Digital Commons Network^™

Imidacloprid Effects On The Survival And Cutaneous Microbiome Of Northern Leopard Frogs, Evelyn Margaret Osterloo

Honors Thesis

Amphibians are experiencing population declines due to habitat loss, climate change, and diseases, particularly chytridiomycosis caused by the fungus Batrachochytrium dendrobatidis. Protection from this fungal infection stems from the cutaneous microbiome. Pesticides have been shown to affect amphibians in a variety of ways, including reducing survival, locomotor performance, and accumulation in body tissues. Pesticides may also lead to changes in the cutaneous microbiome affecting amphibians who rely on these organisms as an anti-Bd defense mechanism. To investigate the effect of imidacloprid, a neonicotinoid insecticide, Northern Leopard frogs (Lithobates pipiens) were subjected to sublethal concentrations (control = …

Growing South Dakota (Spring 2026), College Of Agriculture, Food, And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 National heritage, statewide research, local impact
[Page] 7 About the South Dakota Agricultural Experiment Stations
[Page] 14 Managing the mooo-ment: How SDSU researchers are looking to improve cow health during the Transition Period
[Page] 18 Discovering strength in science: How education through undergraduate research prepares students for the future
[Page] 20 From India to Brookings: One grad student’s path to make a world of difference in virology
[Page] 22 Solving a billion-dollar problem: Identifying disease-resistent wheat genes
[Page] 24 Unlocking manure’s potential: SDSU leads multistate study on the power of swine manure in growing better crops
[Page] 28 …

Swimming Behavior Of Longfin Squid Doryteuthis Pealeii Paralarvae: Escape Jets To Pirouettes, Mel Lhuillier

Biological Sciences Theses & Dissertations

Upon hatching, squid paralarvae must become proficient at locomotion and prey capture before their yolk reserves are depleted, while also contending with the physical constraints associated with low/intermediate Reynolds number (Re) environments. Although some aspects of behavior and jet dynamics have been examined in squids during early ontogeny, little is known about routine behaviors in paralarvae, whether repeatable patterns are employed, or how these behaviors are achieved dynamically. To better understand swimming behaviors and the propulsive flows that drive them in paralarvae, high-speed video data and digital particle image velocimetry (DPIV) data were collected simultaneously from longfin squid Doryteuthis pealeii …

The Missing Lynx: Investigating Passive And Active Rewilding With The Eurasian Lynx, Isaiah Charles, Tatiana Flores, Isabella Lopez

Conservation Biology

The Eurasian Lynx (Lynx lynx) is a wild cat native to the boreal forests, mountains and grasslands of Europe and Asia (Bhattacharyya, 2025). The Eurasian lynx keep ungulate populations in check which allow for forests to recover from overgrazing, promoting biodiversity and ecological balance. However, the Eurasian lynx had become locally extinct across Europe by the 1900s due to habitat loss, hunting by farmers in retaliation for predation of livestock and the illegal fur trade. Conservation and ecological restoration efforts to rebuild the lynx population have been underway. Rewilding is a form of ecological restoration involving the reintroduction of a …

The Importance Of Body Size And Spatial Scale In Modeling Winter Bird Distributions, Carissa M. Mobley

Student Publications

Birds’ annual cycles include biologically linked breeding and non-breeding seasons, yet most research and bird atlases focus exclusively on the breeding season. There have been very few winter bird atlas projects in North America, and none have been published for the northeastern United States. Short daylight hours and harsh weather limit winter survey coverage, making species distribution models (SDMs) essential for addressing spatial gaps.

