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Diversity Of The Unionidae In The Rocky River, Ohio, Robert A. Krebs, Louie J. Rundo Jan 2005

Diversity Of The Unionidae In The Rocky River, Ohio, Robert A. Krebs, Louie J. Rundo

Biological, Geological, and Environmental Faculty Publications

We surveyed the freshwater mussels (Mollusca: Bivalvia: Unionidae) of the Rocky River, Ohio, USA, a river surrounded by suburban development. The survey produced 253 live unionid specimens and 572 empty shells. Most occurred in the west branch. Species living in the West Branch Rocky River included Anodontoides ferussacianus, Elliptio dilatata, Lampsilis cardium, Lampsilis radiata luteola, Lasmigona compressa, Lasmigona costata, Pyganodon grandis grandis, Strophitus undulatus undulatus, and Toxolasma parvus. Two additional species (Utterbackia imbecillis and Villosa iris iris) were represented as dead shells. Three species (Potamilis alatus, Quadrula quadrula and Leptodea fragilis) were found only near the …


Density And Cover Preferences Of Black-And-Rufous Elephant-Shrews (Rhynchocyon Petersi) In Chome Forest Reserve, Tanzania, Stephanie Coster, David O. Ribble Jan 2005

Density And Cover Preferences Of Black-And-Rufous Elephant-Shrews (Rhynchocyon Petersi) In Chome Forest Reserve, Tanzania, Stephanie Coster, David O. Ribble

Biology Faculty Research

The objective of this study was to determine the density and habitat preference of the Black-andrufous elephant-shrew (Rhynchocyon petersi) in Chome Forest Reserve, Tanzania. Chome Forest (143km2) is located in the South Pare Mountains and provides critical habitat for endangered R. petersi. Twelve 300m transects were cut through the centre of the forest in an east-west direction and the number of elephant-shrew nests within 2.5 meters on each side of the transects was recorded. The mean number of nests per 100m transect (0.39 ± 0.47 [1SE]) translated to a density estimate of 19 elephant-shrews per …


Assessing Cultural And Ecological Variation In Ethnobiological Research: The Importance Of Gender, Jeanine M. Pfeiffer Jan 2005

Assessing Cultural And Ecological Variation In Ethnobiological Research: The Importance Of Gender, Jeanine M. Pfeiffer

Faculty Publications, Environmental Studies

Contending that a significant portion of current ethnobiological research continues to overlook cultural variation in traditional ecological knowledge (TEK) and practice, this paper explores the potential impacts of gender-imbalanced research on data collection, hypothesis testing, and the formulation and application of ethnobiological inventories and theories. A multidisciplinary review of over 220 studies addresses commonly held stereotypes underlying gender-imbalanced field research and demonstrates the need for more inclusive, finely-tuned studies which disaggregate indigenous knowledge and practice by gender. The paper outlines factors underlying gender-based spatial and temporal variation in ecosystem exposure and traditional ecological knowledge in rural societies worldwide, and discusses …


The 19th Century Excavation Of Kent's Cavern, England, Donald A. Mcfarlane, Joyce Lundberg Jan 2005

The 19th Century Excavation Of Kent's Cavern, England, Donald A. Mcfarlane, Joyce Lundberg

WM Keck Science Faculty Papers

Between 1858 and 1880, William Pengelly developed revolutionary new techniques for the archeological and paleontological excavation of cave deposits. His work at Brixham Cave and Kent’s Cavern, England, yielded tens of thousands of specimens from the mid-Pleistocene to the Holocene, settled the intellectual debate over the co-existence of humans and extinct mammals, and accumulated an unparalleled resource for continued study. Although the Brixham Cave work was thoroughly summarized in print, Pengelly never published the plans of his much more thorough and extensive excavations at Kent’s Cavern. Here we present a reconstructed plan of the Pengelly excavations that we hope will …


The Effects Of Ecotourism On Polar Bear Behavior, Gillian Eckhardt Jan 2005

The Effects Of Ecotourism On Polar Bear Behavior, Gillian Eckhardt

Electronic Theses and Dissertations

Polar bears spend the majority of their lives on the sea ice, where they gain access to seals and mates. In western Hudson Bay, the sea ice melts for three to four months in the summer, and polar bears there are forced onto land. These bears live on their fat reserves for the duration of the iceless period, until temperatures get colder in the fall and freeze up begins. The aggregation of polar bears near Churchill, Manitoba during the ice free period has led to a thriving tourist industry, with a large influx of tourists visiting Churchill in the fall …


