparedness by the President’s Council of Advisors on Science and Technology. The Council's charge is to provide advice and guidance, on a scientific basis, on the overall strength, practicality, and direction of agricultural research, including emerging technology and scientific issues and report any findings publicly to the NAREEE Advisory Board.

The USDA Chief Scientist first asked the Council to examine rigor and …

Seeds From Fresh Conventional Tomatoes Germinate Faster Than Dried Or Organic Seeds, Christopher G. Brown, Kautz Evan, Cameron Kilpatrick, Mary Vu, Mallory Weaver

Georgia Journal of Science

Conventionally-grown and organically-grown tomato seeds sourced from both fresh tomatoes and store-bought packages were germinated for seven days to evaluate the effect of seed source on germination rates. Seeds from fresh Roma tomatoes were prepared by allowing them to ferment in their own pulp for 24 hours, while commercially packaged dry seeds did not require any preparation. Once prepared, the seeds were spaced evenly on wet paper towels and stored in resealable plastic bags in groups of 10 for a total sample size of 100 seeds in each of four treatments. The number of germinated seeds and the length of …

Urbanization Alters Communities Of Flying Arthropods In Parks And Gardens Of A Medium-Sized City, Edward Lagucki, Justin D. Burdine, Kevin E. Mccluney

Biological Sciences Faculty Publications

Urbanization transforms undeveloped landscapes into built environments, causing changes in communities and ecological processes. Flying arthropods play important roles in these processes as pollinators, decomposers, and predators, and can be important in structuring food webs. The goal of this study was to identify associations between urbanization and the composition of communities of flying (and floating) arthropods within gardens and parks in a medium-sized mesic city. We predicted that flying arthropod abundance and diversity would respond strongly to percent impervious surface and distance to city center, measurements of urbanization. Flying arthropods were sampled from 30 gardens and parks along an urbanization …

Trees As Islands: Canopy Ant Species Richness Increases With The Size Of Liana-Free Trees In A Neotropical Forest, Benjamin J. Adams, Stefan A. Schnitzer, Stephen P. Yanoviak

Biological Sciences Faculty Research and Publications

The physical characteristics of habitats shape local community structure; a classic example is the positive relationship between the size of insular habitats and species richness. Despite the high density and proximity of tree crowns in forests, trees are insular habitats for some taxa. Specifically, crown isolation (i.e. crown shyness) prevents the movement of small cursorial animals among trees. Here, we tested the hypothesis that the species richness of ants (S_a) in individual, isolated trees embedded within tropical forest canopies increases with tree size. We predicted that this pattern disappears when trees are connected by lianas (woody vines) or …

Amblyomma Maculatum Secis Binding Protein 2 And Putative Selenoprotein P Are Indispensable For Pathogen Replication And Tick Fecundity, Khemraj Budachetri, Gary Crispell, Shahid Karim

Faculty Publications

Selenium, a vital trace element, is incorporated into selenoproteins to produce selenocysteine. Our previous studies have revealed an adaptive co-evolutionary process that has enabled the spotted fever-causing tick-borne pathogen Rickettsia parkeri to survive by manipulating an antioxidant defense system associated with selenium, which includes a full set of selenoproteins and other antioxidants in ticks. Here, we conducted a systemic investigation of SECIS binding protein 2 (SBP2) and putative selenoprotein P (SELENOP) by transcript silencing in adult female Gulf-coast ticks (Amblyomma maculatum). Knockdown of the SBP2 and SELENOP genes depleted the respective transcript levels of these tick selenogenes, and …

Connecting The Dots: Stopover Strategies Of An Intercontinental Migratory Songbird In The Context Of The Annual Cycle, Kristina Paxton, Frank R. Moore

Faculty Publications

The phases of the annual cycle for migratory species are inextricably linked. Yet, less than five percent of ecological studies examine seasonal interactions. In this study, we utilized stable hydrogen isotopes to geographically link individual black-and-white warblers (Mniotilta varia) captured during spring migration with breeding destinations to understand a migrant's stopover strategy in the context of other phases of the annual cycle. We found that stopover strategy is not only a function of a bird's current energetic state, but also the distance remaining to breeding destination and a bird's time-schedule, which has previously been linked to habitat conditions experienced …

Chlamydomonas Reinhardtii, A Model For Quorum Sensing At The Interkingdom Interface, Timothy Charles Haire

Theses and Dissertations

Microbial communities significantly impact the processes of the eukaryotic hosts they colonize. In many cases, symbiotic organisms both pathogenic and mutualistic coordinate their efforts through a process called quorum sensing (QS). QS is driven by low-molecular weight signals that regulate gene expression at threshold inducer concentrations, allowing phenotypic switching to be driven by cell density. These quorum sensing signals are subject to eukaryotic perception, and quorum associated phenotypes can directly influence the growth and development of eukaryotes. Eukaryotic perception of quorum sensing signals has been chiefly studied in plants, and to a lesser extent mammalian systems, yet no delineated model …

