Digital Commons Network^™

Determination Of Antibiotic Resistance In Salmonella Typhimurium And Salmonella Kentucky Serotypes Of Animal Origin Using Conventional And Molecular Methods, Zeynep Şik, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

The high incidence of multidrug-resistant Salmonella Typhimurium and Salmonella Kentucky isolates is a concern for human and animal health. This study aimed to investigate the antibiotic resistance determinants of a total of 150 S. Typhimurium and S. Kentucky isolates obtained from cows, calves, lambs, and poultry. Salmonella isolates were tested against 13 different antimicrobials using the Kirby-Bauer disc diffusion method, and genotypic antimicrobial resistance determinants were investigated by polymerase chain reaction. Significant differences were detected among serovars for gentamicin, streptomycin, ampicillin, sulfonamide, nalidixic acid, ciprofloxacin, and tetracycline resistance, with the rates of resistance to these antibiotics being determined to be …

Honest Signalling Of Age In A Territorial Breeder, Brian A. Hoover, Walter H. Piper

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Age often indicates phenotypic quality, ecological experience or social status in animals. Consequently, behavioural signals in territorial animals that accurately convey age to potential mates or rivals might provide useful information for both signaller and receiver about the likelihood of aggression. We tested whether male territorial yodels convey information about age in the common loon, Gavia immer. Using a sample of male loons of known age, we found that the dominant frequency of Fintro3, a dominant syllable in the beginning sequence of a male yodel, increases strongly with age, but we were unable to replicate an earlier finding linking …

Revision Of The Genus Olivierus In Xinjiang, China, With Comments On Mesobuthus Thersites (Scorpiones: Buthidae), Victoria Tang, Zhenbang Liu, Matthew R. Graham, Victor Fet, František Kovařík, František Šťáhlavský

Euscorpius

The genus Olivierus Farzanpay, 1987 in Xinjiang Uygur Autonomous Region, China, is revised based on recently collected topotypes and other populations from 12 localities. Brief differential diagnoses are provided, with colored illustrations and photos in vivo habitus, emphasizing the key characters. Chinese appellations, conservation status, and documentation of behavior and post-envenomation symptoms are also included. Only two species are now recognized for this genus in Xinjiang: O. longichelus (Sun & Zhu, 2010) and O. przewalskii (Birula, 1897), based on both morphological and molecular evidence. The two species exhibit extensive distribution in Xinjiang (China) while also occurring in adjacent countries. Three …

Kentucky Warbler (Vol. 100, No.1), Department Of Library Special Collections

The Kentucky Warbler

No abstract provided.

Assessment Of The Status Of The Pilbara Demersal Scalefish Resource, Corey Wakefield, Ainslie Denham, Fabian Trinnie, Sybrand A. Hesp, Rachel Marks, Dion Boddington, Stephen J. Newman

Fisheries Research Reports

Executive Summary

For this assessment of the Pilbara Demersal Scalefish Resource (PDSR) of Western Australia, red emperor and bluespotted emperor are the indicator species used to represent the status of the resource. In addition, goldband snapper, though not an indicator species, is relatively abundant in the annual catches, particularly in deeper waters (around 100 m) and in the western Pilbara region and its status has also been assessed.

Long Term Sustainable Use Of Demersal Gillnet And Demersal Longline In Western Australia’S Temperate Shark Fisheries - Fisheries Research Report 340, Matias Braccini, Jack Parker, Daniela Waltrick, Maddison Watt, Nick Blay, Clinton Syers

Fisheries Research Reports

The Temperate Demersal Gillnet and Demersal Longline Fisheries (TDGDLF) are one of the main commercial shark fisheries in Australia. Most fishers in the TDGDLF use demersal gillnets to target sharks with scalefish taken in smaller amounts. Demersal longlines are also permitted but are not widely used. Demersal gillnets are size selective, but they do not necessarily discriminate among species, catching target and non-target species.