The Social Environment Affects Mate Guarding Behavior In Japanese Beetles, Popillia Japonica, Yoriko Saeki, Kipp C. Kruse, Paul Switzer Jan 2005

The Social Environment Affects Mate Guarding Behavior In Japanese Beetles, Popillia Japonica, Yoriko Saeki, Kipp C. Kruse, Paul Switzer

Faculty Research & Creative Activity

The effect of the social environment on post-copulatory mate guarding duration in Japanese beetles, (Popillia japonica Newman), was examined in a laboratory experiment. The mate guarding durations of beetles in different sex ratios and densities were observed for 10 hours. Guarding duration was longer when females were larger, suggesting the presence of ‘cryptic’ male mate choice. Densities, but not sex ratio, affected the duration of guarding bouts, with males guarding for longer at higher densities. This result implies that males increase their guarding duration under conditions in which their female may be likely to be encountered by other males. The …


A Theoretical Approach To Assessing Annual Energy Balance In Gray Whales (Eschrichtius Robustus), Nathalie Lucie Elizabeth Greenwald Jan 2005

A Theoretical Approach To Assessing Annual Energy Balance In Gray Whales (Eschrichtius Robustus), Nathalie Lucie Elizabeth Greenwald

Electronic Theses and Dissertations

While direct measurements of energetic demands are nearly impossible to collect on large cetaceans, comprehensive bioenergetic models can give insights on such parameters by combining physiological and ecological knowledge. This model was developed to estimate necessary food intake of gray whales, Eschrichtius robustus, of the Eastern North Pacific stock. Field Metabolic Rates (FMR) for gray whales were first estimated based on various assumptions (e.g. volumetric representation of gray whales, extent of their feeding season, and blubber depth distribution) using morphometric data, energetic costs, and food assimilation according to age and gender specific requirements. Food intake rates for gray whales of …


Historical Responses Of Marine Turtles To Global Climate Change And Juvenile Loggerhead Recruitment In Florida, Joshua Reece Jan 2005

Historical Responses Of Marine Turtles To Global Climate Change And Juvenile Loggerhead Recruitment In Florida, Joshua Reece

Electronic Theses and Dissertations

Marine turtle conservation is most successful when it is based on sound data incorporating life history, historical population stability, and gene flow among populations. This research attempts to provide that information through two studies. In chapter I, I identify historical patterns of gene flow, population sizes, and contraction/expansion during major climatic shifts. In chapter II, I reveal a life history characteristic of loggerhead turtles previously undocumented. I identify a pattern of juvenile recruitment to foraging grounds proximal to their natal nesting beach. This pattern results in a predictable recruitment pattern from juvenile foraging ground aggregations to local rookeries. This research …


Native And Invasive Competitors Of The Eastern Oyster Crassostrea Virginica In Mosquito Lagoon, Florida, Michelle Boudreaux Jan 2005

Native And Invasive Competitors Of The Eastern Oyster Crassostrea Virginica In Mosquito Lagoon, Florida, Michelle Boudreaux

Electronic Theses and Dissertations

Populations of Crassostrea virginica within Mosquito Lagoon, Florida have recently undergone significant die-offs, which are a subject of major concern. Restoration efforts within Mosquito Lagoon are focusing on reconstructing the three-dimensional reef habitats. Before effective protocols can be established, however, important questions about the sources of juvenile and adult oyster mortality must be answered. Potential causes of Crassostrea virginica mortality in the Indian River Lagoon system include sediment loads, competition, predation, and disease. My research focused on the interactions between oysters and the competitors that may affect the settlement, growth, and survival of Crassostrea virginica. The four objectives of my …


Habitat And Exposure Modelling For Ecological Risk Assessment: A, T. Edwin Chow, Karen F. Gaines, Michael E. Hodgson, Machelle D. Wilson Jan 2005

Habitat And Exposure Modelling For Ecological Risk Assessment: A, T. Edwin Chow, Karen F. Gaines, Michael E. Hodgson, Machelle D. Wilson

