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Current Trends And Experiences From A Contract Research Organization Perspective, Dan Moore Sep 2024

Current Trends And Experiences From A Contract Research Organization Perspective, Dan Moore

Proceedings of the Arkansas Nutrition Conference

The primary goal of this presentation is to provide an update on the trends of research observed at a contract research organization (CRO) in recent years as it relates to species, study design type and products tested, as well as, challenge models used and products that have shown benefits in various study designs. The information in the presentation is based off of studies conducted from 2014-2024 at one CRO, Colorado Quality Research (CQR), and represents the experiences at CQR. Many of the parameters discussed will be from 2014-2019 and 2020-2024 to observe trends from over 5 years ago and more …


Importance Of A Rooster Feed In Broiler Breeder Production, Alex Corzo, Marcelo Silva Sep 2024

Importance Of A Rooster Feed In Broiler Breeder Production, Alex Corzo, Marcelo Silva

Proceedings of the Arkansas Nutrition Conference

Of all feed phases in poultry production, rooster feeding is arguably the least studied. During the rear phase, there is general agreement that male and female broiler breeders can be provided a common feed program, only differing on feed amounts provided, that results in growth targets reached for each sex. It is during the production phase that there is some disagreement on the best way to support the nutritional needs of the adult rooster. A considerable segment of the world does employ a rooster feed as a way to better support the nutritional needs of working males. Other poultry operations …


Vitamin Update And Sustainability, Edgar Orlando Oviedo-Rondón Sep 2024

Vitamin Update And Sustainability, Edgar Orlando Oviedo-Rondón

Proceedings of the Arkansas Nutrition Conference

This presentation addressed the status of research on vitamin nutrition in poultry species and current recommended levels. The economic, environmental, and socio-political aspects of sustainability are addressed. Most of the information presented is described in detail in the book Optimum Vitamin Nutrition for More Sustainable Poultry Farming (Oviedo-Rondon et al., 2023). However, this presentation also used more recent publications and news of the latest events related to scientific recommendations and the worldwide vitamin market. The objective is to provide a brief overview of scientific findings to aid nutritionists in the decision-making process when selecting optimum levels for poultry nutrition. …


Every Day Feeding Of Broiler Breeder Pullets, David Brandt Sep 2024

Every Day Feeding Of Broiler Breeder Pullets, David Brandt

Proceedings of the Arkansas Nutrition Conference

Broiler breeders are characterized by their fast growth and sensitivity to light stimuli. Unlike commercial layers, they have a lower egg production rate and are kept on the floor during rearing and laying periods. Fertile eggs are the primary objective, necessitating careful management of their egg-laying properties, while controlling their body weights. Unrestricted feeding leads to early laying with poor peak and persistency in egg production. Therefore, nutrient intake must be restricted to achieve target body weights at light stimulation (22 weeks). This involves both quantitative and qualitative feed restrictions. Quantitative feed restriction can lead to issues such as poor …


Integration Of Commercial Broiler Data Into A Broiler Growth Model, David Brandt Sep 2024

Integration Of Commercial Broiler Data Into A Broiler Growth Model, David Brandt

Proceedings of the Arkansas Nutrition Conference

This study explores the alignment of theoretical broiler growth models with practical farm conditions, emphasizing the value of integrating commercial data into simulation models. The EFG Broiler Growth Model, developed by Emmens, Fisher and Gous, is highlighted for its mechanistic approach, predicting growth based on feed intake. While empirical models are useful, they are limited by changes in genetics. Both mechanistic and empirical models struggle to predict how broilers will respond to feed under the challenging conditions typically experienced in commercial operations. The study also discusses the development of empirical models to determine broiler responses to balanced protein and energy, …


Estimation Of Bovine Tuberculosis Prevalence, Associated Risk Factors, And Public Health Significance In District Dera Ismail Khan, Arsalan Khan, Akhtar Ali, Umm E. Aimen, Wasim Ullah, Imtiaz Khan, Qamar Ullah, Baqir Hussain, Muneeb Ur Rahman Sep 2024

Estimation Of Bovine Tuberculosis Prevalence, Associated Risk Factors, And Public Health Significance In District Dera Ismail Khan, Arsalan Khan, Akhtar Ali, Umm E. Aimen, Wasim Ullah, Imtiaz Khan, Qamar Ullah, Baqir Hussain, Muneeb Ur Rahman

