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Effect Of Morinda Citrifolia (Noni)-Enriched Diet On Hepatic Heat Shock Protein And Lipid Metabolism-Related Genes In Heat Stressed Broiler Chickens, Joshua Flees

Graduate Theses and Dissertations

Heat stress (HS) has been reported to alter fat deposition in broilers, however the underlying molecular mechanisms are not well-defined; therefore, the objectives of the current study were to: (1) determine the effects of acute (2 h) and chronic (3 wk) HS on the expression of key molecular signatures involved in hepatic lipogenic and lipolytic programs; and (2) assess if diet supplementation with dried Noni medicinal plant (0.2% of the diet) modulates these effects. Broilers (n=480 males, 1 d) were randomly assigned to 12 environmental chambers, subjected to two environmental conditions (HS at 35°C vs. thermoneutral condition [TN] at 24°C) …

Effect Of Yeast Supplementation During Various Stages Of Beef Production, Elizabeth Palmer

Graduate Theses and Dissertations

Supplemental dietary yeast products are beneficial during times of stress. Calves experience increased levels of stress during birth, weaning, and the post-weaning receiving period. Therefore, 3 experiments were conducted to determine how yeast supplementation affects growth performance and health during this time. In experiment 1, 2 truckloads of highly stressed calves (n = 175; initial body weight [BW] = 226 ± 24.5 kg) were assigned randomly to 1 of 3 treatments; unsupplemented control, or supplementation with 2 commercial yeast products (Y1 and Y2). Average daily gain (ADG) was not different (P = 0.99) during the 28 d receiving period; nor …

Using Systematic Conservation Planning To Establish Management Priorities For Freshwater Salmon Conservation, Matanuska-Susitna Basin, Ak, Usa, Andrew W. Witt, Edd Hammill

Ecology Center Publications

1- The Alaskan Matanuska-Susitna Basin (MSB) provides habitat for all five Pacific salmon species, and their large seasonal spawning runs are important both ecologically and economically. However, the encroachment of human development through urbanization and extractive industries poses a serious risk to salmon habitat in the MSB.

2- Using systematic conservation planning techniques, different methods of incorporating anthropogenic risks were assessed to determine how to cost-effectively conserve salmon habitat in the area.

3- The consequences of four distinct conservation scenarios were quantified: no consideration of either urbanization or extractive industries (‘Risk ignored’ scenario); accounting for the risk of urbanization, and …

Towards Sustainable Aquafeeds: Evaluating Substitution Of Fishmeal With Lipid-Extracted Microalgal Co-Product (Nannochloropsis Oculata) In Diets Of Juvenile Nile Tilapia (Oreochromis Niloticus), Pallab K. Sarker, Anne R. Kapuscinski, Ashley Y. Bae, Emily Donaldson, Devin S. Fitzgerald, Oliver F. Edelson

Dartmouth Scholarship

Microalgae companies increasingly seek markets for defatted biomass that is left over after extracting omega-3 rich oil for human nutraceuticals and crude oil for fuels. Such a protein-rich co-product is a promising alternative to unsustainably sourced fishmeal in aquaculture diets. We report the first evaluation of co-product of the marine microalga Nannochloropsis oculata(N. oculata co-product) for replacing fishmeal in diets of Nile tilapia, a globally important aquaculture species. We conducted a nutrient digestibility experiment with N. oculata dried whole cells and N. oculata co-product, followed by an 84-day nutritional feeding experiment with N. oculata co-product. N. oculata co-product, more nutrient-dense …

Unique Osmoregulatory Morphology In Primitive Sharks: An Intermediate State Between Holocephalan And Derived Shark Secretory Morphology, Matthew Eric Larsen

Electronic Theses and Dissertations

Discovery of an unusual rectal gland in the Atlantic sixgill shark, Hexanchus vitulus, led to examination of rectal glands in 29 species of epipelagic and deep-sea sharks. Eight of 14 deep-sea species of sharks had digitiform glands that were previously assumed to be characteristic of elasmobranchs (N=281; mean width-length ratio ± SD = 0.18 ± 0.07). Hematoxylin-and-eosin stained sections from deep-sea sharks were similar to those from shallow water sharks. Glands from the family Somniosidae were kidney bean-shaped (N = 3; mean width-length ratio ± SD = 0.46 ± 0.05); whereas those from the families Echinorhinidae and Hexanchidae appeared lobulate …

