Digital Commons Network^™

Competitive Interactions: Spatial And Temporal Niche Partitioning Between Brook And Brown Trout In Southwest Michigan, Frederick J. Glassen

Masters Theses

Niche diet partitioning between Brook Trout and Brown Trout were studied in two southwestern Michigan, USA streams, one dominated by Brook Trout, the other with both Brook and Brown Trout. Brook Trout are endemic to eastern North America and have been introduced throughout the world for sport fishing. Brown Trout were first introduced to Michigan in 1883 using a genetic strain from Germany. Stomach contents were collected from a population of Brook Trout in the absence of Brown Trout in Frost Creek, while the other population was in potential competition with Brown Trout in Cedar Creek. Absent Brown Trout, Brook …

Carbon Sequestration In A Restored West Michigan Oak Savanna: Implications For Management Practices, Jeffrey A. Heise

Masters Theses

The savanna system is an ecosystem (i.e. a transitional ecosystem) that lies between forest and grassland ecosystems. They occur across the world in various forms, but in the North American Midwest they are specifically oak savannas: systems where the open overstory is dominated by various species of oak (Quercus spp.) and the understory consists of carbon-rich prairie grasses and forbs. This ecosystem is a highly degraded ecosystem and has lost almost 99% of its former range due to agriculture and fire suppression. Since savannas are fire-evolved systems, they are maintained by and require fire as a regular disturbance to …

Population Genomics And Conservation Of Texas Cave And Spring Salamanders (Plethodontidae: Eurycea), Andrew Brian Corbin

Biology Dissertations - Archive

Understanding species boundaries and gene flow among the cave and spring salamanders of the central Texas region (genus Eurycea) has been a challenge for many decades. Although previous research has greatly increased our understanding of these salamanders, there are still many unanswered questions involving the number of species in the group and the degree to which populations are connected. The plummeting cost of DNA sequencing has transformed the field of biology, including the field of population genetics. Before the recent advent of high-throughput sequencing, population genetic studies in Eurycea were limited to only a few genetic markers. Here, we leverage …

Taxonomy, Systematics, Biogeography, And Conservation Of Toads (Anura: Bufonidae) In The Sunda Shelf Of Indonesia, Goutam Chandra Sarker

Biology Dissertations - Archive

After Van Kampen’s 1905–1911 visit to Indonesia culminating in his Amphibians of the Indo-Australian Archipelago (1923), there has been no extensive herpetological exploration and compendium for Indonesia, particularly for Sumatra, Java, and Kalimantan. This lack of herpetological surveys has resulted in recent molecular studies from Southeast Asia lacking samples from Sumatra, Java, and Kalimantan, and subsequent underestimation of herpetofaunal diversity, inaccuracies in phylogeny and biogeography, and lack of massive conservation status assessments. This is truer for the not so aesthetically appealing and rather warty bufonid toads of Indonesia, which have received very little attention from the small scientific community who …

Analysis Of Bacterial Loads In Houston Indoor Air., Zeest Hanif Hanif

Theses (2016-Present)

House dust is a complex matrix containing organic and inorganic content with a good percentage of microorganisms. Microorganism’s growth and survival in dust depends on available moisture level. Microbial origin in dust depends on sources other than dust. On average people spend approximately 90% of their time in indoor environment. Therefore, they are continuously exposed to microbes in dust. Based on the size and volume of dust particles the microbes get access to human lungs via airways. Based on previous research; culture dependent studies indicate that Penicillium, Aspergillus, Cladosporium are in abundance is house dust along with 20 other fungal …

A Histopathological Review Of Immune Response In Largemouth Bass To Parasitic Infections Of Soft Tissues, James D. Stephenson

Theses and Dissertations

The immune response can serve as a key indicator of a fish’s overall health and the effect stressors have on the health of the fish. Anthropogenic factors can stress a fish’s immune system and inhibit immune responses. This study investigated the response of eosinophilic cells and macrophage aggregates to parasites in the livers, spleens, and gonads of Largemouth Bass, Micropterus salmoides. Largemouth Bass were sampled from three bodies of water in the Chattahoochee Valley of varying levels of urbanization. Histopathology of the aforementioned organs was conducted to observe both the parasite density and immune response. Eosinophilic cells were shown …

A Metanalysis Of Sequences Of Vascular Plants In The World’S Biodiversity Hotspots With A Special Section On Madagascar, John Michael Adrian Wojahn

