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An Investigation Of Antibacterial Effects Of Steroids, Abdullah Doğan, Sali̇h Otlu, Özgür Çelebi̇, Pinar Aksu, Ali̇ye Gülmez Sağlam, Ali̇ Nazmi̇ Can Doğan, Nesli̇han Mutlu Jan 2017

An Investigation Of Antibacterial Effects Of Steroids, Abdullah Doğan, Sali̇h Otlu, Özgür Çelebi̇, Pinar Aksu, Ali̇ye Gülmez Sağlam, Ali̇ Nazmi̇ Can Doğan, Nesli̇han Mutlu

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of steroid substances such as corticosterone, progesterone, estrone, beta-sitosterol, and stigmasterol on some bacteria were investigated. In the research, standard strains of the gram-positive bacteria Bacillus subtilis (lab isolate) and Staphylococcus aureus (ATCC 6538) and the gram-negative bacteria Escherichia coli (ATCC 25922), Pasteurella multocida (ATCC 12945), and Pseudomonas aeruginosa (ATCC 9027) were used. A modified broth dilution method was applied for determining the minimal inhibitory concentration (MIC) of the steroids. Mueller Hinton broth (Becton Dickinson, 211443) was used to prepare dilutions and as a medium. At the end of the study, the MIC of corticosterone …

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Typification Of A Fresh Goat Cheese Of Mexico By Path Models, Emmanuel J. Ramírez-Rivera, José M. Juárez-Barrientos, Jesús Rodríguez-Miranda, Pablo Díaz-Rivera, Lorena G. Ramón-Canul, José A. Herrera-Corredor, María I. Hernández-Serrano, Erasmo Herman Y Lara Jan 2017

Typification Of A Fresh Goat Cheese Of Mexico By Path Models, Emmanuel J. Ramírez-Rivera, José M. Juárez-Barrientos, Jesús Rodríguez-Miranda, Pablo Díaz-Rivera, Lorena G. Ramón-Canul, José A. Herrera-Corredor, María I. Hernández-Serrano, Erasmo Herman Y Lara

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to typify goat cheese and determine the relationship among climatic factors, production systems, milk quality, and their impact on the physicochemical, instrumental, and sensory properties and consumer preference by path models. Using the regression partial least square technique coupled to multiple factor analysis, two models were established. These models showed that the production system directly affected the physicochemical quality and indirectly affected instrumental and sensory parameters, which explained the preference and classification of the cheeses. The proposed methodology is expected to generate useful information enabling the establishment of potential fresh goat cheese produced in …

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Pathological Studies And Detection Of Different Bovine Papilloma Virus Types In Buffalocutaneous Warts, Babu Lal Jangir, Raj Bahadur Bind, Pawan Kumar, Ramesh Somvanshi Jan 2017

Pathological Studies And Detection Of Different Bovine Papilloma Virus Types In Buffalocutaneous Warts, Babu Lal Jangir, Raj Bahadur Bind, Pawan Kumar, Ramesh Somvanshi

Turkish Journal of Veterinary & Animal Sciences

Bovine papillomatosis is a common viral disease of cattle but is relatively less common in buffaloes. Bovine papilloma virus (BPV) -1 and -2 are mostly associated with cutaneous warts in buffaloes. The aim of the present work was to study the pathomorphology and association of different BPV types in buffalo cutaneous warts. A total of 10 cases were included in the present study, which revealed that growths were either single or multiple, variable in shape and size, and present at different body sites. Histopathologically, these were categorized as papilloma, fibropapilloma, and fibroma. Polymerase chain reaction (PCR) revealed the presence of …

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Proximate Analysis Of Indian Flying Fox's (Pteropus Giganteus) Natural Food, With A Note On Its Roost Variations In Urban Areas Of Lahore, Pakistan, Arshad Javid, Tayiba Gulraiz, Muhammad Ashraf, Muhammad Nadeem, Syed Makhdoom Hussain, Muhammad Altaf, Irfan Babu Jan 2017

Proximate Analysis Of Indian Flying Fox's (Pteropus Giganteus) Natural Food, With A Note On Its Roost Variations In Urban Areas Of Lahore, Pakistan, Arshad Javid, Tayiba Gulraiz, Muhammad Ashraf, Muhammad Nadeem, Syed Makhdoom Hussain, Muhammad Altaf, Irfan Babu