I evaluated the optimal spatial scale for SDMs across bird species differing in body size and tested whether optimal scale varies with body mass. Using 16,967 eBird checklists collected during the first winter survey period (December …

Mapping Sensory Landscapes: Distribution And Arrangement Of The Anterior Lateral Line System In Texas Catfish, Tatiana K. Flores, Ruben Tovar

Posters - 2026

Fish have a system of sensory organs known as the lateral line system. This system contains numerous mechanoreceptive neuromasts and electroreceptive ampullary organs (Northcutt et al, 2000). Neuromasts are small, hair-like structures located within canals in the lateral line system just under the fish’s skin and are open to the environment through small pores visible on its skin (Parker, 2025). Water flows into these canals through the pores which stimulates the sensitive hair cells, resulting in electrical signals sent to the fish’s brain. The brain interprets these signals and creates “images” of the fish’s surroundings, helping it to see even …

Muscular Degeneration In Caenorhabditis Elegans By Microplastic And Chemical Exposure Through Locomotion Analysis, Juan Pablo Olvera Rodriguez, Angelica Cue-Leon, David Mares

Posters - 2026

We use Caenorhabditis elegans (C. elegans) as a model organism to investigate the effects of microplastic and chemical exposure on muscle structure and function.

• Microplastics (MP) are pieces of plastic less than 5 μm, and Nanoplastics (NP) are less than 1 μm. Microplastics are man-made pollutants and have been found in the environment and in the food and water sources, including drinks from plastic bottles (Hu, 2020). • Our work relates environmental contamination to potential biological consequences at the muscular level, providing insight into how pollutants may contribute to degenerative damage.

• Polystyrene (PS) MPs alone and combined with …

Bird Population Response To Abiotic And Biotic Stresses In Urban & Restored Oak Savannah Habitat, Annabelle Ball

Posters - 2026

Birds are heavily impacted by urban development. Many experience habitat fragmentation and removal of food sources. However, other species can adapt to and even thrive in urban environments, thanks to certain tree species, bird feeders, and buildings. In North America, the house sparrow and the European starling are invasive species that benefit from urban environments (Bartelt, 2011; Fitzwater, 2014). Meanwhile, many habitats across the continent are experiencing restoration/rewilding efforts for the benefit of birds. One such habitat is the oak-savannah, which is characterized by 10-30% canopy cover and a mixture of prairie & woodland vegetation, supporting a high diversity of …

The Effects Human Presence Has On Dolphins Within A Natural Lagoon Enclosure In Managed Care, Mario Martinez, Winter Saldaña

Posters - 2026

● Previous research looked into the overall behavior of dolphins in managed care using observations of location (Dudzinski & Hill, 2025).

● This study provides opportunities to investigate the effects of human presence on dolphin activity and space use (Lauderdale et al., 2022; Trone et al., 2005).

● Purpose - To determine if human presence affects dolphin behavior / spatial use of natural lagoon.

● H1: Dolphin clustering will be seen in the most Northern part of the lagoon and area associated with humans consistently (Melillo et al., 2009).

● H2: Higher activity levels will be observed during the “during” …

Exploratory Evaluation Of Handler Performance And Processing-Related Outcomes During Cattle Handling, Morgan Marquardt

Department of Animal Science: Dissertations, Theses, and Student Research

Animals have several critical roles in society, including the production of food and fiber, and companionship. Contact with animals has been shown to benefit human physical and mental health, yet little research has investigated the impact that humans have on animals. Handler skill may considerably affect the extent to which humans influence animal health and wellbeing, and this may be a critical consideration for animal agriculture. Objective assessment of cattle handler skill has received limited attention in the scientific literature, despite its importance to human safety and animal welfare. When compared to less skilled handlers, highly skilled cattle handlers may …

Fisheries Research Report No. 358 - Recreational Fishing For Blue Swimmer Crab In Cockburn Sound: Estimates Of Effort And Catch In 2024/25, Alissa C. Tate, Claire B. Smallwood, Eva K M Lai

Fisheries Research Reports

The Cockburn Sound Blue Swimmer Crab (Portunus armatus) fishery was reopened to recreational fishing in December 2024 following a 10-year closure. Monitoring was essential to assess fishing effort and catch for this species.

