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Glycine, Serine, And Arginine Additions To Cobb 500 Female Broilers Fed Dietary Variations In Crude Protein, M. A. Anas, N.S.B.M. Atapattu, K. B. Nelson, S. W. Crafton, Michael T. Kidd Sep 2024

Glycine, Serine, And Arginine Additions To Cobb 500 Female Broilers Fed Dietary Variations In Crude Protein, M. A. Anas, N.S.B.M. Atapattu, K. B. Nelson, S. W. Crafton, Michael T. Kidd

Poultry Science Faculty Publications and Presentations

This study investigated the effects of supplemental Gly, Ser, and Arg on growth performance, carcass traits, footpad dermatitis, and litter ammonia emission of broilers fed low-protein diets from 18 to 42 d of age. A total 576 one-day-old female Cobb 500 broilers were divided into 6 dietary treatments: 1) control, high CP (HCP 20%), 2) medium CP (MCP 18%), 3) low CP (LCP 16%), 4) LCP + 0.20% Gly+Ser, 5) LCP + 0.20% Arg, and 6) LCP + 0.20% Gly+Ser + 0.20% Arg. Although BW gain and feed intake were not affected by treatments, reducing CP to LCP (16% CP) …


Early 1,25-Dihydroxyvitamin D3-Glycosides Supplementation: An Efficient Feeding Strategy Against Bacterial Chondronecrosis With Osteomyelitis Lameness In Broilers Assessed By Using An Aerosol Transmission Model, Andi Asnayanti, Khawla Alharbi, Anh Dang Trieu Do, Layla Al-Mitib, Kathrin Buhler, Jan Dirk Van Der Klis, Javier Gonzalez, Michael T. Kidd, Adnan A. Alrubaye Sep 2024

Early 1,25-Dihydroxyvitamin D3-Glycosides Supplementation: An Efficient Feeding Strategy Against Bacterial Chondronecrosis With Osteomyelitis Lameness In Broilers Assessed By Using An Aerosol Transmission Model, Andi Asnayanti, Khawla Alharbi, Anh Dang Trieu Do, Layla Al-Mitib, Kathrin Buhler, Jan Dirk Van Der Klis, Javier Gonzalez, Michael T. Kidd, Adnan A. Alrubaye

Poultry Science Faculty Publications and Presentations

Bacterial chondronecrosis with osteomyelitis (BCO) lameness is a leg disorder in broilers leading to huge economic losses, food safety concerns, and animal welfare issues in the poultry industry. The active metabolite of vitamin D3, 1,25-dihydroxyvitamin D3, plays pivotal roles in mineral homeostasis, bone health, and immune systems which are essential for protection against BCO. Thus, we hypothesized that supplementation with a Solanum glaucophyllum-derived source of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3-glycosides) would be an effective measure to control lameness. Here, we report the optimum concentration of 1,25(OH)2D3-glycosides …


Testing Feed Is Vital For Feed Budgeting, Department Of Primary Industries And Regional Development, Western Australia Sep 2024

Testing Feed Is Vital For Feed Budgeting, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

A little knowledge of the nutritional value of your supplementary feeds can save you a lot of money and hassle.

In dry seasons, it is likely that farmers will need to start supplementary feeding earlier than usual. The higher requirement for supplementary feeds over dry seasons means accurate feed budgeting becomes increasingly important to minimise feed wastage, for weaner survival, and to maintain stock at the right condition score (CS) for joining.


Native Budworm Spraying Threshold, Department Of Primary Industries And Regional Development, Western Australia Sep 2024

Native Budworm Spraying Threshold, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

The number of native budworm caterpillars in a crop is the major factor determining potential economic damage from the pest. Table 1 was generated with results from department trials to provide an estimate of potential loss from native budworm damage.

