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The Physico-Chemical Environment And Aquatic Biodiversity Of Head Marala Wetland During 2000-2001, Zahid Bhatti, Muhammad Asif Gondal, Andleeb Batool Apr 2019

The Physico-Chemical Environment And Aquatic Biodiversity Of Head Marala Wetland During 2000-2001, Zahid Bhatti, Muhammad Asif Gondal, Andleeb Batool

Journal of Bioresource Management

An ecological study on Head Marala Wetland was undertaken. The study will help in providing sound basis for the future conservation activities and its management, on sounder footing. The Marala wetlands in Bajwat area included river Jammu Tawi, river Chanab and river Manawar Tawi were studied for a period of one year, i.e. from October, 2000 to September, 2001. In this study, 36 Phytoplanktons belonging to six families were observed. The other aquatic fauna observed included 14 species of fish, 2 turtles, one leech, one prawn, one shrimp and one bullfrog. The results showed that physico-chemical parameters had an impact …


The Piping Plover Problem: A Review Of Management Issues For A Threatened Shorebird, Andrew Lydeard, Gerry Harris Apr 2019

The Piping Plover Problem: A Review Of Management Issues For A Threatened Shorebird, Andrew Lydeard, Gerry Harris

Scholars Week

Andrew Lydeard and Gerry Harris

The Piping Plover (Charadrius melodus) was federally listed in 1986. Since listing, Piping Plovers have been a focus of conservation and management efforts, particularly on their breeding grounds in the Northern Great Plains, Great Lakes, and northern Atlantic Coast. Despite management efforts that have resulted in range-wide population growth of the Piping Plover, growth in individual populations is often slow and reasons for this are poorly understood. A bias towards understanding drivers of declines on breeding sites compared to wintering and migratory stopover sites may be an underlying cause of this lack of …


Identifying Roadkill Hotspots Using A Running Average, Kori A. Ogletree, Alfred J. Mead, Evan R. Boitet Apr 2019

Identifying Roadkill Hotspots Using A Running Average, Kori A. Ogletree, Alfred J. Mead, Evan R. Boitet

Georgia Journal of Science

The identification of roadkill hotspots is necessary prior to the consideration of wildlife road mortality mitigation measures. In a previous study, 178 roadkill specimens were tallied via a driving survey along 21.4 km (13.3 mi) on three connected roadways in Baldwin County, Georgia. Roadkill locations were recorded to the nearest 0.16 km (0.1 mi) using the vehicle odometer. In the current study, location data were used to generate three graphical displays of roadkill distribution: 1) a linear graph of roadkills per 0.16 km (0.1 mi) bin; 2) a linear graph of roadkills per 0.8 km (0.5 mi) bin; and 3) …


Preventing Infectious Hematopoietic Necrosis In Salmonid Fish Along The Columbia River Basin, Whitney L. Wright Apr 2019

Preventing Infectious Hematopoietic Necrosis In Salmonid Fish Along The Columbia River Basin, Whitney L. Wright

OHSU-PSU School of Public Health Annual Conference

Infectious hematopoietic necrosis virus (IHNV) is a member of the Rhabdoviridae family and causes infectious hematopoietic necrosis (IHN) disease in many salmonid species during the juvenile or “fry” stage of life. IHNV is endemic to Western North America and occurs in the Columbia River Basin, where Steelhead and Chinook salmon are the most abundant IHNV-vulnerable species. IHNV can cause an epidemic in wild or farmed stocks, killing 90-95 percent of the fish it infects. Transmission is currently understood to occur by direct exposure through the gills, and the virus is shed typically from asymptomatic or clinically ill carrier adults at …


Density Of Hemigrapsus Sanguineus And Carcinus Maenas In Kettle Cove, Cape Elizabeth, Maine, Valerie Huston, Teresa Martel, Zimzim Mohamed Apr 2019

Density Of Hemigrapsus Sanguineus And Carcinus Maenas In Kettle Cove, Cape Elizabeth, Maine, Valerie Huston, Teresa Martel, Zimzim Mohamed