A boat ramp (bus route) survey at four key access points (Leeuwin, Woodman Point, Palm Beach, and Point Peron) and shore-based roving creel survey (Fremantle South Mole to Point Peron) were used to collect data from December 2024 to May 2025. These surveys provide estimates of fishing effort, total catch (kept and released), harvest and catch rates for Blue Swimmer Crab in Cockburn Sound.

A total of …

Factors Influencing Sex-Based Movement Patterns In The Banded Garden Spider, Argiope Trifasciata (Forskal, 1776), Brandon R. Marrone

School of Biological Sciences: Dissertations, Theses, and Student Research

Movement is a fundamental ecological process shaping how individuals acquire mates, secure resources, and navigate heterogeneous landscapes. In species with extreme sexual size dimorphism (eSSD), such as the orb‑weaving spider Argiope trifasciata, males and females are predicted to experience different movement pressures driven by their reproductive roles. This thesis examines how individual traits and environmental conditions jointly influence movement across both sexes. Across two field seasons, we used intensive mark–recapture methods to quantify natural movement patterns of males and females within mapped prairie plots. The first chapter tested long‑standing predictions gravity hypothesis, which posits that smaller males benefit from …

Perceived Barriers To Integrated Crop–Livestock Systems: Evidence From A Nebraska Producer Survey, Omid Tarashtwal

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Integrated crop-livestock systems offer agronomic, economic, and environmental benefits, yet reintegration has been slow across the Northern Great Plains despite sustained interest. Existing research has documented barriers to transition conceptually, but quantitative evidence on which barriers producers perceive as most important and which producer and operation characteristics are associated with stronger barrier perceptions remains limited. This thesis addresses that gap using data from the 2023 Nebraska Agriculture Operator Survey.

The analysis focuses on 241 Nebraska producers, including 179 crop-only and 62 livestock-only operations. Seven models are estimated, combining ordered logit estimation for single-item barriers with linear regression for averaged barrier …

Do Young Belugas Play More Or Less Than Adult Belugas?, Winter Saldana

Posters - 2026

★ The presence of play is used as an indicator of well-being in many species including belugas (Hill et al., 2017; Hill & Ramirez, 2014). ★ Adult and young belugas engage in similar types of play, but the frequency with which belugas play with objects, the actions used during those object play bouts, and the type of materials the objects consist of have not been examined in detail (Hill & Ramirez, 2014). ★ The purpose of this study was to explore if object play exhibited by belugas varied in frequency of play bouts, actions, and materials used by young and …

Comparative Efficacy Of Oral And Cloacal Administration Of Lactobacillus Probiotics And Postbiotics Against Campylobacter Jejuni Colonization In Broiler Chickens, Shreeya Sharma, Hosni Hassan, Khaled Abdelaziz

Publications

Campylobacter jejuni remains a major cause of foodborne illness worldwide, with poultry serving as the primary reservoir. In the absence of commercial vaccines or effective feed additives, probiotics and their byproducts (postbiotics) represent a promising and sustainable approach to reducing Campylobacter colonization in poultry. This study compared the efficacy of oral and cloacal administration of probiotic lactobacilli and their postbiotics in reducing Campylobacter colonization and modulating the cecal microbiome in broiler chickens. Day-old chicks were assigned to seven treatment groups that received either probiotics (live cells of four poultry-derived Lactobacillus strains: L. reuteri P43, L. acidophilus P42, L. animalis P38, …

Teeth That Tell Time: Elemental Analysis From Shark Teeth, Gisele-Elizabeth Perez, David Turner

Research Papers

Sharks (Chondrichthyes) have hunted in the oceans for over 400 million years, their teeth leaving history behind, constantly becoming a part of the fossilization process (Worm et al. 2013). Their survival throughout 5 major extinctions is due to their ability to adapt easily with slow metabolism, a wide range of diet, and an efficient physiology. Shark teeth are vital to the hunting and consumption of their prey, making it a point for scientists to study them. Their tooth minerals contain fluorapatite in their enameloid, and dentin in the “root” of the tooth, with extinct species containing dentin and hydroxyapatite in …

