Digital Commons Network^™

Genomic Degeneration And Reduction In The Fish Pathogen Mycobacterium Shottsi, David T. Gauthier, Janis H. Doss, M. Lagatta, T. Gupta, R.K. Karls, F. D. Quinn

Biological Sciences Faculty Publications

Mycobacterium shottsii is a dysgonic, nonpigmented mycobacterium originally isolated from diseased striped bass (Morone saxatilis) in the Chesapeake Bay, USA. Genomic analysis reveals that M. shottsii is a Mycobacterium ulcerans/Mycobacterium marinum clade (MuMC) member, but unlike the superficially similar M. pseudoshottsii, also isolated from striped bass, it is not an M. ulcerans ecovar, instead belonging to a transitional group of strains basal to proposed “Aronson” and “M” lineages. Although phylogenetically distinct from the human pathogen M. ulcerans, the M. shottsii genome shows parallel but nonhomologous genomic degeneration, including massive accumulation of pseudogenes accompanied by proliferation of …

Editorial: Tick Saliva: Secret To Blood Feeding Success, Daniel E. Sonenshine, Patricia A. Nuttall, Sukanya Narasimhan

Biological Sciences Faculty Publications

No abstract provided.

Toxic Effects Of Silver Ions On Early Developing Zebrafish Embryos Distinguished From Silver Nanoparticles, Martha S. Johnson, Preeyaporn Songkiatisak, Pavan Kumar Cherukuri, Xiao-Hong Nancy Xu

Chemistry & Biochemistry Faculty Publications

Currently, effects of nanomaterials and their ions, such as silver nanoparticles (Ag NPs) and silver ions (Ag⁺), on living organisms are not yet fully understood. One of the vital questions is whether nanomaterials have distinctive effects on living organisms from any other conventional chemicals (e.g., their ions), owing to their unique physicochemical properties. Due to various experimental protocols, studies of this crucial question have been inconclusive, which hinders rational design of effective regulatory guidelines for safely handling NPs. In this study, we chronically exposed early developing zebrafish embryos (cleavage-stage, 2 hours post-fertilization, hpf) to a dilution series of …

The Acute Physiological Response Of Polar Bears To Helicopter Capture, John P. Whiteman, Henry J. Harlow, George M. Durner, Eric V. Regehr, Steven C. Amstrup, Anthony M. Pagano, Merav Ben-David

Biological Sciences Faculty Publications

Many wildlife species are live captured, sampled, and released; for polar bears (Ursus maritimus) capture often requires chemical immobilization via helicopter darting. Polar bears reduce their activity for approximately 4 days after capture, likely reflecting stress recovery. To better understand this stress, we quantified polar bear activity (via collar‐mounted accelerometers) and body temperature (via loggers in the body core [T_abd] and periphery [T_per]) during 2–6 months of natural behavior, and during helicopter recapture and immobilization. Recapture induced bouts of peak activity higher than those that occurred during natural behavior for 2 of 5 bears, …

All-Inclusive Coral Reef Restoration: How The Tourism Sector Can Boost Restoration Efforts In The Caribbean, Macarena Blanco-Pimentel, Nicolas R. Evensen, Camilo Cortés-Useche, Johanna Calle-Triviño, Daniel J. Barshis, Victor Galván, Erika Harms, Megan K. Morikawa

Biological Sciences Faculty Publications

Following a strong decline in the health of Caribbean coral reefs in the 1970s, disease outbreaks, overfishing, and warming events have continued to push these reefs towards a point of no return. As such, researchers and stakeholders have turned their attention to restoration practices to overcome coral recovery bottlenecks on Caribbean reefs. However, successful restoration faces many challenges, including economical and logistical feasibility, long-term stability, and biological and ecological factors yet to fully understand. The tourism sector has the potential to enhance and scale restoration efforts in the Caribbean, beyond simple financial contributions. Its strengths include long-term presence in several …

Plant-Frugivore Interactions Across The Caribbean Islands: Modularity, Invader Complexes And The Importance Of Generalist Species, Maximilian G.R. Vollstädt, Mauro Galetti, Christopher N. Kaiser-Bunbury, Benno I. Simmons, Fernando Gonçalves, Alcides L. Morales-Pérez, Luis Navarro, Fabio L. Tarazona-Tubens, Spencer Schubert, Tomas Carlo, Jackeline Salazar, Michel Faife-Cabrera, Allan Strong, Hannah Madden, Adam Mitchell, Bo Dalsgaard

Biological Sciences Faculty Publications

Aim: Mutualistic interactions between plants and animals are fundamental for the maintenance of natural communities and the ecosystem services they provide. However, particularly in human‐dominated island ecosystems, introduced species may alter mutualistic interactions. Based on an extensive dataset of plant–frugivore interactions, we mapped and analysed a meta‐network across the Caribbean archipelago. Specifically, we searched for subcommunity structure (modularity) and identified the types of species facilitating the integration of introduced species in the Caribbean meta‐network.

