Digital Commons Network^™

The Effect Of Diet On The Bovine Rumen Microbial Community Structure And Composition And Its Effects On Methane Production In Growing And Finishing Cattle, Allison L. Knoell

Department of Animal Science: Dissertations, Theses, and Student Research

Methane is a potent greenhouse gas (GHG) that contributes to global warming. A natural by-product of ruminant fermentation is the production and eructation of methane. Methane is produced by a small unique group of microorganism’s called methanogens that belong to the domain Archaea. Enteric methane represents 2-12% energy loss in ruminants. It is well established that diet affects the microbial community structure and composition. Fermentative products of the mixed microbial population (bacteria, fungi, and protozoa) become the substrates for methanogens. These substrates influence which microorganisms will thrive. However, the effect of diet on the microbial community while simultaneously calculating …

Wpa News 99 (2016), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (Spring 2016), number 99

Published by the World Pheasant Association

A Retrospective Analysis Of Trends In Central Ohio Wildlife Health Using Records From A Wildlife Rehabilitation Facility, Rachel B. Dalton

Undergraduate Honors Thesis Projects

The analysis of records from wildlife rehabilitation facilities has shown great potential as a technique for monitoring health trends in local wildlife populations. We examined 45,668 records of animals admitted to a wildlife rehabilitation facility located in Ohio over a 10 year period (2005-2014). The objective was to examine how causes of admission for commonly admitted species may change over time and co-vary with seasonal patterns, with the goal of using fluctuations in wildlife admissions as a monitoring technique for population and ecosystem health. We assigned causes of admission to broad categories, such as “Collision with Non-Moving Object”, and a …

Section Abstracts: Agriculture, Forestry And Aquaculture

Virginia Journal of Science

Abstracts of the Agriculture, Forestry and Aquaculture Section for the 94th Annual Virginia Academy of Science Meeting, May 18-20, 2016, at University of Mary Washington, Fredericksburg, VA.

Is Diet Selection By Greater Sage-Grouse Influenced By Biomass Availability Or Toxins?, Jacqueline Peña, Marcella Fremgen

McNair Scholars Research Journal

Foraging herbivores must meet nutritional requirements by not only finding enough plant biomass to consume, but also finding plants with high protein content and low concentrations of potentially toxic plant secondary metabolites (PSMs). Greater sage-grouse (Centrocercus urophasianus; hereafter, sage-grouse) are sagebrush obligate herbivores that consume relatively high concentrations of PSMs. To meet their nutritional needs and avoid ingesting high amounts of PSMs, sage-grouse may select species of sagebrush for food that have lower concentrations of PSMs than a more abundant species with higher concentration of PSMs. Diet selection by sage-grouse may also be driven by chemical factors at finer scales …

Population And Morphological Changes In American Kestrels Through Space And Time, Teresa E. Ely

School of Biological Sciences: Dissertations, Theses, and Student Research

A once common raptor, the American kestrel (Falco sparverius) has experienced population declines in the last two decades throughout North America. Many hypotheses exist about the decline, including mortality from West Nile virus, rodenticide poisoning, climate change, an increase in predators, and core habitat loss or degradation, which could influence food availability. Food availability is key to raptor survival and reproduction, and changes in food availability throughout the year can have lifelong effects on size and body condition. Here we examine how morphology, specifically mass and wing chord, has changed at seven migration sites throughout North America as …

Evaluation Of Negative Energy And Mineral Balance In Periparturient Holstein-Friesian Cattle, Ameer Ahmed Abdelhameed

Open Access Dissertations

Most cows experience a state of negative energy and mineral balance during the periparturient period. This imbalance is a major problem in the dairy industry and it is more frequently seen in multiparous cattle due to their high milk production. A large amount of fat and protein is mobilized in order to address the energy deficit during the periparturient period. The ability to accurately monitor energetics and electrolytes status using accurate, rapid, and low cost point-of-care instruments will assist in optimizing animal health and productivity.