Thinking Matters Symposium Archive

Our focus for this project is to determine how the population densities of Hemigrapsus sanguineus, Asian shore crab and Carcinus maenas, European green crabs have shifted over a brief period of time. We are comparing data collected on September 17, 2018, to data collected on October 22, 2018. The location where the two sets of data were collected from is Kettle Cove, Cape Elizabeth, Maine. The GPS coordinance was 43°.33.55N70°.13.40N. We discuss what affects the influences of environmental factors such as temperature, salinity, high intertidal versus low intertidal, and percent ascophyllum cover affect have on the population densities of these …


The Androscoggin Bioblitz: A Citizen Science Biological Survey In New Auburn, Maine, Sam H. Matey Apr 2019

The Androscoggin Bioblitz: A Citizen Science Biological Survey In New Auburn, Maine, Sam H. Matey

Thinking Matters Symposium Archive

After several weeks of planning, the Androscoggin Citizen Biological Survey, or “BioBlitz,” took place on August 11th, 2018 in New Auburn, near the confluence of the Little Androscoggin and Androscoggin Rivers. The BioBlitz was a joint project of Maine Conservation Voters and the Androscoggin Land Trust and was conceived of an organized by a USM Environmental Science student interning with Maine Conservation Voters. In this BioBlitz, participants used the citizen science app iNaturalist to photograph wildlife of the river shoreline and add their observations to the app’s database. The Androscoggin BioBlitz project established on the iNaturalist website collected all of …


X-Inactivation And Epigenetics, Serena Weston Apr 2019

X-Inactivation And Epigenetics, Serena Weston

Thinking Matters Symposium Archive

To prevent abnormal development caused by expressing both X chromosomes, female mammals inactivate one of their X chromosomes using an epigenetic process called dosage compensation. This literature review examines how X chromosome inactivation (XCI) occurs during the formation and development of an embryo. This condensation of DNA is marked by histone tail modifications, DNA methylation, and the arrival of structural proteins resulting in extraordinarily stable heterochromatin. X-inactivation is regulated in cis by the X-inactivation center (Xic) that contains the Xist gene and its antisense gene, Tsix. On one X chromosome, Xist RNA is expressed and coats the center of the …


Influence Of Indirect Cues And Vegetation Density On Foraging Behavior In Snowshoe Hares (Lepus Americanus), Zachary Lankist Apr 2019

Influence Of Indirect Cues And Vegetation Density On Foraging Behavior In Snowshoe Hares (Lepus Americanus), Zachary Lankist

Thinking Matters Symposium Archive

Prey avoid chemical cues from predators at feeding sites because the risk of death outweighs the benefit of food. However, we lack information regarding avoidance of chemical cues from competitors as well as how foraging behavior changes alongside vegetative cover. To test if chemical cues and vegetative cover alter prey vigilance, number of visits, and time spent at feeding sites, I observed snowshoe hares (Lepus americanus) in plots containing coyote (Canis latrans; predator) and moose (Alces alces; competitor) urine across a spectrum of vegetation densities. Snowshoe hares significantly reduced the number of visits to feeding plots when coyote or moose …


Exploring The Effects Of Dam Removals On Zooplankton In Penobscot Estuary, Erin Bucci, Karen Wilson Apr 2019

Exploring The Effects Of Dam Removals On Zooplankton In Penobscot Estuary, Erin Bucci, Karen Wilson

Thinking Matters Symposium Archive

Estuaries provide many ecosystem services such as buffering the negative impacts of storms, offering recreation and commercial fishing opportunities, and they serve as a critical habitat corridor for migratory fish. In New England, estuarine habitats have been dramatically altered over the last 200 years due to dam construction; migratory fish such as Atlantic salmon and alewives have suffered. Recently, there have been several dam removal projects in Maine aimed to restore these populations – the largest of these efforts being the Penobscot River Restoration Project. In 2013, two dams were removed from the Penobscot River Estuary, opening more than 2,000 …


Male Determining Gene Nix May Bring An End To Transmission Of Diseases Through Mosquitoes, Mohammed Mahdi Apr 2019

Male Determining Gene Nix May Bring An End To Transmission Of Diseases Through Mosquitoes, Mohammed Mahdi

Thinking Matters Symposium Archive

Female mosquitoes take blood from humans to gain protein needed to produce eggs, and in the process they can transmit viruses, such as Zika, Dengue, and chikungunya, or parasites, such as malaria. Male mosquitoes do not transmit diseases. This literature review will examine what is known about how sex is determined in mosquitoes and how this can be used to manipulate the females that spread diseases to humans. Using CRISPR Cas9, the lab of Zhijian Tu removed the Nix gene from mosquito embryos; all of these were feminized. Ectopic overexpression of Nix gene product in mosquito embryos caused even genetically …


