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Development And Validation Of Gas Chromatographic Methods For Free Fatty Acid Determination In Dairy Products, David Mannion Jan 2016

Development And Validation Of Gas Chromatographic Methods For Free Fatty Acid Determination In Dairy Products, David Mannion

Theses

Quantification of free fatty acids (FFA) in dairy products by gas chromatography flame ion'^ation detection is an important requirement for qua'ity, research, nutritional, authenticity and legislative purposes. Common procedures are typically variants of either the fatty acid methyl esterification (FAME) or direct on-column injection methods developed more than two decades ago. This study involved the development of an alternative novel derivatization method using butylation, incorporating automation, reducing solvent usage that is applicable for a wide range of dairy products. The accuracy, precision, linearity, limits of detection and limits of quantification of all three methods were assessed for the quantification of …


Evaluation Of Bobcat (Lynx Rufus) Survival, Harvest, And Population Size In The West-Central Region Of South Dakota, Brandon M. Tycz Jan 2016

Evaluation Of Bobcat (Lynx Rufus) Survival, Harvest, And Population Size In The West-Central Region Of South Dakota, Brandon M. Tycz

Electronic Theses and Dissertations

Recent concern regarding bobcat (Lynx rufus) population status has prompted researchers and managers to gather additional information about bobcats in South Dakota. From 2012–2015, we assessed population dynamics of bobcats occupying the west-central region of South Dakota. Our objectives were to: 1) estimate annual survival rates; 2) determine cause-specific mortality; 3) estimate a population size for the western prairie region of South Dakota; 4) estimate home range size of individually marked bobcats; 5) evaluate reproductive status; and 6) build a population model. We captured and radio-collared 51 (24 male, 27 female) bobcats with VHF collars. Annual survival was 65.1% (95% …


Evaluation Of Bison (Bison Bison) Ecology At The Olson’S Bison Conservation Ranches, Pine River Ranch, Manitoba, Canada, Joshua L. Leonard Jan 2016

Evaluation Of Bison (Bison Bison) Ecology At The Olson’S Bison Conservation Ranches, Pine River Ranch, Manitoba, Canada, Joshua L. Leonard

Electronic Theses and Dissertations

Bison (Bison bison), the largest mammalian species in North America, historically numbering in the tens of millions (Roe 1970, McHugh 1972, Dary 1989, Shaw 1995), was nearly extirpated at the turn of the 19th century (Knapp et al. 1999; Gates et al. 2010). At the time, estimates of less than 1,000 bison remaining in North America are widely accepted (Hornaday 1889; Seton 1927; Gates et al. 2010). The decline of bison includes factors such as disease (Flores 1991; Isenberg 2000), sport hunting (Danz 1997; Dary 1989; Hewitt 1919; Isenberg 2000; McHugh 1972), and unofficially funded commercial hunting by the U.S. …


Effect Of Oil And Natural Gas Development On Survival And Health Of White-Tailed Deer In The Western Dakotas, Katherine L. Moratz Jan 2016

Effect Of Oil And Natural Gas Development On Survival And Health Of White-Tailed Deer In The Western Dakotas, Katherine L. Moratz

Electronic Theses and Dissertations

Oil and gas development in North Dakota has resulted in the need for information regarding how increased activity has affected white-tailed deer (Odocoileus virginianus) populations. We evaluated white-tailed deer ecology in response to energy development and hypothesized that oil and gas development would negatively affect adult and neonate white-tailed deer due to increased vehicle traffic and human-related effects. We captured and radio-collared adult female and neonate white-tailed deer across three study areas: Dunn County, North Dakota, an area influenced by energy development, and Grant County, North Dakota, and Perkins County, South Dakota, areas not impacted by energy development at this …


The Effect Of Copper And Zinc Source On Pre-Weaning Performance Of Cows, Health And Performance Of Suckling Calves, And Post-Weaning Feedlot Performance, Carcass Composition, And Meat Quality Of Calves, Jesse Fulton Jan 2016

The Effect Of Copper And Zinc Source On Pre-Weaning Performance Of Cows, Health And Performance Of Suckling Calves, And Post-Weaning Feedlot Performance, Carcass Composition, And Meat Quality Of Calves, Jesse Fulton

