Digital Commons Network^™

Clapper Rails As Indicators Of Mercury And Pcb Bioavailability In A Georgia Saltmarsh System, James C. Cumbee Jr., Karen F. Gaines, Gary L. Mills, N. Garvin, Warren L. Stephens Jr., James N. Novak, I. L. Brisbin Jr.

Faculty Research & Creative Activity

Clapper rails (Rallus longirostris) were used as an indicator species of estuarine marsh habitat quality because of their strong site fidelity and predictable diet consisting of mostly benthic organisms. Mercury (Hg) and the polychlorinated biphenyl (PCB) Aroclor 1268 concentrations were determined for sediments, crabs, as well as clapper rail adults and chicks collected from salt marshes associated with the LCP Superfund site in Brunswick, Georgia. Home ranges were established for adult rails, and sediment and crab samples were taken from each individual’s range. The study was designed to minimize the spatial variability associated with trophic transfer studies by choosing an …

Exposure And Exposure Modeling, Karen F. Gaines, T. E. Chow, S. A. Dyer

Faculty Research & Creative Activity

Exposure to contaminants in the environment is quantified through the ecological risk assessment (ERA) process which provides a framework for the development and implementation of environmental management decisions. The ERA uses available toxicological and ecological information to estimate the probability of occurrence for a specified undesired ecological event or endpoint. The level for these endpoints depends on the objectives and the constraints imposed upon the risk assessment process; therefore, multiple endpoints at different scales may be necessary. ERAs Ecotoxicology | Exposure and Exposure Assessment 1527Author's personal copy often rely on the link between these undesired endpoints to a threshold of …

A Fossil Skull Of The Extant Blue Marlin (Makaira Nigricans Lacapède, 1802) From The Late Miocene Of Orange County, California, Harry L. Fierstine

Biological Sciences

A nearly complete fossil skull, including the rostrum, of blue marlin, Makaira nigricans Lacapède, 1802 (Perciformes: Xiphioidei: Istiophoridae), was collected from the Oso Member (latest Miocene) of the Capistrano Formation, Mission Viejo, Orange County, California. The specimen is compared with extant and fossil istiophorids, and 19 of its 20 morphological variables are within the range of values observed for extant M. nigricans, whereas only 13 or less variables are within the observed range of other extant istiophorids. Because extant M. nigricans usually inhabits a water column with a height of about 200 m or more and is the most …

High Latitude Changes In Ice Dynamics And Their Impact On Polar Marine Ecosystems, Mark A. Moline, Nina J. Karnovsky, Zachary Brown, George J. Divoky, Thomas K. Frazer, Charles A. Jacoby, Joseph J. Torres, William R. Fraser

Biological Sciences

Polar regions have experienced significant warming in recent decades. Warming has been most pronounced across the Arctic Ocean Basin and along the Antarctic Peninsula, with significant decreases in the extent and seasonal duration of sea ice. Rapid retreat of glaciers and disintegration of ice sheets have also been documented. The rate of warming is increasing and is predicted to continue well into the current century, with continued impacts on ice dynamics. Climate-mediated changes in ice dynamics are a concern as ice serves as primary habitat for marine organisms central to the food webs of these regions. Changes in the timing …

An Automated Temperature-Based Option For Estimating Surface Activity And Refuge Use Patterns In Free-Ranging Animals, J R. Davis, E N. Taylor, D F. Denardo

Biological Sciences

Accurately assessing free-ranging animals’ patterns of surface activity and refuge use is critical, yet fundamentally challenging for biologists and wildlife managers. We evaluate the accuracy of an automated technique—temperature-based activity estimation (TBAE)—in estimating surface activity and refuge use patterns of two sympatric reptiles, the western diamond-backed rattlesnake (Crotalus atrox) and the Gila monster (Heloderma suspectum) in the Sonoran Desert. TBAE derived from a comparison of body temperature to shaded air temperature was effective in estimating the overall percent surface activity for both rattlesnakes (observed surface activity 51.8%, TBAE estimated surface activity 48.2%) and Gila monsters (observed …

The Phenotypic Plasticity Of Death Valley's Pupfish: Desert Fish Are Revealing How The Environment Alters Development To Modify Body Shape And Behavior, Sean C. Lema