Boise State University Theses and Dissertations

Humans have become a major factor in reshaping the Earth’s biosphere. One of the major effects of human changes to the environment is an increase in the rate of species extinction as compared to background rates. Biodiversity hotspots are areas whose species assemblages are very rich (50% of the world’s plants and 42% of land vertebrates) yet very threatened with extinction ( > 70% habitat destruction), and which ought to be foci for conservation efforts. The intense peril in which the flora of these endangered regions are requires an equally intense response from the scientific community. This study investigated the benefits …

Structural And Functional Insights Into Alphavirus-Host Interactions, Arthur S. Kim

Arts & Sciences Graduate Student Theses and Dissertations

Alphaviruses are arthropod-transmitted positive sense, single-stranded RNA viruses of the Togaviridae family that cause explosive, global outbreaks with significant morbidity. These viruses are classified into two groups based on genetic relatedness and clinical manifestations. Arthritogenic alphaviruses include chikungunya (CHIKV), Mayaro (MAYV), Ross River (RRV), O’nyong’nyong (ONNV) and generally cause acute and chronic musculoskeletal disease. To date, CHIKV has caused >1.8 million cases in over 40 countries. Encephalitic alphaviruses, including Eastern (EEEV), Venezuelan (VEEV), and Western (WEEV) equine encephalitis viruses, infect the central nervous system and result in encephalitic sequelae. In 2019, outbreaks of EEEV in the United States have resulted …

Examining The Efficacy Of Cover Crops As An Integrated Pest Management Tool In Organic Farms In The Lower Rio Grande Valley, Lili M. Martinez

Theses and Dissertations

The Lower Rio Grande Valley (LRGV) consists of a mix in subtropical and costal habitats, a combination that provides a favorable environment for the animals and plants that inhabit it. This is a problem for organic farmers as they refrain from using synthetic chemicals and therefore led them to seek other methods of dealing with insect herbivory. Of the many alternative methods available, we looked specifically at integrated pest management methods (IPM) to determine their efficacy in reducing insect herbivory among cash crops. Chapter II discusses an experiment performed in an organic farm in Edinburg, Texas consisting of a two-year …

Optimizing Species Selection For Forest Restoration In The Lower Rio Grande Valley, Faeqa Mohsin

Theses and Dissertations

Over the past four decades, the Lower Rio Grande Valley has been reforested with over 3.3 million native thornscrub plants in old agricultural land through transplanting native species. However, assessments of LRGV forest restoration efforts are scarce, and to address the knowledge gap, this study examined species-specific responses to restoration interventions (RIs) aimed at minimizing seedling mortality for optimizing species selection. A critical period for forest restoration in old croplands is the seedling stage, where high mortality occurs due to the interactive effects of abiotic stress, compromised soil nutrient status from intensive agriculture, and mammalian herbivory. So, we evaluated seedling …

The Long-Term Persistence And Tissue Tropism Of Brazilian Zika Virus In Monodelphis Domestica, Marisol Morales

Theses and Dissertations

Zika virus (ZIKV) rose as a major public health concern due to the congenital abnormalities of infants born to infected mothers during the 2015 Latin American outbreak. While animal models such as transgenic mice and nonhuman primates are used to study ZIKV pathogenesis, they display various limitations. Additionally, the persistence and tissue distribution of long-term ZIKV infection remains unknown. We describe the laboratory opossum, Monodelphis domestica, as a potential new model for ZIKV pathogenesis research to address these issues. We utilized immunohistochemistry, RT-PCR, and ELISA to show that: infant animals inoculated intracerebrally were susceptible to infection, ZIKV infection persisted …

The Effects Of Elevation On Foraging Behavior Of Bats In Southern Appalachia, Victoria Long

Electronic Theses and Dissertations

There are limited studies on bat activity in higher elevations in the Appalachian region. Levels of bat activity were compared in south central Appalachia at low (< 914.4 m) and high (> 1, 524 m) elevations in open, forest edge, and riparian habitats. Additionally, habitat suitability was modeled for a common species, big brown bat (Eptesicus fuscus). The study started May 27^th 2019, and sites were monitored biweekly until October 2^nd 2019. Six species and one genus were recorded during the study. Species from the Myotis genus were grouped together because of similar call characteristics. Results show that species were significantly more …

Embryonic Exposure To The Synthetic-Progesterone Levonorgestrel (Lng) Results In Hyperactive Behavior In Zebrafish (Danio Rerio), Austin D. Fuentes

