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Summer-Long Grazing Of High Vs. Low Endophyte (Neotyphodium Coenophialum)-Infected Tall Fescue By Growing Beef Steers Results In Distinct Temporal Blood Analyte Response Patterns, With Poor Correlation To Serum Prolactin Levels, Joshua J. Jackson, Merlin D. Lindemann, James A. Boling, James C. Matthews

Animal and Food Sciences Faculty Publications

Previously, we reported the effects of fescue toxicosis on developing Angus-cross steer growth, carcass, hepatic mRNA, and protein expression profiles of selected serum proteins, and blood clinical and chemical profiles, after summer-long grazing (85 days) of high endophyte (HE)- vs. low endophyte (LE)-infected fescue pastures. We now report the temporal development of acute, intermediate, and chronic responses of biochemical and clinical blood analytes determined at specified time intervals (period 1, day 0–36; period 2, day 37–58; and period 3, day 59–85). Throughout the trial, the alkaloid concentrations of the HE forage was consistently 19–25 times greater (P ≤ 0.002) …

A Novel Thermal Gradient Design For Small-Bodied Ectotherms, Michael M. Webber, Robert W. Bryson Jr.

Euscorpius

For ectothermic organisms, environmental temperatures can influence a variety of life history traits. Experimental thermal gradients created in the laboratory are useful tools to examine the thermoregulatory behaviors of these organisms. Here, we provide details on the construction of a novel, cost-effective thermal gradient to study thermoregulatory behaviors in small-bodied nocturnal ectotherms.

Longevity Of Mineral Supplements Within The Soil And Associated Use By White-Tailed Deer, Brian C. Peterson, Keith D. Koupal, Andrew K. Schissel, Cody M. Siegel

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Humans have baited wildlife such as white-tailed deer (Odocoileus virginianus) for generations with the primary purpose of increasing hunting harvest success. Baiting regulation changes are often considered by state management agencies as they pertain to hunting opportunity, fair chase, and disease risk. Cervids require a variety of minerals to supplement biological processes, especially sodium (Na), calcium (Ca), and phosphorus (P). We developed artificial mineral supplement sites set in front of trail cameras to monitor deer use. Pooled soil samples were collected at mineral sites and compared to the surrounding area to determine the longevity of elevated minerals levels …

Genomic Legacy Of The African Cheetah, Acinonyx Jubatus, Pavel Dobrynin, Shiping Liu, Gaik Tamazian, Zijun Xiong, Andrey A. Yurchenko, Ksenia Krasheninnikova, Sergey Kliver, A. Schmidt-Kunzel, Klaus-Peter Koepfli, Warren E. Johnson, Lukas F. K. Kuderna, Raquel Garcia-Perez, Marc De Manuel, Ricardo Godinez, Aleksey Komissarov, Alexey Makunin, Vladimir Brukhin, Weilin Qiu, Long Zhou, Fang Li, Jian Yi, Carlos A. Driscoll, Agostinho Antunes, T. K. Oleksyk, Eduardo Eizirik, Polina Perelman, Melody E. Roelke, David E. Wildt, Mark Diekhans, Tomas Marques-Bonet, Laurie Marker, Jong Bhak, Jun Wang, Guojie Zhang, Stephen J. O'Brien

Biology Faculty Articles

Background

Patterns of genetic and genomic variance are informative in inferring population history for human, model species and endangered populations.

