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Phylogenetic Analysis Of Peste Des Petits Ruminants Virus (Pprv) Isolated In Iran Based On Partial Sequence Data From The Fusion (F) Protein Gene, Majid Esmaelizad, Saber Jelokhani-Niaraki, Rohani Kargar-Moakhar Jan 2011

Phylogenetic Analysis Of Peste Des Petits Ruminants Virus (Pprv) Isolated In Iran Based On Partial Sequence Data From The Fusion (F) Protein Gene, Majid Esmaelizad, Saber Jelokhani-Niaraki, Rohani Kargar-Moakhar

Turkish Journal of Biology

In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. The partial nucleotide and predicted amino acid sequence of the Iranian isolate was aligned with the corresponding sequences of 16 previously published F genes. Sequence analysis of the strains showed that the Iranian isolate had the highest degree of homology with the majority of the strains, with the exception of Nigerian isolates …


Effects Of Vesicular Arbuscular Mycorrhiza Glomus Intraradices On Photosynthetic Pigments, Antioxidant Enzymes, Lipid Peroxidation, And Chromium Accumulation In Maize Plants Treated With Chromium, Raziyeh Rahmaty, Jalil Khara Jan 2011

Effects Of Vesicular Arbuscular Mycorrhiza Glomus Intraradices On Photosynthetic Pigments, Antioxidant Enzymes, Lipid Peroxidation, And Chromium Accumulation In Maize Plants Treated With Chromium, Raziyeh Rahmaty, Jalil Khara

Turkish Journal of Biology

Contamination of soil and ground water by chromium (Cr), due to its wide industrial use, has become a serious source of concern over the past decade. In this study a glasshouse experiment was conducted to investigate the effects of the mycorrhizal fungus Glomus intraradices on Cr toxicity in maize plants. Half of the plants were inoculated with the arbuscular mycorrhizal fungus (AMF). Cr was supplied in the form of potassium dichromate at 0.00, 0.10, 0.25, and 0.50 mM through irrigation water in a sand culture. At the end of the experiment, it was observed that Cr had significantly decreased the …


Biochemical Factors Involved In The Resistance Of Some Smoked Tropical Fish Samples To Insect Attack, Akinola John Akintola, Oluwatoyin Lawal Jan 2011

Biochemical Factors Involved In The Resistance Of Some Smoked Tropical Fish Samples To Insect Attack, Akinola John Akintola, Oluwatoyin Lawal

Turkish Journal of Biology

The role of mineral elements (ppm), protein and oil content (%), and saponification and free fatty acid values in contributing to resistance against insect infestation was investigated in 4 fish species: Clarias gariepinus, Parachuma obscura, Hydrocynus sp., and Malapterus electricus. Smoked fish samples were purchased from a local market in Ilorin, Kwara State, Nigeria, packaged, and maintained at 4 ^°C for 7 days. They were later brought out to acclimatise, and then observed for 4 months for possible natural insect infestation. Next, crushed samples of each species were analysed using conventional chemical analysis in order to assess the role of …


The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar Jan 2011

The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar

Turkish Journal of Biology

This study investigated the effects of diazinon, an organic phosphoric insecticide, on some developmental features of Drosophila melanogaster. The aim was to determine if any developmental inhibition occurs when diazinon is applied and if so, to determine which developmental stages are affected. Therefore, diazinon was applied to Drosophila melanogaster eggs, larvae, and adults, and its effects were observed. During the experiments 3 concentrations with values lower than LC_{50} were utilized (6 × 10^{-5}, 6 × 10^{-6}, 6 × 10^{-7} ppm). Diazinon solution was applied to Drosophila melanogaster by means of nutrition, adding it to the culture medium. The results show …


Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l Jan 2011

Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l

Turkish Journal of Biology

We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer.