Designing Studies Of Predation Risk For Improved Inference In Carnivore-Ungulate Systems, Mark A. Ditmer Apr 2019

Designing Studies Of Predation Risk For Improved Inference In Carnivore-Ungulate Systems, Mark A. Ditmer

Human-Environment Systems Research Center Faculty Publications and Presentations

Quantifying both the lethal and non-lethal (or “risk”) effects of predation has emerged as a major research focus in carnivore-ungulate systems. While numerous studies have examined predation risk and risk effects in recent decades, a lack of standardization in approaches has impeded progress in the field. We provide an overview of five major study design considerations involved in assessing predation risk and responses of prey in carnivoreungulate systems, highlighting how different design choices can impact the strength and scope of inference. First, we stress the importance of distinguishing measures of predation risk (probability of being killed) from measures of risk …


Climate Change Impacts On Bird Communities Vary Throughout Cities In Kansas, Lindsey Williams, Christine Brodsky Apr 2019

Climate Change Impacts On Bird Communities Vary Throughout Cities In Kansas, Lindsey Williams, Christine Brodsky

Posters

It is important to study climate change’s impacts on biodiversity in order to find a solution before the effects on wildlife are irreversible. Our research objective for this project was to determine if climate change affected long-term bird trends in urban and rural areas throughout Kansas. Our hypothesis was that climate change will decrease bird abundance and species richness because altered climate patterns like storms, droughts, and heat waves, may cause a decrease in suitable habitat. We would expect these trends to be strongest in cities, due to the urban heat island effect. The methods used for this project included …


Camera Trap Study Of The Mammal Communities Across An Urbanization Gradient, Caleb Durbin, Christine Brodsky Apr 2019

Camera Trap Study Of The Mammal Communities Across An Urbanization Gradient, Caleb Durbin, Christine Brodsky

Posters

Urbanization impacts mammal communities due to habitat removal, resource abundance distribution, and the introduction of exotic species. Other studies have found that wildlife species richness is greatest at intermediate urbanization, while abundance is greatest in cities. This study was done to explore the mammal community across an urban to rural gradient in Pittsburg. Using motion-sensor camera traps, we documented small mammal pres­ence for 24 hours a day, finding patterns in mammal abundance, species richness, and community composition. Our hypothesis was that we will observe trends in mammal communities similar to other cities. Over six weeks, we placed two cameras in …


Evaluating The Success Of Female Selected Sex-Sorted Semen At Western Kentucky University's Dairy Farm, Briley Loggan Apr 2019

Evaluating The Success Of Female Selected Sex-Sorted Semen At Western Kentucky University's Dairy Farm, Briley Loggan

Masters Theses & Specialist Projects

The purpose of this study was to evaluate the use of female selected sex-sorted semen and to determine the association of variables on the success of Western Kentucky University’s Dairy Farm. Official breeding and calving records (n=144) were used to determine the relation of lactation number, breeding season, breeding number, breeding year and semen type on pregnancy results, sex of offspring, and the mortality of the offspring. Previous research has shown pregnancy results can be influenced by lactation number, breeding season, number of breedings and semen type. Results from this study show that pregnancy results were not associated with lactation …


Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi Apr 2019

Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi

Masters Theses & Specialist Projects

Antibiotics are used as prophylactic agents to promote growth and for treating infections in animals. However, the irrational use of antibiotics in livestock management is a significant cause of the development of antibioticresistant genes in the environment. Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 of these people are estimated to die because of antibiotic resistance. New drugs are continually coming into the market but are at the risk of developing resistance. Thus, there is a need for the development of analytical methods which can be used to monitor …


Minnesota’S Third Tufted Duck, Philip C. Chu Apr 2019

Minnesota’S Third Tufted Duck, Philip C. Chu

Biology Faculty Publications

No abstract provided.


