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Profiling The Cell Wall Polysaccharides Of Hemp (Cannabis Sativa L.) Inflorescence And Hempseed And Exploring Their Application As Ruminant Feed Ingredients, Miranda Agbana

Theses and Dissertations--Animal and Food Sciences

Hemp (Cannabis sativa L.) is used in the United States as a source of CBD oil, fiber, and grain. While hempseeds are consumed whole or as dehulled hemp hearts, hempseed oil is also a desirable seed oil in human nutrition. The process of collecting hempseed oil leaves behind hempseed cakes that are abundant in protein and fiber. Feeding of hempseed cakes to animals, including ruminants, has only initially been explored. Extraction of CBD from hemp inflorescences generates organic waste that may find a second purpose as a fiber feed for cattle. Little is known about the exact fiber composition …

Precision Livestock Farming And Personality As Tools To Understand Dairy Calf Individual Variation In Performance, Behavior, And Response To Stress, Megan Marie Woodrum Setser

Theses and Dissertations--Animal and Food Sciences

Individual differences impact animal responses to their environments and can be observed with animal personality (stable behavioral differences with populations) and food neophobia (avoidance of unfamiliar feeds). These individual differences can influence cattle performance and welfare. Precision technologies, such as automatic feeding systems (AFS) and accelerometers continuously measure feeding behaviors and activity and may allow us to relate individual differences to behaviors in the home environment. Therefore, the first objective was to determine if food neophobia was associated with feeding behaviors measured by AFS. Food neophobia was associated with rewarded visits to the AFS, but to no other feeding behaviors. …

Telomeres: A Tool To Assess The Impacts Of Mining Contaminants On Riparian Songbirds, Lillian Krach, Bridger Creel, Megan Fylling, Zac Cheviron, Creagh Breuner

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Mining has left massive environmental and physical scars across the landscape. Aquatic and riparian landscapes in particular have been significantly impacted by traditional mining practices. Waste products left over from hard-rock mining leech heavy metals onto the landscape and these metals spread from headwater streams to major waterways (Lottermoser 2010). Heavy metals have been shown to cause physiological stress and challenges to organisms depending on the metal and the concentration (Baos et al. 2019, Boyd & Rajakaruna 2013). While some mining-impaired areas have undergone restoration efforts, is it enough? Typical restoration methods replace the contaminated floodplain, but not the riverbed …

Predators In Paradise: Ecotourism And Predator-Prey Dynamics In Monteverde, Costa Rica, Andrew Cremeans

Undergraduate Theses, Professional Papers, and Capstone Artifacts

The use of camera trap surveys is increasingly common to investigate recurring diel activity of mammals. Investigation into the temporal overlap of mammals can provide unique insights into predator-prey dynamics. Understanding this relationship is essential to effectively manage and conserve both species. Extensive research across the tropics has found that daily activities of mammals were shaped by thermoregulation and trophic location in food webs. Although broad scale studies of daily patterns of mammals have enhanced our understanding of these constraints, many study sites included were remote protected areas under strict conservation measures. Such protected areas often had full complements of …

Determining Species-Specific False-Positive Rates Using Visual And Auditory Cues: A Case Study With Sagebrush Steppe Songbirds, Amelia K. Evavold

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Errors in wildlife field data threaten to bias resulting abundance and occupancy estimates if not properly accounted for or minimized. Methods to account for false-positive errors in wildlife data have not been as thoroughly developed as those for false-negative errors despite false-positives being present across diverse wildlife taxa and study systems. The calibration method to account for false-positives involves assessing the field detection method to determine how often false-positive errors occur in the field data. Rates can then be incorporated into estimations based on the field data to improve estimation accuracy. This study presents an application of the calibration approach …

A Single-Cell Atlas Of Bovine Skeletal Muscle Reveals Mechanisms Regulating Intramuscular Adipogenesis And Fibrogenesis, Leshan Wang, Peidong Gao, Chaoyang Li, Qianglin Liu, Zeyang Yao, Yuxia Li, Xujia Zhang, Jiangwen Sun, Constantine Simintiras, Matthew Welborn, Kenneth Mcmillin, Stephanie Oprescu, Shihuan Kuang, Xing Fu

Computer Science Faculty Publications

Background

Intramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of using beef cattle as a novel model for mechanistic studies of intramuscular adipogenesis and fibrogenesis.