Journal of Bioresource Management

Bovine tuberculosis (bTB) is zoonotic disease of global concern, affecting both animal and human populations. To estimate the prevalence of bovine TB in District Dera Ismail Khan and identify associated risk factors. A total of 138 cattle from different herds were included in the survey, and diagnostic tests, including the tuberculin skin test and Ziehl Neelsen stain, were employed. The results revealed a high prevalence of bovine TB, with an estimated prevalence of 10.87 % in the study area. Prevalence analysis based on tehsils revealed varying rates: 6.66 % in Dera Ismail Khan, 11.11 % in Kulachi, and 14.81 % …


Impact Of Bypass Fat Supplementation On Milk Production And Weight Gain In Nili Ravi Buffaloes Under Southern Khyber Pakhtunkhwa's Geo-Climatic Conditions, Shahzeb Khan, Arsalan Khan, Asad Ullah, Abid Ullah, Amjad Ali, Safi Ullah, Imran Khan, Muhammad Yasir, Israr Ud Din, Atta Ur Rehman, Muhammad Rizwan, Jahangir Ali Sep 2024

Impact Of Bypass Fat Supplementation On Milk Production And Weight Gain In Nili Ravi Buffaloes Under Southern Khyber Pakhtunkhwa's Geo-Climatic Conditions, Shahzeb Khan, Arsalan Khan, Asad Ullah, Abid Ullah, Amjad Ali, Safi Ullah, Imran Khan, Muhammad Yasir, Israr Ud Din, Atta Ur Rehman, Muhammad Rizwan, Jahangir Ali

Journal of Bioresource Management

This study was conducted to examine the effect of bypass fat supplementation on milk production, composition, and body weight gain in Nili Ravi Buffaloes living in the unique geo-climatic conditions of Southern Khyber Pakhtunkhwa. Twenty lactating Nili Ravi buffaloes were randomly assigned to control and bypass fat supplementation groups in randomized, controlled experiment. Over the course of three months, data were collected on milk yield, milk composition, and body weight while environmental factors were considered. The bypass fat group gained significantly more weight on average than the control (p0.05). Bypass fat supplementation can substantially increase milk production, milk fat content, …


Ameliorations Of Olea Ferruginea Fruit Pulp Extract In Ccl4 Induced Testicular Pathology In Mice, Syeda Nadia Ahmad, Tasleem Ishaq, Muhammad Ali Kanwal, Iram Inayat, Usma Abdullah, Khawaja Raees Ahmad Sep 2024

Ameliorations Of Olea Ferruginea Fruit Pulp Extract In Ccl4 Induced Testicular Pathology In Mice, Syeda Nadia Ahmad, Tasleem Ishaq, Muhammad Ali Kanwal, Iram Inayat, Usma Abdullah, Khawaja Raees Ahmad

Journal of Bioresource Management

In present study the testicular histo-ameliorations of wild olive (Olea ferruginea) fruit pulp extract (OFPE) against intra-gastric CCl4 exposure in mice was investigated. Thirty male albino mice were allocated into three groups of ten each: 1) Vehicle control (Vc); 2) CCl4 (C); and 3) CCl4+ Olea ferruginea (COf). All groups, except the Vc, were administered CCl4 (0.1mL of a 0.2mLkg−1 solution) in corn oil. Over the subsequent five days, the COf group received 0.1mL of Olea ferruginea plant extract (OFPE) daily, while the Vc and CCl4 groups were provided water in …


A Comprehensive Study On One Health Strategy And Public Health Effects Of Salmonella, Muhammad Aqeel, Amjad Hussain Mirani, Parvez Ahmed Khoso, Jam Kashif Sahito, Riaz Ahmed Leghari, Abdul Latif Bhutto, Muhammad Mohsen Rahimoon, Abdul Azeem Khoso, Rasool Bux, Azmat Aliasn Arsalan Ali Sep 2024

A Comprehensive Study On One Health Strategy And Public Health Effects Of Salmonella, Muhammad Aqeel, Amjad Hussain Mirani, Parvez Ahmed Khoso, Jam Kashif Sahito, Riaz Ahmed Leghari, Abdul Latif Bhutto, Muhammad Mohsen Rahimoon, Abdul Azeem Khoso, Rasool Bux, Azmat Aliasn Arsalan Ali