Molecular Adaptations For Sensing And Securing Prey And Insight Into Amniote Genome Diversity From The Garter Snake Genome, Blair W. Perry, Daren C. Card, Joel W. Mcglothlin, Giulia I.M. Pasquesi, Richard H. Adams, Drew R. Schield, Nicole R. Hales, Andrew B. Corbin, Jeffery P. Demuth, Federico G. Hoffmann, Michael W. Vandewege, Ryan K. Schott, Nihar Bhattacharyya, Belinda S.W. Chang, Nicholas R. Casewell, Gareth Whiteley, Jacobo Reyes-Velasco, Stephen P. Mackessy, Tony Gamble, Kenneth B. Storey, Kyle K. Biggar, Courtney N. Passow, Chih-Horng Kuo, Suzanne E. Mcgaugh, Anne M. Bronikowski, A.P. Jason De Koning, Scott V. Edwards, Michael E. Pfrender, Patrick Minx, Edmund D. Brodie Jr., Edmund D. Brodie Jr., Wesley C. Warren, Todd A. Castoe

Biology Faculty Publications

Colubridae represents the most phenotypically diverse and speciose family of snakes, yet no well-assembled and annotated genome exists for this lineage. Here, we report and analyze the genome of the garter snake, Thamnophis sirtalis, a colubrid snake that is an important model species for research in evolutionary biology, physiology, genomics, behavior, and the evolution of toxin resistance. Using the garter snake genome, we show how snakes have evolved numerous adaptations for sensing and securing prey, and identify features of snake genome structure that provide insight into the evolution of amniote genomes. Analyses of the garter snake and other squamate reptile …

Horse Y Chromosome Assembly Displays Unique Evolutionary Features And Putative Stallion Fertility Genes, Jan E. Janečka, Brian W. Davis, Sharmila Ghosh, Nandina Paria, Pranab J. Das, Ludovic Orlando, Mikkel Schubert, Martin K. Nielsen, Tom A. E. Stout, Wesley Brashear, Gang Li, Charles D. Johnson, Richard P. Metz, Al Muatasim Al Zadjali, Charles C. Love, Dickson D. Varner, Daniel W. Bellott, William J. Murphy, Bhanu P. Chowdhary, Terje Raudsepp

Veterinary Science Faculty Publications

Dynamic evolutionary processes and complex structure make the Y chromosome among the most diverse and least understood regions in mammalian genomes. Here, we present an annotated assembly of the male specific region of the horse Y chromosome (eMSY), representing the first comprehensive Y assembly in odd-toed ungulates. The eMSY comprises single-copy, equine specific multi-copy, PAR transposed, and novel ampliconic sequence classes. The eMSY gene density approaches that of autosomes with the highest number of retained X–Y gametologs recorded in eutherians, in addition to novel Y-born and transposed genes. Horse, donkey and mule testis RNAseq reveals several candidate genes for stallion …

The Usefulness Of Gps Telemetry To Study Wolf Circadian And Social Activity, Samuel B. Merrill, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

This study describes circadian and social movement patterns of 9 wolves and illustrates capabilities and limitations of Global Positioning System (GPS) telemetry for analysis of animal activity patterns. Wolves were studied at the Camp Ripley National Guard Training Site in Little Falls, Minnesota, and were captured via helicopter net-gunning. All study wolves showed nocturnal movement patterns regardless of time of year. One wolf's movement pattern switched to diurnal when he conducted an extraterritorial foray from his natal territory. All data sets with GPS intervals <1 hour (n=4) showed crepuscular movement peaks. We identified patterns of den visitation and attendance, estimated minimum distances traveled and minimum rates of movement, and observed that GPS location intervals may affect perceived rates of wolf travel. Global Positioning System telemetry was useful in determining when pack members were traveling together or apart and how long a breeding female wolf spent near her pups (e.g., 0-month-old pups were left unattended by their mother for as long as 17 days).