This dissertation contains three studies that evaluated point-of-care instruments for measuring blood and plasma glucose, …

Patterns Of Olfactory Neurogenesis And Immune Response In Adult Zebrafish Following Damage Of The Olfactory Bulb, Darcy M. Trimpe

Dissertations

The adult zebrafish olfactory system, with its persistent neuronal turnover and abundant regenerative capacity, is an excellent model system for studies of adult brain plasticity. Reversible deafferentation, achieved by repeated intranasal irrigation with detergent, reduces bulbar innervation and affects bulb structure and function, while cessation of treatment permits reinnervation and recovery of bulb volume. Alterations in cell genesis may account for this phenomenon. This study focuses on adult neurogenesis in the zebrafish olfactory system following reversible deafferentation and direct olfactory bulb injury.

Potential alterations in cell proliferation, differentiation, migration, and survival in the olfactory bulb following single and repeated intranasal …

Assessing Temporal Genetic Variation In A Cougar Population: Influence Of Harvest And Neighboring Populations, Rebeca L. Juarez, Michael K. Schwartz, Kristine L. Pilgrim, Daniel J. Thompson, Stephanie A. Tucker, Joshua B. Smith, Jonathan A. Jenks

Natural Resource Management Faculty Publications

The geography of the Black Hills region of South Dakota and Wyoming may limit connectivity for many species. For species with large energetic demands and large home ranges or species at low densities this can create viability concerns. Carnivores in this region, such as cougars (Puma concolor), have the additive effect of natural and human-induced mortality; this may act to decrease long-term viability. In this study we set out to explore genetic diversity among cougar populations in the Black Hills and surrounding areas. Specifically, our objectives were to first compare genetic variation and effective number of breeders of …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 SDSU Extension Annual Report.

[Page] 2 Contours Program Launched: Aims to Provide Insights & Prompt Discussions
[Page] 4 Local Momentum: Formation of State's First “Food Hub” Comes To Fruition [Page] 6 Spotlight On Soil Health: New Coalition Formed
[Page] 8 Exploring A Range Of Options: Youth Programs Cultivate Interest In Natural Resource Careers
[Page] 9 Profiles In Leadership: Our Mission Continues… Ensuring All Citizens Have Access To Education & Emerging Innovation
[Page] 10 Encouraging Animal Well-Being Workshops & Trainings Help Livestock Producers Become Better Stewards
[Page] 12 Wellness Wisdom: Variety Of Efforts Available To Encourage …

Molecular Analysis Confirming The Introduction Of Nile Crocodiles, Crocodylus Niloticus Laurenti 1768 (Crocodylidae), In Southern Florida, With An Assessment Of Potential For Establishment, Spread, And Impacts., Michael R. Rochford, Kenneth L. Krysko, Frank J. Mazzotti, Matthew W. Shirley, Mark W. Parry, Joseph A. Wasilewski, Jeffrey S. Beauchamp, Christpher R. Gillette, Edward F. Metzger Iii, Michiko A. Squires, Louis A. Somma

Papers in Herpetology

The state of Florida, USA, has more introduced herpetofauna than any other governmental region on Earth. Four species of nonnative crocodilians have been introduced to Florida (all since 1960), one of which is established. Between 2000–2014 we field-collected three nonnative crocodilians in Miami-Dade County, Florida, and one in Hendry County, Florida. We used DNA barcoding and molecular phylogenetics to determine species identification and native range origin. Also, we described diet, movement, and growth for one crocodile. Our molecular analyses illustrated that two of the crocodiles we collected are most closely related to Nile Crocodiles (Crocodylus niloticus) from South Africa, suggesting …

Diversity-Dependent Cladogenesis Throughout Western Mexico: Evolutionary Biogeography Of Rattlesnakes (Viperidae: Crotalinae: Crotalus And Sistrurus), Christopher Blair, Santiago Sánchez-Ramírez

Publications and Research

Rattlesnakes (Crotalus and Sistrurus) represent a radiation of approximately 42 species distributed throughout the New World from southern Canada to Argentina. Interest in this enigmatic group of snakes continues to accrue due, in part, to their ecomorphological diversity, contributions to global envenomations, and potential medicinal importance. Although the group has garnered substantial attention from systematists and evolutionary biologists for decades, little is still known regarding patterns of lineage diversification. In addition, few studies have statistically quantified broad-scale biogeographic patterns in rattlesnakes to ascertain how dispersal occurred throughout the New World, particularly among the different major biomes of the …

Acoustic Detection Reveals Fine-Scale Distributions Of Myotis Lucifugus, Myotis Septentrionalis, And Perimyotis Subflavus In Eastern Nebraska, Jeremy A. White, Cliff Lemen, Patricia Freeman

