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Evaluation Of Drug-Resistant Enterobacteriaceae In Retail Poultry And Beef, Agnes Kilonzo-Nthenge, Emily Rotich, Samuel N. Nahashon

Agricultural and Environmental Sciences Faculty Research

There has been increasing concern on the emergence of multidrug-resistant foodborne pathogens from foods of animal origin, including poultry. The current study aimed to evaluate antibiotic-resistant Enterobacteriaceae from raw retail chicken/turkey parts (thigh, wings, breast, and ground) and beef meat (ground and chunks) in Middle Tennessee. Resistance of the collected Enterobacteriaceae to a panel of antibiotics was determined by the Kirby-Bauer disk diffusion test. Retail meats were also assayed for the presence of Salmonella spp. and Escherichia coli O157:H7. Two hundred thirty-seven samples representing 95.2% of the total of 249 samples tested were positive for Enterobacteriaceae. The level of contamination …

American Society Of Parasitologists Newsletter, V. 35, No. 1, Spring 2013, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

American Society of Parasitologists Newsletter, volume 35, number 1, Spring 2013

Status Of Nutria (Myocastor Coypus) Populations In The Pacific Northwest And Development Of Associated Control And Management Strategies, With An Emphasis On Metropolitan Habitats, Trevor Robert Sheffels

Dissertations and Theses

The nutria (Myocastor coypus) is a semi-aquatic rodent native to South America that was introduced to the Pacific Northwest, USA, in the 1930s. Primary damage categories from this invasive species include burrowing and herbivory, resulting in habitat degradation. Nutria have become well-established in metropolitan habitats, and anecdotal information suggests the problem has increased in recent years. However, little regional research on the species has been conducted. The scope of this research, which emphasizes metropolitan habitats, includes three primary foci in relation to nutria populations in the Pacific Northwest: modeling habitat suitability, assessing activity and movement patterns, and identifying and managing …

Development Of A Training Program For The Cal Poly Creamery, Rachel Souerbry

Dairy Science

No abstract provided.

A Business Plan For A Custom Dry Cow Transition Facility And A Calf Ranch, Landon Joseph Fernandes

Dairy Science

In recent economic times the dairy industry has witnessed historic lows in milk price and historic highs in feed prices. As a result many dairies have been forced out of business or have found other ways to diversify in order to offset the accumulated debt from dairying. One possible way a dairy can minimize debt is by sending its transition cows to custom transition cow management facility from dry-off through freshening. This management facility would give optimal care and attention to these cows to ensure metabolic and post calving health issues are minimal and that cows are returned to the …

13. Mathematics, University Of Central Oklahoma

Oklahoma Research Day Abstracts

No abstract provided.

Title Page, Contents, Contributors For Estudios Científicos En El Estado De Hidalgo Y Zonas Aledañas, Volumen Ii, Griselda Pulido-Flores, Scott Monks

Estudios científicos en el estado de Hidalgo y zonas aledañas

Title Page

Contents

Contributors

Los Parásitos Y El Estudio De Su Biodiversidad: Un Enfoque Sobre Los Estimadores De La Riqueza De Especies, Christian E. Bautista-Hernández, Scott Monks, Griselda Pulido-Flores

Estudios científicos en el estado de Hidalgo y zonas aledañas

Durante años los parásitos fueron considerados únicamente como agentes patógenos que debían ser eliminados, sin considerar que son un componente importante de la biodiversidad, que son capaces de aportar información de sí mismos y de otros organismos. Existen estimadores de riqueza que predicen el número de especies para una comunidad a partir de la riqueza observada y de la abundancia de las especies o datos de incidencia; pueden ser estimadores paramétricos y no paramétricos. La comparación de la riqueza de especies entre componente de comunidades de parásitos frecuentemente pueden ser difícil de evaluar debido a que los datos no se …

Animal Health Effects Of The October 2013 Blizzard: Observations, Russ Daly, Ken Olson, Dave Ollila, Dennis Todey, Warren Rusche, Joseph Neary, Dale Miskimins, George Perry

Range Beef Cow Symposium

From Thursday, October 3, through Saturday, October 5, 2013, an unprecedented early season winter storm affected western South Dakota and portions of Wyoming, Montana, North Dakota, and Nebraska. This storm resulted in the deaths of an estimated 15,000 to 30,000 head of cattle, along with many sheep, horses, and other animals. The early occurrence of the storm as well as its severity contributed to direct losses of animals as well as subsequent health effects.