Results

Here the genome sequence of wild-born African cheetahs reveals extreme genomic depletion in SNV incidence, SNV density, SNVs of coding genes, MHC class I and II genes, and mitochondrial DNA SNVs. Cheetah genomes are on average 95 % homozygous compared to the genomes of the outbred domestic cat (24.08 % homozygous), Virunga Mountain Gorilla (78.12 %), inbred Abyssinian cat (62.63 %), Tasmanian devil, domestic dog and other mammalian species. Demographic estimators impute two ancestral population bottlenecks: one >100,000 years …

Production And Economic Efficiencies Of Intensified Cow–Calf Production Management Systems, Jason M. Warner

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of four experiments conducted was to evaluate production and economic efficiencies of intensified cow-calf production management systems. The first experiment tested the effect of calf age at weaning on cow and calf performance and feed utilization at 2 locations. Body weight change from early to conventional weaning time was greater for early-weaned cows. Cow BCS and conception rates were not impacted by weaning. Calf BW at conventional weaning time was greater for conventionally-weaned calves than early-weaned calves at ARDC, but greater for early-weaned than conventionally-weaned calves at PHREC. Calf ADG per unit of total feed energy intake was …

Avs Corner, December 2015, Casey Wheeler, Emily Cook, Alexandra Johnson, Jamison Lynch, Kim Hill, Hayley Boyd, April Jeffcoat, Johanna Johnson

AVS Corner

No abstract provided.

Efficiency Of Energy Utilization And Fecal Mineral Excretion In Holstein And Jersey Dairy Cows, Gabriel Garcia Gomez

Department of Animal Science: Dissertations, Theses, and Student Research

Eight multiparous Holstein and 8 Jersey dairy cows were used to complete energy balance trials designed to determine the effect of breed on the production and energy efficiency of milk, and energy partitioning. A repeated switchback design was used to compare the effect of cow breed. Diets consisted of 24.5% corn silage, 18.4% alfalfa hay, 6.9% grass hay, with either 22.9% rolled corn and 14.8% soybean meal (CON) or 8.95% rolled corn and 28.8% reduced fat distillers grains (RFDDGS) DM-basis. Diets were offered ad libitum for a 28 d adaptation period and 95% ad libitum for a 4 d collection …

Feeding A Brown Midrib Corn Silage-Based Diet To Growing Beef Steers Improves Growth Performance And Economic Returns, C. S. Saunders, S. Y. Yang, J.-S. Eun, D. M. Feuz, D. R. Zobell

Animal, Dairy, and Veterinary Science Faculty Publications

A feedlot experiment was performed to determine growth performance, ruminal fermentation characteristics, and economic returns for growing beef steers when fed a brown midrib corn silage-based total mixed ration (BMRT) compared with a conventional corn silage-based total mixed ration (CCST). Twenty-four Angus crossbred steers (initial body weight=258±23.2 kg) in individual pens were used in a completely randomized design (n=12). Intake of dry matter was not different between the treatments. Steers fed the BMRT tended to have greater average daily gain (1.54 vs. 1.42 kg d−1; P=0.09) and gain-to-feed ratio (0.165 vs. 0.146; P=0.07) compared with those fed the CCST. Feeding …

Leptin Regulates The Expression Of Autophagy-Related Genes In Chickens, Peter Olawale Ishola

Graduate Theses and Dissertations

Autophagy or cellular self-digestion, a lysosomal degradation pathway that is conserved from yeast to human, plays a key role in recycling cellular constituents, including damaged organelles. It also plays a pivotal role in the adaptation of cells to a plethora of distinct stressors including starvation. Autophagy has been extensively studied in mammals and yeast, but little is known in avian species. Thus, the major objective of the present study was to determine the effects of leptin on autophagy-related genes in chicken hypothalamus, muscle and liver. Leptin is an adipocytokine that is mostly produced by white adipose cells in mammals (as …

Broiler Breeder Mating Behavior And The Effects Of Pre-Incubation Embryo Integrity Modifications On Broiler Performance, Emily Kathryn Lhamon

Graduate Theses and Dissertations

Modern broiler breeders have been observed displaying significantly less mating behaviors than their ancestral fowl. Effective mating behavior translates into fertile hatching eggs that ultimately become broilers processed for market. The poultry industry has made significant changes in genetics, environment, housing, and diets of commercial poultry. However, there are some concessions made in genetic selection and streamlined management to produce larger, fast-growing, efficient birds. Aggression in broiler breeder males is believed to be a sign of high fertility. However, highly aggressive males pose a threat to the welfare of their flock mates, and may also impede frequency of mating and …