The Western Australian Fishing Industry Council (WAFIC) received funding as part of the Australian Government’s Our Marine Parks Grants Program to undertake research on the TDGDLF. This project was administered under the direction of a steering …

Fisheries Research Report No. 339: Assessment Of The Status Of The South Coast Demersal Scalefish Resource, Jeffrey V. Norriss, Sybrand A. Hesp, David V. Fairclough, Rubie Evans-Powell, B. Hancock, Emily A. Fisher, Tim Leary, Nick Jarvis

Fisheries Research Reports

This document provides the 2023 risk-based weight of evidence stock assessments for snapper, Bight redfish, hapuku, blue morwong, and Western blue groper for 2023 in the South Coast Bioregion (SCB).

Experimental Sars-Cov-2 Infection Of Elk And Mule Deer, Stephanie M. Porter, Airn E. Hartwig, Helle Bielefeldt-Ohmann, Jeffrey M. Marano, J. Jeffrey Root, Angela M. Bosco-Lauth

United States Department of Agriculture Wildlife Services: Staff Publications

To assess the susceptibility of elk (Cervus canadensis) and mule deer (Odocoileus hemionus) to SARS-CoV-2, we performed experimental infections in both species. Elk did not shed infectious virus but mounted low-level serologic responses. Mule deer shed and transmitted virus and mounted pronounced serologic responses and thus could play a role in SARS-CoV-2 epidemiology.

Birds, Bats And Minds. Tales Of A Revolutionary Scientist: Donald R. Griffin. Volume Three, Carolyn A. Ristau

eBooks

In this three-volume biography, we revisit the life and accomplishments of the revolutionary scientist, Donald R. Griffin. He encountered a lifetime of initial hostile resistance to his ideas and studies; now they are largely accepted. He and a colleague discovered the phenomenon of echolocation used by bats to navigate and capture insects, proposed that birds navigate guided by such cues as the sun and stars, and suggested that animals are likely aware, thinking and feeling beings. Forty interviews with his colleagues and friends help us understand the young emerging scientist and the mature researcher. We learn about his and others’ …

Preferential Binding Of Polyphenols In Blackcurrant Extracts With Milk Proteins And The Effects On The Bioaccessibility And Antioxidant Activity Of Polyphenols, Ting Mao, Fnu Akshit, Iresha Matiwalage, Subha Sasidharan, Caren Meyn Alvarez, Philip Wescombe, Maneesha S. Mohan

Dairy Science and Food Science Faculty Publications: 2022 to Present

Milk proteins are well-known delivery agents; however, there is no clear understanding of the competitive interactions of milk proteins with polyphenols in mixed complex systems. Here, we investigate the preferential competitive interactions of different polyphenols present in blackcurrant extract with milk proteins by quantifying the protein-bound polyphenols and comparing the factors affecting these interactions. In addition, bioaccessibility and antioxidant activity were studied after in vitro gastric digestion. Our results indicated that polyphenols from blackcurrant extracts were preferentially bound to caseins more than whey proteins, with noncovalent interactions causing secondary structural changes in the protein. The hydrophobicity and the charge of …

Can Moderate-Quality Bermudagrass Baleage Be Used To Stretch Wheat Pasture?, P. A. Beck, M. R. Beck, D. Hubbell Iii, T. Hess, A. P. Foote, M. S. Gadberry, Beth Kegley

Animal Science Faculty Publications and Presentations

Objectives

Materials and Methods

Effects Of Environmental Enrichments On Welfare And Hepatic Metabolic Regulation Of Broiler Chickens, Seong W. Kang, Karen D. Christensen, Michael T. Kidd, Sara K. Orlowski