Sustainable Production Of Swine: Putting Lipstick On A Pig?, Michelle B. Nowlin

Faculty Scholarship

No abstract provided.

Physiological Level Production Of Antigen-Specific Human Immunoglobulin In Cloned Transchromosomic Cattle, A. Sano, H. Matsushita, H. Wu, J. Jiao, P. Kasinathan, E. J. Sullivan, Z. Wang, Y. Kuroiwa

Animal, Dairy, and Veterinary Science Faculty Publications

Therapeutic human polyclonal antibodies (hpAbs) derived from pooled plasma from human donors are Food and Drug Administration approved biologics used in the treatment of a variety of human diseases. Powered by the natural diversity of immune response, hpAbs are effective in treating diseases caused by complex or quickly-evolving antigens such as viruses. We previously showed that transchromosomic (Tc) cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin heavy-chain (hIGH) and kappa-chain (hIGK) germline loci (named as κHAC) are capable of producing functional hpAbs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, are homozygously …

Comparison Of Th1 Cytokines And T Cell Markers Gene Expressions Between Virulent And An Attenuated Eiav Vaccine Strain, Talia R. Henkle

Lewis Honors College Capstone Collection

The equine infectious anemia virus (EIAV) is closely related to HIV and has been used as a model to identify protective mechanisms against lentivirus infection. In horses, EIA infection progresses for about a year before infected horses manage to control virus replication. This naturally-gained protection is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. As the resolution of initial viremia correlates with the appearance of virus specific cytotoxic T lymphocytes (CTL), we believe that cellular immune responses play a key role in controlling EIAV in the horse. In …

El Uso De Helmintos Parásitos Como Bioindicadores En La Evaluación De La Calidad Del Agua: Lago De Tecocomulco Vs. Laguna De Metztitlán, Hidalgo, México, Scott Monks, Griselda Pulido-Flores, Christian E. Bautista-Hernández, Berenice Alemán-García, Jorge Falcón-Ordaz, Juan Carlos Gaytán-Oyarzún

Estudios científicos en el estado de Hidalgo y zonas aledañas

Como parte integral de la evaluación de la calidad ambiental del estado de Hidalgo, este estudio tiene énfasis en los helmintos parásitos de los peces y su uso como bioindicadores de la calidad de agua en Lago de Tecocomulco y Laguna de Metztitlán. Como parte del estudio, se obtuvieron los parámetros ecológicos de la infección para cada especie. En Lago Tecocomulco se registraron dos especies de digéneos (larvas), Posthodiplostomum sp. y Diplostomum sp. en el cerebro, los ojos y el mesenterio de dos especies de peces nativos de la zona (Chirostoma jordani y Girardinicthys viviparus); un céstodo (larva), …

Helmintos Parásitos De Algunos Roedores (Mammalia: Rodentia) En San Miguel De Allende, Tepeapulco, Hidalgo, México, Griselda Pulido-Flores, Scott Monks, Jorge Falcón-Ordaz

Estudios científicos en el estado de Hidalgo y zonas aledañas

Los helmintos son un componente de la biodiversidad, que proveen información sobre la historia natural de los hospederos, y de las relaciones tróficas. El conocimiento de la diversidad de roedores y sus parásitos en Hidalgo es escaso y puntual. Por la amplia distribución de los roedores, su adaptabilidad a los ambientes modificados y por el riesgo zoonótico que representan, es importante conocer la parasitofauna de estos hospederos. El objetivo del este trabajo fue registrar la helmintofauna de algunos roedores en San Miguel de Allende, Tepeapulco, Hidalgo, México. Entre agosto de 2005 y junio de 2007 se colectaron 19 roedores (12 …

Análisis Morfométrico De Glypthelmins Quieta (Stafford, 1900) Stafford, 1905 (Digenea: Macroderoididae) En La Reserva De La Biosfera Barranca De Metztitlán, Hidalgo, México, Francisco Alemán-Muñoz, Griselda Pulido-Flores, Scott Monks, Jorge Falcón-Ordaz