Crop loss (kilograms per hectare (kg/ha)) for each caterpillar netted in 10 sweeps (or found per square metre (m2 )) is shown in Table 1. One caterpillar netted in 10 sweeps is equivalent to about 20,000 caterpillars/ha for most pulse crops, assuming an even distribution over the crop


Seal Counting On Our Plages (S.C.O.O.P.), Kaanan Kharwa Sep 2024

Seal Counting On Our Plages (S.C.O.O.P.), Kaanan Kharwa

Master's Theses

The Vertebrate Integrative Physiology (VIP) lab monitors the population of northern elephant seals at the largest mainland breeding colony, located at Piedras Blancas (San Simeon, CA). As the population expands, more human-seal interactions and conflicts over land use occur. The VIP lab's work informs California State Parks and helps with the management of the rookery. Currently, members of the VIP lab fly a drone over the beaches, capture multiple images, and manually count the seals, which takes around 14 to 21 hours of analysis per survey. Machine learning methods such as Convolutional Neural Networks (CNN) and Region-based Convolutional Neural Networks …


Hexarelin Modulates Stress Effects On Ghrelin System Activity In Growing Lambs, Krystina Pierzchała-Koziec, C. G. Scanes, Alina Gajewska Sep 2024

Hexarelin Modulates Stress Effects On Ghrelin System Activity In Growing Lambs, Krystina Pierzchała-Koziec, C. G. Scanes, Alina Gajewska

Political Science Faculty Publications and Presentations

Hexarelin modulates stress effects on ghrelin system activity in growing lambs. Folia Biologica (Kraków) 72 : 87-96. Hexarelin is a synthetic ligand for the growth hormone secretagogue receptor 1a (GHSR-1a), also known as the ghrelin receptor. Ghrelin and hexarelin have been observed to influence the HPA axis by increasing the circulating concentrations of both adrenocorticotropin and adrenal glucocorticoid in rats and humans. The aim of this study was to assess the effects of hexarelin on the ghrelin system and the growth hormone (GH)–insulin-like growth factor 1 (IGF-1) axis in lambs stressed by an emotional factor – isolation. The isolation stress …


Monitoring Marine Animal Interactions With Open Ocean Aquaculture And The Potential For Measuring Impacts, Ryan Richard Yont Aug 2024

Monitoring Marine Animal Interactions With Open Ocean Aquaculture And The Potential For Measuring Impacts, Ryan Richard Yont

Theses

Aquaculture, the farming of water-based organisms, is one of the fastest growing industries in the food production sector globally. Aquaculture farms, regardless of type, are categorized as either coastal or offshore marine aquaculture and are generalized into three categories: fed (e.g., finfish), unfed (e.g., filter-feeding bivalves), and autotrophic (e.g. kelp). Viewed as an agricultural industry, marine aquaculture is tightly regulated, particularly in the U.S. Despite this, an area of concern for the public and management alike is the potential for marine life interactions with aquaculture gear. Even the best-run farms represent a change in the physical and biological characteristics of …


Methodologies For Dry Fixation And Taxidermy Of Education-Oriented Scorpion Specimens, Victoria Tang Aug 2024

Methodologies For Dry Fixation And Taxidermy Of Education-Oriented Scorpion Specimens, Victoria Tang

Euscorpius

Scorpions hold a renowned status as iconic creatures across numerous cultures in the world. Traditionally preserved as desiccated specimens for educational purposes, they have been exhibited in museums and pedagogic collections, serving as concrete epistemological conduits for public education on biodiversity. However, these specimens frequently lack meticulous organization, potentially misrepresenting the animals’ ethological characteristics. This brief article aims to offer paradigmatic guidance for taxidermy of scorpions, ensuring a more accurate depiction of their in vivo habitus, achievable through the use of readily obtainable tools and undemanding techniques.


Oregon State Rank Assessment For Salamander Slug (Gliabates Oregonius), Eleanor P. Gaines Aug 2024

Oregon State Rank Assessment For Salamander Slug (Gliabates Oregonius), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for salamander slug (Gliabates oregonius) using NatureServe methodology, 2024.