Journal of Bioresource Management

Salmonella poses a significant public health challenge due to its antibiotic resistance, zoonotic transmission, and diverse clinical manifestations. Over 60 % of human diseases are zoonotic, influenced by ecological dynamics and human activities such as land use changes, population growth, and international travel. Foodborne diseases, particularly those caused by Salmonella spp., have a substantial global impact, especially in low-income countries. Salmonella is divided into two species, Salmonella enterica and Salmonella bongori, with more than 2000 serotypes and six subspecies within S. enterica. This bacterium is highly adaptable, surviving extreme conditions such as drying, high salt concentrations, and acidic …


Effect Of Curcuma Longa On Growth And Lipid Profile Of Broiler Chickens Across Seasons, Sehrish -, Roheela Yasmeen Sep 2024

Effect Of Curcuma Longa On Growth And Lipid Profile Of Broiler Chickens Across Seasons, Sehrish -, Roheela Yasmeen

Journal of Bioresource Management

The poultry sector plays a critical role in driving economic growth and meeting the protein needs of the population. Recently, natural additives like turmeric (Curcuma longa) have attracted attention for their antimicrobial properties and their potential to reduce the reliance on antibiotics in poultry feed. This study evaluated the effects of turmeric supplementation at varying concentrations on broiler chickens during winter and summer seasons. Over a 42-day period, 40 broilers were divided into four groups: Control, Experimental Group 1 (1% turmeric), Experimental Group 2 (1.5% turmeric), and Experimental Group 3 (2% turmeric). Vaccination against Newcastle Disease (ND) and …


Law School News: Supporting Rhode Island's Aquaculture 9-23-2024, Andrew Clark, Roger Williams University School Of Law Sep 2024

Law School News: Supporting Rhode Island's Aquaculture 9-23-2024, Andrew Clark, Roger Williams University School Of Law

Life of the Law School (1993- )

No abstract provided.


Semiautomated Generation Of Species-Specific Training Data From Large, Unlabeled Acoustic Datasets For Deep Supervised Birdsong Isolation, Justin Sasek, Brendan Allison, Andrea Contina, David Knobles, Preston Wilson, Timothy Keitt Sep 2024

Semiautomated Generation Of Species-Specific Training Data From Large, Unlabeled Acoustic Datasets For Deep Supervised Birdsong Isolation, Justin Sasek, Brendan Allison, Andrea Contina, David Knobles, Preston Wilson, Timothy Keitt

School of Integrative Biological & Chemical Sciences Faculty Publications

Background

Bioacoustic monitoring is an effective and minimally invasive method to study wildlife ecology. However, even the state-of-the-art techniques for analyzing birdsongs decrease in accuracy in the presence of extraneous signals such as anthropogenic noise and vocalizations of non-target species. Deep supervised source separation (DSSS) algorithms have been shown to effectively separate mixtures of animal vocalizations. However, in practice, recording sites also have site-specific variations and unique background audio that need to be removed, warranting the need for site-specific data.

Methods

Here, we test the potential of training DSSS models on site-specific bird vocalizations and background audio. We used a …


Effect Of Cytoplasmic Polyadenylation Element Protein Overexpression On The Development Of Bovine Cloned Embryos, Ying Liu Sep 2024

Effect Of Cytoplasmic Polyadenylation Element Protein Overexpression On The Development Of Bovine Cloned Embryos, Ying Liu

Funded Research Records

No abstract provided.


Rethinking Pregnancy Recognition In Ruminants: Pregnancy Recognition In Ewes Lacking Interferon-Tau Signaling, Chris Davies Sep 2024

Rethinking Pregnancy Recognition In Ruminants: Pregnancy Recognition In Ewes Lacking Interferon-Tau Signaling, Chris Davies

Funded Research Records

No abstract provided.


The Impact Of Estradiol And/Or Trenbolone Acetate On The Metabolome And Skeletal Muscle Metabolism Of Beef Cattle, Kara J. Thornton-Kurth Sep 2024

The Impact Of Estradiol And/Or Trenbolone Acetate On The Metabolome And Skeletal Muscle Metabolism Of Beef Cattle, Kara J. Thornton-Kurth

Funded Research Records

No abstract provided.


