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Effect Of Retinoic Acid On The Expression And Function Of Ap-1 Transcription Factor In B16 Mouse Melanoma Cells: Role Of Protein Kinase, Sejal H. Desai

Theses, Dissertations and Capstones

Retinoic acid (RA) induces differentiation of B16 mouse melanoma cells. This differentiation is accompanied by an increase in protein kinase Ca (PKCα) protein level and selective enrichment in nuclear-associated PKCα. PKC is thought to regulate gene expression through the TPA response element (TRE). This element is specifically recognized by the AP-1 transcription factor composed of jun and fos family members. In this study, I have analyzed the effect of RA on the expression and function of AP-1 in B16 mouse melanoma cells. Transient transfection analysis of B16 cells using leuciferase reporter gene constructs with or without AP-1 elements indicated that …

Regulation Of Melanogenesis In B16 Mouse Melanoma Cells By Protein Kinase C (Pkc), Harish Mahalingam

Theses, Dissertations and Capstones

The pigment cell-specific expression of tyrosinase and TRP1 has been shown to be important for the production of melanin in pigmented cells. Using a pigmented cell line, B16 mouse melanoma, we obtained evidence that PKC plays a major role in regulating melanogenesis. Chronic treatment with phorbol dibutyrate (PDBu) leads to downregulation of PKC activity and protein levels. This is accompanied by a loss of pigmentation which is correlated with a 50% reduction and a complete loss of TRP1 and tyrosinase respectively. Similar results were obtained with Northern and Western blotting indicating that PKC may regulate the steady state levels of …

Genetic Analysis Of Rhinichthys Atratulus (Pisces: Cyprinidae) In North Central West Virginia, Alan Harper Tennant

Theses, Dissertations and Capstones

The blacknose dace, Rhinichthys atratulus, is a cyprinid minnow common to shallow streams throughout North America. It has been shown to be sensitive to anthropogenic stress and a study of the genetic nature of R. atratulus populations may yield information about their habitats. In West Virginia, two subspecies of R. atratulus are present, R. a. atratulus and R. a. obtusus, which are separated by the Allegheny divide. In north central West Virginia, R. a. atratulus was observed in the Cheat River drainage, an area previously thought be within the range of R. a. obtusus. Fish of the R. a. atratulus …

Grouse News 11-20 (1996-2000), World Pheasant Association International, Diana Lovel

Galliformes Specialist Group and Affiliated Societies: Newsletters

Issue 11 (June 1996)

Editorial, Diana Lovel

Diary of David and Margaret's visit to the Pyrenees June 19-28, 1995

Adaptability of gamebirds, David Jenkins

Pheasants and grouse, Matt Ridley

Letter to the editor, Tim Lovel

Grouse Group founded, Rudi Suchant

Hybrids between capercaillie and black grouse and their characteristics, Jan Porkert

Does the occurrence of capercaillie-black grouse hybrids in Øvre Landvik, southern Norway signalize the coming extinction of capercaillies, as it did in Ostsudeten in the Czech Republic? Jan Porkert in cooperation with Arne Flor

Review of Naturschutz report

The effect of matrix on the occurrence of hazel grouse in …

Factors Affecting Interannual Movements Of Snowy Plovers, Peter W. C. Paton, Thomas C. Edwards Jr.

Natural Resources Science Faculty Publications

We studied the interannual movements of 361 individually color-banded adult Snowy Plovers (Charadrius alexandrinus nivosus) at Great Salt Lake, Utah from 1990 to 1993. In northern Utah, Snowy Plovers nested in a dynamic environment; suitable breeding habitat declined by 50% at two study areas in four years. Male Snowy Plovers were more site faithful than females; 40% of males exhibited fidelity compared with 26% of females (P < 0.01). However, as the amount of available suitable nesting habitat declined, male site fidelity diminished, whereas female fidelity remained relatively constant. We found strong evidence that female site fidelity was affected by nesting success in the previous year. Females that nested unsuccessfully were less likely than successful females to exhibit site fidelity the following year; males did not exhibit this nest-success bias. In addition, unsuccessful females breeding at sites with high densities of nests tended to disperse the following year, whereas male site fidelity did not appear to be affected by either a study site's overall nesting success the previous year or a study site's nest density the previous year. Female avoidance of areas with high densities of nests may be an antipredator strategy. Snowy Plovers in northern Utah have biparental incubation duties, but only males care for broods. Familiarity with brood-rearing areas was one plausible explanation for male-biased fidelity. However, we could not eliminate an alternative hypothesis that both focal study sites represented scarce breeding areas due to the presence of freshwater, and male Snowy Plovers preferred to use the same areas rather than disperse. We propose that more landscape-level studies are needed to address questions concerning local and regional movement patterns.