Terrestrial Salinization Impacts On Growth And Behavior Of A Common Detritivore Armadillidium Vulgare (Isopoda), Danielle Harmoney Orcutt Aug 2024

Terrestrial Salinization Impacts On Growth And Behavior Of A Common Detritivore Armadillidium Vulgare (Isopoda), Danielle Harmoney Orcutt

Master's Theses

Climate change and human activities are changing the nutritional landscape by increasing the availability of some essential elements and decreasing others. Sodium (Na) is an essential element in heterotrophs, which is required for water regulation, nerve function, and muscle function. Heterotrophs can acquire Na through diet and environment, often experiencing limited availability of Na due to plants concentrating Na 10-100 times less than heterotrophs need. The Sodium Subsidy-Stress (SSS) hypothesis posits that organismal performance follows a hump-shaped curve of a physiological response, where Na acts as a subsidy, increasing organismal performance such as growth until an optimal threshold is reached, …


Effect Of Heat Stress And Feed Type On Trimethylamine Concentration And Fishy Odor Of Duck Eggs, Pachara Pearodwong, Winai Kaewlamun, Sarawanee Khunmanee, Thuwanon Boonkerd, Thananon Junsuri, Kajohn Nitiwararak, Pantira Hempattarasuwan, Pattrarat Chanchaithong Aug 2024

Effect Of Heat Stress And Feed Type On Trimethylamine Concentration And Fishy Odor Of Duck Eggs, Pachara Pearodwong, Winai Kaewlamun, Sarawanee Khunmanee, Thuwanon Boonkerd, Thananon Junsuri, Kajohn Nitiwararak, Pantira Hempattarasuwan, Pattrarat Chanchaithong

The Thai Journal of Veterinary Medicine

We investigated the effect of heat stress and feed type on the trimethylamine (TMA) concentration and the fishy odor of duck eggs (Study 1) and the relationship between the TMA level in serum and egg content (Study 2). In Study 1, pooled egg samples (n = 75) were divided by feed type (CP12, n = 24; CP18, n = 27; CP20, n = 24) and heat stress (no, n = 49; moderate, n = 15; severe, n = 11). The collected samples were obtained for TMA analysis and sensory testing. In Study 2, a total of 15 egg and 15 …


Technical Note: Assessing Gps Sensor Accuracy Using Real-Time Kinematic Device For Livestock Tracking, Biquan Zhao, Rebecca Mcdermott, Galen E. Erickson, Yijie Xiong Aug 2024

Technical Note: Assessing Gps Sensor Accuracy Using Real-Time Kinematic Device For Livestock Tracking, Biquan Zhao, Rebecca Mcdermott, Galen E. Erickson, Yijie Xiong

Department of Animal Science: Faculty Publications

The technical aspects of global positioning system (GPS) sensors have been improved substantially over the years, making them valuable and popular tools for livestock tracking. Using GPS sensors allows producers and researchers to locate grazing livestock, monitor their behavior and distribution, and gather referencing information about the health status of the animals and pastures. However, interpreting the behavior of grazing livestock, such as cattle, from GPS data can be difficult due to positioning inaccuracies. Without knowing the positioning accuracy range of GPS sensors, achieving high-level confidence in determining grazing densities and identifying abnormal livestock movement patterns is challenging. …


Three New Species Of Genus Scorpiops Peters, 1861 From Tibet, China (Scorpiones: Scorpiopidae), With Implications For The Diagnostic Values Of Qualitative Characters, Victoria Tang, Kaichen Ouyang, Zhenbang Liu, František Šťáhlavský Aug 2024

Three New Species Of Genus Scorpiops Peters, 1861 From Tibet, China (Scorpiones: Scorpiopidae), With Implications For The Diagnostic Values Of Qualitative Characters, Victoria Tang, Kaichen Ouyang, Zhenbang Liu, František Šťáhlavský

Euscorpius

Three new species of genus Scorpiops Peters, 1861 are described from Tibet Autonomous Region, China. S. deshpandei sp. n. is described based on an adult male and an immature pair collected from Mêdog County, Nyingchi, with additional molecular evidence substantiating its validity against S. longimanus Pocock, 1893. S. kovariki sp. n. is featured by a single adult female from Zayü. This species is only weakly supported by its genetic distance from S. jendeki Kovařík, 1994, but empirical understanding of the morphological variation in Scorpiops species motivated us to consider them as distinct. S. matthewi sp. n. is based on …


