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The Effect Of Spirulina Platensis Algae Inclusion In Feed Of Commercial Broilers Subjected To Cyclic Heat Stress, Kirsten Shafer

Graduate Theses and Dissertations

The effect of Spirulina platensis inclusion in feed of commercial broilers was evaluated over one experiment. The study aimed to investigate the effects of Spirulina platensis freshwater algae inclusion on live performance and processing characteristics of commercial broilers subjected to daily cyclic heat stress. Day old Ross 708 male broilers were placed into 8 environmentally controlled chambers. At d 21, four chambers remained on a control diet containing no algae (CON) while 4 chambers received a diet that included algae at an inclusion rate of 2.5% (ALG). An 8 hour daily cyclic heat stress (24°C to 36°C) was applied to …

Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta

Graduate Theses and Dissertations

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …

Seroprevalence And Titer Concentration Testing For Leptospirosis In Equine, Ashley Himmelsbaugh

Honors College Theses

The most common best management practice used to combat diseases in horses is vaccination, which can decrease the incidence or severity of illness. However, the duration of immunity will vary for each vaccination and must be considered for revaccination purposes. Titer concentration, which evaluates antibodies in the blood, may be a helpful way of determining how long an animal has immunity to a disease. Leptospirosis, a zoonotic, bacterial disease, can result in uveitis, potentially leading to blindness, and abortion in mares. One serovar, Leptospirosis pomona (L. pomona), is associated with most cases of clinical disease in horses in North America. …

Evaluation Of The Selected Nuproxa Feed Additives For Protection Against Lameness And Improving The Wellbeing Of Broilers In The Lameness Challenge Model, Abigail Fanous

Biological Sciences Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a form of bacteria that causes lameness in broiler chickens and is a common concern within the poultry industry. This easily transmissible disease can quickly take over entire livestock of chickens leading to an animal welfare issue. Due to this, it is a pressing issue that must be solved in order to protect the wellbeing of broiler chickens and ensure viable livestock. Nuproxa is a company that specializes in chicken feed in which we used to test different amounts of Panbonis, a dietary supplement, in its effectiveness in protecting broiler chickens against BCO lameness. …

Channel Catfish - Ictalurus Punctatus, Jason E. Rosales, David William Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium total length is 110.81 mm, [CHANNEL CATFISH , Ictalurus punctatus} collected from Canal in Davie on November 18th, 2022 by Zachary

North Coast Otters Public Arts Initiative: Commemorative Auction Catalog, J. M. Black

Archives & Reprint Series (imprint)

In 1999, a citizen science program for submitting river otter observations was created through the Humboldt State University Wildlife department. In 2017, Jeff and Gilly Black from the HSU Wildlife department were inspired by the public arts initiative by The Art of Fundraising project of England's Dartmoor National Park. In 2019, a special partnership with Ink People Center for the Arts, North Coast Open Studios, HSU’s Art Department, Crescent City Art, and the Trinity County Arts Council led to local North Coast artists decorating more than 100 3-foot-tall otter sculptures. The sculptures were displayed at shops, galleries, schools, and other …

Bobcat Identification, Abundance, And Behavior At Road Mitigation Structures In South Texas, Victoria Hanley

Theses and Dissertations

Highways in South Texas fragment the landscape and result in high road mortality rates for the endangered US ocelot (Leopardus pardalis). In response, the Texas Department of Transportation constructed nine wildlife crossing structures (WCS) on Farm-to-Market (FM) 106 which adjoins Laguna Atascosa National Wildlife Refuge in Cameron County, Texas. With ocelots being rare and elusive, bobcats (Lynx rufus) are often used as a surrogate species to study felid behavior at road mitigation structures in South Texas. Bobcats have unique markings which allow them to be individually identified. Three methods of individual identification of bobcats using camera …