Theses and Dissertations

Exposure to endocrine-disrupting chemicals has been associated with an increased risk of anxiety-like behavior at various life stages. Levonorgestrel (LNG), a synthetic contraceptive, could potentially alter steroidal regulated brain development based on the findings of this thesis. In-silico measurements were used for comparing LNG, which had been found in surface water, to chemical databases to predict the bioavailability, half-life, and estrogen binding potential. Zebrafish (Danio rerio) for illuminating the effects of LNG exposure during early zebrafish embryonic development, similar to fetal development, on behavior was investigated. Our data suggests exposure in a dose-dependent manner of LNG can potentially …

A Novel Quantitative Real-Time Pcr Diagnostic Assay For Fecal And Nasal Swab Detection Of An Otariid Lungworm, Parafilaroides Decorus, Kalani M. Williams, M. K. Fessler, R. A. Bloomfield, William D. Sandke, Clara R. Malekshahi, Caroline D. Keroack, Pádraig J. Duignan, Samantha D. Torquato, Steven A. Williams

Biological Sciences: Faculty Publications

Parafilaroides decorus, also known as sea lion lungworm, is a metastrongyloid nematode that infects otariid hosts, such as the charismatic California sea lion, Zalophus californianus. P. decorus causes bronchointerstitial pneumonia, respiratory distress, reduced ability to swim, dive and hunt and as a result, increased mortality particularly in young animals. Respiratory disease is a leading cause of stranding and admission to rehabilitation centers on the Pacific coast. Low-coverage genomic sequencing of four P. decorus individuals analyzed through Galaxy's RepeatExplorer identified a novel repeat DNA family we employed to design a sensitive quantitative PCR (qPCR) assay for diagnosing infections from fecal or …

Investigating Nest Box Utilization By Bumble Bees And Reproductive Development Of Male Bumble Bees, James Daniel Herndon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bumble bees (Bombus Latrielle) are a wide-spread group of pollinating insects that are important species to conserve across many environments to ensure both ecological and economic resiliency because they pollinate important agricultural, horticultural, and wild flora. Surprisingly, fundamental questions still remain about this important charismatic group of pollinators. The investigations in this thesis are but two of many topics that require further research. The topics investigated are understanding bumble bee nest site preferences and reproductive development of both sexes of bumble bees. The first experiment (Chapter 2) investigates whether nest boxes elevated off of the ground and attached to …

A Phylogenetic Assembly And Positive Selection Analysis Of The Dmp1 Gene In Aves, Testudines And Crocodilia, Nicholas Michael Morelli

Theses, Dissertations and Culminating Projects

The DMP1 gene codes for proteins that play a role in the calcification of hard tissues, including dentin, in vertebrates. Deleterious mutations in the gene can cause genetic disorders such as rickets in humans. The gene function, however, has only been investigated widely within mammalian lineages, and has not been thoroughly researched within sauropsids (reptiles and birds). Birds are edentulous (toothless) and have a unique bone structure that allows a majority of species to withstand the rigors of flight. In contrast, their closest living relatives, the crocodilians, are polydontous and possess dense skeletons as an adaptation to their semi-aquatic lifestyle. …

Assessing The Impact Of Restored Wetlands On Bat Foraging Activity Over Nearby Farmland, Philip Allagas

Electronic Theses and Dissertations

Up to 87% of the world’s wetlands have been destroyed, considerably reducing ecosystem services these wetlands once provided. More recently, many wetlands are being restored in an attempt to regain their ecosystem service. This study seeks to determine the effects of restored wetlands on local bat habitat use. Bat activity was found to be significantly higher around the wetlands when compared to distant grassy fields; however, no significant difference was found among the restored wetlands and a remote cattle farm containing multiple water features. Geospatial models of bat distribution and bat foraging were produced using machine learning that showed higher …

Bdnf-Related Gene Expression Of Laser Capture Microdissected Glutamate Neurons From The Anterior Cingulate Cortex In Mouse Models Of Autism Spectrum Disorder, Misty Owens

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) is a neurodevelopmental disorder affecting social behaviors. ASD affects 1 in 59 children with males affected more frequently. ASD is postulated to result from excitatory and inhibitory neurotransmission imbalances. Brain-derived neurotrophic factor (BDNF) signaling affects ASD by influencing synaptogenesis, plasticity, and survival. Studying early in-utero neuropathological changes within ASD requires the use of animal models. Expression of BDNF-associated genes were analyzed within laser capture microdissected pyramidal neurons from the anterior cingulate cortex of male and female BTBR and valproic acid mouse models. No expression differences were found in any gene comparing the three groups. Gender comparisons …