Methods

Skeletal muscle single-cell RNAseq was performed on three cattle breeds, including Wagyu (high IMF), Brahman (abundant IMC but scarce IMF), and Wagyu/Brahman cross. Sophisticated bioinformatics analyses, including clustering analysis, gene set enrichment analyses, gene regulatory network construction, RNA velocity, pseudotime analysis, and cell-cell communication analysis, were performed to elucidate …

Ancient Bears Provide Insights Into Pleistocene Ice Age Refugia In Southeast Alaska, Flavio Augusto Da Silva Coelho, Stephanie Gill, Crystal M. Tomlin, Marilena Papavassiliou, Sean D. Farley, Joseph A. Cook, Sarah A. Sonsthagen, George K. Sage, Timothy H. Heaton, Sandra L. Talbot, Charlotte Lindqvist

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

During the Late Pleistocene, major parts of North America were periodically covered by ice sheets. However, there are still questions about whether ice-free refugia were present in the Alexander Archipelago along the Southeast (SE) Alaska coast during the last glacial maximum (LGM). Numerous subfossils have been recovered from caves in SE Alaska, including American black (Ursus americanus) and brown (U. arctos) bears, which today are found in the Alexander Archipelago but are genetically distinct from mainland bear populations. Hence, these bear species offer an ideal system to investigate long-term occupation, potential refugial survival and lineage turnover. Here, we present genetic …

Movement And Genomic Methods Reveal Mechanisms Promoting Connectivity In A Declining Shorebird: The Lesser Yellowlegs, K. Christie, R. E. Wilson, J. A. Johnson, C. Friis, C. M. Harwood, L. A. Mcduffie, E. Nol, Sarah A. Sonsthagen

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

No abstract provided.

Fecal Dna Metabarcoding Shows Credible Short-Term Prey Detections And Explains Variation In The Gut Microbiome Of Two Polar Bear Subpopulations, M. Franz, L. Whyte, T. C. Atwood, D. Menning, Sarah A. Sonsthagen, S. L. Talbot, K. L. Laidre, E. Gonzalez, M. A. Mckinney

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

No abstract provided.

Habitat Use By Sandhill Cranes Wintering In The Agricultural Landscape Of The Sacramento–San Joaquin River Delta Of California, Gary L. Ivey, Bruce D. Dugger, Caroline P. Herziger, Michael L. Casazza, Joseph P. Fleskes

Proceedings of the North American Crane Workshop

The Sacramento–San Joaquin Delta region of California is an important wintering region for sympatric greater (Grus canadensis tabida) and lesser (G. c. canadensis) sandhill cranes. Basic information about sandhill crane use of habitats in their winter landscape is needed to design a biologically driven conservation strategy. We monitored a sample of radio-tagged birds of each subspecies for 2 winters and conducted foraging counts to document their habitat use. Cranes used the major crops and habitat types that were available in the landscapes surrounding their roost sites and focused most of their foraging in grain crops. We examined habitat selection surrounding …

Whooping Crane Nesting Behavior, Parental Care, And Ecology At Wood Buffalo National Park, Andrew J. Caven, Michael J. Forsberg

Proceedings of the North American Crane Workshop

We studied Whooping crane (Grus americana) nesting behavior and movement patterns at Wood Buffalo National Park using an instantaneous scan sampling approach. We also photo-documented important behavioral and ecological observations. Data were collected from 2 to 9 June 2023 from before 3:00 to after 22:00 hours. We completed 88 hours of observation and documented nest swapping about every 3.2 hours. The female incubated/brooded 3.3-10.9% more than the male, and the probability of incubation/brooding declined by 49% after the second chick began pipping and by 99% after both chicks had hatched. The eldest chick was successfully provisioned 16 hours and 33 …

Inland Habitat Selection Model For Wintering Whooping Cranes, Karen Tyrell, Kristin Brightwell, Matthew Crane, Wade Harrell, Rosa Palmer, Aaron T. Pearse, Ted Owen

Proceedings of the North American Crane Workshop

Inland habitat use by wintering Aransas-Wood Buffalo whooping cranes (Grus americana) is expected to increase given projected population growth and observations of some whooping cranes using inland winter habitat in addition to coastal marshes. We developed resource utilization functions using ‘random forests’ to model whooping crane use as a function of environmental covariates considered important for whooping crane use. Covariates associated with distance to cropland, distance to development, and wetness or standing water were the most influential in model prediction. The model estimated that the 50% predicted use contour encompassed 34,925 hectares (ha) and the 95% predicted use contour encompassed …

