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The Resolution Phase Of Nk Cell Proliferation And Ifn Production Following Viral Infection Are Highly Regulated Processes., Leslie Abigail Fogel

Arts & Sciences Graduate Student Theses and Dissertations

In response to MCMV infection, NK cells undergo three distinct phases of proliferation: the non-specific phase mediated by pro-proliferative cytokines; the specific phase mediated by recognition of an MCMV-encoded protein by an NK cell activating receptor, Ly49H; and the resolution phase, whose mechanism is unknown. MCMV infection of RAG mice, which lack all adaptive immune cells, results in prolonged proliferation of NK cells despite similar viral titers compared to wildtype mice. Interestingly, there are different kinetics for Ly49H+ and Ly49H- NK cells. We have identified several additional markers that may distinguish NK cells that have been specifically activated through their …

Germline Defects Caused By Smed-Boule Rna-Interference Reveal That Egg Capsule Deposition Occurs Independently Of Fertilization, Ovulation, Mating, Or The Presence Of Gametes In Planarian Flatworms, Jessica Kathryne Steiner, Junichi Tasaki, Labib Rouhana

Biological Sciences Faculty Publications

Few animals are known to lay eggs in the absence of ovulation or copulation, as it is presumably energetically wasteful and subjected to negative selection. Characterization of Smed-boule, a member of the DAZ family of germline RNA-binding proteins, revealed that egg capsule (or capsule) production and deposition occurs independently of the presence of gametes in the planarian flatworm Schmidtea mediterranea. Reduction of Smed-boule expression by RNA-interference (RNAi) causes ablation of spermatogonial stem cells and the inability of ovarian germline stem cells to undergo oogenesis. Although animals subjected to Smed-boule RNAi lose their gametes and become sterile, they continue …

Investigation Of The Enviromental Factors Associated With The Temporal Abundance Of Lutzomyia Anthophora On A Ranch Near Poth, Texas, Maha Alshhrany

Theses & Dissertations

Leishmaniasis is caused by infection with protozoan parasites within the genus Leismania transmitted by the bites of female sand flies within the genus Lutzomyia in the New World. This study investigated the seasonal abundance of sand flies and evaluated the correlation of temperature with abundance data. Sand flies were collected at the Garrison ranch near Poth, Texas (28° 57’ 10” N; 98° 7’ 28” W) from May in 2014 through October, 2015. Recorded highest abundance of sand flies in 2014 was in October, 2 females Lu.anthophora and 5 males Lu.anthophora were captured producing 0.58 sand flies /trap night, while, mean …

Rac1 And Ire1 Are Required To Prevent Early Loss Of Intestinal Homeostasis In Drosophila Melanogaster, Mauricio M. Ortega

Dissertations, Masters Theses, Capstones, and Culminating Projects

No abstract available

A Novel Solution To Targeting Prosthetic Joint Infection, Christopher Chen

Theses, Dissertations and Culminating Projects

Prosthetic arthroplasty may be a life changing event for better or worse. The integrity of bone cartilages such as the lateral and medial meniscus found between the femur and tibia may degrade over time due to natural causes which may eventually result in bone on bone exposure causing excruciating pain. Prosthetics are used to replace the cartilage and bone damage using femoral and tibial component and a plastic spacer. A small percentage of all orthopedic replacement surgeries may lead to infections. These infections are caused by biofilm forming bacteria which allows bacteria to attach to abiotic surfaces and mature as …

The Effect Of Interspecies Signaling On Stenotrophomonas Maltophilia Growth., Zachary Ethan Dickinson

College of Arts & Sciences Senior Theses

Cystic fibrosis (CF) is a genetic disorder that effects over 70,000 people worldwide, and is particularly prevalent in those of Caucasian descent. CF increases susceptibility to chronic infections of the lungs and GI tract, often leading to decreased quality of life and early death to afflicted individuals. No cure is available, and so treatment is limited to treating the frequent infections that afflict CF patients. Several species of bacteria are commonly implicated in these infections, including Stenotrophomonas maltophilia. Infections of this species is of particular interest as it has been shown to be highly antibiotic resistant. This is a concern …

Bipolar Cell Dendritic Morphology Analysis In Mice With Model Retinitis Pigmentosa., Simra Ahmed

College of Arts & Sciences Senior Theses

No abstract provided.

