Digital Commons Network^™

Performance Of Mycelial Biomass From The Mushroom Ganoderma Lucidum As Feed Additive On Growth And Quality Of Red Hybrid Tilapia (Oreochromis Spp.), Yerima Grema

Student Works (2020-2029)

The present study describes the potentiality of dietary supplement of mycelial biomass of Ganoderma lucidum (MBGL) as functional feed additives formulated along with other feed ingredients at different inclusion levels (5g/kg diet, 10g/kg diet and 15g/kg diet). A hundred and twenty (120) red hybrid tilapia were used for this experiment in duplicates treatment. Fifteen fish were allocated to each tank with an average weight of 17.0g. A six (6) week feeding trial was conducted to evaluate the effect of GL biomass on red hybrid tilapia body composition, organosomatic index, as well as hematological indices. At the end of the feeding …

Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland

Master's Theses

In vitro produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their in vivo counterparts. The aim of this thesis was to alter maturation and culture media to improve the developmental competence of in vitro bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte maturation for 24 hours, fertilization with bull semen for 24 hours, then embryo culture for 7-8 days in 38.5 °C in 5% O …

Survey Of Human-Leopard (Panthera Pardus) Conflict In Ayubia National Park, Pakistan, Muhammad Nabeel Awan, Atif Yaqub, Muhammad Kamran

Journal of Bioresource Management

Wildlife populations are at a risk of extinction mainly because of human-wildlife conflict (HWC). The present study was designed to evaluate the ongoing HWC with special reference to Common Leopard (Panthera pardus) in Ayubia National park through field study as well as a literature-based approach. Questionnaire interview surveys were designed for wildlife officials working in the park and the locals who bear the cost for leopard conflict through livestock depredation and crop damage. The study showed that human-leopard conflict in the study area has been increasing. More than 60% of people considered livestock depredation as the major reason …

Conservation Status Of The Recently Described Ecuadorian Amazon Parrot Amazona Lilacina, Rebecca Biddle, Ivette Solis Ponce, Paul Cun, Simon Tollington, Martin Jones, Stuart Marsden, Christian Devenish, Eric Horstman, Karl S. Berg, Mark Pilgrim

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Amazona lilacina is a threatened species endemic to Ecuador, existing across a patchwork of mangroves, lowland coastal forests, agricultural and community owned land. The species was described in 2014 and listed as ‘Endangered’ on the IUCN Red List, however, full assessment of the population was lacking. Using a combination of field observations, roost surveys and community questionnaires, conducted over the last 20 years, we provide up-to-date information on the species’ Extent of Occurrence, estimate its global population size, and evaluate its level of threat. Our results suggest the species occurs across an area of 19,890 km2 in three distinct geographically …

Feeding Ecology, Behaviour And Habitat Utilization Of Black Drongo (Dicrurus Macrocercus) In Pothwar Plateau, Pakistan, Muhammad Bilal, Zeeshan Khalid, Ali Hasnain Mosvi, Amir Naseer

Journal of Bioresource Management

Black Drongo is an insectivorous bird native to Pakistan. The present study focused on its feeding preferences, behaviour, ecology and habitat relationships. The study was carried out from first week of December, 2019 to the second week of April, 2020 in the two villages of Rawalpindi named as Daultala (Location-I) and Nata Gujarmall (Location-II). Observations were made by point count method on foot using line transect method. Air (52.27%) was the most commonly used feeding substrate. Aerial feeding mode (52.27%) is preferred over plant or tree gleaning (n=58) and ground feeding (n=47). Most common perching site was man made obstacle, …

Integrated Management Of Aphis Craccivora In Cowpea Using Intercropping And Entomopathogenic Fungi Under Field Conditions, Allan Mweke, Komivi Senyo Akutse, Christian Ulrichs, Komi Kouma Mokpokpo Fiaboe, Nguya Kalemba Maniania, Sunday Ekesi