Animal Breeding-Rcn: Farm Animal Genomics Collective, Noelle E. Cockett Sep 2024

Animal Breeding-Rcn: Farm Animal Genomics Collective, Noelle E. Cockett

Funded Research Records

No abstract provided.


Novel Hamster Models Of Chikungunya Viral Arthritis, Tian Wang, Zhongde Wang Sep 2024

Novel Hamster Models Of Chikungunya Viral Arthritis, Tian Wang, Zhongde Wang

Funded Research Records

No abstract provided.


Oregon State Rank Assessment For Goose Lake Lamprey (Entosphenus Tridentatus Ssp. 1), Eleanor P. Gaines Sep 2024

Oregon State Rank Assessment For Goose Lake Lamprey (Entosphenus Tridentatus Ssp. 1), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Goose Lake lamprey (Entosphenus tridentatus ssp. 1) using NatureServe methodology, 2024.


Oregon State Rank Assessment For Columbia Mottled Sculpin (Cottus Hubbsi), Eleanor P. Gaines Sep 2024

Oregon State Rank Assessment For Columbia Mottled Sculpin (Cottus Hubbsi), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Columbia mottled sculpin (Cottus hubbsi) using NatureServe methodology, 2024.


Predicting Spatiotemporal Patterns Of Productivity And Grazing From Multispectral Data Using Neural Network Analysis Based On System Complexity, Amanda J. Ashworth, A. Avila, H. Smith, T. E. Winzeler, P. Owens, C. Flynn, P. O'Brien, D. Philipp, J. Su Sep 2024

Predicting Spatiotemporal Patterns Of Productivity And Grazing From Multispectral Data Using Neural Network Analysis Based On System Complexity, Amanda J. Ashworth, A. Avila, H. Smith, T. E. Winzeler, P. Owens, C. Flynn, P. O'Brien, D. Philipp, J. Su

Environmental Dynamics Faculty Publications and Presentations

Remote sensing tools, along with Global Navigation Satellite System cattle collars and digital soil maps, may help elucidate spatiotemporal relationships among soils, terrain, forages, and animals. However, standard computational procedures preclude systems-level evaluations across this continuum due to data inoperability and processing limitations. Deep learning, a subset of neural network, may elucidate efficiency of livestock production and linkages within the livestock-grazing environment. Consequently, we applied deep learning to environmental remote sensing data to (1) develop predictive models for yield and forage nutrition based on vegetation indices and (2) at a pixel-level (per 55 m2), identify how grazing is …


Insights Into Past Extreme Environments Through Ancient Biomolecules, Ioana Nicoleta Meleg, Magdalena Năpăruş-Aljancic, Yuichi Naito, Hervé Bocherens, Axel Barlow Sep 2024

Insights Into Past Extreme Environments Through Ancient Biomolecules, Ioana Nicoleta Meleg, Magdalena Năpăruş-Aljancic, Yuichi Naito, Hervé Bocherens, Axel Barlow

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.


Ormeloxifene Suppresses Emt In Hepatocellular Carcinoma By Targeting Β-Catenin Signaling Pathway, Shabnam Malik, Mohammed Sikander, Daniel Zubieta, Anyssa Rodriguez, Murali Yallapu, Meena Jaggi, Subhash C. Chauhan Sep 2024

Ormeloxifene Suppresses Emt In Hepatocellular Carcinoma By Targeting Β-Catenin Signaling Pathway, Shabnam Malik, Mohammed Sikander, Daniel Zubieta, Anyssa Rodriguez, Murali Yallapu, Meena Jaggi, Subhash C. Chauhan

Research Colloquium

Background: Hepatocellular carcinoma (HCC) is one of the deadliest cancers, with a five-year survival rate. While Sorafenib (SRB) is the standard therapy, it is only effective in prolonging survival for some patients, emphasizing the urgent need for alternative treatment options. Resistance to apoptosis is a defining characteristic of cancer cells and a crucial factor in cancer recurrence and relapse. Although, anticancer potential of the FDA-approved compound Ormeloxifene (ORM), which has well-defined pharmacokinetic and pharmacodynamic properties, has been explored in a variety of cancers, its efficacy in HCC has remained unknown. Therefore, the purpose of this study was to investigate the …