Natural By Design, Craig Steele

Journal of Humanistic Mathematics

I’m a professor in the Department of Biology and Health Sciences at Edinboro University, in Edinboro, Pennsylvania, a small, comprehensive liberal arts institution within the Pennsylvania State System of Higher Education. My major teaching duties involve environmental biology, zoology, and ichthyology. I emphasize to my students how mathematics underlies the natural world of plants and animals, pointing out to them how many of “our” most amazing engineering and constructional achievements are copied from nature (from geodesic domes to the fusiform bows of modern commercial ships), as well as how plant and animal physiology and animal behavior (of individuals and of …

Spatial Ecology And Resource Selection Of Bighorn Sheep (Ovis Canadensis) Ewes In A Prairie Badlands Population, Erin Wood

School of Natural Resources: Dissertations, Theses, and Student Research

The basic spatial ecology and habitat relationships of female bighorn sheep in Nebraska are poorly understood. Establishing seasonal patterns of space use and resource selection for this population at the margin of their historical and current range addresses a key knowledge gap and provides important baseline information for ongoing conservation efforts in Nebraska. We deployed GPS radio-collars on 56 adult ewes in western Nebraska to quantify seasonal space use, movements, and resource selection of ewes. To investigate spatial ecology, we quantified movements of ewes and the factors that influence home range size, seasonal use, and spatial stability across seasons. Home …

Assessing Well-Being Of Asian Elephants (Elephas Maximus) Through Major Events And Transitions, With Development And Aging, And Husbandry Changes, Sharon Stuart Glaeser

Dissertations and Theses

In this body of research, reproductive and adrenal hormones and behavior were used to evaluate individual and group responses to physiological, social, and environmental changes in zoo-housed Asian elephants, considering factors of sex, age, and life stage. Animals experience physiological, social, and environmental changes as part of their natural history and individual life experience. Measures of both positive and negative states are needed to assess the impact of these changes at the individual and group level. Such measures can help us better understand how animals cope with a changing environment, and can help inform management decisions. Through longitudinal analyses of …

Rapid: Covid-19 Induced Cessation Of Ecotourism And Supplemental Feeding: Implications For Wildlife Physiology, Reproduction, And The Microbiome, Susannah S. French

Funded Research Records

No abstract provided.

Energy Stores And Life-History Transitions In Red-Sided Garter Snakes (Thamnophis Sirtalis Parietalis), Rachel Catharine Wilson

Dissertations and Theses

All organisms must prioritize investment in either reproductive or self-maintenance activities. Despite this established paradigm, our understanding of how organisms choose to prioritize certain behaviors and physiologies over others remains limited. It is likely that an organism's energy status not only influences variation in reproductive effort, but also transitions to and from reproductive activities. My dissertation aims to investigate how energy metrics (body condition index, adipocyte follicle size, and liver glycogen) relate to reproduction and associated life-history stages in red-sided garter snakes (Thamnophis sirtalis parietalis). Chapter two examines if energy metrics differ with migratory status and sex in …

Individual Learning Phenotypes Drive Collective Behavior, Chelsea N. Cook, Natalie J. Lemanski, Thiago Mosqueiro, Cahit Ozturk, Jürgen Gadau, Noa Pinter-Wollman, Brian H. Smith

Biological Sciences Faculty Research and Publications

Individual differences in learning can influence how animals respond to and communicate about their environment, which may nonlinearly shape how a social group accomplishes a collective task. There are few empirical examples of how differences in collective dynamics emerge from variation among individuals in cognition. Here, we use a naturally variable and heritable learning behavior called latent inhibition (LI) to show that interactions among individuals that differ in this cognitive ability drive collective foraging behavior in honey bee colonies. We artificially selected two distinct phenotypes: high-LI bees that ignore previously familiar stimuli in favor of novel ones and low-LI bees …

Growth And Immune Response Of Pond-Reared Giant Freshwater Prawn Macrobrachium Osenbergii Post Larvae Fed Diets Containing Chlorella Vulgaris, Gian Carlo F. Maliwat, Stephanie F. Velasquez, Sheila Marie D. Buluran, Melchor Tayamen, Janice A. Ragaza