Exploring The Mechanisms Of Action Of Antifungal Peptides Using Saccharomyces Cerevisiae., Michelle L. Mason

Biological Sciences Undergraduate Honors Theses

Candida albicans is a normal inhabitant of the skin and mucosal membranes of humans, however, in individuals with depressed immune systems or disrupted cutaneous flora, Candida can overgrow and cause serious infection. Candida infection is the fourth leading cause of nosocomial infection in the United States. These infections are often associated with longer hospital stays and higher mortality. Current drug therapies for this infection are largely ineffective due to the increased drug resistance of Candida species, and for some therapeutics, high levels of drug toxicity to humans. Histatin 5 is a naturally occurring salivary peptide that has strong antifungal properties. …

Design For Mosquito Abundance, Diversity, And Phenology Sampling Within The National Ecological Observatory Network, D. Hoekman, Y. P. Springer, C. M. Barker, R. Barrera, M. S. Blackmore, W. E. Bradshaw, D. H. Foley, H. S. Ginsberg, M. H. Hayden, C. M. Holzapfel, S. A. Juliano, L. D. Kramer, S. L. Ladeau, Todd P. Livdahl, C. G. Moore, R. S. Nasci, W. K. Reisen, H. M. Savage

Biology

The National Ecological Observatory Network (NEON) intends to monitor mosquito populations across its broad geographical range of sites because of their prevalence in food webs, sensitivity to abiotic factors, and relevance for human health. We describe the design of mosquito population sampling in the context of NEON's long-term continental scale monitoring program, emphasizing the sampling design schedule, priorities, and collection methods. Freely available NEON data and associated field and laboratory samples, will increase our understanding of how mosquito abundance, demography, diversity, and phenology are responding to land use and climate change.

Erratum: In the paper by Hoekman et al. (“Design …

The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero

Honors Theses

Needle insertion into skin for the purpose of alleviating muscle pain began with the emergence of traditional Chinese medicinal (TCM) acupuncture. “Dry needling”, a recent alternative to acupuncture, involves inserting a monofilament needle down into the deep tissue of a patient’s skin in order to reach the affected muscle. Despite its effectiveness and low risk of infection, dry needling remains questionable with respect to its safety and the lack of research to confirm the low risk of bacterial transmission via the dry needling needles. In this microbiological study, highly concentrated Tryptic Soy Agar was utilized to simulate the elasticity of …

Tick-, Mosquito-, And Rodent-Borne Parasite Sampling Designs For The National Ecological Observatory Network, Yuri P. Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, David T. Barnett, Brian F. Allan, Brian R. Amman, Christopher M. Barker, Roberto Barrera, Charles B. Beard, Lorenza Beati, Mike Begon, Mark S. Blackmore, William E. Bradshaw, Dustin Brisson, Charles H. Calisher, James E. Childs, Maria A. Diuk-Wasser, Richard J. Douglass, Rebecca J. Eisen, Desmond H. Foley, Janet E. Foley, Holly D. Gaff, Scott L. Gardner, Howard S. Ginsberg, Gregory E. Glass, Sarah A. Hamer, Mary H. Hayden, Brian Hjelle, Christina M. Holzapfel, Steven A. Juliano, Todd P. Livdahl

Biology

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

Tick-, Mosquito-, And Rodent-Borne Parasite Sampling Designs For The National Ecological Observatory Network [Special Feature: Neon Design], Yuri P. Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, David T. Barnett, Brian F. Allan, Brian R. Amman, Christopher M. Barker, Roberto Barrera, Charles B. Beard, Lorenza Beati, Mike Begon, Mark S. Blackmore, William E. Bradshaw, Dustin Brisson, Charles H. Calisher, James E. Childs, Maria A. Diuk-Wasser, Richard J. Douglass, Rebecca J. Eisen, Desmond H. Foley, Janet E. Foley, Holly D. Gaff, Scott Lyell Gardner, Howard S. Ginsberg, Gregory E. Glass, Sarah A. Hamer, Mary H. Hayden, Brian Hjelle, Christina M. Holzapfel, Steven A. Juliano, Laura D. Kramer, Amy J. Kuenzi, Shannon L. Ladeau, Todd P. Livdahl, James N. Mills, Chester G. Moore, Serge Morand, Roger S. Nasci, Nicholas H. Ogden, Richard S. Ostfeld, Robert R. Parmenter, Joseph Piesman, William K. Reisen, Harry M. Savage, Daniel E. Sonenshine, Andrea Swet, Michael J. Yabsley

