Digital Commons Network^™

Determining The Effect Of Absorbents And Soybean Systems On Pfas Precursor Transformation, Tamia Wellington

Biological Sciences

PFAS (Per- and polyfluoroalkyl substances) refers to a class of chemicals that consist of long-chains of carbon bonded to fluorine. These are chemicals that have many industrial uses, from cosmetics to industrial fire-fighting foam. Since PFAS is so widely used and available, it has become increasingly present in water and soil, resulting in its uptake and bioaccumulation in plants. This study focused on the impacts of powdered activated carbon (PAC) and montmorillonite on the PFOS precursor N-ethyl perfluorooctane sulfonamido acetic acid (N-EtFOSAA), in soil, and in plants grown in that soil. This study focused on spiking soil with PFAS chemicals, …

Quantification Of Fibrosis And Infiltrates In The Salivary Gland, Nikhita Kumar

Biological Sciences

Fibrosis is characterized by the excessive buildup of extracellular matrix components (ECM) in tissues and organs in response to injury, chronic inflammation, auto immune disorders, and genetic alterations. Non-obese diabetic mice (NOD) are a model for Sjögren’s Syndrome. We hypothesize that NOD mice, which start to develop Sjögren’s-like disease at approximately 12 weeks of age are suffering from hyposalivation at least in part due to fibrosis of the submandibular salivary glands (SMGs). Fibrosis refers to the over-production of extracellular matrix proteins in the connective tissue compartment. To test this hypothesis 16-week-old mice were treated with either the fibrosis inhibitor Nintedanib …

Improvement Of Depressive-Like Behaviors In Hemi-Parkinsonian Rats With Non-Invasive Peripheral Focused Ultrasound Modulation, Hudy Berger

Biological Sciences

Parkinson’s disease (PD) is a chronic and progressive neurodegenerative disease with marked loss of dopaminergic neurons in the substantia nigra pars compacta (SNc). In PD, there are motor and non-motor symptoms. Depression, a non-motor symptom, is seen in 40% of the patients with PD which decreases their quality of life. For treatment-resistant depression, vagus nerve stimulation (VNS) has been shown to improve depression. Here, non-invasive focused ultrasound (FUS) is investigated as a therapeutic to improve depression-like behavior by targeting the celiac plexus since it is innervated by the vagus nerve. FUS was chosen due to its parallel mechanism to VNS. …

Trends In Nestling Body Size In A Declining Population Of American Kestrels, Kristina M. Ollo

Theses, Dissertations and Culminating Projects

American Kestrels (Falco sparverius) are small falcons that feed primarily on large insects but also small mammals and birds. During recent decades kestrel populations have been declining in North America, for reasons still unknown. One plausible explanation is that nestlings are becoming malnourished over the years from lack of food resources and are fledging at smaller sizes. Fledging at a smaller size puts kestrel nestlings at greater risk of post-fledging mortality due to starvation or predation. Here we examined long-term trends in the morphological development of American Kestrel nestlings to see if there have been any significant changes over time. …

College Of Natural Sciences Newsletter, March - May 2023, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 4, Issue 2

Page 1 Dean's Message
Page 2-7 Awards and Recognition
Page 8 March 3rd Corothers Seminar
Page 9 54th Geography Convention
Page 10 Spring 2023 Day of Scholars
Page 11 2023 URSCAD Snaps
Page 12-14 Media Coverage of CNS
Page 15 Open PRAIRIE Data

Are Rising Seas Pushing Ghost Crabs Out Of Their Comfort Zone?, Finn Gillette

Honors Theses

Ghost crabs (Ocypode quadrata) can be found on sandy beaches bordering the western Atlantic Ocean. These semiterrestrial crustaceans are often found between the swash zone and the dunes on beaches. Dunes are critical refuges for ghost crabs during storms and extreme tide events. This makes them a useful indicator species for monitoring the effects of global warming-induced sea level rise on beach biota, as their distribution patterns among beach zones may correlate with shifts found in other species. Beach surveys conducted from 2015 onward assessed the population density of ghost crabs on beaches of Horry and Georgetown counties …