Location: Caribbean archipelago (Lucayan archipelago, Greater Antilles, Lesser Antilles).

Methods: We reviewed published scientific literature, unpublished theses and other nonpeer‐reviewed sources to compile an extensive dataset …

Investigating Public Support For Biosecurity Measures To Mitigate Pathogen Transmission Through The Herpetological Trade, Elizabeth F. Pienaar, Diane J. Episcopio-Sturgeon, Zachary T. Steele

Biological Sciences Faculty Publications

The expanding global trade in herpetofauna has contributed to new infectious disease dynamics and pathways that allow for the rapid spread of pathogens geographically. Improved biosecurity is needed to mitigate adverse biodiversity, economic and human health impacts associated with pathogen transmission through the herpetological trade. However, general lack of knowledge of the pathogen transmission risks associated with the global trade in herpetofauna and public opposition to biosecurity measures are critical obstacles to successfully preventing pathogen transmission. In 2019 we administered a survey to 2,007 members of the public in the United States of America to ascertain their support for interventions …

Trace Mineral Source And Inclusion Level On Wean-To-Finish Pig Production, Marissa Larosae

Electronic Theses and Dissertations

Trace mineral requirements to optimize production for swine are not well defined, leading to excess trace mineral supplementation in swine diets and ultimately to excess trace minerals excreted in swine manure. Potential strategies to reduce the waste of excess trace minerals in diets include utilizing organic sources with higher bioavailability or reducing the overall trace mineral supplementation in the diet. Two studies were conducted in an effort to determine if utilizing organic trace minerals or reducing trace mineral inclusion into diets has an impact on overall performance measurements for nursery and grow-finish pigs, respectively. The first experiment utilized 1,144 weaned …

Snake Reciprocation In Terms Of Classical Conditioning: Scent And Auditory Stimuli, Zeina Y. Ghanem

Undergraduate Research Awards

This study explores the classical conditioning of a snake in order to understand and interpret the physiological responses and behavior exhibited regarding experimental design. The conditioning process involves scent and auditory stimuli where the snake is exposed to running water sounds and a mouse scent. The purpose of this research study is to examine how the snake will later associate the scent of the mouse to the sound and how it would react when sound is the only stimuli administered. The type of snake species used was a young Brook’s Kingsnake. The snake was placed in a test tank and …

Winter Wings: An Introduction To Environmental Education, Samuel G. Mothner

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

Characterizing The Internal Porous Structure Of Equine Proximal Sesamoid Bones Subjected To Race Training Using Fast Fourier Transforms, Joseph Erik Davis

Theses and Dissertations--Biomedical Engineering

The equine racing industry is one of the main proponents in Kentucky’s economic infrastructure. In this industry there has come a need to investigate the nature of the proximal sesamoid bone (PSB). Breakdowns involving the PSBs are the leading cause in racehorse deaths in the industry, with still little known about what causes this bone to fracture. This study seeks to shed insight by investigating the internal structure of the PSB. Using microCT scanning, the internal porous structure was captured. From there, noticeable differences in the pores were noticed and quantified using fast Fourier transform (FFT) analysis.