The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen Jan 2011

The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen

Turkish Journal of Biology

The lethal effects resulting from incessant release of toxic surfactant-containing industrial chemicals into the Niger Delta Ecological Zone of Nigeria was studied using a laboratory toxicity test. The study employed the Organisation for Economic Development and Cooperation (OECD) 203 protocol. The test organism (Tilapia guineensis [fish]) was obtained from a brackish water environment and exposed to Neatex (industrial detergent) and Norust CR 486 (corrosion inhibitor) at concentrations of 6.25, 12.5, 25, 50, and 100 mg/L for 96 h. The ecological endpoint considered was mortality at 96 h, and the results obtained indicate that concentration and duration of exposure influenced the …


Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala Jan 2011

Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala

Turkish Journal of Biology

Detergent compatible alkaline protease production by a newly isolated Bacillus sp.Y from laundry soil was studied under shake flask conditions in growth medium comprised (g/L): peptone, 1; NaCl, 5; skim milk, 100; Na_2CO_3, 4. Different environmental process parameters such as fermentation period, initial pH, incubation temperature, and nutritional parameters such as supplementation of different starch sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Rice flour showed an almost 2.2-fold increase in protease activity followed by wheat flour, which showed a 1.85-fold increase over the basal media. However, supplementation of potato starch, corn starch, and ragi …


Possible Seasonal Variation Of The Fatty Acid Composition From Melanopsis Praemorsa (L., 1758) (Gastropoda: Prosobranchia), From Southeast Anatolia, Turkey, İhsan Eki̇n, Mehmet Başhan, Ridvan Şeşen Jan 2011

Possible Seasonal Variation Of The Fatty Acid Composition From Melanopsis Praemorsa (L., 1758) (Gastropoda: Prosobranchia), From Southeast Anatolia, Turkey, İhsan Eki̇n, Mehmet Başhan, Ridvan Şeşen

Turkish Journal of Biology

Possible seasonal variations of the fatty acid composition (neutral lipid, phospholipid, and total lipid content) of the freshwater snail Melanopsis praemorsa were investigated over the course of a year. Snail body lipids were fractionated by thin layer chromatography. Fatty acid composition of total body lipid, phospholipid, and neutral lipid of whole snail and total lipid of their algal food were analyzed by capillary gas chromatography and gas chromatography-mass spectrometry. The most abundant fatty acids were C16:0, C18:0, C16:1\omega7, C18:1\omega9, C18:2\omega6, and C18:3\omega3 acids in the lipid analyses of the snail and in its food. Odd-numbered fatty acids and C20 polyunsaturated …


Evaluation Of Antibacterial, Antifungal, Antiviral, And Antioxidant Potentials Of Some Edible Oils And Their Fatty Acid Profiles, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Bi̇lge Şener Jan 2011

Evaluation Of Antibacterial, Antifungal, Antiviral, And Antioxidant Potentials Of Some Edible Oils And Their Fatty Acid Profiles, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Bi̇lge Şener

Turkish Journal of Biology

In the current study, the oils obtained from the nuts of Corylus avellana L. (hazelnut), Arachis hypogea L. (peanut, groundnut), Pinus pinea L. (umbrella nut), and Juglans regia L. (walnut) were tested for their antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and antimicrobial activity against the standard and isolated strains of Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, and Enterococcus faecalis as well as the fungi Candida albicans and C. parapsilosis by microdilution method, in which inhibition was expressed as minimum inhibition concentrations (MICs). Moreover, antiviral activity of the oils was determined using Herpes simplex (HSV) …


An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen Jan 2011

An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen

Turkish Journal of Biology

The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was …


The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak Jan 2011

The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak

Turkish Journal of Biology

The efficacy of boric acid (BA) was examined on liver marker enzymes in aluminum (Al)-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride (5 mg/kg body weight (b.w.) AlCl3) and BA (3.25, 13, 36 and 58.5 mg/kg b.w.) alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels …


Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj Jan 2011

Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj

Turkish Journal of Biology

A total of 94 marine actinobacterial isolates were found to be associated with 4 sponges: Callyspongia diffusa, Mycale mytilorum, Tedania anhelans, and Dysidea fragilis. Among the cultures 7 strains showed antagonism against fish and shellfish pathogens. Analysis of morphological, physiological, biochemical, and protein profile characteristics suggested that the actinobacterial strains belonged to the genus Streptomyces. The initial screening of the isolates by the spot inoculation method exhibited antibacterial activity against Aeromonas hydrophila. In vitro screening of the submerged culture extracts showed positive inhibition against the fish and shellfish pathogens Aeromonas hydrophila, Serratia sp., and Vibrio spp. The screening of bioactive …


Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz Jan 2011

Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz

Turkish Journal of Biology

In this study genetic variation was determined with isozyme analysis in 8 natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems (ACP, AOX, CAT, HBDH, HXK, MDH, and SOD) were resolved using the native polyacrylamide gel electrophoresis (native-PAGE) technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus (Ne) was 1.314 ± 0.454. The mean estimated expected heterozygosity (He) …


The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais Jan 2011

The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais

Turkish Journal of Biology

The analgesic effect of the ethanolic whole plant extract of Matricaria aurea L. (Asteraceae) was studied in rats using the cold water tail flick assay and in mice using the tail immersion, tail clip, acetic-acid-induced writhing, and formalin pain tests. The results showed dose-dependent and significant (P < 0.001) increases in pain threshold at 60 min post-treatment with doses of 100, 200, and 300 mg/kg of the extract in tail flick, tail immersion, and tail clip tests. The effects of the extract were significantly (P < 0.001) lower than those produced by morphine (10 mg/kg) in the same tests. The extract (100, 200, and 300 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition in both phases of the formalin pain test, but with a less intense effect in the first phase. The results indicate that the analgesic effect of M. aurea ethanolic extract is both centrally and peripherally mediated.


Expression Of Β-(1,3-1,4)-Glucanase Gene Of Orpinomyces Sp. Gmlf18 In Escherichia Coli Ec1000 And Lactococcus Lactis Subsp. Cremoris Mg1363, Uğur Çömlekci̇oğlu, Emi̇n Özköse, İsmai̇l Akyol, Feri̇t Can Yazdiç, Mehmet Sai̇t Eki̇nci̇ Jan 2011

Expression Of Β-(1,3-1,4)-Glucanase Gene Of Orpinomyces Sp. Gmlf18 In Escherichia Coli Ec1000 And Lactococcus Lactis Subsp. Cremoris Mg1363, Uğur Çömlekci̇oğlu, Emi̇n Özköse, İsmai̇l Akyol, Feri̇t Can Yazdiç, Mehmet Sai̇t Eki̇nci̇

Turkish Journal of Biology

A gene encoding \beta-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia coli. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16. The main LicA activity was observed to be cell-associated for the licA containing transformant E. coli, and the enzyme expressed in E. coli showed the highest activity at pH 5.0-6.0 and at temperatures of 40-50 °C. The enzyme was found to be stable at 40 °C; however, 12% of LicA activity was lost at …


The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç Jan 2011

The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç

Turkish Journal of Biology

: The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be …


Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz Jan 2011

Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz

Turkish Journal of Biology

A series of studies were carried out to determine the succession and phytoplankton community of the Melen River in the Western Black Sea region of Turkey. The study was conducted at 6 stations at monthly intervals between May 2003 and April 2004. It was observed that the abundance and biovolume of phytoplankton were low in winter, though higher values were recorded in late spring and summer. Among the values noted, the spring peak was 534.38 × 10^5 ind. L^{-1} and 7384.8 mm^3 L^{-1} belonging to Peridinium sp. The most common diatom, Cyclostephanos dubius, was favoured by low discharge at stations …


Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h Jan 2011

Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h

Turkish Journal of Biology

The aim of the present study was to investigate the antibacterial activities of 25 propolis samples collected from various geographical regions of Turkey against 2 food-borne pathogens, Salmonella Enteritidis ATCC 13076 and Listeria monocytogenes ATCC 1462. The chemical compositions of ethyl alcohol extracts of the propolis (EEP) samples were determined by gas chromatography coupled to mass spectrometry. The main components of EEP samples were flavonoids, aromatic acid esters, aromatic alcohols, aromatic acids, aliphatic carboxylic acids, terpens, and aliphatic carboxylic acid esters. Antibacterial activities of the EEP samples were tested at 2 different dilutions of 1:10 and 1:100 (v/v). All EEP …


