Low Salinities Impair Heat Tolerance And Alter Reproductive Traits With No Implications For Survival In A Marine Annelid From Coastal Habitats, Gabrielle Newton, Gloria Massamba-N'Siala

Biological Sciences Faculty Publications

Climate change is expected to intensify salinity and temperature fluctuations in estuarine and coastal habitats. However, the impact of salinity variation on ectotherms’ thermal tolerance remains underexplored, limiting our understanding of the interplay between these stressors. This study investigated salinity-dependent changes in life history traits (survival, growth rate, fecundity, and egg size) over a 33-day exposure to a salinity gradient (12, 17, 22, 27, 32, 35, 40, 45, 50) in the marine annelid Ophryotrocha labronica. Individuals that survived the salinity treatments were assessed for their upper and lower thermal tolerance limits. Female individuals, the target of the study, exhibited …

Hair Growth Rate Estimation In North American Ursids, Jennifer H. Stern, Karyn D. Rode, Craig A. Stricker, Sheriden Ploof, Cindy L. J. Roberts, Celess Edinger, Mary Humbyrd, Nate Wagner, Megan A. Owen, John P. Whiteman, Thea Bechshoft, Brent White, Kristin L. Laidre

Biological Sciences Faculty Publications

The feeding ecology of wildlife populations has important implications for individual health, population productivity and distribution patterns. For ursids (bears), food resources and feeding behaviour primarily affect population dynamics via effects on cub production and survival. Much of what is known about the feeding ecology of bears is based on analyses of tissues collected from capture-based research efforts, harvested animals or non-invasive approaches. However, inference about diet from hair has been limited by a lack of quantitative data on the timing of the moult and hair growth rates. We conducted a study to develop and test two methods of quantifying …

The Prize Biter Of Modernity? Investigating Killer Whale Bite Mechanics, Daniel Hong

West Chester University Graduate Theses, Dissertations, and Final Projects

Although the killer whale (Orcinus orca) is a cosmopolitan apex predator, very little work has been done on its feeding biomechanics. Here I use finite element analysis to investigate the mandibular stiffness of a juvenile male Bigg’s killer whale, and compare it to that of the false killer whale (Pseudorca crassidens) and the saltwater crocodile (Crocodylus porosus). Full mandibles and left and right halves of mandibles are simulated under FEA. I also estimate the total jaw muscle force produced by the Bigg’s killer whale using the dry skull method. The killer whale lacks an …

Investigating The Relationships Between Biotic And Abiotic Factors Affecting Migratory Behavior In Westslope Cutthroat Trout Within The Pend Oreille System, Garrett M. Duncan

EWU Masters Thesis Collection

Salmonids express several different life histories from migrating to oceans and back or staying in their natal streams as a resident. Migratory individuals are a vital part of the overall success of populations due to the potential for increased genetic diversity and increased fecundity they offer over resident individuals. This research was completed in three basins including Priest River, Pack River, and Lightning Creek which are all connected to the Pend Oreille Basin and are important producers of Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi). Thirty-nine sites were sampled between the three basins. Results were compared between these tributaries that have …

Microbial Community Stabilization By Restored Oyster Reefs: Implications For Blue Carbon Storage, Natalie Boydstun

College of Graduate Studies: Theses & Dissertations

Coastal ecosystems provide critical carbon sequestration services, yet the microbial mechanisms underlying carbon storage remain poorly understood, particularly in non-vegetated habitats. This study investigated whether restored oyster reefs (Crassostrea virginica) enhance sediment carbon storage potential by stabilizing microbial communities and promoting efficient organic matter processing. I hypothesized that reef-mediated sediment stabilization and organic deposition would create conditions favoring microbial degradation of complex carbon compounds into stable soil organic matter, ultimately supporting anaerobic carbon storage. To test this, I compared sediment biogeochemistry, enzyme activity, and microbial community structure between three restored oyster reef sites and two unstructured control sites …

Habitat Loss And Its Behavioral Effects On A Female Gray Hawk In The Lower Rio Grande Valley Of Texas, Usa, Michael T. Stewart, Ashley M. Tanner, Brian A. Millsap, William S. Clark