All Peer-Reviewed Publications

Cowpea aphid, Aphis craccivora, is a major cowpea pest. Cowpea–cereal intercrop alone does not effectively manage the pest. Use of pesticides in intercrop leads to health and environmental risks. Fungal-based biopesticides offer a better option because they are environment-and consumer-friendly. This study assessed the combined effect of Metarhizium anisopliae ICIPE 62 and cowpea–maize intercrop against A. craccivora under six treatments: (1) untreated cowpea monocrop, (2) untreated cowpea–maize intercrop, (3) cowpea monocrop + ICIPE 62, (4) cowpea–maize intercrop + ICIPE 62, (5) cowpea monocrop + Duduthrin insecticide, and (6) cowpea–maize intercrop + Duduthrin during three seasons (long rainy/cold and dry/short rainy). …

Type I Interferon Underlies Severe Disease Associated With Junín Virus Infection In Mice, Brady T. Hickerson, Eric J. Sefing, Kevin W. Bailey, Arnaud J. Van Wettere, Manuel L. Penichet, Brian B. Gowen

Animal, Dairy, and Veterinary Science Student Research

Junín virus (JUNV) is one of five New World mammarenaviruses (NWMs) that causes fatal hemorrhagic disease in humans and is the etiological agent of Argentine hemorrhagic fever (AHF). The pathogenesis underlying AHF is poorly understood; however, a prolonged, elevated interferon-α (IFN-α) response is associated with a negative disease outcome. A feature of all NWMs that cause viral hemorrhagic fever is the use of human transferrin receptor 1 (hTfR1) for cellular entry. Here, we show that mice expressing hTfR1 develop a lethal disease course marked by an increase in serum IFN-α concentration when challenged with JUNV. Further, we provide evidence that …

Genetic Biocontrol For Invasive Species, John L. Teem, Luke Alphey, Sarah Descamps, Matt P. Edgington, Owain Edwards, Neil Gemmell, Tim Harvey-Samuel, Rachel L. Melnick, Kevin P. Oh, Antoinette J. Piaggio, J. Royden Saah, Dan Schill, Paul Thomas, Trevor Smith, Andrew Roberts

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive species are increasingly affecting agriculture, food, fisheries, and forestry resources throughout the world. As a result of global trade, invasive species are often introduced into new environments where they become established and cause harm to human health, agriculture, and the environment. Prevention of new introductions is a high priority for addressing the harm caused by invasive species, but unfortunately efforts to prevent new introductions do not address the economic harm that is presently manifested where invasive species have already become established. Genetic biocontrol can be defined as the release of organisms with genetic methods designed to disrupt the reproduction …

Authentication Of Red Snapper (Lutjanus Campechanus) Fillets Using A Combination Of Real-Time Pcr And Dna Barcoding, Rachel B. Isaacs, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Red snapper (Lutjanus campechanus) is a historically overfished and highly valued species that is commonly substituted with other fish, such as tilapia, rockfish, and other snapper species. The objective of this study was to assess the ability of real-time PCR to be used as a screening tool to rapidly test commercial fillets for the presence of red snapper, followed by species identification of negative samples with DNA barcoding. A total of 24 frozen, fresh, or thawed (previously frozen) fillets labeled as “red snapper” were tested with real-time PCR, along with 54 fillets from fish that are common substitutes …

Air Temperature, Carbon Dioxide, And Ammonia Assessment Inside A Commercial Cage Layer Barn With Manure-Drying Tunnels, W Zheng, Y Xiong, R S. Gates, K W. Koelkebeck

Department of Animal Science: Faculty Publications

Understanding the air temperature distribution, ammonia (NH₃) and carbon dioxide (CO₂) levels in poultry housing systems are crucial to poultry health, welfare, and productivity. In this study, 4 Intelligent Portable Monitoring Units and 7 temperature sensors were installed inside and between the cages and above 2 minimum ventilation fans of a commercial stacked-deck cage laying hen house in the Midwest United States (425,000 laying hens) to continuously monitor the interior environment over a 6-month period. During cold conditions (March 12th–May 22nd), there was a variation noted, with barn center temperatures consistently being highest in the longitudinal …

