Digital Commons Network^™

Linezolid Exerts Greater Bacterial Clearance But No Modification Of Host Lung Gene Expression Profiling: A Mouse Mrsa Pneumonia Model, Jiwang Chen, Gang Feng, Yang Song, Juliane B. Wardenburg, Simon Lin, Ichiro Inoshima, Michael Otto, Richard G. Wunderink

Faculty Publications – Biological Sciences

Background: Linezolid (LZD) is beneficial to patients with MRSA pneumonia, but whether and how LZD influences global host lung immune responses at the mRNA level during MRSA-mediated pneumonia is still unknown. Methods: A lethal mouse model of MRSA pneumonia mediated by USA300 was employed to study the influence of LZD on survival, while the sublethal mouse model was used to examine the effect of LZD on bacterial clearance and lung gene expression during MRSA pneumonia. LZD (100mg/kg/day, IP) was given to C57Bl6 mice for three days. On Day 1 and Day 3 post infection, bronchoalveolar lavage fluid (BALF) protein concentration …

Global Dynamics Of A Water-Borne Disease Model With Multiple Transmission Pathways, Prasanta K. Mondal, T. K. Kar

Applications and Applied Mathematics: An International Journal (AAM)

We propose and analyze a water born disease model introducing water-to-person and person-toperson transmission and saturated incidence. The disease-free equilibrium and the existence criterion of endemic equilibrium are investigated. Trans critical bifurcation at the disease-free equilibrium is obtained when the basic reproductive number is one. The local stability of both the equilibria is shown and a Lyapunov functional approach is also applied to explore the global stability of the system around the equilibria. We display the effects of pathogen contaminated water and infection through contact on the system dynamics in the absence of person-to-person contact as well as in the …

Cellular Mechanisms Of Pathogenesis Of Pseudomonas Aeruginosa, Omar Jorge Castillo

Biology Theses

Epithelial permeability is distorted by Pseudomonas aeruginosa elastase (PE) and changes in barrier function are caused by disruption of tight junction (TJ) proteins occludin, zonula occludens (ZO)-1 and alterations of the cytoskeleton. Increase in paracellular permeability is caused, in part, by activation of cellular signaling transduction pathways. The exact signaling mechanisms that lead to epithelial disruption are not yet fully understood. I sought to evaluate whether PE exposure can activate the epidermal growth factor receptor (EGFR) and whether, PE-induced TJ disruption was dependent upon activation of the extracellular signal-regulated kinases (ERK) arm of the mitogen-activated protein kinase (MAPK) pathway. My …

The Investigation Of Risk Factors For Upper Respiratory Infections Among College Students, Romie Barnes

Honors Theses

Upper respiratory tract infections and cold-like illnesses are common sources of morbidity among college students. The emphasis of hand-washing and reducing the dispersal of aerosol droplets by covering coughs and sneezes reduces the spread of the common cold. However, less emphasis is placed on other risk factors for reducing cold-like illness such as: stress level and physical activity (Doyle and Cohens 2009). An increase in stress and a decrease in physical activity are common among college students (Nichol et al 2005). For this reason, the present study analyzed the effects of stress and physical activity on the incidence of upper …

Infectious Disease: An Agent-Based Simulation., Iqra Ahmad

Undergraduate Honors Theses

One of the primary reasons for studying infectious diseases is to improve control and ultimately eradicate the infection from the population (Keeling 2008). An agent base model was designed to observe the spread of disease and the effect of vaccination using the program known as StarLogo- TNG. Experiments were designed, not having a particular disease in mind, and simulations were run to determine the effects of different variables on the vaccination process of a certain population. Goals included observation of how infections spread and how vaccination plays a role in various situations such as, root of the infection, population size, …

Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon

Masters Theses, 2010-2019

Beneficial cutaneous bacteria on amphibians can protect against the lethal fungal disease chytridiomycosis, which has decimated many amphibian species. The stability of these bacterial communities likely influences health outcomes, and is investigated here for the first time. We describe the diversity of bacteria on red-backed salamanders (Plethodon cinereus) in the wild, and the stability of these communities over time in captivity using culture-independent Illumina sequencing. In the field, there was no correlation between the diversity of salamanders’ microbial communities and the diversity of their substrates’ microbial communities. Salamanders were brought into the laboratory to test for the effect of an …

Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz

Masters Theses, 2010-2019

Amphibians worldwide are threatened by the fungal disease chytridiomycosis, caused by the skin pathogen Batrachochytrium dendrobatidis. Mutualistic skin bacteria are a critical element in amphibians’ defenses against chytridiomycosis. Probiotic bioaugmentation of beneficial, anti-Bd bacteria on amphibians is a potential conservation strategies. Outdoor experimental ponds were used to investigate transmission efficacy and persistence of the anti-Bd bacteria, Janthinobacterium lividum, on the amphibian, Notophthalmus viridescens. More specifically, this research investigated whether a short-term individual bath, environmental bioaugmentation, or both are necessary to afford transmission and persistence of J. lividum on N. viridescens. Additionally, this research investigated the effectiveness of these different probiotic …

Controlling Bacterial Persister Cells With Low Level Electric Currents, Henry Lars Peterson

Renée Crown University Honors Thesis Projects - All

Bacterial persister cells present a growing concern as they inherit the ability to tolerate high concentrations of antibiotics and repopulate after an antibiotic treatment leading to chronic diseases. Pseudomonas aeruginosa causes many human infections including skin infections and those associated with burn injuries, and implanted medical devices, and are associated with Cystic Fibrosis. Recently, the Ren Lab developed a novel approach to eliminate persister cells of P. aeruginosa, including those in biofilms, using low level electric currents. To evaluate the safety of this method and to better understand how the underlying elements, this study focused on the cytotoxicity of …

Effects Of Long-Term Exposure To Kanamycin And/Or Ampicillin On Resistance Genes On An E. Coli Plasmid, Justin W.R. Kerby Ii

Master's Theses or Doctor of Nursing Practice

Multi-drug-resistant bacteria are a major cause of hospital-acquired infections and antibiotic resistance in these organisms is often plasmid mediated, which has become a growing concern. Plasmids conferring resistance to multiple antibiotics are increasingly becoming a common source of antibiotic resistance. The behavior of these plasmids under and in the absence of selective pressure is not yet fully understood. Therefore, to determine the behavior of a multiple-resistance plasmid under selective pressure pCR 2.1-TOPO, a commercial plasmid, was inserted into an Escherichia coli host and grown in a continuous culture under four conditions: broth with 1) kanamycin alone, 2) ampicillin alone, 3) …

Antimicrobial Producing Bacteria Isolated From Petroleum-Laced Hypersaline Soil, Joanna L. Fay

Master's Theses or Doctor of Nursing Practice

Given the alternative functions of “antibiotics” as communication molecules or participants in metabolism, it seems probable that production might be influenced by factors such as nutrient availability, interactions with neighboring microbes, and/or colony or community structure and maturity. With this in mind, the present study aimed to broaden the scope of the search for novel antibiotics by experimenting with the following parameters: source of bacterial isolation, growth and assay media, and culturing techniques. Bacteria for this study were isolated from two categories of soil (petroleum-contaminated or uncontaminated) to compare diversity and antimicrobial activity. Compared to the uncontaminated soil, isolates of …

Interactions Between Protozoan Prey (Phylum Protista) And Their Mosquito Predators (Order Diptera, Family Cuclicidae) Prey Size And Predator Behavior Effects, Jeffrey Jay Skiff

Master's Theses

Mosquitoes (Diptera. Culicidae) are insects that are medically important as adults as they vector numerous diseases. Yet, understanding the ecology of the larval stage can lead to surveillance and control of adult populations. Aedes albopictus, Aedes triseriatus, Cu/ex corona/or, and Culex quinquefasciatus are four species of mosquito that co-occur in discarded automobile tires. Mosqui-to larvae feed on microorganisms (including protozoans) and particulate organic matter Most larval feeding experiments. howt:ver, have dealt exclusively with bacteria, fungi , and particulate organic matter Although bactena and fungi are important sources of nitrogen and lipids, they may be 111sufficient sources of carbon, which may …

Development Of A Genetic Marker To Differentiate Between Pulex Irritans And Pulex Simulans, Colby Russell Kearl

Undergraduate Honors Capstone Projects

Pulex irritans and Pulex simulans are zoonotic flea vectors of plague and other infectious diseases. P. irritans have historically been known to use carnivores as a host, while P. simulans primarily parasitizes omnivores. To fully understand arthropod-borne infectious disease transmissibility and potential for geographical spread, it is important to differentiate between these two flea species. Traditional taxonomy uses the flea's male morphological features to distinguish species. There are no observable morphological differences between the female P. irritans and P. simulans. Molecular markers of the internal transcribed spacer regions (ITS) have been successfully used in other insect organisms to differentiate …