Human–Wildlife Interactions

Habitat loss is a major threat to wildlife worldwide. While habitat loss is commonly associated with the fragmentation, degradation, or destruction of large tracts of suitable space, habitat loss in urban environments can be much more incremental and nuanced. We tracked an adult female gray hawk (Buteo plagiatus) in the Lower Rio Grande Valley of Texas, USA, before and after 70% of the trees (7.6 ha) were cleared from the core of the nesting territory. While the hawk’s overall activity budget between within-patch and exploratory movements was unchanged, the hawk undertook substantially longer exploratory movements after the trees …

Leukocyte Infiltration Profiles At The Site Of Intradermal Injection Of Silikon-1000 In Scleroderma-Prone Ucd200/206 And Healthy White Leghorn Chickens, Katee L. Johnson, Jossie M. Santamaria, Chrysta N. Beck, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Intradermal injections of silicone-based fillers for the face and body may cause chronic inflammation and fibrotic reactions in some individuals. Chickens from the UCD 200/206 White Leghorn (WL) line spontaneously develop a fibrotic autoimmune disease known as scleroderma/systemic sclerosis (SSc). Using the SSc-prone UCD and healthy WL chickens, a longitudinal study was conducted to examine the cellular inflammatory responses to intradermal (i.d.) injections of sterile SILIKON-1000. Specifically, the pulp (a skin derivative) of growing feathers (GFs) was i.d. injected with sterile SILIKON-1000 or phosphate-buffered saline (PBS) as injection control (8 and 3 birds/line, respectively; 10 µL/GF; 20 GFs/bird). Two GFs …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

The Intermediate And Remote Effects Of Acute Juvenile Stress Upon Auditory Fear Learning, Recognition Memory, And Anxiety Measures In A Rodent Model Of Ptsd, Laura J. Baron

Electronic Theses & Dissertations (2024 - present)

Abstract

Early-life stress is a risk factor for increased susceptibility to stress- and trauma-related disorders, including Posttraumatic Stress Disorder (PTSD). PTSD is characterized by exposure to trauma that results in exaggerated and extinction-resistant fear responses that persist for over a month. Understanding how the severity of early-life stressors in animal models impacts adult behavior, cognition, and underlying neurobiology could aid in the development of more effective PTSD treatments.

Various early-life stress models, especially in infant rats, have been used to induce stress and assess PTSD like-symptoms. Different stressors impact short- and long-term behavior, with some effects influenced by developmental timing …

Effects Of Essential Oils Alone Or In Combination With Monensin In An In Vitro Model Of Ruminal Acidosis, Jorge L. Bonilla Urbina

Electronic Theses and Dissertations

The objective of this study was to evaluate the effect of essential oils and/or monensin on the concentration of volatile fatty acids (VFA), ammonia, and pH in a ruminal acidosis in vitro model. Red Angus steers (n = 4; BW = 435 ± 9 kg) with ruminal and duodenal cannulas were allocated in a 4 × 4 Latin square design. Treatments were 1) Fed no essential oils (RB) or monensin sodium (MON:CON); 2) Fed RB at a rate of 14 g daily with no Mon (RB+); 3) Fed no RB and fed Mon at 400 mg daily (MON+); 4) Fed …

Tailwater Trout Population Dynamics, Connor Dylan Cunningham

Graduate Theses/Dissertations

Dams have been constructed throughout the world and provide several resources for humans (e.g., flood control, hydropower). These structures heavily alter the upstream and downstream environment. One consequence of these alterations is the creation of “tailwaters”, which can create cold-water habitat via hypolimnetic releases. In the southeast United States these tailwater environments are often managed for the recreational use of nonnative salmonids. The unnatural flow regimes of tailwaters affect the biotic and abiotic conditions and ultimately the dynamic rate functions (i.e., recruitment, growth, mortality) of the fish inhabiting them. As such, the goal of this research is to provide fisheries …

Use Of Fecal Inoculum From Easy And Hard Keeper Horses To Determine In-Vitro Digestibility Of Feedstuffs Used In Equine Diets, Leah Bills

Graduate Theses/Dissertations

Digestibility studies provide vital information for formulating ideal diets based on an animal’s ability to digest and absorb the available nutrients from the feed provided. The purpose of this study was to determine if there is a difference in the nutrient digestion of horses between those that have a difficult time maintaining a healthy body weight (body condition score at or below 5, hard keeper) and those that easily maintain a healthy body weight (body condition score of >5, easy keeper). Six mature geldings averaging 534.1 ± 47.1 kg were used in this study. During the experimental period horses were …

Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler

Masters Theses

Across the United States, fisheries managers and agencies continue to battle the spread of invasive bigheaded carp (Hypophthalmichthys spp.). Although previous research has identified environmental parameters such as temperature and flow to be triggers of spawning activity, many of these studies focus on larger rivers, rarely focusing on the specific thresholds or timing of when a spawning event takes place. I utilized the differing hydrology of the Illinois and Wabash Rivers and their tributaries to examine the differences in environmental parameters needed to trigger bigheaded carp reproduction. Three mainstem sites and three tributaries of both basins were sampled …

Pharmacological Safety Profiling Of Nigella Sativa (Ns) And Hibiscus Sabdariffa (Hs) Extracts In Zebrafish (Danio Rerio) Embryos: An Integrative Vet-Pharmacotoxicological Evaluation Of Lc₅₀, Teratogenicity, And Developmental Outcomes, Ahmed E. A. Mostafa

Mansoura Veterinary Medical Journal

Herbal medicines are deeply rooted in Egyptian and Middle Eastern traditions, with Nigella sativa (NS) and Hibiscus sabdariffa (HS) being among the most widely consumed remedies. Despite extensive pharmacological evidence of their therapeutic benefits, systematic safety validation remains limited. Conventional toxicity assessments predominantly rely on rodents and higher vertebrates, which are costly, time-consuming, and ethically challenging. This gap necessitates alternative models that align with the 3R principles (Reduction, Replacement, Refinement). In this study, the zebrafish (Danio rerio) embryo model was employed to evaluate the developmental toxicity and median lethal concentration (LC50) of NS and HS aqueous extracts. Zebrafish embryos at …

Evaluation Of Blue Catfish Populations In Four Midwestern Power Cooling Reservoirs, Tyler Joseph Murray

Masters Theses

Blue Catfish (Ictalurus furcatus) are the largest catfish in North America and as such angler desire for trophy fisheries have grown in addition to harvest fisheries. The Illinois Department of Natural Resources (IDNR) has stocked Blue Catfish into several reservoirs in the state including four that have powerplants that utilize the water for cooling and discharge it back into the reservoir at increased temperatures. This study explored the potential impacts of these warm water effluents on Blue Catfish population demographics, as well as whether management changes need to be implemented in these systems. I sampled Powerton, LaSalle, Springfield, …

Prospective Use Of Detection Dogs As A Screening Tool For Mycoplasma Ovipneumoniae In Bighorn Sheep (Ovis Canadensis), Michele C. Lovara

Electronic Theses and Dissertations

Mycoplasma ovipneumoniae is a bacterium that can spill over from domestic sheep (Ovis aries) to bighorn sheep (Ovis canadensis), with effects in bighorn sheep ranging from low to no lamb recruitment to whole-herd all-age die-offs from resulting pneumonia. Fast and reliable pathogen or disease detection is essential for managing outbreaks in bighorn sheep and emergences in affected populations. Detection dogs are increasingly being enlisted to close gaps and further complement various disease, pathogen and virus research and response efforts. This thesis explores the use of conservation detection dogs as a potential tool for M. ovipneumoniae surveillance in bighorn sheep through …

Phytogenic Feed Additives Pre- And Post-Calving And Effects Of Substituting Ddgs With Soybean Meal On Protein Metabolism In Beef Cattle, Grace H. Jardon

Electronic Theses and Dissertations

The objective of these studies was to evaluate nutritional strategies to improve beef cattle productivity through targeted supplementation and protein source manipulation. The first study investigated the effects of a phytogenic supplement (PHYT; a blend of turmeric, capsicum, and black pepper oleoresin) on colostrum yield and composition, calf passive immunity, and cow–calf performance. Twenty-three Angus-based cows were assigned to either a control group or received 250 or 500 mg/head/day of PHYT from 30 days pre-calving to 60 days post-calving. Supplementation with PHYT linearly increased colostrum fat and calf serum IgA concentrations. Milk fat, calf serum IgG, and colostrum IgM tended …

Investigating The Ruminal Metagenome Of Grass And Forage-Fed Bison To Uncover Metabolic Activities That Impact The Efficiency Of Plant Fiber Utilization, Anlly Fresno Rueda

Electronic Theses and Dissertations

This dissertation investigates the ruminal microbiome of American plains bison (Bison bison) to uncover the metabolic activities that enable efficient utilization of plant fiber, with a particular focus on bacterial composition and functional capabilities in response to forage quality. Using both 16S rRNA gene sequencing and metagenomic approaches, this research provides a comprehensive view of microbial diversity and metabolic potential in the rumen of bison, offering insights into their ecological resilience and efficiency in nutrient extraction. Chapter 2 focuses on the metabolic potential of bacterial communities in the rumen of bison fed a grass-based diet. Using high-throughput sequencing and metagenomic …