Mortality Of Fledged Whooping Cranes In The Aransas-Wood Buffalo Population, Kristin Brightwell, Matt Rabbe, Aaron T. Pearse, Ben Bainbridge, Karen Tyrell, Clayton Derby

Proceedings of the North American Crane Workshop

Maintaining accurate mortality records for endangered species is important for guiding management and conservation actions. Mortalities of whooping cranes (Grus americana) in the Aransas-Wood Buffalo Population (AWBP) are documented in published records from 1950 through 2010. This paper documents publicly available records of known mortalities from 2011 through 2023, and updates historic records from 1950 to 2010. Public sightings and telemetry carcass records were restricted to fledged whooping cranes from the AWBP and stratified by confirmed, suspected, and unknown causes across the annual range (i.e., summer, migration, and winter). This mortality update provides more certainty associated with available carcass data …

Differential Habitat Use Of Wintering Whooping Cranes Throughout The Range Of The Eastern Migratory Population, Hillary L. Thompson, Anne E. Lacy, Robert F. Baldwin, Patrick G. R. Jodice

Proceedings of the North American Crane Workshop

In 2001, a reintroduced population of whooping cranes (Grus americana), the Eastern Migratory Population (EMP), was established in the eastern United States. There has been no assessment of habitat use of the EMP across the current winter distribution. During 2 winters, we used radio-telemetry to track groups of cranes each for 1 day. We grouped sites into 3 regions, based on natural clustering: north (Illinois, Indiana, Kentucky), central (Tennessee, Alabama), and south (Georgia, Florida, Louisiana). Home ranges decreased in size from north to south (4.9 ± 2.8, 3.1 ± 1.0, and 2.3 ± 0.5 km2, respectively). In the north and …

Comparisons Of Migration Patterns Between Parent-Reared And Costume-Reared Whooping Cranes In The Eastern Migratory Population, 2012–2023, Mary Anderson, Glenn H. Olsen, Susie Dunham, Hillary L. Thompson, Marianne M. Wellington

Proceedings of the North American Crane Workshop

Whooping cranes (Grus americana) have been raised and released into the Eastern Migratory Population (EMP) since 2001. Cranes either were costume-reared and learned their migration route by following an ultralight aircraft, were costume-reared and released in fall near adult cranes (known as direct autumn release), or were parent-reared by captive adult whooping crane pairs and released in the fall near adult whooping cranes. Compared to costume-rearing, parent-rearing encourages conspecific learning prior to release, but needs further research to understand how it may influence post-release behaviors including migration. This study compared the migratory timing and length of parent-reared whooping cranes and …

Temporal Patterns Of Sandhill Crane Use Of The Pecos River Valley, Chaves County, New Mexico, James B. Montgomery Jr.

Proceedings of the North American Crane Workshop

The Pecos River Valley is an important stopover area for both migrating and wintering sandhill cranes (Grus canadensis) in southeastern New Mexico. The combination of shallow impoundments and a playa lake at Bitter Lake National Wildlife Refuge, off-refuge shallow ponds and riverine wetlands, and approximately 20,000 ha of nearby irrigated farmlands in Chaves County provided roosting and foraging areas used by the cranes. Once per week sandhill cranes were counted when departing their roosts during September through April 2005–2024. The fall migration during October and November peaked at 24,431 ± 204 (SE). During this peak period, cranes divided foraging between …

Movement Of Eastern Migratory Whooping Cranes Outside The Nonessential Experimental Population Range, 2002–2024, Nicole M. Gordon, Eleanor A. Laack, Hannah R. Macinnes, Alicia M. Ward, Hillary L. Thompson

Proceedings of the North American Crane Workshop

Whooping cranes (Grus americana) are listed as an endangered species under the Endangered Species Act (ESA), with 1 historic migratory population and multiple reintroduced populations. The reintroduced populations, including the Eastern Migratory Population (EMP), are considered nonessential experimental populations (NEP) under section 10j of the ESA. Within the boundaries of the NEP range, whooping cranes are treated with the protection of a threatened species on federal lands but are treated as a species under consideration for listing on private lands. However, if an individual exits the NEP range, they are treated as federally endangered and have the full protections of …

Proceedings Of The Sixteenth North American Crane Workshop: Abstracts, Megan E. Brown, Barry K. Hartup, Jane E. Austin

Proceedings of the North American Crane Workshop

Following is a list of all abstracts of presentations given at the 16th North American Crane Workshop (2023), some of which were not published in these Proceedings.