Analysis Of Per Capita Contributions From A Spatial Model Provides Strategies For Controlling Spread Of Invasive Carp, Donald R. Schoolmaster Jr., Alison A. Coulter, Jahn L. Kallis, Donald C. Glover, John M. Dettmers, Richard A. Erickson

Natural Resource Management Faculty Publications

Metapopulation models may be applied to inform natural resource management to guide actions targeted at location-specific subpopulations. Model insights frequently help to understand which subpopulations to target and highlight the importance of connections among subpopulations. For example, managers often treat aquatic invasive species populations as discrete populations due to hydrological (e.g., lakes, pools formed by dams) or jurisdictional boundaries (e.g., river segments by country or jurisdictional units such as states or provinces). However, aquatic invasive species often have high rates of dispersion and migration among heterogenous locations, which complicates traditional metapopulation models and may not conform to management boundaries. Controlling …

Wildlife Road Mortality Patterns In South Texas And Survey Methodology Improvement, Bradley E. Beer

Theses and Dissertations

Mortalities of wildlife caused by collisions with vehicles along roads are increasing in prevalence, threatening the existence of various species and populations. A better understanding of how mortalities change in response to natural and anthropogenic variables and efficient methods of obtaining mortality data are essential to mitigating such mortalities. This thesis investigates several key elements to improving road mortality surveys in south Texas. First, it was found that road mortality survey counts did not change under a pandemic-related lockdown and that 2 mortality survey observers detect more mortalities than 1. Analysis of brown pelican groundings on Texas State Highway 48 …

Effects Of Roundup Exposure On Redox Status, Cellular Apoptosis, And Antioxidant And Osmoregulatory Enzyme Expressions In Goldfish (Carassius Auratus), Md Imran Noor

Theses and Dissertations

Intense anthropogenic activities of industrialized nations dramatically increase environmental pollution. This study focused on the effects of Roundup, a glyphosate-based herbicide, exposure (low- and high-dose: 0.5 and 5 mg/L for 2 weeks) on dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), superoxidase dismutase (SOD), catalase (CAT), Na+ /K+ - ATPase (NKA), and renin expressions, and cellular apoptosis in the gills and kidneys of goldfish. Histopathological analysis showed widespread tissue damage in both gills and kidneys. Immunohistochemical analysis provided insights into the expression of molecular biomarkers in tissues. Fish exposed to Roundup exhibited a significant (P<0.05) upregulation in DNP, NTP, SOD, and CAT expressions, and apoptotic nuclei in both tissues. Additionally, exposure to Roundup significantly increased renin expression in kidneys and decreased NKA expression in gills. Overall, our results suggest that exposure to Roundup induces oxidative/nitrative stress and cellular apoptosis and alters osmoregulatory and antioxidant systems which may lead to impaired physiological functions in goldfish.

The Distribution And Reproductive Success Of The Western Snowy Plover Along The Oregon Coast - 2022, David J. Lauten, Kathleen A. Castelein, Daniel Farrar, Mary Lee, Eleanor P. Gaines

Institute for Natural Resources Publications

We monitored the distribution, abundance and productivity of the federally threatened Western Snowy Plover (Charadrius nivosus nivosus) along the central and south coast of Oregon from 1 April – 15 September 2022. We surveyed and monitored plover activity in a project area that included, from north to south, Sutton Beach, Siltcoos River estuary, the Dunes Overlook, North Tahkenitch Creek, Tenmile Creek, Coos Bay North Spit, Bandon Snowy Plover Management Area, New River Habitat Restoration Area (HRA) and adjacent lands, and Floras Lake. Our objectives for the project area in 2022 were to: 1) estimate the size of the adult Snowy …

Understanding Movement, Habitat Use, And Habitat Selection By Largemouth Bass (Micropterus Nigricans X Salmoides) And Alabama Bass (Micropterus Henshalli) On Lake Hartwell, South Carolina, Deon Kerr