Turkish Journal of Zoology

Food habits, nutrient preferences, and roost variations of Indian flying fox Pteropus giganteus were investigated for a period of 1 year in two public parks in Lahore, Pakistan. Bolus and guano samples of P. giganteus were collected on a monthly basis, and seeds of 32 plant species belonging to 23 genera and 15 families were identified from the ejecta samples. Of these 32 food species, 8 were native and 24 were exotic; 13 were commercially important, while 19 species had no mercantile value. Proximate analysis of the food items revealed that fruits with comparatively higher moisture content were utilized during …

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Human Activity Mediates Reciprocal Distribution And Niche Separationof Two Sympatric Mongoose Species On The Pothwar Plateau, Pakistan, Riaz Hussain, Tariq Mahmood, Faraz Akrim, Hira Fatima, Muhammad Sajid Nadeem Jan 2017

Human Activity Mediates Reciprocal Distribution And Niche Separationof Two Sympatric Mongoose Species On The Pothwar Plateau, Pakistan, Riaz Hussain, Tariq Mahmood, Faraz Akrim, Hira Fatima, Muhammad Sajid Nadeem

Turkish Journal of Zoology

Ecologically and morphologically similar species living in sympatry are predicted to partition their resources, although the resources themselves may vary in time and space and in relation to extrinsic factors. We studied two sympatric species of carnivores that vary in their distribution along a gradient of human activity, and one species exploits a wider range of food sources. We recorded the distribution of the small Indian mongoose (Herpestes javanicus) and the gray mongoose (Herpestes edwardsii) through reconnaissance surveys within high, medium, and low human activity areas of the Pothwar Plateau from November 2011 to June 2013. We found that the …

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Macroinvertebrate Composition In The Metarhithral Zones Of The Munzur And Pülümür Rivers: A Preliminary Study, Zuhal Gülteki̇n, Rahmi̇ Aydin, Carola Winkelmann Jan 2017

Macroinvertebrate Composition In The Metarhithral Zones Of The Munzur And Pülümür Rivers: A Preliminary Study, Zuhal Gülteki̇n, Rahmi̇ Aydin, Carola Winkelmann

Turkish Journal of Zoology

The aim of this work was the determination of the macroinvertebrate community in the metarhithral zones of the Munzur and Pülümür rivers. This is the first documented research on macroinvertebrates in the metarhithral zone of the Munzur and Pülümür rivers in the region of the city of Tunceli. In total, 30 taxa of 9 orders of benthic invertebrates were recorded at the four sampling sites. Larvae of Taeniopteryx caucasica (Plecoptera), Oligoneuriella baskale, and Ecdyonurus dispar (Ephemeroptera) were recorded for the first time in the province of Tunceli.

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Distribution And Conservation Of Acomys Cilicicus (Mammalia: Rodentia) In Turkey, Ortaç Çeti̇ntaş, Ferhat Matur, Mustafa Sözen Jan 2017

Distribution And Conservation Of Acomys Cilicicus (Mammalia: Rodentia) In Turkey, Ortaç Çeti̇ntaş, Ferhat Matur, Mustafa Sözen

Turkish Journal of Zoology

Acomys cilicicus is endemic to Turkey and known from a very restricted area. The exact distribution of the species was not known up to now and the IUCN status of the species was Data Deficient (DD). To determine the exact distribution area of the species, 39 localities within the historical distribution were surveyed by using 3243 Sherman traps between 2013 and 2016. Turkish spiny mouse samples were obtained from 14 of these 39 localities and the current distribution of the species was determined. We found that the Turkish spiny mouse has two isolated populations in the area between Silifke and …

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Three New Truncatelloidean Gastropod Species From Turkey(Caenogastropoda: Littorinimorpha), Mustafa Emre Gürlek Jan 2017

Three New Truncatelloidean Gastropod Species From Turkey(Caenogastropoda: Littorinimorpha), Mustafa Emre Gürlek

Turkish Journal of Zoology

New species of Bithyniidae (Pseudobithynia adiyamanensis sp. nov.), Hydrobiidae (Pseudorientalia ceriti sp. nov.), and Bythinellidae (Bythinella yerlii sp. nov.) are described from three different locations in Turkey. Samples were collected in 2013 and 2016 and the shell and male genital tract morphology of the new species were studied. All of these new taxa were compared to congeners that live in Turkey and adjacent countries.