Teeth That Tell Time: Elemental Analysis From Shark Teeth, Gisele-Elizabeth Perez

Posters - 2026

Sharks (Chondrichthyes) have hunted in the oceans for over 400 million years, their teeth leaving history behind, constantly becoming a part of the fossilization process (Worm et al. 2013). Their survival throughout 5 major extinctions is due to their ability to adapt easily with slow metabolism, a wide range of diet, and an efficient physiology. Shark teeth are vital to the hunting and consumption of their prey, making it a point for scientists to study them. Their tooth minerals contain fluorapatite in their enameloid, and dentin in the “root” of the tooth, with extinct species containing dentin and hydroxyapatite in …

Mapping Sensory Landscapes: Distribution And Arrangement Of The Anterior Lateral Line System In Texas Catfish, Tatiana Flores, Ruben Tovar

Research Papers

Fish have a system of sensory organs known as the lateral line system. This system contains numerous mechanoreceptive neuromasts and electroreceptive ampullary organs (Northcutt et al, 2000). Neuromasts are small, hair-like structures located within canals in the lateral line system just under the fish’s skin and are open to the environment through small pores visible on its skin (Parker, 2025). Water flows into these canals through the pores which stimulates the sensitive hair cells, resulting in electrical signals sent to the fish’s brain. The brain interprets these signals and creates “images” of the fish’s surroundings, helping it to see even …

Ecological Studies Of Wolves On Isle Royale, Sarah R. Hoy, Rolf O. Peterson, John A. Vucetich

Ecological Studies of Wolves on Isle Royale

Annual Report 2025-2026

Comparing Head Shape Of Polymorphic Subterranean E. Latitans And Subterranean E. Sp. 4, Brandon Chapa, Jordan Cosgrove, Lizzeth Holguin

Posters - 2026

Eurycea latitans (E. latitans) from Honey Creek Cave and unnamed Eurycea species from New Braunfels, Texas (E. sp. 4) are two paedomorphic subterranean species. E. latitans is polymorphic, meaning it also exists as a surface species. Similarities in morphology of subterranean salamander species may be due to convergent evolution as a result of living in similar environments. These similarities include having flatter, wider heads, and wider mouths than surface species (Tovar et al. 2025). Variation may also exist among different subterranean salamander species. Texas Eurycea salamanders display “complex patterns of morphological divergence”, suggesting that isolation and local environmental pressures can …

Beluga Buddies: The Preferred Social Network Of Belugas In Managed Care, Valery Marinelarena, Diamond Garcia

Posters - 2026

Belugas societies are not fixed  and group composition is everchanging. Group composition can also influence behavior. This study aims to examine the preferred social networks and the role age plays in the behavioral diversity within a group of managed care belugas housed at Sea World San Antonio. Results Supported TYO had a preferred, non-random social network and ATL performing more chinslaps than TYO.

Emerald Bowfin Amia Ocellicauda Ecology In Three Minnesota Lakes: Abundance, Age Analysis, Diet Composition, And Stable Isotope Food Web, Griffin Blegen

Biology Graduate Theses

The Emerald Bowfin Amia ocellicauda is a predatory fish species native to Minnesota that has often been discriminated against. This research expands ecological knowledge of Bowfin in Minnesota and provides population data to guide future management. Bowfin were sampled in the spring of 2024 and 2025 and a multi-census mark–recapture model was used to estimate abundance in three lakes. Estimated populations were 196 individuals in Little Toad Lake (95% CI: 116, 242) or 4.4 fish/littoral ha (95% CI: 2.7,5.7), 657 in Lake Sarah (95% CI: 436, 1014) or 4.2 fish/littoral ha (95% CI: 3.0, 6.7), and 612 in Gun Lake …

Just Unmasking Knowledge? A Critical Interpretation Of Fluctuating Jellyfish Populations For A Mass Media Audience, Wrenne R. Grone