Estudios científicos en el estado de Hidalgo y zonas aledañas

Se evaluó la variación morfológica de 30 ejemplares adultos de la especie Glypthelmins quieta, parásitos de Lithobates spectabilis en localidad de Metznoxtla, en la Reserva de la biosfera Barranca de Metztitlán, Hidalgo. El objetivo del presente trabajo fue contribuir al conocimiento de la variación morfológica de G. quieta, y evaluar si los caracteres de presencia ausencia de glándulas perifaringeas y forma de las glándulas mediales son validos para la asignación de ejemplares a una especie. Se generó una base de datos morfológicos realizando tres análisis de componentes principales (ACP). El primer ACP se realizó con la totalidad de …

Análisis De Metales Pesados En Un Céstodo De La Familia Dilepididae, Jorge Falcón-Ordaz, Scott Monks, Griselda Pulido-Flores, Claudia Romo-Gómez

Estudios científicos en el estado de Hidalgo y zonas aledañas

La concentración de plomo (Pb), cadmio (Cd), zinc (Zn), níquel (Ni), hierro (Fe) y aluminio (Al) fue determinada en un céstodo de la familia Dilepididae, parásito de Peromyscus difficilis colectados en una zona rural del Estado de México; con respecto a trabajos previos se obtuvieron valores altos de concentración para Pb y Zn mientras que para Ni sus valores estuvieron por debajo de los valores reportados y el Cd no fue registrado, en lo que respecta al Fe y Al no existen registros de estos metales en céstodos parásitos de organismos terrestres. Con los datos registrados en el presente trabajo …

Nemátodos Parásitos De Roedores De Huehuetla, Hidalgo, México, Jorge Falcón-Ordaz, Scott Monks, Griselda Pulido-Flores

Estudios científicos en el estado de Hidalgo y zonas aledañas

El estudio de nemátodos parásitos de roedores en el estado de Hidalgo es prácticamente desconocido, solamente se han registrado para la entidad un total de 17 especies. Es por esto que se llevó a cabo una recolecta de roedores silvestres en el municipio de Huehuetla, Hidalgo, con el objetivo de conocer los helmintos que parasitan dichos hospederos. Los hospederos se capturaron con trampas Sherman para su posterior sacrificio y revisión helmintológica. Se registra por primera vez Syphacia sp. en Peromyscus mexicanus (Saussure, 1860) como nuevo hospedero. Se ratifica la identificación de Trichuris fossor Hall, 1916 y Hassalstrongylus aduncus Chandler, 1932; …

Estimating The Economic Losses From Diseases And Extended Days Open With A Farm-Level Stochastic Model, Di Liang

Theses and Dissertations--Animal and Food Sciences

This thesis improved a farm-level stochastic model with Monte Carlo simulation to estimate the impact of health performance and market conditions on dairy farm economics. The main objective of this model was to estimate the costs of seven common clinical dairy diseases (mastitis, lameness, metritis, retained placenta, left displaced abomasum, ketosis, and milk fever) in the U.S. An online survey was conducted to estimate veterinary fees, treatment costs, and producer labor data. The total disease costs were higher in multiparous cows than in primiparous cows. Left displaced abomasum had the greatest costs in all parities ($404.74 in primiparous cows and …

Determination Of Newcastle Disease Virus Among Wild Bird Populations In Lake Van Basin, Turkey, Banur Boynukara, Ti̇mur Gülhan, Fethi̇ye Çöven, İlhami̇ Ki̇zi̇roğlu, Ati̇lla Durmuş

Turkish Journal of Veterinary & Animal Sciences

In this study, 540 feces samples taken from a total of 22 bird species, including transit migrant, winter visitor, migratory, and resident bird species, in Lake Van basin were examined by real-time polymerase chain reaction (RT-PCR) with respect to the Newcastle disease virus (NDV). All of the feces samples were cultured in specific pathogen-free embryonated chicken eggs (ECEs) for NDV isolation. The NDV isolates were analyzed for virulent fusion (F) protein by RT reverse transcription PCR (RRT-PCR). Of the 540 examined feces samples, 28 (5.18%) were found to be positive by RT-PCR. The same samples were cultured in ECEs and, …

Effects Of Parity And Litter Size On The Energy Contents And Immunoglobulin G Concentrations Of Awassi Ewe Colostrum, Shogo Higaki, Masashi Nagano, Seiji Katagiri, Yoshiyuki Takahashi