Enhanced Bmp Signaling Via Alk2 In Osteoclasts Decreases Bone Density In Mice, Yolanda V. Gutierrez, Hiroyuki Yamaguchi, Yuji Mishina, Yoshihiro Komatsu Sep 2024

Enhanced Bmp Signaling Via Alk2 In Osteoclasts Decreases Bone Density In Mice, Yolanda V. Gutierrez, Hiroyuki Yamaguchi, Yuji Mishina, Yoshihiro Komatsu

Research Colloquium

Bone remodeling is a complex biological process that has been extensively studied. Bone Morphogenetic Proteins (BMPs) are recognized as one of the critical growth factors that coordinate bone remodeling. Previous studies have demonstrated that BMP signaling in osteoclasts has a positive effect on osteoclast function. However, little is known about how each BMP type I receptors control osteoclastogenesis. To investigate this question, we utilized the Cre-LoxP system to specifically activate BMP signaling through ALK2 in mice. We utilized Cathepsin K (Ctsk)-Cre driver to activate BMP signaling in osteoclasts in mice. Compared with aged- and gender-matched controls, gain-of-function of BMP mutant …


Molecular Detection Of Mycoplasma Spp. And Its Associated Risk Factors In Domestic Cats In The City Of Ibagué, Tolima, Karen D. Osorio Muñoz Dr., Iang S. Rondon Barragan Prof. Sep 2024

Molecular Detection Of Mycoplasma Spp. And Its Associated Risk Factors In Domestic Cats In The City Of Ibagué, Tolima, Karen D. Osorio Muñoz Dr., Iang S. Rondon Barragan Prof.

The Thai Journal of Veterinary Medicine

Feline hemoplasmosis is a disease with worldwide distribution, mainly in tropical countries, where its transmission is favored by the presence of vectors such as fleas and ticks. In Colombia, the diagnosis of the disease in small animals has been limited to the identification of the pathogen in blood smears, which have low sensitivity and specificity, demanding methods of higher diagnostic value, such as those based on molecular techniques. The objective of the present study was to investigate the prevalence of Mycoplasma spp. in felines from the city of Ibagué using the PCR and the associated risk factors. Briefly, blood samples …


Risk Factors Of Bovine Clinical Mastitis And Antimicrobial Resistance Of Mastitis-Causing Escherichia Coli In Shandong, China, Liu Peng, Dongfang Hu, Jinjiao Zuo, Caihong Wu, Hui Lu, Zilong Cheng, Mengda Liu Sep 2024

Risk Factors Of Bovine Clinical Mastitis And Antimicrobial Resistance Of Mastitis-Causing Escherichia Coli In Shandong, China, Liu Peng, Dongfang Hu, Jinjiao Zuo, Caihong Wu, Hui Lu, Zilong Cheng, Mengda Liu

The Thai Journal of Veterinary Medicine

The culling of dairy cattle is closely related to the economic benefits of the farm. Many reasons can lead to the culling of cows. Among these, mastitis is the main reason for the serious economic loss of the dairy industry, and also the harm to global public health. However, little is known about cow culling situations, antimicrobial resistance, and virulence factors of mastitis-causing Escherichia coli in Shandong, China. A cross-sectional study was conducted in 16 randomly selected dairy herds to identify the reasons for cow culling, to assess the risk factors, and to isolate the major etiological agent of clinical …


Casein Gene Polymorphism Of Goat In Thailand, Naparat Naowanat, Duangsmorn Suwattana, Boonnarong Suphap Sep 2024

Casein Gene Polymorphism Of Goat In Thailand, Naparat Naowanat, Duangsmorn Suwattana, Boonnarong Suphap

The Thai Journal of Veterinary Medicine

The genetic polymorphism of the casein genes and the evaluation of haplotype variability in Saanen and Thai Native breeds in Thailand were investigated. The 23 Saanen and 42 Thai Native goats were genotyped at CSN1S1, CSN2, CSN1S2, and CSN3 loci using sequencing techniques. Three variants of A, C, and E on the CSN1S1 gene locus were demonstrated. The genotypic frequencies of CSN1S1 AA, AC, and AE are shared by over 80% of the population. The CSN2 A and F alleles were observed. The CSN2 genotypes of AA, AF, and FF were indicated with the majority of AF frequency. The 2 …