Poultry Science Faculty Publications and Presentations

The aims of this study were to find suitable environmental enrichment (EE) and evaluate the combined effect of two EEs, variable light intensity (VL) lighting program and EH, on mental health and hepatic metabolic regulation in commercial broilers. To find the advantageous EEs for broilers, three different EEs (board, hut, and ramp) were tested in trial 1. EEs were placed and the engagement of birds to EEs, dustbathing behavior, and daily physical activity were observed. Birds treated with huts showed higher engagement than the board- or ramp-treated birds (p < 0.05). The results of dustbathing behavior and daily physical activity indicated that the environmental hut (EH) is the most favorable enrichment for broilers. In the second trial, to test the effect of EHs on mental health and hepatic metabolic conditions, the brain and liver were sampled from the four treatment birds (20 lx_Con, 20 lx_Hut, VL_Con and VL_Hut) on day 42. The lower expression of TPH2 (tryptophan hydroxylase 2) of VL_Hut birds than those of VL_Con and 20 lx_Hut treated birds suggests the combining effect of EHs with the VL lighting program on the central serotonergic homeostasis of broilers. Reduced expressions of TH (tyrosine hydroxylase), GR (glucocorticoid receptor), BDNF (brain-derived neurotrophic factor) of VL_Hut treated birds compared to those of VL_Con and 20 lx_Hut birds suggest lower stress, stress susceptibility, and chronic social stress in VL_Hut treated birds. The expression of CPT1A (carnitine palmitoyl transferase 1) increased over three-fold in the liver of VL_Con birds compared to 20 lx_Con birds (p < 0.05). EHs treatment in VL birds (VL_Hut) significantly decreased CPT1A but not in 20 lx birds (20 lx_Hut). The expression of ACCα (acetyl-CoA carboxylase alpha) was significantly decreased in VL_Con birds compared to 20 lx_Con birds. There was no significant difference in the hepatic FBPase (fructose-1,6-bisphosphatase), GR, and 11β-HSD1 (11 β-hydroxysteroid dehydrogenease-1) expression between 20 lx_Con and VL_Con birds, but EHs significantly stimulated GR in 20 lx_Hut birds, and stimulated FBPase and 11β-HSD1 expression in the VL_Hut birds compared to 20 lx_Con birds, suggesting that the VL lighting program reduced fatty acid synthesis and increased fatty acid β-oxidation in the broilers’ liver and VL_Hut improved the hepatic de novo glucose production. Taken together, the results suggest that the stimulated voluntary activity by EHs in the light-enriched broiler house improved mental health and hepatic metabolic function of broilers and may indicate that the improved hepatic metabolic function contributes to efficient nutritional support for broilers.

Avian Symbionts Of Sulawesi: Identifying And Correlating Malaria Parasites And The Microbiome In Communities Of Endemic Avian Hosts, Rachael Joakim

Dissertations, Theses, and Capstone Projects

Since the evolution of complex organisms, there have been communities of symbionts inhabiting them. Thanks to the development of parallel sequencing techniques, DNA of entire microbial communities can be sequenced from a single sample. Some microbes can be parasitic in nature, such as Haemosporida, an order of eukaryotes causing malaria. While most symbionts form a more commensal host relationship in wild systems, host-symbiont dynamics can vary in pathogenicity based on biotic and abiotic factors. In vertebrates, microbial communities in the gut play a major role in host function, including immune responses to Haemosporida. Though this relationship is most commonly explored …

Data Supporting The Journal Article: Aquatic Invasive Species Prevention In The Live Baitfish Trade: The Role Of Regulations, Education, And Inspections, Hannah Mulligan, Mark Kaemingk, Alison A. Coulter

NRM Departmental Data Sets

Data summery: Calculated values for regulations, education, and inspections that were compared among regions to determine focus on each preventative action.