Responses Of Two Strains Of Broilers To Diets Varying In Essential Amino Acid Density And Energy Levels, Tyler James Clark

Graduate Theses and Dissertations

The responses of two different commercial strains of broilers to diets varying in overall amino acid levels and metabolizable energy levels were observed. Amino acid levels used were 80%, 90%, 100%, 110%, 120%, and 130% of Ross recommended minimum digestable levels of essential amino acids. Energy levels used were either below or above Ross recommended metabolizable energy levels. Diets were fed for 56 days. The two strains were a fast growing broiler strain and a slower growing broiler strain. A selection of birds from each pen was processed at 35, 43, and 56 days of age. Average body weights, cumulative …

Impact Of Urban Factors And Invasive Species On White-Footed Mouse (Peromyscus Leucopus) Habitat Use And Foraging Behavior In An Urban Forest Park., William Persons

Electronic Theses and Dissertations

White-footed mice (Peromyscus leucopus) select for areas of greater canopy cover at the macro scale, and for areas with more CWD at the micro-scale. White-footed mice potentially avoid habitats with higher invasive species richness. White-footed mice preferentially foraged under the honeysuckle canopy in response to changes in temperature and humidity. This study suggests that the interaction between P. leucopus and ground layer invasive species is complex, and that the effect of moonlight may be diminished in this urban park. Urban ecosystems demonstrate high levels of anthropogenic land-use change, modification of abiotic inputs, and altered disturbance regimes. These changes result …

Influence Of Habitat And Intrinsic Characteristics On Survival Of Neonatal Pronghorn, Christopher N. Jacques, Jonathan A. Jenks, Troy W. Grovenburg, Robert Wayne Klaver

Natural Resource Management Faculty Publications

Increased understanding of the influence of habitat (e.g., composition, patch size) and intrinsic (e.g., age, birth mass) factors on survival of neonatal pronghorn (Antilocapra americana) is a prerequisite to successful management programs, particularly as they relate to population dynamics and the role of population models in adaptive species management. Nevertheless, few studies have presented empirical data quantifying the influence of habitat variables on survival of neonatal pronghorn. During 2002–2005, we captured and radiocollared 116 neonates across two sites in western South Dakota. We documented 31 deaths during our study, of which coyote (Canis latrans) predation (n = 15) was the …

Mountain Big Sagebrush (Artemisia Tridentata Ssp Vaseyana) Seed Production, Melissa L. Landeen

Theses and Dissertations

Big sagebrush (Artemisia tridentata Nutt.) is the most widespread and common shrub in the sagebrush biome of western North America. Of the three most common subspecies of big sagebrush (Artemisia tridentata), mountain big sagebrush (ssp. vaseyana; MBS) is the most resilient to disturbance, but still requires favorable climactic conditions and a viable post-fire seedbank for successful unassisted recovery. This study was designed to assess MBS seed production throughout post-fire recovery. We performed 2 pilot studies to develop methods for estimating seed production and plant age. The results of the pilot studies and a space-for-time substitution strategy were used to measure …

Gut Microbiota Of Migratory Passerines At Stopover, William Bagley Lewis

Master's Theses

Although the gut microbiota provides many beneficial functions to animal hosts, relatively little is known about the gut microbiota of passerines. It is likely that gut microbes are especially important during the migratory phase; however gut atrophy experienced during prolonged migratory flight may cause disruptions of the stable microbiota. Fecal samples were collected from several species of passerine after crossing the Gulf of Mexico during spring migration and before crossing during fall, and microbiota communities were analyzed using next-generation sequencing. Despite showing large inter-individual variation, a core microbiota composed largely of Firmicutes and Proteobacteria was identified in all birds. Microbiota …

Maternal Effects And Offspring Behavior: Potential Contributors To The Lack Of Recruitment In Mississippi Gopher Tortoises (Gopherus Polyphemus), Aaron L. Holbrook