Faculty Research & Creative Activity

Contamination has a dramatic impact on the health of ecosystem and habitat suitability for the inhabited flora and fauna. The Environmental Protection Agency (EPA) mandates an ecological risk assessment (ERA) that evaluates the potential adverse impact of any anthropogenic activities on the ecosystem (US Environmental Protection Agency, 1997. Ecological Risk Assessment Guidance for Superfund: Process for Designing and Conducting Ecological Risk Assessment. EPA/630/R-021011, Washington, DC). This study provides a general framework and specific procedures to predict the contaminant exposure of midsized mammals using a geographical information system (GIS)-based Monte Carlo simulation model. The model was applied to the raccoons (Procyon …


Allosteric Models For Multimeric Proteins: Oxygen-Linked Effector Binding In Hemocyanin, Michael A. Menze, Nadja Hellman, Heinz Decker, Manfred K. Grieshaber Jan 2005

Allosteric Models For Multimeric Proteins: Oxygen-Linked Effector Binding In Hemocyanin, Michael A. Menze, Nadja Hellman, Heinz Decker, Manfred K. Grieshaber

Faculty Research & Creative Activity

In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobster Homarus Vulgaris during hypoxia, increase hemocyanin oxygen affinity and decrease cooperativity of oxygen binding. To derive a model that describes the mechanism of allosteric interaction between hemocyanin and oxygen in the presence of urate or caffeine, studies of oxygen, urate, and caffeine binding to hemocyanin were performed. Exposure of lobster hemocyanin to various pH values between 7.25 …


The Relationship Between Community Diversity And Exotic Plants: Cause Or Consequence Of Invasion?, Scott J. Meiners, Mary L. Cadenasso Jan 2005

The Relationship Between Community Diversity And Exotic Plants: Cause Or Consequence Of Invasion?, Scott J. Meiners, Mary L. Cadenasso

Faculty Research & Creative Activity

Invasion ecology has suffered from the artificial separation of invasibility and impact processes in understanding the ationship between diversity and plant invasion. By studying these independently functioning stages of invasion in concert, we can gain great insight into the biological causes and consequences of invasions, and develop crucial information for the generation of adequate management strategies. Our conceptual framework provides a structure to synthesize the current body of research, suggests research needed to fill the gaps in understanding and to organize results from future research. The framework is a powerful tool to guide ecological understanding of the relationship between invasion …


Morphological Divergence Of Native And Recently Established Populations Of White Sands Pupfish (Cyprinodon Tularosa), Michael L. Collyer, James M. Novak, Craig A. Stockwell Jan 2005

Morphological Divergence Of Native And Recently Established Populations Of White Sands Pupfish (Cyprinodon Tularosa), Michael L. Collyer, James M. Novak, Craig A. Stockwell

Faculty Research & Creative Activity

We used landmark-based geometric morphometric methods to describe patterns of body shape variation and shape covariation with size among populations of the threatened White Sands Pupfish (Cyprinodon tularosa), a species that occurs in dissimilar aquatic habitats. White Sands Pupfish populations include two genetically distinct, native populations that have been historically isolated in Salt Creek, a saline river, and Malpais Spring, a brackish spring. In addition, two populations were established approximately 30 years before this study by translocation of fish from Salt Creek to Lost River (a saline river) and Mound Spring (a brackish spring). We found significant body shape variation …


Social Organization Of The Eastern Rock Elephant-Shrew (Elephantulus Myurus): The Evidence For Mate Guarding, David O. Ribble, M. R. Perrin Jan 2005

Social Organization Of The Eastern Rock Elephant-Shrew (Elephantulus Myurus): The Evidence For Mate Guarding, David O. Ribble, M. R. Perrin

Biology Faculty Research

Understanding the costs and benefits of defending solitary females, or mate guarding, may be the key to understanding the evolution of monogamy in most mammals. Elephant-shrews, or sengis, are a unique clade of small mammals that are particularly attractive for studies of mate guarding. We studied the spatial organization of Eastern Rock Sengis (Elephantulus myurus) in KwaZulu-Natal, South Africa, from August – December 2000. Our objectives were to describe the home ranges of males and females using radiotelemetry, noting the sizes and overlap of adjacent ranges and how the spatial organization changes through time. Males and females were …


Gambelia Wislizenii (Long-Nosed Leopard Lizard). Arboreal Behavior., Stephen C. Adolph, Sean P. Fogarty '06 Jan 2005