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Effects Of Nonaerated Circulation Water Velocity On Nutrient Release From Aquaculture Pond Sediments, Xiangju Cheng, Dantong Zhu, Xixi Wang, Deguang Yu, Jun Xie Jan 2017

Effects Of Nonaerated Circulation Water Velocity On Nutrient Release From Aquaculture Pond Sediments, Xiangju Cheng, Dantong Zhu, Xixi Wang, Deguang Yu, Jun Xie

Civil & Environmental Engineering Faculty Publications

Sustaining good water quality in aquaculture ponds is vital. Without an aerator, the dissolved oxygen in ponds comes primarily from mass transfer at the water-ambient atmosphere interface. As sediment can seriously affect water quality, this study used indoor experiments to examine the nutrient (nitrogen and phosphorus) release mechanisms and fluxes from sediment in aquaculture ponds with moving water but no aeration. The results showed that the ammonia nitrogen (NH3-N) concentration in the overlying water was inversely proportional to flow velocity and that a higher flow velocity tended to result in a lower concentration in the overlying water, a …

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Frontiers In Otolith Chemistry: Insights, Advances And Applications, B. D. Walther, K. E. Limburg, C. M. Jones, J. J. Schaffler Jan 2017

Frontiers In Otolith Chemistry: Insights, Advances And Applications, B. D. Walther, K. E. Limburg, C. M. Jones, J. J. Schaffler

OES Faculty Publications

The rapid proliferation of publications employing chemical assays of fish hard parts, and otoliths in particular, has led to many novel insights into the migration patterns, life history strategies and mixed stock dynamics of fishes across the globe (Campana et al., 2000; Elsdon et al., 2008; Walther & Limburg, 2012). These insights include uncovering diverse migratory strategies within species and populations (Kerr et al., 2009; Hogan et al., 2014; Schoen et al., 2016), quantifying rates of mixing among stocks across management boundaries (Rooker et al., 2008; Walther & Thorrold, 2010) and estimating the relative …

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Beyond Zar: The Use And Abuse Of Classification Statistics For Otolith Chemistry, C. M. Jones, M. Palmers, J. J. Schaffler Jan 2017

Beyond Zar: The Use And Abuse Of Classification Statistics For Otolith Chemistry, C. M. Jones, M. Palmers, J. J. Schaffler

OES Faculty Publications

Classification method performance was evaluated using otolith chemistry of juvenile Atlantic menhaden Brevoortia tyrannus when assumptions of data normality were met and were violated. Four methods were tested [linear discriminant function analysis (LDFA), quadratic discriminant function analysis (QDFA), random forest (RF) and artificial neural networks (ANN)] using computer simulation to determine their performance when variable-group means ranged from small to large and their performance under conditions of typical skewness to double the amount of skewness typically observed. Using the kappa index, the parametric methods performed best after applying appropriate data transformation, gaining 2% better performance with LDFA performing slightly better …

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A Population Genetic Investigation Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi) On Eglin Air Force Base, Alexander S. Wendt Jan 2017

A Population Genetic Investigation Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi) On Eglin Air Force Base, Alexander S. Wendt

College of Graduate Studies: Theses & Dissertations

The reticulated flatwoods salamander (RFS) is an endangered salamander with a unique life history. One of the largest known, best studied refuges for RFS is found on Eglin Air Force Base, and these RFS have been sampled and managed extensively since 2010. My thesis seeks to better understand RFS by using genetic techniques to address several unknowns, including: 1) determining the population structuring of RFS and the manageable units for species conservation, 2) estimating the size and status of populations, 3) understanding dispersal of RFS and factors that influence this, 4) exploring the breeding biology and recruitment patterns of RFS …

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The Biological Stress Response In Bluegill Sunfish (Lepomis Macrochirus) To Variations In Environmental Temperature And Dissolved Oxygen Content., Monique Kellman Jan 2017

The Biological Stress Response In Bluegill Sunfish (Lepomis Macrochirus) To Variations In Environmental Temperature And Dissolved Oxygen Content., Monique Kellman