Undergraduate Honors Thesis Projects

While podcasts may not be the most popular form of mass media, they are growing in their accessibility and prevalence in educational discourse. My creative work, in the form of a podcast, unmasks scientific knowledge in an untraditional, mass-media form and will use podcast techniques, such as music transitions and storytelling techniques, to maintain engagement. Additionally, my podcast aims to transpose academic knowledge into a comprehensible, interesting form for a non-academic audience. Jellyfish have no brains, no heart, no blood, and no bones and yet, they have survived for millions of years. The goal of my podcast is to educate …

Polyhydroxybutyrate (Phb) From Biofloc Systems Enhances The Growth And Immune Performance Of Genetically Improved Farmed Tilapia, Menaga Meenakshisundaram, Jimmy B. Mboya, Felix Sugantham

All Peer-Reviewed Publications

This study characterised polyhydroxybutyrate (PHB)-producing bacteria from the culture water of genetically improved farmed tilapia (GIFT) in biofloc ponds and investigated the influence of PHB on the growth and immune performance of GIFT. Out of 40 bacterial isolates, Bacillus infantis, Exiguobacterium profundum, Bacillus subtilis, Bacillus megaterium, Klebsiella pneumoniae and Enterococcus hirae showed positive results for PHB against fluorescent stains. The PHB yield in the range of 5%–95.45% (w/w) was recorded, with the highest from K. pneumoniae (95.45% w/w). The experimental treatments included a basal diet as a control (C), a basal diet with PHB (5 g kg⁻¹) from …

Risk Factors Of Leptospirosis Among Agricultural Workers In Southeast Asia: Literature Review, Ronggo Adi Wibowo, Wawa Najwa Nurzakia, Meirany Sasitha Kirana, Yulia Arthemisia Wijaya, Jasmine Ayumaharani Sekaryudha, Dwi Sarwani Sri Rejeki, Rosita Dwi Jayanti

Jurnal Epidemiologi Kesehatan Indonesia

The agricultural sector is the primary source of livelihood for communities in Southeast Asia; however, these activities increase the risk of exposure to zoonotic diseases, particularly leptospirosis. Southeast Asia is one of the regions with the highest incidence of leptospirosis in the world. This literature review aims to identify risk factors for leptospirosis among agricultural workers in Southeast Asia. The search for research articles utilized three databases (Google Scholar, PubMed, and ScienceDirect) using a descriptive approach based on literature review and the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A total of 9,800 articles were screened for …

Evaluation Of Pinhole Castration Techniques Compare With Traditional Method For Castration In Rabbits, Wakash Oad, Faiz Muhammad Khand, Habibullah Janyaro, Muhammad Mohsen Rahimoon, Muhammad Ramzan, Dilpat Rai Menghwar, Benazir Sahito

Journal of Bioresource Management

Castration is a routine surgical intervention used to sterilize animals. Among the different conventional methods, the pinhole castration provides offer significant advantages. This study was conducted on 12 healthy male domestic rabbits at Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, to evaluate the physiological impacts of traditional versus pinhole castration techniques. The rabbits were randomly divided into group A and group B with six rabbits per group. Key parameters included preoperative vital signs, intraoperative monitoring at 5, 10, and 15 minutes, intra- and postoperative complications, serum cortisol levels (preoperative, 24 hours, 5 and 10 days post-surgery) and …

A Comparative Evaluation Of Marinades' Effect On The Physical And Sensory Attributes Of Spent Hen Meat, Rashid Ali Korejo, Atta Hussain Shah, Muneer Ahmed Jamali, Tarique Ahmed Khokhar

Journal of Bioresource Management

The study focused on consumer reluctance to choose spent hen meat, an affordable and underutilized protein source, which is often rejected for its tough texture and reduced acceptability. Therefore, the research aimed to assess the effectiveness of natural marinades in enhancing the physical and sensory qualities of spent hen meat, thereby improving its consumer appeal and market potential. Meat samples were allocated into seven groups: an untreated control and six treatments comprising yogurt, ginger extract, tamarind juice, and honey. Fresh meat exhibited an initial pH of 6.21 and a Water-Holding Capacity (WHC) of 34.84 %, Cooking Loss (CL) of 32.75 …

