Digital Commons Network^™

Elucidation Of The Interaction Between Hemorphins And Targets In The Renin-Angiotensin System, Amie Jobe

Theses

Hemorphins, short bioactive peptides produced by the enzymatic cleavage of the hemoglobin β-chain, exhibit anti-hypertensive effects via the inhibition of the angiotensin-1 converting enzyme (ACE1), a key component of the renin-angiotensin system (RAS) that governs blood pressure regulation. ACE1 and its homolog ACE2, which is also involved in the RAS, share significant similarity in their catalytic domains. The main objective of this work was to identify and compare the molecular mechanisms that underlie the interaction of hemorphins of camels and that of other mammals with ACE1 and ACE2. In silico docking and molecular dynamics simulations were employed, and in vitro …

Foxo1 Represses Sprouty 2 And Sprouty 4 Expression To Promote Arterial Specification And Vascular Remodeling In The Mouse Yolk Sac, Nanbing Li-Villarreal, Rebecca Lee Yean Wong, Monica D Garcia, Ryan S Udan, Ross A Poché, Tara L Rasmussen, Alexander M Rhyner, Joshua D Wythe, Mary E Dickinson

Faculty, Staff and Students Publications

Establishing a functional circulatory system is required for post-implantation development during murine embryogenesis. Previous studies in loss-of-function mouse models showed that FOXO1, a Forkhead family transcription factor, is required for yolk sac (YS) vascular remodeling and survival beyond embryonic day (E) 11. Here, we demonstrate that at E8.25, loss of Foxo1 in Tie2-cre expressing cells resulted in increased sprouty 2 (Spry2) and Spry4 expression, reduced arterial gene expression and reduced Kdr (also known as Vegfr2 and Flk1) transcripts without affecting overall endothelial cell identity, survival or proliferation. Using a Dll4-BAC-nlacZ reporter line, we found that one of the earliest expressed …

Targeting Calcium-Mediated Inter-Organellar Crosstalk In Cardiac Diseases, Mohit M Hulsurkar, Satadru K Lahiri, Jason Karch, Meng C Wang, Xander H T Wehrens

Faculty, Staff and Students Publications

INTRODUCTION: Abnormal calcium signaling between organelles such as the sarcoplasmic reticulum (SR), mitochondria and lysosomes is a key feature of heart diseases. Calcium serves as a secondary messenger mediating inter-organellar crosstalk, essential for maintaining the cardiomyocyte function.

AREAS COVERED: This article examines the available literature related to calcium channels and transporters involved in inter-organellar calcium signaling. The SR calcium-release channels ryanodine receptor type-2 (RyR2) and inositol 1,4,5-trisphosphate receptor (IP

EXPERT OPINION: Enhanced SR calcium release via RyR2 and reduced SR reuptake via SERCA2a, increased VDAC and MCUC-mediated calcium uptake into mitochondria, and enhanced lysosomal calcium-release via lysosomal TPC and TRPML …

Intestinal Deletion Of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Promotes Expansion Of The Resident Stem Cell Compartment, Alexandria M Doerfler, Jun Han, Kelsey E Jarrett, Li Tang, Antrix Jain, Alexander Saltzman, Marco De Giorgi, Marcel Chuecos, Ayrea E Hurley, Ang Li, Pauline Morand, Claudia Ayala, David R Goodlett, Anna Malovannaya, James F Martin, Thomas Q De Aguiar Vallim, Noah Shroyer, William R Lagor

Faculty, Staff and Students Publications

BACKGROUND: The intestine occupies the critical interface between cholesterol absorption and excretion. Surprisingly little is known about the role of de novo cholesterol synthesis in this organ, and its relationship to whole body cholesterol homeostasis. Here, we investigate the physiological importance of this pathway through genetic deletion of the rate-limiting enzyme.

METHODS: Mice lacking 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Hmgcr) in intestinal villus and crypt epithelial cells were generated using a Villin-Cre transgene. Plasma lipids, intestinal morphology, mevalonate pathway metabolites, and gene expression were analyzed.