Honors College Theses

With the predicted increase in global water temperature and acute hypoxic episodes, knowledge of the effects these stressors can have on local aquatic life is extremely valuable. This study thereby quantified the change in metabolic rate in Bluegill sunfish, in response to increased temperature and low dissolved oxygen concentration, by utilizing intermittent flow respirometry. Both maximum metabolic rate (MMR) and resting metabolic rate (RMR) were determined in response to variations in dissolved oxygen content, specifically > 95% O2 and 40% O2. Additionally, three temperature treatment groups were established, with temperatures of 20, 25 and 30°C in order to …

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Nocturnal Roost Tree, Roost Site And Landscape Characteristics Of Carnaby's Black-Cockatoo (Calyptorynchus Latirostris) On The Swan Coastal Plain, Candice Le Roux Jan 2017

Nocturnal Roost Tree, Roost Site And Landscape Characteristics Of Carnaby's Black-Cockatoo (Calyptorynchus Latirostris) On The Swan Coastal Plain, Candice Le Roux

Theses: Doctorates and Masters

There is limited information on communal roosting in parrot species of Western Australia and other parts of the world. Carnaby’s Black-Cockatoo is an endangered species that forms large nocturnal communal roosts, and for this reason they are an ideal model species to test the characteristics or factors that are associated with roost sites. Known roost sites distributed across the Swan Coastal Plain were identified and selected through the Great Cocky Count project. A minimum of five and maximum of ten individual trees were assessed at 11 roost sites with an overall total of 95 roost trees sampled. I determined the …

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Studying Dog Emotion Beyond Expression And Without Concern For Feeling, Peter F. Cook Jan 2017

Studying Dog Emotion Beyond Expression And Without Concern For Feeling, Peter F. Cook

Animal Sentience

Studies of dog emotion have focused on the expression of social emotion, either because this is taken to suggest human-like feeling states in dogs or because it has been the most accessible signal of dog emotional processing. I argue for an approach grounded in affective neuroscience, relying on direct measures of physiology across different contexts. This work may be particularly fertile in exploring social emotion in the dog, not because dogs necessarily share human emotional states, but because they are unique in having likely evolved to fit a human social niche.

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Shoring Up The Precautionary Bar, Jon Mallatt Jan 2017

Shoring Up The Precautionary Bar, Jon Mallatt

Animal Sentience

I offer four ways to reinforce Birch’s precautionary principle so it can be used effectively and practically in deciding which animals to cover by legislation for humane treatment: (1) add one more credible indicator of sentience to the BAR rule; (2) use phylogenetic classification, not the outdated Linnaean classification, to test which animal clades have sentience; (3) disentangle the pain of suffering from sentience; and (4) reconsider the sentient status of decapods and insects to remove potential inconsistencies in the proposed framework.

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Anecdotes Can Be Evidence Too, Heather Browning Jan 2017

Anecdotes Can Be Evidence Too, Heather Browning

Animal Sentience

Birch’s criterion for the precautionary principle imposes a high evidential standard that many cases will fail to meet. Reliable, relevant anecdotal evidence suggestive of animal sentience should also to fall within the scope of the precautionary principle. This would minimize potential suffering (as happened in the case cephalopods) while further evidence is gathered.

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Animal Pain And The Social Role Of Science, Leslie Irvine Jan 2017

Animal Pain And The Social Role Of Science, Leslie Irvine

Animal Sentience

Assuming that all animals are sentient would mean ending their use in most scientific research. This does not necessarily imply an unscientific or anti-scientific stance. Examining the social role of science reveals its considerable investment in preserving the status quo, including the continued use of animal subjects. From this perspective, the use of animal subjects is a custom that science could move beyond, rather than a methodological requirement that it must defend.

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Assessing Negative And Positive Evidence For Animal Pain, Robert W. Elwood Jan 2017

Assessing Negative And Positive Evidence For Animal Pain, Robert W. Elwood

Animal Sentience

Jonathan Birch suggests that we should take one well-conducted study that produces results consistent with the idea of pain as being sufficient to invoke the animal sentience precautionary principle. Here, I consider how to balance negative and positive results from such studies using examples from my own work. I also consider which criteria of pain might provide strong inference about pain and which may prove to be weaker.