All Theses

Alabama Bass (Micropterus henshalli) were illegally introduced into the upper Savannah River Basin 15-20 years ago and have since become abundant as well as problematic ecologically. Acoustic telemetry was used to quantify diel movement, habitat use, and habitat selection of Alabama Bass and Largemouth Bass in Lake Hartwell, SC across seasons (June 2021-July 2022) with the goal of informing ongoing habitat enhancement efforts. Across all seasons, Alabama Bass conducted diel horizontal migrations from deeper offshore habitats during the day to nearshore, shallow habitats at night. Daytime Alabama Bass movement rates in offshore waters were higher than nighttime movement rates. Largemouth …

Divergent Serpentoviruses In Free-Ranging Invasive Pythons And Native Colubrids In Southern Florida, United States, Steven B. Tillis, Jillian M. Josimovich, Melissa A. Miller, Laura L. Hoon-Hanks, Arik M. Hartmann, Natalie M. Claunch, Marley E. Iredale, Tracey D. Logan, Amy A. Yackel Adams, Ian A. Bartoszek, John S. Humphrey, Bryan M. Kluever, Mark D. Stenglein, Robert N. Reed, Christina M. Romagosa, James F.X. Wellehan, Robert J. Ossiboff

United States Department of Agriculture Wildlife Services: Staff Publications

Burmese python (Python bivittatus) is an invasive snake that has significantly affected ecosystems in southern Florida, United States. Aside from direct predation and competition, invasive species can also introduce nonnative pathogens that can adversely affect native species. The subfamily Serpentovirinae (order Nidovirales) is composed of positive-sense RNA viruses primarily found in reptiles. Some serpentoviruses, such as shingleback nidovirus, are associated with mortalities in wild populations, while others, including ball python nidovirus and green tree python nidovirus can be a major cause of disease and mortality in captive animals. To determine if serpentoviruses were present in invasive Burmese …

Characterizing The Cutaneous Microbiome Of Eurycea Lucifuga As A Potential Defense Against Chytridiomycosis, Madeline Key

Senior Honors Theses

Chytridiomycosis is an emerging infectious disease that is significantly reducing global amphibian populations. The disease is caused by Batrachochytrium dendrobatidis (Bd), a fungus that lethally modifies amphibian skin. Recent research has suggested that the cutaneous microbiome of individual amphibians may play a role in susceptibility to the pathogen. In this study, twelve cave salamanders (Eurycea lucifuga) were collected. Cutaneous bacteria from each salamander were isolated and identified using Sanger Sequencing. Additionally, a Bd-challenge assay was performed to determine each isolate’s antifungal activity. Results indicated many microbial isolates possessed inhibitory capabilities against Bd, which may …

South Texas Wildlife Activity Across A Fragmented Landscape And Road Mitigation Corridor, Caitlin K. Brett

Theses and Dissertations

Wildlife crossing structures (WCS) and roadside fencing are commonly installed to mitigate habitat fragmentation, wildlife road mortalities, and other negative effects that roads can have on the surrounding landscape. Eight such WCS were constructed below Farm-to-Market (FM)106 in Cameron County, Texas, across a 16 km corridor transecting the Laguna Atascosa National Wildlife Refuge. These WCS, paired with adjacent roadside fencing, were intended to prevent road mortalities of the endangered ocelot (Leopardus pardalis) and to mitigate the barrier effect of FM106 on this and other meso-mammal species. This study will analyze camera trap data from roadside and habitat reference …

Beef Bull Selection And Management Affecting Breeding Season Outcomes For California Cattle Producers On Rangelands, Megan Banwarth

Master's Theses

California’s approximately 660,000 head of beef cattle are highly dependent on range bulls used to produce offspring that can perform across the state’s diverse ecological regions. While knowledge of bull breeding behavior and the technology used to monitor cattle movement and behavior is critical to maximize conception rates and the overall efficiency in natural service sires on rangelands. Bulls need to be functional on rugged coastal landscapes, rolling foothills, deserts, and in high-elevation terrain. Few data exist that indicate factors related to selection, maintenance, and longevity of bulls used in rangeland landscapes. Objectives herein, were to assess factors influencing bull …

