Digital Commons Network^™

A Multi‑Disciplinary Comparison Of Great Ape Gut Microbiota In A Central African Forest And European Zoo, Victor Narat, Katherine R. Amato, Noémie Ranger, Maud Salmona, Séverine Mercier‑Delarue, Stephanie Rupp, Philippe Ambata, Richard Njouom, François Simon, Tamara Giles‑Vernick, Jérôme Legoff

Publications and Research

Comparisons of mammalian gut microbiota across different environmental conditions shed light on the diversity and composition of gut bacteriome and suggest consequences for human and animal health. Gut bacteriome comparisons across different environments diverge in their results, showing no generalizable patterns linking habitat and dietary degradation with bacterial diversity. The challenge in drawing general conclusions from such studies lies in the broad terms describing diverse habitats (“wild”, “captive”, “pristine”). We conducted 16S ribosomal RNA gene sequencing to characterize intestinal microbiota of free-ranging sympatric chimpanzees and gorillas in southeastern Cameroon and sympatric chimpanzees and gorillas in a European zoo. We conducted …

Regulation Of Mpcs And Kat8 During Adipogenesis And Nutritional Regulation, Jasmine Burrell

LSU Doctoral Dissertations

Obesity is a global epidemic characterized by an expansion of adipose tissue. Adipose tissue, composed of adipocytes and a stromal vascular fraction, is an endocrine organ that regulates whole body homeostasis. Obesity leads to the dysregulation of adipocytes and is often associated with increased susceptibility to metabolic diseases such as Type II diabetes mellitus (T2DM). Understanding the mechanisms by which adipocyte function and development are regulated is crucial. Mitochondrial pyruvate carriers (MPCs) are transmembrane proteins that transport pyruvate from the cytosol into the mitochondrial matrix to generate acetyl CoA. Although MPCs have been studied extensively, their roles in adipocytes are …

A Genomic Analysis Of Osmotolerance In Staphylococcus Aureus, Dylan Casey, Roy D. Sleator

Department of Biological Sciences Publications

A key phenotypic characteristic of the Gram-positive bacterial pathogen, Staphylococcus aureus, is its ability to grow in low aw environments. A homology transfer based approach, using the well characterised osmotic stress response systems of Bacillus subtilis and Escherichia coli, was used to identify putative osmotolerance loci in Staphylococcus aureus ST772-MRSA-V. A total of 17 distinct putative hyper and hypo-osmotic stress response systems, comprising 78 genes, were identified. The ST772-MRSA-V genome exhibits significant degeneracy in terms of the osmotic stress response; with three copies of opuD, two copies each of nhaK and mrp/mnh, and five copies of opp. Furthermore, regulation of …

The Use Of Spanish Moss As A Biological Indicator To Examine Relationships Between Metal Air Pollution, Vegetation Cover, And Environmental Equity In Tampa, Florida, Yousif Abdullah

USF Tampa Graduate Theses and Dissertations

Studies of inequality in exposure to less common air pollutants, like metals, are often limited by the costs of high spatial resolution measurements. Spanish moss (Tillandsia usneoides) is a promising bioindicator for measuring air pollution due to its lower cost, enabling capture of time-average environmental concentrations at high spatial resolution. This study had three major aims. First, I aimed to use Spanish moss as a bioindicator to characterize ambient concentrations of selected metals (Ti, Cr, Mn, Co, Ni, Cd, Hg, Pb, As, and Sb) in Tampa, Florida. My second goal was to determine the impact of vegetation cover on metals …

Microbiological, Physicochemical, And Immunological Analysis Of A Commercial Cashew Nut-Based Yogurt, Christopher P. Mattison, Kayanush Aryana, Kristen Clermont, Eric Prestenburg, Steven W. Lloyd, Casey .. Grimm, Richard L. Wasserman

Faculty Publications

Nut-based milks and yogurts are gaining popularity, but may not offer the same benefits as dairy yogurts to consumers. Cashew nuts often cause severe allergic reactions, and cashew nut allergens are stable to several types of processing. To compare its characteristics to dairy yogurt and characterize the effects of fermentation on the Ana o 1-3 cashew nut allergens, a commercial yogurt made from cashew nuts (Cashewgurt) was evaluated for microbiological, physiochemical, and immunological properties. Average counts for lactobacilli and Streptococcus thermophilus were greater than 10 million colony forming units per milliliter, indicating the capacity to provide a health benefit. Cashewgurt …