Scott L. Gardner Publications

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

Ochratoxicosis In Monograstric Animals - A Review, Nafeesa Qudsia Hanif

Journal of Bioresource Management

Ochratoxin A (OTA) is a mycotoxin produced by several fungi of the genera Aspergillus and Penicillium, principally P. verrucosum in temperate climate and A. ochraceous in warm regions. In poultry feed materials, mycotoxins are found most commonly in cereals and to a lesser degree, in meals. The presence of OTA in animal feed contributes significantly to health disorders and decreased production. In addition to aflatoxins, which is an ubiquitously distributed toxin, OTA is one of the reasons for economic losses in the poultry industry due to poor performance and immunosuppression. Moreover, OTA has also been noted for the carryover …

Phenotypic And Molecular Analysis Of The Effect Of 20-Hydroxyecdysone On The Human Filarial Parasite Brugia Malayi, Amruta S. Mhashilkar, Swamy R. Adapa, Rays H.Y. Jiang, Steven A. Williams, Weam I. Zaky, Barton E. Slatko, Ashley N. Luck, Andrew Moorhead, Thomas R. Unnasch

Biological Sciences: Faculty Publications

A homologue of the ecdysone receptor has been identified and shown to be responsive to 20- hydroxyecdysone in Brugia malayi. However, the role of this master regulator of insect development has not been delineated in filarial nematodes. Gravid adult female B. malayi cultured in the presence of 20-hydroxyecdysone produced significantly more microfilariae and abortive immature progeny than control worms, implicating the ecdysone receptor in regulation of embryogenesis and microfilarial development. Transcriptome analyses identified 30 genes whose expression was significantly up-regulated in 20-hydroxyecdysone-treated parasites compared with untreated controls. Of these, 18% were identified to be regulating transcription. A comparative proteomic analysis …

Translating Ecology, Physiology, Biochemistry And Population Genetics Research To Meet The Challenge Of Tick And Tick-Borne Diseases In North America, Maria Dolores Esteve-Gassent, Ivan Castro-Arellano, Teresa Patricia Feria-Arroyo, Ramiro Patino, Andrew Y. Li, Raul F. Medina, Adalberto A. Pérez De León, Roger Iván Rodríguez-Vivas

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Emerging and re-emerging tick-borne diseases threaten public health and the wellbeing of domestic animals and wildlife globally. The adoption of an evolutionary ecology framework aimed to diminish the impact of tick-borne diseases needs to be part of strategies to protect human and animal populations. We present a review of current knowledge on the adaptation of ticks to their environment, and the impact that global change could have on their geographic distribution in North America. Environmental pressures will affect tick population genetics by selecting genotypes able to withstand new and changing environments and by altering the connectivity and isolation of several …

The Effect Of Antibiotics On Gastrointestinal Motility And Gut Microbiota, Catherine Chen

IMSAloquium Student Investigation Showcase

Antibiotics are widely used to treat infectious diseases caused by pathogenic bacteria in many disease states. While eliminating pathogens, antibiotics also affect gut commensal bacteria composition, leading to long-lasting gastrointestinal (GI) symptoms associated with GI motility problems. In this study, the mechanism by which the broad-spectrum antibiotic cefoperazone, gram-positive specific antibiotic vancomycin, and gram-negative specific antibiotic neomycin affect GI motility and gut microbial composition was examined. To assess alterations in the host after antibiotic treatment, whole gut transit time was measured by gavaging and tracing charcoal dye. Significantly delayed GI transit time in antibiotic-treated mice was observed, especially in the …

