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Effects Of Small Increases In Corticosterone Levels On Morphology, Immune Function, And Feather Development, Michael W. Butler, Lynda L. Leppert, Alfred M. Dufty Jr.

Biology Faculty Publications and Presentations

Stressors encountered during avian development may affect an individual’s phenotype, including immunocompetence, growth, and feather quality. We examined effects of simulated chronic low-level stress on American kestrel (Falco sparverius) nestlings. Continuous release of corticosterone, a hormone involved in the stress response, can model chronic stress in birds. We implanted 13-d-old males with either corticosterone-filled implants or shams and measured their growth, immune function, and feather coloration.We found no significant differences between groups at the end of the weeklong exposure period in morphometrics (mass, tarsus, wing length, and asymmetry), immunocompetence (cutaneous immunity, heterophil/lymphocyte ratio, and humoral immunity), or feather …

A Search For Genetic Modifiers Responsible For Congenital Heart Disease Variability In The Presence Of Nkx2-5 Haploinsufficiency, Julia Brandeis Winston Honold

All Theses and Dissertations (ETDs)

While a clear heritable risk has been observed for congenital heart disease, there is considerable variation in penetrance and presentation likely due to multiple genetic and environmental risk factors. To identify causative factors and interactions responsible for variability in heart development, greater than 4,200 hearts from Nkx2-5 heterozygous knockout mice have been collected and examined. Nkx2-5+/- mice in the inbred strain background C57Bl/6 frequently have atrial and ventricular septal defects. The incidences are substantially reduced in the Nkx2-5+/- progeny of first-generation: F1) outcrosses to the strains FVB/N or A/J. Defects recur in the second generation: F2) of the F1xF1 intercross …

Leptodactylus Cunicularius, W. R. Heyer, Miriam M. Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus cunicularius are moderately small. The head is longer than wide and the hind limbs are long (Table 1; Heyer and Thompson 2000 provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are internal, not externally expanded. The snout is protruding, not sexually dimorphic. Male forearms are not hypertrophied and males lack asperities on the thumbs and chest. The dorsum is variegated with small, often confluent, spots and blotches. There is a very thin interrupted mid-dorsal light stripe (pinstripe). Usually, there is a noticeable light, irregular, elongate, mid-dorsal blotch in the scapular region. The …

Leptodactylus Syphax, W. R. Heyer, Miriam M. Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus syphax are moderately sized (males 58-83 mm, females 70-90 mm SVL). The head is about as long as wide, but usually is just wider than long. The hind limbs are moderately short (Table 1; Heyar and Thompson 2000 provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are laterally expanded, tan, and not darker than the adjacent throat. The male snout is not spatulate, the snout profile is rounded to obtuse in both sexes. Male arms are hypertrophied during the breeding season in sexually active males. The male thumb has two large, …

Genome Sequence Of The Palaeopolyploid Soybean, Jeremy Schmutz, Steven B. Cannon, Jessica Schlueter, Jianxin Ma, Therese Mitros, William Nelson, David L. Hyten, Qijian Song, Jay J. Thelen, Jianlin Cheng, Dong Xu, Uffe Hellsten, Gregory D. May, Yeisoo Yu, Tetsuya Sakurai, Taishi Umezawa, Madan K. Bhattacharyya, Devinder Sandhu, Babu Valliyodan, Erika Lindquist, Myron Peto, David Grant, Shengqiang Shu, David Goodstein, Kerrie Barry, Montona Futrell-Griggs, Brian Abernathy, Jianchang Du, Zhixi Tian, Liucun Zhu, Navdeep Gill, Trupti Joshi, Marc Libault, Anand Sethuraman, Xue-Cheng Zhang, Kazuo Shinozaki, Henry T. Nguyen, Rod A. Wing, Perry Cregan, James Specht, Jane Grimwood, Dan Rokhsar, Gary Stacey, Randy C. Shoemaker, Scott A. Jackson

Biology Faculty Articles

Soybean (Glycine max) is one of the most important crop plants for seed protein and oil content, and for its capacity to fix atmospheric nitrogen through symbioses with soil-borne microorganisms. We sequenced the 1.1-gigabase genome by a whole-genome shotgun approach and integrated it with physical and high-density genetic maps to create a chromosome-scale draft sequence assembly. We predict 46,430 protein-coding genes, 70% more than Arabidopsis and similar to the poplar genome which, like soybean, is an ancient polyploid (palaeopolyploid). About 78% of the predicted genes occur in chromosome ends, which comprise less than one-half of the genome but …