Chronic Ulcers And Malnutrition In An African Patient\, Timothy G Singer, Monica A Bray, Audrey Chan, Saki Ikeda, Brittany Walters, Maren Y Fuller, Carla Falco

Faculty, Staff and Students Publications

An 11-year-old female with a congenitally malformed left hand, sickle-cell trait, asthma, and history of appendicitis was transferred from Zambia for evaluation and treatment of widespread suppurative and ulcerative skin lesions that typically appeared following trauma to her skin. The ulcers first presented 3 years earlier but had markedly worsened in the 9 months prior to transfer, spreading circumferentially on her extremities and abdomen at the site of an appendectomy. They were painful and did not resolve with multiple courses of intravenous (IV) antibiotics and close management by a Pediatric Infectious Disease specialist working for a non-governmental organization (NGO) in …

Antibacterial Activities Of The Extracts Of Allium Sativum (Garlic) And Allium Cepa (Onion) Against Selected Pathogenic Bacteria, Sherifat O. Enejiyon, Al-Hassan A. Abdulrahman, Abdulameen S. Adedeji, Ramatu Abdulsalam, Mary U. Oyedum

Tanzania Journal of Science

Although many antibiotics have been developed against bacterial diseases, the problems of antibiotic resistance and toxicity have made the continuous search for newer drugs an existential necessity. This study determined the phytochemical components and antibacterial activities of Allium sativum and Allium cepa against Salmonella typhi, Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae and Escherichia coli. The phytochemical analysis was carried out using standard methods. The antibacterial activities of the aqueous, ethanol and acetone extracts of both plants at different concentrations were determined against all the test organisms using agar well diffusion method. All the extracts were found to contain important phytochemicals …

Hypogean Communities As Cybernetic Systems: Implications For The Evolution Of Cave Biotas, Aldemaro Romero Jr.

Publications and Research

Ramón Margalef proposed in 1968 that ecosystems could be better understood if they were viewed as cybernetic systems. I tested this hypothesis in the case of hypogean ecosystems using available pieces of evidence. I looked on how information on feedbacks, stability, succession, organization, diversity, and energy flows in the hypogean environment fit the cybernetics hypothesis. The results were that there are convincing arguments that the application of the concept of cybernetics in biospeleology can be beneficial to broadening our understanding of cave biota in terms of their structure. I also make the case that this approach can provide more clarity …

Identification Of Planktothrix (Cyanobacteria) Blooms And Effects On The Aquatic Macroinvertebrate Community In The Non-Tidal Potomac River, Usa, Joshua Henesy, Jennifer L. Wolny, John E. Mullican, Detbra S. Rosales, Joseph S. Pitula, Joseph W. Love

Virginia Journal of Science

Using transverse cross-sectional transects, a survey of 31 km of the non-tidal Potomac River was conducted from White’s Ferry, Virginia to Brunswick, Maryland, USA, between June and September in 2013 through 2015 to assess a recurring benthic cyanobacteria bloom. Abundant benthic cyanobacteria blooms were detected during the 2014 and 2015 sampling seasons and the primary taxon was identified morphologically and molecularly as Planktothrix cf. isothrix. When present, P. cf. isothrix blooms were concentrated from river center to the Maryland shoreline. This pattern was correlated with significantly greater benthic chlorophyll-a and phycocyanin concentrations. In an apparent response to …

Novel Information About The Kinetic Effects Of Equine Shoe Modifications And Kinematic Effects Of Human Digital Devices For Improved Performance In Both Species, Pengju Wang

LSU Master's Theses

Equine shoes are frequently modified to enhance traction for horses that travel on paved surfaces for work, pleasure, or entertainment. Little is known about other common shoe modifications used to enhance traction like calks, tungsten carbide granules, or plastic composition. This information is vital to shoe design to protect the safety and welfare of all service, working, and leisure horses. The objective of the first part of this thesis was to quantify the effect of shoes with and without traction adaptions on kinetic measures in non-lame, light breed horses at a trot. Kinetic data was collected with a force platform …