Proceedings Of The Sixteenth North American Crane Workshop, 23–26 October 2023, Baraboo, Wisconsin, Cover And Frontmatter, Megan E. Brown, Barry K. Hartup, Jane E. Austin

Proceedings of the North American Crane Workshop

Cover and frontmatter for the Proceedings of the Sixteenth North American Crane Workshop held October 23–26, 2023 in Baraboo, Wisconsin. Includes the preface by the editors, in memoria for M. Cathy Nowak (1955–2023) and Geroge F. Gee (1937–2022), and the contents.

Entry Assessment Of Salmonella And E. Coli Contamination In Poultry Feed On The Farms, Ilham Ardiansah

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research was aimed at precisely estimating the probabilities associated with the presence of Salmonella and E .coli contamination in feed at the entry of broiler and broiler breeder farms. The samples were taken from four different farms during six weeks located in Chonburi Province, Thailand. The microbiological analysis was conducted in the Laboratory of Microbiology, Regional Medical Science Center, Saraburi, Thailand. The entry assessment followed the concept of import risk analysis, including determining models for probabilistic prevalence and the probability of entry. The statistical model used a beta distribution followed by Monte Carlo simulation to assess probabilistic prevalence and …

Comparing Fish Species Assemblage Between Impacted And Restored Estuarine Salt Marshes Within The Eel River Estuary, Humboldt Co. Ca, Adam Aguilar, Isaac Basham-Clair, Tristan F. Parkinson, Larkin C. Wells

Undergraduate Scholarship

This report details a study assessing the fish assemblages of a recently restored estuarine salt marsh site, and a similar unrestored and impacted site along the Eel River estuary. These two sites are located within the Eel River (Wiya’t) estuary, roughly 10 miles west of the city of Fortuna, CA. This study was conducted during the months of September through October 2023. Data collection was done through seine net dragging at randomly selected sample points across both sites. Seine net hauls were recorded by species and species count, which were later computed using a diversity index to provide values representative …

A Preliminary Assessment Of The Spawning Potential Ratio Of Five Target Species Of The Coastal Gillnet Fishery In Guyana And Suriname, Tomas Willems, Elford Liverpool, Michael Hiwat

Gulf and Caribbean Research

Data—limited stock assessment methods have the potential to positively impact livelihoods of fishermen around the world by providing management recommendations that aim to optimize sustainable yields from fisheries. Some years ago, a novel length— based (LB) method was developed for the assessment of spawning potential ratio (SPR), a measure of the reproductive capacity of fish stocks. We applied the LB—SPR method to 5 important target species of the coastal gillnet fishery in Guyana and Suriname. Nebris microps (Smalleye Croaker; 32% SPR) and Macrodon ancylodon (King Weakfish; 34% SPR) had the highest spawning potential, but remained below the 40% target level …

Dog Weight, Age, And Sex Relationship With Shelter Length-Of-Availability At Pope Memorial Humane Society, Logan Lajoie

Honors Theses and Capstones

Many factors can influence length-of-availability for dogs in shelters, ranging from factors of the dogs themselves to outside factors. The external factors could be the geographical location of the shelter and its clientele, the socioeconomic status of the area, how the individual shelter uses its resources, the region's policies, and more (Scarlett, 2021, p. 18). This study evaluated length-of-availability in dogs at the Pope Memorial Humane Society, a nonprofit animal shelter that serves Strafford County, New Hampshire. This allowed for dog-specific factors to be investigated more closely. The specific factors investigated were dog weight, age, and sex compared to their …

Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery

College of Graduate Studies: Theses & Dissertations

Since the 1980s, non-algal aquaculture has grown to encompass 49% of all seafood production in response to a growing human population and increased seafood demand (FAO, 2022). Hurdles exist to aquaculture sustainability, including dependence on wild sourced fishmeal (FM) and the impacts wastewater discharge. It takes 4-5 tons of wild forage fish to produce one ton of dry FM (Miles and Chapman, 2006) and as aquaculture is primarily conducted in earthen ponds and public open water bodies (FAO, 2022), finfish culture can have a high impact on the surrounding environment by discharging excess nutrients. This study used algae turf scrubber …