RESULTS: Mice with intestine-specific loss of Hmgcr were markedly smaller at birth, but gain …

Bistability And Switching Behavior In Moving Animal Groups, Daniel Strömbom, Stephanie Nickerson, Catherine Futterman, Alyssa Difazio, Cameron Costello, Kolbjørn Tunstrøm

Northeast Journal of Complex Systems (NEJCS)

Moving animal groups such as schools of fish and flocks of birds frequently switch between different group structures. Standard models of collective motion have been used successfully to explain how stable groups form via local interactions between individuals, but they are typically unable to produce groups that exhibit spontaneous switching. We are only aware of one model, constructed for barred flagtail fish that are known to rely on alignment and attraction to organize their collective motion, that has been shown to generate this type of behavior in 2D (or 3D). Interestingly, another species of fish, golden shiners, do exhibit switching …

Mammals Adjust Diel Activity Across Gradients Of Urbanization, Travis Gallo, Mason Fidino, Brian Gerber, Adam A. Ahlers, Julia L. Angstmann, Max Amaya, Amy L. Concilio, David Drake, Danielle Gray, Elizabeth W. Lehrer, Maureen H. Murray, Travis J. Ryan, Colleen Cassady St. Clair, Carmen M. Salsbury, Heather A. Sanders, Theodore Stankowich, Jacque Williamson, J. Amy Belaire, Kelly Simon, Seth B. Mangle

Scholarship and Professional Work - LAS

Time is a fundamental component of ecological processes. How animal behavior changes over time has been explored through well-known ecological theories like niche partitioning and predator–prey dynamics. Yet, changes in animal behavior within the shorter 24-hr light–dark cycle have largely gone unstudied. Understanding if an animal can adjust their temporal activity to mitigate or adapt to environmental change has become a recent topic of discussion and is important for effective wildlife management and conservation. While spatial habitat is a fundamental consideration in wildlife management and conservation, temporal habitat is often ignored. We formulated a temporal resource selection model to quantify …

Origin And Epigenetic Regulation Of Cutaneous T Cell Lymphoma, Carly M. Harro

USF Tampa Graduate Theses and Dissertations

The pathogenesis of Cutaneous T cell lymphoma (CTCL) remains unclear. Using conditional knockout mice, we found that ablation of the genomic organizer Special AT-rich sequence-binding protein-1 (Satb1) caused malignant transformation of mature, skin-homing, Notch-activated CD4+ and CD8+ T cells into progressively fatal lymphoma. Mechanistically, Satb1 restrained Stat5 phosphorylation and the expression of skin-homing chemokine receptors in mature T cells. H3K27 and H3K9 trimethylation occluded the SATB1 promoter in Sézary cells, while inhibition of SUV39H1/2 methyltransferases (unlike EZH2 inhibition), restored protective SATB1 expression and selectively abrogated the growth of primary Sézary cells more effectively than romidepsin. Additionally, using single-cell RNA/TCR sequencing …

Using Constructed Wetlands To Remove Pathogenic Parasites And Fecal Coliforms From Wastewater In Tanzania, Abdallah Zacharia, Wajihu Ahmada, Anne H. Outwater, Billy Ngasala, Rob Van Deun

Tanzania Journal of Science

Wastewater treatment is a widely used health protection measure that can be applied to control the transmission of wastewater-related infectious diseases to communities exposed to wastewater. This study determined the efficiency of three full-scale constructed wetlands (CWs) in removing pathogenic parasites and fecal coliforms (FC) bacteria from wastewater. Wastewater samples were collected from three CW systems located in the Dar es Salaam and Iringa regions of Tanzania. The modified Bailenger and modified Ziehl-Neelsen stain techniques were used to detect and quantity parasites. The membrane filtration method was used to detect and quantify FC. Data were analysed using IBM SPSS version …

Socio-Economic Factors Of Environmental Problems, Manzura Kamalova, D. Shakenov

Karakalpak Scientific Journal

People consciously and intensively rebuild their nature according to their own needs, creating rural and urban areas in which they live. People care about the quality of life in the context of the unity of nature and culture. Social is a relatively new discipline that deals with urban space and environmental issues. One way to imagine what is beneficial to people is to find out what is economically best for the person. The article examines the economic factors that form the attitude towards social ecology in Kazakhstan. The article concludes that society should cooperate to achieve common things, both for …

Ohio Anuran Gut Microbiomes, Serena Hoffman

Undergraduate Honors Thesis Projects

Recent studies focusing on the skin and gut microbiome of vertebrates have found how important microbiomes are to maintaining host health. Understanding the role the gut microbiome plays to host health is of great importance for conservation. Less is known about the gut microbiome than the skin microbiome for amphibians. Literature is especially lacking for local Ohio anuran species. The purpose of this study was to establish the baseline composition of the gut microbiome for local Ohio anurans and to determine whether host species or habitat is the best predictor of gut microbiome composition. Tadpoles were collected from seven different …