Analyzing Flavonoids In Chocolate-Based Beverages, Ernest Afoakwah

Kean Quest

Some amount of previous research has suggested that chocolate-based drinks are very effective post-exercise recovery beverages. The fluid, electrolyte, protein and carbohydrate content of chocolate milk has been credited with possibly providing this benefit. Antioxidants perhaps contribute to post exercise recovery. Flavonoids, also naturally occurring phytochemicals in cocoa, the chief ingredient in chocolate, may be capable of providing antioxidant benefits. Using high-performance liquid chromatography (HPLC) and colorimetry, the present research involved examining the presence of flavonoids (particularly catechin) in seven chocolate-based milk drinks, as they may serve some importance regarding post-exercise recovery. A similar protocol (which basically involved defatting samples, …

Fine-Tuning Of Alanyl-Trna Synthetase Quality Control Alleviates Global Dysregulation Of The Proteome, Paul Kelly, Arundhati Kavoor, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

One integral step in the transition from a nucleic acid encoded-genome to functional proteins is the aminoacylation of tRNA molecules. To perform this activity, aminoacyl-tRNA synthetases (aaRSs) activate free amino acids in the cell forming an aminoacyl-adenylate before transferring the amino acid on to its cognate tRNA. These newly formed aminoacyl-tRNA (aa-tRNA) can then be used by the ribosome during mRNA decoding. In Escherichia coli, there are twenty aaRSs encoded in the genome, each of which corresponds to one of the twenty proteinogenic amino acids used in translation. Given the shared chemicophysical properties of many amino acids, aaRSs have …

Artificial Nightlight Alters The Predator–Prey Dynamics Of An Apex Carnivore, Mark A. Ditmer, David C. Stoner, Clinton D. Francis, Jesse R. Barber, James D. Forster, David M. Choate, Kirsten E. Ironside, Kathleen M. Longshore, Kent R. Hersey, Randy T. Larsen, Brock R. Mcmillan, Daniel D. Olson, Alyson M. Andreasen, Jon P. Beckmann, P. Brandon Holton, Terry A. Messmer, Neil H. Carter

Life Sciences Faculty Research

Artificial nightlight is increasingly recognized as an important environmental disturbance that influences the habitats and fitness of numerous species. However, its effects on wide‐ranging vertebrates and their interactions remain unclear. Light pollution has the potential to amplify land‐use change, and as such, answering the question of how this sensory stimulant affects behavior and habitat use of species valued for their ecological roles and economic impacts is critical for conservation and land‐use planning. Here, we combined satellite‐derived estimates of light pollution, with GPS‐data from cougars Puma concolor (n = 56), mule deer Odocoileus hemionus (n = 263) and locations of cougar‐killed …

Adaptive Thermogenesis Enhances The Life-Threatening Response To Heat In Mice With An Ryr1 Mutation, Hui J Wang, Chang Seok Lee, Rachel Sue Zhen Yee, Linda Groom, Inbar Friedman, Lyle Babcock, Dimitra K Georgiou, Jin Hong, Amy D Hanna, Joseph Recio, Jong Min Choi, Ting Chang, Nadia H Agha, Jonathan Romero, Poonam Sarkar, Nicol Voermans, M Waleed Gaber, Sung Yun Jung, Matthew L Baker, Robia G Pautler, Robert T Dirksen, Sheila Riazi, Susan L Hamilton

Faculty, Staff and Students Publications

Mutations in the skeletal muscle Ca2+ release channel, the type 1 ryanodine receptor (RYR1), cause malignant hyperthermia susceptibility (MHS) and a life-threatening sensitivity to heat, which is most severe in children. Mice with an MHS-associated mutation in Ryr1 (Y524S, YS) display lethal muscle contractures in response to heat. Here we show that the heat response in the YS mice is exacerbated by brown fat adaptive thermogenesis. In addition, the YS mice have more brown adipose tissue thermogenic capacity than their littermate controls. Blood lactate levels are elevated in both heat-sensitive MHS patients with RYR1 mutations and YS mice due to …

Influence Of Characteristics Of Marine Macroalgae On Feeding Preferences Of The Milkfish (Chanos Chanos) In Peninsular Malaysia, Lim Wai Yin

Student Works (2020-2029)