The Impacts Of A Quagga And Zebra Mussel Infestation, Casey Silva

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Invasive species issues have been on the rise in the United States for decades. These organisms can disrupt the natural flow of an ecosystem and overtake native species, altering an environment as a whole. The introduction of the zebra mussel (Dreissena polymorpha) in 1988, followed by the quagga mussel (Dreissena rostriformis) in 1989 is arguably the most prolific aquatic infestation the nation is currently up against (Hoddle, 2022). Beginning in the Great Lakes, both quagga and zebra mussels quickly spread their infestations through the Midwest and the East coast. The potential invasion of these species across …

Evaluation Of Extended Aging Type And Cooking Practices On Palatability Of Beef Steaks, Katharine Anne Bugenhagen

Graduate Theses and Dissertations

Two studies were conducted to determine if sous vide was a viable way to improve palatability of various beef cuts, and to determine whether extended aging duration and the presence of a bone impacts the consumer ratings of beef short loins.When evaluating the impact of sous vide cooking (SVG) on multiple beef muscles, there was a muscle × method interaction for overall liking (P = 0.04). The traditionally cooked (TRAD) Longisimus lumborum (LL) was rated the highest (P < 0.05). The Chuckeye steak (CHE) was rated next, both TRAD and SVG, with the traditional ranked higher, this was followed by the Triceps brachii (TB) also for both TRAD and SVG, however for the TB the SVG ranked higher (P < 0.05). The TRAD Biceps femoris (BF) rated the lowest (P < 0.05) of all of the muscles. Shear force did no differ between the cooking treatments (P > 0.05), or was there an interaction between muscle × method for shear force measurements (P < 0.05). The LL was rated the highest for tenderness (P < 0.05) while the BF was rated the lowest (P < 0.05). The second study compared subprimals aged over three aging periods (21, 42 63 days), then split into bone-in or boneless steaks, there were no interactions observed within the consumer data (P ≥ 0.05). BI steaks were rated higher (P < 0.05) for juiciness and overall liking by consumers, however BI and BL were rated similarly by consumers for tenderness and flavor (P > 0.05). When evaluated by consumers, aging did …

Amino Acid Nutrition In Broiler Breeders, Jordan Weil

Graduate Theses and Dissertations

Amino acids (AA) are commonly referred to as the building blocks of protein due to their role in the synthesis of proteins. Considering these nutrients are involved in growth and metabolism, much emphasis is placed on determining the dietary requirements of amino acids. Therefore, an experiment was conducted to evaluate the amino acid requirements of performance parameters such as egg weights, egg production, egg mass, feed conversion ratios, and body weight gain in two age groups of broiler breeders. In this experiment, 648 Cobb 700 broiler breeder hens were placed in individual cages to determine the amino acid requirements of …

Optimization Of Clostridium Septicum Antigen Production And Evaluation Of Vaccine Administration Parameters For A Candidate Bacterin-Toxoid To Prevent Dermatitis In Commercial Turkeys, Aaron Forga

Graduate Theses and Dissertations

The objective of this thesis was to optimize the hemolytic activity of two isolates of Clostridium septicum to evaluate if hemolytic titer of C. septicum antigen at time of formalin inactivation corresponded to increased serum antibody titer to the C. septicum alpha-toxin of turkeys immunized with an experimental formalin inactivated bacterin-toxoid vaccine. Chapter 1 provides a brief overview of the thesis. Chapter 2 consists of a literature review focused on clostridial dermatitis, specifically C. septicum-associated dermatitis (cellulitis) that affects commercial turkeys. Chapter 3 outlines our attempts to optimize an experimental autogenous bacterin-toxoid vaccine as a potential strategy to mitigate clostridial …