Domestic Exotics And The Perception Of Invasibility, Qinfeng Guo, Robert Ricklefs

Biology Department Faculty Works

Susceptibility of an area to invasion by exotic species is often judged by the fraction of introduced species in the local biota. However, the degree of invasion, particularly in mainland areas, has often been underestimated because of the exclusion of ‘domestic exotics’ (those introduced to internal units from within the national border) in calculations. Because all introduced species on islands are considered as exotics, this contributes to the perception that islands are more susceptible to invasion than are continental regions. Here, we determine the contribution of domestic exotic species to the degree of invasion (exotic fraction) in mainland areas. We …

Insights From Comparative Analyses Of Aging In Birds And Mammals, Robert Ricklefs

Biology Department Faculty Works

Many laboratory models used in aging research are inappropriate for understanding senescence in mammals, including humans, because of fundamental differences in life history, maintenance in artificial environments, and selection for early aging and high reproductive rate. Comparative studies of senescence in birds and mammals reveal a broad range in rates of aging among a variety of taxa with similar physiology and patterns of development. These comparisons suggest that senescence is a shared property of all vertebrates with determinate growth, that the rate of senescence has been modified by evolution in response to the potential life span allowed by extrinsic mortality …

Genome Sequence Of The Pea Aphid Acyrthosiphon Pisum, The International Aphid Genomics Consortium

Biology Articles and Papers

Aphids are important agricultural pests and also biological models for studies of insect-plant interactions, symbiosis, virus vectoring, and the developmental causes of extreme phenotypic plasticity. Here we present the 464 Mb draft genome assembly of the pea aphid Acyrthosiphon pisum. This first published whole genome sequence of a basal hemimetabolous insect provides an outgroup to the multiple published genomes of holometabolous insects. Pea aphids are host-plant specialists, they can reproduce both sexually and asexually, and they have coevolved with an obligate bacterial symbiont. Here we highlight findings from whole genome analysis that may be related to these unusual biological features. …

A Habitat Model For The Detection Of Two-Lined Salamanders At C. F. Phelps Wildlife Management Area, Fauquier And Culpeper Counties, Virginia, Jay Mcghee, Michael D. Killian

Virginia Journal of Science

Aquatic salamanders represent an important component of Virginia river watersheds, but despite potential declines, few specifics are known about their habitat preferences. We surveyed the habitats of the northern two-lined salamander and collected data on an array of habitat variables associated with the species. We used a logistic regression analysis to develop a model predicting its presence or absence for a given 50m-transect. Our final model incorporated the variation in stream depth and direction of stream flow and accounted for 25% of the variation in our data. We conclude that stream depth variation is an important feature of salamander habitat …

Ecological Opportunity And The Origin Of Adaptive Radiations, J. B. Yoder, E. Clancey, S. Des Roches, J. M. Eastman, L. Gentry, W. Godsoe, T. J. Hagey, D. Jochimsen, B. P. Oswald, J. Robertson, B. Sarver, J. J. Schenk, S. F. Spear, L. J. Harmon

Biology

Ecological opportunity – through entry into a new environment, the origin of a key innovation or extinction of antagonists – is widely thought to link ecological population dynamics to evolutionary diversification. The population-level processes arising from ecological opportunity are well documented under the concept of ecological release. However, there is little consensus as to how these processes promote phenotypic diversification, rapid speciation and adaptive radiation. We propose that ecological opportunity could promote adaptive radiation by generating specific changes to the selective regimes acting on natural populations, both by relaxing effective stabilizing selection and by creating conditions that ultimately generate diversifying …

Thermal Preferences And Distribution Of Northern Michigan Crayfishes, Troy Keller

Faculty Bibliography

Crayfishes function as both ecosystem engineers and keystone species, and they serve as the trophic linkage between benthic organisms and fish predators. Because crayfish behavior, physiology, and growth are controlled by temperature, thermal differences among habitats may have important implications for crayfishes and the ecosystems they inhabit. To investigate the association between crayfish and temperature, we measured thermal preferences of 3 crayfish species in lab experiments (2007), recorded summer water temperatures at 9 sites (July 1-August 9, 2008), and trapped crayfishes at these same sites in northern Michigan. Orconectes rusticus (non-indigenous) preferred nearly 1 °C warmer water (=22 °C) than …