Detection And Observed Correlations Of Statin And Beta-Blocking Pharmaceuticals In West Virginia Surface Waters And Ichthyofauna, Joseph W. Kingsbury

Graduate Theses, Dissertations, and Problem Reports (ETD)

Within this thesis the source, presence, and the estimated ecotoxicological effects of cardiovascular medicines, specifically statins and beta-blockers are described within the Buckhannon, Tygart Valley, and West Fork Rivers of West Virginia. Included within this thesis is a general introduction providing key background information and relevant research related to the source, presence, and known toxicity of statins and beta-blockers. Following the general introduction there are 3 distinct chapters. Chapter 1 focuses on the estimated demographic based source loadings and the surface water concentration characteristics of statins and beta-blockers within the three rivers. Chapter 2 investigates the effects of statins and …

A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir

Turkish Journal of Veterinary & Animal Sciences

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative and quantitative features of native cattle and buffalo ovaries and oocytes to understand them comprehensively. In addition, it was conducted to pick the ideal ovaries for oocyte collection and to assess either maturation affects oocyte diameter or not. The ovaries were collected from the local slaughterhouses of Dhaka city, Bangladesh. Next, the quantitative parameters of ovaries were measured, the follicular fluid was aspired, the oocytes were classified, and the diameters of oocytes were determined in both pre and postmaturation. Here, the results …

The Effects Of Warming Rate On The Thermal Tolerance Of A Southern Population Of Striped Bass (Morone Saxatilis), James Barrett

Honors College Theses

Climate change and global warming is an ever-growing concern for our environment and the survivability of the animals which inhabit it. With water temperatures expected to rise 2-3℃ in the next century, many aquatic organisms may be limited in their habitats based on their thermal tolerance (Lleras 2019). Southern populations of striped bass (Morone saxatilis) in the Southeastern United States could be some of the hardest hit by the effects of climate change due to their unusual life history as compared to the more northern populations of striped bass. These southern striped bass do not engage in coastal …

Cutaneous Microbiome Of Red Fox (Vulpes Vulpes) Infected With Sarcoptic Mange (Sarcoptes Scabiei), Jacqueline C. Robidoux

Honors Theses and Capstones

Sarcoptic mange is a parasitic skin disease that affects countless mammals worldwide, including red foxes (Vulpes vulpes). The symptoms, such as hair loss, crusting, and the number of mites, vary between individual foxes. Sarcoptic mange damages the skin barrier, which in turn disrupts the biodiversity of bacteria in the microbiome. It is unknown what the biodiversity of bacteria is at each stage of the disease. This experiment will compare the microbiomes of different samples with and without mange; in hopes to reveal a connection between the different severities of sarcoptic mange and the biodiversity of bacteria and fungi …

Marine Heatwaves Modulate The Genotypic And Physiological Responses Of Reef-Building Corals To Subsequent Heat Stress, Kristen T. Brown, Amatzia Genin, Matheus A. Mello-Athayde, Ellie Bergstrom, Adriana Campili, Aaron Chai, Sophie G. Dove, Maureen Ho, Devin Rowell, Eugenia M. Sampayo, Veronica Z. Radice

Biological Sciences Faculty Publications

Back-to-back marine heatwaves in 2016 and 2017 resulted in severe coral bleaching and mortality across the Great Barrier Reef (GBR). Encouragingly, some corals that survived these events exhibit increased bleaching resistance and may represent thermally tolerant populations that can better cope with ocean warming. Using the GBR as a natural laboratory, we investigated whether a history of minimal (Heron Island) or severe (Lizard Island) coral bleaching in 2016 and 2017 equates to stress tolerance in a successive heatwave (2020). We examined the genetic diversity, physiological performance, and trophic plasticity of juvenile (<10 cm) and adult (>25 cm) corals of two common genera ( …

Of Dogs And Bonds, Jeffrey R. Stevens

Department of Psychology: Faculty Publications

In four of Charles Darwin’s classic works (Darwin, 1859, 1868, 1871, 1872), the domestic dog (Canis familiaris or Canis lupus familiaris)¹ featured prominently, offering key examples to illustrate his ideas about evolution, domestication, comparative behavior and cognition, and emotional expression. Darwin held a clear fondness for dogs, and when replying to letters detailing the “sagacity” of dogs, he remarked “I can believe almost anything about them” (Darwin, 2014). Despite the early interest in their behavior and cognition by a number of leading scientists, dogs were rarely subject to serious investigation as a study species for 150 years. …