Molecular Mechanisms Underlying Aberrant Circulating Steroid Hormones In Gnrhr-Ii Knockdown Boars And Their Control Littermates, Dorothy H. Elsken

Department of Animal Science: Dissertations, Theses, and Student Research

Swine are the only livestock species that produce a functional protein for both GnRH-II and its receptor (GnRHR-II). Our laboratory reported that the interaction between GnRH-II and GnRHR-II facilitates testosterone production, independent of LH stimulation. To further examine GnRHR-II function, we developed a swine line with 70% reduced GnRHR-II mRNA levels (GnRHR-II KD) compared to control littermates. Mass spectrometry analysis of serum revealed that circulating levels of gonadal steroids were reduced in mature transgenic vs. control boars. Paradoxically, evaluation of testicular tissue indicated no difference between lines for gonadal steroid hormone levels. To address a potential cause for this discrepancy, …

The Invisible Meat Microcosmos: Investigations Of Processed Meats' Specific Spoilage Organisms, Rebecca Furbeck

Department of Animal Science: Dissertations, Theses, and Student Research

As humankind grows in population, so will our need to compete against bacterial populations which spoil foodstuffs before we are able to consume them. This dissertation aims to identify the bacterial communities responsible for meat spoilage, and the mechanisms that govern the behaviors of these organisms. The literature review summarizes the state of knowledge regarding factors that influence meat microbiome composition, and how analytical methods can influence research outcomes. Study 1 aimed to identify the impact of additional ingredients and processing steps on the microbial ecology of processed turkey products. The main specific spoilage organisms (SSOs) belonged to the orders …

Impacts Of Feeding Biochar To Beef Cattle On Greenhouse Gas Emissions And Performance And Characterizing Yearling Steers Grazing Smooth Bromegrass Pasture Using Gps, Holly A. Heil

Department of Animal Science: Dissertations, Theses, and Student Research

As global warming continues to rise, the pressure and growing demand for sustainable animal production is increasing. Livestock naturally produce greenhouse gas emissions through enteric fermentation. Dietary manipulation strategies have been sought out to decrease emissions in ruminants using feed additives.

Two independent finishing feedlot experiments evaluated two types of biochar and its effects on animal performance, carcass characteristics and greenhouse gas emissions. Experiment 1 utilized pistachio shell biochar and experiment 2 utilized biochar sourced from ponderosa pine wood waste, both types included at 1% of the diet DM. The addition of biochar to the diet did not impact animal …

The Nebraska 4-H Equine Advancement Level Program’S Role In Positive Youth Development Using The Five Cs Model: An Exploratory Study, Eunhye Mccarthy

Department of Animal Science: Dissertations, Theses, and Student Research

Positive youth development (PYD) focuses on developing the strengths of youth through positive activities and behaviors rather than reducing negative tendencies. A well-researched model of PYD is the five C’s model that is popularly used in youth development organizations such as 4-H. The Nebraska 4-H Equine Advancement Level program was intended to serve as a guidebook for instruction and assessment of youth as they learn more about horses and horsemanship skills. Each of the four levels has an emphasis in developing a 4-H member’s responsibility, self-discipline, self-reliance, patience, and pride of accomplishments. The program was started approximately 30 years ago …

Variance Component Estimates For Growth Traits In Beef Cattle Using Selected Variants From Imputed Low-Pass Sequence Data, Chad A. Russell

Department of Animal Science: Dissertations, Theses, and Student Research

A beef cattle population (n = 2,343) was used to assess the impact of variants identified from imputed low-pass sequence (LPS) on the estimation of variance components and genetic parameters of birth weight (BWT) and post weaning gain (PWG). Variants were selected based on functional impact and were partitioned into four groups (Low, Modifier, Moderate, High) based on predicted functional consequences and re-partitioned based on consequence of mutation, such as missense and untranslated region variants, into six groups (G1-G6). Each subset was used to construct a genomic relationship matrix (GRM) for univariate animal models. Multiple analyses were conducted to …