The dominant peak …

Estrogen Regulation Of Daily Metabolic Rhythms In Female Mice, Oluwabukola Omotola

Theses and Dissertations--Biology

Circadian rhythms are approximately 24-hour cycles of behavior, physiology, and gene expression. In mammals, these circadian rhythms are generated by clocks located in nearly every tissue in the body. The function of circadian clocks is to synchronize physiology and behavior with environmental cycles such as the light-dark cycle. After menopause, when circulating levels of estrogens are very low, women are more susceptible to obesity comorbidities such as metabolic syndrome and cardiovascular disease, suggesting that estrogens regulate these processes. Estrogens could protect females from metabolic dysfunction by regulating circadian rhythms. Consumption of diets that are high in fat contribute to obesity. …

The Microbiome Of The Equine Roundworm, Parascaris Spp., Jennifer Lynn Cain

Theses and Dissertations--Veterinary Science

Parasitic nematodes, including the large roundworms colloquially known as ascarids, affect the health and well-being of livestock animals worldwide. The equine ascarid, Parascaris spp., was the first ascarid parasite to develop wide-spread anthelmintic drug resistance, with other species slowly following suit. There are no new classes of anthelmintics currently in development, and a solution to the ever-increasing prevalence of resistance is desperately needed. The microbiome has been shown to be an important factor in the fitness and health of many organisms and changes to microbiome composition have been associated with a plethora of diseases. The microbiome is also important to …

Spatial Ecology Of Rocky Mountain Elk (Cervus Canadensis Nelsoni) Cows In Southeastern Kentucky, Nathan Dennis Hooven

Theses and Dissertations--Forestry and Natural Resources

The elk (Cervus canadensis) was extirpated from its range in eastern North America by the end of the 1800s, prompting several U.S. states and Canadian provinces to begin translocation programs with the goal of reestablishing elk populations. While eastern elk managers have relied on information from western herds to guide population and habitat management, there is a need for region-specific research on the spatial ecology and habitat associations of translocated elk given the stark differences in landscape, climate, predator communities, and harvest regimes across the continent. While the Kentucky elk reintroduction is one of the best documented programs …

The Response Of Beef Cattle To Disturbances From Unmanned Aerial Vehicles (Uavs), Gabriel Abdulai

Theses and Dissertations--Biosystems and Agricultural Engineering

Unmanned aerial vehicles (UAVs) are increasingly becoming common in animal agriculture. However, research regarding the impact of UAV disturbance on animal wellbeing is lacking or limited. The goal of this study was to investigate the effect of UAV flights on beef cattle by measuring cattle heart and movement rate when introduced to single or multiple UAV flights. A total of 16 -18 crossbred beef heifers were introduced to different flights patterns at between 5 and 9 m above ground level (AGL) at approximately 1 to 2 m/s horizontal velocity for 4 weeks with flights repeated 3 days per week. Results …

Largemouth Bass In The Upper Mississippi River: An Evaluation Of Management Strategies And Understanding Potential Factors Influencing Dynamic Rate Functions, Kylie Beth Sterling

Graduate Theses/Dissertations

The Upper Mississippi River (UMR) supports ecologically and economically important commercial and recreational fisheries. One recreational fishery in the UMR is the Largemouth Bass fishery. Recreational fisheries can be effectively managed using information on population dynamics, though little is known about Largemouth Bass population dynamics in large river ecosystems. Therefore, the objectives of this study were to 1) evaluate recruitment, growth, and mortality of three Largemouth Bass populations in the UMR, specifically within Pools 4, 8, and 13, and 2) to use those estimates of recruitment, growth and mortality to inform exploitation models to evaluate best management practices for each …

Environmental Effects On Biomass Allocation And Small Plot Evaluations Of Aquatic Pesticides For Control Of Nitellopsis Obtusa (Starry Stonewort) Collected From Lake Koronis In Stearns County, Minnesota, Patrick J. Carver

All Graduate Theses, Dissertations, and Other Capstone Projects

Phenology is the study of critical life stages in plants in relation to shifts in environmental factors during seasonal changes. Starry stonewort is a green macro alga in the family Characeae native to Eurasia. Much of the Midwestern United States has been invaded by this species. Starry stonewort has been seen to have late season growth patterns in its invaded range. This study found that this growth pattern was dependent upon water temperature and light transmittance both of which affect biomass production and senescence. It was hypothesized that peak biomass would occur in the late summer (August), however we observed …

Sheepshead (Archosargus Probatocephalus) Neurocranium, Andrew J. Hartmann, Steven Peterson, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from Sheepshead (Archosargus probatocephalus)

Optimization Of Forage Use In Diets Fed To Backgrounding And Finishing Cattle In The Northern Great Plains, Thomas G. Hamilton