Biological Sciences

Death Valley seems an unlikely spot to go fishing. Nonetheless, seven species of pupfish survive in North America's lowest, hottest spot as remnants from the cooler, damper Pleistocene Epoch. For the most part, these species exist in isolation and have been left to adapt to minute details of their local environment—sort of the fish version of Darwin's finches. Surprisingly, however, even within a species, when environmental variables such as water temperature or food supply vary, morphological changes are evident within a few generations. This phenotypic plasticity calls into question not only environmental management practices for species preservation but also just …

The Dynamic Evolutionary History Of The Bananaquit (Coereba Flaveola) In The Caribbean Revealed By A Multigene Analysis, Eva Bellemain, Eldredge Bermingham, Robert Ricklefs

Biology Department Faculty Works

BackgroundThe bananaquit (Coereba flaveola) is a small nectivorous and frugivorous emberizine bird (order Passeriformes) that is an abundant resident throughout the Caribbean region. We used multi-gene analyses to investigate the evolutionary history of this species throughout its distribution in the West Indies and in South and Middle America. We sequenced six mitochondrial genes (3744 base pairs) and three nuclear genes (2049 base pairs) for forty-four bananaquits and three outgroup species. We infer the ancestral area of the present-day bananaquit populations, report on the species' phylogenetic, biogeographic and evolutionary history, and propose scenarios for its diversification and range expansion.ResultsPhylogenetic concordance between …

The Plant Ontology Database: A Community Resource For Plant Structure And Developmental Stages Controlled Vocabulary And Annotations, Shulamit Avraham, Chih-Wei Tung, Katica Ilic, Pankaj Jaiswal, Elizabeth Kellogg, Susan Mccouch, Anuradha Pujar, Leonore Reiser, Seung Rhee, Martin Sachs, Mary Schaeffer, Lincoln Stein, Peter Stevens, Leszek Vincent, Felipe Zapata, Doreen Ware

Biology Department Faculty Works

The Plant Ontology Consortium (POC, http://www.plantontology.org ) is a collaborative effort among model plant genome databases and plant researchers that aims to create, maintain and facilitate the use of a controlled vocabulary (ontology) for plants. The ontology allows users to ascribe attributes of plant structure (anatomy and morphology) and developmental stages to data types, such as genes and phenotypes, to provide a semantic framework to make meaningful cross-species and database comparisons. The POC builds upon groundbreaking work by the Gene Ontology Consortium (GOC) by adopting and extending the GOC's principles, existing software and database structure. Over the past year, POC …

The Development Of Caching Behavior, Cache Site And Cache Item Defense Tactics, And Cache Site And Cache Item Sharing In Orphaned Fledgling Blue Jays (Cyanocitta Cristata) At A Songbird Rehabilitation Center, Deanna Martinez

Master's Theses and Doctoral Dissertations

One of the primary goals of songbird rehabilitators is to promote controllable factors that increase survivorship in orphaned songbirds upon to release. As caching serves an important function in corvid life, this study sought to determine if this behavior would develop with or without outside input. Eight orphaned blue jays (consisting of both hatchlings and nestlings) were brought into the care of the Bird Center of Washtenaw County, Inc. in Ann Arbor, Michigan. Birds were videotaped and observed for the development of caching behavior, cache item and cache site sharing, and cache defense tactics. Caching behavior and cache defense tactics …

Estimating Maximum Performance: Effects Of Intraindividual Variation, Stephen C. Adolph, Trevor Pickering '05

All HMC Faculty Publications and Research

Researchers often estimate the performance capabilities of animals using a small number of trials per individual. This procedure inevitably underestimates maximum performance, but few studies have examined the magnitude of this effect. In this study we explored the effects of intraindividual variation and individual sample size on the estimation of locomotor performance parameters. We measured sprint speed of the lizard Sceloporus occidentalis at two temperatures (20 degrees C and 35 degrees C), obtaining 20 measurements per individual. Speed did not vary temporally, indicating no training or fatigue effects. About 50% of the overall variation in speed at each temperature was …

Morphology Of The Inner And Peripheral Ear Of The Loricariid Catfish Pterygoplichthys Gibbiceps K., Brian David Rogers