Master's Theses

Federally threatened in Mississippi, gopher tortoise (Gopherus polyphemus) populations within the state have strongly variable recruitment and are generally in decline. Hatching success is significantly lower in Mississippi than in any other part of the species’ range , and most hatchlings die within the first year. There are few refuges where survival and hatching success is high. Here I compare two populations that differ in recruitment and offspring survival for differences in corticosterone. Corticosterone is a hormone that influences both energy availability and is released in elevated levels during stressful events, like living in poor quality habitat. Prolonged corticosterone elevation …

Evaluation Of Blue Tilapia (Oreochromis Aureus) For Duckweed (Lemna Minor) Control In South Carolina's Private Waters, William Heaton

All Dissertations

ABSTRACT We investigated the potential of Blue Tilapia (Oreochromis aureus) as a means for biological control of duckweed in agricultural impoundments, and assessed the potential for naturalization of this non-native fish in coastal regions of South Carolina. Additionally, we studied growth characteristics and nutrient accumulation abilities of duckweed (Lemna minor) growing in agricultural impoundments in the piedmont and coastal plain. Observations of tank feeding trials indicated juvenile Blue Tilapia consumed 36 – 86% of their body weight in fresh duckweed every 24 hours. Tilapia were stocked in agricultural impoundments in the piedmont and coastal plain during the 2012 and 2013 …

Digestibility Of Two Complete Pelleted Diets By The Horse (Equus Caballus) As A Model Animal For Nondomestic Hindgut Fermenters, Emily M. Schwartz

Master's Theses

Estimating nutrient and energy requirements of exotic animals is a necessary component of nutrition management in zoos and other wildlife facilities. In the absence of species-specific data, domestic animal models are often referenced. Herbivorous hindgut fermenters, such as horses, zebra, and rhinoceros, rely on microbial fermentation in the cecum and colon to utilize dietary structural carbohydrates. The study objective was to measure the digestible energy of two (LOW, HIGH) complete pelleted diets by the horse as a model for nondomestic hindgut fermenters. Seven, individually housed, adult Quarter Horse (Equus caballus) geldings were assigned to one of two diets as 100% …

Non-Suitable Habitat A Cause For Declining Bobolink Populations In Northern Utah, Bethany Q. Unger

Undergraduate Honors Capstone Projects

Bobolink, Dolichonyx oryxivories, populations are declining in Utah. I characterized the habitat conditions of known bobolink nesting sites in Utah and compared these conditions to those for nest sites in Wisconsin where bobolinks are abundant. My habitat assessment included identifying vegetation species, vegetation cover, pH, temperature, and precipitation at each site location. Vegetation cover different between Utah and Wisconsin nest sites. Precipitation varied for both locations with no correlation between water availability and bobolink presence. One possible driver for the reduction in bobolinks throughout Utah is the drastic increase in temperature. Other possible external factors include livestock grazing, edge distance, …

Performance, Body Temperature And Blood Metabolites Of Feedlot Steers As Influenced By Environmental Conditions And Supplementation Of Zilpaterol Hydrochloride, Bradley M. Boyd

Department of Animal Science: Dissertations, Theses, and Student Research

Trial one was conducted at the United States Meat Animal Research Center (MARC) near clay center, NE during the summer of 2014. The objective of this trial was to measure the effects of supplementing zilpaterol hydrochloride (ZH) for the final 21 days of the finishing period, and shade, on performance, body temperature, respiration rate, and mobility of finishing beef steers. Feeding ZH increased hot carcass weight, dressing percent, longissimus muscle area, and reduce USDA yield grade. Shade did not affect steer performance and did not reduce body temperature. Zilpaterol hydrochloride increased respiration rate when compared to control cattle and had …

Genomic Analysis Of Sow Reproductive Traits: Identification Of Selective Sweeps, Major Genes, And Genotype By Diet Interactions, Melanie D. Trenhaile

Department of Animal Science: Dissertations, Theses, and Student Research

Reproductive traits, such as litter size and reproductive longevity, are economically important. However, selection for these traits is difficult due to low heritability, polygenic nature, sex-limited expression, and expression late in life. Marker-assisted selection may provide an alternative to increase genetic progress.