Evidence For Decline In The Unionidae Of The East Branch Rocky River, Ohio, Daniel A. Gouch, Mark S. Lyons, Robert A. Krebs

Biological, Geological, and Environmental Faculty Publications

A survey of freshwater mussels (Mollusca: Bivalvia: Unionidae) was conducted for the East Branch Rocky River, Ohio, during the summer of 2006. Development in this watershed is moderate though rapidly expanding, particularly in the upstream reaches. Perhaps as a consequence, a preliminary search failed to reveal any live mussels in the river in 2003 . The present report is of an extensive survey covering 21 two-hour timed searches for live mussels and shells combined with visual assessments of disturbance and tests for water quality at each site. All searches were visual or by feeling for mussels embedded in the stream …

The Role Of Chmp1 In Drosophila Melanogaster, Meagan E. Valentine

Theses, Dissertations and Capstones

Chmp1A has recently been linked to pancreatic cancer, a leading cause of cancer death in humans. Pancreatic tumors have lowered Chmp1A expression, and it has been described as a tumor suppressor. Chmp1A is also a member of ESCRT III (Endosomal Sorting Complex Required for Transport), a conserved protein complex involved in the degradation and recycling of activated transmembrane receptors. There is a single Chmp1 protein in Drosophila that is homologous to vertebrate Chmp1A; however, Chmp1 hasn’t been studied in Drosophila. The objective of this study was to characterize Chmp1 in Drosophila using gene knockdown and over-expression. We used an …

A Unique Population Of Cave Bears (Carnivora: Ursidae) From The Middle Pleistocene Of Kents Cavern, England, Based On Dental Morphometrics, Donald A. Mcfarlane, Martin Sabol, Joyce Lundberg

WM Keck Science Faculty Papers

The ‘breccia’ stratum from Kents (we follow local tradition in using the form ‘Kents’, without an apostrophe) Cavern, England, has been well known for its rich yield of cave-bear material since excavations began in the mid-19th century. Recent work has established that the bears are of latest MIS 12 or earliest MIS 11 age. A life table based on a collection of 67 molariform teeth is consistent with the use of the cave as a hibernaculum. Univariate and morphological assessment of the teeth shows an unusual range of primitive and more derived characters. Multivariate morphometric analysis of cave-bear teeth from …

Differences In Antibiotic Resistance In Fresh-Water Bacteria From Furman Lake And Feeder Streams, Dylan Richards, Clarissa Graham, Laura Snyder

Furman Lake Restoration Book Gallery

A one page summary by three Furman students describing their research on bacteria found in the Furman Lake.

The Effect Of The Lake Restoration Project On Passerine Bird Diversity, Gina Tong, Brittany Martin, Stephanie Field, Whitney Snow, Whitney Millegan, Chris Cowart

Furman Lake Restoration Book Gallery

A three page summary by six Furman students showing their research results on the lake restoration's effects on passerine bird populations.

Tissue-Diet Discrimination Factors And Turnover Of Stable Carbon And Nitrogen Isotopes In White-Footed Mice (Peromyscus Leucopus), Rachel L. Demots, James M. Novak, Karen F. Gaines, Aaron J. Gregor, Christopher S. Romanek, Daniel A. Soluk

Faculty Research & Creative Activity

Stable isotope analysis has become an increasingly valuable tool in investigating animal ecology. Here we document the turnover rates for carbon in the liver, muscle, and whole blood tissue, as well as the tissue-diet discrimination values for carbon and nitrogen isotopes in the liver, whole blood, muscle, and hair, of the white-footed mouse (Peromyscus leucopus (Rafinesque, 1818)). A 168-day diet-switching experiment was conducted with a laboratory population of white-footed mice. The δ13C values for all tissues deviated less than 1‰ from those of the diet except for whole blood, which had a slightly higher tissue-diet discrimination factor of 1.8‰. All …

Mechanisms Of Apoptosis In Crustacea: What Conditions Induce Versus Suppress Cell Death?, Michael A. Menze, Grady Fortner, Suman Nag, Steven C. Hand