Improving Bovine Intracytoplasmic Sperm Injection, Lauren Gatenby Aug 2024

Improving Bovine Intracytoplasmic Sperm Injection, Lauren Gatenby

LSU Doctoral Dissertations

Despite large efforts, there has been minimal improvements in bovine intracytoplasmic sperm injection (ICSI) in the last few decades. In this study, we investigate methods to improve embryo development rates with ICSI in cattle through addressing sperm and oocyte factors and modifying injection methods. Inducing the acrosome reaction in bull sperm prior to injection with progesterone increased cleavage (46.2% vs 16.2%) and blastocyst (21% vs 3.8%) rates compared to controls, respectively. Supplementing glutathione during in vitro maturation of the oocyte did reduce free reactive oxygen species (ROS) in the oocyte, but did not improve embryo development rates with ICSI or …


Preventing And Managing Equine Gastric Ulcer Syndrome, Kylie Bell, Karl Hoopes Aug 2024

Preventing And Managing Equine Gastric Ulcer Syndrome, Kylie Bell, Karl Hoopes

All Current Publications

Current industry trends regarding equine management practices largely contribute to the development of equine gastric ulcer syndrome (EGUS). Research on effective medical management exists; however, we lack studies on the efficacy of equine-welfare-minded solutions. This fact sheet reviews current literature, including diagnosing, treating, and preventing EGUS. It also provides a general overview of the natural equine, promoting a greater understanding of the connection between strategies to prevent and manage EGUS.


Euscorpius Gulhanimae Sp. N. From The Konya Province, Turkey (Scorpiones: Euscorpiidae), Ersen A. Yağmur Aug 2024

Euscorpius Gulhanimae Sp. N. From The Konya Province, Turkey (Scorpiones: Euscorpiidae), Ersen A. Yağmur

Euscorpius

A new species Euscorpius gulhanimae sp. n. is described from southern Turkey (Beyşehir District of Konya Province). It differs by trichobothrial numbers in Pv and et series; these numbers are the highest in all species of Euscorpius in Turkey. E. gulhanimae sp. n. has light greyish yellow mesosoma, dark reddish-brown chela and dark brown metasomal segments III-V; the new species is distinguished by this two-colored feature from all Euscorpius species. With this description, the known number of species in the family Euscorpiidae in Turkey is increased to 21.


Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xxxiii. Three New Species Of Gint From Ethiopia And Somaliland (Buthidae), František Kovařík, Hassan Sh A. Elmi, František Šťáhlavský Aug 2024

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xxxiii. Three New Species Of Gint From Ethiopia And Somaliland (Buthidae), František Kovařík, Hassan Sh A. Elmi, František Šťáhlavský

Euscorpius

Three new species, Gint sahil sp. n. from Somaliland and Gint abshiri sp. n. and G. derbiae sp. n. from Ethiopia are described and compared with other species of the genus. Additional information is provided on the taxonomy and distribution of the genus Gint, fully complemented with color photos of specimens of the new species, as well as of their habitats. Furthermore, alongside the analyses of external morphology and hemispermatophores, we have provided description of the karyotype of G. abshiri sp. n. This species exhibits karyotype with 2n=27. Included is distribution map and a key for the genus Gint …


Dietary Milk Phospholipids Increase Body Fat And Modulate Gut Permeability, Systemic Inflammation, And Lipid Metabolism In Mice, Albert Lihong Zhou, Robert E. Ward Aug 2024

Dietary Milk Phospholipids Increase Body Fat And Modulate Gut Permeability, Systemic Inflammation, And Lipid Metabolism In Mice, Albert Lihong Zhou, Robert E. Ward

Nutrition, Dietetics, and Food Sciences Faculty Publications

The study aimed at how dietary milk polar lipids affect gut permeability, systemic inflammation, and lipid metabolism during diet-induced obesity (DIO). C57BL/6J mice (n = 6x3) were fed diets with 34% fat as energy for 15 weeks: (1) modified AIN-93G diet (CO); (2) CO with milk gangliosides (GG); (3) CO with milk phospholipids (MPL). Gut permeability was assessed by FITC-dextran and sugar absorption tests. Intestinal tight junction proteins were evaluated by Western blot. Plasma cytokines were measured by immunoassay. Body composition was assessed by magnetic resonance imaging. Tissue lipid profiles were obtained by thin layer chromatography. Hepatic expression of genes …