Nebraska Index Line (NIL) has been selected for litter size related traits since 1981. It is one of the main contributing lines to the UNL reproductive longevity resource population (n > 1,500), which was genotyped for 60,000 SNPs, phenotyped for age at puberty (AP), lifetime number of parities (LTNP), litter size traits, and other reproductive traits, and fed …

Investigations Into The Cross-Infectivity Of Nematode Parasites Of Cattle And Sheep, Eva Marie Wray

Graduate Theses and Dissertations

The existence of anthelmintic resistance is not singular in parasite species, host species or geographic region. It has become a problem of worldly stature, especially when considering sheep nematodes. Recommendations have been modified from chemical-based control to multipart, managerial intervention. Compared with nematodes of cattle, the worldwide resistance of sheep nematodes to anthelmintic remedies is much more pronounced and urgent. As the resistance to essentially all anthelmintic classes approaches a cautionary level, the implementation of non-chemical controls has grown increasingly essential. Six-6 to 8 month old Holstein steer calves, six-5 month old Katahdin ram lambs and eight-5 month old Suffolk …

Calorimetry And Body Composition Research In Broilers And Broiler Breeders, Justina Victoria Caldas Cueva

Graduate Theses and Dissertations

Indirect calorimetry to study heat production (HP) and dual energy X-ray absorptiometry (DEXA) for body composition (BC) are powerful techniques to study the dynamics of energy and protein utilization in poultry. The first two chapters present the BC (dry matter, lean, protein, and fat, bone mineral, calcium and phosphorus) of modern broilers from 1 – 60 d of age analyzed by chemical analysis and DEXA. DEXA has been validated for precision, standardized for position, and equations and validations developed for chickens under two different feeding levels. These equations are unique to the machine and software in use. Research in broilers …

Autophagy And Its Potential Role In Stress And Feed Efficiency Using Avian Lines, Alissa Laura Piekarski

Graduate Theses and Dissertations

Autophagy is a highly conserved cellular mechanism that is responsible for the degradation and recycling of damaged organelles. Recently, autophagy has been involved in critical roles during overall development of the organism and degradation of damaged cellular components. This pathway has witnessed dramatic growth in the last few years and has been extensively studied in yeast and mammals, however, there is a paucity of information in avian (non-mammalian) species. First, we characterized genes involved in the autophagy pathway in male and female Jungle Fowl to determine gender and tissue specific differences. Secondly, tissue and genotype differences in Japanese quail selected …

Evaluation Of Bacillus Spp Candidates For Aflatoxin B1 Biodegradation In Broiler Chickens, Rosario Galarza Seeber

Graduate Theses and Dissertations

Poultry health has been traditionally maintained by hygenic measures, vaccinations, and the use of antibiotics. Modern husbandry management considers the use of probiotics as a natural way to protect birds against many everyday pathogens. Different strains of Bacillus spp. have proved to have beneficial effects in poultry production. However, the most used bacterium in commercial probiotics is Lactobacillus, a vegetative cell. In contrast, Bacillus spp are bacterial spores, highly resistant to harsh conditions, which makes them preferable, in some cases, to Lactobacillus because of shelf life and storage conditions. There is published information regarding mycotoxin detoxification by bacteria. Mycotoxins are …

Evaluation Of The Impact Of Meat And Bone Meal Nutritional Variability On Broiler Performance, Christopher Caleb Eagleson