Electronic Theses and Dissertations

During gestation an elevated level of nutrient intake is required by the cow in order to meet the requirements of the maturing fetus and if these requirements are not met, changes in fetal development can occur. However, little is understood about the effects of gestational manipulation of specific nutrients, such as minerals, on progeny growth, performance and carcass traits. Previous research supports the hypothesis that minerals provided in an organic form should be more soluble and readily available to the body for absorption. Therefore the objectives of this study were to determine if source of gestational and post-natal Cu and …


Manufacture, Concentration, And Functionality Of Micellar Casein Concentrate, Dustin Grossbier Jan 2016

Manufacture, Concentration, And Functionality Of Micellar Casein Concentrate, Dustin Grossbier

Electronic Theses and Dissertations

This research has been structured into 3 major parts. The first part evaluates a centrifugal evaporator to concentrate micellar casein concentrate (MCC). The second part evaluates a wiped film evaporator (WFE) to concentrate MCC. The third part evaluates heat stability of the high concentration MCC produced by WFE. In part 1, the primary objective was to achieve >25% total solids (TS) without a loss of water removal rate associated with high solids materials. This is of importance, industrially, as this could reduce processing costs or eliminate drying all together. The major finding was that it did not offer any benefits …


Three Overlooked Issues: Water Quality, Forage Quality, And Forage Blends Impacting The Feed Efficiency Of Dairy Cows, Ishwary Prasad Acharya Jan 2016

Three Overlooked Issues: Water Quality, Forage Quality, And Forage Blends Impacting The Feed Efficiency Of Dairy Cows, Ishwary Prasad Acharya

Electronic Theses and Dissertations

The overall objectives of the six studies were to increase the feed efficiency in lactating dairy cows. The first study evaluated the efficacy of different water sources and treatment systems on ruminal parameters using an in vitro gas production system. Outcomes of the study suggested that the source and nutrient quality of water can affect rate of ruminal fermentation. Thus, use of real farm water being offered to the cow to conduct in vitro gas production measurements may accurately predict the ruminal fermentation, digestibility and total gas production. The second study evaluated two recently developed leafy-floury corn silage hybrids against …


Survival, Abundance, And Relative Predation Of Wild Rainbow Trout In The Deerfield Reservoir System, South Dakota, Jeremy L. Kientz Jan 2016

Survival, Abundance, And Relative Predation Of Wild Rainbow Trout In The Deerfield Reservoir System, South Dakota, Jeremy L. Kientz

Electronic Theses and Dissertations

Rainbow Trout Oncorhynchus mykiss are routinely stocked in Black Hills streams and reservoirs to enhance angling opportunities for the public, however in most cases, hatchery-reared Rainbow Trout do not successfully recruit to establish natural populations. One exception is the Deerfield Reservoir system, where it is estimated that up to 25% of the Rainbow Trout population consists of naturally produced, wild Rainbow Trout. While recruitment of wild Rainbow Trout to the Deerfield Reservoir fishery does occur, annual stockings of 12,000 hatchery Rainbow Trout have continued. In recent years, adipose fin clips were used to identify hatchery Rainbow Trout stocked into Deerfield …


Evaluation Of The Hypothalamic Kisspeptin System Throughout The Estrous Cycle And During The Attainment Of Puberty In Gilts, Eric Steven Jolitz Jan 2016

Evaluation Of The Hypothalamic Kisspeptin System Throughout The Estrous Cycle And During The Attainment Of Puberty In Gilts, Eric Steven Jolitz

Electronic Theses and Dissertations

Kisspeptin has been demonstrated to affect reproductive cyclicity and the attainment of puberty in multiple species, presumably through its actions on GnRH and LH, as demonstrated in other species. Kisspeptin administration causes increased plasma concentrations of LH in pigs, sheep, and rats. Two experiments were conducted to evaluate 1) the hypothalamic kisspeptin system throughout various days of the estrous cycle and 2) the hypothalamic kisspeptin system during the peripubertal period in gilts. In experiment 1, 40 crossbred gilts (191 d, 121 kg) were administered an intramuscular injection of PG600. Twelve days after the administration of PG600, gilts were fed 15 …


An Evaluation Of Feeding A Blend Of Essential Oils And Cobalt Lactate To Lactating Dairy Cows, Olivia Jayne Kuester Jan 2016