Comparative Assessment Of Growth And Morphometric Characteristics In Naked Neck, Rhode Island Red, And Black Australorp Chickens, Naseer Ahmad, Sarzamin Khan, Rafi Ullah, Kamran Khan

Journal of Bioresource Management

This study investigated growth performance and morphometric characteristics in three chicken genotypes namely Naked Neck (NN), Rhode Island Red (RIR), and Black Australorp (BAL). One-hundred and eighty chicks (age, day-old), comprising 60 birds from each genotype, were distributed randomly into three replicates per group, with 20 chicks per replicate, under a completely randomized design. Birds were reared on deep litter for 18 weeks. Hatch weights were significantly higher (P < 0.05) in BAL (30.7 g) and RIR (30.2 g) compared to NN (28.2 g). Body weights showed no significant differences (P > 0.05) from weeks 2 to 4, but BAL and RIR outperformed NN (P < 0.05) from weeks 6 to 10. BAL maintained the highest body weight from weeks 12 to 18 (P < 0.05). Total weight gain (0-18 weeks) was significantly greater in BAL (1567 g), followed by RIR (1491 g) and NN (1219 g). The genotypes had significant effects on both feed intake and feed conversion ratio (FCR; P < 0.05). At 18 weeks of age, FCR was most efficient in BAL (3.53), followed by RIR (3.83), and NN (4.85). Mortality between weeks 4 and 18 was lowest in NN at 1.7 %, compared with 1.81 % in RIR and 1.84 % in BAL. Significant differences (P < 0.05) among genotypes were also observed in morphometric characters such as shank length, keel length, and breast circumference, with BAL generally showing larger measurements. In conclusion, BAL exhibited superior growth, feed efficiency, and morphometric characteristics, while NN demonstrated lower weight gain but better survivability, highlighting genotype-specific advantages for different production goals.

Dietary Oil From Hermetia Illucens L. Larvae Promotes Weight Gain And Alters Leukocyte Profiles In Mus Musculus L, Rudy Agung Nugroho Ran, Reni Kurniati, Widya Putri Ambar Sari, Reynaldo Imanuel Sompotan, Retno Aryani, Hetty Manurung, Rudianto Rudianto

Makara Journal of Science

The escalating global demand for high-quality animal-derived proteins has driven the search for sustainable alternatives. Black soldier fly larvae (BSFL; Hermetia illucens L.) oil presents a promising option, by addressing organic waste management while providing nutrient-rich feed. This study evaluated the effects of dietary supplementation with BSFL oil on growth performance, hematological characteristics, and blood lipid profiles in mice (Mus musculus). Male mice were randomly assigned to five groups (n = 5): positive control (fish oil, 5 mL/kg body weight [BW]), negative control (Aquadest, 5 mL/kg BW), and three treatment groups receiving BSFL oil at 2.5, 5, and …

Comparative Morphometric Study Of Saanen And Indigenous Goats, In Northern Pakistan, Nadar Khan, Ihsan Uddin, Sohail Khan, Hayat Ullah Khan, Muhammad Suleman

Journal of Bioresource Management

Goats play an essential role in sustaining rural communities in Pakistan by providing milk, meat, and income under diverse production systems. This study compared the morphometric features of exotic Saanen goats with local breeds (Beetal, Kaghani, Gaddi, and non-descriptive) in Swat, northern Khyber Pakhtunkhwa, to evaluate growth patterns, body structure, and the potential for body weight estimation. A total of 500 animals were sampled from research stations and smallholder farms, with morphometric and qualitative traits recorded following FAO guidelines. Distinct differences among breeds were observed. Beetal goats exhibited the highest body weight (58.2 ± 2.0 kg), chest girth, and body …