Digital Commons Network^™

The Clapper Rail As An Indicator Species Of Estuarine Marsh Health, James N. Novak, Karen F. Gaines, James C. Cumbee Jr., Gary L. Mills, Alejandro Rodriguez-Navarro, Christopher S. Romanek

Faculty Research & Creative Activity

Clapper Rails (Rallus longirostris) can potentially serve as an indicator species of estuarinemarsh health because of their strong site fi delity and predictable diet consisting predominantly of benthic organisms. These feeding habits increase the likelihood of individuals accumulating signifi - cant amounts of contaminants associated with coastal sediments. Moreover, since Clapper Rails are threatened in most of their western range, additional study of the effects of potential toxins on these birds is essential to conservation programs for this species. Here we present techniques (DNA strand breakage, eggshell structure, and human-consumption risk) that can be used to quantify detrimental effects to …

Element Levels In Snakes In South Carolina: Differences Between A Control Site And Exposed Site On The Savannah River Site, J. Burger, S. Murray, K. F. Gaines, James M. Novak, T. Punshon, C. Dixon, M. Gochfeld

Faculty Research & Creative Activity

Levels of 18 elements, including lead, mercury, selenium, and uranium, were examined in three species of snakes from an exposed and reference site on the Department of Energy’s Savannah River Site in South Carolina. We tested the hypotheses that there were no differences as a function of species, and there were no difference between the exposed and control site for blood and muscle (tail) samples for banded water snake (Nerodia fasciata), brown water snake (N. taxispilota) and cottonmouth (Akistrodon piscivorous). The banded water snakes collected were significantly smaller than the other two species. For blood, there were significant species differences …

Profiling Helper T Cell Subset Gene Expression In Deer Mice, Lauren Oko, Bethany Aduddell-Swope, Derall Willis, Robyn Hamor, Teresa A. Coons, Brian Hjelle

Biological Sciences Faculty Publications

BACKGROUND: Deer mice (Peromyscus maniculatus) are the most common mammals in North America and are reservoirs for several zoonotic agents, including Sin Nombre virus (SNV), the principal etiologic agent of hantavirus cardiopulmonary syndrome (HCPS) in North America. Unlike human HCPS patients, SNV-infected deer mice show no overt pathological symptoms, despite the presence of virus in the lungs. A neutralizing IgG antibody response occurs, but the virus establishes a persistent infection. Limitations of detailed analysis of deer mouse immune responses to SNV are the lack of reagents and methods for evaluating such responses.

RESULTS: We developed real-time PCR-based detection assays for …

Predator-Elicited Visual Signal: Why The Turquoise-Browed Motmot Wag-Displays Its Racketed Tail, Troy G. Murphy

Biology Faculty Research

Both sexes of the turquoise-browed motmot (Eumomota superciliosa) perform a wag-display in the presence of predators, whereby their long racketed tail is repeatedly rocked side-to-side in a pendulous fashion. I tested 3 hypotheses for the function of the predator-elicited wag-display: 1) pursuit-deterrent signal, 2) warning alarm signal, and 3) self-preservation alarm signal. These hypotheses were evaluated by testing whether the presence of potential receivers (kin, conspecifics, mate) modified the way in which the wag-display was performed. Data on wag-display were collected when I experimentally presented predators to motmots and when naturally occurring predators were observed at nesting colonies. …

Effect Of In Ovo Exposure To Pcbs And Hg On Clapper Rail Bone Mineral Chemistry From A Contaminated Salt Marsh In Coastal Georgia, Alejandro Rodriquez-Navarro, Christopher S. Romanek, Pedro Alvarez-Lloret, Karen F. Gaines

Faculty Research & Creative Activity

The effect of Hg and PCBs (Aroclor 1268) on bone characteristics was investigated in a population of Clapper Rails (Rallus longirostris) inhabiting contaminated and unimpacted estuarine marsh systems in coastal Georgia. Exposure to contaminants did not affect the length or weight of leg bones, but it significantly altered the chemical composition of the bone. Specifically, bone in the contaminated site had a higher Ca to P, and lower carbonate and acid phosphate content. These characteristics are typical of more mature bone mineral and indicate that toxicants have accelerated bone maturation. FTIR spectroscopy data revealed a dose dependent change in the …

Validation Of Water Flux And Body Composition In Glaucous Gulls (Larus Hyperboreus), Scott A. Shaffer, Gabrielsen, G.W., Verreault, J, Costa, D.P