Overhead Wires Reduce Roof-Nesting By Ring-Billed Gulls And Herring Gulls, Jerrold L. Belant, Sheri K. Ickes

Vertebrate Pest Conference Proceedings: 17th (1996)

The authors evaluated the effectiveness of overhead wires in reducing roof-nesting by ring-billed gulls (Larus delawarensis) and herring gulls (L. argentatus) at a 7.2 ha food warehouse in Bedford Heights, Ohio during 1994-1995. In 1994, stainless steel wires (0.8 mm diameter) were attached generally in spoke-like configurations between 2.4 m upright metal poles spaced at 33.7 m intervals over the main portion of roof. The 6 to 14 wires radiating from each pole created a mean maximum spacing between wires of about 16 m. Nesting by ring-billed and herring gulls was reduced by 76% and 100% …

Towards "Best Practice" Vertebrate Pest Management In Australia, Mike Braysher, Peter O'Brien, Mary Bomford

Vertebrate Pest Conference Proceedings: 17th (1996)

Australia has 26 species of introduced pest mammals that cause extensive damage to agriculture and the conservation of native wildlife. Past efforts tried to eradicate them. This focus on reducing pest numbers rather than the outcome, reduced damage, has had limited success. Under its Vertebrate Pest Program, the Bureau of Resource Sciences has developed principles and a strategic approach to managing pest damage. Close cooperation with land managers as co-researchers and co-learners is an essential element, as is a coordinated group approach to pest management. The approaches are illustrated with an example.

An Overview Of Animal Damage Control (Adc) Assistance To The Vertebrate Pest Management Industry, William H. Clay

Vertebrate Pest Conference Proceedings: 17th (1996)

The Animal Damage Control (ADC) program has had a long history dating back to 1885. ADC was officially established in 1931 under the United States Department of Agriculture. In 1939, the program was moved to the United States Department of Interior's Fish and Wildlife Service. In 1996, ADC was transferred back to the USDA and placed under the Animal and Plant Health Inspection Service. The mission of the Animal Damage Control program is to provide federal leadership in managing problems caused by wildlife. Current program assistance includes: a) technical assistance in wildlife management; b) conducting research and development activities related …

Impacts Of A Daily Trap Check Law On The California Adc Program, Craig Coolahan

Vertebrate Pest Conference Proceedings: 17th (1996)

Effective January 1, 1990 California law required that all steel-jawed leghold traps be inspected at least daily and all animals in such traps be removed. The inspection and removal could be performed by the individual who set the traps, the landowner, or an agent of either. Prior to the passage of this law, California Animal Damage Control (ADC) personnel were exempt from Department of Fish and Game trap checking regulations. The data suggest that a decrease in trap use occurred after the implementation of the daily trap check. Where the program could effectively substitute other control tools or methods for …

Analysis Of Vertebrate Pest Research, Jim Hone

Vertebrate Pest Conference Proceedings: 17th (1996)

Research on vertebrate pest control is mostly empirical, focusing on control of species X in location Y using method Z. Such an approach is needed. The science of vertebrate pest research is developing some generalizations across species, locations, and methods. This paper further explores such generalizations by discussing six questions asked by Hone (1994), the answers to which are relevant to vertebrate pest research world-wide. Several case studies are examined, with emphasis on control of damage by small mammals and predation control. Suggestions are made for future research.

Operational Control Of The Brown Tree Snake On Guam, Thomas C. Hall

Vertebrate Pest Conference Proceedings: 17th (1996)

An operational control program for brown tree snakes (Boiga irregularis) on Guam began in April 1993. The program focused on minimizing the dispersal of brown tree snakes to other Pacific islands and the U.S. mainland. During the first year of operation, more than 3,000 snakes were caught within a kilometer of high risk port facilities using traps, detector dogs, and spotlighting. Additionally, habitat modifications and prey-base removal were used to reduce the attractiveness of these facilities to brown tree snakes. Public awareness was also an important part of the program such as the education of cargo packers, shippers, …

Rodents And Cover Crops—A Review, Gregory A. Giusti, Desley A. Whisson, W. Paul Gorenzel

Vertebrate Pest Conference Proceedings: 17th (1996)

Inter-row plantings of herbaceous cover crops has become a widely accepted practice by orchard and vineyard managers. Cover crops, used as part of a production management system, are not considered a cash crop and are therefore selected by individual growers for various reasons. Little is written regarding the relationship of cover crop management and the impact on rodent populations. This paper reviews the recent literature and examines how cover crop species and cultivar selection along with management procedures may be influential in limiting rodent populations and their damage to cropping systems.

