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Nona-Arginine Peptides Facilitate Cellular Entry Of Semiconductor Nanocrystals: Mechanisms Of Uptake, Yi Xu

Masters Theses

"Luminescent semiconductor quantum dots (QDs) have recently been used for delivering and monitoring biomolecules, such as drugs and proteins. However, QDs alone have a very low efficiency of transport across the plasma membrane. In order to increase the efficiency of QD delivery, synthetic nona-arginine (sR9) was used, a cell penetrating peptide, to facilitate uptake. Data demonstrated that sR9 could significantly increase the cellular uptake of QDs by noncovalent binding between QDs and sR9. Furthermore, the mechanisms of QD/sR9 cellular internalization were investigated. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/sR9, indicating that internalization is an energy-dependent process. …

Spatial And Temporal Variability Of Macroinvertyebrate Communities In Vernal Pools On The Coastal Plain Of Virginia, Shrijeeta Ganguly

Theses and Dissertations

Vernal pools are often defined as seasonal pools that typically are inundated beginning in the winter and then drying out completely in summer. Though evidence of spatial and temporal variability in the macroinvertebrate communities of vernal pools has been found in previous studies, it has not been studied extensively. The primary objective of this study was to determine the extent of variability in the macroinvertebrate communities within vernal pools closely situated in a forested landscape. An effort was made to explain this variability with respect to certain physiochemical environmental variables of the pools. Significant variability was observed in the macroinvertebrate …

Unique Contractile And Structural Protein Expression In Dog Ileal Inner Circular Smooth Muscle, Thomas J. Eddinger

Biological Sciences Faculty Research and Publications

This study was designed to test the hypothesis that there is heterogeneous expression of contractile and structural proteins between the smooth muscle cells (SMCs) in the inner and outer circular muscle (ICM and OCM) layers of the ileum. Immunohistochemical staining and quantitation of fresh frozen sections of the dog ileum was performed using protein specific antibodies. Smooth muscle (SM) SMA myosin heavy chain (MHC), α- and γ-SM actin, and vinculin all show greater expression in the ICM relative to the OCM. SMB MHC and fibronectin show the opposite pattern, with greater expression in the OCM relative to the ICM. Differences …

Great Curassow And Crested Guan Near Ciudad Victoria, Tamaulipas, Mexico, Timothy Brush

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Great Curassow (Crax rubra) and Crested Guan (Penelope purpurascens) were rediscovered in the Ciudad Victoria area of southwestern Tamaulipas, Mexico for the first time since 1909. Birds were found in low elevation canyons containing permanent running water, with riparian forest dominated by Montezuma bald-cypress (Taxodium mucronatum) and steep scrub-covered slopes. These records represent the northernmost known points of the ranges of both species. Additional field work will be needed to determine whether these small populations are isolated from the nearest known populations in the El Cielo/Gómez Farías area of southern Tamaulipas.

A Comparative Study Of Larval Gene Expression Between A Paedomorphic And Metamorphic Species Of Ambystomatid Salamander, Meredith A. Boley

University of Kentucky Master's Theses

Ambystoma tigrinum undergoes an obligatory metamorphosis while A. mexicanum fails to metamorphose and exhibits paedomorphosis. While it is clear that salamander paedomorphosis is associated with genetic changes that delay developmental timing, it is not clear when and how these changes manifest during development. It is possible that paedomorphic and metamorphic larvae show equivalent patterns of developmental until late in the larval period, when brain regions become competent to stimulate the release of metamorphic hormones. To test this hypothesis, I compared gene expression patterns between the brains of A. mexicanum and A. t. tigrinum larvae. In support of the developmental equivalence …

Furthering Pharmacological And Physiological Assessment Of The Glutamatergic Receptors At The Drosophila Neuromuscular Junction, Junyoung Lee

University of Kentucky Master's Theses

Drosophila larval neuromuscular junctions (NMJs) serve as a model for synaptic physiology. The molecular sequence of the postsynaptic glutamate receptors has been described; however, the pharmacological profile has not been fully elucidated. Despite the postsynaptic molecular sequence used to classify the receptors as a kainate subtype, they do not respond pharmacologically as such. Kainate does not depolarize the muscle, but dampens evoked EPSP amplitudes. Quantal responses show a decreased amplitude and area under the voltage curve indicative of reduced postsynaptic receptor sensitivity to glutamate transmission. ATPA, a kainate receptor agonist, did not mimic kainate’s action. The metabotropic glutamate receptor agonist …