Faculty Publications, Biological Sciences

Water influx rates (WIR) measured with tritiated water dilution were compared with direct measures of water and energy intake in glaucous gulls (Larus hyperboreus). Total body water (TBW) measured isotopically was also compared with TBW determined by body composition analysis (BCA) of the same birds. Seventeen wild gulls were captured and studied in outdoor enclosures at Ny-Ålesund, Svalbard, in July 2002. Gulls were hand-fed known quantities of Arctic cod (Boreogadus saida) or given water on the basis of one of four experimental treatments: (A) fasting, (B) fish only, (C) water only, or (D) fish and water. Water and energy content …

Winter Profile Of Plasma Sex Steroid Levels In Free-Living Male Western Diamond-Backed Rattlesnakes, Crotalus Atrox (Serpentes: Viperidae), Gordon W. Schuett, Roger A. Repp, Emily N. Taylor, Dale F. Denardo, Ryan L. Earley, Edward A. Van Kirk, William J. Murdoch

Biological Sciences

Recent field studies on the reproductive ecology of western diamond-backed rattlesnakes (Crotalus atrox) from populations in southern Arizona showed significant differences in the concentration of plasma sex steroids (testosterone, T; 5α-dihydrotestosterone, DHT; and 17β-estradiol, E2) throughout the active season (March–October), and peak levels were coincident with the two mating periods (late summer and early spring). There is, however, no information on levels of sex steroids during winter. Similar to most snakes, hibernating individuals of C. atrox are typically inaccessible, but in southern Arizona, where environmental conditions are typically mild during winter, adult males frequently bask at …

A Reaction–Diffusion Analysis Of Energetics In Large Muscle Fibers Secondarily Evolved For Aerobic Locomotor Function, Kristin M. Hardy, Bruce R. Locke, Marilia Da Silva, Stephen T. Kinsey

Biological Sciences

The muscles that power swimming in the blue crab, Callinectes sapidus, grow hypertrophically, such that in juvenile crabs the cell diameters are

Phytoremediation: An Affordable Green Technology For The Clean-Up Of Metal-Contaminated Sites In Sri Lanka, Nishanta Rajakaruna, Kathleen M. Tompkins, Peter G. Pavicevic

Biological Sciences

Phytoremediation, the use of plants to remediate sites contaminated with organic and inorganic pollutants, and phytomining, the technology involved in extracting the pollutants removed for commercial purposes, are rapidly-growing industries with multi-million dollar markets. This solar-driven, green technology is oftenfavored over more conventional methods of clean-up due to its low cost, low impact, and wider public acceptance. In this paper we discuss phytoremediation as a valid alternative for remediating contaminated bodies of soils and water in developing countries like Sri Lanka, where clean-up can often be stalled due to the high costs associated with traditional remediation technologies. While phytoremediation techniques …

The Biology Of Canadian Weeds. 133. Cuscuta Campestris Yuncker, C. Gronovii Willd. Ex Schult., C. Umbrosa Beyr. Ex Hook., C. Epithymum (L.) L. And C. Epilinum Weihe, Mihai Costea, François J. Tardif

Biology Faculty Publications

Cuscuta spp. (dodders) are rootless, holoparasitic herbs with filiform stems attached to the host by numerous haustoria. In Canada, Cuscuta gronovii is the most common native species of the genus followed by Cuscuta campestris and C. umbrosa. Cuscuta epithymum and C. epilinum, both introduced species in Canada, occur occasionally. Infestation by Cuscuta spp. can result in serious yield losses and dodders are listed as noxious weeds in British Columbia, Ontario and Québec, and as restricted weeds in Alberta. These plants have evolved special adaptations to ensure their success: germination occurs late in the season when potential hosts are …

Learning And Cemory In The Cav1.2 Knockout Mouse, Jessica Ann White

Master's Theses and Doctoral Dissertations

Of the many signaling pathways found within neurons, calcium signaling is perhaps the most ubiquitous and versatile. Calcium influx through L-type voltage-gated calcium channels (L-VGCCs) is involved in numerous aspects of neuronal function: activation and regulation of gene transcription, synaptic plasticity, and regulation of neuronal excitability are all modulated by calcium. Because many calcium-related subcellular functions are implicated in the formation and storage of long-term memory, this writer investigated the role of an L-VGCC, CaV1.2, in hippocampus-dependent learning and memory. Utilizing the Cre/loxP gene-targeting system, the CaV1.2 L-VGCC isoform was conditionally deleted in the forebrain of mice. This extensive deletion …