Faculty Research & Creative Activity

Arthropoda is the largest of all animal phyla and includes about 90% of extant species. Our knowledge about regulation of apoptosis in this phylum is largely based on findings for the fruit fly Drosophila melanogaster. Recent work with crustaceans shows that apoptotic proteins, and presumably mechanisms of cell death regulation, are more diverse in arthropods than appreciated based solely on the excellent work with fruit flies. Crustacean homologs exist for many major proteins in the apoptotic networks of mammals and D. melanogaster, but integration of these proteins into the physiology and pathophysiology of crustaceans is far from complete. Whether apoptosis …

The Pneumococcal Serine-Rich Repeat Protein Is An Intra-Species Bacterial Adhesin That Promotes Bacterial Aggregation In Vivo And In Biofilms, Carlos J. Sanchez, Pooja Shivshankar, Kim Stol, Samuel Trakhtenbroit, Paul M. Sullam, Karin Sauer, Peter W.M. Hermans, Carlos J. Orihuela

Biological Sciences Faculty Scholarship

The Pneumococcal serine-rich repeat protein (PsrP) is a pathogenicity island encoded adhesin that has been positively correlated with the ability of Streptococcus pneumoniae to cause invasive disease. Previous studies have shown that PsrP mediates bacterial attachment to Keratin 10 (K10) on the surface of lung cells through amino acids 273-341 located in the Basic Region (BR) domain. In this study we determined that the BR domain of PsrP also mediates an intra-species interaction that promotes the formation of large bacterial aggregates in the nasopharynx and lungs of infected mice as well as in continuous flow-through models of mature biofilms. Using …

Responses Of Bats To Forest Fragmentation In The Mississippi River Alluvial Valley, Arkansas, Usa, Rex E. Medlin Jr., Matthew B. Connior, Karen F. Gaines, Thomas S. Risch

Faculty Research & Creative Activity

Intense conversion of bottomland hardwood forests to rice and soybeans in the Mississippi River Valley of Arkansas has restricted the remaining forest to isolated fragments. Habitat fragmentation has proven to be detrimental to population sustainability of several species, and is the subject of intense study with often species and latitude specific responses. We compared both coarse land area classes and landscape fragmentation metrics from six 30 km × 30 km subsets centered on publicly owned management areas to bat captures obtained from a 2005 population study. Patch density was the strongest predictor of total captures (R 2 = 0.801, p …

Reconstitution Of The Olfactory Epithelium Following Injury In Apoe-Deficient Mice, Britto P. Nathan, Salina Gairhe, Ikemefuna Nwosu, Stephen Clark, Robert G. Struble

Faculty Research & Creative Activity

ApoE, a protein component of lipoproteins, is extensively expressed in the primary olfactory pathway. Because apoE has been shown to play a vital role in nerve repair and remodeling, we hypothesized that apoE expression will increase in the injured olfactory epithelium (OE), and that apoE deficiency in apoE knockout (KO) mice will lead to delayed/incomplete reconstitution of the OE following injury. To directly test this hypothesis, we compared OE regeneration in wild-type (WT) and KO mice following injury induced by intranasal irrigation of Triton X-100. OE was collected at 0, 3, 7, 21, 42, and 56 days post lesion. The …

Differences In Ultrasonic Vocalizations Between Wild And Laboratory California Mice (Peromyscus Californicus), M. C. Kalcounis-Rueppell, R. Petric, J. R. Briggs, C. Carney, M. M. Marshall, J. T. Willse, David O. Ribble, J. P. Crossland

Biology Faculty Research

Background: Ultrasonic vocalizations (USVs) emitted by muroid rodents, including laboratory mice and rats, are used as phenotypic markers in behavioral assays and biomedical research. Interpretation of these USVs depends on understanding the significance of USV production by rodents in the wild. However, there has never been a study of muroid rodent ultrasound function in the wild and comparisons of USVs produced by wild and laboratory rodents are lacking to date. Here, we report the first comparison of wild and captive rodent USVs recorded from the same species, Peromyscus californicus.