Herbivores can alter several ecological mechanisms including interactions between native and non-native autotrophs that may determine invasion success of the non-native autotrophs. This study looked into the autotroph-herbivore interactions in coastal marine ecosystems of Peninsular Malaysia, mainly to determine if autotroph nutritional components and geographical differences significantly influence herbivore feeding behaviours. The relative consumption rate (RCR) of milkfish (Chanos chanos Forsskål), a generalist herbivore obtained from the Straits of Malacca was documented through feeding trials using 12 macroalgal species collected from different coastal areas of the Straits of Malacca, the Straits of Johor, and the South China Sea. The herbivore …

A Fuzzy Assessment Model For Hospitals Services Quality Based On Patient Experience, Mohamed Khodyer Alkafaji, Eman Salih Al-Shamery

Karbala International Journal of Modern Science

The patient's experience is a lens for services assessment that provide from healthcare institutions because the patient is the first and the last recipient for the service. The patient's experience carries a lot of uncertainty and an ultimate decision cannot be taken from the patient about the services, but it carries the partial truth. Many artificial intelligence technologies deal with the concept of partial truth, such as genetic algorithms and neural networks, but the fuzzy logic remains pioneering to deal with uncertainty. This paper aims to develop an assessment model by using fuzzy inference that is able to assess the …

A Systematic Mapping Study On The Risk Factors Leading To Type Ii Diabetes Mellitus, Karar N. J Musafer, Fahrul Zaman Huyop, Mufeed J Ewadh, Eko Supriyanto, Mohammad Rava

Karbala International Journal of Modern Science

Diabetes is one of the most common diseases that has had devastating effects on the general population. It is also among the most popular research trends in modern medicine. Thus, due to the complexity and desirability of this particular affliction, there is a lot of demand towards understanding this disease better, so that it can pave the way towards better solutions in combating diabetes. The aim of this review is to provide a categorization of the risk factors leading to Type II Diabetes. In order to provide a justification for the type of diabetes, an explanation is provided which covers …

Mechanistic Insights Into Strigolactone Biosynthesis, Signaling And Regulation During Plant Growth And Development, Kaiser Iqbal Wani, Andleeb Zehra, Sadaf Choudhary, M Naeem, M. Masroor A. Khan, Christian Danve Castroverde, Tariq Aftab

Biology Faculty Publications

Strigolactones (SLs) constitute a group of carotenoid-derived phytohormones with butenolide moieties. These hormones are involved in various functions, including regulation of secondary growth, shoot branching and hypocotyl elongation, and stimulation of seed germination. SLs also control hyphal branching of arbuscular mycorrhizal (AM) fungi, and mediate responses to both abiotic and biotic cues. Most of these functions stem from the interplay of SLs with other hormones, enabling plants to appropriately respond to changing environmental conditions. This dynamic interplay provides opportunities for phytohormones to modulate and augment one another. In this article, we review our current mechanistic understanding of SL biosynthesis, receptors …

Alternative Treatments To Koalas With Chlamydia, Kelsey Thornton

Fall Showcase for Research and Creative Inquiry

Koala populations have declined rapidly in the past 2 decades due to many reasons such as disease, vehicle collisions, dog attacks, urbanization and deforestation, and climate-related issues. Chlamydia is one of the leading causes for population declines in koalas. Koalas are difficult to treat for chlamydia because antibiotic treatment kills the specialized gut bacteria they have and need for degrading toxic compounds within eucalyptus leaves, their only source of food. I propose that doxycycline, a drug with a 97% cure rate and reduced dysbiosis, to be administered at higher concentrations less frequently. Additionally, I propose that koalas treated with antibiotics …

Roosting Ecology Of A Declining Population Of Northern Long-Eared Bats (Myotis Septentrionalis) In The Ozark-St. Francis National Forest Of Arkansas, Kyle Norris Edmonds

Student Theses and Dissertations

Ecological and monetary values associated with bat species are immense. Unfortunately, these benefits are at risk as disease and habitat loss have resulted in significant declines of bat populations throughout the eastern United States. Wildlife biologists and managers work tirelessly to combat the effects of these threats. Collections of species-level data during surveys provide managers with invaluable information that can be used in identification of emerging population trends. This material can then be used to generate new habitat management plans or revise existing plans in order to address the objectives of primary management goals. This thesis outlines anthropogenic factors, interspecific …