An Evaluation Of Feeding A Blend Of Essential Oils And Cobalt Lactate To Lactating Dairy Cows, Olivia Jayne Kuester

Electronic Theses and Dissertations

The objective of this study was to determine if the inclusion of a blend of essential oils and cobalt lactate (EOC) in the diet of lactating dairy cows could result in improved nutrient utilization and lactational performance in addition to assessing the application of EOC to control feed mycotoxins and spoilage. The trial was conducted in two experimental periods (Phase 1 (P1) and Phase 2 (P2)) on a commercial dairy in southwest Minnesota equipped with two robotic milking units. Phases 1 and 2 were cross over design trials with two pens. Cows were housed in two freestall pens (57 ± …


Raptors In Temperate Grasslands: Ecology Of Ferruginous Hawk, Golden Eagle, And Northern Harrier In The Northern Great Plains, Shubham Datta Jan 2016

Raptors In Temperate Grasslands: Ecology Of Ferruginous Hawk, Golden Eagle, And Northern Harrier In The Northern Great Plains, Shubham Datta

Electronic Theses and Dissertations

Ferruginous hawks (Buteo regalis) are a grassland and shrubland obligate nesting raptor and prefer lightly grazed pasture or idle areas for nesting. Their population reportedly declines in number if more than 30% of an area is cultivated and they rarely nest in areas dominated by croplands. Golden eagles (Aquila chrysaetos) are long-lived raptors with high nest-site fidelity and relatively low reproductive success. Population trends of golden eagles in western United States are unclear although long-term monitoring of populations shows declines in occupancy and breeding performance. Northern harriers (Circus cyaneus) prefer relatively open grasslands and …


Improving Feed Efficiency Through Forage Strategies For Increasing Dairy Profitability And Sustainability, Jon Patrick Pretz Jan 2016

Improving Feed Efficiency Through Forage Strategies For Increasing Dairy Profitability And Sustainability, Jon Patrick Pretz

Electronic Theses and Dissertations

Three studies were conducted to determine production parameters and study specific hypothesis in regard to improving feed efficiency through various forage utilization strategies with or without the inclusion of various supplemented products. The first study evaluated the supplementation of a cobalt-lactate product and its effects on fiber digestibility and milk production parameters when fed to cows consuming a 70% forage diet. Treatments included: 1) CONTROL diet containing 12.5 mg/cow/d of cobalt (carbonate carbonate) and 2) TEST diet being the same basal diet but including an additional 50 mg/cow/d of cobalt via a 1% Co-lactate product (Co-Max®). In a feeding trial …


Geographic Range Of The Western Harvest Mouse (Reithrodontomys Megalotis) In Arkansas, G. R. Graves Jan 2016

Geographic Range Of The Western Harvest Mouse (Reithrodontomys Megalotis) In Arkansas, G. R. Graves

Journal of the Arkansas Academy of Science

No abstract provided.


Unusual Concentration Of Summer Tanagers (Piranga Rubra) In Northwestern Arkansas During Winter 2015-2016, K. G. Smith, J. C. Neal, M. Mlodinow Jan 2016

Unusual Concentration Of Summer Tanagers (Piranga Rubra) In Northwestern Arkansas During Winter 2015-2016, K. G. Smith, J. C. Neal, M. Mlodinow

Journal of the Arkansas Academy of Science

No abstract provided.


Spatial And Temporal Patterns In Irruptive Fringillid Movements, Paul J. Dougherty Jan 2016

Spatial And Temporal Patterns In Irruptive Fringillid Movements, Paul J. Dougherty

Honors Theses

Historic Christmas Bird Count and eBird data indicate that during winter invasions, North American irruptive fringillid species occur in relatively even numbers across latitudes east of the Rocky Mountains. Most boreal breeding species show large southward invasions every other winter, with this pattern typically being strongest across the northern and mid United States. eBird data confirms that many northern fringillids irrupt synchronously, and both datasets also show positive correlations between the winter movements of southern breeding species in many areas. Winter abundance records show negative correlations between northern breeding species and southern breeding species in nearly all monitored areas of …


The Impact Of Endocrine-Disrupting Chemicals On Wildlife Conservation, Eda Reed Jan 2016