Turkish Journal of Veterinary & Animal Sciences

This study determined the energy content and immunoglobulin G (IgG) concentration of Awassi ewe colostrum and examined the effects of parity and litter size on them. Colostral samples were collected from 57 ewes from the 1st to 7th parities with a litter size of 1 or 2, within 12 h after lambing. Concentrations of colostral fat, lactose, and protein were measured as energy sources, and IgG was measured as representative of the immunoglobulin content of the colostrum. Mean values obtained from analyses of the ewe colostrum samples were 7.4 ± 2.1 kJ/g for the energy value and 60.9 ± 21.4 …

Immunokinetics Of Theileria Equi Specific Antibodies: A Comparison In Serial And Single Dilution Elisa Antibody End Titres, Yogesh Kumar, Dharam Vir Malhotra, Anil Kumar Nichani, Ashok Kumar, Shruti Dhar, Sanjay Kumar

Turkish Journal of Veterinary & Animal Sciences

Theileria equi, a haemoprotozoan of equids, is responsible for endemic infection/latency in tropical/subtropical parts of the world. The present study investigated the immunokinetics of T. equi infection in experimental animals and the diagnostic applicability of single dilution ELISA vis-à-vis conventional serial dilution ELISA. Sequential serum samples were collected up to 90 days postinfection (PI) from 4 donkeys experimentally infected with T. equi. Immunokinetics of T. equi antibodies end titre were determined by serial and single dilution ELISA. A significant rise in initial ELISA antibody end titre (1:800 in serial dilution ELISA and from 1:600 to 1:650 in single dilution ELISA) …

Comparative Evaluation Of Liquid-Phase Blocking Elisa And Solid-Phase Competition Elisa Methods For The Detection Of Antibodies To The Structural Proteins Of Foot-And-Mouth Disease Types O And A Viruses, Murat Şevi̇k, Fahri̇ Ferudun Öztürk

Turkish Journal of Veterinary & Animal Sciences

In this study, we compared 2 methods, liquid-phase blocking ELISA (LPBE) and solid-phase competition ELISA (SPCE), for the detection of antibodies to the structural proteins of foot-and-mouth disease virus (FMDV). These methods were compared using sera collected from cattle (n = 30) without a history of foot-and-mouth disease infection or vaccination, cattle (n = 180) vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera (positive, weak positive, and negative) for FMDV serotypes O and A. The results showed that SPCE had a better specificity (96.67% for serotype O and 100% for serotype A) than LPBE (90% for serotype O and …

Helical Computed Tomography Application In Rabbit Liver Anatomy: Comparison With Frozen Cross-Sectional Cuts, Kamelia Stamatova Yovcheva, Rosen Dimitrov, Yuliana Toneva, Penka Yonkova, Dimitar Kostov, Anton Rusenov, Krassimira Uzunova, Violeta Yordanova

Turkish Journal of Veterinary & Animal Sciences

Our focus has been to study and compare the anatomical helical computed tomography (CT) features of the normal rabbit liver with its native cross-sectional anatomy. Helical CT was used for scanning the cranial part of the abdominal cavity. The slice thickness was 5 mm. Frozen transversal anatomic cross-sections with a thickness of 10 mm were obtained from the cranial abdominal part of 4 animals following euthanasia. They were compared with the corresponding helical CT scans. At Th9 (thoracic vertebra), the helical CT images showed in the whole aspect a normal liver. It was a massive, heterogeneous, soft tissue, with normal …

Microbiological And Parasitological Quality Of Honey Produced In İstanbul, Emek Dümen, Hayretti̇n Akkaya, Gülay Merve Öz, Funda Hati̇ce Sezgi̇n

Turkish Journal of Veterinary & Animal Sciences

In this study, 500 honey samples (with combs) were collected directly from the hives in various parts of İstanbul. The presence and the interactions of different microbiological and parasitological parameters (coliforms, Escherichia coli, Staphylococcus aureus, Ascosphaera apis, Aspergillus flavus, Aspergillus fumigatus, Paenibacillus larvae, Melissococcus pluton, and Nosema spp.) were investigated. According to the results, 80 samples (16%) tested positive for coliforms, 18 samples (3.6%) for E. coli, 67 samples (13.4%) for S. aureus, 51 samples (10.2%) for A. apis, 22 samples (4.4%) for A. flavus, 32 samples (6.4%) for A. fumigatus, 16 samples (3.2%) for P. larvae, 29 samples (5.8%) …

Levamisole Enhances Global And Differential Leukocyte Numbers In Peripheral Blood Of Dogs With Ehrlichiosis, Dhagma Renata Denis Souza, Andreia Lima Tome Melo, Livia Saab Muraro, Daniel Moura Aguiar, Deijanira Albuquerque