Coiled-Coil-Mediated Dimerization Is Not Required For Myosin Vi To Stabilize Actin During Spermatid Individualization In Drosophila Melanogaster, Tatsuhiko Nogushi, Deborah J. Frank, Mamiko Isaji, Kathryn G. Miller

Biology Faculty Research

Myosin VI is a pointed-end-directed actin motor that is thought to function as both a transporter of cargoes and an anchor, capable of binding cellular components to actin for long periods. Dimerization via a predicted coiled coil was hypothesized to regulate activity and motor properties. However, the importance of the coiled-coil sequence has not been tested in vivo. We used myosin VI's well-defined role in actin stabilization during Drosophila spermatid individualization to test the importance in vivo of the predicted coiled coil. If myosin VI functions as a dimer, a forced dimer should fully rescue myosin VI loss of function …

Evolutionary Time For Dispersal Limits The Extent But Not The Occupancy Of Species’ Potential Ranges In The Tropical Plant Genus Psychotria (Rubiaceae), John R. Paul, Cynthia Morton, Charlotte M. Taylor, Stephen J. Tonsor

Biology Faculty Publications

Explaining the diversity in geographic range sizes among species is a central goal of ecological and evolutionary studies. We tested species age as an explanation of range size variation within a group of understory shrubs in the Neotropics (Psychotria subgenus Psychotria, Rubiaceae). We distinguish between range occupancy (filling an occupied area) and range extent (maximum distances dispersed). We used Bayesian relaxed-clock dating of molecular sequence data to estimate the relative age of species, and we used species distribution modeling to predict species' potential ranges. If the range sizes of species are limited by time for dispersal, we hypothesize that older …

Virus-Encoded Homologs Of Cellular Interleukin-10 And Their Control Of Host Immune Function, Barry Slobedman, Peter A. Barry, Juliet V. Spencer, Selmir Avdic, Allison Abendroth

Biology Faculty Publications

No abstract provided.

Ngasp - The Nematode Genome Annotation Assessment Project, Avril Coghlan, Tristan J. Fiedler, Sheldon J. Mckay, Paul R. Flicek, Todd W. Harris, Darin Blasiar, Lincoln D. Stein

Biomedical Engineering and Sciences Faculty Publications

While the C. elegans genome is extensively annotated, relatively little information is available for other Caenorhabditis species. The nematode genome annotation assessment project (nGASP) was launched to objectively assess the accuracy of protein-coding gene prediction software in C. elegans, and to apply this knowledge to the annotation of the genomes of four additional Caenorhabditis species and other nematodes. Seventeen groups worldwide participated in nGASP, and submitted 47 prediction sets across 10 Mb of the C. elegans genome. Predictions were compared to reference gene sets consisting of confirmed or manually curated gene models from WormBase. Results: The most accurate gene-finders were …

Ecology Of The Evening Bat (Nycticeius Humeralis) At The Northern Edge Of The Range, Olivia Maya Munzer

Master's Theses and Doctoral Dissertations

Only a single colony of evening bats (Nycticeius humeralis) is known to occur in Michigan, and it is the northernmost colony on the North American continent. In mid-May, only female evening bats migrate to Michigan to raise their pups, and in late August, evening bats migrate to southern portions of their range, where they are ubiquitous. I studied the roosting and dietary ecology of the evening bat in southeastern Michigan. Evening bats roosted in cavities, crevices, and under exfoliating bark of older trees located in a bottomland forest, interlaced with waterways and surrounded by agricultural fields. These habitats …

Changes In Biotic And Abiotic Processes Following Mangrove Clearing, Elise Granek, Benjamin I. Ruttenberg

Biological Sciences

Mangrove forests, important tropical coastal habitats, are in decline worldwide primarily due to removal by humans. Changes to mangrove systems can alter ecosystem properties through direct effects on abiotic factors such as temperature, light and nutrient supply or through changes in biotic factors such as primary productivity or species composition. Despite the importance of mangroves as transitional habitats between land and sea, little research has examined changes that occur when they are cleared. We examined changes in a number of biotic and abiotic factors following the anthropogenic removal of red mangroves (Rhizophora mangle) in the Panamanian Caribbean, including …