Application Of The ³H-Leucine Incorporation Technique For Quantifying Rates Of Bacterial Secondary Production Associated With Decaying Wetland Plant Litter, Jane E. Gillies

Master's Theses and Doctoral Dissertations

The ³H-leucine incorporation technique for quantifying rates of bacterial production has increased in popularity since its original description for bacterioplankton communities. Previous studies examining ³H-leucine-incorporation conditions for bacterial communities in other habitats have reported a wide range of final leucine concentrations to achieve saturation-level uptake. This study assessed the application of the ³H-leucine-incorporation procedure for measuring bacterial production associated with decaying wetland plant litter. Substrate saturation experiments were conducted on three dates for bacteria colonizing decaying litter of Typha angustifolia, Schoenoplectus validus, and Phragmites australis. Incorporation of 3H-leucine into protein exhibited a biphasic saturation …

A Database For The Study Of Marine Mammal Behavior: Gap Analysis, Data Standardization, And Future Directions, Scott A. Shaffer, D P. Costa

Faculty Publications, Biological Sciences

A relational database that contained published information on the diving behavior and/or movement patterns of marine mammals was compiled to facilitate a modeling effort of the Effects of Sound on the Marine Environment (ESME) program. A total of 448 references from reports, books, and peer-reviewed journal articles were obtained. The metadata describing each animal studied, location of the study, and equipment used were entered into the database as well as empirical data describing the diving behavior and movement patterns of each animal. In total, the database contained 1815 entries from 51 different marine mammal species or subspecies. The majority of …

Interpolation Of Tracking Data In A Fluid Environment, Y Tremblay, Scott A. Shaffer, S L. Fowler, C E. Kuhn, B I. Mcdonald, M J. Weise, C -A Bost, H Weimerskirch, D E. Crocker, M E. Goebel, D P. Costa

Faculty Publications, Biological Sciences

Interpolation of geolocation or Argos tracking data is a necessity for habitat use analyses of marine vertebrates. In a fluid marine environment, characterized by curvilinear structures, linearly interpolated track data are not realistic. Based on these two facts, we interpolated tracking data from albatrosses, penguins, boobies, sea lions, fur seals and elephant seals using six mathematical algorithms. Given their popularity in mathematical computing, we chose Bézier, hermite and cubic splines, in addition to a commonly used linear algorithm to interpolate data. Performance of interpolation methods was compared with different temporal resolutions representative of the less-precise geolocation and the more-precise Argos …

The Rotavirus Enterotoxin Nsp4 Directly Interacts With The Caveolar Structural Protein Caveolin-1, Rebecca D. Parr, Stephen M. Storey, Deanne M. Mitchell, Avery Mcintosh, Minglong Zhou, Kiran D. Mir, Judith M. Ball

Faculty Publications

Rotavirus nonstructural protein 4 (NSP4) is known to function as an intracellular receptor at the endoplasmic reticulum (ER) critical to viral morphogenesis and is the first characterized viral enterotoxin. Exogenously added NSP4 induces diarrhea in rodent pups and stimulates secretory chloride currents across intestinal segments as measured in Ussing chambers. Circular dichroism studies further reveal that intact NSP4 and the enterotoxic peptide (NSP4_114-135) that is located within the extended, C-terminal amphipathic helix preferentially interact with caveola-like model membranes. We now show colocalization of NSP4 and caveolin-1 in NSP4-transfected and rotavirus-infected mammalian cells in reticular structures surrounding the nucleus …

Terpenes And Carbohydrate Source Influence Rumen Fermentation, Digestibility, Intake, And Preference In Sheep, J. J. Villalba, F. D. Provenza, K. C. Olson

Green Canyon Environmental Research Area, Logan Utah

We hypothesized that toxins and nutrients in foods interact to influence foraging behavior by herbivores. Based on this hypothesis we predicted that 1) terpenes in big sagebrush (Artemisia tridentata) influence intake and preference in sheep for diets varying in sources of nonstructural (barley grain) and structural (sugar beet pulp) carbohydrates, and 2) these effects are due to the differential effects of terpenes on fermentation products and apparent digestibility of each class of carbohydrates. Lambs were fed 2 isoenergetic and isonitrogenous diets with varying proportions of the same ingredients (beet pulp- and barley grain-based diet) or offered a choice between the …

Trehalose Uptake Through P2x7 Purinergic Channels Provides Dehydration Protection, Gloria D. Elliott, Jennifer L. Cusick, Xiang-Hong Liu, Michael A. Menze, Trudy Witt, Steven C. Hand, Mehmet Toner