Modeling And Mapping Addiction In The Zebrafish, Danio Rerio, Bradley Serpa

Master of Science in Integrative Biology Theses

Driven by the communication of dopamine, the vertebrate reward system has been evolutionarily conserved to maintain survival and optimize fitness. The neural circuits governing this system integrate sensory stimuli to produce appropriate, self-preserving responses that underlie experience-based learning. In the most primitive vertebrates, dopamine release in neuronal circuits drives homeostatic behaviors, such as seeking nutrients, finding a mate, or avoiding danger. From agnathans to mammals, dopaminergic synthesis and signaling genes and molecules, along with neuronal pathways and reward system-based behaviors, remain highly conserved. Dopamine signaling proteins include two classes of metabotropic G-Protein Receptor Coupled Dopamine Receptors, D1-like (DRD1) and D2-like …

Parasitic Indicators Of Foraging Strategies In Wading Birds, Sarah Gumbleton

HCNSO Student Theses and Dissertations

Feeding ecology and trophic interactions of six species of wading birds were explored through a combined analysis of stable isotope profiles and endoparasite communities. Stable isotopes broadly characterize the feeding preferences and geographic information, while parasite communities reflect long-term trends in feeding ecology. Deceased birds were obtained from four South Florida wildlife rehabilitation organizations. Of the 81 birds dissected, 73 contained parasites. Parasites were predominately found within the gastrointestinal tract. Host and range extensions were noted for several parasite taxa. Bird host species had a significant effect on the parasite community (P=0.001) while wildlife center location and maturity status did …

Goats Prefer Positive Human Emotional Facial Expressions, Christian Nawroth, Natalia Albuquerque, Carine Savalli, Marie-Sophie Single, Alan G. Mcelligott

Social Cognition Collection

Domestication has shaped the physiology and the behaviour of animals to better adapt to human environments. Therefore, human facial expressions may be highly informative for animals domesticated for working closely with people, such as dogs and horses. However, it is not known whether other animals, and particularly those domesticated primarily for production, such as goats, are capable of perceiving human emotional cues. In this study, we investigated whether goats can distinguish human facial expressions when simultaneously shown two images of an unfamiliar human with different emotional valences (positive/ happy or negative/angry). Both images were vertically attached to a wall on …

Nanostructured Fibers As A Versatile Photonic Platform: Radiative Cooling And Waveguiding Through Transverse Anderson Localization, Norman Nan Shi, Cheng-Chia Tsai, Michael J. Carter, Jyotirmoy Mandal, Adam C. Overvig, Matthew Y. Sfeir, Ming Lu, Catherine L. Craig, Gary D. Bernard, Yuan Yang, Nanfang Yu

Publications and Research

Broadband high reflectance in nature is often the result of randomly, three-dimensionally structured materials. This study explores unique optical properties associated with one-dimensional nanostructures discovered in silk cocoon fibers of the comet moth, Argema mittrei. The fibers are populated with a high density of air voids randomly distributed across the fiber cross-section but are invariant along the fiber. These filamentary air voids strongly scatter light in the solar spectrum. A single silk fiber measuring ~50 μm thick can reflect 66% of incoming solar radiation, and this, together with the fibers' high emissivity of 0.88 in the mid-infrared range, allows …

Assessment Of Northern Bobwhite Survival And Fitness In The West Gulf Coastal Plain Ecoregion, Jacob Doggett, Alexandra Locher

Open Access Publishing Support Funded Articles

In the West Gulf Coastal Plains (WGCP) northern bobwhite (Colinus virginianus) are declining faster than range-wide averages and such declines have been linked to the consequences of land management. Management for the endangered red-cockaded woodpecker (Picoides borealis) has benefitted northern bobwhite by restoring mature pine-grassland ecosystems in some areas of the region. However, at Felsenthal National Wildlife Refuge, Crossett, Arkansas, USA, the bobwhite population was not increasing despite the availability of seemingly suitable habitat from management for the endangered species. To understand factors that may be affecting bobwhite survival on Felsenthal National Wildlife Refuge we conducted …