Detection Of Acinetobacter Baumanniiin Human Lice, Shamta Warang

GS4 Student Scholars Symposium

Background: Pediculus humanus humanus and Pediculus humanus capitis are bloodsucking human ectoparasites. Human body lice have been long known to vector several human pathogens, including Rickettsia prowazekii, Bartonella quintana, and Borrelia recurrentis. In contrast, human head lice are not typically associated with carriage of any pathogens, despite sporadic findings of B. quintana and Acinetobacter baumannii in lice collected from homeless people and children in different parts of the world. The purpose of this project is to assess the prevalence of A. baumannii in human head lice collected from school children from Georgia, USA. Methodology and Data: DNA of A. baumannii …

Developing Molecular Tools For Diagnosis Of Human Ehrlichiosis, Megan Mears

GS4 Student Scholars Symposium

Background: Ehrlichia chaffeensis, E. ewingii, E. muris and E. canis are known to cause febrile illnesses in humans and dogs. These pathogens are transmitted by ticks, and infection is established following invasion of the human or dog white blood cells. Ehrlichia chaffeensis infections of humans have a fatality rate up to 5%. Ehrlichia ewingii displays antigenic cross-reactivity with E. chaffeensis; however, laboratory case confirmation is difficult because E. ewingii is not cultivable so antigens and genetic data are hard to acquire. Our project will develop approaches and tools to characterize E. ewingii surface protein gene homologous to the major 120 …

Poster: Embryonic Stem Cell Research, Emillie Partridge

Best Integrated Writing

This poster presentation by Partridge provides information about stem cell research and the controversy surrounding it.

Section Abstracts: Biology

Virginia Journal of Science

Abstracts of the Biology Section for the 94th Annual Virginia Academy of Science Meeting, May 18-20, 2016, at University of Mary Washington, Fredericksburg, VA.

Clay Nanotube Composites For Antibacterial Nanostructured Coatings, Christen J. Boyer

Doctoral Dissertations

A surging demand for the development of new antimicrobial nanomaterials exists due to the frequency of medical device-associated infections and the transfer of pathogens from highly touched objects. Naturally occurring halloysite clay nanotubes (HNTs) have shown to be ideal particles for polymer reinforcement, time-release drug delivery, nano-reactor synthesis, and as substrate material for nanostructured coatings.

This research demonstrates the feasibility of a novel method for coating HNTs with metals for antibacterial applications. The first ever ability to coat HNTs through electrolysis was developed for customizable and multi-functional antibacterial nanoparticle platforms. HNTs were investigated as substrate for the deposition of copper …

Hunting, Food Subsidies, And Mesopredator Release: The Dynamics Of Crop-Raiding Baboons In A Managed Landscape, Rachel A. Taylor, Sadie J. Ryan, Justin S. Brashares, Leah R. Johnson

Integrative Biology Faculty and Staff Publications

The establishment of protected areas or parks has become an important tool for wildlife conservation. However, frequent occurrences of human-wildlife conflict at the edges of these parks can undermine their conservation goals. Many African protected areas have experienced concurrent declines of apex predators alongside increases in both baboon abundance and the density of humans living near the park boundary. Baboons then take excursions outside of the park to raid crops for food, conflicting with the human population. We model the interactions of mesopredators (baboons), apex predators, and shared prey in the park to analyze how four components affect the proportion …

School Of Biological Sciences News, 2016, School Of Biological Sciences

School of Biological Sciences News

Alumni newsletter of Illinois State University's School of Biological Sciences.

Patterns Of Olfactory Neurogenesis And Immune Response In Adult Zebrafish Following Damage Of The Olfactory Bulb, Darcy M. Trimpe

Dissertations

The adult zebrafish olfactory system, with its persistent neuronal turnover and abundant regenerative capacity, is an excellent model system for studies of adult brain plasticity. Reversible deafferentation, achieved by repeated intranasal irrigation with detergent, reduces bulbar innervation and affects bulb structure and function, while cessation of treatment permits reinnervation and recovery of bulb volume. Alterations in cell genesis may account for this phenomenon. This study focuses on adult neurogenesis in the zebrafish olfactory system following reversible deafferentation and direct olfactory bulb injury.