The Impact Of Endocrine-Disrupting Chemicals On Wildlife Conservation, Eda Reed

Honors Theses

Endocrine-disrupting chemicals (EDCs), such as those from plastics and pesticides, have been hypothesized to affect wildlife populations. According to the U.S. National Institute of Environmental Health Sciences, EDCs ‘are chemicals that may interfere with the body’s endocrine system and produce adverse developmental, reproductive, neurological, and immune effects in both humans and wildlife.’ A growing number of studies suggest wildlife are being exposed to EDCs, but how EDCs affect wildlife health and wildlife conservation is less well-understood. Through scientific literature analysis and a survey of wildlife conservationists and practitioners, this study aims to determine the current state of knowledge and data …


Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen Jan 2016

Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare PCR tests for the detection of Mycoplasma agalactiae in sheep and goats. Samples obtained from sheep and goats in Turkey were tested to determine the presence of M. agalactiae. Mycoplasma culture and two direct PCR methods based on the uvrC gene and polC gene were compared with PCR of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (PCR/DGGE) on DNA extracted from clinical samples (following culture enrichment). A total of 234 samples were examined: 9.4% and 13.2% were positive for M. agalactiae based on the uvrC gene and polC gene …


Genetic Analysis Of Avian Paramyxovirus Type I Strains Isolatedfrom Backyard Poultry In Iran, Fereshteh Sabouri, Mehdi Vasfi Marandi, Vahid Karimi, Mohammad Malekan, Mohsen Bashashati Jan 2016

Genetic Analysis Of Avian Paramyxovirus Type I Strains Isolatedfrom Backyard Poultry In Iran, Fereshteh Sabouri, Mehdi Vasfi Marandi, Vahid Karimi, Mohammad Malekan, Mohsen Bashashati

Turkish Journal of Veterinary & Animal Sciences

Newcastle disease (ND) is one of the most serious diseases amongst many species of birds and causes huge economic losses in the poultry sector. Backyard poultry are the main source of food in rural areas of many developing countries and play a crucial role in the epidemiology of ND. The sequence of the F protein cleavage site is the major virulence determinant of Newcastle disease virus (NDV). The aim of this study was to understand the molecular characterization of F proteins of Iranian backyard poultry in NDVs. The complete open reading frame sequences of the F gene of six isolates …


Assessment Of The Efficacy Of The Hose-Bridge As A Road-Crossing Structure For Dekay's Brownsnake (Storeria Dekayi), Sarah Elizabeth Manka Jan 2016

Assessment Of The Efficacy Of The Hose-Bridge As A Road-Crossing Structure For Dekay's Brownsnake (Storeria Dekayi), Sarah Elizabeth Manka

Masters Theses

Various structures have been integrated into roadways to ensure the road does not impede wildlife movements between adjacent habitats. Where traffic volume is low, the costs of installing and maintaining such structures is typically prohibitive. I tested the efficacy of a hose-bridge deployed temporarily on a State Park road in Illinois that allowed the passage of cars over it and small wildlife species to cross through it. I predicted that, during the 4.5 -week period of peak migratory movement, road-based mortality of Dekay's Brownsnakes (Storeria dekayi) would be less at the site having the hose-bridge, when compared to a similar …


Effects Of Habitat Alteration On Ecomorphology Of Fish Communities In A Restored Stream, Carl Anthony Favata Jan 2016

Effects Of Habitat Alteration On Ecomorphology Of Fish Communities In A Restored Stream, Carl Anthony Favata

Masters Theses

Decades of anthropogenic pressure have devastated lotic ecosystems across the riverscapes of North America, resulting in degradation of critical habitat and contributing to sharp declines in biotic integrity. In response, local stream restoration projects have increased in frequency. However, little effort has been allocated to monitoring and project success has been limited. In addition, restoration projects typically focus on ecological effects above the population-level, while relationships with physiological processes are seldom assessed. Lessons from the long-term restoration and ecological monitoring of Kickapoo Creek highlight some of the complex dynamics driving reach-scale restoration projects. Following instream restoration, I predicted that alterations …


Sampling Efficiency, Population Characteristics, And Potential Impacts Of Harvest Regulations On Three Riverine Species Of Catfish, Zachary Adam Mitchell Jan 2016

Sampling Efficiency, Population Characteristics, And Potential Impacts Of Harvest Regulations On Three Riverine Species Of Catfish, Zachary Adam Mitchell

Masters Theses

No abstract provided.