Development Of On-Shore Behavior Among Polar Bears (Ursus Maritimus) In The Southern Beaufort Sea: Inherited Or Learned?, Kate M. Lillie, Eric M. Gese, Todd C. Atwood, Sarah A. Sonsthagen

Wildland Resources Faculty Publications

Polar bears (Ursus maritimus) are experiencing rapid and substantial changes to their environment due to global climate change. Polar bears of the southern Beaufort Sea (SB) have historically spent most of the year on the sea ice. However, recent reports from Alaska indicate that the proportion of the SB subpopulation observed on-shore during late summer and early fall has increased. Our objective was to investigate whether this on-shore behavior has developed through genetic inheritance, asocial learning, or through social learning. From 2010 to 2013, genetic data were collected from SB polar bears in the fall via hair snags …

Combined Use Of Edna Metabarcoding And Video Surveillance For The Assessment Of Fish Biodiversity, Michael Stat, Jeffrey John, Joseph D. Dibattista, Stephen J. Newman, Michael Bunce, Euan S. Harvey

Fisheries Research Articles

Monitoring communities of fish is important for the management and sustainability of fisheries and marine ecosystems. Baited remote underwater video systems (BRUVs) are among the most effective nondestructive techniques for sampling bony fishes and elasmobranchs (sharks, rays, and skates). However, BRUVs sample visually conspicuous biota; hence, some taxa are undersampled or not recorded at all. We compared the diversity of fishes characterized using BRUVs with diversity detected via environmental DNA (eDNA) metabarcoding. We sampled seawater and captured BRUVs imagery at 48 locales that included reef and seagrass beds inside and outside a marine reserve (Jurien Bay in Western Australia). Eighty-two …

A New Scorpion Species From Kenya, Gint Childsi Sp. N. (Scorpiones: Buthidae), Frantíšek Kovařík

Euscorpius

Gint childsi sp. n. from Kenya is described and compared with other species of the genus. Additional information is provided on the taxonomy and distribution of the genus Gint, fully complemented with color photos of preserved specimens of both sexes of the new species, as well as of their habitat.

Two New Hottentotta Species From Iran, With A Review Of Hottentotta Saulcyi (Scorpiones: Buthidae), Frantíšek Kovařík, Ersen Aydin Yağmur, Mohammed Moradi

Euscorpius

Two new buthid species from Iran, Hottentotta navidpouri sp. n. (Hormozgan Province) and H. sistanensis sp. n. (Sistan and Baluchestan Province) are described, compared with H. saulcyi (Simon, 1880), and fully illustrated with color photos. The two new species differ from H. saulcyi mainly in shape of chela, which is strongly elongated in both new species.

A New Species Of Microtityus From The British Virgin Islands, West Indies, And New Localities For Other Scorpions (Scorpiones: Buthidae, Scorpionidae), Luis F. De Armas

Euscorpius

Microtityus (Parvabsonus) eustatia sp. n. is herein described from the British Virgin Islands (West Indies): Eustatia Island (type locality), Virgin Gorda Island, and Camanoe Island, based on seven specimens (three males and four females). The new species closely resembles M. waeringi Francke & Sissom, 1980 from St. John Island and St. Thomas Island, U.S. Virgin Islands, differing mainly by the fixed finger of pedipalp having ten rows of denticles (nine in M. waeringi) and more attenuated metasoma. Also, new localities are recorded for Heteronebo yntemai Francke & Sissom, 1980 (Scorpionidae) and Centruroides griseus (C. L. Koch, 1844) …

Nine-Banded Armadillo (Dasypus Novemcinctus) In Spartanburg County, South Carolina, Jonathan J. Storm

Journal of the South Carolina Academy of Science

The distribution range of the nine-banded armadillo (Dasypus novemcinctus) has been expanding northward across the eastern and central United States over the past several decades. It is thought that armadillos first reached South Carolina in the mid-1980s in the southwestern portion of the state. Armadillo sightings are rare in the Upstate region of South Carolina. Here, we report of an armadillo photographed in southern Spartanburg County on 30 May, 2017. This individual was spotted in mixed deciduous forest along the Tyger River. Our data add further support to the notion that armadillos have expanded their breeding range into …