Environmental Determination Of Gastrointestinal Parasitism In Katahdin Sheep, Brian Arisman

Department of Animal Science: Dissertations, Theses, and Student Research

Gastrointestinal nematodes (GIN) negatively affect the performance and wellbeing of sheep and are difficult to control due to anthelmintic resistance. Producers are therefore choosing breeds naturally resistant to GIN infection, such as the Katahdin. Katahdin are raised in various climates and management systems in the U.S., and those environmental factors can be combined into eco-management clusters. We hypothesized that GIN challenge varies predictably based on the characteristics of these clusters. Katahdin producers from across the U.S. were surveyed for management information. Body weights (BW), fecal egg counts (FEC) and FAMACHA scores (FAM) were collected on 3,527 Katahdin lambs around 90 …

Evaluation Of Nutritional Factors Affecting Sow Reproductive Longevity, J'Nan E. Wittler

Department of Animal Science: Dissertations, Theses, and Student Research

Sow reproductive longevity is an important trait for swine operations from a production and economic standpoint. Previous research has shown that a sow needs to produce 3 litters before she covers the costs associated with introducing her to the operation. A literature review of research conducted related to sow longevity is discussed in Chapter 1. Chapters 2 and 3 present data from the University of Nebraska – Lincoln’s long-term sow longevity research. The research looks at the effects of energy restriction with soy hulls during gilt development on sow reproductive longevity through 4 parities. Chapter 2 presents data from replications …

Students' Perceptions Of Online Equine Courses And Their Impacts On Learning Outcomes, Blaire L. (Gibbens) Speck

Department of Animal Science: Dissertations, Theses, and Student Research

Post-secondary education is always changing and evolving. Over the last few years, significant changes in education have resulted in an increased number of online courses. Approximately 28% of students seeking higher education participate in at least one online course and 14% are enrolled exclusively in distance or online programs (Allen & Seaman, 2016). However, courses that are typically hands-on, like equine science, may be more challenging online. The hands-on experiences in equine science classes help prepare students for future careers. Due to an increase in students choosing to take courses online, a review of online teaching methods was conducted to …

Gut Microbiota Shift In Layer Pullets Fed On Black Soldier Fly Larvae-Based Feeds Towards Enhancing Healthy Gut Microbial Community, Evalyne W. Ndotono, Fathiya M. Khamis, Joel L. Bargul, Chrysantus M. Tanga

All Peer-Reviewed Publications

Globally, most gut microbiota-related studies have focused on broilers due to their diverse microbial communities compared to that of layer chicken. However, in Africa few studies have been undertaken despite the increasing benefits to the poultry industry. The utilization of Insect-Based diets to improve the gastrointestinal function and gut health in poultry is increasingly gaining global attention. Here, we evaluated the potential roles of commercial black soldier fly larvae-based feeds (BSFLF) in reshaping the abundance, composition and diversity of the gut microbiota of layer chickens using high throughput Oxford nanopore Minion sequencing of the full length bacterial 16S rRNA gene. …

Enhancing Amino Acid Utilization For Broiler Chickens Under Different Scenarios Of Dietary Protein Reduction And Subclinical Coccidiosis, Derrell Trevor Lee

Graduate Theses and Dissertations

Five experiments were conducted to evaluate responses of broiler chickens fed diets with different dietary amino acid (AA) levels when fed normal and reduced dietary crude protein (RCP) diets and reared under different coccidiosis control programs. In the studies with the use of RCP diets, the relationship between dietary Gly + Ser and the Met:Cys ratio was investigated. Experiment 1 evaluated broilers in 2 coccidiosis control programs (live vaccine or in-feed zoalene) and fed increased dietary AA derived from soybean meal or unbound sources from 0-40 d. Vaccination impaired young (0-26 d) broiler body weight gain (BWG) and feed conversion …