Revision Of The Genus Scorpio In Jordan, With A Description Of A New Genus And Three New Species (Scorpiones: Scorpionidae), Bassam Abu Afifeh, Ersen A. Yağmur, Mohammad Al–Saraireh, Zuhair Amr Aug 2024

Revision Of The Genus Scorpio In Jordan, With A Description Of A New Genus And Three New Species (Scorpiones: Scorpionidae), Bassam Abu Afifeh, Ersen A. Yağmur, Mohammad Al–Saraireh, Zuhair Amr

Euscorpius

Several new Scorpio populations are studied from various habitats in Jordan, including specimens previously identified as Scorpio kruglovi Birula, 1910 and S. fuscus (Ehrenberg, 1829). A new scorpionid genus, Jordanius gen. n. is described, mainly differentiated from the genus Scorpio Linné, 1758 by the elongation and heavy patterns of granulation of chela manus, and granulated chela fingers. Scorpio granulomanus Al-Saraireh et al., 2023 is transferred to the new genus, and Jordanius maysaraensis gen. et sp. n. is described. Also, Scorpio jordanensis sp. n. and S. wahbehi sp. n. are described. Detailed …


Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış Aug 2024

Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış

The Thai Journal of Veterinary Medicine

Mammary tumors are the most common neoplasm in dogs, with a high mortality rate. The development of canine mammary tumors (CMT) is multifactorial, but different incidence rates between breeds suggest the effects of genetic risk factors. Many CMT-associated candidate genes were reported, mainly involved in cell cycle control. This study aims to investigate p27 tumor suppressor gene polymorphisms in dogs with mammary tumors and analyze the association between variations and CMTs. For this purpose, case and control groups were formed from 22 dogs diagnosed with malignant mammary tumors and 10 dogs with healthy mammary glands. The whole canine p27 gene …


The Molecular Detection Of Hemotropic Mycoplasma Species In Dogs, Hafize Tuğba Yüksel Dolgun, Şükrü Kirkan Aug 2024

The Molecular Detection Of Hemotropic Mycoplasma Species In Dogs, Hafize Tuğba Yüksel Dolgun, Şükrü Kirkan

The Thai Journal of Veterinary Medicine

Hemotropic mycoplasmas are uncultured bacteria that cause different degrees of hemolytic anemia in infected hosts. Molecular approaches are used to diagnose hemotropic mycoplasmas since they are difficult to identify using conventional methods. The aim of this study was to use molecular approaches to determine hemotropic mycoplasmas in dogs. Blood samples were collected in tubes (with EDTA) from 100 dogs. After DNA extraction, 100 DNA samples were investigated by PCR. Hemotropic mycoplasma was detected in 12 (12%) samples and positive samples were subjected to Sanger sequencing. Mycoplasma haemocanis was detected in 6 (50.0%) of the samples, Candidatus Mycoplasma hematoparvum in 5 …


Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong Aug 2024

Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the effects of hydrogel containing nanosilver particles on a second-degree burn wound in a porcine model. The second degree burn wound was performed using 1 cm in diameter, on 100 °C heated cylindrical brass bar applied to the dorsum of pig skin. Burn wounds were randomly divided into three groups of wound dressings (WDs) including commercial polyurethane hydrogel (Cutinova Hydro®, Gr. A), in-house hydrogel (Gr. B) and in-house nanosilver hydrogel (Gr. C). The wound contraction rate and hyperemic zone were measured and investigated at 3, 7, 14 and 21 days after dressing. …


Molecular Characterization And Antibiogram Of Riemerella Anatipestifer In Ducklings In Upper Egypt, Mohamed Sabry Ahmed, Amal Abd El-Ghani Batahi, Ahmed Ibrahim Ahmed, Nabila Osman Aug 2024