School of Natural Resources: Faculty Publications

ABSTRACT.—Before white-nose syndrome arrives in Nebraska, it is important to document the preexposure distributions of cave bats in the state. We examined the distributions of Myotis lucifugus (little brown myotis), Myotis septentrionalis (northern long-eared myotis), and Perimyotis subflavus (tri-colored bat) in eastern Nebraska by setting acoustic detectors for a single night at 105 sites in wooded habitats during summers of 2012 and 2014. We compared 2 methods of determining presence at each site. Results of our analyses are fine-scale distributional maps for these bats and some range extensions from published records. Results for M. septentrionalis and P. subflavus are largely …

Avifaunal Community Composition In A Tropical Forest Corridor: A Case Study From The Atherton Tableland, North Queensland, Don Jones

Independent Study Project (ISP) Collection

Bird communities in tropical forest ecosystems are highly threatened by habitat loss and fragmentation. Replanted corridors connecting isolated forest remnants are a popular method of ameliorating certain negative impacts of habitat fragmentation. Such linkages can theoretically facilitate greater dispersal, increase gene flow, and reduce the risk of local extinctions in forest birds. However, relatively few studies have examined the utilization of reforested corridors by birds, and little hard data exists to support claims that this type of resource and time intensive project is the best use of often scarce funding for conservation. This study examined the avifaunal community present in …

New Clues To A Mass Extinction: Colby Geologist Robert Gastaldo And Student Researchers Unearth Evidence That Contradicts Prevailing Models About Ancient Die-Offs, Stephen Collins

Colby Magazine

Colby geologists are rewriting deep time history, altering the script of how scientists understand the mother of all mass extinctions—the End-Permian event that occurred approximately 252 million years ago. Or to suggest that they don’t, in fact, understand it.

The Line Drawn On Pain Still Holds, Bjorn H. Merker

Animal Sentience

The many substantive criticisms raised against Key by me and by many of the other commentators will not disappear by ignoring or waving them aside with meta-discourse about anthropomorphism, just-so stories, or celestial teapots. The conceptual edifice Key inhabits and defends with such gusto may look like an impregnable fortress from the inside – and Key behaves as if it were. From the outside, however, it looks more like a ramshackle structure gaping with holes and pieced together from imperfectly understood neuroscience and often faulty literature citations.

Slavery, Welfare And The Sixth Extinction, Stephen R. Clark

Animal Sentience

Ng’s laudable concern for animal welfare would be welcome to any sensible slave-owner wishing to preserve his investment. What welfarism – for slave-owners and animal husbandmen – fails to call into question is whether we have the right to breed, hold captive and kill animals at all: If it matters, as the widely recognized slogan of ‘Five Freedoms’ suggests, that animals have the chance to live a ‘normal’ life, then more matters than keeping them ‘happy’ in subjection. Their lives – and also the lives of wild things – also deserve respect.

Intrauterine Growth-Restricted Sheep Fetuses Exhibit Smaller Hindlimb Muscle Fibers And Lower Proportions Of Insulin-Sensitive Type I Fibers Near Term, Dustin T. Yates, Caitlin N. Cadaret, Kristin A. Beede, Hannah E. Riley, Antoni R. Macko, Miranda J. Anderson, Leticia E. Camacho, Sean W. Limesand