Physiological And Behavioral Correlates Of Hif-1 Alpha Protein Levels In The Gulf Killifish, Fundulus Grandis, Jasmine Harris

LSU New Orleans Theses and Dissertations

Although the hypoxia-inducible transcription factor (HIF) orchestrates molecular responses to low oxygen, the amount of HIF-1α protein expressed during hypoxia varies among species, individuals, and tissues. This study measured HIF-1α protein levels along with several physiological and behavioral variables in the estuarine fish Fundulus grandis under normoxia (> 7 mg l^-1 dissolved oxygen) and hypoxia (1 mg l^-1 dissolved oxygen). Fish under hypoxic conditions had higher tissue levels of HIF-1α, hematocrit, blood glucose, blood lactate, frequency of aquatic surface respiration (ASR), and lower activity than normoxic controls. Under hypoxia, HIF-1α abundance in gill was positively correlated with body …

Reviewing The Potential Link Between Grain-Free Diets And Dilated Cardiomyopathy In Canines, Larissa J. Webster

University Honors Theses

Grain-free diets for dogs are becoming increasingly popular, but concerns are emerging following the FDA's recent investigation that there might be a link between grain-free diets and Dilated Cardiomyopathy (DCM) in dogs with no known genetic predisposition. Contrary to the majority of the public thought, dogs do nutritionally require grains, and there is rarely a medical reason to switch to a grain-free diet. Not all cases of DCM are linked to diet, and many dogs who are fed grain-free diets do not develop the disease; the extent of this issue therefore is not entirely known. This review aims to explore …

A Methodological Review Of Resilience Approaches For Coral In The Face Of Climate Change, Gabriella L. Martinez

University Honors Theses

A literature review of research on Resilience Based Management Theory, Aquaculture, and Assisted Gene Flow and Evolution being explored as possible methods to help the coral bleaching crisis.

Development, Characterization, And Application Of Two Reporter-Expressing Recombinant Zika Viruses, Sang-Im Yun, Byung-Hak Song, Michael E. Woolley, Jordan C. Frank, Justin G. Julander, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV), a mosquito-borne transplacentally transmissible flavivirus, is an enveloped virus with an ~10.8 kb plus-strand RNA genome that can cause neurological disease. To facilitate the identification of potential antivirals, we developed two reporter-expressing ZIKVs, each capable of expressing an enhanced green fluorescent protein or an improved luminescent NanoLuc luciferase. First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. Two reporter-expressing ZIKVs were then generated by …

Scorpions Of Puerto Rico And Its Satellite Islands (Scorpiones: Buthidae, Diplocentridae): An Аnnotated List, Key For Genera, And Bibliography, Luis De Armas

Euscorpius

An annotated list of the scorpions of Puerto Rico and its satellite islands (mainly Vieques, Culebra, Caja de Muertos, Magueyes, Desecheo, and Mona), a key for the seven genera present in those insular territories, a key for the three species of Microtityus, and the basic bibliography on taxonomy and natural history are provided. Of the 16 confirmed species described from these Greater Antillean islands, four are known only from a single sex and only one or two adult specimens. Seven species (six of them from the genus Tityus), are endemic to the Puerto Rico Island; Vieques has two …

Size Selective Parasitism Of Rainbow Trout (Oncorhynchus Mykiss) By Chestnut Lampreys (Ichthyomyzon Castaneus) In An Artificial Setting, Jeremiah M. Salinger, Ronald L. Johnson