Aging-Associated Changes In Motor Function Are Ovarian Somatic Tissue-Dependent, But Berm Cell And Estradiol Independent In Post-Reproductive Female Mice Exposed To Young Ovarian Tissue, Tracy L. Habermehl, Kaden B. Underwood, Kevin D. Welch, Steven P. Gawrys, Kate C. Parkinson, Augusto Schneider, Michal M. Masternak, Jeffrey Mason

Center for Integrated Biosystems Publications

A critical mediator of evolution is natural selection, which operates by the divergent reproductive success of individuals and results in conformity of an organism with its environment. Reproductive function has evolved to support germline transmission. In mammalian ovaries, this requires healthy, active gonad function, and follicle development. However, healthy follicles do not contribute to germline transmission in a dead animal. Therefore, support of the health and survival of the organism, in addition to fertility, must be considered as an integral part of reproductive function. Reproductive and chronological aging both impose a burden on health and increase disease rates. Tremors are …

Oleic Acid Is An Endogenous Ligand Of Tlx/Nr2e1 That Triggers Hippocampal Neurogenesis, Prasanna Kandel, Fatih Semerci, Rachana Mishra, William Choi, Aleksandar Bajic, Dodge Baluya, Lihua Ma, Kevin Chen, Austin C Cao, Tipwarin Phongmekhin, Nick Matinyan, Alba Jiménez-Panizo, Srinivas Chamakuri, Idris O Raji, Lyra Chang, Pablo Fuentes-Prior, Kevin R Mackenzie, Caroline L Benn, Eva Estébanez-Perpiñá, Koen Venken, David D Moore, Damian W Young, Mirjana Maletic-Savatic

Faculty, Staff and Students Publications

Neural stem cells, the source of newborn neurons in the adult hippocampus, are intimately involved in learning and memory, mood, and stress response. Despite considerable progress in understanding the biology of neural stem cells and neurogenesis, regulating the neural stem cell population precisely has remained elusive because we have lacked the specific targets to stimulate their proliferation and neurogenesis. The orphan nuclear receptor TLX/NR2E1 governs neural stem and progenitor cell self-renewal and proliferation, but the precise mechanism by which it accomplishes this is not well understood because its endogenous ligand is not known. Here, we identify oleic acid (18:1ω9 monounsaturated …

Ocean Quahog (Arctica Islandica) Population Dynamics: Sex-Based Demographics And Regional Comparisons In The Northwest Atlantic, Kathleen M. Hemeon

Dissertations

Arctica islandica (ocean quahog) is the longest-lived bivalve on Earth. Individuals on the deep continental shelf of the Mid-Atlantic (US) can survive for centuries, and when found in the colder, boreal waters of Iceland, ages over 500 years can be reached. The ocean quahog is important in the US, yet very little is known about the resiliency of the ocean quahog stock to fishing activity, and ocean quahog recruitment patterns over time. To quantify and constrain age-reader error prior to age analysis, a triple-method error protocol was developed for A. islandica that included age-reader bias, precision, and error frequency. The …

High-Frequency Accelerometer Recording Of Key Predatory Behaviors In Vipers: Validation And Case Study With Timber Rattlesnakes (Crotalus Horridus), Morgan L. Thompson, Richard H. Adams, Anna Tipton, Dominic L. Desantis

Graduate Research Showcase

High-frequency accelerometer recording of key predatory behaviors in vipers: validation and case study with Timber Rattlesnakes (Crotalus horridus)

Morgan Thompson, Richard H. Adams, Anna F. Tipton, and Dominic L. DeSantis

Tri-axial accelerometers (ACTs) are becoming increasingly common in studies of animal behavior wherein direct observation of subjects in nature is constrained or impossible. ACTs are small (< 1 g) piezo-electric (spring-like) sensors that measure three-dimensional acceleration (upward, downward, and side-to-side) derived from subject motion. When leveraged with advanced machine learning techniques, these data can enable precise automated classification of a wide range of movement-mediated behaviors. Until recently, ACTs were largely reserved for larger-bodied organisms or those most amenable to the temporary external attachment of devices. Ongoing ACT miniaturization has now expanded the breadth of organisms amenable to these methods. This project aims to expand on a recently developed framework for ACT monitoring in wild-ranging snakes, a group that has been mostly overlooked in biologging applications. We are currently conducting extensive captive validation trials for robust model training and testing to enable classification of predatory behaviors, including striking and ingestion of prey items, in Timber Rattlesnakes (Crotalus horridus). Following captive validation, we will translate this method to the field with a population of C. horridus in the lower Piedmont of middle Georgia to evaluate the efficacy of externally attached ACTs for remote and continuous monitoring of …