The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns Jan 2011

The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns

Turkish Journal of Biology

To evaluate possible interactions between the renin-angiotensin system (RAS) and the sympathetic nervous system (SNS) in the systemic circulation upon the intravenous administration of angiotensin II and a set of adrenergic agonists in normal Sprague-Dawley (SD) rats. Rats were treated with carvedilol, a non-selective \beta- and selective \alpha_1-adrenoceptor blocker, in a dose of (5 mg/kg) orally for 7 days, and on day 8, the animals were pentobarbitone-anaesthetized and prepared for systemic hemodynamic study. Dose-response relationships in terms of elevation in the magnitude of the mean arterial blood pressure in response to intravenous injection of noradrenaline (NA), phenylephrine (PE), methoxamine (ME), …


Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu Jan 2011

Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu

Turkish Journal of Biology

This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …


Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre Jan 2011

Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre

Turkish Journal of Biology

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …


Increased Production Of Coleoptera-Specific Delta-Endotoxin By Homologous Expression Of Cry3aa11 In Bacillus Thuringiensis Mm2, Aslihan Kurt, Gülay Özcengi̇z Jan 2011

Increased Production Of Coleoptera-Specific Delta-Endotoxin By Homologous Expression Of Cry3aa11 In Bacillus Thuringiensis Mm2, Aslihan Kurt, Gülay Özcengi̇z

Turkish Journal of Biology

The cry3Aa11 gene of Bacillus thuringiensis strain Mm2 (B. thuringiensis Mm2) was PCR-amplified and cloned into E. coli/B. thuringiensis shuttle vector pHT315. The recombinant plasmid carrying the cry3Aa11 gene was introduced into the parental B. thuringiensis Mm2. The toxin production capacities of parental and recombinant strains grown in DSM, containing either 50 mM or 200 mM inorganic phosphate (Pi), were compared by SDS-PAGE and western blot analyses. In comparison to the parental strain, recombinant B. thuringiensis Mm2 produced 4-fold more Cry3Aa11 toxin after 12 h of growth in the presence of 50 mM Pi. However, the 200 mM Pi concentration …


Phosphate Solubility And Biocontrol Activity Of Trichoderma Harzianum, Padmavathi Tallapragada, Madhumathi Gudimi Jan 2011

Phosphate Solubility And Biocontrol Activity Of Trichoderma Harzianum, Padmavathi Tallapragada, Madhumathi Gudimi

Turkish Journal of Biology

Biological control of soilborne plant pathogens is a possible alternative to the use of chemical pesticides, which are harmful to the environment. Under greenhouse and field conditions, several isolated strains of the fungus Trichoderma have been found to be effective biocontrol agents of various soilborne plant pathogenic fungi. The use of phosphate solubilizing microorganisms (PSMs) has a considerable synergistic effect on the growth and development of crop plants. In the present study, T. harzianum was isolated from 10 different agro-climatic zones of Karnataka and tested for the potential of antagonistic activity against the plant pathogen Xanthomonas and the ability of …


Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan Jan 2011

Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan

Turkish Journal of Biology

The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …


Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak Jan 2011

Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak

Turkish Journal of Biology

The chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom from southwestern Anatolia, Tricholoma anatolicum, were analyzed. Moisture, crude oil, protein, ash, total carbohydrate content, and mineral composition of the mushrooms studied, including Fe, Na, K, Zn, Cu, Ca, Cd, and Pb, were determined. The energy values of the samples were also calculated. The analyses were conducted during the 3 different growing stages of the mushrooms: mycelium, young fruiting bodies, and mature fruiting bodies. The highest values for moisture and crude oil contents were found to be in the mycelium, ash, and carbohydrate content of young fruiting bodies. …