Gambelia Wislizenii (Long-Nosed Leopard Lizard). Arboreal Behavior., Stephen C. Adolph, Sean P. Fogarty '06

All HMC Faculty Publications and Research

In the North American southwest, leopard lizards (genus Gambelia) are characteristically ground dwelling (Stebbins 2003. A Field Guide to Western Reptiles and Amphibians, 3rd ed., Houghton-Mifflin, New York. 533 pp.; Smith 1946. Handbook of Lizards, Comstock Publishing Co., Ithaca, New York. 557 pp.). In G. wislizenii, climbing is not mentioned in several substantive ecological studies of this species (Parker and Pianka 1976. Herpetologica 32:95-114; Tanner and Krogh 1974. Herpetologica 30:63-72), and to our knowledge, only a few seconds exist of climbing in this species. Clark (1974. Trans. Kansas Acad. Sci. 77:68) observed a G. wislizenii perched 30 cm …


Relationship Between Habitat Distribution, Growth Rate, And Plasticity In Congeneric Larval Dragonflies, S. J. Mccauley Jan 2005

Relationship Between Habitat Distribution, Growth Rate, And Plasticity In Congeneric Larval Dragonflies, S. J. Mccauley

Biological Sciences

The relationship between habitat distribution, growth rate, and plasticity was examined in the larvae of three species of dragonfly in the genus Libellula L., 1758. Growth rates were compared under three conditions: in the absence of predation risk, in the presence of sunfish (Lepomis macrochirus Rafinesque, 1819; Pisciformes: Centrachidae), and in the presence of invertebrate predators. I assessed how the habitat distributions of the three species of dragonfly, specifically how commonly they occur with fish, were related to growth rates and to the level of growth plasticity under different levels of perceived predation risk. There was a negative relationship …


The Bell Family Legacies, William D. Stansfield Jan 2005

The Bell Family Legacies, William D. Stansfield

Biological Sciences

No abstract provided.


A Multiple-Objective Decision Analysis Of Stakeholder Values To Identify Watershed Improvement Needs, Jason R. W. Merrick, Gregory S. Parnell, Jamison Barnett, Margot Garcia Jan 2005

A Multiple-Objective Decision Analysis Of Stakeholder Values To Identify Watershed Improvement Needs, Jason R. W. Merrick, Gregory S. Parnell, Jamison Barnett, Margot Garcia

Statistical Sciences and Operations Research Publications

The paper describes the use of multiple objective decision analysis to qualitatively and quantitatively assess the quality of an endangered watershed and guide future efforts to improve the quality of the watershed. The Upham Brook watershed is an urban watershed that lies at the interface of declining inner city Richmond, Virginia and growth-oriented Henrico County. A section of stream within the watershed has been identified as so dangerously polluted that it threatens the health of the residents who live within the watershed boundaries. With funding provided by the National Science Foundation, the Upham Brook watershed project committee was formed to …


Decline In Avoidance Of Predator Chemical Cues: Habituation Or Biorhythm Shift?, Aaron M. Sullivan, John C. Maerz, Dale M. Madison Jan 2005

Decline In Avoidance Of Predator Chemical Cues: Habituation Or Biorhythm Shift?, Aaron M. Sullivan, John C. Maerz, Dale M. Madison

Faculty Publications - Department of Biological & Molecular Science

No abstract provided.


Abejas Altoandinas De Colombia: Guía De Campo, Victor H. Gonzalez, Monica Ospina, Daniel J. Bennett Jan 2005

Abejas Altoandinas De Colombia: Guía De Campo, Victor H. Gonzalez, Monica Ospina, Daniel J. Bennett

Faculty Publications

This guide is a contribution to the knowledge and conservation of bees, a group of very interesting and unknown to Colombia insects. Alrededor de 70 especies, agrupadas en 25 géneros habitan los Andes colombianos por encima de los 2.500 msnm. About 70 species, grouped in 25 genera inhabit the Colombian Andes above 2,500 meters. De éstas, por lo menos 15, son especies nuevas para la ciencia o están en proceso de descripción. Of these, at least 15 are new to science or are in the process of describing species. Igualmente, la guía busca incrementar la conciencia sobre la importancia de …


Evaluation Of A New Method For Measuring Salamanders, Leroy J. Walston, Stephen J. Mullin Jan 2005