The Physiological And Morphological Responses Of The Bladder Urothelium To Differential Injury, Kristina Marie Stemler

All Theses and Dissertations (ETDs)

The bladder is the site of various diseases such as urinary tract infections: UTI), bladder pain syndrome/interstitial cystitis: BPS/IC), and bladder cancer. UTIs and BPS/IC primarily afflict women and treatment costs over $5 billion and almost $200 million respectively in 2006. Quality of life in BPS/IC patients is low; comparable to individuals with end stage renal disease. Recent research has identified a role for the epithelial lining of the bladder, which is usually targeted by these afflictions, in potentially contributing to enhancing pain. The bladder epithelium also showcases a rapid ability to regenerate after injury.

Knowledge of the contribution of …

Analyzing The Population Structure Of Captive Marmosets Using Alu Retrotransposons, Megan Ranck

Honors Capstones

No abstract provided.

Assortative Mating In Ixodes Scapularis (Acari: Ixodidae) Ticks? And Ixodes Scapularis Infest Male Deer More Often Than Female Deer At Savannah River Site, (Aiken, Sc), Jenny Dickson

College of Graduate Studies: Theses & Dissertations

In this study we explored the hypothesis that the mitochondrial genetic diversity observed in Ixodes Scapularis ticks in the Southeastern United States is maintained through assortative mating. The study of 319 couples of ticks collected in copula from deer at the Savannah River Site (Aiken, SC) showed that ticks assigned to mitochondrial (12SrDNA and d-loop genes) phylogenetic monophyletic clades do not chose their mating partner based on genetic similarity or genetic diversity. The genetic composition of couples did not differ significantly from random choice. Our data indicate that if genetic diversity is maintained in this tick population, this cannot be …

Glycoconjugates Prevent Bacterial Dissemination And Cellular Death During B. Cereus/ B. Anthracis Infection, Mohamed Hassen Lahiani

Theses and Dissertations

Bacillus cereus group species including Bacillus cereus and Bacillus anthracis produce highly resistant spores. Dormant spores can survive for long period in harsh environmental conditions. Spores are engulfed by macrophages. Mammalian macrophages are the first line defenders immune cells through its phagocytic activity. Glycoconjugates (GCs) are a family of synthetic carbohydrates that possesses numerous bactericidal and immunodulatory properties. In my study, I used a multivariate approach to study the effects of GCs on B. cereus and B. anthracis Sterne phagocytosis. During in vitro study, I found that GC1 and GC3 were the best candidates that inhibited B. cereus and B. …

Rectal Transmission Of Transmitted/Founder Hiv-1 Is Efficiently Prevented By Topical 1% Tenofovir In Blt Humanized Mice, Morgan L. Chateau, Paul W. Denton, Michael D. Swanson, Ian Mcgowan, J. Victor Garcia

Biology Faculty Publications

Rectal microbicides are being developed to prevent new HIV infections in both men and women. We focused our in vivo preclinical efficacy study on rectally-applied tenofovir. BLT humanized mice (n = 43) were rectally inoculated with either the primary isolate HIV-1JRCSF or the MSM-derived transmitted/founder (T/F) virus HIV-1THRO within 30 minutes following treatment with topical 1% tenofovir or vehicle. Under our experimental conditions, in the absence of drug treatment we observed 50% and 60% rectal transmission by HIV-1JRCSF and HIV-1THRO, respectively. Topical tenofovir reduced rectal transmission to 8% (1/12; log rank p = 0.03) for HIV-1JRCSF and 0% (0/6; log …

Additional Routes To Staphylococcus Aureus Daptomycin Resistance As Revealed By Comparative Genome Sequencing, Transcriptional Profiling, And Phenotypic Studies, Yang Song, Aileen Rubio, Radheshyam K. Jayaswal, Jared A. Silverman, Brian J. Wilkinson

Faculty Publications – Biological Sciences

Daptomycin is an extensively used anti-staphylococcal agent due to the rise in methicillin-resistant Staphylococcus aureus, but the mechanism(s) of resistance is poorly understood. Comparative genome sequencing, transcriptomics, ultrastructure, and cell envelope studies were carried out on two relatively higher level (4 and 8 mu g/ml(-1)) laboratory-derived daptomycin-resistant strains (strains CB1541 and CB1540 respectively) compared to their parent strain (CB1118; MW2). Several mutations were found in the strains. Both strains had the same mutations in the two-component system genes waIK and agrA. In strain CB1540 mutations were also detected in the ribose phosphate pyrophosphokinase (prs) and polyribonucleotide nucleotidyltransferase genes (pnpA), a …