Graduate Theses and Dissertations

The objective of this trial was to determine if varying the nutritional composition of meat and bone meal (MBM) would impact broiler performance and yield compared to diets which provide a consistent quality of MBM. Twelve hundred day-old male broilers from the Cobb 500 female line were randomly allocated to 48 pens (25 chicks/pen; 22 square feet) and grown to 49 days of age. Starter, grower, and withdrawal diets were formulated to contain MBM at 10, 7.5 and 5% respectively using the Cobb nutritional standards. Four treatments provided were: 1) H.J. Baker proprietary blend, ProPlus 57 fed continuously; 2) MBM …

The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark

Graduate Theses and Dissertations

Dairy slurry and poultry litter have increased in popularity as fertilizers in the agriculture industry. However, residual effects of these manures on voluntary intake of forages from subsequent harvests are not well known. The objectives of this two part study were to determine if moisture level of alfalfa silage and the use of dairy slurry as a fertilizer have an effect on intake and digestibility by sheep, and if forage species and the use of poultry litter as a fertilizer have an effect on intake by horses. Eighteen ewes (47.6 ± 5.34 kg) were used in experiment 1 and were …

The Physiological Effects And Projected Outcomes Of Urbanization And Pollution On Reptiles, Dale Rodney Lockman

University Honors Program

Research regarding urbanization and its relationship with population fluctuations and physiological responses in animals has been assessed through a multitude of studies involving stress hormone changes, white blood cell counts and other physical and behavioral changes. The scope of this paper emphasizes the explanation of urbanization, the physiological and anatomical impact it has made on reptiles, and the projected outcomes urbanization will have in the future. Some questions are brought up in this paper to serve as a basic overview of what will be learned about urbanization and pollution. In order to provide background information, my research has been conducted …

Dietary Lecithin Supplementation Can Improve The Quality Of The M. Longissimus Thoracis, Darryl N. D'Souza, Bronwyn L. Blake, Ian H. Williams, Bruce P. Mullan, Dave Pethick, Frank R. Dunshea

Animal production and livestock research articles

Forty crossbred (Large White × Landrace × Duroc) female pigs (16.4 kg ± 0.94 kg) were used to investigate the effect of dietary lecithin supplementation on growth performance and pork quality. Pigs were randomly allocated to a commercial diet containing either 0, 3, 15 or 75 g lecithin/kg of feed during the grower and finisher growth phase. Pork from pigs consuming the diets containing 15 g and 75 g lecithin/kg had lower hardness (P < 0.001) and chewiness (P < 0.01) values compared to the controls. Dietary lecithin supplementation at 75 g/kg significantly increased (P < 0.05) the linoleic acid and reduced (P < 0.05) the myristic acid levels of pork compared to the control and the 3 g/kg and 15 g/kg lecithin supplemented treatments. Pigs fed the 75 g/kg lecithin supplemented diet had lower plasma cholesterol (P < 0.05) at slaughter compared to pigs fed the control diet and the 3 g/kg and 15 g/kg lecithin supplemented treatments. These data indicate that dietary lecithin supplementation has the potential to improve the quality attributes of pork from female pigs.

Effects Of Shade And Feeding Zilpaterol Hydrochloride To Finishing Steers On Performance, Carcass Quality, Heat Stress, Mobility, And Body Temperature, Bradley M. Boyd, Steven D. Shackelford, Kristin E. Hales, Tami M. Brown-Brandl, Meredith L. Bremer, Matthew L. Spangler, T. L. Wheeler, D. A. King, Galen Erickson

Department of Animal Science: Faculty Publications

Steers (n = 480; 22% with black hides and 78% with red hides) were used to study the effects of shade and feeding zilpaterol hydrochloride (ZH) on performance, carcass quality, heat stress, mobility, and body temperature (BT). A randomized block design with a 2 × 2 factorial treatment arrangement was used with 4 replicates per treatment. Factors included housing type (open or shaded pens) and the feeding of ZH (0 or 8.33 mg/kg DM) the last 21 d on feed with a 3-d withdrawal. Cattle were blocked by BW into a heavy or light block and randomly assigned to pen …