Evaluation Of A New Method For Measuring Salamanders, Leroy J. Walston, Stephen J. Mullin

Faculty Publications

Collection of morphometric data is essential to many field studies of amphibian populations. One of the most common measurements obtained from salamanders is snout-to-vent length (SVL), a parameter useful in studies of sexual dimorphism (Bovero et al. 2003), demography (Semlitsch 1985), and systematics (Carlin 1997). Measuring salamander lengths in the field is challenging because of their small size, slimy texture, and propensity to maintain a nonlinear body position. Time expenditure is another concern, especially when researchers process large numbers of salamanders. Different measurement techniques vary in accuracy and precision, limiting the reliability of the data so that comparisons cannot be …


Allometry, Sexual Size Dimorphism, And Niche Partitioning In The Mediterranean Gecko (Hemidactylus Turcicus), James B. Johnson, Lance D. Mcbrayer, Daniel Saenz Jan 2005

Allometry, Sexual Size Dimorphism, And Niche Partitioning In The Mediterranean Gecko (Hemidactylus Turcicus), James B. Johnson, Lance D. Mcbrayer, Daniel Saenz

Faculty Publications

Hemidactylus tucrius is a small gekkonid lizard native to the Middle East and Asia that is known to exhibit sexual dimorphism in head size. Several potential explanations exist for the evolution and maintenance of sexual dimorphism in lizards. We tested 2 of these competing hypotheses concerning diet partitioning and differential growth. We found no differences in Average meal size (volume) or in any single dimension of prey size for similarly sized males and females. allometric patterns of increases in head size also were measured in males and females. We found that males exhibited a mixture of isometric and positively allometric …


A Spatially Explicit Model Of The Wild Hog For Ecological Risk Assessment Activities At The Department Of Energy's Savannah River Site, Karen F. Gaines, Dwayne E. Porter, Tracy Punshon, I. Lehr Brisbin Jr. Jan 2005

A Spatially Explicit Model Of The Wild Hog For Ecological Risk Assessment Activities At The Department Of Energy's Savannah River Site, Karen F. Gaines, Dwayne E. Porter, Tracy Punshon, I. Lehr Brisbin Jr.

Faculty Research & Creative Activity

In North America, wild hogs (Sus scrofa) are both sought after as prime game and despised due to their detrimental impacts to the environment from their digging and rooting behavior. They are also a potentially useful indicator species for environmental health for both ecological- and human-based risk assessments. An inductive approach was used to develop probabilistic resource selection models using logistic regression to quantify the likelihood of hogs being in any area of the Department of Energy’s 805 km2 Savannah River Site (SRS) in west-central South Carolina. These models were derived by using available SRS hog hunt data from 1993–2000 …


The Development Of A Spatially Explicit Model To Estimate, Karen F. Gaines, C. Shane Boring, Dwayne E. Porter Jan 2005

The Development Of A Spatially Explicit Model To Estimate, Karen F. Gaines, C. Shane Boring, Dwayne E. Porter

Faculty Research & Creative Activity

A spatially explicit model of raccoon (Procyon lotor) distribution for the U.S. Department of Energy’s (DOE) Savannah River Site (SRS) in west-central South Carolina was developed using data from a raccoon radio-telemetry study and visualized within a Geographic Information System (GIS). An inductive approach was employed to develop three sub-models using the ecological requirements of raccoons studied in the following habitats: (1) man-made reservoirs, (2) bottomland hardwood/ riverine systems, and (3) isolated wetland systems. Logistic regression was used to derive probabilistic resource selection functions using habitat compositional data and landscape metrics. The final distribution model provides a spatially explicit probability …


Rodents As Receptor Species At A Tritium, Angel Kelsey-Wall, John C. Seaman, Charles H. Jegoe, Cham E. Dallas, Karen F. Gaines Jan 2005

Rodents As Receptor Species At A Tritium, Angel Kelsey-Wall, John C. Seaman, Charles H. Jegoe, Cham E. Dallas, Karen F. Gaines

Faculty Research & Creative Activity

New methods are being employed on the Department of Energy’s Savannah River Site to deal with the disposal of tritium, including the irrigation of a hardwood/pine forest with tritiated water from an intercepted contaminant plume to reduce concentrations of tritium outcropping into Fourmile Branch, a tributary of the Savannah River. The use of this system has proven to be an effective means of tritium disposal. To evaluate the impact of this activity on terrestrial biota, rodent species were captured on the tritium disposal site and a control site during two trapping seasons in order to assess tritium exposure resulting from …