Inhibition Of Herpes Simplex Virus Type 1 With The Modified Green Tea Polyphenol Palmitoyl-Epigallocatechin Gallate, Aline De Oliveira, Sandra Adams, Lee Lee, Sean R. Murray, Stephen D. Hsu, Jeffrey R. Hammond, Douglas Dickinson, Ping Chen, Tin Chun Chu

Department of Biology Faculty Scholarship and Creative Works

Green tea polyphenol epigallocatechin gallate (EGCG) is a strong antioxidant that has previously been shown to reduce the number of plaques in HIV-infected cultured cells. Modified EGCG, palmitoyl-EGCG (p-EGCG), is of interest as a topical antiviral agent for herpes simplex virus (HSV-1) infections. This study evaluated the effect of p-EGCG on HSV-infected Vero cells. Results of cell viability and cell proliferation assays indicate that p-EGCG is not toxic to cultured Vero cells and show that modification of the green tea polyphenol epigallocatechin gallate (EGCG) with palmitate increases the effectiveness of EGCG as an antiviral agent. Furthermore, p-EGCG is a more …

13. Mathematics, University Of Central Oklahoma

Oklahoma Research Day Abstracts

No abstract provided.

Organically Grown Food Provides Health Benefits To Drosophila Melanogaster, Ria Chhabra, Santharam Kolli, Johannes H. Bauer

Biosciences Research

The ‘‘organic food’’ market is the fastest growing food sector, yet it is unclear whether organically raised food is nutritionally superior to conventionally grown food and whether consuming organic food bestows health benefits. In order to evaluate potential health benefits of organic foods, we used the well-characterized fruit fly Drosophila melanogaster as a model system. Fruit flies were raised on diets consisting of extracts of either conventionally or organically raised produce (bananas, potatoes, raisins, soy beans). Flies were then subjected to a variety of tests designed to assess overall fly health. Flies raised on diets made from organically grown produce …

Changes In Natural Killer Cell Activation And Function During Primary Hiv-1 Infection, Vivek Naranbhai, Marcus Altfeld, Salim S. Abdool Karim, Thumbi Ndung’U, Quarraisha Abdool Karim, William H. Carr

Publications and Research

Background: Recent reports suggest that Natural Killer (NK) cells may modulate pathogenesis of primary HIV-1 infection. However, HIV dysregulates NK-cell responses. We dissected this bi-directional relationship to understand how HIV impacts NK-cell responses during primary HIV-1 infection.

Methodology/Principal Findings: Paired samples from 41 high-risk, initially HIV-uninfected CAPRISA004 participants were analysed prior to HIV acquisition, and during viraemic primary HIV-1 infection. At the time of sampling post-infection five women were seronegative, 11 women were serodiscordant, and 25 women were seropositive by HIV-1 rapid immunoassay. Flow cytometry was used to measure NK and T-cell activation, NK-cell receptor expression, cytotoxic and cytokine-secretory functions, …

Long-Term Data For Endemic Frog Genera Reveal Potential Conservation Crisis In The Bale Mountains, Ethiopia, David J. Gower, Roman K. Aberra, Silvia Schwaller, Malcolm J. Largen, Ben Collen, Stephen Spawls, Michele Menegon, Breda M. Zimkus, Rafael O. De Sá, Et Al.

Biology Faculty Publications

Populations of many frogs have declined alarmingly in recent years, placing nearly one third of the > 6,000 species under threat of extinction. Declines have been attributed largely to habitat loss, environmental degradation and/or infectious diseases such as chytridiomycosis. Many frogs undergo dramatic natural population fluctuations such that long-term data are required to determine population trends without undue influence of stochastic factors. We present long-term quantitative data (individuals encountered per person hour of searching) for four monotypic frog genera endemic to an Afromontane region of exceptional importance but growing conservation concern: one endemic to the Ethiopian highlands (Spinophrynoides osgoodi) and three …

Association Of Spirochetal Infection With Morgellons Disease, Marianne J. Middelveen, Divya Burugu, Akhila Poruri, Jennie Burke, Peter J. Mayne, Eva Sapi, Douglas G. Kahn, Raphael B. Stricker