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The Value Of Bayesian Statistics For Assessing Credible Evidence Of Animal Sentience, Anil K. Seth, Zoltan Dienes Jan 2017

The Value Of Bayesian Statistics For Assessing Credible Evidence Of Animal Sentience, Anil K. Seth, Zoltan Dienes

Animal Sentience

Determining what constitutes practically relevant, statistically significant evidence for animal sentience, under the precautionary principle, could be enhanced through Bayesian statistics. A Bayesian approach allows the incorporation of multiple evidence sources through prior probabilities, the tracking of changing evidence across time, and a principled means of adjusting evidentiary bars via Bayes factors.

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Fishes Are Gaining Academic Respect, Jonathan Balcombe Jan 2017

Fishes Are Gaining Academic Respect, Jonathan Balcombe

Animal Sentience

I respond to five commentaries on my 2016 book What a Fish Knows. The commentaries express more harmony than dissent about my interpretation of fishes as cognitive, aware individuals deserving better treatment by humankind.

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What Harmful Practices? The Material Scope Of Animal Protection Legislation, Eze Paez Jan 2017

What Harmful Practices? The Material Scope Of Animal Protection Legislation, Eze Paez

Animal Sentience

Jonathan Birch proposes a criterion for the subjective scope of animal protection legislation. He says nothing about its material scope: which harmful practices it should regulate. I argue, first, that most moral views would agree that the worst forms of animal exploitation should be legally forbidden, even if there will inevitably be disagreement about some cases of animal experimentation. I also argue that, when feasible, there should be legal provisions to help wild animals.

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Consciousness Is Not Inherent In But Emergent From Life, Jon Mallatt, Todd E. Feinberg Jan 2017

Consciousness Is Not Inherent In But Emergent From Life, Jon Mallatt, Todd E. Feinberg

Animal Sentience

Reber’s theory of the cellular basis of consciousness (CBC) is right to emphasize that we should study consciousness (sentience) in its simplest form, taking its evolution into account. However, not enough evidence is presented to support CBC’s unorthodox claim that even simple, one-celled organisms are conscious. As pointed out by other commentators, the CBC seems to be based on outdated ideas about evolution and does not acknowledge that consciousness could be an evolutionary novel feature. Such emergent features are abundant in living organisms. We review our own emergentist solution, in which consciousness evolved in the elaborating nervous systems of the …

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Use Of Insects For Fish And Poultry Compound Feed In Sub-Saharan Africa - A Systematic Review, G. Ssepuuya, V. Namulawa, D. Mbabazi, S. Mugerwa, P. Fuuna, Z. Nampijja, S. Ekesi, K. K.M. Fiaboe, D. Nakimbugwe Jan 2017

Use Of Insects For Fish And Poultry Compound Feed In Sub-Saharan Africa - A Systematic Review, G. Ssepuuya, V. Namulawa, D. Mbabazi, S. Mugerwa, P. Fuuna, Z. Nampijja, S. Ekesi, K. K.M. Fiaboe, D. Nakimbugwe

All Peer-Reviewed Publications

The cost of compound feed is a constraint in intensive fish and poultry farming, contributing 60-80% of the total production costs, 70% of which is due to fish and soy meal used as protein source. This review presents the extent to which insects have been utilised as an alternative protein source in feed in sub-Saharan Africa (SSA). A key word search of agricultural, biological and multi-disciplinary databases and academic search engines was conducted for literature on the extent of research and utilisation of insects in poultry and fish feed in SSA. There is limited published information on the practice as …

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Habitat Usage Of Tri-Colored Bats (Perimyotis Subflavus) In Western Kentucky And Tennessee Post-White Nose Syndrome, Katherine Schaefer Jan 2017

Habitat Usage Of Tri-Colored Bats (Perimyotis Subflavus) In Western Kentucky And Tennessee Post-White Nose Syndrome, Katherine Schaefer

Murray State Theses and Dissertations

The tri-colored bat (Perimyotis subflavus) has declined severely across its range since 2006 due to white nose syndrome, a fungal disease causing massive bat mortality in North America. My objective was to determine distinguishing characteristics of roost trees and habitat used by tri-colored bats so that the species’ needs can be considered in management plans. I mist-netted for tri-colored bats in western Kentucky and Tennessee during the summers of 2015 and 2016, and attached a radio transmitter to each captured adult bat. I tracked 15 bats to their day roosts and collected habitat data at 38 roost areas …

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A Risk Assessment And Phylogenetic Approach, Culum Brown Jan 2017

A Risk Assessment And Phylogenetic Approach, Culum Brown

Animal Sentience

The precautionary principal is often invoked when talking about the evidence of sentience in animals, largely because we can never be certain what any animal is thinking or feeling. Birch (2017) offers a preliminary framework for the use of the precautionary principal for animal sentience combining an epistemic rule with a decision rule. I extend this framework by adding an evolutionary phylogentic approach which spreads the burden of proof across broad taxonomic groups and a risk assessment component which magnifies the likely impact by the number of animals involved.