Department of Animal Science: Faculty Publications

Intrauterine growthrestricted sheep fetuses exhibit smaller hindlimb muscle fibers and lower proportions of insulin-sensitive Type I fibers near term. Am J Physiol Regul Integr Comp Physiol 310: R1020–R1029, 2016. First published April 6, 2016; doi:10.1152/ajpregu.00528.2015.—Intrauterine growth restriction (IUGR) reduces muscle mass and insulin sensitivity in offspring. Insulin sensitivity varies among muscle fiber types, with Type I fibers being most sensitive. Differences in fibertype ratios are associated with insulin resistance in adults, and thus we hypothesized that near-term IUGR sheep fetuses exhibit reduced size and proportions of Type I fibers. Placental insufficiency-induced IUGR fetuses were 54% smaller (P < 0.05) than controls and exhibited hypoxemia and hypoglycemia, which contributed to 6.9- fold greater (P < 0.05) plasma norepinephrine and 53% lower (P < 0.05) plasma insulin concentrations. IUGR semitendinosus muscles contained less (P < 0.05) myosin heavy chain-I protein (MyHC-I) and proportionally fewer (P < 0.05) Type I and Type I/IIa fibers than controls, but MyHC-II protein concentrations, Type II fibers, and Type IIx fibers were not different. IUGR biceps femoris muscles exhibited similar albeit less dramatic differences in fiber type proportions. Type I and IIa fibers are more responsive to adrenergic and insulin regulation than Type IIx and may be more profoundly impaired by the high catecholamines and low insulin in our IUGR fetuses, leading to their proportional reduction. In both muscles, fibers of each type were uniformly smaller (P < 0.05) in IUGR fetuses than controls, which indicates that fiber hypertrophy is not dependent on type but rather on other factors such as myoblast differentiation or protein synthesis. Together, our findings show that IUGR fetal muscles develop smaller fibers and have proportionally fewer Type I fibers, which is indicative of developmental adaptations that may help explain the link between IUGR and adulthood insulin resistance

Scorpions Of Sri Lanka (Scorpiones: Buthidae, Chaerilidae, Scorpionidae) With Description Of Four New Species Of The Genera Charmus Karsch, 1879 And Reddyanus Vachon, 1972, Stat. N., František Kovařík, Graeme Lowe, Kithsiri B. Ranawana, David Hoferek, V. A. Sanjeewa Jayarathne, Jana Plíšková, František Šťáhlavský

Euscorpius

Data about all 18 known scorpion species of Sri Lanka are summarized. All previously known species were newly collected and four new species discovered during a scorpiological expedition in 2015. Information is provided about their taxonomy, distribution, ecology, and reproductive biology, fully complemented with color photos of live and preserved specimens, as well as their habitat. Subgenus Isometrus (Reddyanus) Vachon, 1972 is elevated to genus level, Reddyanus stat. n., supported by new characters of setation on leg tarsomere II and hemi-spermatophore morphology. Charmus saradieli sp. n., Reddyanus ceylonensis sp. n., R. jayarathnei …

Acropora Habitat Evaluation And Restoration Site Selection Using A Species Distribution Modeling Approach, Katherine Wirt Ames

USF Tampa Graduate Theses and Dissertations

While populations of nearly all stony coral species along the Florida reef tract have exhibited decline, the most notable decline has occurred in the once-dominant acroporid species (Acropora cervicornis, A. palmata). Both species were listed in 2006 as threatened under the Endangered Species Act. This listing, combined with their continued decline, has resulted in large-scale restoration efforts throughout Florida and the Western Caribbean. Currently, there is little to no information regarding spatial prioritization of sites for these restoration efforts. The primary objective of this dissertation was to utilize species distribution modeling, informed by existing data from the …

Analysis Of Mammalian Carboxypeptidase O Expression Patterns, Christian Bardan

Honors Theses

Carboxypeptidase 0 (CPO) is a protease that cuts acidic amino acids from the carboxy termini of substrate proteins. This study aims to describe the expression patterns of CPO in small mammals. We have sampled tissue from four different species: thirteen-lined ground squirrel, eastern chipmunk, eastern mole, and northern short-tailed shrew. Samples were analyzed via Western blot using two CPO antibodies. Immunoreactive bands likely to be CPO, with molecular weights approximately 42 kDa, were seen predominantly in kidney, liver, and intestinal tissue samples. This suggests CPO could play a specific physiological function in these organs.

A Compartmental Model Of Animal Behavior, Saharsh Dass

Honors Theses

Animal behavior is integral to fitness and arises from complex interactions between internal and external factors. An understanding of how external environmental factors drive animal behavior is important for understanding the way organisms adapt to environmental perturbations such as climate change. Glaucous-winged gulls (Larus glaucescens) at Protection Island, Strait of Juan de Fuca, Washington display a variety of behaviors on the colony during the breeding season. The most common gull behaviors are sleeping, preening, and resting. I used a system of four differential equations to predict numbers of sleeping, preening, and resting gulls on the colony as a function of …

Interactions Of A Captive Maturing Female Hamadryas Baboon, Jordyn Kessler, Janice L. Chism Ph.D.