Follow The Rain? Environmental Drivers Of Tyrannus Migration Across The New World, Maggie P. Macpherson, Alex E. Jahn, Michael T. Murphy, Daniel H. Kim, Victor R. Cueto, Diego T. Tuero, Elliot D. Hill

Biology Faculty Publications and Presentations

Predictable seasonal changes in resources are thought to drive the timing of annual animal migrations; however, we currently understand little about which environmental cues or resources are tracked by different migratory bird species across the planet. Understanding which environmental cues or resources birds track in multiple migratory systems is a prerequisite to developing generalizable conservation plans for migratory birds in a changing global environment. Within the New World, climatic differences experienced by Nearctic–Neotropical migratory (NNM; i.e. breed in North America and spend the nonbreeding period in the Neotropics) and Neotropical austral migratory (NAM; i.e. breed and spend the nonbreeding period …

Conservation Of Freshwater Live-Bearing Fishes: Development Of Germplasm Repositories For Goodeids, Yue Liu

LSU Doctoral Dissertations

Fishes of the family Goodeidae are considered to be among the most imperiled taxa in the world. The goal of this project was to provide a foundation for development of sperm repositories for goodeids and to use them as a model to assist conservation programs for imperiled freshwater live-bearing fishes. Development of such repositories is challenging because of unusual reproductive characteristics of live-bearing fishes, such as sperm bundles, internal fertilization, and the bearing of live young. Standardized methods were established to evaluate quality-related attributes of sperm bundles. The different features between activation of free and bundled sperm were investigated and …

The Birds Of Nebraska, Paul Johnsgard

Zea E-Books Collection

This annotated list of the birds of Nebraska grew gradually out of research associated with my writing of the Birds of the Great Plains: Breeding Species and Their Distribution (Johnsgard, 1979a). It expands and updates an earlier version that was published in 2013 by the University of Nebraska–Lincoln Libraries DigitalCommons’ Zea Books (Johnsgard, 2013a). It has been updated and modified in its current revision to conform with the most recent (2017) major revision of the American Ornithologists’ Society’s Checklist of North American Birds (Chesser et al., 2017). It has also been modified in its current revision to conform very closely …

A Unique Small-Scale Microclimatic Gradient In A Temperate Karst Harbours Exceptionally High Diversity Of Soil Collembola, Natália Raschmanová, Dana Miklisová, Ľubomír Kováč

International Journal of Speleology

The collapse doline of the Silická ľadnica Ice Cave, 351 m² in area, is a unique phenomenon, a steep microclimate gradient in a low-altitude temperate karst in the Western Carpathian Mts, Slovakia, with a remarkable temperature decrease from the edge of karst plateau towards the doline bottom, which harbours perennial ice deposits. Collembola communities were studied in detail at seven sites along the 117.5 m long gradient slope during 2005–2007. An exceptionally high species richness of soil Collembola was observed, 129 species, which is about 91% of the total species richness generated by Chao1/ACE estimator. Species richness positively correlated …

Characterizing Cytoplasmic Maturation And Improving Competence Of Bovine In Vitro Matured Oocytes, Brittany Alanna Foster

LSU Doctoral Dissertations

As the necessity to improve in vitro embryo production efficiency is ever increasing, so too is the prerequisite obligation to improve oocyte competence. The ability of an oocyte to successfully produce a viable embryo requires that it both completes meiotic maturation and synchronously achieves cytoplasmic competence; an encompassing term that refers to the oocytes correct accumulation and storage of mRNAs, proteins and factors as well as the development and reorganization of organelles and the cytoskeleton. The current research was designed to analyze those methods that go into oocyte collection and pre-maturation handling to determine if there are ways to ensure …

Age-Specific Estimates Indicate Potential Deleterious Capture Effects And Low Survival Of Stocked Juvenile Colorado Pikeminnow (Ptychocheilus Lucius), Scott R. Clark, Mary M. Conner, Scott L. Durst, Nathan R. Franssen