Faculty Research & Creative Activity

The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors (Steinberg et al., 1987; Sung et al., 1985), and with sustained application of ATP it has been shown that dyes as large as 831 daltons can permeate the cell membrane (Steinberg et al, 1987). The current study explores the kinetics of loading α,α-trehalose (342 daltons) into ATP stimulated J774.A1 cells, which are known to express the purinergic P2X7 receptor (Steinberg et al., 1987). Cells that were incubated at 37 ̊C in a 50 mM phosphate buffer (pH 7.0) …

Sequence Analysis Of Nuclear Rdna Of Anastrepha Suspensa, Ann H. Fritz

Faculty Research & Creative Activity

No abstract provided.

Fungal Spore Dispersal By The Eastern Box Turtle (Terrapene Carolina Carolina), S. C. Jones, W. J. Jordan, Scott J. Meiners, A. N. Miller, A. S. Methven

Faculty Research & Creative Activity

Although spores from most macrofungi are wind- or water-dispersed, dispersal may also occur via biotic vectors. The Eastern box turtle (Terrapene carolina carolina) is a facultative mycovore that may play an important role in fungal spore dispersal although, to date, no information exists on fungi occurring in fecal samples of box turtles or on the ecological significance of box turtles as spore dispersal vectors. Consequently, a study of the potential for Eastern box turtles to act as vectors for spore dispersal was initiated by capturing wild turtles and collecting fecal samples. Serial dilutions from fecal samples were made to enumerate …

Eastern Bluebirds Eject Brown-Headed Cowbird Eggs, Brian Peer, Lyndon Hawkins, Edwin Steinke, Patricia Bollinger, Eric Bollinger

Faculty Research & Creative Activity

The relationship between the Brownheaded Cowbird (Molothrus ater) and its cavitynesting hosts has received little attention because of the assumption that cowbirds rarely parasitize these hosts. We tested the Eastern Bluebird (Sialia sialis), a host that is sometimes heavily parasitized by cowbirds, for egg ejection behavior. Bluebirds ejected 65% of experimentally added cowbird eggs (n = 20), but ejected no experimentally added conspecific eggs (n = 66). This suggests that cowbird parasitism, not conspecific brood parasitism, is the selective pressure responsible for egg ejection in this species. This level of rejection may be conservative because bluebirds nest in dark cavities, …

Eastern Bluebirds Eject Brown-Headed Cowbird Eggs, Brian D. Peer, Lyndon R. Hawkins, Edwin P. Steinke, Patricia Blair Bollinger, Eric K. Bollinger

Faculty Research & Creative Activity

The relationship between the Brownheaded Cowbird (Molothrus ater) and its cavitynesting hosts has received little attention because of the assumption that cowbirds rarely parasitize these hosts. We tested the Eastern Bluebird (Sialia sialis), a host that is sometimes heavily parasitized by cowbirds, for egg ejection behavior. Bluebirds ejected 65% of experimentally added cowbird eggs (n = 20), but ejected no experimentally added conspecific eggs (n = 66). This suggests that cowbird parasitism, not conspecific brood parasitism, is the selective pressure responsible for egg ejection in this species. This level of rejection may be conservative because bluebirds nest in dark cavities, …

Fossil History Of Billfishes (Xiphioidei), Harry L. Fierstine

Biological Sciences

This review characterizes each of the five extinct and extant families of billfishes and discusses the relevant literature. Families include: Hemingwayidae (monotypic; late Paleocene, Turkmenistan), Blochiidae (1 genus, 2 species; middle Eocene, Italy), Palaeorhynchidae (4 genera, ca. 22 species; early Eocene-possibly early Miocene, Europe, Iran, Russia, U.S.A.), Xiphiidae that includes the subfamilies Xiphiorhynchinae (1 genus, 9 species; early Eocene-late Oligocene, Egypt, Europe, U.S.A.) and Xiphiinae (monotypic; middle Miocene-present, world-wide temperate- tropical seas), and the Istiophoridae (3 genera, 13 species; middle Miocene-present; world-wide temperate-tropical seas). Billfishes first appeared in the region of the ancient Tethys Sea (or Paratethys), except for the …

Using Latent Effects To Determine The Ecological Importance Of Dissolved Organic Matter To Marine Invertebrates, Dean E. Wendt, Collin H. Johnson