Mahurin Honors College Capstone Experience/Thesis Projects

The morphology of the peripheral and inner ear structures was studied in the loricariid catfish Pterygoplichthys gibbiceps. Specimens (n=6) were preserved in fixative (4% paraformaldehyde, 2% glutaraldehyde in 0.1M phosphate buffer) and dissected for examination of the gross morphology (using light microscopy) and ultrastructure of the auditory sensory epithelia (using scanning electron microscopy). One additional specimen was cleaned in a dermestid beetle colony in order to examine the osteology of the skull. The swim bladder of P. gibbiceps is divided along the midline of the fish into two reduced but equal lobes residing in two laterally oriented bony encapsulations. Immediately …

What Constitutes A Natural Fire Regime? Insight From The Ecology And Distribution Of Coniferous Forest Birds In North America, Richard L. Hutto, Courtney J. Conway, Victoria A. Saab, Jeffrey R. Walters

Biological Sciences Faculty Publications

Bird species that specialize in the use of burned forest conditions can provide insight into the prehistoric fire regimes associated with the forest types that they have occupied over evolutionary time. The nature of their adaptations reflects the specific post-fire conditions that occurred prior to the unnatural influence of humans after European settlement. Specifically, the post-fire conditions, nest site locations, and social systems of two species (Bachman's sparrow [Aimophila aestivalis] and red-cockaded woodpecker [Picoides borealis]) suggest that, prehistorically, a frequent, low-severity fire regime characterized the southeastern pine system in which they evolved. In contrast, the patterns …

Espousing Interactions And Fielding Reactions: Addressing Laypeople's Beliefs About Genetic Determinism, David S. Moore

Pitzer Faculty Publications and Research

Although biologists and philosophers of science generally agree that genes cannot determine the forms of biological and psychological traits, students, journalists, politicians, and other members of the general public nonetheless continue to embrace genetic determinism. This article identifies some of the concerns typically raised by individuals when they first encounter the systems perspective that biologists and philosophers of science now favor over genetic determinism, and uses arguments informed by that perspective to address those concerns. No definitive statements can yet be made about why genetic determinism has proven so resilient in the face of empirical evidence pointing up its deficiencies, …

Prion Protein As An Infectious Agent Of Transmissible Spongiform Encephalopathies, Wioleta Cyranek

ETD Archive

Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative diseases of human, animal and yeast caused by an infectious agent designated prion. The "protein only" hypothesis proposed more than three decades ago suggests that the prions are small, infectious pathogens composed of aggregated, abnormal forms of a protein encoded in the host genome. PrPC plays primary role in neurite outgrowth, synaptic function, oxidative stress defense and long term survival of cerebellar neurons. The key event in prion disease is the conversion of the Îł-helical, cellular isoform of the prion protein to the insoluble, Îø-sheet-rich disease- causing isoform. Aggregation of misfolded prion protein …

Optimization Of The Conditions Necessary To Show Binding Of The Plasmodium Yoelii Rhop-3 Rhoptry Protein To Mouse Erythrocytes, Latoya T. Myrie

ETD Archive

The Plasmodium Rhop-3 rhoptry protein is an erythrocyte binding protein that is secreted into the RBC membrane during merozoite invasion. Anti-Rhop-3 antibodies inhibit merozoite RBC invasion. The C-terminus of the Rhop-3 protein is highly conserved among Plasmodium species and antisera from endemic areas reacts with recombinant C-terminus of Rhop-3. The binding domain of the Rhop-3 protein is hypothesized to be within the C-terminal region of the protein. In the present study I investigated the conditions necessary for binding of the Rhop-3 protein to RBC by expressing recombinant proteins made from partial fragments of the Rhop-3 gene using the vector pDisplayTM. …

A High Throughput Live Transparent Animal Bioassay To Identify Non-Toxic Small Molecules Or Genes That Regulate Vertebrate Fat Metabolism For Obesity Drug Development, Kevin Jones

Department of Biology Faculty Publications

The alarming rise in the obesity epidemic and growing concern for the pathologic consequences of the metabolic syndrome warrant great need for development of obesity-related pharmacotherapeutics. The search for such therapeutics is severely limited by the slow throughput of animal models of obesity. Amenable to placement into a 96 well plate, zebrafish larvae have emerged as one of the highest throughput vertebrate model organisms for performing small molecule screens. A method for visually identifying non-toxic molecular effectors of fat metabolism using a live transparent vertebrate was developed. Given that increased levels of nicotinamide adenine dinucleotide (NAD) via deletion of CD38 …

Measuring Knowledge Of Natural Selection: A Comparison Of The C.I.N.S., An Open-Response Instrument, And An Oral Interview, Ross Nehm, Irvin Sam Schonfeld