Endogenous Cftr Expression In Human Epithelial Cell Lines, Zithlaly Amezquita

Undergraduate Research Conference

CFTR (Cystic Fibrosis Transmembrane-conductance Regulator) is a plasma membrane protein that functions as a chloride ion channel on many epithelial cells. There are over 1000 mutations that affect the function of this protein; however, the most common mutation is DF508. CFTR mutations target many secretory organs like pancreas and lungs causing the genetic disease cystic fibrosis (CF), common among Caucasians of northern European origin. Detection of endogenous CFTR expression is possible by a difficult and costly immunoprecipitation method but not by the most used western blotting method. Therefore, the goal of this project was to detect the endogenous CFTR expression …

Size Structure And Intraguild Interactions Between Two Ambystoma Species, Gwyneth Daunton

Theses and Dissertations

Intraguild interactions affect population and community structure through a combination of competition and predation. Changes in size structure influence intraguild interactions by affecting the strength and direction of these species’ interactions. I tested for size-structured intraguild interactions in temporary pond ecosystems between marbled salamanders (Ambystoma opacum) and tiger salamanders (Ambystoma tigrinum) using an outdoor mesocosm experiment. I hypothesized that 1) A. opacum survivorship and growth would be negatively impacted when both species start at a similar size because tiger salamanders grow faster and are more aggressive 2) A. tigrinum will grow slower and have lower survivorship …

Rethinking Public Health Risk Mitigation Strategies: Recognizing The Biosocial Nature Of Covid-19, Grace Savard

CSB and SJU Distinguished Thesis

The SARS-CoV-2 virus has rippled throughout every aspect of society and influenced how we live, breathe, and interact with one another. The purpose of this thesis is to explore the interdisciplinary biosocial impacts of the SARS-CoV-2 virus and the resulting disease, COVID-19. In order to understand the complexities of caregiving during COVID-19, we conducted 75 semi-structured interviews with 55 healthcare providers across 18 states over a span of two years. This research assesses how COVID-19 risk mitigation strategies were designed with the primary intention to stop the spread of the virus rather than account for the longer-term social and biological …

Research On Characteristics, Development Trend And Funding Countermeasures Of Biology, Jie Chen, Jing Qu, Zhiyong Lou, Bing Zhu, Le Kang

Bulletin of Chinese Academy of Sciences (Chinese Version)

Strengthening the research in biology aimed at national strategic needs and major scientific frontier issues is conducive to giving full play to the strategic supporting role of biological research for national economic development and the promotion of international competitiveness. This article combs the basic characteristics and development trend of biology, summarizes the practical experience of international biological research funding, and analyzes the outstanding problems in biological research in China, such as incomplete layout, lack of sustainable and stable support, and insufficient funding for top talents and teams. By referring to the international practical experience, it puts forward policy suggestions to …

Embryological Development And Mortality Of Danio Rerio In Long Term Exposure To 17Α-Ethynylestradiol, Seong A. Jeon

University Honors Theses

Synthetic estrogens as well as other chemicals have become an increasing issue in the impact they have on marine species particularly those sensitive to the environment. A broadly used synthetic estrogen, 17α-ethynylestradiol, has become more widely known to become a growing problem to the ecology of marine wildlife due to increased usage and lack of removal in municipal sewage treatment facilities. This study observes the impact of low level EE2 in embryos following generational exposure. Zebrafish embryos, following three generations of exposure, were observed in rate of mortality, lethality, and hatch success. Concentrations as low as 1ng/L of EE2 has …

Centralized Project-Specific Metadata Platforms: Toolkit Provides New Perspectives On Open Data Management Within Multi-Institution And Multidisciplinary Research Projects, Andrew Wright Child, Jennifer Hinds, Lucas Sheneman, Sven Buerki

Biology Faculty Publications and Presentations

Open science and open data within scholarly research programs are growing both in popularity and by requirement from grant funding agencies and journal publishers. A central component of open data management, especially on collaborative, multidisciplinary, and multi-institutional science projects, is documentation of complete and accurate metadata, workflow, and source code in addition to access to raw data and data products to uphold FAIR (Findable, Accessible, Interoperable, Reusable) principles. Although best practice in data/metadata management is to use established internationally accepted metadata schemata, many of these standards are discipline-specific making it difficult to catalog multidisciplinary data and data products in a …