The Persistence And Secondary Poisoning Risks Of Sodium Monofluoroacetate (1080), Brodifacoum, And Cholecalciferol In Possums, C. T. Eason, G. R. Wright, L. Meikle, P. Elder

Vertebrate Pest Conference Proceedings: 17th (1996)

To determine the risk of secondary poisoning for animals preying on sub-lethally poisoned brushtail possums, captive possums were treated with near-lethal doses of sodium monofluoroacetate (1080) or brodifacoum, and toxicant concentrations in blood and tissue were monitored over time. Sodium monofluoroacetate was rapidly eliminated from the blood (within three days). Brodifacoum was retained in the liver and, to a lesser extent, the muscle of possums for eight months after dosing. To determine the potential risk for animals scavenging on the carcasses of possums poisoned with cholecalciferol, cats were fed poisoned carcasses for six days. No changes in behavior, appetite, or …

The Use Of Tip Traps To Control Rabbit Damage In Scotland, Robert M. E. Fuchs, W. Kenneth Maclean, Caroline A. Mackintosh, Iain M. Allan

Vertebrate Pest Conference Proceedings: 17th (1996)

The factors affecting efficient use of tip traps to control rabbit populations were investigated in a series of field experiments. It was found that continual trapping at the same location was much less effective than periodic trapping. Night-time trapping operations produced larger catches of rabbits than day-time trapping. Traps were equally effective whether sited on existing runs through rabbit proof fences or on previously unbreached sections of fence. The sex ratio of rabbits caught was examined at four different locations and, in each instance, more females were caught than males. The installation of a network of tip traps and associated …

Perceptions Of Wildlife Damage By Conservation Reserve Program Contract Holders In Riley County, Kansas, John P. Hughes, Philip S. Gipson

Vertebrate Pest Conference Proceedings: 17th (1996)

Twenty-five Conservation Reserve Program (CRP) contract holders in Riley County, Kansas were surveyed by telephone to assess their perceptions of wildlife damage relative to CRP plantings. Sixty-four percent experienced wildlife damage on their farm or ranch. Respondents felt that five species causing damage on their farm or ranch had become more common due to enrollment of lands in the CRP. White-tailed deer (Odocoileus virginianus) accounted for 64.3% of these observations, followed by wild turkey (Meleagris gallopavo), eastern cottontail (Sylvilagus floridanus), striped skunk (Mephitis mephitis), and Virginia opossum (Didelphis virginiana), which accounted for …

The Potential Impact Of Introduced Commensal Rodents On Island Flora, Gillian Key, Renata Platenberg, Andrew Easby, Kathleen Mais

Vertebrate Pest Conference Proceedings: 17th (1996)

The impact of introduced commensal rodents on island flora has been relatively little studied compared with their impact on the fauna. The effects on vegetation composition, regeneration, and decomposition are largely unknown, but potentially great. Preliminary studies were carried out in the Galapagos Islands between 1993 and 1994 on the diet of introduced rats, Rattus spp. and feral house mice, Mus musculus, seed recovery rates and subsequent germination rates of seeds. R. rattus diet was primarily vegetation and 48% of rats had seeds in their stomachs. Significant differences were found between body size and overall contribution of both …

Mole Control-A Historical Perspective, Rex E. Marsh

Vertebrate Pest Conference Proceedings: 17th (1996)

Various methods and approaches, including chemical and physical repellents, flooding, burrow fumigants, poison baits, vibrating devices and exclusion, have been explored for reducing mole problems. In addition to these, habitat management through reducing the moles food supply has received considerable attention, but environmental concerns and the lack of consistent results have tempered this approach. Over the years, trapping remains the best and most useful method of mole control. The pros and cons of some of the methods are discussed, along with some historical perspectives. The emphasis is placed on the Broad-footed mole, Scapanus latimanus, of California.