Publications and Research

Growing recognition of the central importance of fostering an in-depth understanding of natural selection has, surprisingly, failed to stimulate work on the development and rigorous evaluation of instruments that measure knowledge of it. We used three different methodological tools, the Conceptual Inventory of Natural Selection (CINS), a modified version of Bishop and Anderson's (Bishop and Anderson [1990] Journal of Research in Science Teaching 27: 415-427) open-response test that we call the Open Response Instrument (ORI), and an oral interview derived from both instruments, to measure biology majors' understanding of and alternative conceptions about natural selection. We explored how these instruments …

Combating Amphibian Population Declines: An Investigation Of The Pathophysiology And Possible Treatments Of Batrachochytrium Dendrobatidis, Christopher A. Gaulke

Undergraduate Honors Theses

The fungus Batrachochytrium dendrobatidis has been linked to the decline of amphibians worldwide. Research has been devoted to examining the range of B. dendrobatidis and various physiological aspects of the fungus; however, little is known about how it causes mortality in individuals. Subsequently, we investigated the potential mechanisms of mortality by examining the pathogen's effect on metabolic rate and osmotic balance. In addition, we attempted to develop a field treatment for the pathogen using Itraconazol. Metabolic rates, mass, pathogen symptoms and osmotic concentrations were measured in Rana pipiens and Hyla rigilla. Results suggest symptoms are variable and individuals survive for …

Ontogenetic Changes In Feeding Morphology And Diet In The Chiselmouth Minnow, Acrocheilus Alutaceus, Corrie Murray

Undergraduate Honors Theses

The chiselmouth minnow (Acrocheilus alutaceus) is an herbivorous fish endemic to freshwater systems of the Pacific Northwest and abundant in mid-reaches of the Yakima River, WA. Uncommon in temperate streams, herbivorous fish are ecologically important because they provide a direct link between primary production and fish biomass. Chiselmouth minnows undergo ontogenetic morphological changes as they transform from insectivore to herbivore, the lower jaw becomes flattened, calcified and hardened for scraping algae and the intestine grows long for digestion. The timing of these changes in relation to diet and ecological status are poorly understood. By quantifying changes in jaw …

Rapid Bursts Of Androgen-Binding Protein (Abp) Gene Duplication Occurred Independently In Diverse Mammals, Christina M. Laukaitis, Andreas Heger, Tyler D. Blakely, Pavel Munclinger, Chris P. Ponting, Robert C. Karn

Scholarship and Professional Work - LAS

Background

The draft mouse (Mus musculus) genome sequence revealed an unexpected proliferation of gene duplicates encoding a family of secretoglobin proteins including the androgen-binding protein (ABP) α, β and γ subunits. Further investigation of 14 α-like (Abpa) and 13 β- or γ-like (Abpbg) undisrupted gene sequences revealed a rich diversity of developmental stage-, sex- and tissue-specific expression. Despite these studies, our understanding of the evolution of this gene family remains incomplete. Questions arise from imperfections in the initial mouse genome assembly and a dearth of information about the gene family structure in other rodents …

Plaster Creek Watershed Management Plan, Fishbeck, Thompson, Carr, & Huber, Inc.

Watershed Reports

The quality of Plaster Creek is influenced by many factors, such as human activities within the Plaster Creek Watershed (Watershed), physical and biological characteristics of the natural resources, and the management of those resources. This document provides an overview of these diverse aspects of the Watershed, including detailed information about the sources and causes of watershed pollution, recommendations needed to address impairments, and steps to implement the recommendations.

A Global Assessment Of Freshwater Crab Diversity , Darren Cj Yeo, Pkl Ng, Neil Cumberlidge, Celio Magalhães, Savel Regan Daniels Phd, Martha R. Campos

Journal Articles

No abstract provided.