Clustering Neural Spike Trains With Transient Responses, John D. Hunter, Jianhong Wu, John Milton

WM Keck Science Faculty Papers

The detection of transient responses, i.e. nonstationarities, that arise in a varying and small fraction of the total number of neural spike trains recorded from chronically implanted multielectrode grids becomes increasingly difficult as the number of electrodes grows. This paper presents a novel application of an unsupervised neural network for clustering neural spike trains with transient responses. This network is constructed by incorporating projective clustering into an adaptive resonance type neural network (ART) architecture resulting in a PART neural network. Since comparisons are made between inputs and learned patterns using only a subset of the total number of available dimensions, …

Production Of Marine Trematode Cercariae: A Potentially Overlooked Path Of Energy Flow In Benthic Systems, David W. Thieltges, Xavier De Montaudouin, Brian L. Fredensborg, K. Thomas Jensen, Janet Koprivnikar, Robert Poulin

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Parasites, in particular trematodes, are unseen but ubiquitous components of marine intertidal ecosystems. Although parasites are known to affect population dynamics and food web structure, their potential function as an unrecognized path of energy flow in these ecosystems is yet to be quantified. We use published data on rates at which trematodes produce free-swimming infective larvae (cercariae) that are released from their gastropod intermediate hosts to investigate patterns in cercarial output as a function of different variables, and to calculate the annual production of cercariae in different marine benthic systems. Across 18 trematode species, cercarial output (no. cercariae shed snail–1 …

Trichomycetes From Lentic And Lotic Aquatic Habitats In Ontario, Canada, D. B. Strongman, Merlin M. White

Biology Faculty Publications and Presentations

Fungi and protists make up an ecological group, trichomycetes, that inhabit the guts of invertebrates, mostly aquatic insects. Trichomycetes are reported herein from arthropods collected in lotic habitats (fast flowing streams) and lentic environments (ponds, ditches, seeps, and lakes) from 11 sites in Algonquin Park and 6 other sites in Ontario, Canada. Thirty-two trichomycete species were recovered, including 7 new species: Legeriomyces algonquinensis, Legeriosimilis leptocerci, Legeriosimilis whitneyi and Paramoebidium umbonatum are described from mayfly nymphs (Ephemeroptera); Pennella digitata and Glotzia incilis from black fly and midge larvae (Diptera), respectively; and Arundinula opeongoensis from a crayfish (Crustacea). Legeriomyces rarus Lichtw. & …

Effects Of Olfactory And Visual Predators On Nest Success And Nest-Site Selection Of Waterfowl In North Dakota, Jennifer Borgo

Green Canyon Environmental Research Area, Logan Utah

No abstract provided.

Yellow Head Virus: Transmission And Genome Analyses, Hongwei Ma

Dissertations

Yellow head virus (YHV) is an important pathogen to shrimp aquaculture. Among 13 species of naturally YHV-negative crustaceans in the Mississippi coastal area, the daggerblade grass shrimp, Palaemonetes pugio, and the blue crab, Callinectes sapidus, were tested for potential reservoir and carrier hosts of YHV using PCR and real time PCR. The YHV replicated in the daggerblade grass shrimp, and was still detectable on 36 d post-inoculation, causing 8% mortality after injection. However, YHV did not replicate in the blue crab. These data suggest that the daggerblade grass shrimp could act as a reservoir host for YHV. Storage conditions of …

Biological Responses In A Dynamic Bouyant River Plume, Mark A. Moline, Thomas K. Frazer, Robert Chant, Scott Glenn, Charles A. Jacoby, John R. Reinfelder, Jennifer Yost, Meng Zhou, Oscar Schofield

Biological Sciences

Buoyant river plumes are highly dynamic and often characterized by marked physical and chemical gradients that interact to drive biological responses. For example, interactions among factors resulting in algal growth (e.g., nutrient and light availability) and algal loss (e.g., sinking and zooplankton grazing) vary with spatiotemporal changes in physics and chemistry. The nature of these interactions profoundly influences the transfer and transformation of materials carried by the plume, including nutrients and metals. In April 2005, during the Lagrangian Transport and Transformation Experiment (LaTTE), water from the Hudson River recirculated in a nearshore eddy before moving southward to mix with relatively …