Potential alterations in cell proliferation, differentiation, migration, and survival in the olfactory bulb following single and repeated intranasal …

Amp Expression In Energetic Hybrid D. Melanogaster Infected With P. Rettgeri, Cassandra K. Treu, Justin Buchanan, Kristi Montooth

UCARE: Research Products

The purpose of this experiment was to investigate immune function and energy metabolism, particularly the levels of antimicrobial peptides produced in Drosophila energetically compromised genotypes. This will provide the ability to investigate energetics of immunity without changing diet. Flies were infected with the bacteria P. rettgeri and the resulting immune response was investigated. Females did not mount as effective an immune response as males. All flies exhibited decreased survivorship from infection. Control flies survived at higher levels and showed no mitochondrial:nuclear interaction.

A Hitchhiker’S Guide To Invasion Biology: Describing The Ecological Mechanisms Underlying The Range Expansions Of Two Ixodid Tick Species, Robyn M. Nadolny

Biological Sciences Theses & Dissertations

Increasing incidence of many tick-borne diseases have been linked to recent expansions of tick species distributions. Many tick species are expanding their ranges because of anthropogenic changes in the landscape, shifting climatic variables, and increasing populations of suitable host species and tick habitat. Few empirical studies have been performed, however, investigating the ecological mechanisms underlying these range expansions. Ticks are parasitic organisms that disperse across landscape by hitchhiking on hosts, but must then survive in the environment for long periods of time between bloodmeals. Two species of ixodid tick, Ixodes affinis and Amblyomma maculatum, are simultaneously expanding their ranges throughout …

An Investigation Into B Cells In Peripheral Blood And Gut Associated Lymphoid Tissues In The Red Eared Slider Turtle, Trachemys Scripta, Sarah Marie Marrochello

Theses and Dissertations

As in endotherms, immunity in ectotherms is vital to survival and contributes to the overall fitness of an organism. For reptilians, there are a number of environmental factors, such as temperature, season, and pathogen prevalence, which are known to influence both innate and adaptive immunity. Of particular interest, is the effect of these factors on B cells, a component of adaptive immunity. Reptilian B cells are reported to secrete antibodies, much like their mammalian counterparts, and undergo phagocytosis. The work reported here primarily focused on understanding the effect of environmental temperature, incubation temperature, and nesting season on B cells in …

The Signature Of God In Medicine And Microbiology An Apologetic Argument For Declarative Design In The Discoveries Of Alexander Fleming, Alan L. Gillen, Michael Cargill

Faculty Publications and Presentations

In logic and reasoning, a signature indicates the presence of an author; likewise, the characteristics of staphylococci indicate the presence of a Creator. Staphylococci and its “kind” are common bacteria, particularly in colonized people.1 Staphylococcus aureus has a complex molecular mechanism of assembling its golden pigment, staphyloxanthin. The biosynthesis of staphyloxanthin is a stellar example of irreducible complexity. Similar to staphylococci, the life and works of Alexander Fleming show the fingerprints of Providence. The so-called “serendipitous” achievements of Fleming have contributed to modern medicine, convincing Fleming and others that God was at work in his life. Fleming recognized that …

Î?- Lactam-Induced Changes In Cell Wall Composition And Autolytic Properties Of Daptomycin Resistant Staphylococcus Aureus, Michael Krzyskowski

Theses and Dissertations

Staphylococcus aureus is a major human pathogen that is well known for its ability to develop resistance to antimicrobial therapies. Recent studies have identified a combination of β-lactam antibiotic and daptomycin (DAP) to be highly effective at killing single and multi-drug-resistant S. aureus. Of particular interest is this drug combinationâ??s efficiency with which it kills strains classified as DAP-resistant methicillin-resistant Staphylococcus aureus (DAP-R MRSA). The goals of the present study were two-fold: to provide a physiological profile of a DAP-R S. aureus strain undergoing beta-lactam treatment by observing changes in autolytic rate, susceptibility to lytic enzymes, levels of peptidoglycan O-acetylation, …

The Engaged Microbiologist: Bringing The Microbiological Sciences To The K-12 Community, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

Exposing K-12 students to cutting edge science that impacts their daily lives can bring classroom lessons to life. Citizen-science projects are an excellent way to bring high-level science to the classroom and help satisfy one of the cornerstone concepts of the Next Generation Science Standards (NGSS), "engaging in practices that scientists and engineers actually use." This can be a daunting task for teachers who may lack the background or resources to integrate these projects into the classroom. This is where scientific societies such as the American Society for Microbiology (ASM) can play a critical role. ASM encourages its members to …