Biology and Environmental Science Faculty Publications

Morgellons disease (MD) is an emerging multisystem illness characterized by skin lesions with unusual filaments embedded in or projecting from epithelial tissue. Filament formation results from abnormal keratin and collagen expression by epithelial-based keratinocytes and fibroblasts. Recent research comparing MD to bovine digital dermatitis, an animal infectious disease with similar skin features, provided clues that spirochetal infection could play an important role in the human disease as it does in the animal illness. Based on histological staining, immunofluorescent staining, electron microscopic imaging and polymerase chain reaction, we report the detection of Borrelia spirochetes in dermatological tissue of four randomly-selected MD …

Improved Culture Conditions For The Growth And Detection Of Borrelia From Human Serum, Eva Sapi, Namrata Pabbati, Akshita Datar, Ellen M. Davies, Amy Rattelle, Bruce A. Kuo

Biology and Environmental Science Faculty Publications

In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein. The method was first optimized utilizing Borrelia laboratory strains, and later by demonstrating growth of Borrelia from sera from fifty seropositive Lyme disease patients followed by another cohort of 72 Lyme disease patients, all of whom satisfied the strict CDC surveillance case definition for Lyme disease. The procedure resulted in positive cultures in 47% at 6 days and 94% at week 16. Negative controls included 48 cases. …

Evolutionary Biology For The 21st Century, Robert Ricklefs, Jonathan Losos, Stevan Arnold, Gill Bejerano, E. Brodie Iii, David Hibbett, Hopi Hoekstra, David Mindell, Antonia Monteiro, Craig Moritz, H. Orr, Dmitri Petrov, Susanne Renner, Pamela Soltis, Thomas Turner

Biology Department Faculty Works

No abstract provided.

Nitric Oxide As A Mediator Of Hookworm Infection Ancylostoma Ceylanicum, Amanda Berndt

Undergraduate Honors Theses

Hookworm infection is a major cause of anemia, malnutrition, growth delay and cognitive defects in resource poor countries. Human and animal studies suggest that infection with these intestinal nematodes is associated with impaired cellular immunity, characterized by reduced lymphocyte proliferation in response to both parasite and heterologous antigens. In vitro studies have shown that nitric oxide (NO) is one of the leading agents causing impaired cellular responses, Spleenocytes from infected hamsters secreted more NO in culture than did those from naive animals. In order to further identify the role of NO in hookworm pathogenesis and pathology, we conducted an experiment …

Bacterial Colonization And Extinction On Marine Aggregates: Stochastic Model Of Species Presence And Abundance, Andrew M. Kramer, M. Maille Lyons, Fred C. Dobbs, John M. Drake

Integrative Biology Faculty and Staff Publications

Organic aggregates provide a favorable habitat for aquatic microbes, are efficiently filtered by shellfish, and may play a major role in the dynamics of aquatic pathogens. Quantifying this role requires understanding how pathogen abundance in the water and aggregate size interact to determine the presence and abundance of pathogen cells on individual aggregates. We build upon current understanding of the dynamics of bacteria and bacterial grazers on aggregates to develop a model for the dynamics of a bacterial pathogen species. The model accounts for the importance of stochasticity and the balance between colonization and extinction. Simulation results suggest that while …

Comparison Of Th1 Cytokines And T Cell Markers Gene Expressions Between Virulent And An Attenuated Eiav Vaccine Strain, Talia R. Henkle

Lewis Honors College Capstone Collection

The equine infectious anemia virus (EIAV) is closely related to HIV and has been used as a model to identify protective mechanisms against lentivirus infection. In horses, EIA infection progresses for about a year before infected horses manage to control virus replication. This naturally-gained protection is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. As the resolution of initial viremia correlates with the appearance of virus specific cytotoxic T lymphocytes (CTL), we believe that cellular immune responses play a key role in controlling EIAV in the horse. In …

Neutralizing Antibodies Against The Ricin Toxin Binding Subunit (Rtb), Anastasiya Yermakova

Legacy Theses & Dissertations (2009 - 2024)

Ricin is a toxin that is naturally produced by the seeds of the castor bean plant Ricinus communis, and is part of a family of A-B toxins that includes Shiga, cholera, and anthrax toxins. The toxin consists of two subunits, RTA and RTB, which are linked by a disulfide bond. RTA is an RNA N-glycosidase that selectively targets and inactivates 28S ribosomal RNA, thereby arresting protein synthesis and leading to cell death. RTB is a galactose/ N-acetylgalactosamine-specific lectin that mediates attachment, entry, and intracellular trafficking of ricin in host cells. Currently, there is no approved vaccine or therapeutics available against …