Genetic Structure Of The Painted Bunting And Its Implications For Conservation Of Migratory Populations, Andrea Contina, Jose L. Alcantara, Eli S. Bridge, Jeremy D. Ross, William F. Oakley, Jeffrey F. Kelly, Kristen Ruegg Apr 2019

Genetic Structure Of The Painted Bunting And Its Implications For Conservation Of Migratory Populations, Andrea Contina, Jose L. Alcantara, Eli S. Bridge, Jeremy D. Ross, William F. Oakley, Jeffrey F. Kelly, Kristen Ruegg

School of Integrative Biological & Chemical Sciences Faculty Publications

The Painted Bunting Passerina ciris is a Neotropical songbird which breeds primarily in the USA during the summer and migrates to Mexico, Central America, southern Florida and the Caribbean over the winter. Male Painted Buntings are brightly coloured, which makes them highly sought after as pets, particularly in Mexico, Central America and Europe. We used short sequence repeats (microsatellite DNA) to investigate the population genetic structure of the Painted Bunting and its implications in conservation management of migratory populations. We found a detectable level of population differentiation as revealed by pairwise FST and RST comparisons and Bayesian clustering …


Guided Alligator Tours Or Raccoon Schooling (Gators), Paul List Apr 2019

Guided Alligator Tours Or Raccoon Schooling (Gators), Paul List

Senior Theses

In my two main internships during college, I worked closely with two very different animals: raccoons and alligators. Additionally, I gained experience in presenting educational programs during my internship at Fripp Island, where my most common program was our Gator Walk. For my thesis project, I built upon my experience in interpretive programming, drawing on my experience with alligators and raccoons to develop a unique educational program to present at Congaree National Park. This report will describe the process of developing this program as well as it’s implementation and outcomes. In addition, it includes a review of scholarly literature on …


A Preliminary Study For The Future Translocation Of A Saimiri Sciureus Troop From Sumak Allpa To Yasuní National Park: Assessing The Habitat Use, Population, And Behavior Of A Common Squirrel Monkey Troop In Indillama, Kenia French Apr 2019

A Preliminary Study For The Future Translocation Of A Saimiri Sciureus Troop From Sumak Allpa To Yasuní National Park: Assessing The Habitat Use, Population, And Behavior Of A Common Squirrel Monkey Troop In Indillama, Kenia French

Independent Study Project (ISP) Collection

Sumak Allpa, an organization specializing in primate rehabilitation, plans to introduce a Common Squirrel Monkey Troop ( Saimiri sciureus ), the Yasuní Troop, to the Indillama region of Yasuní National Park. This study analyzes the habitat use, population, and behavior of the Saimiri sciureus troop, referred to as the Indillama Troop, already existing in the region, from April 14th to May 4th, 2019. Focal and scan observation techniques were used to observe the troop’s behavior, and EasyTrails on an iPhone 7 was used to record GPS data. EasyTrails was also used to map the trail system in the Indillama region. …


Mammals Of The Northern Andes: An Analysis Of Camera Trap Data And Observation In Angochagua, Ecuador, Risa Berman Apr 2019

Mammals Of The Northern Andes: An Analysis Of Camera Trap Data And Observation In Angochagua, Ecuador, Risa Berman

Independent Study Project (ISP) Collection

The high Andes of Ecuador are home to a diverse community of mammals, many of which are threatened. These include the Andean bear, Andean fox, puma, and mountain tapir. This study used camera traps, direct observation, and indirect observation over three weeks to monitor the wildlife in the mountains of the Parroquia of Angochagua. The five species observed during this period were Andean bear, Andean fox, dog, mountain paca, and mouse. While this short study did not completely survey the mammalian community of the area, it confirmed the presence, diet, sociality, temporal habits, elevational range, and habitat preferences of the …


Trends In Nectar Production And Concentration Of Hummingbird-Pollinated Flowers: An Investigation Of Three Flowers Of The Ecuadorian Cloud Forest: Palicourea Demissa, Mezobromelia Capituligera, And Kohleria Affinis, Grace Palmer Apr 2019

Trends In Nectar Production And Concentration Of Hummingbird-Pollinated Flowers: An Investigation Of Three Flowers Of The Ecuadorian Cloud Forest: Palicourea Demissa, Mezobromelia Capituligera, And Kohleria Affinis, Grace Palmer