Electronic Theses and Dissertations

The two studies in this thesis were conducted to: 1) investigate the impact of corn silage moisture content and kernel processing at harvest on growth performance, efficiency of dietary net energy utilization, and carcass traits in finishing steers when fed at 20% DM inclusion in diets containing modified distillers grains plus solubles; and 2) determine the influence of equal cumulative roughage inclusion in a single diet or twodiet system during a 210-d backgrounding-finishing period in pre-conditioned beef steers on growth performance responses, efficiency of dietary net energy (NE) utilization, and carcass traits. Experiment 1 was a 112-d finishing experiment conducted …

Defining Amino Acid Requirements Of Pregnant Sows: Challenges And Opportunities, Christian D. Ramirez-Camba

Electronic Theses and Dissertations

The efficient use of protein in animal production is dependent on the protein supply and its constituent amino acids (AA) in relation to the animal's needs. Excess AA are deaminated, and the resulting nitrogen (N) is excreted, whereas suboptimal AA intake reduces animal performance. Both increased nutrient excretion and decreased animal performance reduce the overall efficiency of the production unit. In sows, AA requirements should be adequate for optimizing reproductive performance, as measured, for example, by the number of pigs produced per sow per year while limiting N excretion. There is a desire to feed pregnant sows AA levels that …

Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst

Electronic Theses and Dissertations

Because return to angler is an important outcome of put-and-take fisheries, understanding mortality of stocked Rainbow Trout is fundamental to managing these fisheries. Harvest rates of stocked Rainbow Trout Oncorhynchus mykiss in the Black Hills of South Dakota are believed to be below management objectives. Rainbow Trout not harvested by anglers are assumed to be lost to various sources of mortality, raising concerns about the cost of the Rainbow Trout stocking program relative to the benefit provided to anglers. (Simpson 2008). This study evaluated the factors influencing mortality of Rainbow Trout stocked into Black Hills reservoirs. We assessed the effects …

Identification And Characterization Of Candidate Rumen Bacterial Species From Beef Cattle With The Potential For Metabolizing Lipids, Cheyenne E. Hron

Electronic Theses and Dissertations

Ruminants and the microorganisms within their rumen have an intricate symbiotic relationship. Ruminants are able to convert plant materials that are indigestible to humans into meat, milk, and fiber for human consumption and use. Supplementing lipids in diets fed to beef cattle could be beneficial for the host animal as lipids are a higher density energy source. However, they can be detrimental to rumen microorganisms. While much research has been involved in elucidating the composition of the ruminal microbiome, knowledge of the effects of lipids on the ruminal microbiome has been quite limited. In this context, we took advantage of …

Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura

Electronic Theses and Dissertations

The tallgrass prairie of North America has changed drastically since colonial settlement, with up to 99% of this region converted for agriculture and other land-uses. Concurrent with grassland conversion, grassland birds have experienced the most extreme, consistent, and widespread population declines of any avian guild. Agricultural lands in the U.S. Midwest were able to provide adequate habitat for several bird species until the 1950’s; however, altered and intensified management practices have degraded much of remaining suitable habitat and undermined ecosystem functions. Consequently, many grassland birds have been identified as Species of Greatest Conservation Need (SGCN) in many State Wildlife Action …

Pathogen Prevalence In Domestic Sheep In Western Nebraska: Implications For Bighorn Sheep Conservation And Coexistence On A Multi-Use Landscape, Kaytlin Bohr

Electronic Theses and Dissertations

Bighorn sheep (Ovis canadensis) and domestic sheep (Ovis aries) are members of the same genus and thus share multiple pathogens that can be spread between them. One specific respiratory pathogen of concern is Mycoplasma ovipneumoniae (M. ovipneumoniae), which has been linked to pneumonia epizootics in bighorn sheep that are often characterized by all age die offs upon initial exposure followed by years of low lamb recruitment. Domestic sheep have been identified as one carrier of M. ovipneumoniae with transmission between sheep species occurring when there is close contact on the landscape. To prevent this cross-species transmission, importance has been placed …

To Study The Factors Influencing The Sampling Variability On Microbiological Analysis Of Milk And Whey, Ratul Kalita

Electronic Theses and Dissertations

Sampling and analysis are integral parts of dairy processing in quality control, process monitoring and control, and meeting regulatory standards. Accurate sampling is vital to avoid erroneous analysis results and interpretation. The sampling process depends on the appropriateness of sample collection, transportation, storage, and analysis conditions. The bacterial heterogeneity and cluster formation, structural heterogeneity of food, sample unit and size, frequency, and sampling methods, are also crucial in sampling. We aimed to evaluate the influence of sample volume, sampling frequency, and sampling methods in experimental processing lines of whole milk, skim milk, and whey. To standardize the experimental protocols, a …