File type: .xlxs
File size: 57kb

A Comprehensive Analysis Of Antibiotic Resistance Genes In The Giant Panda Gut, Feilong Deng, Yanhua Han, Yushan Huang, Desheng Li, Jianmin Chai, Linhua Deng, Ming Wei, Kai Wu, Huabin Zhao, Guan Yang, Jiangchao Zhao, Ying Li, Chengdong Wang

Animal Science Faculty Publications and Presentations

In this study, we have successfully constructed a comprehensive database of metagenome-assembled genomes (MAGs) pertaining to the gut microbiota of the giant panda. Through our analysis, we have identified significant reservoirs of antibiotic resistance genes (ARGs), namely Escherichia coli, Citrobacter portucalensis, and Klebsiella pneumoniae. Furthermore, we have elucidated the primary contributors to ARGs, including Streptococcus alactolyticus and Clostridium SGBP116, in both captive and wild pandas. Additionally, our findings have demonstrated a higher prevalence of ARGs in the metagenome, with notable expression of the RPOB2 gene in S. alactolyticus. Crucially, 1217 ARGs shared homology with human gut …

Clostridium Butyricum And Carbohydrate Active Enzymes Contribute To The Reduced Fat Deposition In Pigs, Lingyan Ma, Shiyu Tao, Tongxing Song, Wentao Lyu, Ying Li, Wen Wang, Qicheng Shen, Yan Ni, Jiang Zhu, Jiangchao Zhao, Hua Yang, Yingping Xiao

Animal Science Faculty Publications and Presentations

Pig gastrointestinal tracts harbor a heterogeneous and dynamic ecosystem populated with trillions of microbes, enhancing the ability of the host to harvest energy from dietary carbohydrates and contributing to host adipogenesis and fatness. However, the microbial community structure and related mechanisms responsible for the differences between the fatty phenotypes and the lean phenotypes of the pigs remained to be comprehensively elucidated. Herein, we first found significant differences in microbial composition and potential functional capacity among different gut locations in Jinhua pigs with distinct fatness phenotypes. Second, we identified that Jinhua pigs with lower fatness exhibited higher levels of short-chain fatty …

Therapeutic Potential Of Snake Venom: Toxin Distribution And Opportunities In Deep Learning For Novel Drug Discovery, Anas Bedraoui, Montamas Suntravat, Salim El Mejjad, Salwa Enezari, Naoual Oukkache, Elda E. Sanchez, Jacob Galan, Rachid El Fatimy, Tariq Daouda

School of Medicine Publications

Snake venom is a rich source of bioactive molecules that hold great promise for therapeutic applications. These molecules can be broadly classified into enzymes and non-enzymes, each showcasing unique medicinal properties. Noteworthy compounds such as Bradykinin Potentiating Peptides (BPP) and Three-Finger Toxins (3FTx) are showing therapeutic potential in areas like cardiovascular diseases (CVDs) and pain-relief. Meanwhile, components like snake venom metalloproteinases (SVMP), L-amino acid oxidases (LAAO), and Phospholipase A2s (PLA2) are paving new ways in oncology treatments. The full medicinal scope of these toxins is still emerging. In this review, we discuss drugs derived from snake venoms that address …

Antimicrobial Tolerance In Salmonella: Contributions To Survival And Persistence In Processing Environments, Tomi Obe, Aaron S. Kiess, Ramakrishna Nannapaneni

Poultry Science Faculty Publications and Presentations

Salmonella remains a top bacterial pathogen implicated in several food-borne outbreaks, despite the use of antimicrobials and sanitizers during production and processing. While these chemicals have been effective, Salmonella has shown the ability to survive and persist in poultry processing environments. This can be credited to its microbial ability to adapt and develop/acquire tolerance and/or resistance to different antimicrobial agents including oxidizers, acids (organic and inorganic), phenols, and surfactants. Moreover, there are several factors in processing environments that can limit the efficacy of these antimicrobials, thus allowing survival and persistence. This mini-review examines the antimicrobial activity of common disinfectants/sanitizers used …

Effects Of Beauveria Bassiana On The Mortality And Thorax Width Of Bombus Impatiens Colonies, Bri Petty