Genetic Structure And Diversity Of Channel Catfish (Ictalurus Punctatus) In Three Large Midwestern Rivers, Vaclav Alexei Sotola Jan 2016

Genetic Structure And Diversity Of Channel Catfish (Ictalurus Punctatus) In Three Large Midwestern Rivers, Vaclav Alexei Sotola

Masters Theses

Little information is available regarding the genetic structure and diversity of sportfishes in large rivers, which can be vital to continuing the sustainable exploitation of these fisheries. Additionally, impoundments often have vast impacts on riverine systems. These impacts include altering the flow regime, changing the nutrient load, and preventing migratory fish from reaching their spawning grounds. If fishes are prevented from reaching their spawning grounds, their genetic diversity and structuring could begin to change relative to historic measures, potentially decreasing their fitness and ability to survive. I screened microsatellite loci to assess the genetic population structure and diversity of channel …


Effects Of Visitors And Enrichments On Behavior Of Captive Red Wolves’ (Canis Rufus) At The Great Plains Zoo, Sioux Falls, South Dakota, Kylee S. Shotkoski Jan 2016

Effects Of Visitors And Enrichments On Behavior Of Captive Red Wolves’ (Canis Rufus) At The Great Plains Zoo, Sioux Falls, South Dakota, Kylee S. Shotkoski

Electronic Theses and Dissertations

Red wolves (Canis rufus) are the first animals to maintain a wild population from captive, released individuals. A captive breeding program for red wolves was started before complete extirpation, and 4 breeding pairs were released in Alligator River National Wildlife Refuge (North Carolina) in 1987 and a small wild population still exists there. Currently, there are several captive breeding facilities for red wolves within the Species Survival Plan (SSP) program. The Great Plains Zoo in Sioux Falls, South Dakota participates in the SSP program. My study was initiated to create a natural history background and evaluate interaction between …


A Physiological Assessment Of Wetland Habitats For Spring-Migrating Ducks In The Agricultural Landscapes Of The Southern Prairie Pothole Region, Adam K. Janke Jan 2016

A Physiological Assessment Of Wetland Habitats For Spring-Migrating Ducks In The Agricultural Landscapes Of The Southern Prairie Pothole Region, Adam K. Janke

Electronic Theses and Dissertations

The conversion of grassland and wetland ecosystems in the Prairie Pothole Region (PPR) has been a pervasive challenge for conservationists dating back to the early 1900s. The legacy of ever-increasing agricultural intensity in the southern portions of the PPR, including eastern South Dakota, has left many wetland ecosystems in a matrix of intensive agricultural production. With little surrounding nesting cover, these wetlands are thought to have limited potential for waterfowl reproduction but may still play an important role facilitating migration of waterfowl en route to northern breeding areas during spring. My research sought to understand the contributions of wetlands in …


Effect Of Dietary Supplementation With Different Levels Of Inulin-Typefructans On Renal Expression Of Aquaporin 2 Of Growing Piglets, Katarzyna Michalek, Marta Grabowska, Mariusz Skowronski, Adam Lepczynski, Agnieszka Herosimczyk, Maria Laszczynska Jan 2016

Effect Of Dietary Supplementation With Different Levels Of Inulin-Typefructans On Renal Expression Of Aquaporin 2 Of Growing Piglets, Katarzyna Michalek, Marta Grabowska, Mariusz Skowronski, Adam Lepczynski, Agnieszka Herosimczyk, Maria Laszczynska

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to identify and investigate the expression of aquaporin 2 (AQP2) in the renal tubules of growing piglets fed with different levels of inulin-type fructans. The experiment was performed on five groups of PIC x Penarlan P76 crossbred piglets fed with the following diets: standard diet (control group) and standard diet supplemented with 1% (group I), 2% (group II), and 3% (group III) of water extract of chicory root inulin-type fructans. The animals from the group IV were fed with standard diet supplemented with 4% dried chicory roots. To analyze immunolocalization and expression of AQP2 …


Effects Of Bran Treatment On Rheology And Sensory Quality Of Whole Wheat Flat Bread, Jigyasha Mishra Jan 2016

Effects Of Bran Treatment On Rheology And Sensory Quality Of Whole Wheat Flat Bread, Jigyasha Mishra