Did You Wash Your Caving Suit? Cavers’ Role In The Potential Spread Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Disease, Violeta Zhelyazkova, Antonia Hubancheva, Georgi Radoslavov, Nia Toshkova, Sebastien J. Puechmaille

International Journal of Speleology

White-Nose Disease (WND) has killed millions of hibernating bats in the US and Canada. Its causative agent, the fungus Pseudogymnoascus destructans was introduced to North America, but is native to Europe and Asia, where it is not associated with mass mortality. Although it is nearly impossible to eradicate an emerging wildlife disease, research on P. destructans spread mechanisms can aid in prevention of new introductions and development of better environmental management strategies. It is of particular importance to quantify the potential role of people visiting caves (cavers, tourists, bat researchers, etc.) whom inadvertently move P. destructans spores between sites, and …

Strategies Of Plant Biotechnology To Meet The Increasing Demand Of Food And Nutrition In India, Santanu De

Biology Faculty Articles

A groundbreaking application of biotechnology research during the recent past has been improvement of crop health and production. India being one of the most rapidly developing countries with an enormous population and remarkable biodiversity, plant biotechnology promises significant potential to contribute to characterization and conservation of the biodiversity, increasing its usefulness. However, India’s green revolution was noted to be insufficient to feed the country's teeming millions. Therefore, novel approaches in crop biotechnology had to be aimed at ensuring better productivity and quality of cultivars. This paper provides a comprehensive review of research undertaken mainly in the last couple of decades …

Are Pangolins Scapegoats Of The Covid-19 Outbreak-Cov Transmission And Pathology Evidence?, Siew Woh Choo, Jinfeng Zhou, Xuechen Tian, Siyuan Zhang, Shen Qiang, Stephen James O'Brien, Ka Yun Tan, Sara Platto, Klaus-Peter Koepfli, Agostinho Antunes, Frankie Thomas Sitam

Biology Faculty Articles

The COVID‐19 outbreak has infected over 6 million people across the world. The origin of COVID‐19 coronavirus (CoV) remains unknown, although pangolins have been suggested as potential hosts. We investigated two pangolins seized in Guangdong Province, China. Molecular screening revealed CoV in one pangolin (“Dahu”), while another (“Meidong”) was infected by Ehrlichia ruminantium. Dahu exhibited difficulty breathing, infections of lung, intestines, and nostrils, as revealed by computed tomography imaging and necropsy. Previous phylogenetic analyses showed bat coronavirus RaTG13 is closer to COVID‐19 CoV compared to pangolin coronavirus. Over 20 caregivers have had close physical contact with CoV‐positive Dahu, but …

Long‐Distance Natal Dispersal Is Relatively Frequent And Correlated With Environmental Factors In A Widespread Raptor, Hanna M. Mccaslin, T. Trevor Caughlin, Julie A. Heath

Biology Faculty Publications and Presentations

1. Dispersal is a critical process influencing population dynamics and responses to global change. Long‐distance dispersal (LDD) can be especially important for gene flow and adaptability, although little is known about the factors influencing LDD because studying large‐scale movements is challenging and LDD tends to be observed less frequently than shorter‐distance dispersal (SDD).
2. We sought to understand patterns of natal dispersal at a large scale, specifically aiming to understand the relative frequency of LDD compared to SDD and correlates of dispersal distances.
3. We used bird banding and encounter data for American kestrels (Falco sparverius) to investigate the effects of …

Quantifying Species Traits Related To Oviposition Behavior And Offspring Survival In Two Important Disease Vectors, Donald A. Yee, William C. Glasgow, Nnaemeka F. Ezeakacha

Faculty Publications

Animals with complex life cycles have traits related to oviposition and juvenile survival that can respond to environmental factors in similar or dissimilar ways. We examined the preference-performance hypothesis (PPH), which states that females lacking parental care select juvenile habitats that maximize fitness, for two ubiquitous mosquito species, Aedes albopictus and Culex quinquefasciatus. Specifically, we examined if environmental factors known to affect larval abundance patterns in the field played a role in the PPH for these species. We first identified important environmental factors from a field survey that predicted larvae across different spatial scales. We then performed two experiments, …

Investigation Of Undocumented Heart Disease In Cadaveric Donors, Crystal Danae Passburg