The Life History Of Drosophila Sperm Involves Molecular Continuity Between Male And Female Reproductive Tracts, Erin L. Mccullough, Emma Whittington, Akanksha Singh, Scott Pitnick, Mariana F. Wolfner, Steve Dorus

Biology

Interactions between sperm and the female reproductive tract (FRT) are critical to reproductive success and yet are poorly understood. Because sperm complete their functional maturation within the FRT, the life history of sperm is likely to include a molecular “hand-off” from males to females. Although such intersexual molecular continuity is likely to be widespread among all internally fertilizing species, the identity and extent of female contributions are largely unknown. We combined semiquantitative proteomics with sex-specific isotopic labeling to catalog the posttesticular life history of the sperm proteome and determine the extent of molecular continuity between male and FRTs. We show …

Determinants Of Food Selection By Bivalve Larvae, Maria Rosa, Diane K. Padilla

Biology Faculty Publications

Selection of food particles for consumption by larvae impacts nutritional gain needed for growth, development, and metamorphosis. Past work has suggested that molluscan larvae are capable of collecting food within a narrow size range. Recent studies have found evidence of size-independent food selection in molluscan larvae, but relatively little is known about the characteristics of particles that larvae preferentially capture. Therefore, we conducted experiments with the larvae of two mussels, Mytilus trossulus and Mytilus edulis, to determine whether they are selective feeders, and if so, whether we could determine the characteristics of particles selected as food. We fed larvae microalgae …

The Role Of Chemical Cues In Host Susceptibility To Fish-Parasitic Gnathiid Isopods., Clayton M. Vondriska

Student Theses and Dissertations

Parasitism is one of the most common lifestyle strategies. Ectoparasites must navigate complex environments to find their host and many rely heavily on chemical cues to do so. Gnathiid isopods are common parasites that use chemical cues to find their marine fish hosts. While gnathiids are considered host-generalists, hosts vary in their susceptibility to infestation. However, the mechanisms that mediate differential susceptibility are unknown. Here I used field and laboratory experiments to investigate if the chemo-attractiveness of hosts explains differences in susceptibility of Caribbean reef fishes to infestation by a common Caribbean gnathiid isopod, Gnathia marleyi. While G. marleyi can …

Health Risk From Toxic Metals In Wild Rice Grown In Copper Mining-Impacted Sediments, Rohan Deep Sarkar, Zhiming Zhang, Manas Warke, Rupali Datta

Michigan Tech Publications, Part 1

Northern wild rice is of great dietary and cultural importance to the Native American population in the Upper Peninsula of Michigan. Millions of tons of mine tailings were discharged into Lake Superior and other inland lakes during the copper mining boom in the early 20th century in this area. This includes L’Anse Bay, located within the Keweenaw Bay Indian Community (KBIC) reservation. Since wild rice restoration is being encouraged by the KBIC, we investigated the distribution of toxic metals in sediments, water, and wild rice and their potential impact on human health from two locations. Sand Point sloughs on L’Anse …

Genetic Variation Of The Mitochondrial Dna Control Region Across Plains Bison Herds In Usa And Canada, Gaimi Davies, Blake Mccann, Lee Jones, Stefano Liccioli, Maria Cecilia Penedo, Igor V. Ovtchinnikov

Biology Faculty Publications

Once numbering in the tens of millions, bison (Bison bison) have faced a broad range of challenges over the past century, including genetic impacts from the population bottleneck in the 1800s, and significant loss and fragmentation of habitat resulting in little opportunity for population growth on remaining small, geographically isolated reserves. To identify best practices for bison stewardship against this backdrop, managers must understand the genetic composition of existing conservation herds. This study characterized 14 plains bison (Bison bison bison) herds managed by the U.S. Department of Interior and Parks Canada Agency based on complete mtDNA …

Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven

USF Tampa Graduate Theses and Dissertations

Bolinopsis infundibulum is a wide-ranging, ubiquitous ctenophore whose fragile nature makes the collection of specimens and quantification of key predator-prey activities in controlled laboratory experiments, challenging. Thus, in situ methods often represent the best means for data collection. However, while present in surface waters, these animals can also be abundant at depths well beyond those attainable by divers. As a result, very little empirical data exist over the depth range of their natural habitats which limits our ability to assess key predator-prey interactions needed to assess their ecological role in midwater food webs. Working in Monterey Bay, California, remotely operated …