Biological Sciences

The uptake and utilization of dissolved organic matter (DOM) by marine invertebrates is a field that has received significant attention over the past 100 years. Although it is well established that DOM is taken up by marine invertebrates, the extent to which it contributes to an animal's survival, growth, and reproduction (that is, the ecological benefits) remains largely unknown. Previous work seeking to demonstrate the putative ecological benefits of DOM uptake have examined them within a single life stage of an animal. Moreover, most of the benefits are demonstrated through indirect approaches by examining (1) mass balance, or (2) making …

Factors That Influence Elastomeric Coating Performance: The Effect Of Coating Thickness On Basal Plate Morphology, Growth And Critical Removal Stress Of The Barnacle Balanus Amphitrite, D. E. Wendt, G. L. Kowalke, J. Kim, I. L. Singer

Biological Sciences

Silicone coatings are currently the most effective non-toxic fouling release surfaces. Understanding the mechanisms that contribute to the performance of silicone coatings is necessary to further improve their design. The objective of this study was to examine the effect of coating thickness on basal plate morphology, growth, and critical removal stress of the barnacle Balanus amphitrite. Barnacles were grown on silicone coatings of three thicknesses (0.2, 0.5 and 2 mm). Atypical (“cupped”) basal plate morphology was observed on all surfaces, although there was no relationship between coating thickness and i) the proportion of individuals with the atypical morphology, or …

Lassen Volcanic National Park Small Vertebrate Inventory Project: Preliminary Results 2000-2004, John D. Perrine

Biological Sciences

Biologists at Lassen Volcanic National Park in northern California conducted an inventory of non-flying small mammals, amphibians and reptiles from 2000 through 2004. The four primary habitat types in the park (brush, forest, riparian and subalpine) were each sampled at one high elevation and one mid-elevation site in the park. Each of the eight sites was sampled for four six-day periods between June and September each year using a combination of pitfall, Sherman and Tomahawk traps, coverboards and unbaited automatic camera stations. This effort yielded 12,150 captures and 776 photo detections. A total of 649 voucher specimens, representing 19 mammal, …

The Ball Lightning Conundrum, William D. Stansfield

Biological Sciences

The article focuses on the issue regarding the existence of ball lightning. The phenomenon regarding its existence is a reality accepted by most scientists, but how it is formed and maintained has yet to be fully explained. According to the author the rarity of ball lightning and the ease with which some of its characteristics can be confused with other Unidentified Flying Objects (UFO) that makes it unlikely to be the source of all but a few UFO reports.

The Enigma Of Coral Castle, William D. Stansfield

Biological Sciences

The article discusses the mystery of how Coral Castle near Miami, Florida was built. It is believed that the castle was built by a single man using only simple hand tools. The man who said to built the castle was Latvia-born Edward Leedskalnin. How he supported himself and paid for the land and tools for building a castle is an important part of the mystery.

Conservation Medicine On The Galápagos Islands: Partnerships Among Behavioral, Population, And Veterinary Scientists, Patricia Parker, Noah Whiteman, R. Miller

Biology Department Faculty Works

No abstract provided.

The Effect Of Prey Availability On Metabolism And Activity In The Tarantula Phormictopus Cancerides (Araneae: Theraphosidae), Benjamin Nathan Philip

Master's Theses and Doctoral Dissertations

This study examined the effect of different periods of food deprivation on resting metabolic rates (RMR) and foraging activities in tarantulas (Phormictopus cancerides). Juvenile tarantulas were separated into two feeding groups and fed once either every 5 or 30 days. Monthly feeding trials were preceded by RMR measurements. During feeding trials I compared differences between the two groups in (1) prey capture rates, (2) time to prey capture, (3) locomotory activity, and (4) the predator’s prey detection distance. RMRs increased for the well-fed group but remained consistent for individuals, fed only once a month. Time to prey capture …

The Influence Of Woodlot Size And Location In Suburban And Rural Matrices On Bird Species Richness And Individual Abundance, Neil Allen Chartier

Master's Theses and Doctoral Dissertations

This study assessed the influence of woodlot area and matrix composition on bird species richness and individual abundance. Bird surveys were conducted in winter 2004 and 2005 and spring 2005. Woodlot area and landscape composition were analyzed using GIS software. In winter, resident species richness and abundance increased as landscape diversity increased, whereas in spring, resident species richness decreased with increased landscape openness and abundance increased as woodlot area increased. Spring migrant species richness increased with increased landscape openness, and abundance decreased as woodlot area increased. In winter, Tufted Titmice were more likely to be present in smaller woodlots, whereas …