Identification Of Gut Bacterial Symbionts From Atlantic Salmon That Are Involved In Metabolizing Feed Ingredients Derived From Soybean Meal, Emily Fowler

Electronic Theses and Dissertations

In the aquaculture industry, plant-based protein sources have emerged as an economically and environmentally sustainable replacement for traditional animal protein sources. Yet, due to reduced digestibility, the presence of anti-nutritional factors, as well as an increased risk of inflammation of the digestive system, the inclusion of plant-based protein ingredients must be limited, especially in the diets of carnivorous fishes. To gain further insight on how to adapt the fish digestive tract to more effectively utilize or tolerate plant-based protein ingredients, slurry from cultured Atlantic Salmon (Salmo salar) was used as a proxy in an in vitro model system to identify …

Synchronization Protocols Utilizing Progesterone Implants Associated With Estrus Expression, Vaginal Microbiome, And Immune Response In Beef Cattle, Riley James Puck

Electronic Theses and Dissertations

This thesis aims to provide a deep understanding of presynchronization strategies and the varying factors affecting their efficacy. Beginning with a literature review of the estrous cycle and the endocrinology driving it. Building on this foundation, the physiological basis for estrous synchronization is described, followed by the advancement of pre-synchronization protocols designed to increase estrus and conception. Further, we will focus on the influence of estrus expression on insemination time and conception rates. Finally, how the immune system interacts with the conception process. In addition, the review goes beyond exogenous endocrine manipulation and dives into the emerging role of the …

One Rung At A Time: Climbing The Ladder Toward Aquatic Connectivity In Eastern South Dakota, Evan Spencer

Electronic Theses and Dissertations

Roads and streams are synonymous with their connection to movement, the acquisition of resources, and as pathways to new opportunities. However, the factors that define good roads and ecologically connected streams are rarely without conflict. Road infrastructure at stream-road crossings has the potential to fragment stream networks. Tube culverts, where streams pass under the road through metal pipes, are particularly concerning for stream connectivity. Undersized, aging, or inappropriately installed culverts can develop vertical drops at the outflow due to high velocities and stream bed scouring. The outlet drop can function as a barrier, preventing the upstream movement of fish. Due …

Extended Days On Feed: Influences On Growth Performance, Efficiency, And Carcass Characteristics Of Steers And Heifers Of Different Proportions Of Angus And Limousin Genetics, Grace Henley Olinger

Electronic Theses and Dissertations

Recently, cattle feeders have extended days on feed (DOF) resulting in hot carcass weights (HCW) exceeding the limits of existing serial slaughter datasets. This study's goal was to determine the impact extended DOF exerts on growth performance, feed efficiency, and carcass characteristics of steers and heifers with varying proportions of Angus (AN) and Limousin (LM) genetics. In 2022, commercial AN cows from two MT ranches were artificially inseminated to 9 sires from 3 breeds (AN, LM, and Limflex (LF)) with parentage confirmed by DNA. In 2023, 216 calves (sired by 72 AN, 73 LF, and 71 LM; 105 steers and …

Binoculars To Bytes: Development And Field Validation Of An Ai-Driven System For Avian Monitoring, Christian J. Dupree

Graduate Student Theses, Dissertations, & Professional Papers

Autonomous camera-trap arrays coupled with artificial-intelligence (AI) vision can lift bird monitoring beyond the spatial, temporal, and labor limits of traditional field surveys. We present Binoculars to Bytes (B2B), an open-source pipeline that turns 180–360° time-lapse imagery into analysis-ready avian data. At Freezout Lake Wildlife Management Area (Montana, USA) the system ran four-hour morning deployments during spring and fall migrations (2023–2024). A YOLO-NAS detector, incrementally refined with a “Specialized Localized Iterative Model” workflow, quadrupled local accuracy and, after confidence-based species-binning, cut false-positive rates in half. Daily AI species lists were benchmarked against contemporaneous eBird citizen-science checklists and recovered ≥ …

Ocean Acidification Mitigation: Potential Expansion Of Sav Restoration Strategy In The Chesapeake Bay, Cree N. Taylor