Undergraduate Research Symposium

Pesticides are widely used to protect against unwanted insects, but risk assessments of the effects on pollinators have traditionally only been applied on an individual level, not a population level. This has allowed many pesticides to be approved without the knowledge of sub-lethal effects, such as life history characteristics and population structure to name a few. The biopesticide BotaniGard, containing the entomopathogenic fungus Beauveria bassiana, has previously been thought to be safe for non-targeted insects, but recent studies have found it to have significant negative effects on pollinators. Such negative effects can include decrease in longevity and odorant responsiveness with …

Animal Conservation In St. Louis, Kate O'Sullivan

Undergraduate Research Symposium

St. Louis has a multitude of organizations involved in the natural sciences. But how many of them actually contribute towards animal conservation? The St. Louis Zoo is an organization that focuses a lot of its effort on presentation, so how does that impact the funds that go towards actually saving the animals? I plan to dive into the different animal-based organizations in St. Louis and its surrounding areas, as well as discuss the positives and negatives of each organization. Furthermore, I will provide examples from several sources that I have been reviewing all year to support my claims. I plan …

Surveys Of Aphonopelma Hentzi In Missouri: Conservation Efforts Through Population, Genetics, And Habitat Studies, Anderson B. Spencer Mr., Becky Hansis-O'Niell

Undergraduate Research Symposium

Aphonopelma hentzi and other tarantula species are greatly understudied organisms. In the Missouri regions there is little knowledge on their mating patterns, gene diversity, or population sizes. The focal glades in this experiment display regional fragmentation, which could prevent tarantulas from traveling and mating between regions of their glades. Due to the lack of knowledge surrounding them, methods for determining gene diversity are harmful to the organism. Our recent work has shown that it is possible to extract genetic information from the molts of burrowing tarantulas. This will allow us to safely determine the lineages and interbreeding patterns of the …

Safety And Health Management Planning For Veterinarians, Jessie Salter, Michael Pate, Kerry A. Rood

All Current Publications

Veterinary staff health and safety are of primary importance to the profession. Although this is inherently understood, oftentimes, staff safety is not given the time and resources needed to promote a safe work environment free from unnecessary risks or exposures. This handbook educates clinic leaders on valuing and creating a safety culture in practice as well as implementing appropriate safety policies and procedures. Veterinary personnel are more likely to experience a work-related injury than policemen, firefighters, and construction workers. These statistics illustrate the need for a stronger safety culture in veterinary medicine. This handbook provides an outline on how to …

Every Little Thing, Dereck Daschke

Journal of Religion & Film

This is a film review of Every LIttle Thing (2024), directed by Sally Aitken.

Genetic Background Of Juniper (Juniperus Spp.) Consumption Predicted By Fecal Near-Infrared Spectroscopy In Divergently Selected Goats Raised In Harsh Rangeland Environments, Henrique A. Mulim, John W. Walker, Daniel F. Waldron, Danilo G. Quadros, Lorena F. Benfica, Felipe E. De Carvalho, Luiz F. Brito

Purdue University Libraries Open Access Publishing Fund

Background

Junipers (Juniperus spp.) are woody native, invasive plants that have caused encroachment problems in the U.S. western rangelands, decreasing forage productivity and biodiversity. A potential solution to this issue is using goats in targeted grazing programs. However, junipers, which grow in dry and harsh environmental conditions, use chemical defense mechanisms to deter herbivores. Therefore, genetically selecting goats for increased juniper consumption is of great interest for regenerative rangeland management. In this context, the primary objectives of this study were to: 1) estimate variance components and genetic parameters for predicted juniper consumption in divergently selected Angora (ANG) and composite Boer …

Parental Environnement Influences Offspring Gene Expression In The Olympia Oyster, Ostrea Lurida., Mark S. Yeats

LSU Master's Theses

The Olympia Oyster is the only native oyster species on the Pacific coast of North America. Seasonality in water temperatures across this threatened species’ broad and fragmented range has been linked to its development, reproductive timing, reproductive activity, growth, and survival rates. Recent evidence suggests parental environments can have carry-over effects on larval growth and respiration rates in response to temperature. In this study, we utilized transcriptomic methods to identify the underlying mechanisms of this cross-generation plasticity. Sequence libraries of adult ctenidia tissue and pooled larval replicates were extracted and spawned from hatchery raised oysters of the same parentage immediately …