The Effects Of Resource Availability On Alternative Mating Tactics In Guppies (Poecilia Reticulata), Gita R. Kolluru, Gregory F. Grether Jan 2005

The Effects Of Resource Availability On Alternative Mating Tactics In Guppies (Poecilia Reticulata), Gita R. Kolluru, Gregory F. Grether

Biological Sciences

Food availability can influence the optimal allocation of time and energy among alternative behaviors such as foraging, courting, and competing for mates. If populations differ consistently in food availability, selection may cause geographic divergence in allocation strategies. At the opposite extreme, a norm of reaction may evolve such that food intake influences the allocation strategy of individuals in the same way in all populations. Between these two extremes, food intake reaction norms may diverge genetically among populations. For example, at sites where food is scarce, selection may strengthen the effect of food intake on behavior, whereas at sites with abundant …


The Large Genome Constraint Hypothesis: Evolution, Ecology And Phenotype, Charles A. Knight, Nicole A. Molinari, Dmitri A. Petrov Jan 2005

The Large Genome Constraint Hypothesis: Evolution, Ecology And Phenotype, Charles A. Knight, Nicole A. Molinari, Dmitri A. Petrov

Biological Sciences

• Background and Aims If large genomes are truly saturated with unnecessary ‘junk’ DNA, it would seem natural that there would be costs associated with accumulation and replication of this excess DNA. Here we examine the available evidence to support this hypothesis, which we term the ‘large genome constraint’. We examine the large genome constraint at three scales: evolution, ecology, and the plant phenotype.

• Scope In evolution, we tested the hypothesis that plant lineages with large genomes are diversifying more slowly. We found that genera with large genomes are less likely to be highly specious – suggesting a large …


Does Intracellular Metabolite Diffusion Limit Post-Contractile Recovery In Burst Locomotor Muscle?, Stephen T. Kinsey, Pragyansri Pathi, Kristin M. Hardy, Amanda Jordan, Bruce R. Locke Jan 2005

Does Intracellular Metabolite Diffusion Limit Post-Contractile Recovery In Burst Locomotor Muscle?, Stephen T. Kinsey, Pragyansri Pathi, Kristin M. Hardy, Amanda Jordan, Bruce R. Locke

Biological Sciences

Post-metamorphic growth in the blue crab entails an increase in body mass that spans several orders of magnitude. The muscles that power burst swimming in these animals grow hypertrophically, such that small crabs have fiber diameters that are typical of most cells (600μm). Thus, as the animals grow, their muscle fibers cross and greatly exceed the surface area to volume ratio (SA:V) and intracellular diffusion distance threshold that is adhered to by most cells. Large fiber size should not impact burst contractile function, but post-contractile recovery may be limited by low SA:V and excessive intracellular diffusion distances. A number of …


The Effect Of Changing Environmental Stimuli On Betta Splendens Behavior, Jessica Kingsley Jan 2005

The Effect Of Changing Environmental Stimuli On Betta Splendens Behavior, Jessica Kingsley

Honors Capstones

Capstone submitted as a graduation requirement for the BSU Honors program.


Yeast Puf3 Mutants Reveal The Complexity Of Puf-Rna Binding And Identify A Loop Required For Regulation Of Mrna Decay, Wendy Olivas, S. Houshmandi Jan 2005

Yeast Puf3 Mutants Reveal The Complexity Of Puf-Rna Binding And Identify A Loop Required For Regulation Of Mrna Decay, Wendy Olivas, S. Houshmandi

Biology Department Faculty Works

The eukaryotic Puf proteins regulate mRNA translation and degradation by binding the 3' untranslated regions of target mRNAs. Crystal structure analysis of a human Puf bound to RNA suggested a modular mode of binding, with specific amino acids within each of eight repeat domains contacting a single nucleotide of the target RNA. Here we study the mechanism by which the yeast Puf3p binds and stimulates the degradation of COX17 mRNA. Mutation of the predicted RNA-binding positions of Puf3p to those found in Puf5p demonstrated that a single amino acid change in Puf3p abolished detectable binding to COX17. Since this amino …