Methodology and Principal Findings: We used standard ultrasound recording techniques …

Elephants - A Conservation Overview, Heidi Riddle, Bruce A. Schulte, Ajay Desai, Laura Van Der Meer

Biology Faculty Publications

Loss of habitat is one of the most significant problems facing elephants worldwide, leading to clashes over resources between wildlife and humans where elephants receive the largest part of blame – defined as Human Elephant Conflict (HEC). The sub-Saharan region of Africa contains an approximate population of 500,000 elephants that occupy 37 range countries. The African Elephant (Loxodonta africana) is categorized as Vulnerable in the Red List of Threatened Species; they are listed there as two distinct subspecies: the Savanna Elephant (L.a. africana) and the Forest Elephant (L. a. cyclotis). The Red List of …

Investigating Instructors' Conceptions Of Evolution Through The Development Of The Classroom Test Of Evolutionary Reasoning, Patricia Eileen Palko

Electronic Theses and Dissertations

In order to assess the evolutionary understanding of high school biology teachers, an instrument entitled the Classroom Test of Evolutionary Reasoning (CTER) was developed. Content, response process, relations to another variable (the Conceptual Inventory of Natural Selection, or CINS), and internal structure evidence were compiled. The second version of the CTER was found to have adequate reliability and validity estimates to allow it to continue to be used as a measurement tool for an understanding of evolution. When assessing a sample of teachers (n = 724), the mean score was 6.35 out of a possible 13 points. The sample was …

The Proteomic Response Of The Mussel Congeners Mytilus Gallaprovincialis And M. Trossulus To Acute Heat Stress: Implications For Thermal Tolerance Limits And Metabolic Costs Of Thermal Stress, Lars Tomanek, Marcus J. Zuzow

Biological Sciences

The Mediterranean blue mussel, Mytilus galloprovincialis, an invasive species in California, has displaced the more heat-sensitive native congener, Mytilus trossulus, from its former southern range, possibly due to climate change. By comparing the response of their proteomes to acute heat stress we sought to identify responses common to both species as well as differences that account for greater heat tolerance in the invasive. Mussels were acclimated to 13°C for four weeks and exposed to acute heat stress (24°C, 28°C and 32°C) for 1 h and returned to 13°C to recover for 24 h. Using two-dimensional gel electrophoresis and …

Zooplankton Avoidance Of A Profiled Open-Path Fluorometer, Kelly J. Benoit-Bird, Mark A. Moline, Oscar M. Schofield, Ian C. Robbins, Chad M. Waluk

Biological Sciences

Significant avoidance of acoustically detected zooplankton was observed in response to a profiling instrument package. Avoidance decreased acoustic scattering from zooplankton averaged over the entire profile by more than a factor of 2, while the maximum avoidance decreased zooplankton acoustic scattering by a factor of 15 over the depth of some discrete scattering layers. Experimental manipulation of the profiler and its instruments revealed that an open-path fluorometer was triggering the avoidance. Avoidance occurred at an average of 8 m below the profiler with a range between 2 and 13 m. Effect range was positively correlated with the average attenuation coefficient …

Meeting The Challenges: The Western Section's Role In Developing Tomorrow's Resource Management Professionals, John D. Perrine

Biological Sciences

The following is a slightly edited version of a plenary address delivered at the Western Section annual meeting in January 2009. The plenary theme was “The view from the future: wildlife management challenges and opportunities of the next 100 years.” Key references are listed at the end of the address. Readers will also be interested in the Winter 2009 (Vol. 3, No. 4) issue of The Wildlife Professional, which contains a special section on the preparation and training of future wildlife professionals.

Extracellular Matrix Of The Charophycean Green Algae, Sarah Nelson Kiemle

Dissertations, Master's Theses and Master's Reports - Open

A comprehensive knowledge of cell wallstructure and function throughout the plant kingdom is essential to understanding cell wall evolution. The fundamental understanding of the charophycean green algal cell wall is broadening. The similarities and differences that exist between land plant and algal cell walls provide opportunities to understand plant evolution. A variety of polymers previously associated with higher plants were discovered in the charophycean green algae (CGA), including homogalacturonans, cross-linking glycans, arabinogalactan protein, β-glucans, and cellulose. The cellulose content of CGA cell walls ranged from 6% to 43%, with the higher valuescomparable to that found in the primary cell wall …

The Importance, Effects, And Ethics Of Bird Collecting, Kevin Winker, J. Michael Reed, Patricia Escalante, Robert A. Askins, Carla Cicero, Gerald E. Hough, John Bates

College of Science & Mathematics Departmental Research

No abstract provided.

Catalog Of The Coreidae, Or Leaf-Footed Bugs, Of The New World, Richard J. Packauskas

Fort Hays Studies Series

Studies on the New World Coreidae have languished for more than a century. Neglect of these often large, abundant, and occasionally economically important bugs has been due, I believe, to the lack of means to identify them. Most literature treating the New World Coreidae has been restricted to the North American fauna, except for the recent efforts of Brailovsky (1975 to 2007).