The Winthrop McNair Research Bulletin

In the wild, hamadryas baboons (Papio hamadryas hamadryas) typically practice male philopatry, where females transfer out of natal units to avoid inbreeding (Swedell et al. 2011). However, little is known about hamadryas female transfer in captivity. In this study, we used focal animal sampling (Altmann 1974) to observe female transfer in a captive group comprised of two one-male units. The group includes a subadult female, whom we expected to transfer to the non-natal unit soon after reaching sexual maturity. We recorded the proximity between the subadult female and all other individuals, as well as affiliative and agonistic behavior …

Wild Animal Suffering And Vegan Outreach, Eze Paez

Animal Sentience

Ng’s strategic proposal seems to downplay the potential benefits of advocacy for wild animals and omit what may be the most effective strategy to reduce the harms farmed animals suffer: vegan outreach.

Declining Frequency Of Road-Killed Rabbits In Central Georgia, Anna M. Bosch, Katelyn J. Benson, Alfred J. Mead

Georgia Journal of Science

Cottontail rabbits (Sylvilagus floridanus, Sylvilagus aquaticus, Sylvilagus palustris) are a common component of the mammal fauna of the southeastern United States, yet their numbers have been in decline for at least the past 40 years. Urban sprawl, land use changes, forest maturation, and increased predation have contributed to the decline in Georgia. In the current study we explore the proposition that long-term changes in roadkill frequency track changes in wildlife populations as a whole. Using comparisons of historical data collected by Georgia Department of Natural Resources personnel and recent roadkill census data from Baldwin County, it appears that the overall …

Low-Intensity, Stocker-Based Channel Catfish Culture, Bartholomew W. Green, Carole R. Engle, Rebecca Lochmann, Harold Phillips

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Low-intensity production of Channel Catfish Ictalurus punctatus is characterized by low stocking rates, low installed aeration capacity, and no automated dissolved oxygen monitoring. Two studies conducted in nine 0.25-acre ponds quantified production characteristics of stocker Channel Catfish stocked for low-intensity food-fish production in single- or multiple-batch culture. In the multiple-batch study, 0.5-lb stockers (carryover fish) were stocked at 4,500 fish/acre and 0.044-lb fingerlings at 6,000 fish/acre. Stockers were grown to mean individual weights of 1.25, 1.75, or 2.25 lb. In the single-batch study, 0.5-lb stockers were stocked at 3,500, 4,500, and 5,500 fish/acre. All fish were fed a feed containing …

Mitigation Of Ergot Vasoconstriction By Clover Isoflavones In Goats (Capra Hircus), Glen E. Aiken, Michael D. Flythe, Isabelle A. Kagan, Huihua Ji, Lowell P. Bush

Kentucky Tobacco Research and Development Center Faculty Publications

Ergot alkaloids produced by a fungal endophyte (Epichloë coenophiala; formerly Neotyphodium coenophialum) that infects tall fescue (Lolium arundinaceum) can induce persistent constriction of the vasculature in ruminants, hindering their capability to thermo-regulate core body temperature. There is evidence that isoflavones produced by legumes can relax the vasculature, which suggests that they could relieve ergot alkaloid-induced vasoconstriction and mitigate the vulnerability to severe heat stress in ruminants that graze tall fescue. To test if isoflavones can relieve alkaloid-induced vasoconstriction, two pen experiments were conducted with rumen-fistulated goats (Capra hircus) to determine with ultrasonograpy if …

Use Of The Mitochondrial Control Region As A Potential Dna Mini-Barcoding Target For The Identification Of Canned Tuna Species, Jacquelyn K. Mitchell, Rosalee S. Hellberg

Food Science Faculty Articles and Research

In this study, a DNA mini-barcoding methodology was developed for the differentiation of species commonly found in canned tuna. Primers were designed to target a 236-base pair (bp) fragment of the mitochondrial control region (CR) and a 179-bp fragment of the first internal transcribed spacer region (ITS1). Phylogenetic analysis revealed the ability to differentiate 13 tuna species on the basis of the CR mini-barcode, except in a few cases of species introgression. Supplementary use of ITS1 allowed for differentiation of introgressed Atlantic bluefin tuna (Thunnus thynnus) and albacore tuna (Thunnus alalunga), while differentiation of introgressed Atlantic …

Analysis Of Johne's Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Background

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect …

Analysis Of Johne’S Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect Screening …