Biology Faculty Publications

A 70-day feeding trial was conducted to evaluate the efficacy of Chlorella vulgaris supplementation in diets of giant freshwater prawn (Macrobrachium rosenbergii) post larvae. Resistance of the prawns against Aeromonas hydrophila and several immune parameters (total hemocyte count and prophenoloxidase activity) were also assessed in the study. Iso-nitrogenous, iso-lipidic, and iso-caloric prawn feeds were prepared utilizing a fishmeal based positive control diet (F0) and four dietary treatments with C. vulgaris inclusion levels of 2 (F2), 4 (F4), 6 (F6), and 8 (F8) %. Post larvae of M. rosenbergii were stocked randomly (mean initial body weight of 0.39 ± …

Sensing Malignancy: Expanding Upon The Nematode Scent Detection Test, Morgan Alexandra Thompson

Dissertations and Theses

With the growing prevalence of cancer worldwide, research has increasingly focused on methods of early detection since the early detection of cancer can dramatically improve health outcomes for affected individuals. Ideally, early detection systems would be highly reliable yet cost-effective and easy to use. Toward development of such a system, Hirotsu et al. (2015), developed a "Nematode Scent Detection Test" (NSDT) that capitalizes upon the attraction of Caenorhabditis elegans nematodes to various tissue samples from cancer patients to detect multiple types and stages of cancer. However, it is currently unknown which G-protein coupled receptors (GPCRs), and/or ligands interacting with theses …

Insights From Population Genomics To Enhance And Sustain Biological Control Of Insect Pests, Arun Sethuraman, Fredric J. Janzen, David W. Weisrock, John J. Obrycki

Biology Faculty Publications

Biological control—the use of organisms (e.g., nematodes, arthropods, bacteria, fungi, viruses) for the suppression of insect pest species—is a well-established, ecologically sound and economically profitable tactic for crop protection. This approach has served as a sustainable solution for many insect pest problems for over a century in North America. However, all pest management tactics have associated risks. Specifically, the ecological non-target effects of biological control have been examined in numerous systems. In contrast, the need to understand the short- and long-term evolutionary consequences of human-mediated manipulation of biological control organisms for importation, augmentation and conservation biological control has only recently …

Wild Mice With Different Social Network Sizes Vary In Brain Gene Expression, Patricia C. Lopes, Barbara König

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Background

Appropriate social interactions influence animal fitness by impacting several processes, such as mating, territory defense, and offspring care. Many studies shedding light on the neurobiological underpinnings of social behavior have focused on nonapeptides (vasopressin, oxytocin, and homologues) and on sexual or parent-offspring interactions. Furthermore, animals have been studied under artificial laboratory conditions, where the consequences of behavioral responses may not be as critical as when expressed under natural environments, therefore obscuring certain physiological responses. We used automated recording of social interactions of wild house mice outside of the breeding season to detect individuals at both tails of a distribution …

Cbd & Ethanol Disrupt Early Embryonic Development, Janasha Goffigan-Holmes

Master of Science in Integrative Biology Theses

The heavily conserved neural circuits that govern the vertebrate reward system, receive sensory stimuli and produce appropriate, self-preserving responses that correspond to experience-based learning and are driven by the communication of dopamine. Many of these circuits lie within the endocannabinoid system (ECS). At the most basic level, the ECS drives homeostatic behaviors, such as seeking nutrients, avoiding danger, and reproductive function. Unfortunately, this system can be hijacked by psychoactive drugs which ultimately alter the expression of dopamine. Cannabis sativa has two main active ingredients, THC and CBD, which modify the ECS receptor’s ability to bind to hormones and neurotransmitters such …

Multivariate Analysis Of Open Field Exploration Identifies Latent Spatial And Social Behavioral Axes In Domestic Dogs, Budhaditya Chowdhury, Moira Van Staaden, Robert Huber

Biological Sciences Faculty Publications

Recent methodological advances in studying large scale animal movements have let researchers gather rich datasets from behaving animals. Often collected in small sample sizes due to logistical constraints, these datasets are however, ideal for multivariate explorations into behavioral complexity. In behavioral studies of domestic dogs, although automated data loggers have recently seen increasing use, a comprehensive framework to identify complex behavioral axes is lacking. Dog behavioral studies frequently rely on subjective ratings, despite demonstrable evidence that these are insufficient for identifying behavioral variables. Taking advantage of dogs' innate running abilities and readily available GPS data loggers, we extracted latitude-longitude coordinates …