Identification Of The Global Mir-130a Targetome Reveals A Role For Tbl1xr1 In Hematopoietic Stem Cell Self-Renewal And T(8; 21) Aml, Gabriela Krivdova, Veronique Voisin, Erwin M Schoof, Sajid A Marhon, Alex Murison, Jessica L Mcleod, Martino M Gabra, Andy G X Zeng, Stefan Aigner, Brian A Yee, Alexander A Shishkin, Eric L Van Nostrand, Karin G Hermans, Aaron C Trotman-Grant, Nathan Mbong, James A Kennedy, Olga I Gan, Elvin Wagenblast, Daniel D De Carvalho, Leonardo Salmena, Mark D Minden, Gary D Bader, Gene W Yeo, John E Dick, Eric R Lechman

Faculty, Staff and Students Publications

Gene expression profiling and proteome analysis of normal and malignant hematopoietic stem cells (HSCs) point to shared core stemness properties. However, discordance between mRNA and protein signatures highlights an important role for post-transcriptional regulation by microRNAs (miRNAs) in governing this critical nexus. Here, we identify miR-130a as a regulator of HSC self-renewal and differentiation. Enforced expression of miR-130a impairs B lymphoid differentiation and expands long-term HSCs. Integration of protein mass spectrometry and chimeric AGO2 crosslinking and immunoprecipitation (CLIP) identifies TBL1XR1 as a primary miR-130a target, whose loss of function phenocopies miR-130a overexpression. Moreover, we report that miR-130a is highly expressed …

Selection On Dispersal Drives Evolution Of Metabolic Capacities For Energy Production In Female Wing-Polymorphic Sand Field Crickets, Gryllus Firmus, Lisa A. Treidel, Gessen S. Quintanilla Ramirez, Dillon J. Chung, Michael Menze, Jose P. Vazquez-Medina, Caroline M. Williams

Faculty and Staff Scholarship

Life history and metabolism covary, but the mechanisms and individual traits responsible for these linkages remain unresolved. Dispersal capability is a critical component of life histories that is constrained by metabolic capacities for energy production. Conflicting relationships between metabolism and life histories may be explained by accounting for variation in dispersal and maximal metabolic rates. We used female wing-polymorphic sand field crickets, Gryllus firmus, selected either for long wings (LW) and flight-capability or short wings (SW) and high early lifetime fecundity to test the hypothesis that selection on dispersal capability drives the evolution of metabolic capacities. While resting metabolic …

A Red Fluorescent Protein With Improved Monomericity Enables Ratiometric Voltage Imaging With Asap3, Benjamin B Kim, Haodi Wu, Yukun A Hao, Michael Pan, Mariya Chavarha, Yufeng Zhao, Michael Westberg, François St-Pierre, Joseph C Wu, Michael Z Lin

Faculty, Staff and Students Publications

A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in the presence of sample motion. We performed combinatorial multi-site mutagenesis on a cyan-excitable red fluorescent protein to create the bright and monomeric mCyRFP3, which proved to be uniquely non-perturbing when fused to the GEVI ASAP3. The green/red ratio from ASAP3-mCyRFP3 (ASAP3-R3) reported voltage while correcting for motion artifacts, allowing the visualization of membrane voltage changes in contracting cardiomyocytes and throughout the cell cycle of motile cells.

Cetacean Strandings In The Us Pacific Northwest 2000–2019 Reveal Potential Linkages To Oceanographic Variability, Amanda J. Warlick, Jessica L. Huggins, Dyanna M. Lambourn, Deborah A. Duffield, Dalin Nichole D'Alessandro, James M. Rice, John Calambokidis, M. Bradley Hanson, Joseph K. Gaydos, Multiple Additional Authors

Biology Faculty Publications and Presentations

Studying patterns in marine mammal stranding cases can provide insight into changes in population health, abundance, and distribution. Cetaceans along the United States West coast strand for a wide variety of reasons, including disease, injury, and poor nutritional status, all of which may be caused by both natural and anthropogenic factors. Examining the potential drivers of these stranding cases can reveal how populations respond to changes in their habitat, notably oceanographic variability and anthropogenic activities. In this study, we aim to synthesize recent patterns in 1,819 cetacean strandings across 26 species in the Pacific Northwest from 2000 to 2019 to …