Molecular Characterization And Antibiogram Of Riemerella Anatipestifer In Ducklings In Upper Egypt, Mohamed Sabry Ahmed, Amal Abd El-Ghani Batahi, Ahmed Ibrahim Ahmed, Nabila Osman

The Thai Journal of Veterinary Medicine

Riemerella anatipestifer infection represents a great threat to the duck industry worldwide and it is considered the major cause of death in the backyard rearing system of ducks in Egypt. The disease is characterized by diarrhea, respiratory signs and loss of weight. The present study was conducted during the period between July 2019 and January 2021 to examine the prevalence rate, the antibiogram patterns of the different antibiotics and the pathogenicity of R. anatipestifer in Qena province. One-hundred samples were collected from diseased and freshly dead backyard ducklings. A tentative characterization of R. anatipestifer was done depending on the phenotypic …


The Benefits Of Dexmedetomidine For Pain Mitigation After Cautery Disbudding In Calves, Tsung-Ching Liu, Chen-Fei Chiang, Thomas Chia-Tang Ho, Jacky Peng-Wen Chan Aug 2024

The Benefits Of Dexmedetomidine For Pain Mitigation After Cautery Disbudding In Calves, Tsung-Ching Liu, Chen-Fei Chiang, Thomas Chia-Tang Ho, Jacky Peng-Wen Chan

The Thai Journal of Veterinary Medicine

The purpose of this study was to determine the effect of dexmedetomidine (DEX) alone on pain mitigation following cautery disbudding in calves. A total of 21 calves were randomly divided into three groups: sham-disbudded control with placebo (S, n = 8); hot-iron disbudded after placebo (D, n = 6); hot-iron disbudded after 0.01 mg/kg of DEX (DeD, n = 7). The pain-related responses measured included serum cortisol levels, struggling scores, pain-related behaviors and other physiological values. The results indicated that the struggling scores, the cortisol concentrations from +10 to +60 min after disbudding and the area under curve of cortisol …


Productivity Of Young Broilers Fed Phyllanthus Niruri Linn And Melaleuca Cajuput Leaf Meal As A Phytogenic Feed Additive, Listya Purnamasari Aug 2024

Productivity Of Young Broilers Fed Phyllanthus Niruri Linn And Melaleuca Cajuput Leaf Meal As A Phytogenic Feed Additive, Listya Purnamasari

The Thai Journal of Veterinary Medicine

Feed additives from medicinal plants can be used as alternative antibiotics to optimize productivity, growth, and health of livestock. Medicinal plants Phyllanthus niruri Linn (Meniran) and Melaleuca cajuput (Kajuput) as alternative growth promoters contain antioxidants and antimicrobials to improve the quality of animal products. The aim of the study was to evaluate the effect of medicinal plant leaf meals as phytogenic feed additives on broilers’ productivity and internal organ parameters. For 21 days fed, 80 1-day-old Loughmann broilers (MB202) unsex were distributed in 4 treatments with 4 pens per treatment and 5 birds per pen. The dietary treatments were: T1 …


Image Processing And Machine Learning Approach For Yolk Color Evaluation, Supreeya Thepparak, Chusak Pulmar, Chanwit Kaewtapee Aug 2024

Image Processing And Machine Learning Approach For Yolk Color Evaluation, Supreeya Thepparak, Chusak Pulmar, Chanwit Kaewtapee

The Thai Journal of Veterinary Medicine

The objective of this study was to develop a predictive model for yolk color measurement using machine learning. A total of eight hundred eggs were randomly collected from thirty laying hens (Lohmann brown, 28–35 weeks). The experimental diets were formulated based on broken rice or corn, and further supplemented with canthaxanthin from 10 to 150 mg/kg. The Digital YolkFan™ was used to classify yolk color scales from 0 to 15, whereas the Hunter Lab was used to measure color values. Furthermore, yolk images were obtained from a digital camera and then extracted into red color, green color and blue (RGB) …


Single-Tank Sequencing Batch Bioreactor With A Cellulose Substrate For Simultaneous Nitrification And Denitrification Of An Aquarium, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Sanit Piyapattanakorn, Surachet Burut-Archanai Aug 2024