Mitochondria In Energy-Limited States : Mechanisms That Blunt The Signaling Of Cell Death., Steven Hand, Michael Menze

Faculty and Staff Scholarship

Cellular conditions experienced during energy-limited states – elevated calcium, shifts in cellular adenylate status, compromised mitochondrial membrane potential – are precisely those that trigger, at least in mammals, the mitochondrion to initiate opening of the permeability transition pore, to assemble additional protein release channels, and to release pro-apoptotic factors. These proapototic factors in turn activate initiator and executer caspases. How is activation of mitochondria-based pathways for the signaling of apoptotic and necrotic cell death avoided under conditions of hypoxia, anoxia, diapause, estivation and anhydrobiosis? Functional trade-offs in environmental tolerance may have occurred in parallel with the evolution of diversified pathways …

Display Of An Inedible Prop As A Signal Of Aggression? Adaptive Significance Of Leaf-Display By The Turquoise-Browed Motmot, Eumomota Superciliosa, Troy G. Murphy

Biology Faculty Research

The incorporation of an inedible object (prop) into a behavioral display is rare among birds. Only four avian taxa have been reported to display with a prop, and in all studied species, prop use has been found to play a role in mate acquisition. However, little is known about the context and adaptive significance of prop use by the motmots. Both male and female motmots perform a leaf-display, whereby a leaf is held horizontally in the tip of the bill for an extended period. I collected observational data on leaf-display by the turquoise-browed motmots (Eumomota superciliosa) to investigate …

A History Of The Biology Department, Omer R. Larson

UND Departmental Histories

This departmental history was written on the occasion of the UND Quasquicentennial in 2008.

Molecular Characterization And Related Aspects Of The Innate Immune Response In Ticks, Daniel E. Sonenshine, Wayne L. Hynes

Biological Sciences Faculty Publications

Compared to insects, little is known about innate immunity in ticks. This chapter addresses the molecular processes that recognize non-self and the cellular and molecular processes mobilized to phagocytose, engulf, inhibit or kill invaders. We discuss the receptors that recognize pathogen associated molecular patterns (PAMPs) and the putative up-regulation of regulatory cascades that lead, ultimately, to cellular or molecular responses. We describe the molecular events that activate the cellular processes and the array of humoral factors that are mobilized against invading organisms, including antimicrobial peptides, proteases and protease inhibitors, lectins, coagulation factors and others. Special attention is directed to the …

Structure-Function Analysis Of The Drosophila Stubble Type Ii Transmembrane Serine Protease, Rachel Morgan

Electronic Theses and Dissertations

Hormonally-triggered regulatory hierarchies play a major role in organismal development. Disruption of a single member of such a hierarchy can lead to irregular development and disease. Therefore, knowledge of the members involved and the mechanisms controlling signaling through such pathways is of great importance in understanding how resulting developmental defects occur. Type II transmembrane serine proteases (TTSPs) make up a family of cell surface-associated proteases that play important roles in the development and homeostasis of a number of mammalian tissues. Aberrant expression of TTSPs is linked to several human disorders, including deafness, heart and respiratory disease and cancer. However, the …

Tmdl Bioassessment Sampling Of Benthic Macroinvertebrates For Lake Jesup And Lake Seminary, Gloria Eby

Electronic Theses and Dissertations

The objective of this study was to obtain a bioassessment using benthic macroinvertebrates to meet TMDL (Total Maximum Daily Load) criteria for an oligotrophic (Lake Seminary) and an eutrophic (Lake Jesup) freshwater system in Seminole County, Florida. Monthly sampling of the benthic macroinverterbrate communities provided important biological data necessary to construct TMDL protocol and trophic state. Since macroinvertebrates are near the base of the food chain, they not only provide a critical role in the natural flow of energy and cycling of nutrients through the food web, but also provide a good indication of water quality by their presence and …

Cis-Regulatory Analysis Of The Pigment Cell Differentiation Gene Polyketide Synthase, David Rogers

Electronic Theses and Dissertations

The analysis of Gene Regulatory Networks (GRNs) is essential to understanding the complete process of embryo development. Elucidating every gene regulatory circuit from maternal regulatory inputs all the way to the activation of differentiation gene batteries is an important step in increasing our understanding of developmental biology. In this work I study the cis-regulatory architecture of a pigment cell differentiation gene, polyketide synthase (SpPks) in the sea urchin Strongylocentrotus purpuratus. SpPks encodes an enzyme that is responsible for the biosynthesis of the sea urchin pigment echinochrome in larval pigment cells. The analysis of the promoter of a differentiation gene will …

Diet And Sexual Dimorphism Of The Desert Iguana, Dipsosaurus Dorsalis, From Sonora, Mexico, C. J. Dibble, Geoffrey R. Smith, J. A. Lemos-Espinal

Faculty Publications

No abstract provided.