Southeastern Fishes Council Proceedings

Paradigms of optimal resource utilization by animals, both classical and more recent, were not originally developed in the context of parasitism. Though this oversight has slowly been reversed, little attention has been paid to optimal resource utilization by parasitic fishes, such as lampreys. Multiple explanations for host size selection by parasitic lampreys may be plausible, but results from previous studies have been inconsistent. We studied host size selection by Chestnut Lampreys (Ichthyomyzon castaneus) parasitizing Rainbow Trout (Oncorhynchus mykiss) in fish hatchery raceways in north central Arkansas during the late winter and early spring of 2013. Parasitized …

Movement Of Translocated Adult Sicklefin Redhorse (Moxostoma Sp.) In The Oconaluftee River, North Carolina: Implications For Species Restoration, Jessica L. Davis, David P. Gillette, C. Reed Rossell Jr., Michael J. Lavoie

Southeastern Fishes Council Proceedings

The Sicklefin Redhorse is a rare, undescribed species of Moxostoma, endemic to the Hiwassee and Little Tennessee River basins of western North Carolina and northern Georgia, where it has been eliminated from much of its native range. It is listed as endangered in Georgia and threatened in North Carolina. Although it has not been granted federal protected status, this species is the subject of a Candidate Conservation Agreement between federal, state, tribal, and private stakeholders, of which one objective calls for the re-establishment of Sicklefin Redhorse populations throughout its historical range. The objective of our study was to evaluate …

Proposal For Comparative Morphometrics Of Tübingen (Tu) And Tübingen Longfin (Tl) Zebrafish (Danio Rerio), Andrew Draper

University Honors Theses

The TL strain of zebrafish (Danio rerio) originated from the TU strain, and differs in fin pattern with a phenotype predominated by increased fin length. Two genes are known to affect the phenotype: leo and lof. The pattern-affecting leo gene is not believed to affect the growth rate, development, or proportionality of the body. The lof gene does however result in significant increase in fin length compared to the wild-type phenotype of TU. Using populations from both TL and TU genetic strains, development was observed from 48 hours post fertilization through sexual maturity at 90 days post fertilization …

Status Of The Blackstripe (Fundulus Notatus) And Blackspotted (F. Olivaceus) Topminnows In The Ozark Uplands Of Central Missouri, Nathaniel Steffensmeier, Naznin Sultana Remex, Robert Hrabik, David D. Duvernell

Southeastern Fishes Council Proceedings

The topminnow species Fundulus notatus and F. olivaceus have broadly overlapping geographic distributions that extend throughout much of the central and southern United States. In the northern portion of their respective ranges, in Missouri, the regional distributions of the two species coincide largely with recognized ecoregions. In the unglaciated southern half of Missouri, F. olivaceus is distributed throughout Ozark upland habitats while F. notatus is abundant in marginal large river and prairie habitats along the Ozark borders. An exception to this partitioning is the historical report of abundant F. notatus in the Bourbeuse and upper Meramec River drainages within the …

Use Of Dead Mussel Shells By Madtom Catfishes In The Green River, Jacob F. Brumley, Philip W. Lienesch

Southeastern Fishes Council Proceedings

The Green River in Kentucky has high fish and macroinvertebrate diversity. As both fish and macroinvertebrates have evolved together in this system, relationships have developed between species. One type of relationship that has been observed is between madtom catfishes (Noturus spp.) and mussels in the Green River, where madtoms use dead mussel shells as cover when not actively foraging. In the fall of 2016 and 2017, surveys were conducted to determine if madtom catfishes use dead mussel shells more than rocks of similar size. We predicted that madtoms would select mussel shells as cover more frequently than rocks due …

The Gut Microbiome And Its Potential Role In Paradoxical Anaerobism In Pupfishes Of The Mojave Desert, Brian Hedlund