Heat Stress Effects On Culex Pipiens Mosquitoes, Valeria Montoya

Theses, Dissertations and Culminating Projects

Extreme weather events such as heat waves are becoming more frequent. This is a concern for both humans and other organisms like insects. Culex pipiens f. molestus mosquitoes are primary vectors of diseases including equine encephalitis, West Nile virus, Rift valley fever, and St. Louis encephalitis. With Cx. pipiens f. molestus being the primary vector of West Nile virus in North America, it is important to understand how more frequent heat waves affect their population dynamics. In this study, I compared the thermal tolerance of larval Cx. pipiens f. molestus that were exposed to prior heat stress to those that …

Fecal Cortisol Metabolites: A Non-Invasive Method For Monitoring The Long-Term Health Of Free Ranging Brown Bears, Justin A. Pinero

All NMU Master's Theses

Ecotourism is a rapidly growing industry worldwide and has been used as a tool that can promote conservation. While ecotourism can serve as a mechanism to help conserve natural areas, increases in visitors present challenges for managers tasked with balancing conservation goals while ensuring positive visitor experiences. As such, managers and ecologists are increasingly using fecal cortisol metabolites (FCMs) to index stress associated with ecotourism. In this study, I sought to (1) quantify the relationship between blood cortisol levels and FCM concentrations in brown bears (Ursus arctos), and (2) evaluate whether ecotourism elicits a measurable stress response in …

Mechanics For Inflammatory Cytokine-Promoted Breast Cancer Progression: Osm-Induced Loxl2 Leads To Extracellular Matrix Remodeling Of Collagen I And Subsequent Metastasis, Simion C. Dinca

Boise State University Theses and Dissertations

Breast cancer has the highest prevalence of cancers in women in the USA; approximately 1 in 8 women will receive a diagnosis in their lifetime. Invasive ductal carcinoma (IDC) is the most commonly diagnosed breast cancer and presents a serious problem if it metastasizes, decreasing 5-year patient survival from ~98% to ~29%. Tumor cells grow in an environment that is known as the tumor microenvironment (TME). Due to inflammation, the TME of IDC is often saturated with neutrophil- and macrophage-derived proinflammatory cytokines including oncostatin M (OSM) and interleukin-1beta (IL-1β), both of which promote favorable conditions for metastasis. Modifications in the …

The Role Of Decorin And Biglycan In Cns Angiogenesis And Eae Recovery, Kristina Chapman

Boise State University Theses and Dissertations

Multiple Sclerosis (MS) is a severe demyelinating disease of the Central Nervous System (CNS) associated with an autoimmune response directed against myelin antigens. Angiogenesis, the formation of new blood vessels from preexisting ones, is a vital process for tissue regeneration and wound healing but is a pathological hallmark of both MS and an MS mouse model, experimental autoimmune encephalomyelitis (EAE). This type of aberrant angiogenesis is a fundamental event during an inflammatory injury, which is mutually dependent upon the intrusion of inflammatory molecules, disruption of the blood-brain barrier (BBB), and immature vessel formation. In the EAE recovery phase, this process …

Understanding Temporal Dynamics Of Plant Specialized Metabolites In Response To Simulated Browsing In Winter, Bryanna Hope Bright

Boise State University Theses and Dissertations

Plants are continually defending themselves from the herbivores that consume them, often using an array of plant specialized metabolites (PSMs). Volatile organic compounds, including monoterpenes, are one such type of PSMs that can be emitted and induced by plants in response to mechanical damage and herbivory. These volatiles serve as direct defenses against herbivores and can alert neighboring plants about potential threats, resulting in protection against future attacks. However, how these chemicals change over time in response to browsing by vertebrates in the winter has received limited attention and is crucial to interpreting how monoterpenes defend plants against vertebrate herbivores. …

Microbial Associations With Metcalf's Tryonia, Tryonia Metcalfi (Gastropoda: Cochliopidae), An Imperiled Ciénega Endemic, Russell L. Minton, Kathryn E. Perez

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The Chihuahuan Desert swamps are a hotspot for imperiled organisms including freshwater springsnails and bacteria. Many of these taxa are endemic to the desert and to the individual waterbodies where they occur. Efforts to conserve diversity in these threatened areas must account for the life history of the organisms, but also interactions between organisms including microbes. We documented the microbial assemblage associated with Tryonia metcalfi, a critically imperiled freshwater snail endemic to a ciénega system in western Texas. We identified 14 bacterial families in our snail samples and determined a core assemblage of 19 bacterial taxa (4 of which …