A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem

Electronic Theses and Dissertations

Lactose is the primary carbohydrate in most mammals' milk, commonly known as milk sugar. Milk permeate is a by-product of whey protein manufacturing through membrane technologies. It is cost-effective, available, and an excellent source of lactose. Sugar phosphorylation is a technique used to alter sugar's characteristics. It has numerous applications for developing dairy and food products. Lactose-6-phosphate (LP) is an organic compound that switches the hydrogen on lactose by monophosphate that has the potential to function as emulsifying salts (ES). ES, such as disodium phosphate (DSP) and trisodium citrate, have a critical effect on the emulsification characteristics of casein by …

Determination Of Some Of The Lectins' Immunohistochemistry Profile Of The Dog Testisand Epididymis, Feyza Başak, Yeşi̇m Akaydin Bozkurt, Roman Dabrowski

Turkish Journal of Veterinary & Animal Sciences

Lectins are substances composed of proteins or glycoproteins that specifically bond to carbohydrates on the cell surfaces or inside the cell. Lectins are used to identify the carbohydrate types in the cellular membrane or cytoplasm. The aim of the study was to identify the location and distribution of the four types of lectins PNA (Peanut agglutinin- Arachis hypogea), WGA (Wheatgerm Agglutinin- Triticum vulgaris), Con A (Conconavalin A-Canavalia ensiformis), and SBA (Soybean Agglutinin-Glycine max) in dog testes and epididymites. The testes and the body of 10 adult dogs were collected from an animal shelter after castration. Following the standard histological procedures, …

Epidemiological Investigation Of Bovine Tuberculosis Infection Dynamics In Turkey, Şahi̇n Çakir, Murat Yildirim, Kadi̇r Serdar Di̇ker, Fevzi̇ye İpek Keski̇n, Selcen Yüksel, Burak Deveci̇, Erhan Akçay

Turkish Journal of Veterinary & Animal Sciences

A national epidemiological research project was carried out to define the dynamics affecting the epidemiology of bovine tuberculosis (bTB) infection in Turkey and to identify the risk factors. Official veterinarian (OV) and breeder original questionnaires were produced separately as part of this study to collect thorough data regarding the disease from the field. The number of questionnaires that needed to be filled out was decided by 95% confidence interval (CI) and 5% margin of error. The findings of 371 OV and 317 breeder questionnaires completed online across the country were analyzed. In addition, 28 outbreaks determined by random method were …

Genetic Analysis Of Biometric Traits In Murrah Buffaloes Using Bayesian Approach, Satpal Dahiyta, Manoj Kumar, Poonam Ratwan, Sandeep Dhillod

Turkish Journal of Veterinary & Animal Sciences

The body conformation traits, which support higher production and longer productive life are very important to breed future dairy buffaloes. Bayesian multiple-trait analysis was performed in biometric traits of Murrah buffaloes to estimate the (co)variance components using an animal model and Gibbs sampling. The model included the fixed effects of parity, season of calving, period of calving, and age group at the time of recording of traits. Random effects included were additive genetic and residual effects. Posterior means of heritability distribution for body length (BL), height at wither (HW), rump width (RW), hip bone distance (HBD), rump slope (RS), brisket …

Effects Of Dietary Calcium, Phosphorus And Microbial Phytase On Intestinal Morphologyin Laying Hens, Sabi̇re Güler, Ender Deni̇z Asmaz, Nuray Varol Kayapunar, İhsan İşbi̇li̇r, Şeri̇fe Şule Cengi̇z, Derya Yeşi̇lbağ, Ahmet Batuhan Şanli, Eyüp Eren Gültepe

Turkish Journal of Veterinary & Animal Sciences

Different challenges are being applied in the poultry industry in order to protect animal health and to increase immunity and production. The supplementation of microbial phytase is essential in terms of both reducing the inorganic phytase rate and contributing to the absorption of other minerals. In this study, a newly isolated microbial phytase was added at different concentrations to the diet together with calcium (Ca$^{2+}$) and available phosphorus (AP), and the effects of this supplementation on intestinal absorption capacity and Ca$^{2+}$ binding capacity were investigated via morphological measurements and immunohistochemical examination of the duodenum and ileum. For this purpose, 90 …