C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz Jan 2011

C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz

Turkish Journal of Biology

In the present study, the 2n = 54 chromosomal race of blind mole rats, Nannospalax xanthodon superspecies, from Kırıkkale Province in Turkey was investigated. Conventional chromosome staining, Ag-NOR (Nucleolus Organizer Region) staining, and C-banding analysis were carried out on specimens of mole rats. The karyotype including 3 metacentric pairs (nos. 1-3), 3 submetacentric pairs (nos. 4-6), 3 subtelocentric pairs (nos. 7-9), and 17 acrocentric pairs (nos. 10-26) of autosomes (NFa = 70). C-heterochromatin regions were found in the centromeric and pericentromeric region and the short arms of some bi-armed autosomal pairs, and C-heterochromatin was localized in pericentromeric areas of a …


Cytogenetic And Biochemical Effects Of Imazethapyr In Wheat (Triticum Durum), Manijeh Hoseiny Rad, Ali Ashraf Aivazi, Shobha Jagannath Jan 2011

Cytogenetic And Biochemical Effects Of Imazethapyr In Wheat (Triticum Durum), Manijeh Hoseiny Rad, Ali Ashraf Aivazi, Shobha Jagannath

Turkish Journal of Biology

Mitotic abnormalities, chromosomal behavior, and protein content in wheat (Triticum durum) due to imazethapyr (IM) treatment were determined. Wheat seeds (variety HI-8663) were exposed to 5 IM concentrations (0.5, 0.89, 1.58, 2.81, and 5 ppm) according to the between paper method, in laboratory conditions. The protein content in the root-shoot axis and endosperm decreased significantly with the dosage increase. Cytological observations showed that the mitotic frequency in the root meristematic cells decreased parallel to the increase in concentrations. The most common types of the observed anomalies were sticky chromosomes, lagging chromosomes, scattered chromosomes, chromatin bridges, and micronuclei. The results revealed …


A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan Jan 2011

A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan

Turkish Journal of Biology

The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential …


Bioassay Studies Of 2'-O-Ethylmurrangatin Isolated From A Medicinal Plant, Murraya Paniculata, Azizuddin Shaikh, Muhammad Iqbal Choudhary Jan 2011

Bioassay Studies Of 2'-O-Ethylmurrangatin Isolated From A Medicinal Plant, Murraya Paniculata, Azizuddin Shaikh, Muhammad Iqbal Choudhary

Turkish Journal of Biology

A secondary metabolite, 2^'-O-ethylmurrangatin (1), was isolated from the leaves of a medicinal plant, Murraya paniculata. The structure of compound 1 was identified with the help of spectroscopic techniques. Compound 1 was screened for its antioxidant, lipoxygenase, prolyl endopeptidase, and respiratory burst inhibitory activities. 2^'-O-Ethylmurrangatin (1) was found to have significant activity against lipoxygenase enzyme and moderate respiratory burst inhibitory activity, but no activity against either DPPH radical scavenging assay or prolyl endopeptidase enzyme.


Anti-Inflammatory Activity Of Anthemis Aciphylla Var. Aciphylla Boiss., Si̇nem Özkaynak, Hati̇ce Efsun Kolatan, Osman Yilmaz, Bi̇jen Kivçak Jan 2011

Anti-Inflammatory Activity Of Anthemis Aciphylla Var. Aciphylla Boiss., Si̇nem Özkaynak, Hati̇ce Efsun Kolatan, Osman Yilmaz, Bi̇jen Kivçak

Turkish Journal of Biology

Ethanolic (EEAA) and sesquiterpene lactone (SEAA) extracts of the aerial parts of Anthemis aciphylla var. aciphylla Boiss. (Asteraceae) were evaluated for anti-inflammatory activity on carrageenin-induced paw edema (acute model) and cotton pellet-induced granuloma (chronic model) in rats. In carrageenin-induced paw edema, EEAA and SEAA at intraperitoneal doses of 50, 100, and 200 mg/kg dose-dependently inhibited the paw edema. In cotton pellet-induced granuloma, the oral administration of EEAA and SEAA at 50, 100, and 200 mg/kg dosages was also found to significantly inhibit granuloma tissue formation.