An Analysis Of Illegal Bushmeat Availability In Local Restaurants Located In Voi, Kenya, Bridget A. Sutton

Masters Theses & Specialist Projects

The illegal bushmeat harvest has been identified as a reason for declining wildlife populations throughout much of Africa. For many years the trade was thought to exist primarily in Western Africa. The illegal use of bushmeat in Eastern Africa, including Kenya, went undocumented and unstudied. In 2004, the Born Free Foundation published a study which claimed illegal sale of wild game was substantial in butcheries throughout Nairobi, Kenya. In an effort to determine other markets of the commercial trade, the goal of this study was to analyze local restaurants in Voi, Kenya for illegal bushmeat sale. The town of Voi …

Recombination-Based In Vivo Expression Technology Identifies Helicobacter Pylori Genes Important For Host Colonization, Andrea Castillo, Andrew J. Woodruff, Lynn E. Connolly, William E. Sause, Karen M. Ottemann

Biology Faculty Publications

Here we undertook to identify colonization and gastric disease-promoting factors of the human gastric pathogen Helicobacter pylori as genes that were induced in response to the stomach environment. Using recombination-based in vivo expression technology (RIVET), we identified six promoters induced in the host compared to laboratory conditions. Three of these promoters, designated Pivi10, Pivi66, and Pivi77, regulate genes that H. pylori may use to interact with other microbes or the host. Pivi10 likely regulates the mobA, mobB, and mobD genes, which have potential roles in horizontal gene transfer through plasmid mobilization. Pivi66 occurs in the cytotoxin-associated gene pathogenicity island, a …

Dietary Exposure To 2,2´,4,4´-Tetrabromodiphenyl Ether (Pbde-47) Alters Thyroid Status And Thyroid Hormone–Regulated Gene Transcription In The Pituitary And Brain, Sean C. Lema, Jon T. Dickey, Irvin R. Schultz, Penny Swanson

Biological Sciences

No abstract provided.

Integrating Development And Evolution In Psychology: Looking Back, Moving Forward, David S. Moore

Pitzer Faculty Publications and Research

This work is the editorial for a special edition of New Ideas in Psychology titled Integrating Development and Evolution in Psychology.

Distribution Patterns Of Sciurus Niger (Eastern Fox Squirrel) Leaf Nests Within Woodlots Across A Suburban/Urban Landscape, Carmen M. Salsbury

Scholarship and Professional Work - LAS

To determine habitat characteristics that influence Sciurus niger (Eastern Fox Squirrel) abundance and distribution within a suburban/urban landscape in the midwestern United States, I documented the density and placement of fox squirrel leaf nests in 20 woodlots in the Indianapolis metropolitan area, Marion County, IN. The woodlots varied in size (0.94 to 19.5 ha), approximate age, shape, and degree of isolation from other woodlots and suitable squirrel habitat in the surrounding area. Only 8.0% of nests were located in a tree with another nest, and nests were randomly distributed in all but one woodlot, where they were uniformly dispersed. Nest …

Preliminary Analysis Of The Ecology And Geography Of The Asian Nuthatches (Aves: Sittidae), Shaily Menon, Zafar-Ul Islam, Jorge Soberon, A. Townsend Peterson

Peer Reviewed Publications

We explored distributions of Asian nuthatch species in ecological and geographic space using ecological niche modeling based on occurrence data associated with specimens and observations. Nuthatches represent a well-defined clade occurring throughout the Northern Hemisphere, but are most diverse in southern Asia where 15 of the 24 species occur and where the lineage is believed to have evolved. Species richness was focused in a narrow east-west band corresponding to the forested parts of the Himalayas with a maximum number of nine species predicted present in these foci. The distributional predictions have a mid-elevation focus with highest species diversity between 1,000 …

The Ecological Importance Of Severe Wildfires: Some Like It Hot, Richard L. Hutto

Biological Sciences Faculty Publications

Many scientists and forest land managers concur that past fire suppression, grazing, and timber harvesting practices have created unnatural and unhealthy conditions in the dry, ponderosa pine forests of the western United States. Specifically, such forests are said to carry higher fuel loads and experience fires that are more severe than those that occurred historically. It remains unclear, however, how far these generalizations can be extrapolated in time and space, and how well they apply to the more mesic ponderosa pine systems and to other forest systems within the western United States. I use data on the pattern of distribution …

Construction Of A New Model System In Saccharomyces Cerevisa To Study Yopo And Identify Its Targets, Laura Nejedlik

Dissertations

Yersinia species use a type three secretion system (TTSS) to inject Yersinia outer proteins (Yops) into the target cell. The effector YopO is a serine/threonine kinase which upon association with actin becomes active. However, activation of the kinase is not required for actin disruption. YopO also contains a Rho/Rac binding domain, but it is unclear how it affects these proteins.