Doctoral Dissertations and Projects

For nearly a century, heart disease has been the leading source of death and disease in the United States, causing the deaths of hundreds of thousands of individuals every year and burdening millions more with diminished health and severe disability. However, despite the significant known prevalence of heart disease, studies suggest that many more individuals—and particularly those within the female and elderly populations—may possess undiagnosed heart disease which remains undetected even after death due to the lack of autopsy examination. In the absence of autopsy, many cases of heart disease may never be identified, resulting in a skewed understanding of …

Mtor-Initiated Metabolic Switch And Degeneration In The Retinal Pigment Epithelium, Young-Mi Go, Jing Zhang, Jolyn Fernandes, Christopher Litwin, Rui Chen, Theodore G Wensel, Dean P Jones, Jiyang Cai, Yan Chen

Faculty, Staff and Students Publications

The retinal pigment epithelium (RPE) is a particularly vulnerable tissue to age-dependent degeneration. Over the life span, the RPE develops an expanded endo-lysosomal compartment to maintain the high efficiency of phagocytosis and degradation of photoreceptor outer segments (POS) necessary for photoreceptor survival. As the assembly and activation of the mechanistic target of rapamycin complex 1 (mTORC1) occur on the lysosome surface, increased lysosome mass with aging leads to higher mTORC1 activity. The functional consequences of hyperactive mTORC1 in the RPE are unclear. In the current study, we used integrated high-resolution metabolomic and genomic approaches to examine mice with RPE-specific deletion …

Characterization Of Retinal Biomechanical Properties Using Brillouin Microscopy, Yogeshwari S Ambekar, Manmohan Singh, Giuliano Scarcelli, Elda M Rueda, Benjamin M Hall, Ross A Poché, Kirill V Larin

Faculty, Staff and Students Publications

SIGNIFICANCE: The retina is critical for vision, and several diseases may alter its biomechanical properties. However, assessing the biomechanical properties of the retina nondestructively is a challenge due to its fragile nature and location within the eye globe. Advancements in Brillouin spectroscopy have provided the means for nondestructive investigations of retina biomechanical properties.

AIM: We assessed the biomechanical properties of mouse retinas using Brillouin microscopy noninvasively and showed the potential of Brillouin microscopy to differentiate the type and layers of retinas based on stiffness.

APPROACH: We used Brillouin microscopy to quantify stiffness of fresh and paraformaldehyde (PFA)-fixed retinas. As further …

Model And Data Concur And Explain The Coexistence Of Two Very Distinct Animal Behavioral Types, Jordi Moya-Laraño, Rubén Rabaneda-Bueno, Emily Morrison, Phillip H. Crowley

Biology Faculty Publications

Behaviors may enhance fitness in some situations while being detrimental in others. Linked behaviors (behavioral syndromes) may be central to understanding the maintenance of behavioral variability in natural populations. The spillover hypothesis of premating sexual cannibalism by females explains genetically determined female aggression towards both prey and males: growth to a larger size translates into higher fecundity, but at the risk of insufficient sperm acquisition. Here, we use an individual-based model to determine the ecological scenarios under which this spillover strategy is more likely to evolve over a strategy in which females attack approaching males only once the female has …

Foliar Application Of Low Concentrations Of Titanium Dioxide And Zinc Oxide Nanoparticles To The Common Sunflower Under Field Conditions, Marek Kolenčík, Dávid Ernst, Martin Urík, Ľuba Ďurišová, Marek Bujdoš, Martin Šebesta, Edmund Dobročka, Samuel Kšiňan, Ramakanth Illa, Qian Yu, Huan Feng, Ivan Černý, Veronika Holišová, Gabriela Kratošová

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

Nano-fertilisers have only recently been introduced to intensify plant production, and there still remains inadequate scientific knowledge on their plant-related effects. This paper therefore compares the effects of two nano-fertilisers on common sunflower production under field conditions. The benefits arising from the foliar application of micronutrient-based zinc oxide fertiliser were compared with those from the titanium dioxide plant-growth enhancer. Both the zinc oxide (ZnO) and titanium dioxide (TiO₂) were delivered by foliar application in nano-size at a concentration of 2.6 mg·L⁻¹. The foliar-applied nanoparticles (NPs) had good crystallinity and a mean size distribution under 30 nm. …