Low-Emission Beef Production In The Southern Rangelands Of Western Australia: An Analysis Of Herd Structure And Stocking Rate Experiencing Droughts, Christophe D'Abbadie Sep 2024

Low-Emission Beef Production In The Southern Rangelands Of Western Australia: An Analysis Of Herd Structure And Stocking Rate Experiencing Droughts, Christophe D'Abbadie

Animal production and livestock research articles

Reconciling profitable cattle production with rangeland health and reduced emissions is a key challenge in the southern rangelands of Western Australia (WA). Stocking rate and herd structure selection are crucial decisions to achieve this balance. This study assessed the emission profiles of three contrasting herd structures (weaner production, live export, and slaughter production), and three stocking rates within a herd–carbon accounting modelling framework. The analysis considers the impact of varying drought frequencies on these cattle production systems. Herd models were developed for the semiarid southern WA rangelands. Stocking rates were set at 100%, 80% and 66% of the government recommended …


An Assessment Of Dingo Ancestry In Camp Dogs In Western Australia, Tracey L. Kreplins, Danielle Stephens, N Laing, C Oskam Sep 2024

An Assessment Of Dingo Ancestry In Camp Dogs In Western Australia, Tracey L. Kreplins, Danielle Stephens, N Laing, C Oskam

Biosecurity Research Articles

Hybridisation between Australian dingoes and domestic dogs is a controversial area of interest and research. An ongoing canine sterilisation programme in rural and remote Western Australia provided an opportunity to assess the dingo ancestry of camp dogs and opportunities for hybridisation. Blood samples were collected from 345 individual community dogs at 21 locations. Dogs were screened using 23 microsatellite loci and ancestry percentage assigned using an iterative Bayesian assignment algorithm. A single individual was a dingo, 96% were domestic dogs and 3.5% were hybrids. Camp dog and dingo hybridisation in these areas is of little concern in terms of conserving …


Using Growth Implants In Bos Indicus Cattle Compared To Bos Taurus Cattle, Reganne K. Briggs, Caleb C. Reichhardt, Kara Thornton-Kurth, Matthew Garcia, Laura Motsinger Sep 2024

Using Growth Implants In Bos Indicus Cattle Compared To Bos Taurus Cattle, Reganne K. Briggs, Caleb C. Reichhardt, Kara Thornton-Kurth, Matthew Garcia, Laura Motsinger

All Current Publications

A recent study at Utah State University compared production response to anabolic implant protocols of Santa Gertrudis-sired and Angus-sired steers. Feedlot performance, carcass characteristics, and economic analyses were completed to determine how implants affect Bos indicus-influenced animals. This fact sheet reviews the research findings and suggested benefits, such as increased efficiency and yield of steers.


Serum Proteomic Profile Of Subclinical Babesia Bovis Infection In Pcr-Positive Eld's Deer Using Serum Protein Electrophoresis Coupled With Lc-Ms/Ms, Vichayanee Pumpitakkul, Sittiruk Roytrakul, Narumon Phaonakrop, Gunnaporn Suriyaphol Assoc. Prof. Sep 2024

Serum Proteomic Profile Of Subclinical Babesia Bovis Infection In Pcr-Positive Eld's Deer Using Serum Protein Electrophoresis Coupled With Lc-Ms/Ms, Vichayanee Pumpitakkul, Sittiruk Roytrakul, Narumon Phaonakrop, Gunnaporn Suriyaphol Assoc. Prof.

The Thai Journal of Veterinary Medicine

Subclinical babesiosis observed in captive Eld’s deer, which act as reservoirs for ticks, poses a risk to healthy herds. However, the knowledge of biomarkers for subclinically infected Eld’s deer and host-parasite interactions is limited. This study aimed to investigate the serum proteomic profiles and protein interactions of Babesia bovis and subclinically infected Eld’s deer, PCR-positive for B. bovis, using automated serum protein electrophoresis and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The findings revealed albumin and four globulin fractions: alpha-1, alpha-2, beta, and gamma globulin. Albumin protein (ALB) was consistently detected across all fractions, highlighting its role in maintaining osmotic balance …