Colloidal Calcium Phosphate (Ccp) Causes Problematic Acid Variation In Cheddar Cheese, Sakshi Singh

Research on Capitol Hill

pH variation in cheese during the early ripening stages causes changes in the texture, body, and flavor of cheese. The dairy industry, encompassing over 50,000 dairy farms nationwide and several regional dairy farmers and companies in Utah, suffers substantial financial loss as cheese with varying pH is rendered mostly useless.

Attitudes Of South-Central Kentucky Residents Towards Coyotes And Their Management, Andrew T. Mcqueen, Stephen Sumithran Phd

Posters-at-the-Capitol

The existence of the coyote in the eastern U.S. is a recent development and the public’s perception of coyotes has been widely studied in urban areas. However, perceptions in rural areas, especially rural Appalachia, have been largely unstudied. It is necessary for wildlife managers to understand the public’s perceptions of coyotes and coyote management when creating management plans. We conducted a public survey in South-central Kentucky to determine perceptions toward coyotes and coyote management methods. The survey contained questions about demographics (age, gender, county of residence, landownership, etc.), perceptions (fears, opinions), knowledge (true/false questions), and preferred management practices (hunting, trapping, …

Program Requirements For Beef Cattle Certified As Usda Organic, Carsten Loseke, Elliott James Dennis

Cornhusker Economics

This document provides an overview of the use and production of the USDA Certified Organic program for the beef cattle industry, with an emphasis on the state of Nebraska. We detail what cattle qualify, feed requirements, medical and health standards of cattle, and premiums paid by consumers for USDA certified organic products. All information is taken from the Organic Foods Production Act Provisions available in the Federal Register.

Puerulus Settlement Forecasting In A Harvest Strategy Evaluation Of The Rock Lobster Fishery In South Australia, Richard Mcgarvey, Adrian Linnane, John E. Feenstra, Janet M. Matthews, Lachlan J. Mcleay, Annabel Jones, Kyriakos Toumazos, Simon De Lestang

Fisheries Research Articles

Harvest strategies are utilised in the management of fishery resources globally. Critical to their success is harvest strategy evaluation, whereby future performance is assessed through projection modelling. Using the observed relationship between a puerulus settlement index and model-estimated recruitment, we evaluated a southern rock lobster (Jasus edwardsii) harvest strategy in South Australia. Short-to-medium puerulus settlement recruitment forecasting was incorporated into future projections of catch, catch per unit effort, and percent unexploited egg production (%UEP) during 2019–2022. Observed values of indicators that closely aligned with projected outputs indicated that puerulus forecasting underpinned reliable evaluation of future fishery performance. A …

Effect Of Dietary Phytase On Water And Fecal Prokaryotic And Eukaryotic Microbiomes In A Hybrid Tilapia (Oreochromis Aureus X O. Niloticus) Mixotrophic Biofloc Production System, Candis L. Ray, Jason Abernathy, Bartholomew Green, Adam Rivers, Kevin Schrader, Steven Rawles, Matthew Mcentire, Miles Lange, Carl Webster

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study we evaluated feed and temporal effects on prokaryotic (16S) and eukaryotic (18S) microbiome dynamics in an outdoor mixotrophic biofloc system used to grow hybrid tilapia (Oreochromis aureus x O. niloticus) to market size. The dicalcium phosphate -free practical feed treated with 6-phytase (3000 FTU/kg; P-Free+ Phytase) and positive (Control) and negative (P-Free) control practical feeds used were formulated to contain 27.7% digestible protein and 4% lipid. Sex reversed, all-male hybrid tilapia (28.9 g/fish) were stocked (29 fish/m³) in 9 outdoor rectangular tanks (16.6 m³) and grown for 154 days. The …