Life Sciences Faculty Research

Background: Pupfishes frequently enter paradoxical anaerobism in response to endogenously produced or exogenously supplied ethanol in a dose-dependent manner. To decipher the role of the gut microbiota in ethanol-associated paradoxical anaerobism, gut microbial communities were depleted using a cocktail of antibiotics and profiled using 16S rRNA gene sequencing. Results: Compared to the control group (n = 12), microbiota-depleted fish (n = 12) spent more time in paradoxical anaerobism. Our analysis indicated that the bacterial phyla Proteobacteria, Fusobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Patescibacteria, and Dependentiae dominated the pupfish gut, which is consistent with other fish gut microbiota. Although the gut microbial communities …

A New Species Of Isometrus (Scorpiones: Buthidae) From Southern India, Shauri Sulakhe, Nikhil Dandekar, Shomen Mukherjee, Malay Pandey, Makarand Ketkar, Anand Padhye, Deshabhushan Bastawade

Euscorpius

A new species of Isometrus (Buthidae) is described from India using integrated taxonomic approach. Isometrus kovariki sp. n. is closely related to I. thurstoni, and differs in morphological features and raw genetic divergence of more than 9%.

The Effects Of Auditory Predator Cues On Foraging Patterns In Peromyscus Polionotus, Christina L. Sartain

Honors College Theses

Abstract:

While it is established that animals react to predator cues, little research has been done to assess its effect on foraging patterns and behavior. Foraging patterns are determined by a variety of factors associated with perceived predation risk, such as vegetation cover and moonlight exposure. Predator cues come in many forms, both direct and indirect, and are another indicator of perceived predation risk. Past research suggests that oldfield mice, Peromyscus polionotus, may react to indirect cues (i.e vegetation cover) more often than some direct cues, such as urine of a predator. Other indirect cues, such as vegetation cover and …

Anchialine Biodiversity In The Turks And Caicos Islands: New Discoveries And Current Faunal Composition, Brett C. Gonzalez, Alejandro Martínez, Jørgen Olesen, Sarit B. Truskey, Lauren Ballou, Marc Allentoft-Larsen, Joost Daniels, Paul Heinerth, Mark Parrish, Naqqi Manco, Jon Ward, Thomas M. Iliffe, Karen J. Osborn, Katrine Worsaae

International Journal of Speleology

Lying at the southernmost point of the Lucayan Archipelago, the Turks and Caicos Islands are amongst the better studied localities for anchialine cave biodiversity. For nearly five decades, novel invertebrate fauna, comprised primarily of crustaceans, have been collected from these tidally influenced pools – but new findings are always on the horizon. Herein we present new records of crustaceans and annelids from anchialine blue holes and horizontal caves of the Turks and Caicos. These findings include two potentially new species of meiofaunal annelids and a new species of remipede collected from a shallow water cave pool. Our 2019 expedition additionally …

Influence Of Total Western Diet On Docosahexaenoic Acid Suppression Of Silica-Triggered Lupus Flaring In Nzbwf1 Mice, Kristen N. Gilley, Kathryn A. Wierenga, Preeti S. Chauhaun, James G. Wagner, Ryan P. Lewandowski, Elizbeth A. Ross, A. L. Lock, Jack R. Harkema, Abby D. Benninghoff, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Lupus is a debilitating multi-organ autoimmune disease clinically typified by periods of flare and remission. Exposing lupus-prone female NZBWF1 mice to crystalline silica (cSiO₂), a known human autoimmune trigger, mimics flaring by inducing interferon-related gene (IRG) expression, inflammation, ectopic lymphoid structure (ELS) development, and autoantibody production in the lung that collectively accelerate glomerulonephritis. cSiO₂-triggered flaring in this model can be prevented by supplementing mouse diet with the ω-3 polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA). A limitation of previous studies was the use of purified diet that, although optimized for rodent health, does not reflect the …

Photographic Validation Of Target Versus Nontarget Take Of Brown Treesnake Baits, Shane R. Siers, Aaron B. Shiels, Cynthia G. Payne, Francinem M. Chlarson, Craig S. Clark, Stephen M. Mosher