Effect Of Mating On Male Choosiness In Bicyclus Anynana, Ellie Hope Magera

Biological Sciences Undergraduate Honors Theses

Males mate multiply in many species, including butterflies, but little is known about whether the act of mating influences future male choosiness. We might expect mating to influence future preference, as previous studies have shown that pre-mating social experiences affect future male mate choice. To determine if initial male mate choice affects subsequent male mating decisions, we conducted behavioral assays using the subtropical nymphalid butterfly Bicyclus anynana. We tested three alternative hypotheses: 1) initial mating is a positive experience that strengthens male preference for the previously encountered phenotype; 2) males prefer mating with a different phenotype in their second …

Understanding The Role Of Heterodisulfide Reductase During Nitrogen Fixation By Methanosarcina Acetivorans, Grant Shelnut

Biological Sciences Undergraduate Honors Theses

Methanosarcina acetivorans is a methanogenic archaeon (methanogen) that is important for several processes in our biosphere. A feature of interest within this methanogen is the enzyme nitrogenase which catalyzes the reduction of unusable dinitrogen gas (N₂) into a usable form of nitrogen, ammonia (NH₃). How electrons are transferred within M. acetivorans to nitrogenase during N₂ reduction is unknown. Heterodisulfide reductase (Hdr) is a key enzyme known to reduce the electron carrier protein ferredoxin, which is a direct electron donor to nitrogenase in bacteria. I hypothesized that cytoplasmic HdrA2B2C2 functions in electron transfer to nitrogenase and …

Hormonal Control Of Osmoregulatory Proteins In The Intestine Of Atlantic Killifish (Fundulus Heteroclitus), Karma Hine

Biological Sciences Undergraduate Honors Theses

The Atlantic killifish (Fundulus heteroclitus) is a euryhaline fish, and thus able to maintain ionic and osmotic homeostasis in a wide range of salinities. When exposed to the dehydrating marine environment, the lost water is replenished through drinking seawater (SW) which is processed and absorbed in the intestine through the involvement of various cellular effectors. Intestinal water transport is osmotically driven by the active removal of NaCl from the gut lumen to the blood coupled to the precipitation of divalent Mg²⁺ and Ca²⁺ due to the secretion of bicarbonate. These processes allow for successive removal of …

Determining The Functional Roles Of Short Open Reading Frames In Mycobacterium Smegmatis, Lauren Fox

Biological Sciences

The study examines the roles of a subset of short open reading frames (sORFs) in M. smegmatis using targeted mutagenesis and the subsequent examination of phenotypes associated with sORF mutation or overexpression. sORFs are defined as stretches of nucleic acid encoding a protein with a maximum of 50 amino acids. Genome annotation pipelines overlook sORFs encoding small proteins (sproteins) and, thus, sproteins in bacteria have gone unnoticed, even those that are expressed at levels similar to larger, well-described proteins. As a result of recent advances in ribosome profiling and mass spectrometry, hundreds of previously unannotated sORFs have been identified, increasing …

Population Genetics Among Rhipicephalus Sanguineus Ticks In The Lower Rio Grande Valley, Bianca Liana Guerra

Theses and Dissertations

Rhipicephalus sanguineus is a common tick species in the lower Rio Grande Valley of both human and veterinary concern. Two lineages of R. sanguineus have been described across the United States known as the tropical and temperate lineage. Both lineages can be differentiated morphologically, genetically, biologically, and by vector competence. The species name for both lineages has not been well established therefore this thesis will utilize their widely used identification. Both lineages have been identified in Texas. While the distribution of these lineages has been well-defined in some regions across the world, recent studies have observed sympatric populations in certain …

Road Mitigation Structure And Road Effect Zone Impacts On Rodent Community Composition, Activity, And Predator-Prey Dynamics In South Texas, Adam F. Sanjar

Theses and Dissertations

The effects of road mortality mitigation structures on rodent activity and rodent community composition are not fully understood, both on the regional scale of south Texas and in the broader field of road ecology. This is primarily due to the low conservation priority of most rodent species and the difficulty of noninvasively surveying for rodents effectively. This thesis focused on characterizing how rodents were impacted by both roads and mitigation structures in south Texas, and if rodents are a significant factor to consider when studying the road ecology of rodent predators in south Texas, particularly ocelots, bobcats (Lynx rufus …

Vibroacoustic Response Of The Tympanic Membrane To Hyoid-Borne Sound Generated During Echolocation In Bats, Chelsie Snipes