Results Of A Non-Lethal Survey And Report Provided To The New Mexico Legislature, J. Alan May

Vertebrate Pest Conference Proceedings: 17th (1996)

Social and political pressures affect decision making regarding wildlife damage management issues tremendously. In fact, these areas are included in the Animal Damage Control decision model outlined in the programmatic Environmental Impact Statement. Growing concern regarding pain and suffering of animals trapped by ADC Specialists prompted two actions by the 41st Legislature of the State of New Mexico in 1994. The legislature directed New Mexico ADC not to spend over three-quarters of its $304,000 appropriation on lethal methods. The legislature also passed a memorial bill requesting the New Mexico Department of Game and Fish, in cooperation with the U.S. Fish …

Conference Participants

Vertebrate Pest Conference Proceedings: 17th (1996)

The number of registered attendees was 340. The participants came from 31 states, the District of Columbia, and from 9 other countries. The wide representation from the United States and countries throughout the world contributed to the success of the Conference by providing a highly knowledgeable and diversified group for the exchange of research progress, new ideas, and information on a wide range of vertebrate pest topics.

Preliminary Study Of The Genetics Of Resistance In The House Mouse, Colin V. Prescott

Vertebrate Pest Conference Proceedings: 17th (1996)

A wild house mouse (Mus domesticus) population originally trapped near Reading, Berkshire, United Kingdom, and maintained as a colony in the laboratory, was subjected to the discriminating feeding period of the warfarin resistance test, as used by Wallace and MacSwiney (1976) and derived from the work of Rowe and Redfern (1964). Eighty percent of this heterogeneous population survived the resistance test. A similar proportion of the population was found to survive the normally lethal dose of bromadiolone administered by oral gavage. The majority of this population of mice were classified as "warfarin-resistant" and "bromadiolone-resistant." The dose of l0 …

Palatability Of Rodenticide Baits In Relation To Their Effectiveness Against Farm Populations Of The Norway Rat, Roger J. Quy, David P. Cowan, Colin Morgan, Tom Swevney

Vertebrate Pest Conference Proceedings: 17th (1996)

The palatability of 12 rodenticide baits, formulated to vary from poorly accepted to well accepted, was measured in laboratory choice tests against Wistar and wild-caught Norway rats. The baits, derived from six bait bases and two active ingredients, difenacoum and bromadiolone, were simultaneously tested in the field against 24 farm infestations (2/formulation) in order to investigate the relationship between palatability and efficacy. Bait acceptance in laboratory tests, with EPA meal as the challenge diet, varied from 7.0 to 50.6% for Wistar rats and 3.7 to 85.1 % for wild rats. Changing the challenge diet to a ground-up laboratory animal food …

The Evolution Of Aphis Two Gas Cartridges, Craig A. Ramey, Edward W. Schafer Jr

Vertebrate Pest Conference Proceedings: 17th (1996)

The U.S. Department of Agriculture, Animal and Plant Health Inspection Service (APHIS) has two federal (Section 3) vertebrate pesticide registrations with the U.S. Environmental Protection Agency (EPA) for gas cartridges to control damage to American agricultural resources and reduce threats to public health and safety. The gas cartridges are pyro-fumigant devices that produce primarily carbon monoxide when ignited. In sealed burrows or dens, carbon monoxide is highly toxic when inhaled, leading to tissue hypoxia. Carbon monoxide is recommended by the American Veterinary Medicine Association's panel for euthanatizing animals because it quickly induces unconsciousness without pain and with minimal discernible discomfort. …

Population Densities And Disease Surveys Of Wild Pigs In The Coast Ranges Of Central And Northern California, Rick A. Sweitzer, Ian A. Gardner, Ben J. Gonzales, Dirk Van Vuren, Walter M. Boyce

Vertebrate Pest Conference Proceedings: 17th (1996)

In 1994 and 1995, 233 different wild pigs were captured during population research at seven research sites focused primarily in the coastal regions of central and northern California. Mark-resight data and information on wild pig movements were used to assess wild pig population densities at those sites. Population densities ranged from 1.01 wild pigs/km² in Mendocino County in 1994 to 3.32 wild pigs/km² in Santa Clara County in 1995. Comparisons of population densities between years at three research sites suggested that wild pig populations increased in 1995 in response to favorable forage conditions after the wet fall and …

Animal Liberation And The Lessons Of Nature, Steve F. Sapontzis

Vertebrate Pest Conference Proceedings: 17th (1996)

Although they provide catchy labels, "animal liberation" and "animal rights" have occasioned considerable misunderstanding and much pointless debate. 1 want, here, to explicate what I believe is being sought for animals under these labels. This explication should help to undo some of the misunderstandings about liberating animals and extending moral rights to them. After this explication, I will turn to the issue of the way in which scientific knowledge of natural entities, processes, and organizations is and is not relevant to animal liberation.