We have established an expression system in Saccharomyces cerevisiae to study YopO. The expression vector contains a GAL1 promoter for inducible expression of YopO as well as a V5 epitope used for visualization of each Yop. We have shown that …

Monogenic Control Of Sex-Limited Colouration In The Honey Gourami, Trichogaster Chuna, Jack Frankel

Department of Biology Faculty Publications

Of the numerous subtropical and tropical freshwater speciesof  fish,  labyrinth  fish  are  among  the  most  varied  in  bodymarkings and colouration patterns. Four taxonomic families(Belontiidae, Anabantidae, Helostomatidae, and Osphrone-midae)  of  the  suborder  Anabantoidei  comprise  the  ‘tradi-tional’  labyrinth  fishes,  a  group  of  about  80  African  andSoutheast Asian species (Linke 1991).  These Anabantoidsare popular with aquarists due to their interesting reproduc-tive behaviours, with males of most species brooding eggs intheir mouths or in floating bubble nests (Vevers 1980; Linke1991; Axelrod and Vorderwinkler 1995; Mills 2000).  Theyhave also been the focus of genetic, environmental, and mor-phological studies (Sommer 1982; Gosline 1985; Klinkhardtet  al.  1995; …

Adverse Effects Of Chronic Circadian Desynchronization In Animals In A "Challenging" Environment, Fabian Preuss, Tueming Tang, Aaron D. Laposky, Deanna M. Arble, Ali Keshavarzian, Fred W. Turek

Biological Sciences Faculty Research and Publications

Continuous disruption of circadian rhythms, as seen in human shift workers, has been associated with the development of a number of adverse mental and physiological conditions. However, scientific evidence linking circadian disruption to overall health, particularly in animal models, is not well documented. In this study, we have demonstrated that exposing C57BL/6J mice to 12-h phase shifts every 5 days for 3 mo had no effect on body weight or intestinal physiology. However, when animals were further challenged with dextran sodium sulfate to induce colitis, chronic shifting of the light-dark cycle led to a dramatic increase in the progression of …

A Cell Biology Laboratory Exercise To Study Sub-Cellular Organelles In Drosophila, Meghana Tare, Amit Singh

Biology Faculty Publications

The fast-changing scenario of undergraduate education puts emphasis on introducing students to hands-on techniques as part of their laboratory courses. In order to cater to large numbers of students and the time constraints involved with undergraduate level laboratory courses, there is a need for development of experiments that are cost effective and can be completed in a defined time frame. We have devised a laboratory exercise for teaching cell biology using the Drosophila melanogaster model. Drosophila can be reared in a short period of time in a cost effective manner. We used Drosophila tissue to study the sub-cellular organization of …

Evaluation Of Methods For Establishing Vegetation In Created Wetland Mitigation Sites In Eastern Nebraska, Andrew A. Miller

Student Work

Seventeen wetland mitigation sites in eastern Nebraska were evaluated, by vegetative zone, to assess the relative success of active and passive wetland vegetation establishment techniques. For sites with available records of species introduced, 46% of the seeded species and 31% of the transplanted species were successfully established suggesting that the intentional introduction of species (i.e. active methods) provides some degree of success in wetland creation. No significant differences were observed within or among active or passive methods for Species Richness (S), Shannon-Wiener diversity (H′), or the Floristic Quality Index (FQI) (Kruskall-Wallis test P < 0.05). However, while not statistically significant, general trends showed that, in the temporarily flooded zones, seeding resulted in both the highest overall plant diversity and highest FQI (S = 20.2, H′ = 1.74, FQI = 8.99) and, among species with cover values > 0.5%, the lowest percent non-native species (6.2%). …