Electronic Theses and Dissertations

The hyoid apparatus in laryngeally echolocating bats forms a mechanical connection between the larynx and auditory bullae and has been hypothesized to transfer the outgoing echolocation call to the middle ear during echolocation call emission. We used µCT data to build models of the hyoid apparatus and middle ear from six species of bats and used finite element modeling (FEM) to measure the vibroacoustic response of the tympanic membrane due to hyoid-borne sound generated during echolocation. We found that hyoid-borne sound in all six species stimulated the eardrum within a range likely heard by bats. Although there were minor differences …

Identifying Direct Foxo Targets Through Chromatin Immunoprecipitation Assays With Sequencing, Alma Lopez

Theses and Dissertations

Glioblastoma Multiforme (GBM) and basal-like breast cancer (BBC) are aggressive cancers associated with poor prognoses. Basal-like breast cancer (BBC) and glioblastoma multiforme (GBM) are poor prognosis cancers that lack effective targeted therapies. A deregulation of FOXOs has been linked to cancer, as they regulate the expression of genes involved in multiple cellular processes. Canonically, the FOXO family of proteins is generally considered tumor suppressors, but evidence suggests that they may be involved in cancer metabolism as well as angiogenesis. Research in our lab has found that these factors play a role in glycolysis and the developmental WNT pathway. Here we …

Urbanization Affects Web Abundance And Aggregation Of A Funnel‑Weaving Spider, Agelenopsis Pennsylvanica (Agelenidae), Brandi J. Pessman, Madison Hays, Earl Agpawa, Eileen Hebets

Eileen Hebets Publications

Animals distribute themselves within habitats based on a variety of environmental conditions, including those impacted by urbanization. Suspected global declines in urban arthropod biodiversity have required that we examine how urban conditions affect the distribution of this ecologically important group. Throughout North America, funnel-weaving spiders (Agelenidae) are prevalent across urban habitats and actively choose sites to build webs. We compared Agelenopsis pennsylvanica abundance and distribution between two distinct urban habitats: an urban center (university campus) and an urban forest (city park). These urban habitats differed significantly in features like plant diversity and proximity to roads and highways. We searched along …

Toward Accelerometer Recording Of Pit Viper Foraging Behavior In Nature: Validation And Case Study With Timber Rattlesnakes (Crotalus Horridus), Morgan L. Thompson

Biology Theses

Accelerometer dataloggers are becoming increasingly common in field studies of animal behavior. Among the most difficult study subjects, and consequently, the most ideal candidates for accelerometer applications, are those for which simultaneous observation of key fitness-determining behaviors, such as foraging, across a sample of individuals in the wild is traditionally impossible or restricted to the use of proxy measures. This is the case for many solitary vertebrate predators, such as all pit vipers (Viperidae; Crotalinae). Large-bodied pit vipers are ambush (sit-and-wait) specialists that represent uniquely challenging and intriguing study subjects in predator ecology. Unlike many comparable avian or mammalian terrestrial …

Uncovering Molecular Targets To Overcome Immunosuppression In Non-Small Cell Lung Cancer With Acquired Tki Resistance, Sonia A. Patel

Dissertations and Theses (Open Access)

Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related deaths worldwide. Targeted therapeutic agents, such as epidermal-like growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) or monoclonal antibodies targeting vascular endothelial growth factor (VEGF/R), can effectively inhibit upregulated signaling pathways driving tumorigenesis in NSCLC and many other cancers. Unfortunately, however, resistance to such targeted therapies inevitably arise in most patients and can occur through a variety of resistance mechanisms including genomic alterations and upregulation of bypass pathways. Additionally, patients who have acquired resistance to these targeted agents typically have tumors characterized by an immunosuppressive tumor microenvironment and thus …

Determination Of Cadmium Uptake In Crassostrea Virginica Shell Under Controlled Conditions, Joseph John Pavelites Ii

Graduate Theses and Dissertations (2019 - present)

The objective of this thesis was to meet growing demand for the development of environmental biomonitors that protect ecosystems and public health. To do this, I determined the potential of oyster shell as a bioindicator of cadmium (Cd) in the environment by determining the mode of Cd uptake and relationships between Cd concentrations in the environment, shell, and soft tissues of juvenile eastern oysters (Crassostrea virginica Gmelin). I performed a review of the literature on the ability of oyster shell to retain metal contaminants and the factors that could affect this process (Chapter 2). I then reared C. virginica …