Electronic Theses and Dissertations

The purpose of this research was to study the differences in the quality of 100% whole wheat flour (WWF) when the bran fraction was subjected to mild solvent treatment before reconstitution with the endosperm. The bran treatments included water washing and washing with ethanol solutions (50% and 100%). WWF prepared with untreated bran served as the control. Effects of these treatments on particle size, chemical composition, color, dough rheology, product formulation, and sensory characteristics of 100% whole wheat flour were studied. Grains of five HRSW cultivars (Advance, Prevail, Select, Brick and Forefront) were milled using a Quadrumat Senior mill. Gluten …


Current Status Of Falcon Populations In Saudi Arabia, Albara M. Binothman Jan 2016

Current Status Of Falcon Populations In Saudi Arabia, Albara M. Binothman

Electronic Theses and Dissertations

Falcons (Falco spp.) are widely used for falconry in the countries of the Middle East. During the 2015 breeding season, we surveyed historic and active nest sites of Barbary (Falco pelegrinoides pelegrinoides) and Lanner (F. biarmicus) Falcons in Saudi Arabia. Field and questionnaire surveys were conducted and personal contact with falconers was made to document the current distribution and price changes for Lanner Falcons, Barbary Falcons, Saker Falcons (F. cherrug), Peregrine Falcons (F. peregrinus), Gyrfalcons (F. rusticolus), and Hybrid Falcons in Saudi Arabia. We categorized our survey into three geographic groups; southwest (A), northwest (B), and central (C) regions of …


Growth Performance, Nutrient Utilization, And Metabolic Profile Of Dairy Heifers Fed Diets High In Distillers Grains With Different Forage To Concentrate Ratios, Angela Kristia Manthey Jan 2016

Growth Performance, Nutrient Utilization, And Metabolic Profile Of Dairy Heifers Fed Diets High In Distillers Grains With Different Forage To Concentrate Ratios, Angela Kristia Manthey

Electronic Theses and Dissertations

Two studies were conducted to evaluate the effect of limit-feeding heifers distillers grains with solubles (DDGS) with varying forage to concentrate ratios. The effects on growth, feed efficiency, rumen fermentation, nutrient digestibility, metabolic profile and onset of puberty, as well as post trial performance were investigated. First, a 16-wk feeding trial was conducted using 48 Holstein heifers to evaluate effects of dietary treatment on dry matter intake (DMI), average daily gain (ADG), growth performance, rumen fermentation, and nutrient digestibility. Treatments were 1) 30% DDGS, with the diet fed at 2.65% of body weight (BW) (30DG), 2) 40% DDGS, with the …


Response Of Soil And Water Quality To Winter Manure Application From Small Agricultural Watersheds In South Dakota, Shikha Singh Jan 2016

Response Of Soil And Water Quality To Winter Manure Application From Small Agricultural Watersheds In South Dakota, Shikha Singh

Electronic Theses and Dissertations

The extreme winter conditions prevailing in the state of South Dakota make it difficult for the livestock producers to manage the manure generated at the farm. The South Dakota Department of Environmental and Natural Resources does not recommend manure application in the state during the winter months when the ground is frozen. Thus, producers are left with the options such as storing the manure over a longer period until summer or spreading on snow or frozen ground. Storing manure for longer duration leads to increased risks of concentrated spills into the streams. Thus, it is important to develop management strategies …


An Evaluation Of Rumen Modifiers For Lactational Performance And Nutrient Digestibility By Cows, Prakash Poudel Jan 2016

An Evaluation Of Rumen Modifiers For Lactational Performance And Nutrient Digestibility By Cows, Prakash Poudel

Electronic Theses and Dissertations

Three studies were conducted to evaluate a commercial blend of essential oils, active dried yeast product and cobalt lactate on production performance, rumen fermentation and nutrient digestibility by cows by using in vivo and in vitro methods. The first study evaluated the lactational performance and nutrient digestibility of lactating Holstein dairy cows fed two commercial supplements: 1) a propriety blend of essential oils and Cobalt lactate (EOC) (Stay Strong, Ralco, Inc., Marshall, MN) and 2) active dried yeast (ADY) (Omnigen AF, Prince Agri Products, Inc., Quincy, IL). The 12-week experiment demonstrated no differences in milk production, milk composition, and feed …