Wildland Resources Faculty Publications

Hatcheries and stocking programs have become necessary to repatriate or augment populations of imperiled fishes worldwide. Over nearly two decades, millions of endangered juvenile Colorado Pikeminnow Ptychocheilus lucius have been stocked into the San Juan River (Colorado, New Mexico, and Utah); however, recruitment of these individuals to adult life stages (age ≥6) remains low. Using a mark–recapture data set collected from annual riverwide electrofishing efforts between 2003 and 2016, we investigated apparent survival and capture probabilities of stocked Colorado Pikeminnow to identify age‐specific bottlenecks contributing to this lack of recruitment. With relatively high capture rates, which averaged between 0.34 and …

Biodiversity And Conservation Study Of Chambers Island, Wisconsin - 2017 Annual Report, Mackenzie Hennes, Mikaela Nowak, Adam L. Brandt

Student Creative and Scholarly Works

Long term biological studies are rare but incredibly valuable for examining natural phenomena. Data collected over many years or decades allows for analyses of trends that would never be apparent in a single season. The research presented here is for the completion of the first field season in a long term study analyzing biodiversity trends on Chambers Island. Using visual searches, live trapping, and trail cameras, the biodiversity and abundance of species was examined. Special emphasis was placed on small mammals, though other species were observed and documented. The goals of this project were to (1) provide preliminary diversity and …

Australian Herring And West Australian Salmon Scientific Workshop Report, October 2017, Brent S. Wise, Brett Molony

Fisheries Research Reports

A scientific workshop was held on 25th – 29th September 2017 to review available data for Australian herring (Arripis georgianus) and determine current stock status using the Department of Primary Industries and Regional Development’s (DPIRD) Risk-based Weight of Evidence Approach (Appendix 1). Recommendations for future monitoring and assessments required to reduce uncertainties and establish sustainable catch levels were also made.

Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie

Masters Theses & Specialist Projects

While substrate-borne vibrations are utilized by different reptile species, true conspecific communication via biotremors has not yet been demonstrated in reptiles. This study follows a preliminary report that the veiled chameleon (Chamaeleo calyptratus) could produce biotremors in communicative contexts. I tested chameleon behavioral sensitivity to vibrations by placing them on a dowel attached to a shaker emitting vibrations of 25, 50, 150, 300, and 600 Hz and then measured their changes in velocity before and after the stimulus. I then paired chameleons in various social contexts [anthropogenic disturbance (human disruption of animal); dominance (malemale; female-female C. calyptratus); courtship (male-female C. …

An Evaluation Of Deterrent Methods Utilized To Prevent Crop Raiding By African Elephants (Loxodonta Africana) In The Kasigau Wildlife Corridor, Kenya, Rebecca Lynn Von Hagen

Masters Theses & Specialist Projects

Escalating human elephant conflict (HEC) continues to be a contributing factor

towards elephant decline, and crop raiding is the most common form of negative

human-elephant interactions. For communities that cannot reverse or prevent crop

raiding, it is necessary to contain HEC events through deterrent measures. Few

deterrent measures exist that combine practicality and affordability while also

preventing habituation by elephants. This project focused on comparing the efficacy of

deterrent methods to assess which was the most successful at preventing elephants

from entering crops in the farming community of Sasenyi, Kenya. In this paired-control

study, four deterrent methods were evaluated: acacia …

The Ecology Of The Usambara Double-Collared Sunbird (Cinnyris Usambaricus), Sarah Lueder

Independent Study Project (ISP) Collection

The ecology of the endemic Usambara Double-collared Sunbird (Cinnyris usambaricus) was investigated in the surrounding areas of and within Mazumbai Forest Reserve in the Lushoto District of Tanzania. As there is little known concerning the ecology of the species, this study sought to illuminate multiple aspects of their behavior and interactions. Over fourteen days (November 2nd-November 21st), the abundance of the species, their nesting behavior, their habitat preferences, their vocalizations, their diet, and their aggressive behaviors were observed and then analyzed. It was determined the species’ activity likely peaks in the early morning, their nesting is similar to other species …