Cyclic-Di-Gmp Regulates Salmonella Typhimurium Infection Of Epithelial Cells And Macrophages, Abdulafiz Musa

Electronic Theses and Dissertations

Regulation of the bacterial second messenger cyclic-di-GMP in Salmonella Typhimurium allows it to delicately alter phenotypes to optimize invasion and survive intracellularly in epithelial cells and macrophages to become virulent and cause infection. Cyclic-di-GMP concentration is regulated by the presence of external stimuli, sensory diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), and cyclic-di-GMP binding effectors. Previous studies established that maintenance of low cyclic-di-GMP concentrations is required for survival in macrophages, and that deletion of 3 active PDEs reduces this survival. Here I showed that these 3 PDEs also influenced the infection of epithelial cells. Further studies re-established the decreased survival in …

Characterization Of Neutrophil Extracellular Traps In Naked Mole-Rats: A Step Towards Cancer Resistance, Thomas Abraham Smith

Undergraduate Honors Capstone Projects

Cancer is the second leading cause of death in the United States, exceeded only by heart disease. One of every five deaths in the United States is due to cancer. A growing area of research involves the analysis of cancer resistant traits in other species to understand their biological mechanisms and eventually make translations to human cancer research and clinical treatment. Because of their remarkable cancer resistance, the naked mole-rat (NMR) is a prime subject for this research, and various studies have already suggested that the immune mechanisms of the NMR might be harnessed for human cancer therapies^1-4,7. …

Protein Nutrition And Immunity In Male Bumblebees (Bombus Impatiens), Carson Stoker

Undergraduate Honors Capstone Projects

Male bumble bees play a vital but understudied role in their colonies. They are essential to colony reproduction and success, despite not emerging until the end of the colony's life cycle. Even so, the biology of male bumble bees is not well understood, which leaves our understanding of colony health incomplete. This knowledge gap is important because bumblebee populations are declining, which threatens the ecosystems and industries which rely on them for pollination. This study aimed to understand how two understudied factors of bumble bee health—diet and immunity—are related in males. Pollen, a bee’s main source of protein, has been …

Carbon Quantum Dots' Effects On The Structure And Function Of Neurodegenerative And Non-Neurodegenerative Associated Proteins, Shima Masoudi Asil

Open Access Theses & Dissertations

Protein folding is an important physical process in which the sequences of polypeptides fold into a three-dimensional biological active structure. Misfolding is the conversion of a protein molecule into a structure unlike the native form, and therefore misfolded proteins have a strong tendency to aggregate and make fibrils. Amyloid aggregation is constituted by misfolded amyloidogenic proteins. Neurodegenerative diseases, such as Alzheimer’s Disease (AD), stem from the abnormal accumulation of harmful proteins, including Amyloid-ß peptides, tau protein, and the presynaptic protein α-synuclein in the nervous system. Alzheimer's disease is the most common neurodegenerative disorder that causes memory and learning deficits and …

We Travel Together: Examining The Drivers And Functions Of Animal Movement In Biotic Seed Dispersal, Binod Borah

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Plants and frugivorous animals exist in mutually beneficial relations, as these animals feed on fruits, ingest the seeds, and carry them away from the parent trees. Such dispersion of seeds over space helps them colonize new habitats, escape high mortality rates near their parent trees, and avoid competition with conspecifics. Therefore, seed dispersing animal movement can be critical for the persistence of plant populations. Yet what drives such seed disperser movement is often less understood and how it affects seed dispersal is little explored. In my dissertation, I investigate multiple drivers of seed disperser movement, link movement to potential seed …

Thermal Tolerance, Physiology, And Microhabitat Use Of Eleutherodactylus Coqui Across An Elevational Gradient In Hawai‘I, Jack Marchetti

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The coqui frog (Eleutherodactylus coqui) is an invasive species in Hawai‘i, and has spread across much of the island. While elevational temperature differences are thought to restrict the coqui’s spread, it is unclear if they have resulted in changes to the coqui’s physiology and microhabitat use as it has expanded into higher elevations.

We first sought to determine if the coqui’s substrate use and baseline physiology differs between elevations. We found that frogs at high elevation were found closer to the forest floor and used different substrates than frogs from low and mid-elevations. This change in microhabitat use …