Independent Study Project (ISP) Collection

Nectar concentration and sugar production both impact and are impacted by pollinator activity through a complex system of coevolution. Additionally, a variety of morphological and environmental factors influence the nectar properties of animal-pollinated flowering plants. Nectar concentration and nectar production rate (NPR) of three hummingbird-pollinated plants, Palicourea demissa, Mezobromelia capituligera, and Kohleria affinis, were measured. These flowers occur and were studied in the southern section of the Chocó-Andean subtropical montane rainforest, in the Pichincha province of Ecuador. Flowers were bagged for 24 hours and sampled at approximate two-hour time intervals for 12 or 24 hours. Nectar concentration and sugar production …


Beach Composition Preferences For Nesting Populations Of Leatherback Sea Turtles (Dermochelys Coriacea), Armila Beach, Guna Yala Comarca, Scott Campbell Apr 2019

Beach Composition Preferences For Nesting Populations Of Leatherback Sea Turtles (Dermochelys Coriacea), Armila Beach, Guna Yala Comarca, Scott Campbell

Independent Study Project (ISP) Collection

Sea turtles play a critical role in marine ecosystems all over the world, including the Caribbean Sea. However, many sea turtle species are under threat due to anthropogenic impacts, such as habitat destruction and fisheries bycatch. This has caused significant declines in sea turtle populations around the world, which in turn has impacted marine ecosystems where sea turtles play critical roles in proper ecosystem functioning. A crucial part of the sea turtle life cycle that has been threatened by anthropogenic factors is nesting. Sea turtles rely on unspoiled beaches with particular physical characteristics for laying their eggs. One of the …


Avian Upsloping In The Tropics: Myioborus Miniatus And Myioborus Torquatus Abundance In Different Altitudinal Ranges In Boquete, Chiriquí, Panama, Julie Yoon Apr 2019

Avian Upsloping In The Tropics: Myioborus Miniatus And Myioborus Torquatus Abundance In Different Altitudinal Ranges In Boquete, Chiriquí, Panama, Julie Yoon

Independent Study Project (ISP) Collection

Direct and indirect effects of warming global temperatures due to climate change are known to cause upwards shifts of the altitudinal ranges of some avian species. Most susceptible to this trend and at risk of riding the “escalator to extinction” are endemic species in tropical montane cloud forests, such as Myioborus torquatus. There are abiotic factors, like temperature, and biotic interactions, such as the presence of its altitudinal neighbor Myioborus miniatus, that limit the altitudinal range of this bird species in the Neotropics. This study measured abundance of M. miniatus and M. torquatus populations at different altitudinal ranges by point …


The Giant Rodents Of Sumak Allpa: A Preliminary Study Of The Ecological Niche Of Amazonian Capybaras In Ecuador, Brynn Furey Apr 2019

The Giant Rodents Of Sumak Allpa: A Preliminary Study Of The Ecological Niche Of Amazonian Capybaras In Ecuador, Brynn Furey

Independent Study Project (ISP) Collection

Sumak Allpa is an island on the Napo River in the eastern rainforest of the Orellana Province of Ecuador that functions as a rehabilitation center for primates. The objective of this investigation is to conduct a preliminary study on the ecological niche of the Amazonian capybaras ( Hydrochoerus hydrochaeris ) that inhabit Sumak Allpa and a small adjacent island known in this study as Island A. Very little information is known about jungle capybaras, especially in Ecuador, and this study aims to establish baseline information about their distribution, diet, and population dynamics. Research was conducted throughout the day, with mornings …


Primates Of The Padangtegal Monkey Forest: Aggressive, Submissive And Affiliative: Interactions Between The Balinese Long-Tailed Macaques And Tourists, Marleigh Jenkins-Morse Apr 2019

Primates Of The Padangtegal Monkey Forest: Aggressive, Submissive And Affiliative: Interactions Between The Balinese Long-Tailed Macaques And Tourists, Marleigh Jenkins-Morse

Independent Study Project (ISP) Collection

Bali is one of Indonesia’s seventeen thousand islands and it has become a major tourist destination for visitors from all over the world. One of the most sought after attractions are the various monkey forests on the island, and one of the most renowned is the Padangtegal Monkey Forest in Ubud. As someone who studies Biological Anthropology, I am fascinated by primates both human and non-human. Within the Ubud Monkey Forest these two species come into contact on a daily basis.