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Assessment Of A Channel Catfish Population In A Large Open River System, A. J. Blank, M. J. Hamel, Jonathan J. Spurgeon, Mark A. Pegg Jan 2017

Assessment Of A Channel Catfish Population In A Large Open River System, A. J. Blank, M. J. Hamel, Jonathan J. Spurgeon, Mark A. Pegg

School of Natural Resources: Faculty Publications

Estimates of dynamic rate functions for riverine channel catfish, Ictalurus punctatus (Rafinesque), populations are limited. The open nature and inherent difficulty in sampling riverine environments and the propensity for dispersal of channel catfish impede estimation of population variables. However, contemporary population models (i.e. robust design models) can incorporate the open nature of these systems. The purpose of this study was to determine channel catfish population abundance, survival and size structure and to characterize growth in the lower Platte River, Nebraska, USA. Annual survival estimates of adult channel catfish were 13%–49%, and channel catfish abundance estimates ranged from 8,281 to 24,261 …

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Population Characteristics And Trophic Interactions Between Pacific Mole Crabs And Redtail Surfperch On Northern California Sandy Beaches, Michelle Lynn Succow Jan 2017

Population Characteristics And Trophic Interactions Between Pacific Mole Crabs And Redtail Surfperch On Northern California Sandy Beaches, Michelle Lynn Succow

Cal Poly Humboldt theses and projects

A network of marine protected areas (MPAs) was established to protect northern California coastal habitats in December 2012. Populations of two indicator species, Amphistichus rhodoterus (redtail surfperch) and Emerita analoga (pacific mole crabs) were characterized within MPAs and reference sites to provide baseline ecological information for this region. Data were gathered using a series of simple field surveys (hook and line fishing surveys for A. rhodoterus along with core and transect surveys for E. analoga). Relative abundance, lengths, and sex ratios of A. rhodoterus did not differ between MPAs and their respective reference sites, whereas condition (overall health) increased …

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Mobbing Behavior In Wild Steller's Jay (Cyanocitta Stelleri), Kelly Anne Commons Jan 2017

Mobbing Behavior In Wild Steller's Jay (Cyanocitta Stelleri), Kelly Anne Commons

Cal Poly Humboldt theses and projects

Mobbing is a widespread anti-predator behavior with multifaceted functions. Mobbing behavior has been found to differ with respect to many individual, group, and encounter level factors. To better understand the factors that influence mobbing behavior in wild Steller’s jays (Cyanocitta stelleri), I induced mobbing behavior using 3 predator mounts: a great horned owl (Bubo virginianus), common raven (Corvus corax), and sharp-shinned hawk (Accipiter cooperii). I observed 90 responses to mock predators by 33 color-marked individuals and found that jays varied in their attendance at mobbing trials, their alarm calling behavior, and in …

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Abundance, Growth, And Predation By Non-Native Brown Trout In The Trinity River, Ca, Justin S. Alvarez Jan 2017

Abundance, Growth, And Predation By Non-Native Brown Trout In The Trinity River, Ca, Justin S. Alvarez

Cal Poly Humboldt theses and projects

Brown Trout were introduced to the Trinity River in Northern California in the 1890’s. Since 1932, Brown Trout have sustained their population without additional stocking. Over the last 15 years, fisheries managers have been concerned that predation by piscivorous Brown Trout may impede efforts to restore native salmonids, in particular endangered Coho Salmon. I investigated predation by Brown Trout on native fish in the 64 km of the main stem Trinity River below Lewiston Dam. Using a bioenergetics approach parameterized with field measurements of Brown Trout abundance and growth, I estimated the amount of energy needed to sustain the 2015 …

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