United States Department of Agriculture Wildlife Services: Staff Publications

Use of toxic baits or other tools for managing nuisance species must ensure that the species of interest is adequately targeted while exposure to nontarget species is minimized. Nontarget takes of acetaminophen‐laced baits for control of invasive brown treesnakes (Boiga irregularis) on Guam may put those animals at risk of lethal intoxication and render the bait unavailable to the intended target species. We used wildlife cameras to identify species removing toxic and nontoxic baits from brown treesnake bait stations designed to exclude nontarget taxa in 2015 and 2016. Throughout various sites and habitat types, and balanced by season (wet vs. …

Time Allocation To Resources By Three Species Of Rats (Rattus Spp.) In A Radial Arm Maze, Gary Witmer, Nathan P. Snow, Rachael S. Moulton

United States Department of Agriculture Wildlife Services: Staff Publications

Context. Introduced rats (Rattus spp.) can pose a serious threat to native flora and fauna, especially on islands where most species have evolved in the absence of terrestrial predators. Effective detection and eradication methods for introduced rats are essential to the maintenance of insular ecosystem integrity. Thus, it is important to better understand the behaviour of rats when they first arrive in a new setting.

Aims. To determine whether rats would find some novel stimuli to be significantly more attractive than other novel stimuli.

Methods. An eight-arm radial maze was used to study the behaviour of three species of Rattus …

Interactions With Humans Shape Coyote Responses To Hazing, Julie K. Young, Edd Hammill, Stewart W. Breck

United States Department of Agriculture Wildlife Services: Staff Publications

Medium and large carnivores coexist with people in urban areas globally, occasionally resulting in

negative interactions that prompt questions about how to reduce human-wildlife conflict. Hazing,

i.e., scaring wildlife, is frequently promoted as an important non-lethal means for urbanites to reduce

conflict but there is limited scientific evidence for its efficacy. We used a population of captive coyotes (Canis latrans) to simulate urban human-coyote interactions and subsequent effects of hazing on coyote behavior. Past experiences with humans significantly affected the number of times a coyoteapproached a human to necessitate hazing. coyotes that had been hand fed by adults had to …

Hawaii As A Microcosm: Advancing The Science And Practice Of Managing Introduced And Invasive Species, Liba Pejchar, Christopher A. Lepczyk, Jean E. Fantle-Lepczyk, Steven C. Hess, M. Tracy Johnson, Christina R. Leopold, Michael Marchetti, Katherine M. Mcclure, Aaron B. Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive species are a leading driver of global change, with consequences for biodiversity and society. Because of extraordinary rates of endemism, introduction, and extinction, Hawaii offers a rich platform for exploring the cross-disciplinary challenges of managing invasive species in a dynamic world. We highlight key successes and shortcomings to share lessons learned and inspire innovation and action in and beyond the archipelago. We then discuss thematic challenges and opportunities of broad relevance to invaded ecosystems and human communities. Important research needs and possible actions include eradicating mammals from mainland island sanctuaries, assessing hidden threats from poorly known introduced species, harnessing …

Deciphering Interactions Between White-Tailed Deer And Approaching Vehicle, Morgan Pfeiffer, Raymond B. Iglay, Thomas W. Seamans, Bradley F. Blackwell, Travis L. Devault

United States Department of Agriculture Wildlife Services: Staff Publications

Deer-vehicle collisions are a major transportation hazard, but factors affecting deer escape decision-making in response to vehicle approach remain poorly characterized. We made opportunistic observations of deer response to vehicle approach during daylight hours on a restricted- access facility in Ohio, USA (vehicle speeds were ≤64 km/h). We hypothesized that animal proximity to the road, group size, vehicle approach, and ambient conditions would affect perceived risk by white-tailed deer (Odocoileus virginianus) to vehicle approach, as measured by flight-initiation distance (FID). We constructed a priori models for FID, as well as road-crossing behavior. Deer responses were variable and did not demonstrate …