PART I: WHAT ANIMAL LIBERATION IS ABOUT

One of these misunderstandings concerns the use of "animal" in …

Plant Secondary Chemicals As Non-Lethal Vertebrate Repellents, Richard W. Watkins, David P. Cowan, Elaine L. Gill

Vertebrate Pest Conference Proceedings: 17th (1996)

Few effective repellents are currently available for the non-lethal management of vertebrate pests. This is perhaps not surprising considering the ad hoc nature of past applications which assumed that the target pest species would have the same attraction/aversion preferences as man. A more rational approach is to identify compounds that have real biological significance for the pest species. Plants have evolved an array of defense chemicals (secondary plant compounds) that inhibit the feeding of vertebrate herbivores, because they are either innately aversive or they generate a conditioned aversion. These compounds are, therefore, ideally suited for use in the reduction of …

The Pocket Gopher As A Pest In Mexico, Desley Whisson, Beatriz Villa-C

Vertebrate Pest Conference Proceedings: 17th (1996)

Pocket gophers of the genus Orthogeomys and Pappogeomys are major pests in rangeland and agricultural areas throughout Mexico. Control relies on the indiscriminate use of fumigants and poison baits. These controls are applied in a haphazard manner; do not provide long-term benefits and the non-target hazards and public safety risks are perceived to be extremely high. Studies indicate that as a result of reinvasion of treated areas or territory expansion of animals surviving the control procedure, controls relying on removal of animals may be limited unless applied at frequent (every three months or less) intervals.

The Washington Adcp—A Private Collaborative Effort To Address Biological, Economical And Social Constraints To Reduce Wildlife Damage, Georg Ziegltrum, Dale L. Nolte

Vertebrate Pest Conference Proceedings: 17th (1996)

The Washington Animal Damage Control Program (WADCP) operates within the general structure of the Washington Forest Protection Association. The general goal of the WADCP is to resolve wildlife damage issues in an economically feasible and socially acceptable manner. The four components of the WADCP are program management and administration; support of individual member activities; research, monitoring, and surveys; and promotional and educational activities. An overview of each of these components is provided.

Public Health Hazards Associated With The Consumption Of Molluscan Shellfish In The State Of Rhode Island, Jeffrey F. Nussbaum

Marine Affairs Theses and Major Papers

The principal objective of this thesis was to descriptively and empirically assess shellfish consumption hazards in the state of Rhode Island and to evaluate the use of a shellfish hazard warning label to modify unsafe consumptive behaviors. To accomplish this task, three research problems were examined using a survey instrument. The survey instrument was a questionnaire which included both closed and open-ended questions. The survey questions were designed to identify four primary research variables; shellfish consumptive behaviors, consumer knowledge of shellfish hazards and related government advisories, perceptions of risk regarding personal shellfish consumption, and response to warning label information. The …

Trends In Abundance Of The Gulf Of Maine Population Of Harbor Porpoise, Paula Ann Olson

Marine Affairs Theses and Major Papers

Hundreds of harbor porpoise die each year as bycatch in the Gulf of Maine's sink gillnet fishery. In recent years, concern has arisen whether the Gulf of Maine population of harbor porpoise can sustain this level of bycatch. It is believed that the population may have declined, or is declining as a result of the incidental moralities. This paper reviews what is known about Gulf of Maine harbor porpoise demographics and how that information is incorporated into the management of the porpoise/fishery interaction. Included in this paper is a new study on the trend of relative abundance of harbor porpoise …

Protection Of Marine Fish Habitat: Towards An Integrated Approach To Management, Andrea L. Oliver

Marine Affairs Theses and Major Papers

This paper focuses on the problems associated with marine fish habitat protection and conservation at the federal level in the United States. The intent of the study is to provide a general overview of the issues, impacts, management problems and potential solutions. Federal statutory legislation that impacts marine fish habitat and agency authority derived from those legislative mandates are examined with a view toward promoting integrated management for marine biodiversity and overall ecosystem health. The concepts of coordinated and integrated approaches to management are discussed in the context of the present management regimes. Given the fragmented authority over the policy …