Single-Tank Sequencing Batch Bioreactor With A Cellulose Substrate For Simultaneous Nitrification And Denitrification Of An Aquarium, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Sanit Piyapattanakorn, Surachet Burut-Archanai

The Thai Journal of Veterinary Medicine

Processing and detoxifying nitrogenous waste in an aquarium is difficult with one tank because nitrifying and denitrifying bacteria need contradictory conditions to grow and survive. Herein, a single-tank sequencing batch bioreactor was used to simultaneously perform aerobic nitrification and denitrification for aquarium filtration. To evaluate its performance at the laboratory-scale, the 8 L bioreactor was connected to a 20 L aquarium with a carbon water filter and containing Oranda goldfish. The bioreactor comprised a bottom layer filled with 2.5 L of polyester sponge, covered with 1 L of carbon pellets and then 2.5 L of cellulose beads or polyester sponge. …


Phylotranscriptomic Relationships Of The Oophila Clade Of Green Algae Associated To Amphibian Egg Masses, Ryan Kerney, Miguel Vences, Maria Sachs, Iker Irisarri, Fabian Bartels, Pontus Eriksson, Sven Künzel, Atsushi Kurabayashi, Ane Laugen, Zachary Vegso, Cory Bishop, Hartmut Arndt Aug 2024

Phylotranscriptomic Relationships Of The Oophila Clade Of Green Algae Associated To Amphibian Egg Masses, Ryan Kerney, Miguel Vences, Maria Sachs, Iker Irisarri, Fabian Bartels, Pontus Eriksson, Sven Künzel, Atsushi Kurabayashi, Ane Laugen, Zachary Vegso, Cory Bishop, Hartmut Arndt

Biology Faculty Publications

Green algae usually assigned to the genus Oophila are known to colonize egg capsules of amphibian egg masses across the Nearctic and Palearctic regions. We study the phylogenetic relationships of these algae using a phylotranscriptomic data set of 76 protein-coding single-copy nuclear genes. Our data set includes novel RNAseq data for six amphibian-associated and five free-living green algae, and draft genomes of two of the latter. Within the Oophila clade (nested within Moewusinia), we find samples from two European frogs (Rana dalmatina and R. temporaria) closely related to those of the North American frog R. aurora (Oophila …


A Comparison Of Diploid And Triploid Eastern Oysters For Aquaculture Production Under Extreme Temperatures And Salinities, Joshua H. Kim Aug 2024

A Comparison Of Diploid And Triploid Eastern Oysters For Aquaculture Production Under Extreme Temperatures And Salinities, Joshua H. Kim

LSU Master's Theses

Off-bottom aquaculture of Eastern oysters (Crassostrea virginica) is a nascent industry that is increasingly supported by the use of triploid oysters, which grow faster than diploids. Despite their growth advantage, elevated triploid mortality compared to diploids under high temperature, low salinity, or a combination of these conditions challenge consistent triploid production. Identifying the environmental thresholds at which differential triploid mortality occurs and predicting its economic impact are important to informing decision-making in oyster aquaculture. The goal of this thesis was to compare the biological and economic performance of diploid and triploid oysters under high temperature and low salinity conditions. …


An Indirect Elisa Using A Recombinant Envelope Protein For Detecting Antibody Against Duck Tembusu Virus Infection, Thaweesak Songserm Aug 2024

An Indirect Elisa Using A Recombinant Envelope Protein For Detecting Antibody Against Duck Tembusu Virus Infection, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Duck Tembusu virus (DTMUV) belonging to Flavivirus group, causes severe central nervous system disorders and decreased egg production as found in Thailand. To detect antibody against the DTMUV infection, we developed an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant envelop (E) protein for coating antigen. The E protein coding gene of KPS54A61DTMUV was cloned into pPICzαA vector and expressed by using 2% (v/v) of absolute methanol as inducer in Pichia pastoris expression. The recombinant E protein was detected in the culture medium at a concentration of 4.55 mg/ml and analyzed a protein expected size by SDS-PAGE at 65 …