UROP Posters

Zostera marina is a species of marine macrophyte that has been largely prioritized in past submerged aquatic vegetation (SAV) restoration initiatives within the Chesapeake Bay because of its 1) previous abundance, 2) demonstrated carbon burial abilities, and 3) observed ability for meadows of Z. marina to ameliorate low pH conditions. Due to the temperature variability and poor water quality associated with anthropogenic disturbances in the Chesapeake Bay, Z. Marina beds have experienced several die-off events with limited capacity for regrowth. This systematic review explored the possible benefits of focusing future SAV recovery initiatives on ensuring a diverse variety of SAV …

The Effect Of Bait Types On Catch Performance In The Vietnamese Swimming Crab Trap Fishery, Nghiep K. Vu, Khanh Q. Nguyen, Luong T. Nguyen

Journal of Marine Science and Technology–Taiwan

Trapping is a primary fishing method to capture swimming crab species along the coast of Vietnam, because it has low ecological and environmental impacts. In trap fisheries, bait plays a primary role in determining the effectiveness of the gear and is one of the largest operational costs. Fishermen determine bait types based on their experience and what they can find, where trash fish caught by trawl fisheries is preferred and commonly used. However, the catch rates of swimming crab traps are typically low and highly variable and thus, there is considerable interest to improve the catchability. This study examined the …

Biological Control Of Ticks In Domestic Environments: Modeling The Potential Impact Of Entomopathogenic Fungi On The Transmission Of East Coast Fever In Cattle, Joseph Wang Ang A. Oundo, Nienke Hartemink, Mart C.M. De Jong, Constantianus J.M. Koenraadt, Shewit Kalayou, Daniel Masiga, Quirine Ten Bosch

All Peer-Reviewed Publications

Biological control of ticks using entomopathogenic fungi (EPF) is a highly desired alternative to chemical acaricides for the control of tick-borne pathogens. For Metarhizium anisopliae isolate ICIPE 7, one of these EPFs, efficacy against multiple tick species has been demonstrated in laboratory and field settings. However, we currently have little quantitative understanding of how EPFs can impact transmission. We developed a deterministic model of tick–host–pathogen interactions to explore how the effects of EPF on Rhipicephalus appendiculatus ticks may impact the transmission dynamics of East Coast fever (ECF) in cattle populations. We parameterized the multi-faceted effects of EPFs on tick dynamics …

Stress And Behavior In Beef Calves During Weaning, Kathleen E. Tierney

Theses and Dissertations

Animal welfare in commercial livestock operations has been an increasing topic of concern. It is well known that stress can have significant behavioral and physiological repercussions. It is especially important in commercial operations to reduce stress, as animal efficiency is the leading priority. In an effort to reduce stress on animals and improve welfare in livestock operations, several studies have evaluated common husbandry practices. One of the most widely studied practices is weaning, which encapsulates the process of removing the young from the dam. Several studies have evaluated the effects of weaning on beef cattle through a variety of behavioral …

Urban Coyotes As A Source Of Conflict With Humans: An Evaluation Of Common Management Practices, Paul D. Curtis, David L. Bergman, Angeline Barker, Daniel Bogan, Mary Ann Bonnell, Pierre Canac-Marquis, David Drake, Dave Kay, Brian F. Wakeling, H. Bryant White

Human–Wildlife Interactions Monographs

Wildlife often serves as a catalyst for conflict among stakeholders. Management actions that are intended to address sources of disagreement may initiate additional conflict. The Association of Fish and Wildlife Agencies Human–Wildlife Conflict Working Group initiated an effort to identify management actions commonly used to address unwanted coyote (Canis latrans) interactions within urban environments and identify the benefits and challenges associated with each action. Challenges with coyotes in urban environments include aggressive behavior toward humans and pets, attacks, disease exposure, and domestic livestock damage. Although elimination of all attractants would prove preventative in most instances, in practice this …

Food Finding Ability Does Not Predict Annual Survival In Winter Resident Birds, Aidan Hand

Honors Program: Senior Projects (Public)

Small non-migratory birds in temperate locations face many challenges to survive the winter. They must be able to find enough food, which is in decreased supply, to fuel the energetic demands of staying warm. Prior work has demonstrated that birds show individual variation in their foraging behaviors that could contribute to the discovery of new foraging patches, such as novel environment exploration. A better ability to discover new food sources could increase a bird’s chance of surviving the winter by increasing its access to new food patches. To test this hypothesis, we used radio frequency identification (RFID)-enabled bird feeders to …