Turkish Journal of Veterinary & Animal Sciences

Ehrlichiosis is a disease caused by Ehrlichia, a genus of obligatory intracellular bacteria that parasitize leukocytes and provoke several hematological alterations. In this work, we investigated the effect of levamisole associated with doxycycline on peripheral leukocytes in dogs PCR-positive for ehrlichial DNA. Twenty-one Ehrlichia-infected dogs were randomly distributed in 2 groups: a control group (n = 10) and a levamisole-treated group (n = 11). Blood samples were collected from each animal for hematological and DNA analysis both before initiating the experiment and 5 days later. All dogs were treated with doxycycline (10 mg/kg body weight), cimetidine (5 mg/kg of body …

A Clinical Case: Vitamin C-Responsive Dermatosis In A Holstein Native Cross Bull Calf, Amin Anoushepour, Mehdi Sakha, Pejman Mortazavi

Turkish Journal of Veterinary & Animal Sciences

A male pony foal aged 2 days, reportedly not passing urine since birth and showing moderate signs of colic, was presented for treatment. The clinical examination revealed moderate tachycardia, tachypnea, and mild abdominal distension. Uroperitoneum was confirmed by peritoneocentesis and abdominal fluid-to-serum creatinine ratio (>2:1). After stabilization of the patient, tube cystotomy was performed using infusion set tubing to bypass the urine, allowing the tear on the caudodorsal wall of the bladder to heal itself without suturing. The foal resumed normal urination on day 6 and the catheter was removed 3 days later. Complications were not detected during the …

Assessment Of Amiodarone Effects On Intact Cats' Electrocardiograms, Farzad Gouya, Ali Shabestari Asl

Turkish Journal of Veterinary & Animal Sciences

Amiodarone can be used for treatment of ventricular and supraventricular arrhythmias in canine medicine, but its exact role in feline electrocardiography and its side effects have not been proven. The aim of this study was the evaluation of amiodarone and its different dosages' effects on intact feline heart electrocardiograms. Eight healthy stray cats were selected and control electrocardiogram was obtained. Amiodarone tablets were prescribed twice daily at 10 mg/kg for 7 days. The same protocol was repeated with doses of 15 and 25 mg/kg. Hexagonal electrocardiograms were obtained at the end of each stage. Electrocardiograms were evaluated for P amplitude …

Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Scrapie is a fatal neurodegenerative disease affecting small ruminants that causes economic losses in many countries. It is categorized together with other fatal neurodegenerative diseases as a transmissible spongiform encephalopathy. The aim of this study was to investigate clinical cases and the cause of death in a flock of sheep from Northern Cyprus (Turkish Republic of Northern Cyprus) suspected of having scrapie. Brains from 4 sheep showing clinical signs of classical scrapie, out of a flock of 200 animals, were analyzed by histopathological examination and infective prion enzyme-linked immunosorbent assay (ELISA). The 4 affected sheep were 2-3 years of age …

Detection Of Antibodies Against Parascaris Equorum Excretory-Secretory Antigens, Steffanie V. Burk

Theses and Dissertations--Animal and Food Sciences

Parascaris equorum is a nematode parasite that infects young horses, sometimes causing unthriftiness, respiratory signs, or intestinal impaction in severe cases. Infection can be diagnosed by detection of eggs in feces, but this is only possible after the worms are fully mature. The goal of this study was to develop an antibody-based test for prepatent diagnosis of P. equorum infection. To produce western blot (WB) antigen, P. equorum larvae were cultured for collection of excretory-secretory antigens (ESA). Sera from 18 pregnant broodmares, their subsequent foals, and a group of 12 older mares and geldings were analyzed. In order to check …

Evidence For The Maturation Of Cellular Immune Responses In Equine Infectious Anemia Virus-Infected Ponies, Chong Liu

Theses and Dissertations--Veterinary Science

Equine infectious anemia virus (EIAV) has been used as a model to investigate protective mechanisms against lentiviruses. Unlike other lentiviruses, EIAV replication can be eventually controlled in most infected horses leading to an inapparent carrier state free of overt clinical signs which can last for many years. Maintenance of this carrier state is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. However, the immune mechanisms that are responsible for this control of infection are not yet identified. As the resolution of the initial infection is correlated with the …