Itraq-Based Proteomics Analysis And Network Integration For Kernel Tissue Development In Maize, Long Zhang, Yongbin Dong, Qilei Wang, Chunguang Du, Wenwei Xiong, Xinyu Li, Sailan Zhu, Yuling Li

Department of Biology Faculty Scholarship and Creative Works

Grain weight is one of the most important yield components and a developmentally complex structure comprised of two major compartments (endosperm and pericarp) in maize (Zea mays L.), however, very little is known concerning the coordinated accumulation of the numerous proteins involved. Herein, we used isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic method to analyze the characteristics of dynamic proteomics for endosperm and pericarp during grain development. Totally, 9539 proteins were identified for both components at four development stages, among which 1401 proteins were non-redundant, 232 proteins were specific in pericarp and 153 proteins were specific in …

Food Addiction And The Effective Treatment Of Adult Food Addicts: An Integrative Review Of Literature, Sara Bourque

Grace Peterson Nursing Research Colloquium

Abstract

Background: In a survey conducted between the years of 2011 and 2014 it was suggested that 36% of adults in the U.S. were obese with over 40.2% being middle aged adults. Obesity is associated with a number of comorbid conditions including diabetes, heart attacks, and strokes. Due to this it is one of the major factors driving up our annual medical care costs. It is also one of the leading causes of death not only in the U.S. but worldwide. With one third of the adult population in the U.S. being obese food addiction may very well be a …

The Heat Shock Response And Humoral Immune Response Are Mutually Antagonistic In Honey Bees, Mia Mckinstry, Charlie Chung, Henry Truong, Brittany A. Johnston, Jonathan W. Snow

Publications and Research

The honey bee is of paramount importance to humans in both agricultural and ecological settings. Honey bee colonies have suffered from increased attrition in recent years, stemming from complex interacting stresses. Defining common cellular stress responses elicited by these stressors represents a key step in understanding potential synergies. The proteostasis network is a highly conserved network of cellular stress responses involved in maintaining the homeostasis of protein production and function. Here, we have characterized the Heat Shock Response (HSR), one branch of this network, and found that its core components are conserved. In addition, exposing bees to elevated temperatures normally …

Choline Supplementation Normalizes Fetal Adiposity And Reduces Lipogenic Gene Expression In A Mouse Model Of Maternal Obesity, Chauntelle Jack-Roberts, Yaelle Joselit, Khatia Nanobashvili, Rachel Bretter, Olga V. Malysheva, Marie A. Caudill, Anjana Saxena, Kathleen Axen, Ahmed Gomaa, Xinyin Jiang

Publications and Research

Maternal obesity increases fetal adiposity which may adversely affect metabolic health of the offspring. Choline regulates lipid metabolism and thus may influence adiposity. This study investigates the effect of maternal choline supplementation on fetal adiposity in a mouse model of maternal obesity. C57BL/6J mice were fed either a high-fat (HF) diet or a control (NF) diet and received either 25 mM choline supplemented (CS) or control untreated (CO) drinking water for 6 weeks before timed-mating and throughout gestation. At embryonic day 17.5, HF feeding led to higher (p < 0.05) percent total body fat in fetuses from the HFCO group, while the choline supplemented HFCS group did not show significant difference versus the NFCO group. Similarly, HF feeding led to higher (p < 0.05) hepatic triglyceride accumulation in the HFCO but not the HFCS fetuses. mRNA levels of lipogenic genes such as Acc1, Fads1, and Elovl5, as well as the transcription factor Srebp1c that favors lipogenesis were downregulated (p < 0.05) by maternal choline supplementation in the HFCS group, which may serve as a mechanism to reduce fat accumulation in the fetal liver during maternal HF feeding. In summary, maternal choline supplementation improves indices of fetal adiposity in obese dams at late gestation.

The Effect Of Dietary Fat On Obesity, Gene Expression, And Dna Methylation In Two Generations Of Mice, Madeline Rose Keleher

Arts & Sciences Graduate Student Theses and Dissertations

As obesity rates continue rising nationally and globally, it is crucial to understand how a high-fat diet disrupts the regulation of the genome and leads to adverse health effects. Uncovering the underlying gene expression and DNA methylation changes induced by an individual’s high-fat diet and a maternal high-fat diet can pinpoint new targets for epigenetic therapies and reveal the physiological and behavioral changes in obesity. The goal of this dissertation is to gain deeper insight into the DNA methylation and gene expression changes that occur in response to a high-fat diet.I studied the response to dietary fat within two generations …