Ethnoprimatology is a growing study, and it refers to the “interconnections between human and nonhuman primates” (McCarthy et al. …


A Survey Of Beetle Diversity (Order Coleoptera) On Lizard Island, John Mccormack Apr 2019

A Survey Of Beetle Diversity (Order Coleoptera) On Lizard Island, John Mccormack

Independent Study Project (ISP) Collection

The beetles (order Coleoptera) of Lizard Island, a small granitic island on the mid shelf of the Great Barrier Reef, have never been assessed in the scientific literature. Prior to our work, only a single beetle genus had been documented on the island (Caryotrypes Decelle, 1968), based on a single specimen collected in 1993 (Reid & Beatson 2013). We conducted a survey of Lizard Island in April 2019 to determine which beetle families are present on the island and which families are the most diverse. The survey also assessed the beetle diversity in different habitats on the island and two …


Regenerative Grazing And The Benefits Of Livestock On Soils In Northern New South Wales, Raymond Mooney Apr 2019

Regenerative Grazing And The Benefits Of Livestock On Soils In Northern New South Wales, Raymond Mooney

Independent Study Project (ISP) Collection

Conventional cattle grazing has received criticism for environmental degradation in the past. Regenerative grazing and the principles of regenerative agriculture show encouraging signs that proper livestock management and planned grazing can reverse degradation and mitigate climate change. An emphasis on soil health and increasing soil carbon and organic matter levels reveals positive feedback for environmental health, the economic security of farmers, and nutritional health of consumers.

In this study I looked to investigate the benefits of regenerative agriculture, reasons why it is being practiced, and the extent it is practiced within the grazing in comparison to traditional methods within Northern …


Iceland's Migratory Birds In Changing Environmental Conditions: An Interactive Synthesis, Frances J. Duncan Apr 2019

Iceland's Migratory Birds In Changing Environmental Conditions: An Interactive Synthesis, Frances J. Duncan

Independent Study Project (ISP) Collection

Human-driven changes to environmental conditions alter the habitats, behaviors, and migration patterns of migratory species. Changes in temperature, vegetation, and precipitation are just some of the factors contributing to shifts in phenology, demography, and distribution of migratory birds. These changes are driven by anthropogenic climate change and amplified by human land-use change, and are especially intense at high latitudes. This project creatively communicates the effects of environmental changes on three species of migratory birds in Iceland—the northern wheatear, the Greenland white-fronted goose, and the black-tailed godwit—using principles of storytelling and game design. The resulting interactive product is a game that …


Eating Some Invasive Species Could Help To Mitigate The Impacts Of Climate Change-Related Invasions, And May Increase Future Food Security, Jesse Bull Saffeir Apr 2019

Eating Some Invasive Species Could Help To Mitigate The Impacts Of Climate Change-Related Invasions, And May Increase Future Food Security, Jesse Bull Saffeir

Independent Study Project (ISP) Collection

Climate change is predicted to increase the spread and abundance of invasive species and to erode global food security. I hypothesized that by incorporating edible invasive species into local food sheds, these two problems could help to mitigate each other. I set out to answer two questions: could eating invasive species reduce their spread and abundance? And could eating invasive species minimize the impacts of climate-change related food shocks? To answer these questions, I surveyed the existing literature on human consumption of invasive species, created a list of criteria that make an invasive species suitable for management through human consumption, …


Microhabitat Comparison Of Percina Roanoka (Roanoke Darter) And Percina Nevisense (Chainback Darter) In The Roanoke River, Dakota R. Spruill, Steven L. Powers Apr 2019

Microhabitat Comparison Of Percina Roanoka (Roanoke Darter) And Percina Nevisense (Chainback Darter) In The Roanoke River, Dakota R. Spruill, Steven L. Powers

Virginia Journal of Science

Snorkel observations of Percina roanoka and P. nevisense in the Roanoke River during summer months were followed by measuring current velocity, water depth, and substrate diameter at points of occupation. A total of 89 observations of P. roanoka and 81 observations of P. nevisense were compared using two-sample T-tests. Percina roanoka inhabited faster, shallower water than P. nevisense with the former found in a mean flow of 0.318 m/s and depth of 31.53 cm and the latter in a mean flow of 0.17 m/s and depth of 55.6 cm. Mean diameter of substrate at points of occupation did not …